Strain Name:

B6;129S4-Nos1tm1Plh/J

Stock Number:

002633

Availability:

Level 4

Description

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered Mutant Mice.
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
GenerationF?+N1F16 (20-DEC-06)
 
Donating Investigator Paul Huang,   Mass General Hospital, Harvard Med Sch

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Nos1tm1Plh targeted mutation are viable and fertile; however, they have enlarged stomachs and reduced stroke size. Hyperglycemic-euglycemic clamp studies demonstrate that homozygotes exhibit insulin resistance at the level of peripheral tissues. Homozygous males are reported to be more aggressive than normal wildtype siblings although this has not been confirmed in The Jackson Laboratory colony. These mice may be useful model for stroke research.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Nos1tm1Plh allele
002986   B6.129S4-Nos1tm1Plh/J
View Strains carrying   Nos1tm1Plh     (1 strain)

Strains carrying other alleles of Nos1
008519   B6.129S4-Nos1tm2Plh/J
View Strains carrying other alleles of Nos1     (1 strain)

Additional Web Information

Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms
Pyloric Stenosis, Infantile Hypertrophic 1; IHPS1 - Models with phenotypic similarity to human disease where etiologies involve orthologs.1
1 Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).
View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Nos1tm1Plh/Nos1tm1Plh

        involves: 129S4/SvJae * C57BL/6
  • life span-post-weaning/aging
  • premature death (MGI Ref ID J:16390)
    • slight increase in premature death
  • digestive/alimentary phenotype
  • abnormal stomach morphology (MGI Ref ID J:16390)
    • hypertrophy of the pyloric sphincter and the circular muscle layer
    • with age, the stomachs lose their interior folds and undergo thinning of the walls, however the intestine appears normal
    • enlarged stomach (MGI Ref ID J:16390)
      • stomach dilation, however architecture of the stomach layers is preserved
    • pyloric stenosis (MGI Ref ID J:16390)
  • reproductive system phenotype
  • *normal* reproductive system phenotype (MGI Ref ID J:89704)
    • mutant males show normal erectile response as measured by maximal intracavernous pressure following papaverine injection unlike Nos3tm1Plh homozygotes
    • abnormal ovulation (MGI Ref ID J:89701)
      • ovulatory efficiency (number oocytes recovered divided by the number of ovarian rupture sites) is reduced in homozygotes
      • significantly fewer oocytes are recovered following gonadotropin-stimulated ovulation in homozygous mutant females compared to wild-type females
  • nervous system phenotype
  • *normal* nervous system phenotype (MGI Ref ID J:16390)
    • homozygous mice do not exhibit defects in brain structure or neuron degeneration

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Nos1tm1Plh/Nos1tm1Plh

        involves: 129S4/SvJae
  • life span-post-weaning/aging
  • decreased survivor rate (MGI Ref ID J:100308)
    • about 80% survival rate at 10 months of age
  • premature death (MGI Ref ID J:100308)
    • about 20% die before 10 months of age
  • homeostasis/metabolism phenotype
  • insulin resistance (MGI Ref ID J:62229)
    • insulin resistance in peripheral tissues

Nos1tm1Plh/Nos1tm1Plh

        B6.129S4-Nos1tm1Plh
  • cardiovascular system phenotype
  • abnormal physiological neovascularization (MGI Ref ID J:106207)
    • retinae show much less neovascularization and significantly less lesions 14 days after production of photocoagulation burns compared to heterozygous littermates
  • choroidal neovascularization (MGI Ref ID J:106207)
    • knockouts show a 58% decrease in neovascularization at sites of Bruch's membrane rupture compared to heterozygous littermates
  • vision/eye phenotype
  • choroidal neovascularization (MGI Ref ID J:106207)
    • knockouts show a 58% decrease in neovascularization at sites of Bruch's membrane rupture compared to heterozygous littermates
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Nos1tm1Plh related

Diabetes and Obesity Research
Insulin Resistance

Neurobiology Research
Neurotransmitter Receptor and Synaptic Vesicle Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol Nos1tm1Plh
Allele Name targeted mutation 1, Paul L Huang
Allele Type Targeted (knock-out)
Common Name(s) Kn; N2KO; NOSdelta; nNOS KO; nNOS-;
Mutation Made By Paul Huang,   Mass General Hospital, Harvard Med Sch
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Nos1, nitric oxide synthase 1, neuronal
Chromosome 5
Gene Common Name(s) 2310005C01Rik; IHPS1; NO; NOS; Nos-1; RIKEN cDNA 2310005C01 gene; bNOS; nNOS; nitric oxide synthase 1 (macrophage);
Molecular Note A neomycin cassette replaced exon 1. This exon encodes the initiation site and amino acids 1 - 159 of the protein. Northern blots of brain homogenates from homozygous mutant mice showed no detectable mRNA. Western blots of brain homogenates from homozygous mutant mice showed no detectable protein. However, splice variants are expressed and variable low levels of NOS activity are seen in the brain. [MGI Ref ID J:16390] [MGI Ref ID J:31846] [MGI Ref ID J:62375]

Genotyping

Genotyping Information

Genotyping Protocols

Nos1tm1Plh, HRM, vers. 2
Nos1tm1Plh, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Huang PL; Dawson TM; Bredt DS; Snyder SH; Fishman MC. 1993. Targeted disruption of the neuronal nitric oxide synthase gene. Cell 75(7):1273-86. [PubMed: 7505721]  [MGI Ref ID J:16390]

Additional References

Bonthius DJ; Tzouras G; Karacay B; Mahoney J; Hutton A; McKim R; Pantazis NJ. 2002. Deficiency of neuronal nitric oxide synthase (nNOS) worsens alcohol-induced microencephaly and neuronal loss in developing mice. Brain Res Dev Brain Res 138(1):45-59. [PubMed: 12234657]  [MGI Ref ID J:79331]

Chen L; Majde JA; Krueger JM. 2003. Spontaneous sleep in mice with targeted disruptions of neuronal or inducible nitric oxide synthase genes. Brain Res 973(2):214-22. [PubMed: 12738065]  [MGI Ref ID J:83571]

Chen L; Toth M. 2001. Fragile X mice develop sensory hyperreactivity to auditory stimuli. Neuroscience 103(4):1043-50. [PubMed: 11301211]  [MGI Ref ID J:85912]

Le Roy I; Pothion S; Mortaud S; Chabert C; Nicolas L; Cherfouh A; Roubertoux PL. 2000. Loss of aggression, after transfer onto a C57BL/6J background, in mice carrying a targeted disruption of the neuronal nitric oxide synthase gene. Behav Genet 30(5):367-73. [PubMed: 11235982]  [MGI Ref ID J:67776]

Nelson RJ; Demas GE; Huang PL; Fishman MC; Dawson VL; Dawson TM; Snyder SH. 1995. Behavioural abnormalities in male mice lacking neuronal nitric oxide synthase [see comments] Nature 378(6555):383-6. [PubMed: 7477374]  [MGI Ref ID J:29970]

Shankar RR; Wu Y; Shen HQ; Zhu JS; Baron AD. 2000. Mice with gene disruption of both endothelial and neuronal nitric oxide synthase exhibit insulin resistance. Diabetes 49(5):684-7. [PubMed: 10905473]  [MGI Ref ID J:62229]

Tranguch S; Huet-Hudson Y. 2003. Decreased viability of nitric oxide synthase double knockout mice. Mol Reprod Dev 65(2):175-9. [PubMed: 12704728]  [MGI Ref ID J:83112]

Nos1tm1Plh related

Andersen YS; Gillin FD; Eckmann L. 2006. Adaptive immunity-dependent intestinal hypermotility contributes to host defense against Giardia spp. Infect Immun 74(4):2473-6. [PubMed: 16552082]  [MGI Ref ID J:107409]

Anderson JE. 2000. A role for nitric oxide in muscle repair: nitric oxide-mediated activation of muscle satellite cells. Mol Biol Cell 11(5):1859-74. [PubMed: 10793157]  [MGI Ref ID J:120497]

