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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
Strain Information
Former Names B6;SJL-TgN(tetoplacZ)2Mam (Changed: 15-DEC-04 ) Type Mutant Stock; Transgenic; Species laboratory mouse Donating Investigator Lothar Hennighausen, National Institutes of Health Description
B6;SJL-Tg(tetoplacZ)2Mam transgenic mice serve as reporter/tester mice for transgenic mice expressing the tetracycline-controlled transactivator proteins tTA or rtTA. This strain carries a beta-galactosidase (lacZ) reporter gene under the control of a tetracycline-responsive promoter element (TRE; tetO), consisting of the human cytomegalovirus early promoter fused to tet operator sequences. When these mice are mated with transgenic mice expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawl, if tTA is used) of tetracycline, or its analog, doxycyline. Beta-galactosidase activity is not observed in the absence of tTA or rtTA transgene.Development
A transgenic construct containing a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal was injected into fertilized B6;SJL mouse eggs. Founder animals were obtained.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (174 strains)
Additional Web Information
Fluorescent Proteins/lacZ Systems
Tet Expression Systems
Phenotype
Phenotype Information
Research Applications
This mouse can be used to support research in many areas including:
lacZ relatedResearch Tools
lacZ Expression
Genetics Research (Mutagenesis and Transgenesis: Tetop Tet System)
Tet Expression Systems (tTA/rtTA Responsive Strains)
Research Tools
lacZ Expression
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(tetop-lacZ)2Mam | ||
|---|---|---|---|
| Allele Name | transgene insertion 2, Lothar Hennighausen | ||
| Common Name(s) | TetO-beta-gal; | ||
| Strain of Origin | involves: C57BL/6 * SJL | ||
| Site of Expression | When crossed with a strain expressing rTA or rtTA, expression of the transgene may be induced in the bitransgenic progeny by the administration or withdrawal of tetracycline or its analog, doxycycline. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | tetO, tet operator, | ||
| General Note |
Four lines were produced (G1-G4). In mice carrying both this transgene and a transgene expressing tTA or rtTA, lacZ transcription may be induced by the administration (or withdrawal, if tTA is used) of tetracycline, or its analog, doxycyline. No beta-galactosidase activity is observed in the absence of a tTA transgene. | ||
| Molecular Note | The construct contains a nuclear localization signal, the beta-galactosidase structural gene, an intron, and a poly(A) signal. Expression of beta-galactosidase is controlled by a tetracycline-responsive promoter element (TRE) consisting of the human cytomegalovirus early promoter fused to tet operator sequences. No beta-galactosidase activity was observed in the absence of a tTA transgene. [MGI Ref ID J:92587] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, MCA, vers. 2
Generic LacZ, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Furth PA; St Onge L; Boger H; Gruss P; Gossen M; Kistner A; Bujard H; Hennighausen L. 1994. Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc Natl Acad Sci U S A 91(20):9302-6. [PubMed: 7937760] [MGI Ref ID J:92587]
Additional References
Hennighausen L; Wall RJ; Tillmann U; Li M; Furth PA. 1995. Conditional gene expression in secretory tissues and skin of transgenic mice using the MMTV-LTR and the tetracycline responsive system. J Cell Biochem 59(4):463-72. [PubMed: 8749716] [MGI Ref ID J:92586]
Tg(tetop-lacZ)2Mam related
Dunbar ME; Dann P; Brown CW; Van Houton J; Dreyer B; Philbrick WP; Wysolmerski JJ. 2001. Temporally regulated overexpression of parathyroid hormone-related protein in the mammary gland reveals distinct fetal and pubertal phenotypes. J Endocrinol 171(3):403-16. [PubMed: 11739006] [MGI Ref ID J:127904]Jaubert J; Patel S; Cheng J; Segre JA. 2004. Tetracycline-regulated transactivators driven by the involucrin promoter to achieve epidermal conditional gene expression. J Invest Dermatol 123(2):313-8. [PubMed: 15245431] [MGI Ref ID J:134564]
Ponomareva ON; Ma H; Vock VM; Ellerton EL; Moody SE; Dakour R; Chodosh LA; Rimer M. 2006. Defective neuromuscular synaptogenesis in mice expressing constitutively active ErbB2 in skeletal muscle fibers. Mol Cell Neurosci 31(2):334-45. [PubMed: 16278083] [MGI Ref ID J:106884]
Sanbe A; Gulick J; Hanks MC; Liang Q; Osinska H; Robbins J. 2003. Reengineering inducible cardiac-specific transgenesis with an attenuated myosin heavy chain promoter. Circ Res 92(6):609-16. [PubMed: 12623879] [MGI Ref ID J:125131]
Shakya R; Jho EH; Kotka P; Wu Z; Kholodilov N; Burke R; D'Agati V; Costantini F. 2005. The role of GDNF in patterning the excretory system. Dev Biol 283(1):70-84. [PubMed: 15890330] [MGI Ref ID J:99351]
Stanger BZ; Tanaka AJ; Melton DA. 2007. Organ size is limited by the number of embryonic progenitor cells in the pancreas but not the liver. Nature 445(7130):886-91. [PubMed: 17259975] [MGI Ref ID J:118596]
Tilli MT; Furth PA. 2003. Conditional mouse models demonstrate oncogene-dependent differences in tumor maintenance and recurrence. Breast Cancer Res 5(4):202-5. [PubMed: 12817992] [MGI Ref ID J:84503]
Whisenhunt TR; Yang X; Bowe DB; Paterson AJ; Van Tine BA; Kudlow JE. 2006. Disrupting the enzyme complex regulating O-GlcNAcylation blocks signaling and development. Glycobiology 16(6):551-63. [PubMed: 16505006] [MGI Ref ID J:134284]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $1900.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
*Price(s) in US dollars ($)
Weeks of Age Price* Gender Cryorecovery Fee $2470.00
Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
|
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 100012 B6SJLF1/J | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
View JAX® Mice & Services Conditions of Use.
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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