Strain Name:

B6.129-Tgfb3tm1Doe/J

Stock Number:

002619

 Order this mouse

Availability:

Cryopreserved - Ready for recovery

Other products are available, see Purchasing Information for Cryopreserved Embryos

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6J
 
Donating Investigator Thomas Doetschman,   University of Arizona

Description
Mice homozygous for the Tgfb3tm1Doe mutation are not viable. Mice exhibit cleft palate and possibly developmental defects in the lung.

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Facebase: models
007664   129S-Efnb1tm1Sor/J
000646   A/J
000647   A/WySnJ
005709   B6.129-Skitm1Cco/J
007453   B6.129P2(Cg)-Dhcr7tm1Gst/J
010525   B6.129S-Notch2tm3Grid/J
010616   B6.129S1-Jag1tm1Grid/J
010546   B6.129S1-Jag2tm1Grid/J
010620   B6.129S1-Notch2tm1Grid/J
009387   B6.129S1-Osr1tm1Jian/J
009386   B6.129S1-Osr2tm1Jian/J
010621   B6.129S1-Snai1tm2.1Grid/J
010617   B6.129S1-Snai2tm1Grid/J
003865   B6.129S2-Itgavtm1Hyn/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
016902   B6.129S5-Irf6Gt(OST398253)Lex/J
003336   B6.129S7-Cdkn1ctm1Sje/J
012843   B6.129X1(Cg)-Slc32a1tm1.1Bgc/J
000026   B6.C3-Gli3Xt-J/J
004275   B6.Cg-Fignfi/Frk
012844   B6.Cg-Gad1tm1.1Bgc/J
006382   B6;129-Casktm1Sud/J
002711   B6;129-Gabrb3tm1Geh/J
004293   B6;129-Shhtm2Amc/J
012603   B6;129-Tgfbr2tm1Karl/J
010618   B6;129S-Jag1tm2Grid/J
010686   B6;129S-Snai1tm2Grid/J
009389   B6;129S1-Bambitm1Jian/J
010619   B6;129S1-Lfngtm1Grid/J
010547   B6;129S1-Notch3tm1Grid/J
010544   B6;129S1-Notch4tm1Grid/J
010722   B6;129S1-Snai2tm2Grid/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
003277   B6;129S7-Acvr2atm1Zuk/J
002788   B6;129S7-Fsttm1Zuk/J
002990   B6;129S7-Inhbatm1Zuk/J
000523   B6By.Cg-Eh/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000515   B6CBACa Aw-J/A-SfnEr/J
001434   C3HeB/FeJ x STX/Le-Mc1rE-so Gli3Xt-J Zeb1Tw/J
000252   DC/LeJ
005057   FVB.129-Kcnj2tm1Swz/J
012655   FVB.A-Irf6clft1/BeiJ
013100   FVB.C-Prdm16csp1/J
017437   FVB/N-Ckap5TgTn(sb-cHS4,Tyr)2320F-1Ove/J
017438   FVB/N-MidnTg(Tyr)2261EOve/J
017609   FVB/N-Rr16Tn(sb-Tyr)1HCebOve/J
017598   FVB/N-Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove/J
017608   FVB/N-Skor2Tn(sb-Tyr)1799B.CA7BOve/J
017436   FVB/N-Tapt1TgTn(sb-cHS4,Tyr)2508GOve/J
016870   FVB/NJ-Ap2b1Tg(Tyr)427Ove/EtevJ
017434   FVB;B6-Cramp1lTgTn(sb-rtTA,Tyr)2447AOve/J
017594   FVB;B6-Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve/J
017435   FVB;B6-SlmapTn(sb-rtTA)2426B.SB4Ove/J
003318   STOCK Shhtm1Amc/J
003102   STOCK Tgfb2tm1Doe/J
018624   STOCK Tgfb3tm2(Tgfb1)Vk/J
008469   STOCK Wnt9btm1.2Amc/J
View Facebase: models     (58 strains)

