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- Description
- Phenotype
- Genes & alleles
- Genotyping
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6(SJL)-Tg(MMTVtTA)1Mam/J (Changed: 04-MAY-07 ) C57BL/6J-Tg(MMTVtTA)1Mam/J (Changed: 26-APR-07 ) Type Congenic; Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N7
Generation DefinitionsDonating Investigator Lothar Hennighausen, National Institutes of Health Appearance
black
Related Genotype: a/aDescription
The MMTV-LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin in transgenic mice. When Tg(MMTVtTA)1Mam transgenic mice were mated to a second transgenic strains carrying either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE; tetO), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous reporter gene expression patterns were observed in mammary epithelial cells and basal cells of the epidermis. Lower reporter gene expression levels also were observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundred-fold and was abrogated after the administration of tetracycline. MMTV-tTA transgenic mice may be useful in experiments examining the roles of biological factors at defined developmental stages in the epithelial cells of salivary gland, seminal vesicle, mammary gland, and skin and in the Leydig cells of testes.Development
This strain was developed in the laboratory of Dr. Lothar Hennighausen at the National Institute of Diabetes, Digestive, & Kidney Diseases, National Institutes of Health. While the primary publication does not define the genetic background(s) used in generating these transgenic mice, correspondence with the donating investigator (in 1996) indicates that the transgene was introduced into a "B6/SJL" genetic background and was backcrossed four generations to B6 prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were backcrossed to C57BL/6J for at least one additional generation.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of tTA
View Strains carrying other alleles of tTA (38 strains)
004997 B6.Cg-Tg(MMTV-TGFBR2)7Hlm/J 007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 002375 B6;D2-Tg(MMTVTGFB1)46Hlm/J 010576 B6;SJL-Tg(MMTV-rtTA)4-1Jek/J 002459 B6D2-Tg(MMTVTGFA)254Rjc/J 002373 B6D2-Tg(MMTVTGFA)29Rjc/J 002870 B6SJL-Tg(Wnt1)1Hev/J 005038 FVB-Tg(MMTV-Erbb2)NK1Mul/J 004363 FVB.Cg-Tg(MMTV-vHaras)SH1Led/J 002953 FVB.Cg-Tg(MMTVTGFA)254Rjc/J 002934 FVB.Cg-Tg(Wnt1)1Hev/J 002437 FVB/N-Tg(MMTV-Notch4)3Rnc/J 002374 FVB/N-Tg(MMTV-PyVT)634Mul/J 002376 FVB/N-Tg(MMTVneu)202Mul/J 002933 FVB/NJ-Tg(MMTVTGFB1)46Hlm/J 003690 STOCK Tg(MMTV-Cdc37)1Stp/J 003337 STOCK Tg(MMTV-PIP)1Shu/J 003551 STOCK Tg(MMTV-cre)1Mam/J 003553 STOCK Tg(MMTV-cre)4Mam/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Cancer Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Expressing Strains
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(MMTVtTA)1Mam | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Lothar Hennighausen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | MMTV-tTA; | ||
| Mutation Made By | Lothar Hennighausen, National Institutes of Health | ||
| Strain of Origin | C57BL/6 and SJL | ||
| Site of Expression | Expresses tTA in the epithelial cells of secretory organs and skin. When mated to a tetop-lacz reporter mouse, nearly uniform expression of lacZ was found in seminal vesicle, salivary gland, and Leydig cells. More heterogeneous patterns of lacZ expression were observed in mammary epithelial cells of the epidermis. | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV | ||
| General Note |
Additional lines were generated and showed comparable tissue expression patterns. When transgenic mice were mated to mice transgenic for either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous patterns of reporter expression were observed in mammary epithelial cells and basal cells of the epidermis. Lower levels of reporter gene expression were also observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundredfold and was abrogated after the administration of tetracycline. In combination with Tg(BCR/ABL1)2Dgt, when tetracycline administration is stopped, bitransgenic mice are models for B cell acute lymphoblastic leukemia (ALL). (J:72377) | ||
| Molecular Note | The MMTV LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin. The tetracycline resistance gene (TetR) was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). [MGI Ref ID J:92586] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(tTA), Melt Curve Analysis
