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Strain Name:

B6.Cg-Tg(MMTVtTA)1Mam/J

Stock Number:

002618

Availability:

Repository-Cryopreserved

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Additional Licensing and Use Restrictions

Former Name      B6(SJL)-Tg(MMTVtTA)1Mam/J    (Changed: 04-MAY-07 )
      C57BL/6J-Tg(MMTVtTA)1Mam/J    (Changed: 26-APR-07 )
Genes & Alleles   tTA;   MMTV;   Tg(MMTVtTA)1Mam;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Lothar Hennighausen,   National Institutes of Health
GenerationN7

Appearance
black
Related Genotype: a/a

Strain Description
The MMTV-LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin in transgenic mice. When Tg(MMTVtTA)1Mam transgenic mice were mated to a second transgenic strains carrying either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE; tetO), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous reporter gene expression patterns were observed in mammary epithelial cells and basal cells of the epidermis. Lower reporter gene expression levels also were observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundred-fold and was abrogated after the administration of tetracycline. MMTV-tTA transgenic mice may be useful in experiments examining the roles of biological factors at defined developmental stages in the epithelial cells of salivary gland, seminal vesicle, mammary gland, and skin and in the Leydig cells of testes.

Strain Development
This strain was developed in the laboratory of Dr. Lothar Hennighausen at the National Institute of Diabetes, Digestive, & Kidney Diseases, National Institutes of Health. While the primary publication does not define the genetic background(s) used in generating these transgenic mice, correspondence with the donating investigator (in 1996) indicates that the transgene was introduced into a "B6/SJL" genetic background and was backcrossed four generations to B6 prior to arrival at The Jackson Laboratory. Upon arrival, mutant mice were backcrossed to C57BL/6J for at least one additional generation.

Gene & Allele Details

Allele Symbol Tg(MMTVtTA)1Mam
Allele Name transgene insertion 1, Lothar Hennighausen
Common Name(s) MMTV-tTA;
Mutation Made By Lothar Hennighausen,   National Institutes of Health
Strain of OriginC57BL/6 and SJL
Site of ExpressionExpresses tTA in the epithelial cells of secretory organs and skin. When mated to a tetop-lacz reporter mouse, nearly uniform expression of lacZ was found in seminal vesicle, salivary gland, and Leydig cells. More heterogeneous patterns of lacZ expression were observed in mammary epithelial cells of the epidermis.
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter MMTV, Mouse Mammary Tumor Virus, MMTV
General Note Additional lines were generated and showed comparable tissue expression patterns.

When transgenic mice were mated to mice transgenic for either lacZ or luciferase reporter genes coupled to tetracycline-responsive promoter elements (TRE), nearly uniform expression of the reporter was found in seminal vesicle, salivary gland, and Leydig cells of the double transgenic offspring. More heterogeneous patterns of reporter expression were observed in mammary epithelial cells and basal cells of the epidermis. Lower levels of reporter gene expression were also observed in a broad range of tissues. Transcriptional activation mediated by tTA was up to several hundredfold and was abrogated after the administration of tetracycline.

In combination with Tg(BCR/ABL1)2Dgt, when tetracycline administration is stopped, bitransgenic mice are models for B cell acute lymphoblastic leukemia (ALL). (J:72377)
Molecular Note The MMTV LTR was used to target expression of tetracycline-controlled transactivator protein (tTA) to the epithelial cells of secretory organs and skin. The tetracycline resistance gene (TetR) was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). [MGI Ref ID J:92586]

Control Information

  Control
   Noncarrier
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(tTA)
Tg(tTA) QPCR

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of tTA     (19 strains)

View Strains carrying other alleles of MMTV     (17 strains)

Additional Web Information

Congenic Nomenclature
Tet Expression Systems

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Cancer Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Expressing Strains)

References

Selected Reference(s)

Hennighausen L; Wall RJ; Tillmann U; Li M; Furth PA. 1995. Conditional gene expression in secretory tissues and skin of transgenic mice using the MMTV-LTR and the tetracycline responsive system. J Cell Biochem 59(4):463-72. [PubMed: 8749716]  [MGI Ref ID J:92586]

Additional References

Price and Supply Information

Strain Name: B6.Cg-Tg(MMTVtTA)1Mam/J
Stock Number: 002618

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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