Strain Name:

B6.129-Htr2ctm1Jul/J

Stock Number:

002627

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129
 
Donating InvestigatorDr. David Julius,   Univ of California at San Francisco

Description
Homozygous mice show spontaneous and infrequent epileptic seizures, sometimes resulting in death. They also show overeating behavior and an increase in the accumulation of white adipose tissue.

Development
This strain was developed in the laboratory of Dr. David Julius at University of California at San Francisco. A routine Genetic Quality Control assessment of the strain's genetic background was performed (6/2005) using a panel of 27 Single Nucleotide Polymorphism (SNP) markers. The SNP markers used distinguish between the inbred mouse strain backgrounds found at The Jackson Laboratory (TJL). Our findings indicate that portions of chromosome 1 (region of 52.0 cM) and chromosome X (region of 21.9 cM) are derived from the 129 donor strain genetic background. The targeted gene is located at 66.15 cM on the X chromosome. This strain was imported into the Induced Mutant Resource at TJL in 1997. It was assigned a congenic strain designation based upon guidelines offered by International Committee on Standardized Genetic Nomenclature for Mice, in conjunction with information provided by the donating investigator that the imported mice had been backcrossed to C57BL/6J at least 5 times (N5).

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Htr2c
015821   B6.129-Htr2ctm1Jke/J
View Strains carrying other alleles of Htr2c     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Diabetes Mellitus, Noninsulin-Dependent; NIDDM
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Htr2ctm1Jul/Y