Strains carrying other alleles of Tgfb3
012719   STOCK Tgfb3tm1(cre)Vk/J
View Strains carrying other alleles of Tgfb3     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Arrhythmogenic Right Ventricular Dysplasia, Familial, 1; ARVD1   (TGFB3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tgfb3tm1Doe/Tgfb3+

        involves: 129P2/OlaHsd
  • digestive/alimentary phenotype
  • abnormal enterocyte apoptosis
    • mice exhibit reduced enterocyte apoptosis in the small intestine compared with wild-type mice   (MGI Ref ID J:76342)
    • however, enterocyte apoptosis in the colon is normal   (MGI Ref ID J:76342)
  • abnormal small intestinal villus morphology
    • villus length is increased compared to in wild-type mice   (MGI Ref ID J:76342)
  • cellular phenotype
  • abnormal enterocyte apoptosis
    • mice exhibit reduced enterocyte apoptosis in the small intestine compared with wild-type mice   (MGI Ref ID J:76342)
    • however, enterocyte apoptosis in the colon is normal   (MGI Ref ID J:76342)

Tgfb3tm1Doe/Tgfb3tm1Doe

        either: (involves: 129/Sv * 129P2/OlaHsd) or (involves: 129P2/OlaHsd * C57BL/6) or (involves: 129P2/OlaHsd * CF-1)
  • mortality/aging
  • complete neonatal lethality
    • all homozygotes die within 24 hrs after birth   (MGI Ref ID J:29901)
    • no homozygotes are recovered at 3 weeks of age, independent of genetic background   (MGI Ref ID J:29901)
  • behavior/neurological phenotype
  • absent gastric milk in neonates
    • homozygous mutant pups fail to suckle, as shown by absence of milk in their stomachs   (MGI Ref ID J:29901)
  • craniofacial phenotype
  • cleft palate
    • newborn homozygotes show a cleft palate phenotype of variable severity and type (anterior vs posterior) that is most severe on a C57BL/6-enriched background   (MGI Ref ID J:29901)
    • no associated cleft lip or gross abnormalities in cartilage, bone, brain, heart or other craniofacial structures (e.g. mandible) are observed   (MGI Ref ID J:29901)
    • on a 129P2/OlaHsd x C57BL/6 background, 46.7% of homozygotes exhibit a complete cleft palate while 53.3% display only a posterior cleft   (MGI Ref ID J:29901)
    • on a 129P2/OlaHsd x CF1 background, 89.3% homozygotes show a posterior cleft palate, 8.7% show anterior clefts (i.e. failure of fusion of primary and secondary palates), and ~2% exhibit complete clefts   (MGI Ref ID J:29901)
    • on a 129/Sv x 129P2/OlaHsd background, all homozygotes show only a posterior cleft palate   (MGI Ref ID J:29901)
    • persistence of medial edge epithelium during palatal shelf fusion
      • failure of palatal shelves to fuse appears to result from impaired adhesion of the apposing medial edge epithelia (MEE) and subsequent loss of the mid-line epithelial seam   (MGI Ref ID J:29901)
      • when fusion did occur, the MEE seam persisted in the mutant mice   (MGI Ref ID J:29901)
  • homeostasis/metabolism phenotype
  • cyanosis
    • newborn pups become cyanotic shortly after birth   (MGI Ref ID J:29901)
  • dehydration
    • newborn pups become dehydrated shortly after birth   (MGI Ref ID J:29901)
  • respiratory system phenotype
  • abnormal respiratory conducting tube morphology
    • newborn homozygotes display an abnormal terminal airway system   (MGI Ref ID J:29901)
  • respiratory distress
    • newborn pups exhibit gasping at ~4 hrs after birth   (MGI Ref ID J:29901)
  • digestive/alimentary phenotype
  • cleft palate
    • newborn homozygotes show a cleft palate phenotype of variable severity and type (anterior vs posterior) that is most severe on a C57BL/6-enriched background   (MGI Ref ID J:29901)
    • no associated cleft lip or gross abnormalities in cartilage, bone, brain, heart or other craniofacial structures (e.g. mandible) are observed   (MGI Ref ID J:29901)
    • on a 129P2/OlaHsd x C57BL/6 background, 46.7% of homozygotes exhibit a complete cleft palate while 53.3% display only a posterior cleft   (MGI Ref ID J:29901)
    • on a 129P2/OlaHsd x CF1 background, 89.3% homozygotes show a posterior cleft palate, 8.7% show anterior clefts (i.e. failure of fusion of primary and secondary palates), and ~2% exhibit complete clefts   (MGI Ref ID J:29901)
    • on a 129/Sv x 129P2/OlaHsd background, all homozygotes show only a posterior cleft palate   (MGI Ref ID J:29901)
    • persistence of medial edge epithelium during palatal shelf fusion
      • failure of palatal shelves to fuse appears to result from impaired adhesion of the apposing medial edge epithelia (MEE) and subsequent loss of the mid-line epithelial seam   (MGI Ref ID J:29901)
      • when fusion did occur, the MEE seam persisted in the mutant mice   (MGI Ref ID J:29901)