Ando A; Yang A; Mori K; Yamada H; Yamada E; Takahashi K; Saikia J; Kim M; Melia M; Fishman M; Huang P; Campochiaro PA. 2002. Nitric oxide is proangiogenic in the retina and choroid. J Cell Physiol 191(1):116-24. [PubMed: 11920687]  [MGI Ref ID J:106207]

Ashley EA; Sears CE; Bryant SM; Watkins HC; Casadei B. 2002. Cardiac nitric oxide synthase 1 regulates basal and beta-adrenergic contractility in murine ventricular myocytes. Circulation 105(25):3011-6. [PubMed: 12081996]  [MGI Ref ID J:103346]

Ayata C; Ayata G; Hara H; Matthews RT; Beal MF; Ferrante RJ ; Endres M ; Kim A ; Christie RH ; Waeber C ; Huang PL ; Hyman BT ; Moskowitz MA. 1997. Mechanisms of reduced striatal NMDA excitotoxicity in type I nitric oxide synthase knock-out mice. J Neurosci 17(18):6908-17. [PubMed: 9278526]  [MGI Ref ID J:42885]

Barouch LA; Cappola TP; Harrison RW; Crone JK; Rodriguez ER; Burnett AL; Hare JM. 2003. Combined loss of neuronal and endothelial nitric oxide synthase causes premature mortality and age-related hypertrophic cardiac remodeling in mice. J Mol Cell Cardiol 35(6):637-44. [PubMed: 12788381]  [MGI Ref ID J:129064]

Barouch LA; Harrison RW; Skaf MW; Rosas GO; Cappola TP; Kobeissi ZA; Hobai IA; Lemmon CA; Burnett AL; O'Rourke B; Rodriguez ER; Huang PL; Lima JA; Berkowitz DE; Hare JM. 2002. Nitric oxide regulates the heart by spatial confinement of nitric oxide synthase isoforms. Nature 416(6878):337-9. [PubMed: 11907582]  [MGI Ref ID J:75645]

Beck PL; Xavier R; Wong J; Ezedi I; Mashimo H; Mizoguchi A; Mizoguchi E; Bhan AK; Podolsky DK. 2004. Paradoxical roles of different nitric oxide synthase isoforms in colonic injury. Am J Physiol Gastrointest Liver Physiol 286(1):G137-47. [PubMed: 14665440]  [MGI Ref ID J:87601]

Bernstein HG; Keilhoff G; Seidel B; Stanarius A; Huang PL; Fishman MC; Reiser M; Bogerts B; Wolf G. 1998. Expression of hypothalamic peptides in mice lacking neuronal nitric oxide synthase: reduced beta-END immunoreactivity in the arcuate nucleus. Neuroendocrinology 68(6):403-11. [PubMed: 9873204]  [MGI Ref ID J:51911]

Bilbo SD; Hotchkiss AK; Chiavegatto S; Nelson RJ. 2003. Blunted stress responses in delayed type hypersensitivity in mice lacking the neuronal isoform of nitric oxide synthase. J Neuroimmunol 140(1-2):41-8. [PubMed: 12864970]  [MGI Ref ID J:119000]

Bonthius DJ; Tzouras G; Karacay B; Mahoney J; Hutton A; McKim R; Pantazis NJ. 2002. Deficiency of neuronal nitric oxide synthase (nNOS) worsens alcohol-induced microencephaly and neuronal loss in developing mice. Brain Res Dev Brain Res 138(1):45-59. [PubMed: 12234657]  [MGI Ref ID J:79331]

Brenman JE; Chao DS; Gee SH; McGee AW; Craven SE; Santillano DR; Wu Z; Huang F; Xia H; Peters MF; Froehner SC; Bredt DS. 1996. Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains. Cell 84(5):757-67. [PubMed: 8625413]  [MGI Ref ID J:31846]

Browne SE; Ayata C; Huang PL; Moskowitz MA; Beal MF. 1999. The cerebral metabolic consequences of nitric oxide synthase deficiency: glucose utilization in endothelial and neuronal nitric oxide synthase null mice. J Cereb Blood Flow Metab 19(2):144-8. [PubMed: 10027769]  [MGI Ref ID J:54728]

Burnett AL; Nelson RJ; Calvin DC; Liu JX; Demas GE; Klein SL; Kriegsfeld LJ; Dawson VL; Dawson TM; Snyder SH. 1996. Nitric oxide-dependent penile erection in mice lacking neuronal nitric oxide synthase. Mol Med 2(3):288-96. [PubMed: 8784782]  [MGI Ref ID J:113071]

Calingasan NY; Huang PL; Chun HS; Fabian A; Gibson GE. 2000. Vascular factors are critical in selective neuronal loss in an animal model of impaired oxidative metabolism. J Neuropathol Exp Neurol 59(3):207-17. [PubMed: 10744059]  [MGI Ref ID J:62409]

Castrop H; Schweda F; Mizel D; Huang Y; Briggs J; Kurtz A; Schnermann J. 2004. Permissive role of nitric oxide in macula densa control of renin secretion. Am J Physiol Renal Physiol 286(5):F848-57. [PubMed: 15075180]  [MGI Ref ID J:113763]

Champion HC; Bivalacqua TJ; Takimoto E; Kass DA; Burnett AL. 2005. Phosphodiesterase-5A dysregulation in penile erectile tissue is a mechanism of priapism. Proc Natl Acad Sci U S A 102(5):1661-6. [PubMed: 15668387]  [MGI Ref ID J:96103]

Chao DS; Silvagno F; Bredt DS. 1998. Muscular dystrophy in mdx mice despite lack of neuronal nitric oxide synthase. J Neurochem 71(2):784-9. [PubMed: 9681470]  [MGI Ref ID J:48851]

Chen G; Dunbar RL; Gao W; Ebner TJ. 2001. Role of calcium, glutamate neurotransmission, and nitric oxide in spreading acidification and depression in the cerebellar cortex. J Neurosci 21(24):9877-87. [PubMed: 11739595]  [MGI Ref ID J:73381]

Chen J; Tu Y; Moon C; Matarazzo V; Palmer AM; Ronnett GV. 2004. The localization of neuronal nitric oxide synthase may influence its role in neuronal precursor proliferation and synaptic maintenance. Dev Biol 269(1):165-82. [PubMed: 15081365]  [MGI Ref ID J:106231]

Chen L; Majde JA; Krueger JM. 2003. Spontaneous sleep in mice with targeted disruptions of neuronal or inducible nitric oxide synthase genes. Brain Res 973(2):214-22. [PubMed: 12738065]  [MGI Ref ID J:83571]

Chen L; Taishi P; Duricka D; Krueger JM. 2004. Brainstem prolactin mRNA is enhanced in mice with suppressed neuronal nitric oxide synthase activity. Brain Res Mol Brain Res 129(1-2):179-84. [PubMed: 15469894]  [MGI Ref ID J:115454]

Chen L; Taishi P; Majde JA; Peterfi Z; Obal F Jr; Krueger JM. 2004. The role of nitric oxide synthases in the sleep responses to tumor necrosis factor-alpha. Brain Behav Immun 18(4):390-8. [PubMed: 15157956]  [MGI Ref ID J:105452]

Chiavegatto S; Dawson VL; Mamounas LA; Koliatsos VE; Dawson TM; Nelson RJ. 2001. Brain serotonin dysfunction accounts for aggression in male mice lacking neuronal nitric oxide synthase. Proc Natl Acad Sci U S A 98(3):1277-81. [PubMed: 11158630]  [MGI Ref ID J:127032]

Choate JK; Danson EJ; Morris JF; Paterson DJ. 2001. Peripheral vagal control of heart rate is impaired in neuronal NOS knockout mice. Am J Physiol Heart Circ Physiol 281(6):H2310-7. [PubMed: 11709397]  [MGI Ref ID J:108674]

