Strain Name:

B6SJL-Tg(WTbeta2)4Wjk/J

Stock Number:

002638

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Availability:

Cryopreserved - Ready for recovery

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Walter Koch,   Duke University Medical Center

Appearance
multiple coat colors
Related Genotype: segregating for a, A, Oca2p, Tyrc, and Pde6brd1

Description
These transgenic mice exhibit increased basal myocardial adenylyl cyclase activity, enhanced atrial contractility, and increased left ventricular function in vivo; these parameters at baseline in the transgenic animals were equal to those observed in control animals maximally stimulated with isoproterenol. Mice carrying this transgene display a marked overexpression of the human beta-2 adrenergic receptor (~200 fold increase).

Development
This strain was developed in the laboratory of Dr. Walter Koch at Duke University Medical Center. The transgene consists of the human beta-2 adrenergic receptor, a SV40 introl/polyA sequence used for screening of mice, and the murine alpha-myosin heavy chain promoter. Transgenic mice were created with cardiac-specific overexpression of the Beta2 adrenergic receptor.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myh6
012389   B6.Cg-Tg(Myh6-Ppara)404-3Dpk/J
012382   B6.Cg-Tg(Myh6-Ppara)404-4Dpk/J
005657   B6.FVB(129)-Tg(Myh6-cre/Esr1*)1Jmk/J
011038   B6.FVB-Tg(Myh6-cre)2182Mds/J
013781   B6;FVB-Tg(Myh6/NFAT-luc)1Jmol/J
002640   B6;SJL-Tg(Myh6-ADRBK1)27Wjk/J
012383   B6CBA-Tg(Myh6-Ppard)HEDpk/J
018972   B6N.FVB(B6)-Tg(Myh6-cre)2182Mds/J
002639   B6SJL-Tg(Myh6-ADRBK1)12Wjk/J
006768   D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ
010587   FVB-Tg(Myh6-MEF2A)1Jmol/J
010581   FVB-Tg(Myh6-Map2k1*)1Jmol/J
010582   FVB-Tg(Myh6-Map2k3*)1Jmol/J
010584   FVB-Tg(Myh6-Map2k7)1Jmol/J
010585   FVB-Tg(Myh6-Mapk1)1Jmol/J
010583   FVB-Tg(Myh6-Mapk14*)1Jmol/J
010586   FVB-Tg(Myh6-Mef2c)2Jmol/J
009438   FVB-Tg(Myh6-SOD2,Tyr)3Pne/J
016570   FVB-Tg(Myh6-TRPC3*)6.6Jmol/J
017543   FVB-Tg(Myh6-TRPC6*)1Jmol/J
011037   FVB-Tg(Myh6-cre)2182Mds/J
010588   FVB-Tg(Myh6/NFAT-luc)1Jmol/J
017542   FVB-Tg(Myh6/tetO-ATP2B4)1Jmol/J
016571   FVB-Tg(Myh6/tetO-Gata6)2Jmol/J
014155   FVB-Tg(Myh6/tetO-Itpr1)22.3Jmol/J
014153   FVB-Tg(Myh6/tetO-Itpr2)3.11Jmol/J
012684   FVB-Tg(Myh6/tetO-POSTN)22.1Jmol/J
010580   FVB-Tg(Myh6/tetO-PRKCA*)1Jmk/J
022979   FVB-Tg(tetO-Thbs4)17.7Jmol/J
002535   FVB/N-Tg(ANX6)2Agh/J
012459   FVB/N-Tg(Myh6*/tetO-Capn1)L2Gwd/J
008716   FVB/N-Tg(Myh6-AIP/PLN*)46Jded/J
012461   FVB/N-Tg(Myh6-Cast)1Gwd/J
012460   FVB/N-Tg(Myh6-Gnaq)40Gwd/J
012477   STOCK Tg(Myh6*/tetO-GCaMP2)1Mik/J
009075   STOCK Tg(Myh6-Ppp3ca)37Eno/J
010579   STOCK Tg(Myh6-Prkca)1Jmk/J
009074   STOCK Tg(Myh6-cre)1Jmk/J
005650   STOCK Tg(Myh6-cre/Esr1*)1Jmk/J
016572   STOCK Tg(Myh6/tetO-Gata4)1Jmol/J
View Strains carrying other alleles of Myh6     (40 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Asthma, Susceptibility to   (ADRB2)
Beta-2-Adrenergic Receptor; ADRB2   (ADRB2)
Obesity   (ADRB2)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(WTbeta2)4Wjk/0