        B6.129S-Htr2ctm1Jul
  • behavior/neurological phenotype
  • abnormal response to new environment
    • hemizygotes show reduced aversion to a novel environment; the latencies to emerge from a small dark enclosure into a brightly lit open field are reduced in mutants compared to wild-type   (MGI Ref ID J:51567)
  • abnormal spatial learning
    • hemizygotes show abnormal performance in the Morris water maze, failing to show a preference for the trained site in probe trials following training   (MGI Ref ID J:51567)
    • however, contextual conditioning is normal   (MGI Ref ID J:51567)
  • polyphagia
    • both young (12-14 weeks) and older (39-43 weeks) male hemizygotes show a significant increase in daily food intake relative to wild-type controls   (MGI Ref ID J:50262)
    • however, no significant differences are observed in plasma glucose, corticosterone, triglycerides or FFAs at either age   (MGI Ref ID J:50262)
  • adipose tissue phenotype
  • increased epididymal fat pad weight
    • at 39-43 weeks of age   (MGI Ref ID J:50262)
  • increased renal fat pad weight
    • at 39-43 weeks of age   (MGI Ref ID J:50262)
  • increased white adipose tissue amount
    • on a standard chow diet, male hemizygotes show an age-dependent increase in white adipose tissue (WAT) deposits relative to wild-type controls   (MGI Ref ID J:50262)
    • at 39-43 weeks of age, epididymal, perirenal and subcutaneous WAT weights are increased by 40-64% relative to wild-type levels   (MGI Ref ID J:50262)
  • growth/size/body phenotype
  • increased susceptibility to age related obesity
    • on a standard chow diet, male hemizygotes develop late onset obesity at >20 weeks of age   (MGI Ref ID J:50262)
    • by 42 weeks of age, hemizygotes show a ~30% increase in body weight relative to wild-type controls   (MGI Ref ID J:50262)
  • increased susceptibility to diet-induced obesity
    • when fed a high-fat diet for 9 weeks, male hemizygotes display a significantly higher gain weight than similarly treated wild-type mice; however, no differences in plasma FFA or triglyceride levels are observed   (MGI Ref ID J:50262)
  • homeostasis/metabolism phenotype
  • abnormal response/metabolism to endogenous compounds
    • following an i.p. injection of 2.5 mg/kg of leptin, 38-46 week old male hemizygotes mice exhibit a smaller decrease in food intake and body weight relative to wild-type controls; however, leptin resistance can be overcome by a higher dose (5 mg/kg) and is therefore only partial in obese hemizygotes   (MGI Ref ID J:50262)
    • no differences in the effects of either leptin dose (2.5 or 5 mg/kg) on food intake and body weight are observed in younger male hemizygotes at 11-13 weeks of age   (MGI Ref ID J:50262)
  • abnormal tumor necrosis factor level
    • obese hemizygotes display markedly increased white adipose tissue TNF mRNA levels, despite normal plasma FFA and corticosterone levels   (MGI Ref ID J:50262)
  • hyperglycemia
    • when fed a high-fat diet for 9 weeks, male hemizygotes display plasma glucose levels in the diabetic range (>300 mg/dl); however, no hyperlipidemia is observed   (MGI Ref ID J:50262)
  • impaired glucose tolerance
    • at 120 min after a single i.p. injection of D-glucose (1 gm/kg), obese male hemizygotes display significantly higher blood glucose levels than wild-type controls, indicating impaired glucose tolerance   (MGI Ref ID J:50262)
  • increased circulating insulin level
    • at 39-43 weeks of age, male hemizygotes display a 7-fold increase in plasma insulin levels relative to wild-type controls; however, plasma insulin levels are normal at 12-14 weeks of age   (MGI Ref ID J:50262)
    • when fed a high-fat diet for 9 weeks, male hemizygotes display hyperinsulinemia relative to similarly treated wild-type mice   (MGI Ref ID J:50262)
  • increased circulating leptin level
    • at 39-43 weeks of age, male hemizygotes display a 2.5-fold increase in plasma leptin levels relative to wild-type controls; however, plasma leptin levels are normal at 12-14 weeks of age   (MGI Ref ID J:50262)
    • when fed a high-fat diet for 9 weeks, male hemizygotes display hyperleptinemia relative to similarly treated wild-type mice   (MGI Ref ID J:50262)
  • increased susceptibility to diet-induced obesity
    • when fed a high-fat diet for 9 weeks, male hemizygotes display a significantly higher gain weight than similarly treated wild-type mice; however, no differences in plasma FFA or triglyceride levels are observed   (MGI Ref ID J:50262)
  • insulin resistance
    • after a 6 hr fast, 42-58 week old (obese) male hemizygotes show both hyperinsulinemia and mildly increased blood glucose levels, indicating insulin resistance   (MGI Ref ID J:50262)
  • nervous system phenotype
  • reduced long term potentiation
    • selective impairment of long-term potentiation restricted to medial perforant path-dentate gyrus synapses of mutants, indicating perturbed dentate gyrus function   (MGI Ref ID J:51567)
  • immune system phenotype
  • abnormal tumor necrosis factor level
    • obese hemizygotes display markedly increased white adipose tissue TNF mRNA levels, despite normal plasma FFA and corticosterone levels   (MGI Ref ID J:50262)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Htr2ctm1Jul/Y