Choate JK; Murphy SM; Feldman R; Anderson CR. 2008. Sympathetic control of heart rate in nNOS knockout mice. Am J Physiol Heart Circ Physiol 294(1):H354-61. [PubMed: 17951372]  [MGI Ref ID J:132453]

Chu YC; Guan Y; Skinner J; Raja SN; Johns RA; Tao YX. 2005. Effect of genetic knockout or pharmacologic inhibition of neuronal nitric oxide synthase on complete Freund's adjuvant-induced persistent pain. Pain 119(1-3):113-23. [PubMed: 16297560]  [MGI Ref ID J:106376]

Chung YH; Joo KM; Nam RH; Lee WB; Lee KH; Cha CI. 2004. Region-specific alterations in insulin-like growth factor-I receptor in the central nervous system of nNOS knockout mice. Brain Res 1021(1):132-9. [PubMed: 15328041]  [MGI Ref ID J:107781]

Crosbie RH; Straub V; Yun HY; Lee JC; Rafael JA; Chamberlain JS; Dawson VL; Dawson TM; Campbell KP. 1998. mdx muscle pathology is independent of nNOS perturbation. Hum Mol Genet 7(5):823-9. [PubMed: 9536086]  [MGI Ref ID J:47623]

Danson EJ; Mankia KS; Golding S; Dawson T; Everatt L; Cai S; Channon KM; Paterson DJ. 2004. Impaired regulation of neuronal nitric oxide synthase and heart rate during exercise in mice lacking one nNOS allele. J Physiol 558(Pt 3):963-74. [PubMed: 15155789]  [MGI Ref ID J:105579]

Dawson D; Lygate CA; Zhang MH; Hulbert K; Neubauer S; Casadei B. 2005. nNOS gene deletion exacerbates pathological left ventricular remodeling and functional deterioration after myocardial infarction. Circulation 112(24):3729-37. [PubMed: 16344403]  [MGI Ref ID J:116873]

Demchenko IT; Atochin DN; Boso AE; Astern J; Huang PL; Piantadosi CA. 2003. Oxygen seizure latency and peroxynitrite formation in mice lacking neuronal or endothelial nitric oxide synthases. Neurosci Lett 344(1):53-6. [PubMed: 12781920]  [MGI Ref ID J:107964]

Demchenko IT; Atochin DN; Gutsaeva DR; Godfrey RR; Huang PL; Piantadosi CA; Allen BW. 2008. Contributions of nitric oxide synthase isoforms to pulmonary oxygen toxicity, local vs. mediated effects. Am J Physiol Lung Cell Mol Physiol 294(5):L984-90. [PubMed: 18326824]  [MGI Ref ID J:136632]

DiMagno MJ; Hao Y; Tsunoda Y; Williams JA; Owyang C. 2004. Secretagogue-stimulated pancreatic secretion is differentially regulated by constitutive NOS isoforms in mice. Am J Physiol Gastrointest Liver Physiol 286(3):G428-36. [PubMed: 14551061]  [MGI Ref ID J:95674]

Eliasson MJ; Huang Z; Ferrante RJ; Sasamata M; Molliver ME; Snyder SH; Moskowitz MA. 1999. Neuronal nitric oxide synthase activation and peroxynitrite formation in ischemic stroke linked to neural damage. J Neurosci 19(14):5910-8. [PubMed: 10407030]  [MGI Ref ID J:119782]

Elibol B; Soylemezoglu F; Unal I; Fujii M; Hirt L; Huang PL; Moskowitz MA; Dalkara T. 2001. Nitric oxide is involved in ischemia-induced apoptosis in brain: a study in neuronal nitric oxide synthase null mice. Neuroscience 105(1):79-86. [PubMed: 11483302]  [MGI Ref ID J:126861]

Endres M; Scott G; Namura S; Salzman AL; Huang PL; Moskowitz MA; Szabo C. 1998. Role of peroxynitrite and neuronal nitric oxide synthase in the activation of poly(ADP-ribose) synthetase in a murine model of cerebral ischemia-reperfusion. Neurosci Lett 248(1):41-4. [PubMed: 9665659]  [MGI Ref ID J:107969]

Facchinetti F; Sasaki M; Cutting FB; Zhai P; MacDonald JE; Reif D; Beal MF; Huang PL; Dawson TM; Gurney ME; Dawson VL. 1999. Lack of involvement of neuronal nitric oxide synthase in the pathogenesis of a transgenic mouse model of familial amyotrophic lateral sclerosis. Neuroscience 90(4):1483-92. [PubMed: 10338314]  [MGI Ref ID J:57196]

Fagan KA; Tyler RC; Sato K; Fouty BW; Morris KG Jr; Huang PL; McMurtry IF; Rodman DM. 1999. Relative contributions of endothelial, inducible, and neuronal NOS to tone in the murine pulmonary circulation. Am J Physiol 277(3 Pt 1):L472-8. [PubMed: 10484454]  [MGI Ref ID J:57624]

Finney EM; Shatz CJ. 1998. Establishment of patterned thalamocortical connections does not require nitric oxide synthase. J Neurosci 18(21):8826-38. [PubMed: 9786989]  [MGI Ref ID J:112195]

Fritzen S; Schmitt A; Koth K; Sommer C; Lesch KP; Reif A. 2007. Neuronal nitric oxide synthase (NOS-I) knockout increases the survival rate of neural cells in the hippocampus independently of BDNF. Mol Cell Neurosci 35(2):261-71. [PubMed: 17459722]  [MGI Ref ID J:123224]

Gammie SC; Nelson RJ. 1999. Maternal aggression is reduced in neuronal nitric oxide synthase-deficient mice. J Neurosci 19(18):8027-35. [PubMed: 10479702]  [MGI Ref ID J:57543]

Grange RW; Isotani E; Lau KS; Kamm KE; Huang PL; Stull JT. 2001. Nitric oxide contributes to vascular smooth muscle relaxation in contracting fast-twitch muscles. Physiol Genomics 5(1):35-44. [PubMed: 11161004]  [MGI Ref ID J:124464]

Greenberg SS; Ouyang J; Zhao X; Parrish C; Nelson S; Giles TD. 1999. Effects of ethanol on neutrophil recruitment and lung host defense in nitric oxide synthase I and nitric oxide synthase II knockout mice. Alcohol Clin Exp Res 23(9):1435-45. [PubMed: 10512307]  [MGI Ref ID J:59745]

Gunawardana SC; Rocheleau JV; Head WS; Piston DW. 2006. Mechanisms of time-dependent potentiation of insulin release: involvement of nitric oxide synthase. Diabetes 55(4):1029-33. [PubMed: 16567525]  [MGI Ref ID J:108613]

Hao M; Head WS; Gunawardana SC; Hasty AH; Piston DW. 2007. Direct effect of cholesterol on insulin secretion: a novel mechanism for pancreatic beta-cell dysfunction. Diabetes 56(9):2328-38. [PubMed: 17575085]  [MGI Ref ID J:126584]

Harding P; Sigmon DH; Alfie ME; Huang PL; Fishman MC; Beierwaltes WH; Carretero OA. 1997. Cyclooxygenase-2 mediates increased renal renin content induced by low-sodium diet. Hypertension 29(1 Pt 2):297-302. [PubMed: 9039118]  [MGI Ref ID J:111201]

Huang Z; Huang PL; Panahian N; Dalkara T; Fishman MC; Moskowitz MA. 1994. Effects of cerebral ischemia in mice deficient in neuronal nitric oxide synthase. Science 265(5180):1883-5. [PubMed: 7522345]  [MGI Ref ID J:127062]

Hurt KJ; Musicki B; Palese MA; Crone JK; Becker RE; Moriarity JL; Snyder SH; Burnett AL. 2002. Akt-dependent phosphorylation of endothelial nitric-oxide synthase mediates penile erection. Proc Natl Acad Sci U S A 99(6):4061-6. [PubMed: 11904450]  [MGI Ref ID J:89704]