        B6.Cg-Tg(WTbeta2)4Wjk
  • mortality/aging
  • premature death
    • mean survival time is 307 days compared to over 600 for control mice and most mice die by 1 year of age   (MGI Ref ID J:125581)
  • behavior/neurological phenotype
  • fatigue
    • fatigue occurs in mice when approaching 1 year of age   (MGI Ref ID J:125581)
  • respiratory system phenotype
  • respiratory distress
    • occurs in mice when approaching 1 year of age   (MGI Ref ID J:125581)
  • homeostasis/metabolism phenotype
  • cyanosis
    • occurs in mucosal membranes as mice approach 1 year of age   (MGI Ref ID J:125581)

Tg(WTbeta2)4Wjk/0

        involves: C57BL/6
  • mortality/aging
  • premature death
    • mice show increased mortality by one year compared to controls   (MGI Ref ID J:134706)
  • cardiovascular system phenotype
  • abnormal cardiac muscle contractility
    • mice have increased fractional shortening at 4 months of age but not 8 months of age   (MGI Ref ID J:134706)
    • by 12 months of age, fractional shortening has become significantly impaired   (MGI Ref ID J:134706)
  • cardiac fibrosis
    • hearts of 8 month old mice show area of significant fibrosis   (MGI Ref ID J:134706)
  • homeostasis/metabolism phenotype
  • increased aerobic running capacity
    • mice have increased exercise tolerance on a treadmill compared to controls at 6 months of age   (MGI Ref ID J:134706)
  • muscle phenotype
  • abnormal cardiac muscle contractility
    • mice have increased fractional shortening at 4 months of age but not 8 months of age   (MGI Ref ID J:134706)
    • by 12 months of age, fractional shortening has become significantly impaired   (MGI Ref ID J:134706)

Tg(WTbeta2)4Wjk/?

        Background Not Specified
  • cardiovascular system phenotype
  • abnormal cardiac muscle relaxation
    • left ventricle relaxation is enhanced compared to controls with a mean drop in pressure of 5328 mm Hg versus 3313 mm Hg   (MGI Ref ID J:17862)
  • increased cardiac muscle contractility
    • the isometric tension of isolated left atria is three times that of controls while the isometric tension of the right atria is about 75% higher than in wild-type controls   (MGI Ref ID J:17862)
    • these parameters at baseline are equal to those observed in control mice maximally stimulated with isoproterenol   (MGI Ref ID J:17862)
    • increased ventricle muscle contractility
      • the baseline left ventricle dP/dT-max is 70% higher than in controls   (MGI Ref ID J:17862)
      • administration of isoproterenol fails to increase the dP/dTmax as it does in wild-type controls   (MGI Ref ID J:17862)
      • this parameter at baseline is equal to those observed in control mice maximally stimulated with isoproterenol   (MGI Ref ID J:17862)
  • increased heart rate
    • the mean heart rate is about 425 bpm compared to 340 bpm in control mice   (MGI Ref ID J:17862)
    • there is about a 50% increase in the heart rate of isolated right atria   (MGI Ref ID J:17862)
  • muscle phenotype
  • abnormal cardiac muscle relaxation
    • left ventricle relaxation is enhanced compared to controls with a mean drop in pressure of 5328 mm Hg versus 3313 mm Hg   (MGI Ref ID J:17862)
  • increased cardiac muscle contractility
    • the isometric tension of isolated left atria is three times that of controls while the isometric tension of the right atria is about 75% higher than in wild-type controls   (MGI Ref ID J:17862)
    • these parameters at baseline are equal to those observed in control mice maximally stimulated with isoproterenol   (MGI Ref ID J:17862)
    • increased ventricle muscle contractility
      • the baseline left ventricle dP/dT-max is 70% higher than in controls   (MGI Ref ID J:17862)
      • administration of isoproterenol fails to increase the dP/dTmax as it does in wild-type controls   (MGI Ref ID J:17862)
      • this parameter at baseline is equal to those observed in control mice maximally stimulated with isoproterenol   (MGI Ref ID J:17862)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