        either: (involves: 129S2/SvPas * C57BL/6J * DBA/2) or (involves: 129S4/SvJae * C57BL/6J * DBA/2)
  • mortality/aging
  • premature death
    • male hemizygotes are prone to sudden death from seizures   (MGI Ref ID J:24339)
    • spontaneous deaths are first observed in the fifth week after birth   (MGI Ref ID J:24339)
    • survival rate continues to decline thereafter, such that less than 60% survival is noted at 25 weeks of age   (MGI Ref ID J:24339)
    • however, deaths are not preceded by any obvious health problems   (MGI Ref ID J:24339)
  • growth/size/body phenotype
  • increased body weight
    • male hemizygotes show a 13% increase in average total body mass relative to wild-type controls, with no difference in body length   (MGI Ref ID J:24339)
    • in rare cases, male hemizygotes weigh twice as much as wild-type siblings, attaining a weight of up to 82 g   (MGI Ref ID J:24339)
    • when fed ad libitum, male hemizygotes weigh significantly more than wild-type controls, with no significant changes in body length   (MGI Ref ID J:24339)
    • however, when pair-fed for 4 weeks postweaning and fasted overnight, male hemizygotes that have been provided only the amount of food consumed by their wild-type partners show no differences in body mass, length, white adipose tissue deposits, or plasma glucose and insulin levels relative to wild-type littermates that have been fed ad libitum   (MGI Ref ID J:24339)
  • adipose tissue phenotype
  • increased epididymal fat pad weight
    • male hemizygotes show a >30% increase in epididymal white adipose tissue deposits relative to wild-type controls   (MGI Ref ID J:24339)
  • increased renal fat pad weight
    • male hemizygotes show a 48% increase in perirenal white adipose tissue deposits relative to wild-type controls   (MGI Ref ID J:24339)
  • increased white adipose tissue amount
    • male hemizygotes display significantly larger white adipose tissue deposits than wild-type controls   (MGI Ref ID J:24339)
    • in contrast, brown adipose tissue weights are not significantly altered   (MGI Ref ID J:24339)
  • behavior/neurological phenotype
  • *normal* behavior/neurological phenotype
    • hemizygous males display normal sensitivity to thermal and mechanical nociceptive stimuli relative to wild-type controls   (MGI Ref ID J:24339)
    • impaired behavioral response to xenobiotic
      • o unlike wild-type controls where i.p. admninistration of the serotonergic agonist m-chlorophenylpiperazine (mCPP, 5 mg/kg) reduces food intake by 78%, male hemizygotes are resistant to the appetite suppresant (anorectic) actions of mCPP   (MGI Ref ID J:24339)
    • increased grooming behavior
      • spontaneous seizures are often preceded by repetitive grooming of the snout   (MGI Ref ID J:24339)
    • increased susceptibility to pharmacologically induced seizures
      • in response to i.v. infusion of metrazol (an epileptogenic GABAA receptor antagonist) at a constant rate, 11-15 week old male hemizygotes display a 24% reduction in seizure threshold (onset of first twitch response), an 83% decrease in the duration of the tonic-clonic phase, and a 48% decrease in the lethal dose, relative to wild-type controls   (MGI Ref ID J:24339)
    • polyphagia
      • male hemizygotes display increased food consumption as a result of a behavioral disorder rather than a metabolic defect   (MGI Ref ID J:24339)
    • sporadic seizures
      • male hemizygotes display spontaneous epileptic seizures, with a frequency not greater than 2 or 3 per day   (MGI Ref ID J:24339)
    • tonic seizures
      • occasionally, seizures progress to a tonic extension phase, resulting in respiratory arrest and death within seconds of seizure onset   (MGI Ref ID J:24339)
    • tonic-clonic seizures
      • after the initial repetitive grooming activity, male hemizygotes fall on their sides and progress to hyperkinetic tonic-clonic seizures   (MGI Ref ID J:24339)
      • in most cases, hemizygotes recover quickly and resume a normal behavior; this cycle may be repeated over the next few minutes   (MGI Ref ID J:24339)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • hemizygous males display normal brain cytoarchitecture and ventricular size relative to wild-type controls   (MGI Ref ID J:24339)
    • no differences in hippocampal LTP are observed in the CA1 and CA2 regions relative to wild-type controls   (MGI Ref ID J:24339)
    • increased susceptibility to pharmacologically induced seizures
      • in response to i.v. infusion of metrazol (an epileptogenic GABAA receptor antagonist) at a constant rate, 11-15 week old male hemizygotes display a 24% reduction in seizure threshold (onset of first twitch response), an 83% decrease in the duration of the tonic-clonic phase, and a 48% decrease in the lethal dose, relative to wild-type controls   (MGI Ref ID J:24339)
    • sporadic seizures
      • male hemizygotes display spontaneous epileptic seizures, with a frequency not greater than 2 or 3 per day   (MGI Ref ID J:24339)
    • tonic seizures
      • occasionally, seizures progress to a tonic extension phase, resulting in respiratory arrest and death within seconds of seizure onset   (MGI Ref ID J:24339)
    • tonic-clonic seizures
      • after the initial repetitive grooming activity, male hemizygotes fall on their sides and progress to hyperkinetic tonic-clonic seizures   (MGI Ref ID J:24339)
      • in most cases, hemizygotes recover quickly and resume a normal behavior; this cycle may be repeated over the next few minutes   (MGI Ref ID J:24339)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Receptor Defects
      serotonin