Hurt KJ; Sezen SF; Champion HC; Crone JK; Palese MA; Huang PL; Sawa A; Luo X; Musicki B; Snyder SH; Burnett AL. 2006. Alternatively spliced neuronal nitric oxide synthase mediates penile erection. Proc Natl Acad Sci U S A 103(9):3440-3. [PubMed: 16488973]  [MGI Ref ID J:107168]

Hussain MB; Hobbs AJ; MacAllister RJ. 1999. Autoregulation of nitric oxide-soluble guanylate cyclase-cyclic GMP signalling in mouse thoracic aorta. Br J Pharmacol 128(5):1082-8. [PubMed: 10556946]  [MGI Ref ID J:59681]

Iijima H; Tulic MK; Duguet A; Shan J; Carbonara P; Hamid Q; Eidelman DH. 2005. NOS 1 is required for allergen-induced expression of NOS 2 in mice. Int Arch Allergy Immunol 138(1):40-50. [PubMed: 16103686]  [MGI Ref ID J:115740]

Imam SZ; Newport GD; Itzhak Y; Cadet JL; Islam F; Slikker W; Ali SF. 2001. Peroxynitrite plays a role in methamphetamine-induced dopaminergic neurotoxicity: evidence from mice lacking neuronal nitric oxide synthase gene or overexpressing copper-zinc superoxide dismutase. J Neurochem 76(3):745-9. [PubMed: 11158245]  [MGI Ref ID J:67775]

Inglis FM; Furia F; Zuckerman KE; Strittmatter SM; Kalb RG. 1998. The role of nitric oxide and NMDA receptors in the development of motor neuron dendrites. J Neurosci 18(24):10493-501. [PubMed: 9852587]  [MGI Ref ID J:111340]

Jing E; Nillni EA; Sanchez VC; Stuart RC; Good DJ. 2004. Deletion of the Nhlh2 transcription factor decreases the levels of the anorexigenic peptides alpha melanocyte-stimulating hormone and thyrotropin-releasing hormone and implicates prohormone convertases I and II in obesity. Endocrinology 145(4):1503-13. [PubMed: 14701669]  [MGI Ref ID J:105580]

Jumrussirikul P; Dinerman J; Dawson TM; Dawson VL; Ekelund U; Georgakopoulos D; Schramm LP; Calkins H; Snyder SH; Hare JM; Berger RD. 1998. Interaction between neuronal nitric oxide synthase and inhibitory G protein activity in heart rate regulation in conscious mice. J Clin Invest 102(7):1279-85. [PubMed: 9769319]  [MGI Ref ID J:115244]

Kanai AJ; Pearce LL; Clemens PR; Birder LA; VanBibber MM; Choi SY; de Groat WC; Peterson J. 2001. Identification of a neuronal nitric oxide synthase in isolated cardiac mitochondria using electrochemical detection. Proc Natl Acad Sci U S A 98(24):14126-31. [PubMed: 11717466]  [MGI Ref ID J:125463]

Kano T; Shimizu-Sasamata M; Huang PL; Moskowitz MA; Lo EH. 1998. Effects of nitric oxide synthase gene knockout on neurotransmitter release in vivo. Neuroscience 86(3):695-9. [PubMed: 9692709]  [MGI Ref ID J:119052]

Karacay B; Li G; Pantazis NJ; Bonthius DJ. 2007. Stimulation of the cAMP pathway protects cultured cerebellar granule neurons against alcohol-induced cell death by activating the neuronal nitric oxide synthase (nNOS) gene. Brain Res 1143:34-45. [PubMed: 17306238]  [MGI Ref ID J:121468]

Katoh A; Kitazawa H; Itohara S; Nagao S. 2000. Inhibition of nitric oxide synthesis and gene knockout of neuronal nitric oxide synthase impaired adaptation of mouse optokinetic response eye movements. Learn Mem 7(4):220-6. [PubMed: 10940322]  [MGI Ref ID J:103910]

Keilhoff G; Fansa H; Wolf G. 2002. Differences in peripheral nerve degeneration/regeneration between wild-type and neuronal nitric oxide synthase knockout mice. J Neurosci Res 68(4):432-41. [PubMed: 11992469]  [MGI Ref ID J:104916]

Keilhoff G; Seidel B; Reiser M; Stanarius A; Huang PL; Bogerts B; Wolf G; Bernstein HG. 2001. Lack of neuronal NOS has consequences for the expression of POMC and POMC-derived peptides in the mouse pituitary. Acta Histochem 103(4):397-412. [PubMed: 11700945]  [MGI Ref ID J:102572]

Keilhoff G; Wolf G; Bernstein H. 2001. Altered laminar distribution of hippocampal zinc in mutant mice lacking neuronal nitric oxide synthase. A histochemical study. Neurosci Lett 305(3):173-6. [PubMed: 11403933]  [MGI Ref ID J:108030]

Kim CD; Goyal RK; Mashimo H. 1999. Neuronal NOS provides nitrergic inhibitory neurotransmitter in mouse lower esophageal sphincter. Am J Physiol 277(2 Pt 1):G280-4. [PubMed: 10444441]  [MGI Ref ID J:56948]

Kim MJ; Chung YH; Joo KM; Oh GT; Kim J; Lee B; Cha CI. 2004. Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum. Neurosci Lett 369(1):39-43. [PubMed: 15380304]  [MGI Ref ID J:120019]

Kim MJ; Joo KM; Chung YH; Lee YJ; Kim J; Lee BH; Shin DH; Lee KH; Cha CI. 2003. Vasoactive intestinal peptide (VIP) and VIP mRNA decrease in the cerebral cortex of nNOS knock-out(-/-) mice. Brain Res 978(1-2):233-40. [PubMed: 12834919]  [MGI Ref ID J:86456]

Kinugawa S; Huang H; Wang Z; Kaminski PM; Wolin MS; Hintze TH. 2005. A defect of neuronal nitric oxide synthase increases xanthine oxidase-derived superoxide anion and attenuates the control of myocardial oxygen consumption by nitric oxide derived from endothelial nitric oxide synthase. Circ Res 96(3):355-62. [PubMed: 15637297]  [MGI Ref ID J:106887]

Kirchner L; Weitzdoerfer R; Hoeger H; Url A; Schmidt P; Engelmann M; Villar SR; Fountoulakis M; Lubec G; Lubec B. 2004. Impaired cognitive performance in neuronal nitric oxide synthase knockout mice is associated with hippocampal protein derangements. Nitric Oxide 11(4):316-30. [PubMed: 15604044]  [MGI Ref ID J:101988]

Kitaura H; Uozumi N; Tohmi M; Yamazaki M; Sakimura K; Kudoh M; Shimizu T; Shibuki K. 2007. Roles of nitric oxide as a vasodilator in neurovascular coupling of mouse somatosensory cortex. Neurosci Res 59(2):160-71. [PubMed: 17655958]  [MGI Ref ID J:136589]

Klein SL; Carnovale D; Burnett AL; Wallach EE; Zacur HA; Crone JK; Dawson VL; Nelson RJ; Dawson TM. 1998. Impaired ovulation in mice with targeted deletion of the neuronal isoform of nitric oxide synthase. Mol Med 4(10):658-64. [PubMed: 9848082]  [MGI Ref ID J:89701]

Kline DD; Overholt JL; Prabhakar NR. 2002. Mutant mice deficient in NOS-1 exhibit attenuated long-term facilitation and short-term potentiation in breathing. J Physiol 539(Pt 1):309-15. [PubMed: 11850522]  [MGI Ref ID J:105969]

Kline DD; Yang T; Huang PL; Prabhakar NR. 1998. Altered respiratory responses to hypoxia in mutant mice deficient in neuronal nitric oxide synthase. J Physiol 511(Pt 1):273-87. [PubMed: 9679181]  [MGI Ref ID J:106328]

