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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names B6;SJL-Tg(minBARKct)27Wjk/J (Changed: 04-MAY-07 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F8p
Generation DefinitionsDonating Investigator Walter Koch, Duke University Medical Center Description
Mice carrying this transgene overexpress a truncated form of the bovine beta-adrenergic receptor kinase-1 containing only the last 194 amino acids of the ADRBK1 protein. The truncated protein inhibits endogenous beta-adrenergic receptor kinase-1 by blocking beta-adrenergic receptor desensitization. This myocardial inhibition increases cardiac function of homozygotes under basal conditions and leads to supersensitization to beta-agonists. This strain may serve as a model for increased left ventricular performance, a novel method of positive intropy, and enhanced beta-adrenergic signalling.
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| 000686 SJL/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Myh6
View Strains carrying other alleles of Myh6 (38 strains)
Phenotype
Phenotype Information
assigned by genotype
Tg(Myh6-ADRBK1)27Wjk/0
involves: C57BL/6 * SJL
- cardiovascular system phenotype
- increased cardiac muscle contractility
- mice have increased fractional shortening from 4 to at least 12 months of age (MGI Ref ID J:134706)
- increased left ventricle diastolic pressure
- both the left ventricular dP/dTmin and dP/dTmax are significantly increased at baseline (MGI Ref ID J:37034)
- increases in pressure are enhanced with administration of the beta-agonist isoproterenol (MGI Ref ID J:37034)
- both the left ventricular dP/dTmin and dP/dTmax are significantly increased about 25% at baseline (MGI Ref ID J:127858)
- increases in pressure are enhanced with administration of the beta-agonist isoproterenol (MGI Ref ID J:127858)
- increased left ventricle systolic pressure
- muscle phenotype
- increased cardiac muscle contractility
- mice have increased fractional shortening from 4 to at least 12 months of age (MGI Ref ID J:134706)
This mouse can be used to support research in many areas including:
Cardiovascular Research
Heart Abnormalities
Internal/Organ Research
Heart Abnormalities
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Myh6-ADRBK1)27Wjk | ||
|---|---|---|---|
| Allele Name | transgene insertion 27, Walter Koch | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | BetaARKct; TG betaARKct; TGMini27; Tg(minBARKct)27Wjk; | ||
| Mutation Made By | Walter Koch, Duke University Medical Center | ||
| Strain of Origin | (C57BL/6 x SJL)F1 | ||
| Expressed Gene | ADRBK1, adrenergic, beta, receptor kinase 1, human | ||
| Promoter | Myh6, myosin, heavy polypeptide 6, cardiac muscle, alpha, murine, murine | ||
| Molecular Note | Sequence encoding the carboxyl terminus of the bovine adrenergic receptor kinase, beta 1 (ADRBK1) (residues 495 through 689) was ligated to a 5.5 kb fragment of the murine adult myosin, heavy polypeptide 6, cardiac muscle, alpha (Myh6) promoter. The resultant 194 residue carboxyl terminal peptide was detected by Western blot analysis and inhibits endogenous beta-adrenergic receptor kinase-1 by blocking beta-adrenergic receptor desensitization. The Myh6 promoter drives expression in myocardial tissue. [MGI Ref ID J:37034] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Myh6-ADRBK1)27Wjk, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Koch WJ; Rockman HA; Samama P; Hamilton RA; Bond RA; Milano CA; Lefkowitz RJ. 1995. Cardiac function in mice overexpressing the beta-adrenergic receptor kinase or a beta ARK inhibitor. Science 268(5215):1350-3. [PubMed: 7761854] [MGI Ref ID J:37034]
Additional References