Htr2ctm1Jul related

Diabetes and Obesity Research
Obesity Without Diabetes
      moderate, adult onset

Neurobiology Research
Behavioral and Learning Defects
Epilepsy
Neurotransmitter Receptor and Synaptic Vesicle Defects
Receptor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Htr2ctm1Jul
Allele Name targeted mutation 1, David Julius
Allele Type Targeted (knock-out)
Common Name(s) 5-HT2cR-deficient;
Mutation Made ByDr. David Julius,   Univ of California at San Francisco
Strain of Origin129S/Sv
ES Cell Line NameOther (see notes)
ES Cell Line Strain129
Gene Symbol and Name Htr2c, 5-hydroxytryptamine (serotonin) receptor 2C
Chromosome X
Gene Common Name(s) 5-HT2C; 5-HT2C receptor; 5-HT2cR; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 1C; 5HT-1C; 5HT1c; 5HTR2C; HTR1C; Htr1c; SR1;
General Note ES cell line = D3 (129S2/SvPas) or J1 (129S4/SvJae).
Molecular Note Insertion of a nonsense mutation into exon 5 of the gene resulted in the introduction of a stop codon within the fifth putative transmembrane segment of the receptor and in the elimination of the carboxy-terminal half of the protein. [MGI Ref ID J:24339]

Genotyping

Genotyping Information


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Tecott LH; Sun LM; Akana SF; Strack AM; Lowenstein DH; Dallman MF; Julius D. 1995. Eating disorder and epilepsy in mice lacking 5-HT2c serotonin receptors [see comments] Nature 374(6522):542-6. [PubMed: 7700379]  [MGI Ref ID J:24339]

Additional References

Htr2ctm1Jul related

Abdallah L; Bonasera SJ; Hopf FW; O'Dell L; Giorgetti M; Jongsma M; Carra S; Pierucci M; Di Giovanni G; Esposito E; Parsons LH; Bonci A; Tecott LH. 2009. Impact of serotonin 2C receptor null mutation on physiology and behavior associated with nigrostriatal dopamine pathway function. J Neurosci 29(25):8156-65. [PubMed: 19553455]  [MGI Ref ID J:150413]

Akana SF. 2008. Feeding and stress interact through the serotonin 2C receptor in developing mice. Physiol Behav 94(4):569-79. [PubMed: 18495184]  [MGI Ref ID J:139775]

Applegate CD; Tecott LH. 1998. Global increases in seizure susceptibility in mice lacking 5-HT2C receptors: a behavioral analysis. Exp Neurol 154(2):522-30. [PubMed: 9878187]  [MGI Ref ID J:52051]

Asarian L. 2009. Loss of cholecystokinin and glucagon-like peptide-1-induced satiation in mice lacking serotonin 2C receptors. Am J Physiol Regul Integr Comp Physiol 296(1):R51-6. [PubMed: 19005016]  [MGI Ref ID J:143542]

Brennan TJ; Seeley WW; Kilgard M; Schreiner CE; Tecott LH. 1997. Sound-induced seizures in serotonin 5-HT2c receptor mutant mice. Nat Genet 16(4):387-90. [PubMed: 9241279]  [MGI Ref ID J:42053]

Chou-Green JM; Holscher TD; Dallman MF; Akana SF. 2003. Compulsive behavior in the 5-HT2C receptor knockout mouse. Physiol Behav 78(4-5):641-9. [PubMed: 12782219]  [MGI Ref ID J:96440]