Koliatsos VE; Dawson TM; Kecojevic A; Zhou Y; Wang YF; Huang KX. 2004. Cortical interneurons become activated by deafferentation and instruct the apoptosis of pyramidal neurons. Proc Natl Acad Sci U S A 101(39):14264-9. [PubMed: 15381772]  [MGI Ref ID J:93439]

Kozak W; Kozak A. 2003. Genetic Models in Applied Physiology. Differential role of nitric oxide synthase isoforms in fever of different etiologies: studies using Nos gene-deficient mice. J Appl Physiol 94(6):2534-44. [PubMed: 12562678]  [MGI Ref ID J:103018]

Kriegsfeld LJ; Eliasson MJ; Demas GE; Blackshaw S; Dawson TM; Nelson RJ; Snyder SH. 1999. Nocturnal motor coordination deficits in neuronal nitric oxide synthase knock-out mice. Neuroscience 89(2):311-5. [PubMed: 10077313]  [MGI Ref ID J:56005]

Lau KS; Grange RW; Isotani E; Sarelius IH; Kamm KE; Huang PL; Stull JT. 2000. nNOS and eNOS modulate cGMP formation and vascular response in contracting fast-twitch skeletal muscle Physiol Genomics 2(1):21-27. [PubMed: 11015578]  [MGI Ref ID J:62742]

Le Roy I; Pothion S; Mortaud S; Chabert C; Nicolas L; Cherfouh A; Roubertoux PL. 2000. Loss of aggression, after transfer onto a C57BL/6J background, in mice carrying a targeted disruption of the neuronal nitric oxide synthase gene. Behav Genet 30(5):367-73. [PubMed: 11235982]  [MGI Ref ID J:67776]

Lefer DJ; Jones SP; Girod WG; Baines A; Grisham MB; Cockrell AS; Huang PL; Scalia R. 1999. Leukocyte-endothelial cell interactions in nitric oxide synthase-deficient mice. Am J Physiol 276(6 Pt 2):H1943-50. [PubMed: 10362674]  [MGI Ref ID J:55936]

Lev-Ram V; Nebyelul Z; Ellisman MH; Huang PL; Tsien RY. 1997. Absence of cerebellar long-term depression in mice lacking neuronal nitric oxide synthase. Learn Mem 4(1):169-177. [PubMed: 10456061]  [MGI Ref ID J:42548]

Levine DZ; Iacovitti M. 2006. Real-time measurement of kidney tubule fluid nitric oxide concentrations in early diabetes: disparate changes in different rodent models. Nitric Oxide 15(1):87-92. [PubMed: 16510300]  [MGI Ref ID J:112767]

Li E; Zhou P; Singer SM. 2006. Neuronal nitric oxide synthase is necessary for elimination of Giardia lamblia infections in mice. J Immunol 176(1):516-21. [PubMed: 16365445]  [MGI Ref ID J:126256]

Li X; Clark JD. 2001. Spinal cord nitric oxide synthase and heme oxygenase limit morphine induced analgesia. Brain Res Mol Brain Res 95(1-2):96-102. [PubMed: 11687280]  [MGI Ref ID J:72814]

Li X; Nemoto M; Xu Z; Yu SW; Shimoji M; Andrabi SA; Haince JF; Poirier GG; Dawson TM; Dawson VL; Koehler RC. 2007. Influence of duration of focal cerebral ischemia and neuronal nitric oxide synthase on translocation of apoptosis-inducing factor to the nucleus. Neuroscience 144(1):56-65. [PubMed: 17049179]  [MGI Ref ID J:117941]

Lidington D; Li F; Tyml K. 2007. Deletion of neuronal NOS prevents impaired vasodilation in septic mouse skeletal muscle. Cardiovasc Res 74(1):151-8. [PubMed: 17258180]  [MGI Ref ID J:119500]

Liu X; Li C; Falck JR; Roman RJ; Harder DR; Koehler RC. 2008. Interaction of nitric oxide, 20-HETE, and EETs during functional hyperemia in whisker barrel cortex. Am J Physiol Heart Circ Physiol 295(2):H619-31. [PubMed: 18502903]  [MGI Ref ID J:138206]

Ma J; Ayata C; Huang PL; Fishman MC; Moskowitz MA. 1996. Regional cerebral blood flow response to vibrissal stimulation in mice lacking type I NOS gene expression. Am J Physiol 270(3 Pt 2):H1085-90. [PubMed: 8780207]  [MGI Ref ID J:113045]

Mang CF; Truempler S; Erbelding D; Kilbinger H. 2002. Modulation by NO of acetylcholine release in the ileum of wild-type and NOS gene knockout mice. Am J Physiol Gastrointest Liver Physiol 283(5):G1132-8. [PubMed: 12381527]  [MGI Ref ID J:108055]

Martin LJ; Chen K; Liu Z. 2005. Adult motor neuron apoptosis is mediated by nitric oxide and Fas death receptor linked by DNA damage and p53 activation. J Neurosci 25(27):6449-59. [PubMed: 16000635]  [MGI Ref ID J:99428]

Martin SR; Emanuel K; Sears CE; Zhang YH; Casadei B. 2006. Are myocardial eNOS and nNOS involved in the beta-adrenergic and muscarinic regulation of inotropy? A systematic investigation. Cardiovasc Res 70(1):97-106. [PubMed: 16545353]  [MGI Ref ID J:107830]

Mashimo H; Kjellin A; Goyal RK. 2000. Gastric stasis in neuronal nitric oxide synthase-deficient knockout mice Gastroenterology 119(3):766-73. [PubMed: 10982771]  [MGI Ref ID J:64225]

Matthews RT; Beal MF; Fallon J; Fedorchak K; Huang PL; Fishman MC; Hyman BT. 1997. MPP+ induced substantia nigra degeneration is attenuated in nNOS knockout mice. Neurobiol Dis 4(2):114-21. [PubMed: 9331901]  [MGI Ref ID J:43295]

Mattson DL; Meister CJ. 2005. Renal cortical and medullary blood flow responses to L-NAME and ANG II in wild-type, nNOS null mutant, and eNOS null mutant mice. Am J Physiol Regul Integr Comp Physiol 289(4):R991-7. [PubMed: 15961532]  [MGI Ref ID J:101244]

McCullough LD; Zeng Z; Blizzard KK; Debchoudhury I; Hurn PD. 2005. Ischemic nitric oxide and poly (ADP-ribose) polymerase-1 in cerebral ischemia: male toxicity, female protection. J Cereb Blood Flow Metab 25(4):502-12. [PubMed: 15689952]  [MGI Ref ID J:112492]

McKinnon RL; Lidington D; Bolon M; Ouellette Y; Kidder GM; Tyml K. 2006. Reduced arteriolar conducted vasoconstriction in septic mouse cremaster muscle is mediated by nNOS-derived NO. Cardiovasc Res 69(1):236-44. [PubMed: 16226732]  [MGI Ref ID J:112791]

Moraes JC; Amaral ME; Picardi PK; Calegari VC; Romanatto T; Bermudez-Echeverry M; Chiavegatto S; Saad MJ; Velloso LA. 2006. Inducible-NOS but not neuronal-NOS participate in the acute effect of TNF-alpha on hypothalamic insulin-dependent inhibition of food intake. FEBS Lett 580(19):4625-31. [PubMed: 16876161]  [MGI Ref ID J:112153]

Morishita T; Tsutsui M; Shimokawa H; Horiuchi M; Tanimoto A; Suda O; Tasaki H; Huang PL; Sasaguri Y; Yanagihara N; Nakashima Y. 2002. Vasculoprotective roles of neuronal nitric oxide synthase. FASEB J 16(14):1994-6. [PubMed: 12397095]  [MGI Ref ID J:120009]