ADRB2 related

Cardiovascular Research
Heart Abnormalities

Internal/Organ Research
Heart Abnormalities

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(WTbeta2)4Wjk
Allele Name transgene insertion 4, Walter Koch
Allele Type Transgenic (Inserted expressed sequence)
Common Name(s) TG4; Tg(Myh6-ADRB2)4Wjk;
Mutation Made ByDr. Walter Koch,   Duke University Medical Center
Expressed Gene ADRB2, adrenoceptor beta 2, surface, human
Promoter Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine
Molecular Note The transgene consists of the human beta-2 adrenergic receptor (ADRB2), an SV40 intron/polyA sequence, and the murine myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter which directs cardiac-specific overexpression of ADRB2. Transgene expression is detected in cardiac myocytes at levels 200 times greater than in human cardiac tissue. [MGI Ref ID J:17862]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(WTbeta2)4Wjk, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Milano CA; Allen LF; Rockman HA; Dolber PC; McMinn TR; Chien KR; Johnson TD; Bond RA; Lefkowitz RJ. 1994. Enhanced myocardial function in transgenic mice overexpressing the beta 2-adrenergic receptor [see comments] Science 264(5158):582-6. [PubMed: 8160017]  [MGI Ref ID J:17862]

Additional References

An RH; Heath BM; Higgins JP; Koch WJ; Lefkowitz RJ; Kass RS. 1999. beta(2)-adrenergic receptor overexpression in the developing mouse heart: evidence for targeted modulation of ion channels. J Physiol 516 (Pt 1):19-30. [PubMed: 10066919]  [MGI Ref ID J:55224]

Dorn GW 2nd; Tepe NM; Lorenz JN; Koch WJ; Liggett SB. 1999. Low- and high-level transgenic expression of beta2-adrenergic receptors differentially affect cardiac hypertrophy and function in Galphaq- overexpressing mice. Proc Natl Acad Sci U S A 96(11):6400-5. [PubMed: 10339599]  [MGI Ref ID J:55199]

Tg(WTbeta2)4Wjk related

An RH; Heath BM; Higgins JP; Koch WJ; Lefkowitz RJ; Kass RS. 1999. beta(2)-adrenergic receptor overexpression in the developing mouse heart: evidence for targeted modulation of ion channels. J Physiol 516 (Pt 1):19-30. [PubMed: 10066919]  [MGI Ref ID J:55224]

Cross HR; Murphy E; Koch WJ; Steenbergen C. 2002. Male and female mice overexpressing the beta(2)-adrenergic receptor exhibit differences in ischemia/reperfusion injury: role of nitric oxide. Cardiovasc Res 53(3):662-71. [PubMed: 11861037]  [MGI Ref ID J:162751]

Dilly KW; Kurokawa J; Terrenoire C; Reiken S; Lederer WJ; Marks AR; Kass RS. 2004. Overexpression of beta2-adrenergic receptors cAMP-dependent protein kinase phosphorylates and modulates slow delayed rectifier potassium channels expressed in murine heart: evidence for receptor/channel co-localization. J Biol Chem 279(39):40778-87. [PubMed: 15272004]  [MGI Ref ID J:93340]

Dorn GW 2nd; Tepe NM; Lorenz JN; Koch WJ; Liggett SB. 1999. Low- and high-level transgenic expression of beta2-adrenergic receptors differentially affect cardiac hypertrophy and function in Galphaq- overexpressing mice. Proc Natl Acad Sci U S A 96(11):6400-5. [PubMed: 10339599]  [MGI Ref ID J:55199]

Du XJ; Autelitano DJ; Dilley RJ; Wang B; Dart AM; Woodcock EA. 2000. beta(2)-adrenergic receptor overexpression exacerbates development of heart failure after aortic stenosis. Circulation 101(1):71-7. [PubMed: 10618307]  [MGI Ref ID J:60080]

