Strain Name: |
B6.Cg-Tg(MMTVtTA)1Mam/J |
|---|---|
Stock Number: |
002618 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Former Name |
B6(SJL)-Tg(MMTVtTA)1Mam/J (Changed: 04-MAY-07
) |
|
C57BL/6J-Tg(MMTVtTA)1Mam/J (Changed: 26-APR-07
) | |
| Genes & Alleles | tTA; MMTV; Tg(MMTVtTA)1Mam; |
Type JAX® GEMM® Strain - Congenic Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Mutant Strain Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Lothar Hennighausen, National Institutes of Health Generation N7 Appearance
black
Related Genotype: a/aStrain Description
The MMTV-LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin in transgenic mice. When Tg(MMTVtTA)1Mam transgenic mice were mated to a second transgenic strains carrying either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE; tetO), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous reporter gene expression patterns were observed in mammary epithelial cells and basal cells of the epidermis. Lower reporter gene expression levels also were observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundred-fold and was abrogated after the administration of tetracycline. MMTV-tTA transgenic mice may be useful in experiments examining the roles of biological factors at defined developmental stages in the epithelial cells of salivary gland, seminal vesicle, mammary gland, and skin and in the Leydig cells of testes.Strain Development
This strain was developed in the laboratory of Dr. Lothar Hennighausen at the National Institute of Diabetes, Digestive, & Kidney Diseases, National Institutes of Health. While the primary publication does not define the genetic background(s) used in generating these transgenic mice, correspondence with the donating investigator (in 1996) indicates that the transgene was introduced into a "B6/SJL" genetic background and was backcrossed four generations to B6 prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were backcrossed to C57BL/6J for at least one additional generation.
| Allele Symbol | Tg(MMTVtTA)1Mam | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Lothar Hennighausen | ||
| Common Name(s) | MMTV-tTA; | ||
| Mutation Made By | Lothar Hennighausen, National Institutes of Health | ||
| Strain of Origin | C57BL/6 and SJL | ||
| Site of Expression | Expresses tTA in the epithelial cells of secretory organs and skin. When mated to a tetop-lacz reporter mouse, nearly uniform expression of lacZ was found in seminal vesicle, salivary gland, and Leydig cells. More heterogeneous patterns of lacZ expression were observed in mammary epithelial cells of the epidermis. | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Promoter | MMTV, Mouse Mammary Tumor Virus, MMTV | ||
| General Note |
Additional lines were generated and showed comparable tissue expression patterns. When transgenic mice were mated to mice transgenic for either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous patterns of reporter expression were observed in mammary epithelial cells and basal cells of the epidermis. Lower levels of reporter gene expression were also observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundredfold and was abrogated after the administration of tetracycline. In combination with Tg(BCR/ABL1)2Dgt, when tetracycline administration is stopped, bitransgenic mice are models for B cell acute lymphoblastic leukemia (ALL). (J:72377) | ||
| Molecular Note | The MMTV LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin. The tetracycline resistance gene (TetR) was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). [MGI Ref ID J:92586] | ||
| Control | ||
|---|---|---|
| Noncarrier | ||
| 000664 C57BL/6J | ||
| Considerations for Choosing Controls | ||
Tg(tTA)
Tg(tTA) QPCR
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Strains carrying other alleles of tTA
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 003563 B6.Cg-Tg(tTALap)5Bjd/J 002709 B6;C3-Tg(TettTALuc)1Dgs/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 003272 STOCK Tg(tTALap)5Bjd/J 003271 STOCK Tg(tTAhCMV)3Bjd/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J View Strains carrying other alleles of tTA (19 strains)
Strains carrying other alleles of MMTV
004997 B6.Cg-Tg(MMTV-TGFBR2)7Hlm/J 007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 002375 B6;D2-Tg(MMTVTGFB1)46Hlm/J 002459 B6D2-Tg(MMTVTGFA)254Rjc/J 002373 B6D2-Tg(MMTVTGFA)29Rjc/J 002870 B6SJL-Tg(Wnt1)1Hev/J 005038 FVB-Tg(MMTV-Erbb2)NK1Mul/J 004363 FVB.Cg-Tg(MMTV-vHaras)SH1Led/J 002953 FVB.Cg-Tg(MMTVTGFA)254Rjc/J 002934 FVB.Cg-Tg(Wnt1)1Hev/J 002437 FVB/N-Tg(MMTV-Notch4)3Rnc/J 002374 FVB/N-Tg(MMTV-PyVT)634Mul/J 002376 FVB/N-Tg(MMTVneu)202Mul/J 002933 FVB/NJ-Tg(MMTVTGFB1)46Hlm/J 003690 STOCK Tg(MMTV-Cdc37)1Stp/J 003551 STOCK Tg(MMTV-cre)1Mam/J 003553 STOCK Tg(MMTV-cre)4Mam/J View Strains carrying other alleles of MMTV (17 strains)
Congenic Nomenclature
Tet Expression Systems
Research Tools
Cancer Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Expressing Strains)
Selected Reference(s)
Additional ReferencesHennighausen L; Wall RJ; Tillmann U; Li M; Furth PA. 1995. Conditional gene expression in secretory tissues and skin of transgenic mice using the MMTV-LTR and the tetracycline responsive system. J Cell Biochem 59(4):463-72. [PubMed: 8749716] [MGI Ref ID J:92586]
| Strain Name: | B6.Cg-Tg(MMTVtTA)1Mam/J |
| Stock Number: | 002618 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
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