Morishita T; Tsutsui M; Shimokawa H; Sabanai K; Tasaki H; Suda O; Nakata S; Tanimoto A; Wang KY; Ueta Y; Sasaguri Y; Nakashima Y; Yanagihara N. 2005. Nephrogenic diabetes insipidus in mice lacking all nitric oxide synthase isoforms. Proc Natl Acad Sci U S A 102(30):10616-21. [PubMed: 16024729]  [MGI Ref ID J:100308]

Naghashpour M; Dahl G. 2000. Relaxation of myometrium by calcitonin gene-related peptide is independent of nitric oxide synthase activity in mouse uterus. Biol Reprod 63(5):1421-7. [PubMed: 11058547]  [MGI Ref ID J:108667]

Nangle MR; Cotter MA; Cameron NE. 2003. An in vitro study of corpus cavernosum and aorta from mice lacking the inducible nitric oxide synthase gene. Nitric Oxide 9(4):194-200. [PubMed: 14996426]  [MGI Ref ID J:119059]

Nelson RJ; Demas GE; Huang PL; Fishman MC; Dawson VL; Dawson TM; Snyder SH. 1995. Behavioural abnormalities in male mice lacking neuronal nitric oxide synthase [see comments] Nature 378(6555):383-6. [PubMed: 7477374]  [MGI Ref ID J:29970]

Noh HS; Kim DW; Cho GJ; Choi WS; Kang SS. 2006. Increased nitric oxide caused by the ketogenic diet reduces the onset time of kainic acid-induced seizures in ICR mice. Brain Res 1075(1):193-200. [PubMed: 16460714]  [MGI Ref ID J:107300]

Nott A; Watson PM; Robinson JD; Crepaldi L; Riccio A. 2008. S-Nitrosylation of histone deacetylase 2 induces chromatin remodelling in neurons. Nature 455(7211):411-5. [PubMed: 18754010]  [MGI Ref ID J:140120]

O'Dell TJ; Huang PL; Dawson TM; Dinerman JL; Snyder SH; Kandel ER; Fishman MC. 1994. Endothelial NOS and the blockade of LTP by NOS inhibitors in mice lacking neuronal NOS. Science 265(5171):542-6. [PubMed: 7518615]  [MGI Ref ID J:19282]

O'Riordan E; Mendelev N; Patschan S; Patschan D; Eskander J; Cohen-Gould L; Chander P; Goligorsky MS. 2007. Chronic NOS inhibition actuates endothelial-mesenchymal transformation. Am J Physiol Heart Circ Physiol 292(1):H285-94. [PubMed: 16963618]  [MGI Ref ID J:119956]

Paliege A; Mizel D; Medina C; Pasumarthy A; Huang YG; Bachmann S; Briggs JP; Schnermann JB; Yang T. 2004. Inhibition of nNOS expression in the macula densa by COX-2-derived prostaglandin E(2). Am J Physiol Renal Physiol 287(1):F152-9. [PubMed: 15010356]  [MGI Ref ID J:113758]

Phillipson M; Henriksnas J; Holstad M; Sandler S; Holm L. 2003. Inducible nitric oxide synthase is involved in acid-induced gastric hyperemia in rats and mice. Am J Physiol Gastrointest Liver Physiol 285(1):G154-62. [PubMed: 12646421]  [MGI Ref ID J:84264]

Putzke J; Seidel B; Huang PL; Wolf G. 2000. Differential expression of alternatively spliced isoforms of neuronal nitric oxide synthase (nNOS) and N-methyl-D-aspartate receptors (NMDAR) in knockout mice deficient in nNOS alpha (nNOS alpha(Delta/Delta) mice). Brain Res Mol Brain Res 85(1-2):13-23. [PubMed: 11146102]  [MGI Ref ID J:66968]

Rattan S; Regan RF; Patel CA; De Godoy MA. 2005. Nitric oxide not carbon monoxide mediates nonadrenergic noncholinergic relaxation in the murine internal anal sphincter. Gastroenterology 129(6):1954-66. [PubMed: 16344064]  [MGI Ref ID J:124936]

Rothe F; Huang PL; Wolf G. 1999. Ultrastructural localization of neuronal nitric oxide synthase in the laterodorsal tegmental nucleus of wild-type and knockout mice. Neuroscience 94(1):193-201. [PubMed: 10613509]  [MGI Ref ID J:59780]

Rudkowski JC; Barreiro E; Harfouche R; Goldberg P; Kishta O; D'Orleans-Juste P; Labonte J; Lesur O; Hussain SN. 2004. Roles of iNOS and nNOS in sepsis-induced pulmonary apoptosis. Am J Physiol Lung Cell Mol Physiol 286(4):L793-800. [PubMed: 14660484]  [MGI Ref ID J:108149]

Salchner P; Lubec G; Engelmann M; Orlando GF; Wolf G; Sartori SB; Hoeger H; Singewald N. 2004. Genetic functional inactivation of neuronal nitric oxide synthase affects stress-related Fos expression in specific brain regions. Cell Mol Life Sci 61(12):1498-506. [PubMed: 15197473]  [MGI Ref ID J:115481]

Sampei K; Mandir AS; Asano Y; Wong PC; Traystman RJ; Dawson VL; Dawson TM; Hurn PD. 2000. Stroke outcome in double-mutant antioxidant transgenic mice. Stroke 31(11):2685-91. [PubMed: 11062295]  [MGI Ref ID J:128835]

Saraiva RM; Minhas KM; Raju SV; Barouch LA; Pitz E; Schuleri KH; Vandegaer K; Li D; Hare JM. 2005. Deficiency of neuronal nitric oxide synthase increases mortality and cardiac remodeling after myocardial infarction: role of nitroso-redox equilibrium. Circulation 112(22):3415-22. [PubMed: 16301341]  [MGI Ref ID J:116907]

Scheiner CA; Cork RJ; Mize RR. 1999. Failure to disrupt development of cholinergic fiber patches in the superior colliculus in nitric oxide synthase deficient mice. Brain Res Dev Brain Res 118(1-2):217-20. [PubMed: 10611522]  [MGI Ref ID J:59193]

Schild L; Dombrowski F; Lendeckel U; Schulz C; Gardemann A; Keilhoff G. 2008. Impairment of endothelial nitric oxide synthase causes abnormal fat and glycogen deposition in liver. Biochim Biophys Acta 1782(3):180-7. [PubMed: 18206129]  [MGI Ref ID J:133437]

Schild L; Jaroscakova I; Lendeckel U; Wolf G; Keilhoff G. 2006. Neuronal nitric oxide synthase controls enzyme activity pattern of mitochondria and lipid metabolism. FASEB J 20(1):145-7. [PubMed: 16246868]  [MGI Ref ID J:104558]

Schnermann J. 1999. Micropuncture analysis of tubuloglomerular feedback regulation in transgenic mice. J Am Soc Nephrol 10(12):2614-9. [PubMed: 10589702]  [MGI Ref ID J:59838]

Schuh K; Quaschning T; Knauer S; Hu K; Kocak S; Roethlein N; Neyses L. 2003. Regulation of vascular tone in animals overexpressing the sarcolemmal calcium pump. J Biol Chem 278(42):41246-52. [PubMed: 12900399]  [MGI Ref ID J:119413]

Schulz JB; Huang PL; Matthews RT; Passov D; Fishman MC; Beal MF. 1996. Striatal malonate lesions are attenuated in neuronal nitric oxide synthase knockout mice. J Neurochem 67(1):430-3. [PubMed: 8667023]  [MGI Ref ID J:111644]

Sears CE; Bryant SM; Ashley EA; Lygate CA; Rakovic S; Wallis HL; Neubauer S; Terrar DA; Casadei B. 2003. Cardiac neuronal nitric oxide synthase isoform regulates myocardial contraction and calcium handling. Circ Res 92(5):e52-9. [PubMed: 12623875]  [MGI Ref ID J:109339]

Shankar RR; Wu Y; Shen HQ; Zhu JS; Baron AD. 2000. Mice with gene disruption of both endothelial and neuronal nitric oxide synthase exhibit insulin resistance. Diabetes 49(5):684-7. [PubMed: 10905473]  [MGI Ref ID J:62229]