Tgfb3tm1Doe/Tgfb3tm1Doe

        involves: 129P2/OlaHsd * C57BL/6
  • nervous system phenotype
  • decreased neuron apoptosis
    • 2-fold in neurons of the substantia nigra pars compacta and ventral tegmental area   (MGI Ref ID J:117465)
  • loss of dopaminergic neurons
    • at P0 in the substantia nigra pars compacta and ventral tegmental area   (MGI Ref ID J:117465)
  • cellular phenotype
  • decreased neuron apoptosis
    • 2-fold in neurons of the substantia nigra pars compacta and ventral tegmental area   (MGI Ref ID J:117465)

Tgfb3tm1Doe/Tgfb3tm1Doe

        involves: 129 * C57BL/6J * ICR * Swiss Webster
  • craniofacial phenotype
  • cleft palate
    • incomplete, posterior   (MGI Ref ID J:136106)
  • respiratory system phenotype
  • *normal* respiratory system phenotype
    • mice exhibit normal lung morphology   (MGI Ref ID J:136106)
  • digestive/alimentary phenotype
  • cleft palate
    • incomplete, posterior   (MGI Ref ID J:136106)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Tgfb3tm1Doe related

Cancer Research
Growth Factors/Receptors/Cytokines

Developmental Biology Research
Craniofacial and Palate Defects
      congenital cleft palate

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Inflammation

Internal/Organ Research
Lung Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tgfb3tm1Doe
Allele Name targeted mutation 1, Thomas Doetschman
Allele Type Targeted (knock-out)
Common Name(s) TGF-beta3 null;
Mutation Made By Thomas Doetschman,   University of Arizona
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14.1
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Tgfb3, transforming growth factor, beta 3
Chromosome 12
Gene Common Name(s) ARVD; TGF-beta3; Tgfb-3;
Molecular Note A neomycin resistance cassette replaced exon 6, which encodes the active domain of the protein. RT-PCR analysis of RNA derived from whole E11.5 or E15.5 embryos did not detect any transcript produced from this allele in homozygous mice. [MGI Ref ID J:29901]

Genotyping

Genotyping Information

Genotyping Protocols

Tgfb3tm1Doe, Separated PCR
NEOTD (Generic Neo), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Proetzel G; Pawlowski SA; Wiles MV; Yin M; Boivin GP; Howles PN; Ding J; Ferguson MW; Doetschman T. 1995. Transforming growth factor-beta 3 is required for secondary palate fusion. Nat Genet 11(4):409-14. [PubMed: 7493021]  [MGI Ref ID J:29901]

Additional References

Cui XM; Chai Y; Chen J; Yamamoto T; Ito Y; Bringas P; Shuler CF. 2003. TGF-beta3-dependent SMAD2 phosphorylation and inhibition of MEE proliferation during palatal fusion. Dev Dyn 227(3):387-94. [PubMed: 12815624]  [MGI Ref ID J:84447]

Martinez-Alvarez C; Blanco MJ; Perez R; Rabadan MA; Aparicio M; Resel E; Martinez T; Nieto MA. 2004. Snail family members and cell survival in physiological and pathological cleft palates. Dev Biol 265(1):207-18. [PubMed: 14697364]  [MGI Ref ID J:87413]

