Description

Strain Information

Former Names C57BL/6JSmn-Dstncorn1-2J    (Changed: 15-DEC-04 ) Type Mutant Strain; Additional information on Genetically Engineered Mutant Mice. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse

Description
Histological assessment of corneas from mice homozygous for the Dstn ^corn1-2J mutation reveals focal epithelial thickening and enlarged surface epithelial cells with degenerating nuclei. These mice lack the neovascularization found in mice homozygous for the Dstn ^corn1 mutation and have a more mild corneal epithelial phenotype. Preliminary assessment indicates that this variation in phenotype results from the distinct mutations in Dstn, not from differences in genetic background. In both mutants the corneal epithelial cells show increased levels and altered organizational patterns of filamentous actin. In the Dstn^corn1 mutants this alteration to filamentous actin has been shown to result in accelerated proliferation of basal corneal epithelial cells. (Smith et al., 1996; Wang et al., 2001; Ikeda et al., 2003.)

Development
The corn1-2J allele of Dstn was identified in the C57BL/6JSmn inbred strain at The Jackson Laboratory in approximately 1991.

Control Information

	Control
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Dstn

001649

A.BY H2bc H2-T18f/SnJ-Dstncorn1/J

View Strains carrying other alleles of Dstn     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Dstn^corn1-2J/Dstn^corn1-2J

        C57BL/6JSmn-Dstn^corn1-2J/J

• vision/eye phenotype
• *normal* vision/eye phenotype (MGI Ref ID J:139239)
  • unlike Dstn^corn1 homozygotes neutrophil and macrophage infiltration of the cornea is not seen
  • unlike Dstn^corn1 homozygotes corneal vascularization is not seen
• abnormal corneal epithelium morphology (MGI Ref ID J:83125)
  • epithelial lesion characterized by focal epithelial thickening and enlarged surface
  • epithelial cells with degenerating nuclei
  • phenotype is milder than in Dstn^corn1 homozygotes

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Dstn^corn1-2J related

Cell Biology Research
Cell Cycle Regulation
Genes Regulating Growth and Proliferation
Post-translational Processing
Signal Transduction

Developmental Biology Research
Eye Defects

Sensorineural Research
Cataracts (cortical)
Eye Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Dstncorn1-2J
Allele Name		corneal disease 1, 2 Jackson
Allele Type		Spontaneous
Strain of Origin		C57BL/6J
Gene Symbol and Name		Dstn, destrin
Chromosome		2
Gene Common Name(s)		2610043P17Rik; ACTDP; ADF; AU042046; RIKEN cDNA 2610043P17 gene; bA462D18.2; corn1; corneal disease 1; expressed sequence AU042046; sid23p;
Molecular Note		The corn1-2J mouse was identified as an allele of corn1 by complementation analysis. The mutation was identifed as a C to T transition at nucleotide position 316 in exon 3. This mutation results in a Pro106Ser amino acid change that is predicted to affect the binding of the protein to F- and G- actin. [MGI Ref ID J:83125]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Ikeda S; Cunningham LA; Boggess D; Hobson CD; Sundberg JP; Naggert JK; Smith RS; Nishina PM. 2003. Aberrant actin cytoskeleton leads to accelerated proliferation of corneal epithelial cells in mice deficient for destrin (actin depolymerizing factor). Hum Mol Genet 12(9):1029-37. [PubMed: 12700171]  [MGI Ref ID J:83125]

Dstn^corn1-2J related

Ikeda S; Cunningham LA; Boggess D; Hobson CD; Sundberg JP; Naggert JK; Smith RS; Nishina PM. 2003. Aberrant actin cytoskeleton leads to accelerated proliferation of corneal epithelial cells in mice deficient for destrin (actin depolymerizing factor). Hum Mol Genet 12(9):1029-37. [PubMed: 12700171]  [MGI Ref ID J:83125]

Verdoni AM; Smith RS; Ikeda A; Ikeda S. 2008. Defects in actin dynamics lead to an autoinflammatory condition through the upregulation of CXCL5. PLoS ONE 3(7):e2701. [PubMed: 18628996]  [MGI Ref ID J:139239]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           A1

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Eye Mutant Resource within the Mouse Mutant Resource collection.

Control Information

	Control
	000664 C57BL/6J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

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