Description

Strain Information

Former Names B6SJL-Tg(BARK12)12Wjk/J    (Changed: 04-MAY-07 ) B6SJL-TgN(BARK12)12Wjk    (Changed: 15-DEC-04 ) B6SJL-TgN(BARK12)12Wjk/J    (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N8   Donating Investigator Walter Koch,   Duke University Medical Center

Description
Mice carrying this transgene show a 3 to 5 fold overexpression of bovine beta-adrenergic receptor kinase-1. Myocardial beta-adrenergic receptors are hyperphosphorylated and desensitized in hemizygotes resulting in attenuated cardiac function in response to beta-agonists.

Control Information

	Control
	Noncarrier
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myh6

002640	B6;SJL-Tg(Myh6-ADRBK1)27Wjk/J
002637	B6SJL-Tg(CAMalpha1b)7Wjk/J
002638	B6SJL-Tg(WTbeta2)4Wjk/J
006768	D2.Cg-Tg(Myh6-Zfpm2)1Sho/EiJ
002535	FVB/N-Tg(ANX6)2Agh/J

View Strains carrying other alleles of Myh6     (5 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(Myh6-ADRBK1)12Wjk/?

        involves: C57BL/6 * SJL

• cardiovascular system phenotype
• abnormal heart rate (MGI Ref ID J:37034)
  • heart rate failed to increase in response to the beta-agonist isoproterenol
  • rises in pressure induced by the beta-agonist isoproterenol are depressed in these mice by about 20% at the highest dose
• increased left ventricle diastolic pressure (MGI Ref ID J:37034)
  • there is a blunted response to rises in pressure induced by the beta-agonist isoproterenol
  • rises in pressure induced by the beta-agonist isoproterenol are depressed in these mice by about 20% at the highest dose

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cardiovascular Research
Heart Abnormalities

Internal/Organ Research
Heart Abnormalities

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(Myh6-ADRBK1)12Wjk
Allele Name		transgene insertion 12, Walter Koch
Allele Type		Transgenic (random, expressed)
Common Name(s)		BetaARK1 transgene; TGBetaK12; Tg(BARK12)12Wjk;
Mutation Made By		Walter Koch,   Duke University Medical Center
Strain of Origin		(C57BL/6 x SJL)F1
Expressed Gene		ADRBK1, ADRBK1, pig, domestic
Promoter		Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, mouse, laboratory
Molecular Note		The entire bovine adrenergic receptor kinase, beta 1 (ADRBK1) coding region was ligated to a 5.5 kb fragment of the murine adult myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. Overexpression of the transgenic protein was detected by Western blot analysis of heart extracts. Biochemical assays showed that kinase activity was approximately 3 times greater in heart extracts from transgenic mice than from wild-type mice. The Myh6 promoter drives expression in myocardial tissue. [MGI Ref ID J:37034]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Myh6-ADRBK1)12Wjk, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Koch WJ; Rockman HA; Samama P; Hamilton RA; Bond RA; Milano CA; Lefkowitz RJ. 1995. Cardiac function in mice overexpressing the beta-adrenergic receptor kinase or a beta ARK inhibitor. Science 268(5215):1350-3. [PubMed: 7761854]  [MGI Ref ID J:37034]

Additional References

Tg(Myh6-ADRBK1)12Wjk related

Akhter SA; Eckhart AD; Rockman HA; Shotwell K; Lefkowitz RJ; Koch WJ. 1999. In vivo inhibition of elevated myocardial beta-adrenergic receptor kinase activity in hybrid transgenic mice restores normal beta-adrenergic signaling and function. Circulation 100(6):648-53. [PubMed: 10441103]  [MGI Ref ID J:127858]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Noncarrier
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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