Taya Y; O'Kane S; Ferguson MW. 1999. Pathogenesis of cleft palate in TGF-beta3 knockout mice. Development 126(17):3869-79. [PubMed: 10433915]  [MGI Ref ID J:56561]

Tgfb3tm1Doe related

Azhar M; Runyan RB; Gard C; Sanford LP; Miller ML; Andringa A; Pawlowski S; Rajan S; Doetschman T. 2009. Ligand-specific function of transforming growth factor beta in epithelial-mesenchymal transition in heart development. Dev Dyn 238(2):431-42. [PubMed: 19161227]  [MGI Ref ID J:144180]

Cui XM; Shiomi N; Chen J; Saito T; Yamamoto T; Ito Y; Bringas P; Chai Y; Shuler CF. 2005. Overexpression of Smad2 in Tgf-beta3-null mutant mice rescues cleft palate. Dev Biol 278(1):193-202. [PubMed: 15649471]  [MGI Ref ID J:96461]

Dunker N; Krieglstein K. 2003. Reduced programmed cell death in the retina and defects in lens and cornea of Tgfbeta2(-/-) Tgfbeta3(-/-) double-deficient mice. Cell Tissue Res 313(1):1-10. [PubMed: 12838410]  [MGI Ref ID J:105113]

Dunker N; Schmitt K; Krieglstein K. 2002. TGF-beta is required for programmed cell death in interdigital webs of the developing mouse limb. Mech Dev 113(2):111-20. [PubMed: 11960699]  [MGI Ref ID J:76541]

Dunker N; Schmitt K; Schuster N; Krieglstein K. 2002. The role of transforming growth factor beta-2, beta-3 in mediating apoptosis in the murine intestinal mucosa. Gastroenterology 122(5):1364-75. [PubMed: 11984523]  [MGI Ref ID J:76342]

Foitzik K; Paus R; Doetschman T; Dotto GP. 1999. The TGF-beta2 isoform is both a required and sufficient inducer of murine hair follicle morphogenesis. Dev Biol 212(2):278-89. [PubMed: 10433821]  [MGI Ref ID J:56937]

Galloway JL; Jones SJ; Mossey PA; Ellis IR. 2013. The control and importance of hyaluronan synthase expression in palatogenesis. Front Physiol 4:10. [PubMed: 23382716]  [MGI Ref ID J:195152]

Letterio JJ; Bottinger EP. 1998. TGF-beta knockout and dominant-negative receptor transgenic mice. Miner Electrolyte Metab 24(2-3):161-7. [PubMed: 9525700]  [MGI Ref ID J:46776]

Li J; Foitzik K; Calautti E; Baden H; Doetschman T; Dotto GP. 1999. TGF-beta3, but not TGF-beta1, protects keratinocytes against 12-O-tetradecanoylphorbol-13-acetate-induced cell death in vitro and in vivo. J Biol Chem 274(7):4213-9. [PubMed: 9933619]  [MGI Ref ID J:115227]

Martinez-Alvarez C; Blanco MJ; Perez R; Rabadan MA; Aparicio M; Resel E; Martinez T; Nieto MA. 2004. Snail family members and cell survival in physiological and pathological cleft palates. Dev Biol 265(1):207-18. [PubMed: 14697364]  [MGI Ref ID J:87413]

Martinez-Sanz E; Del Rio A; Barrio C; Murillo J; Maldonado E; Garcillan B; Amoros M; Fuerte T; Fernandez A; Trinidad E; Rabadan MA; Lopez Y; Martinez ML; Martinez-Alvarez C. 2008. Alteration of medial-edge epithelium cell adhesion in two Tgf-beta3 null mouse strains. Differentiation 76(4):417-30. [PubMed: 18431835]  [MGI Ref ID J:133954]

Mecha M; Rabadan MA; Pena-Melian A; Valencia M; Mondejar T; Blanco MJ. 2008. Expression of TGF-betas in the embryonic nervous system: analysis of interbalance between isoforms. Dev Dyn 237(6):1709-17. [PubMed: 18498095]  [MGI Ref ID J:136358]