Tg(tTA), QPCR
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Hennighausen L; Wall RJ; Tillmann U; Li M; Furth PA. 1995. Conditional gene expression in secretory tissues and skin of transgenic mice using the MMTV-LTR and the tetracycline responsive system. J Cell Biochem 59(4):463-72. [PubMed: 8749716] [MGI Ref ID J:92586]
Additional References
Cui CY; Durmowicz M; Ottolenghi C; Hashimoto T; Griggs B; Srivastava AK; Schlessinger D. 2003. Inducible mEDA-A1 transgene mediates sebaceous gland hyperplasia and differential formation of two types of mouse hair follicles. Hum Mol Genet 12(22):2931-40. [PubMed: 14506134] [MGI Ref ID J:86628]
Tg(MMTVtTA)1Mam relatedCui CY; Durmowicz M; Ottolenghi C; Hashimoto T; Griggs B; Srivastava AK; Schlessinger D. 2003. Inducible mEDA-A1 transgene mediates sebaceous gland hyperplasia and differential formation of two types of mouse hair follicles. Hum Mol Genet 12(22):2931-40. [PubMed: 14506134] [MGI Ref ID J:86628]
Cui CY; Smith JA; Schlessinger D; Chan CC. 2005. X-linked anhidrotic ectodermal dysplasia disruption yields a mouse model for ocular surface disease and resultant blindness. Am J Pathol 167(1):89-95. [PubMed: 15972955] [MGI Ref ID J:99510]
Ewald D; Li M; Efrat S; Auer G; Wall RJ; Furth PA; Hennighausen L. 1996. Time-sensitive reversal of hyperplasia in transgenic mice expressing SV40 T antigen. Science 273(5280):1384-6. [PubMed: 8703072] [MGI Ref ID J:35417]
Harb JG; Chyla BI; Huettner CS. 2008. Loss of Bcl-x in Ph+ B-ALL increases cellular proliferation and does not inhibit leukemogenesis. Blood 111(7):3760-9. [PubMed: 18216295] [MGI Ref ID J:133520]
Hruska KS; Tilli MT; Ren S; Cotarla I; Kwong T; Li M; Fondell JD; Hewitt JA; Koos RD; Furth PA; Flaws JA. 2002. Conditional over-expression of estrogen receptor alpha in a transgenic mouse model. Transgenic Res 11(4):361-72. [PubMed: 12212839] [MGI Ref ID J:78639]
Huettner CS; Zhang P; Van Etten RA; Tenen DG. 2000. Reversibility of acute B-cell leukaemia induced by BCR-ABL1. Nat Genet 24(1):57-60. [PubMed: 10615128] [MGI Ref ID J:72377]
Letai A; Sorcinelli MD; Beard C; Korsmeyer SJ. 2004. Antiapoptotic BCL-2 is required for maintenance of a model leukemia. Cancer Cell 6(3):241-9. [PubMed: 15380515] [MGI Ref ID J:93549]
Omidvar N; Kogan S; Beurlet S; le Pogam C; Janin A; West R; Noguera ME; Reboul M; Soulie A; Leboeuf C; Setterblad N; Felsher D; Lagasse E; Mohamedali A; Thomas NS; Fenaux P; Fontenay M; Pla M; Mufti GJ; Weissman I; Chomienne C; Padua RA. 2007. BCL-2 and mutant NRAS interact physically and functionally in a mouse model of progressive myelodysplasia. Cancer Res 67(24):11657-67. [PubMed: 18089795] [MGI Ref ID J:130798]
Refaeli Y; Field KA; Turner BC; Trumpp A; Bishop JM. 2005. The protooncogene MYC can break B cell tolerance. Proc Natl Acad Sci U S A 102(11):4097-102. [PubMed: 15753301] [MGI Ref ID J:97189]
Refaeli Y; Young RM; Turner BC; Duda J; Field KA; Bishop JM. 2008. The B cell antigen receptor and overexpression of MYC can cooperate in the genesis of B cell lymphomas. PLoS Biol 6(6):e152. [PubMed: 18578569] [MGI Ref ID J:139351]
Tilli MT; Frech MS; Steed ME; Hruska KS; Johnson MD; Flaws JA; Furth PA. 2003. Introduction of estrogen receptor-alpha into the tTA/TAg conditional mouse model precipitates the development of estrogen-responsive mammary adenocarcinoma. Am J Pathol 163(5):1713-9. [PubMed: 14578170] [MGI Ref ID J:135395]
Tilli MT; Furth PA. 2003. Conditional mouse models demonstrate oncogene-dependent differences in tumor maintenance and recurrence. Breast Cancer Res 5(4):202-5. [PubMed: 12817992] [MGI Ref ID J:84503]
Young RM; Polsky A; Refaeli Y. 2009. TC-PTP is required for the maintenance of MYC-driven B-cell lymphomas. Blood 114(24):5016-23. [PubMed: 19755676] [MGI Ref ID J:155497]
Zeng H; Horie K; Madisen L; Pavlova MN; Gragerova G; Rohde AD; Schimpf BA; Liang Y; Ojala E; Kramer F; Roth P; Slobodskaya O; Dolka I; Southon EA; Tessarollo L; Bornfeldt KE; Gragerov A; Pavlakis GN; Gaitanaris GA. 2008. An inducible and reversible mouse genetic rescue system. PLoS Genet 4(5):e1000069. [PubMed: 18464897] [MGI Ref ID J:136987]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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