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Strain Name:

B6.129S2-Tnfrsf1btm1Mwm/J

Stock Number:

002620

Availability:

Repository- Live

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General Terms and Conditions

Former Name      TNFR2 (2733)    (Changed: 15-DEC-04 )
      Tnfr2    (Changed: 15-DEC-04 )
Genes & Alleles   Tnfrsf1b;   Tnfrsf1btm1Mwm;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S2 via D3 ES cell line
Donating Investigator Mark Moore,   Deltagen
GenerationN10F27 (20-DEC-07)

Appearance
black
Related Genotype: a/a

Strain Description
Mice homozygous for a Tnfrsf1btm1Mwm targeted mutation (formerly Tnfr2tm1Mwm, p75 deficient) are viable and fertile. Homozygous mutant mice show normal T-cell development and activity, but are resistant to TNF-induced cell death. Subcutaneous injections of TNF into homozygotes elicit much less tissue necrosis.

Strain Development
This strain was developed by using homologous recombination techniques to target the Tnfr2 gene. The inserted construct contained a neomycin resistance gene under the control of the Pgk promoter and was inserted into the second exon of the Tnfr2 gene.

Mammalian Phenotype Terms assigned by genotype

Tnfrsf1btm1Mwm/Tnfrsf1btm1Mwm

        B6.129S2-Tnfrsf1btm1Mwm/J
  • immune system phenotype
  • decreased T cell proliferation (MGI Ref ID J:107039)
    • proliferation of lymph node T cells is reduced in response to anti-CD3e monoclonal antibody stimulation as compared to wild-type
    • purified CD8+ T cells require a 5 fold greater anti-CD3 stimulation for tritiated thymidine incorporation equivalent to wild type, however proliferation is increased in the presence of IL2
    • purified CD4+ T cells require a greater anti-CD3 stimulation for tritiated thymidine incorporation, however proliferation is increased in the presence of IL2
  • decreased interferon-gamma secretion (MGI Ref ID J:107039)
    • CD8+ T cells produce less interferon gamma following anti-CD3 stimulation than control cells; the result of fewer cells expressing interferon gamma as well as a decrease in the amount produced per cell
  • decreased interleukin-2 secretion (MGI Ref ID J:107039)
    • CD8+ T cells exhibit a reduction in IL2 secretion following anti-CD3 stimulation
  • hematopoietic system phenotype
  • decreased T cell proliferation (MGI Ref ID J:107039)
    • proliferation of lymph node T cells is reduced in response to anti-CD3e monoclonal antibody stimulation as compared to wild-type
    • purified CD8+ T cells require a 5 fold greater anti-CD3 stimulation for tritiated thymidine incorporation equivalent to wild type, however proliferation is increased in the presence of IL2
    • purified CD4+ T cells require a greater anti-CD3 stimulation for tritiated thymidine incorporation, however proliferation is increased in the presence of IL2

Tnfrsf1btm1Mwm/Tnfrsf1btm1Mwm

        B6.129S2-Tnfrsf1btm1Mwm
  • nervous system phenotype
  • abnormal central nervous system regeneration (MGI Ref ID J:71178)
    • 7 days after traumatic spinal cord injury increased cell death is seen in the center of the lesion and the rostrocaudal spread of the lesion is increased compared to wild type
    • 4 weeks after traumatic spinal cord injury locomotor performance is significantly more impaired but lesions are not significantly longer than in wild type mice

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tnfrsf1btm1Mwm/Tnfrsf1btm1Mwm

        involves: 129S2/SvPas * C57BL/6J
  • immune system phenotype
  • abnormal immune system physiology (MGI Ref ID J:21815)
    • decreased sensitivity to exogenous tumor necrosis factor (TNF), however, death occurs at high doses
    • increased susceptibility to bacterial infection (MGI Ref ID J:21815)
      • increased mortality as compared to wild type following injection with Listeria monocytogenes

Tnfrsf1btm1Mwm/Tnfrsf1btm1Mwm

        involves: 129S2/SvPas * C57BL/6J * NOD
  • immune system phenotype
  • decreased susceptibility to autoimmune diabetes (MGI Ref ID J:64051)
    • none of the animals develop diabetes until 24 weeks (no blood glucose measure of >300 mg/dl)
  • periinsulitis (MGI Ref ID J:64051)
    • at 5 weeks, mice exhibit periinsulits, while transgenic littermates show severe insulitis
  • endocrine/exocrine gland phenotype
  • periinsulitis (MGI Ref ID J:64051)
    • at 5 weeks, mice exhibit periinsulits, while transgenic littermates show severe insulitis
  • digestive/alimentary phenotype
  • periinsulitis (MGI Ref ID J:64051)
    • at 5 weeks, mice exhibit periinsulits, while transgenic littermates show severe insulitis

Gene & Allele Details

Allele Symbol Tnfrsf1btm1Mwm
Allele Name targeted mutation 1, Mark Moore
Common Name(s) TNF-R2-; TNFR II -; TNFR20; TNFRp75-; p75-; p75TNFR2-;
Mutation Made By Mark Moore,   Deltagen
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Tnfrsf1b, tumor necrosis factor receptor superfamily, member 1b
Chromosome 4
Gene Common Name(s) CD120b; TBPII; TNF-R-II; TNF-R2; TNF-R75; TNF-alphaR2; TNFBR; TNFR2; TNFR80; TNFRII; Tnfr-1; Tnfr2; p75; p75 TNFR; p75TNFR; tumor necrosis factor receptor 1; tumor necrosis factor receptor 2;
Molecular Note Exon 2, which codes for the signal peptide region, was disrupted by the insertion of a neomycin cassette. To confirm that the gene was correctly targeted, mice were challenged with lipopolysaccharide (LPS) which results in shedding of the Tnfrsf1b extracellular domian in wild type mice. No soluble Tnfrsf1b was detected in the serum of homozygous mutant animals. [MGI Ref ID J:21815]

Control Information

  Allele   Control
 Tnfrsf1btm1Mwm  000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tnfrsf1btm1Mwm

Colony Maintenance

Breeding & HusbandryThis strain was generated on a mixed 129 and C57BL/6J background. It has been backcrossed to C57BL/6 six times. Homozygotes are viable and fertile. The strain is maintained by mating homozygous siblings. Expected coat color from breeding:Black
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying other alleles of Tnfrsf1b
003246   B6.129S7-Tnfrsf1btm1Imx/J
003243   B6;129S-Tnfrsf1atm1Imx Tnfrsf1btm1Imx/J
View Strains carrying other alleles of Tnfrsf1b     (2 strains)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Animal Health Reports

Room Number           AX11

Research Applications

This mouse can be used to support research in many areas including:

Tnfrsf1btm1Mwm related

Apoptosis Research
Death Receptors

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology and Inflammation Research
Growth Factors/Receptors/Cytokines
Inflammation

References

Selected Reference(s)

Erickson SL; de Sauvage FJ; Kikly K; Carver-Moore K; Pitts-Meek S; Gillett N; Sheehan KC; Schreiber RD; Goeddel DV; Moore MW. 1994. Decreased sensitivity to tumour-necrosis factor but normal T-cell development in TNF receptor-2-deficient mice. Nature 372(6506):560-3. [PubMed: 7990930]  [MGI Ref ID J:21815]

Additional References

Price and Supply Information

Strain Name: B6.129S2-Tnfrsf1btm1Mwm/J
Stock Number: 002620

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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