Memon MA; Anway MD; Covert TR; Uzumcu M; Skinner MK. 2008. Transforming growth factor beta (TGFbeta1, TGFbeta2 and TGFbeta3) null-mutant phenotypes in embryonic gonadal development. Mol Cell Endocrinol 294(1-2):70-80. [PubMed: 18790002]  [MGI Ref ID J:145458]

Mu Z; Yang Z; Yu D; Zhao Z; Munger JS. 2008. TGFbeta1 and TGFbeta3 are partially redundant effectors in brain vascular morphogenesis. Mech Dev 125(5-6):508-16. [PubMed: 18343643]  [MGI Ref ID J:136106]

Pangas SA. 2012. Regulation of the ovarian reserve by members of the transforming growth factor beta family. Mol Reprod Dev 79(10):666-79. [PubMed: 22847922]  [MGI Ref ID J:190579]

Pryce BA; Watson SS; Murchison ND; Staverosky JA; Dunker N; Schweitzer R. 2009. Recruitment and maintenance of tendon progenitors by TGF{beta} signaling are essential for tendon formation. Development 136(8):1351-61. [PubMed: 19304887]  [MGI Ref ID J:147280]

Roussa E; Wiehle M; Dunker N; Becker-Katins S; Oehlke O; Krieglstein K. 2006. Transforming growth factor beta is required for differentiation of mouse mesencephalic progenitors into dopaminergic neurons in vitro and in vivo: ectopic induction in dorsal mesencephalon. Stem Cells 24(9):2120-9. [PubMed: 16741229]  [MGI Ref ID J:174490]

Saika S; Saika S; Liu CY; Azhar M; Sanford LP; Doetschman T; Gendron RL; Kao CW; Kao WW. 2001. TGFbeta2 in corneal morphogenesis during mouse embryonic development. Dev Biol 240(2):419-32. [PubMed: 11784073]  [MGI Ref ID J:73681]

Sasaki Y; O'kane S; Dixon J; Dixon MJ; Ferguson MW. 2007. Temporal and spatial expression of Pax9 and Sonic hedgehog during development of normal mouse palates and cleft palates in TGF-beta3 null embryos. Arch Oral Biol 52(3):260-7. [PubMed: 17097601]  [MGI Ref ID J:117758]

Taya Y; O'Kane S; Ferguson MW. 1999. Pathogenesis of cleft palate in TGF-beta3 knockout mice. Development 126(17):3869-79. [PubMed: 10433915]  [MGI Ref ID J:56561]

Tesseur I; Wyss-Coray T. 2006. A role for TGF-beta signaling in neurodegeneration: evidence from genetically engineered models. Curr Alzheimer Res 3(5):505-13. [PubMed: 17168649]  [MGI Ref ID J:125213]

Tudela C; Formoso MA; Martinez T; Perez R; Aparicio M; Maestro C; Del Rio A; Martinez E; Ferguson M; Martinez-Alvarez C. 2002. TGF-beta3 is required for the adhesion and intercalation of medial edge epithelial cells during palate fusion. Int J Dev Biol 46(3):333-6. [PubMed: 12068957]  [MGI Ref ID J:80255]

Vogel T; Ahrens S; Buttner N; Krieglstein K. 2010. Transforming growth factor beta promotes neuronal cell fate of mouse cortical and hippocampal progenitors in vitro and in vivo: identification of Nedd9 as an essential signaling component. Cereb Cortex 20(3):661-71. [PubMed: 19587023]  [MGI Ref ID J:174166]

Xu X; Han J; Ito Y; Bringas P Jr; Deng C; Chai Y. 2008. Ectodermal Smad4 and p38 MAPK are functionally redundant in mediating TGF-beta/BMP signaling during tooth and palate development. Dev Cell 15(2):322-9. [PubMed: 18694570]  [MGI Ref ID J:140401]

Zhang J; Pho V; Bonasera SJ; Holtzman J; Tang AT; Hellmuth J; Tang S; Janak PH; Tecott LH; Huang EJ. 2007. Essential function of HIPK2 in TGFbeta-dependent survival of midbrain dopamine neurons. Nat Neurosci 10(1):77-86. [PubMed: 17159989]  [MGI Ref ID J:117465]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2250.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2925.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   Wild-type from the colony
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.2)