Tg(Myh6-ADRBK1)27Wjk relatedAkhter SA; Eckhart AD; Rockman HA; Shotwell K; Lefkowitz RJ; Koch WJ. 1999. In vivo inhibition of elevated myocardial beta-adrenergic receptor kinase activity in hybrid transgenic mice restores normal beta-adrenergic signaling and function. Circulation 100(6):648-53. [PubMed: 10441103] [MGI Ref ID J:127858]
Brinks H; Boucher M; Gao E; Chuprun JK; Pesant S; Raake PW; Huang ZM; Wang X; Qiu G; Gumpert A; Harris DM; Eckhart AD; Most P; Koch WJ. 2010. Level of G protein-coupled receptor kinase-2 determines myocardial ischemia/reperfusion injury via pro- and anti-apoptotic mechanisms. Circ Res 107(9):1140-9. [PubMed: 20814022] [MGI Ref ID J:178191]
Dorn GW 2nd; Tepe NM; Lorenz JN; Koch WJ; Liggett SB. 1999. Low- and high-level transgenic expression of beta2-adrenergic receptors differentially affect cardiac hypertrophy and function in Galphaq- overexpressing mice. Proc Natl Acad Sci U S A 96(11):6400-5. [PubMed: 10339599] [MGI Ref ID J:55199]
Eckhart AD; Fentzke RC; Lepore J; Lang R; Lin H; Lefkowitz RJ; Koch WJ; Leiden JM. 2002. Inhibition of betaARK1 restores impaired biochemical beta-adrenergic receptor responsiveness but does not rescue CREB(A133) induced cardiomyopathy. J Mol Cell Cardiol 34(6):669-77. [PubMed: 12054854] [MGI Ref ID J:127503]
Esposito G; Prasad SV; Rapacciuolo A; Mao L; Koch WJ; Rockman HA. 2001. Cardiac overexpression of a G(q) inhibitor blocks induction of extracellular signal-regulated kinase and c-Jun NH(2)-terminal kinase activity in in vivo pressure overload. Circulation 103(10):1453-8. [PubMed: 11245652] [MGI Ref ID J:135011]
Freeman K; Lerman I; Kranias EG; Bohlmeyer T; Bristow MR; Lefkowitz RJ; Iaccarino G; Koch WJ; Leinwand LA. 2001. Alterations in cardiac adrenergic signaling and calcium cycling differentially affect the progression of cardiomyopathy. J Clin Invest 107(8):967-74. [PubMed: 11306600] [MGI Ref ID J:134706]
Rockman HA; Chien KR; Choi DJ; Iaccarino G; Hunter JJ; Ross J Jr; Lefkowitz RJ; Koch WJ. 1998. Expression of a beta-adrenergic receptor kinase 1 inhibitor prevents the development of myocardial failure in gene-targeted mice. Proc Natl Acad Sci U S A 95(12):7000-5. [PubMed: 9618528] [MGI Ref ID J:48062]
Rockman HA; Choi DJ; Akhter SA; Jaber M; Giros B; Lefkowitz RJ; Caron MG; Koch WJ. 1998. Control of myocardial contractile function by the level of beta-adrenergic receptor kinase 1 in gene-targeted mice. J Biol Chem 273(29):18180-4. [PubMed: 9660778] [MGI Ref ID J:48681]
Tachibana H; Naga Prasad SV; Lefkowitz RJ; Koch WJ; Rockman HA. 2005. Level of beta-adrenergic receptor kinase 1 inhibition determines degree of cardiac dysfunction after chronic pressure overload-induced heart failure. Circulation 111(5):591-7. [PubMed: 15668342] [MGI Ref ID J:108555]
Tong H; Rockman HA; Koch WJ; Steenbergen C; Murphy E. 2004. G protein-coupled receptor internalization signaling is required for cardioprotection in ischemic preconditioning. Circ Res 94(8):1133-41. [PubMed: 15031261] [MGI Ref ID J:98928]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.
Purchasing information
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $1980.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
|
|
Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $2574.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
- Cryorecovery - Standard.
We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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|
|
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
General Supply Notes
- This strain is included in the Induced Mutant Resource Colony collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| 000686 SJL/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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