Shesely EG; Gilbert C; Granderson G; Carretero CD; Carretero OA; Beierwaltes WH. 2001. Nitric oxide synthase gene knockout mice do not become hypertensive during pregnancy. Am J Obstet Gynecol 185(5):1198-203. [PubMed: 11717657]  [MGI Ref ID J:117212]

Shiao T; Fond A; Deng B; Wehling-Henricks M; Adams ME; Froehner SC; Tidball JG. 2004. Defects in neuromuscular junction structure in dystrophic muscle are corrected by expression of a NOS transgene in dystrophin-deficient muscles, but not in muscles lacking {alpha}- and {beta}1-syntrophins. Hum Mol Genet 13(17):1873-1884. [PubMed: 15238508]  [MGI Ref ID J:92094]

Shimizu-Sasamata M; Bosque-Hamilton P; Huang PL; Moskowitz MA; Lo EH. 1998. Attenuated neurotransmitter release and spreading depression-like depolarizations after focal ischemia in mutant mice with disrupted type I nitric oxide synthase gene. J Neurosci 18(22):9564-71. [PubMed: 9801393]  [MGI Ref ID J:50898]

Shutoh F; Ohki M; Kitazawa H; Itohara S; Nagao S. 2006. Memory trace of motor learning shifts transsynaptically from cerebellar cortex to nuclei for consolidation. Neuroscience 139(2):767-777. [PubMed: 16458438]  [MGI Ref ID J:107819]

Sivarao DV; Mashimo HL; Thatte HS; Goyal RK. 2001. Lower esophageal sphincter is achalasic in nNOS(-/-) and hypotensive in W/W(v) mutant mice. Gastroenterology 121(1):34-42. [PubMed: 11438492]  [MGI Ref ID J:70182]

Son H; Hawkins RD; Martin K; Kiebler M; Huang PL; Fishman MC; Kandel ER. 1996. Long-term potentiation is reduced in mice that are doubly mutant in endothelial and neuronal nitric oxide synthase. Cell 87(6):1015-23. [PubMed: 8978606]  [MGI Ref ID J:37956]

Spanagel R; Siegmund S; Cowen M; Schroff KC; Schumann G; Fiserova M; Sillaber I; Wellek S; Singer M; Putzke J. 2002. The neuronal nitric oxide synthase gene is critically involved in neurobehavioral effects of alcohol. J Neurosci 22(19):8676-83. [PubMed: 12351742]  [MGI Ref ID J:79215]

Steudel W; Kirmse M; Weimann J; Ullrich R; Hromi J; Zapol WM. 2000. Exhaled nitric oxide production by nitric oxide synthase-deficient mice. Am J Respir Crit Care Med 162(4 Pt 1):1262-7. [PubMed: 11029328]  [MGI Ref ID J:103210]

Sumeray MS; Rees DD; Yellon DM. 2000. Infarct size and nitric oxide synthase in murine myocardium J Mol Cell Cardiol 32(1):35-42. [PubMed: 10652188]  [MGI Ref ID J:60525]

Sun J; Picht E; Ginsburg KS; Bers DM; Steenbergen C; Murphy E. 2006. Hypercontractile female hearts exhibit increased S-nitrosylation of the L-type Ca2+ channel alpha1 subunit and reduced ischemia/reperfusion injury. Circ Res 98(3):403-11. [PubMed: 16397145]  [MGI Ref ID J:118890]

Sun Y; Jin K; Childs JT; Xie L; Mao XO; Greenberg DA. 2005. Neuronal nitric oxide synthase and ischemia-induced neurogenesis. J Cereb Blood Flow Metab 25(4):485-92. [PubMed: 15689958]  [MGI Ref ID J:112491]

Suzuki N; Motohashi N; Uezumi A; Fukada S; Yoshimura T; Itoyama Y; Aoki M; Miyagoe-Suzuki Y; Takeda S. 2007. NO production results in suspension-induced muscle atrophy through dislocation of neuronal NOS. J Clin Invest 117(9):2468-76. [PubMed: 17786240]  [MGI Ref ID J:127415]

Szabadits E; Cserep C; Ludanyi A; Katona I; Gracia-Llanes J; Freund TF; Nyiri G. 2007. Hippocampal GABAergic synapses possess the molecular machinery for retrograde nitric oxide signaling. J Neurosci 27(30):8101-11. [PubMed: 17652601]  [MGI Ref ID J:123246]

Takaki A; Morikawa K; Tsutsui M; Murayama Y; Tekes E; Yamagishi H; Ohashi J; Yada T; Yanagihara N; Shimokawa H. 2008. Crucial role of nitric oxide synthases system in endothelium-dependent hyperpolarization in mice. J Exp Med 205(9):2053-63. [PubMed: 18695006]  [MGI Ref ID J:140478]

Tao F; Tao YX; Zhao C; Dore S; Liaw WJ; Raja SN; Johns RA. 2004. Differential roles of neuronal and endothelial nitric oxide synthases during carrageenan-induced inflammatory hyperalgesia. Neuroscience 128(2):421-30. [PubMed: 15350652]  [MGI Ref ID J:93612]

TeKippe M; Harrison DE; Chen J. 2003. Expansion of hematopoietic stem cell phenotype and activity in Trp53-null mice. Exp Hematol 31(6):521-7. [PubMed: 12829028]  [MGI Ref ID J:115677]

Terauchi A; Kobayashi D; Mashimo H. 2005. Distinct roles of nitric oxide synthases and interstitial cells of Cajal in rectoanal relaxation. Am J Physiol Gastrointest Liver Physiol 289(2):G291-9. [PubMed: 15845873]  [MGI Ref ID J:100350]

Tranguch S; Huet-Hudson Y. 2003. Decreased viability of nitric oxide synthase double knockout mice. Mol Reprod Dev 65(2):175-9. [PubMed: 12704728]  [MGI Ref ID J:83112]

Vallance BA; Dijkstra G; Qiu B; van der Waaij LA; van Goor H; Jansen PL; Mashimo H; Collins SM. 2004. Relative contributions of NOS isoforms during experimental colitis: endothelial-derived NOS maintains mucosal integrity. Am J Physiol Gastrointest Liver Physiol 287(4):G865-74. [PubMed: 15217783]  [MGI Ref ID J:96074]

Wagner C; Godecke A; Ford M; Schnermann J; Schrader J; Kurtz A. 2000. Regulation of renin gene expression in kidneys of eNOS- and nNOS-deficient mice Pflugers Arch 439(5):567-72. [PubMed: 10764216]  [MGI Ref ID J:62375]

Wang H; Kohr MJ; Traynham CJ; Wheeler DG; Janssen PM; Ziolo MT. 2008. Neuronal nitric oxide synthase signaling within cardiac myocytes targets phospholamban. Am J Physiol Cell Physiol 294(6):C1566-75. [PubMed: 18400986]  [MGI Ref ID J:136639]

Wang T; Inglis FM; Kalb RG. 2000. Defective fluid and HCO(3)(-) absorption in proximal tubule of neuronal nitric oxide synthase-knockout mice. Am J Physiol Renal Physiol 279(3):F518-24. [PubMed: 10966931]  [MGI Ref ID J:64896]

Watkins CC; Boehning D; Kaplin AI; Rao M; Ferris CD; Snyder SH. 2004. Carbon monoxide mediates vasoactive intestinal polypeptide-associated nonadrenergic/noncholinergic neurotransmission. Proc Natl Acad Sci U S A 101(8):2631-5. [PubMed: 14983060]  [MGI Ref ID J:88640]

Watkins CC; Sawa A; Jaffrey S; Blackshaw S; Barrow RK; Snyder SH; Ferris CD. 2000. Insulin restores neuronal nitric oxide synthase expression and function that is lost in diabetic gastropathy. J Clin Invest 106(3):373-84. [PubMed: 10930440]  [MGI Ref ID J:63749]

