Description

Strain Information

Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N?+3   Donating Investigator Walter Koch,   Duke University Medical Center

Appearance
multiple coat colors
Related Genotype: segregating for a, A, Oca2^p, Tyr^c and Pde6b^rd1

Description
Mice carrying the CAMalpha1b transgene display stable myocardial specific hypertrophy with ensuing markers of hypertrophy being elevated. In addition, alpha 1 adrenergic receptor signaling through the Gq-phospholipase C-protein kinase C signaling pathway is enhanced.

Development
This strain was developed in the laboratory of Dr. Walter Koch at Duke University Medical Center. The transgene consists of a constitutively active form of the murine adrenergic receptor, alpha 1b, a SV40 introl/polyA sequence used for screening of mice, and the murine alpha-myosin heavy chain promoter.

Control Information

	Control
	Noncarrier
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Adra1b

004159

B6.129P2-Adra1btm1Cta/J

View Strains carrying other alleles of Adra1b     (1 strain)

Strains carrying other alleles of Myh6

002640	B6;SJL-Tg(Myh6-ADRBK1)27Wjk/J
002639	B6SJL-Tg(Myh6-ADRBK1)12Wjk/J
002638	B6SJL-Tg(WTbeta2)4Wjk/J
006768	D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ
002535	FVB/N-Tg(ANX6)2Agh/J

View Strains carrying other alleles of Myh6     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(CAMalpha1b)7Wjk/?

        Background Not Specified

• cardiovascular system phenotype
• *normal* cardiovascular system phenotype (MGI Ref ID J:82721)
  • systolic and diastolic blood pressure is normal in these mice
• abnormal cardiac muscle morphology (MGI Ref ID J:82721)
  • heart muscle contains elevated levels of diacylglycerol resulting from enhanced signaling by the alpha 1 adrenergic receptor through the Gq-phospholipase C-protein kinase C signaling pathway
• increased heart weight (MGI Ref ID J:82721)
  • the heart to body weight ratio is increased by 20% compared to non-transgenic controls
• right ventricle hypertrophy (MGI Ref ID J:82721)
  • myocytes located in the right ventricle have a 62% higher cross-section than found in non-transgenic controls
• muscle phenotype
• abnormal cardiac muscle morphology (MGI Ref ID J:82721)
  • heart muscle contains elevated levels of diacylglycerol resulting from enhanced signaling by the alpha 1 adrenergic receptor through the Gq-phospholipase C-protein kinase C signaling pathway

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Adra1b related

Cardiovascular Research
Heart Abnormalities
Hypotension
Vascular Defects

Internal/Organ Research
Heart Abnormalities

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(CAMalpha1b)7Wjk
Allele Name		transgene insertion 7, Walter Koch
Allele Type		Transgenic (random, expressed)
Common Name(s)		(CAM)alpha1B-AR; CAM7; CAMalpha1B-AR;
Mutation Made By		Walter Koch,   Duke University Medical Center
Expressed Gene		Adra1b, adrenergic receptor, alpha 1b, mouse, laboratory
Promoter		Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, mouse, laboratory
Molecular Note		Sequence encoding hamster adrenergic receptor alpha 1b (ADRA1B), containing 3 missense mutations (R288K, K290H, A293L) to make it constitutively active, was adjoined to the mouse myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Cardiac-specific expression of the mutant ADRA1B was indicated by radio ligand binding assay to be approximately 3-fold higher than that of the endogenous Adra1b. In addition to line 7 (CAM7), lines 21 (CAM21) and 56 (CAM56) were also generated. They also overexpress by approximately 3-fold. [MGI Ref ID J:82721]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(CAMalpha1b)7Wjk, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Milano CA; Dolber PC; Rockman HA; Bond RA; Venable ME; Allen LF; Lefkowitz RJ. 1994. Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy. Proc Natl Acad Sci U S A 91(21):10109-13. [PubMed: 7937846]  [MGI Ref ID J:82721]

Additional References

Tg(CAMalpha1b)7Wjk related

Gao XM; Wang BH; Woodcock E; Du XJ. 2000. Expression of active alpha(1B)-adrenergic receptors in the heart does not alleviate ischemic reperfusion injury. J Mol Cell Cardiol 32(9):1679-86. [PubMed: 10966830]  [MGI Ref ID J:127816]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Noncarrier
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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