Du XJ; Gao XM; Wang B; Jennings GL; Woodcock EA; Dart AM. 2000. Age-dependent cardiomyopathy and heart failure phenotype in mice overexpressing beta(2)-adrenergic receptors in the heart. Cardiovasc Res 48(3):448-54. [PubMed: 11090840]  [MGI Ref ID J:128583]

Foerster K; Groner F; Matthes J; Koch WJ; Birnbaumer L; Herzig S. 2003. Cardioprotection specific for the G protein Gi2 in chronic adrenergic signaling through beta 2-adrenoceptors. Proc Natl Acad Sci U S A 100(24):14475-80. [PubMed: 14612574]  [MGI Ref ID J:125581]

Freeman K; Lerman I; Kranias EG; Bohlmeyer T; Bristow MR; Lefkowitz RJ; Iaccarino G; Koch WJ; Leinwand LA. 2001. Alterations in cardiac adrenergic signaling and calcium cycling differentially affect the progression of cardiomyopathy. J Clin Invest 107(8):967-74. [PubMed: 11306600]  [MGI Ref ID J:134706]

Gaussin V; Tomlinson JE; Depre C; Engelhardt S; Antos CL; Takagi G; Hein L; Topper JN; Liggett SB; Olson EN; Lohse MJ; Vatner SF; Vatner DE. 2003. Common genomic response in different mouse models of beta-adrenergic-induced cardiomyopathy. Circulation 108(23):2926-33. [PubMed: 14623810]  [MGI Ref ID J:102945]

Grandy SA; Denovan-Wright EM; Ferrier GR; Howlett SE. 2004. Overexpression of human beta2-adrenergic receptors increases gain of excitation-contraction coupling in mouse ventricular myocytes. Am J Physiol Heart Circ Physiol 287(3):H1029-38. [PubMed: 15155261]  [MGI Ref ID J:95592]

Hasseldine AR; Harper EA; Black JW. 2003. Cardiac-specific overexpression of human beta2 adrenoceptors in mice exposes coupling to both Gs and Gi proteins. Br J Pharmacol 138(7):1358-66. [PubMed: 12711637]  [MGI Ref ID J:133731]

Heubach JF; Trebess I; Wettwer E; Himmel HM; Michel MC; Kaumann AJ; Koch WJ; Harding SE; Ravens U. 1999. L-type calcium current and contractility in ventricular myocytes from mice overexpressing the cardiac beta 2-adrenoceptor. Cardiovasc Res 42(1):173-82. [PubMed: 10435008]  [MGI Ref ID J:130595]

Peter PS; Brady JE; Yan L; Chen W; Engelhardt S; Wang Y; Sadoshima J; Vatner SF; Vatner DE. 2007. Inhibition of p38 alpha MAPK rescues cardiomyopathy induced by overexpressed beta 2-adrenergic receptor, but not beta 1-adrenergic receptor. J Clin Invest 117(5):1335-43. [PubMed: 17446930]  [MGI Ref ID J:122039]

Rockman HA; Chien KR; Choi DJ; Iaccarino G; Hunter JJ; Ross J Jr; Lefkowitz RJ; Koch WJ. 1998. Expression of a beta-adrenergic receptor kinase 1 inhibitor prevents the development of myocardial failure in gene-targeted mice. Proc Natl Acad Sci U S A 95(12):7000-5. [PubMed: 9618528]  [MGI Ref ID J:48062]

Sheridan DJ; Autelitano DJ; Wang B; Percy E; Woodcock EA; Du XJ. 2000. Beta(2)-adrenergic receptor overexpression driven by alpha-MHC promoter is downregulated in hypertrophied and failing myocardium. Cardiovasc Res 47(1):133-41. [PubMed: 10869539]  [MGI Ref ID J:162755]

Thireau J; Aimond F; Poisson D; Zhang B; Bruneval P; Eder V; Richard S; Babuty D. 2010. New insights into sexual dimorphism during progression of heart failure and rhythm disorders. Endocrinology 151(4):1837-45. [PubMed: 20176721]  [MGI Ref ID J:168521]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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