Weitzdoerfer R; Hoeger H; Engidawork E; Engelmann M; Singewald N; Lubec G; Lubec B. 2004. Neuronal nitric oxide synthase knock-out mice show impaired cognitive performance. Nitric Oxide 10(3):130-40. [PubMed: 15158692]  [MGI Ref ID J:101815]

Wu HH; Cork RJ; Huang PL; Shuman DL; Mize RR. 2000. Refinement of the ipsilateral retinocollicular projection is disrupted in double endothelial and neuronal nitric oxide synthase gene knockout mice. Brain Res Dev Brain Res 120(1):105-11. [PubMed: 10727738]  [MGI Ref ID J:61144]

Wu HH; Cork RJ; Mize RR. 2000. Normal development of the ipsilateral retinocollicular pathway and its disruption in double endothelial and neuronal nitric oxide synthase gene knockout mice J Comp Neurol 426(4):651-65. [PubMed: 11027405]  [MGI Ref ID J:64999]

Xu L; Okuda-Ashitaka E; Matsumura S; Mabuchi T; Okamoto S; Sakimura K; Mishina M; Ito S. 2007. Signal pathways coupled to activation of neuronal nitric oxide synthase in the spinal cord by nociceptin/orphanin FQ. Neuropharmacology 52(5):1318-25. [PubMed: 17350656]  [MGI Ref ID J:122321]

Xue L; Farrugia G; Miller SM; Ferris CD; Snyder SH; Szurszewski JH. 2000. Carbon monoxide and nitric oxide as coneurotransmitters in the enteric nervous system: evidence from genomic deletion of biosynthetic enzymes. Proc Natl Acad Sci U S A 97(4):1851-5. [PubMed: 10677545]  [MGI Ref ID J:89581]

Yang G; Zhang Y; Ross ME; Iadecola C. 2003. Attenuation of activity-induced increases in cerebellar blood flow in mice lacking neuronal nitric oxide synthase. Am J Physiol Heart Circ Physiol 285(1):H298-304. [PubMed: 12623792]  [MGI Ref ID J:84255]

Yang JZ; Ajonuma LC; Rowlands DK; Tsang LL; Ho LS; Lam SY; Chen WY; Zhou CX; Chung YW; Cho CY; Tse JY; James AE; Chan HC. 2005. The role of inducible nitric oxide synthase in gamete interaction and fertilization: a comparative study on knockout mice of three NOS isoforms. Cell Biol Int 29(9):785-91. [PubMed: 16087361]  [MGI Ref ID J:112824]

Zaharchuk G; Hara H; Huang PL; Fishman MC; Moskowitz MA; Jenkins BG ; Rosen BR. 1997. Neuronal nitric oxide synthase mutant mice show smaller infarcts and attenuated apparent diffusion coefficient changes in the peri-infarct zone during focal cerebral ischemia. Magn Reson Med 37(2):170-5. [PubMed: 9001139]  [MGI Ref ID J:40122]

Zakhary R; Poss KD; Jaffrey SR; Ferris CD; Tonegawa S; Snyder SH. 1997. Targeted gene deletion of heme oxygenase 2 reveals neural role for carbon monoxide. Proc Natl Acad Sci U S A 94(26):14848-53. [PubMed: 9405702]  [MGI Ref ID J:89562]

Zhu XJ; Hua Y; Jiang J; Zhou QG; Luo CX; Han X; Lu YM; Zhu DY. 2006. Neuronal nitric oxide synthase-derived nitric oxide inhibits neurogenesis in the adult dentate gyrus by down-regulating cyclic AMP response element binding protein phosphorylation. Neuroscience 141(2):827-36. [PubMed: 16735094]  [MGI Ref ID J:111759]

Zhu Y; Ohlemiller KK; McMahan BK; Park TS; Gidday JM. 2006. Constitutive nitric oxide synthase activity is required to trigger ischemic tolerance in mouse retina. Exp Eye Res 82(1):153-63. [PubMed: 16045907]  [MGI Ref ID J:106447]

Zhuang W; Eby JC; Cheong M; Mohapatra PK; Bredt DS; Disatnik MH; Rando TA. 2001. The susceptibility of muscle cells to oxidative stress is independent of nitric oxide synthase expression. Muscle Nerve 24(4):502-11. [PubMed: 11268022]  [MGI Ref ID J:116198]

de Jonge WJ; Hallemeesch MM; Kwikkers KL; Ruijter JM; de Gier-de Vries C; van Roon MA; Meijer AJ; Marescau B; de Deyn PP; Deutz NE; Lamers WH. 2002. Overexpression of arginase I in enterocytes of transgenic mice elicits a selective arginine deficiency and affects skin, muscle, and lymphoid development. Am J Clin Nutr 76(1):128-40. [PubMed: 12081826]  [MGI Ref ID J:80556]

de Jonge WJ; Kwikkers KL; te Velde AA; van Deventer SJ; Nolte MA; Mebius RE; Ruijter JM; Lamers MC; Lamers WH. 2002. Arginine deficiency affects early B cell maturation and lymphoid organ development in transgenic mice. J Clin Invest 110(10):1539-48. [PubMed: 12438451]  [MGI Ref ID J:80204]

de Vasconcelos AP; Bouilleret V; Riban V; Wasterlain C; Nehlig A. 2005. Role of nitric oxide in cerebral blood flow changes during kainate seizures in mice: genetic and pharmacological approaches. Neurobiol Dis 18(2):270-81. [PubMed: 15686955]  [MGI Ref ID J:124446]

van't Hof RJ; Macphee J; Libouban H; Helfrich MH; Ralston SH. 2004. Regulation of bone mass and bone turnover by neuronal nitric oxide synthase. Endocrinology 145(11):5068-74. [PubMed: 15297441]  [MGI Ref ID J:105612]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX1

Colony Maintenance

Breeding & HusbandryThis strain is maintained by breeding homozygous siblings. Colonies should be supervised closely to monitor for aggression in males although The Jackson Laboratory colony has not observed this behavior. Coat Color expected from breeding:Black
Mating SystemHomozygote x Homozygote         (Female x Male)
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Weeks of AgePrice*GenderGenotypes Provided
3-5 weeks $73.60Female or MaleHomozygous for Nos1tm1Plh
6 weeks $77.50Female or MaleHomozygous for Nos1tm1Plh
7 weeks $81.40Female or MaleHomozygous for Nos1tm1Plh
8 weeks $85.30Female or MaleHomozygous for Nos1tm1Plh
9 weeks $89.20Female or MaleHomozygous for Nos1tm1Plh
10 weeks $93.10Female or MaleHomozygous for Nos1tm1Plh
Pairs /Price*Pair Genotype
$155.00Homozygous for Nos1tm1Plh x Homozygous for Nos1tm1Plh
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Pricing for International shipping destinations View USA Canada and Mexico pricing
Weeks of AgePrice*GenderGenotypes Provided
3-5 weeks $95.70Female or MaleHomozygous for Nos1tm1Plh
6 weeks $100.80Female or MaleHomozygous for Nos1tm1Plh
7 weeks $105.90Female or MaleHomozygous for Nos1tm1Plh
8 weeks $111.00Female or MaleHomozygous for Nos1tm1Plh
9 weeks $116.10Female or MaleHomozygous for Nos1tm1Plh
10 weeks $121.20Female or MaleHomozygous for Nos1tm1Plh
Pairs /Price*Pair Genotype
$201.50Homozygous for Nos1tm1Plh x Homozygous for Nos1tm1Plh
*Price(s) in US dollars ($)

Additional Supply Details

Supply Notes

Supply Details

Standard SupplyLevel 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes
  • Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available.
  • Strains that must be genotyped are not available until five to seven weeks of age.
  • This strain is included in the Induced Mutant Resource Colony collection.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. THE LABORATORY EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.


(3.2)