Chou-Green JM; Holscher TD; Dallman MF; Akana SF. 2003. Repeated stress in young and old 5-HT(2C) receptor knockout mice. Physiol Behav 79(2):217-26. [PubMed: 12834793]  [MGI Ref ID J:96453]

Fletcher PJ; Tampakeras M; Sinyard J; Slassi A; Isaac M; Higgins GA. 2009. Characterizing the effects of 5-HT(2C) receptor ligands on motor activity and feeding behaviour in 5-HT(2C) receptor knockout mice. Neuropharmacology 57(3):259-67. [PubMed: 19501602]  [MGI Ref ID J:179499]

Frank MG; Stryker MP; Tecott LH. 2002. Sleep and sleep homeostasis in mice lacking the 5-HT2c receptor. Neuropsychopharmacology 27(5):869-73. [PubMed: 12431861]  [MGI Ref ID J:106164]

Heisler LK; Pronchuk N; Nonogaki K; Zhou L; Raber J; Tung L; Yeo GS; O'Rahilly S; Colmers WF; Elmquist JK; Tecott LH. 2007. Serotonin activates the hypothalamic-pituitary-adrenal axis via serotonin 2C receptor stimulation. J Neurosci 27(26):6956-64. [PubMed: 17596444]  [MGI Ref ID J:122977]

Heisler LK; Tecott LH. 2000. A paradoxical locomotor response in serotonin 5-HT(2C) receptor mutant mice. J Neurosci 20(8):RC71. [PubMed: 10751458]  [MGI Ref ID J:121198]

Heisler LK; Zhou L; Bajwa P; Hsu J; Tecott LH. 2007. Serotonin 5-HT(2C) receptors regulate anxiety-like behavior. Genes Brain Behav 6(5):491-6. [PubMed: 17451451]  [MGI Ref ID J:137308]

Hsu JL; Yu L; Sullivan E; Bowman M; Mistlberger RE; Tecott LH. 2010. Enhanced food anticipatory activity associated with enhanced activation of extrahypothalamic neural pathways in serotonin2C receptor null mutant mice. PLoS One 5(7):e11802. [PubMed: 20668550]  [MGI Ref ID J:163085]

Lin L; York DA. 2005. 5-HT1B receptors modulate the feeding inhibitory effects of enterostatin. Brain Res 1062(1-2):26-31. [PubMed: 16256085]  [MGI Ref ID J:103466]

Lopez-Gimenez JF; Tecott LH; Palacios JM; Mengod G; Vilaro MT. 2002. Serotonin 5- HT (2C) receptor knockout mice: autoradiographic analysis of multiple serotonin receptors. J Neurosci Res 67(1):69-85. [PubMed: 11754082]  [MGI Ref ID J:104818]

Memon RA; Tecott LH; Nonogaki K; Beigneux A; Moser AH; Grunfeld C; Feingold KR. 2000. Up-regulation of peroxisome proliferator-activated receptors (PPAR-alpha) and PPAR-gamma messenger ribonucleic acid expression in the liver in murine obesity: troglitazone induces expression of PPAR-gamma-responsive adipose tissue-specific genes in the liver of obese diabetic mice [In Process Citation] Endocrinology 141(11):4021-31. [PubMed: 11089532]  [MGI Ref ID J:65569]

Mikulski Z; Zaslona Z; Cakarova L; Hartmann P; Wilhelm J; Tecott LH; Lohmeyer J; Kummer W. 2010. Serotonin activates murine alveolar macrophages through 5-HT2C receptors. Am J Physiol Lung Cell Mol Physiol 299(2):L272-80. [PubMed: 20495077]  [MGI Ref ID J:163356]

Nonogaki K; Abdallah L; Goulding EH; Bonasera SJ; Tecott LH. 2003. Hyperactivity and Reduced Energy Cost of Physical Activity in Serotonin 5-HT(2C) Receptor Mutant Mice. Diabetes 52(2):315-20. [PubMed: 12540602]  [MGI Ref ID J:81611]

Nonogaki K; Kaji T; Ohba Y; Sumii M; Wakameda M; Tamari T. 2009. Serotonin 5-HT2C receptor-independent expression of hypothalamic NOR1, a novel modulator of food intake and energy balance, in mice. Biochem Biophys Res Commun 386(2):311-5. [PubMed: 19523439]  [MGI Ref ID J:151527]

Nonogaki K; Memon RA; Grunfeld C; Feingold KR; Tecott LH. 2002. Altered gene expressions involved in energy expenditure in 5-HT(2C) receptor mutant mice. Biochem Biophys Res Commun 295(2):249-54. [PubMed: 12150939]  [MGI Ref ID J:108471]

Nonogaki K; Ohba Y; Sumii M; Oka Y. 2008. Serotonin systems upregulate the expression of hypothalamic NUCB2 via 5-HT2C receptors and induce anorexia via a leptin-independent pathway in mice. Biochem Biophys Res Commun 372(1):186-90. [PubMed: 18477467]  [MGI Ref ID J:136954]

Nonogaki K; Strack AM; Dallman MF; Tecott LH. 1998. Leptin-independent hyperphagia and type 2 diabetes in mice with a mutated serotonin 5-HT2C receptor gene [see comments] Nat Med 4(10):1152-6. [PubMed: 9771748]  [MGI Ref ID J:50262]

Nonogaki K; Suzuki M; Sanuki M; Wakameda M; Tamari T. 2011. The contribution of serotonin 5-HT2C and melanocortin-4 receptors to the satiety signaling of glucagon-like peptide 1 and liragultide, a glucagon-like peptide 1 receptor agonist, in mice. Biochem Biophys Res Commun 411(2):445-8. [PubMed: 21756875]  [MGI Ref ID J:174689]

Rocha BA; Goulding EH; O'Dell LE; Mead AN; Coufal NG; Parsons LH; Tecott LH. 2002. Enhanced locomotor, reinforcing, and neurochemical effects of cocaine in serotonin 5-hydroxytryptamine 2C receptor mutant mice. J Neurosci 22(22):10039-45. [PubMed: 12427861]  [MGI Ref ID J:80185]

Tecott LH; Logue SF; Wehner JM; Kauer JA. 1998. Perturbed dentate gyrus function in serotonin 5-HT2C receptor mutant mice. Proc Natl Acad Sci U S A 95(25):15026-31. [PubMed: 9844009]  [MGI Ref ID J:51567]

Toth M; Tecott L. 1999. Transgenic approaches to epilepsy. Adv Neurol 79:291-6. [PubMed: 10514821]  [MGI Ref ID J:59737]

Vickers SP; Clifton PG; Dourish CT; Tecott LH. 1999. Reduced satiating effect of d-fenfluramine in serotonin 5-HT(2C) receptor mutant mice. Psychopharmacology (Berl) 143(3):309-14. [PubMed: 10353435]  [MGI Ref ID J:57205]

Wade JM; Juneja P; MacKay AW; Graham J; Havel PJ; Tecott LH; Goulding EH. 2008. Synergistic impairment of glucose homeostasis in ob/ob mice lacking functional serotonin 2C receptors. Endocrinology 149(3):955-61. [PubMed: 18039786]  [MGI Ref ID J:135963]

Wang B; Chehab FF. 2006. Deletion of the serotonin 2c receptor from transgenic mice overexpressing leptin does not affect their lipodystrophy but exacerbates their diet-induced obesity. Biochem Biophys Res Commun 351(2):418-23. [PubMed: 17064660]  [MGI Ref ID J:116543]

Yang Y; Mahaffey CL; Berube N; Maddatu TP; Cox GA; Frankel WN. 2007. Complex seizure disorder caused by Brunol4 deficiency in mice. PLoS Genet 3(7):e124. [PubMed: 17677002]  [MGI Ref ID J:123744]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2450.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3185.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

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Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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