Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F10pN1 (29-MAY-05)   Donating Investigator Albee Messing,   University of Wisconsin-Madison

Appearance
white-bellied agouti
Related Genotype: A^w/A^w

Description
Mice homozygous for the Gfap^tm1Me targeted mutation are viable and fertile. Homozygous mutant mice show subtle changes in astrocyte morphology, CNS physiology, and enhanced LTP of both population spike amplitude and EPSP slope compared to control mice. Homozygotes are also hypersensitive to cervical spinal cord injury. These mice may be useful in studies dealing with transplantation, injury, or astrocyte biology.

Development
This strain was developed in the laboratory of Dr. Albee Messing at the University of Wisconsin School of Veterinary Medicine.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Gfap

005698

B6.Cg-Tg(Gfap-Tk)7.1Mvs/J

View Strains carrying other alleles of Gfap     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Gfap^tm1Mes/Gfap^tm1Mes

        either: (involves: 129S7/SvEvBrd * 129/Sv) or (involves: 129S7/SvEvBrd * C57BL/6)

• nervous system phenotype
• abnormal astrocyte morphology (MGI Ref ID J:64290)
• abnormal long term potentiation (MGI Ref ID J:64290)
• homeostasis/metabolism phenotype
• increased susceptibility to injury (MGI Ref ID J:48767)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Gfap^tm1Mes related

Neurobiology Research
Astrocyte Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Gfaptm1Mes
Allele Name		targeted mutation 1, Albee Messing
Allele Type		Targeted (knock-out)
Mutation Made By		Albee Messing,   University of Wisconsin-Madison
Strain of Origin		129S7/SvEvBrd-Hprt1+
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name		Gfap, glial fibrillary acidic protein
Chromosome		11
Gene Common Name(s)		AI836096; FLJ45472; expressed sequence AI836096;
Molecular Note		A neomycin cassette replaced most of exon 1 and all of exons 2-4. Northern blot analysis revealed that there was no mRNA produced from this allele in whole brain RNA samples derived from homozygous mice. Immunohistochemical studies detected no GFAP protein present in sections of hippocampus in homozygous mice. [MGI Ref ID J:64290]

Genotyping

Genotyping Information

Genotyping Protocols

Gfap^tm1Mes, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

McCall MA; Gregg RG; Behringer RR; Brenner M; Delaney CL; Galbreath EJ; Zhang CL; Pearce RA; Chiu SY; Messing A. 1996. Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology. Proc Natl Acad Sci U S A 93(13):6361-6. [PubMed: 8692820]  [MGI Ref ID J:64290]

Additional References

Hanbury R; Ling ZD; Wuu J; Kordower JH. 2003. GFAP knockout mice have increased levels of GDNF that protect striatal neurons from metabolic and excitotoxic insults. J Comp Neurol 461(3):307-16. [PubMed: 12746870]  [MGI Ref ID J:83650]

Hughes EG; Maguire JL; McMinn MT; Scholz RE; Sutherland ML. 2004. Loss of glial fibrillary acidic protein results in decreased glutamate transport and inhibition of PKA-induced EAAT2 cell surface trafficking. Brain Res Mol Brain Res 124(2):114-23. [PubMed: 15135219]  [MGI Ref ID J:91242]

Nawashiro H; Messing A; Azzam N; Brenner M. 1998. Mice lacking GFAP are hypersensitive to traumatic cerebrospinal injury. Neuroreport 9(8):1691-6. [PubMed: 9665584]  [MGI Ref ID J:48767]

Gfap^tm1Mes related

Hagemann TL; Connor JX; Messing A. 2006. Alexander disease-associated glial fibrillary acidic protein mutations in mice induce Rosenthal fiber formation and a white matter stress response. J Neurosci 26(43):11162-73. [PubMed: 17065456]  [MGI Ref ID J:114689]

Hanbury R; Ling ZD; Wuu J; Kordower JH. 2003. GFAP knockout mice have increased levels of GDNF that protect striatal neurons from metabolic and excitotoxic insults. J Comp Neurol 461(3):307-16. [PubMed: 12746870]  [MGI Ref ID J:83650]

Hughes EG; Maguire JL; McMinn MT; Scholz RE; Sutherland ML. 2004. Loss of glial fibrillary acidic protein results in decreased glutamate transport and inhibition of PKA-induced EAAT2 cell surface trafficking. Brain Res Mol Brain Res 124(2):114-23. [PubMed: 15135219]  [MGI Ref ID J:91242]

Nawashiro H; Messing A; Azzam N; Brenner M. 1998. Mice lacking GFAP are hypersensitive to traumatic cerebrospinal injury. Neuroreport 9(8):1691-6. [PubMed: 9665584]  [MGI Ref ID J:48767]

Triolo D; Dina G; Lorenzetti I; Malaguti M; Morana P; Del Carro U; Comi G; Messing A; Quattrini A; Previtali SC. 2006. Loss of glial fibrillary acidic protein (GFAP) impairs Schwann cell proliferation and delays nerve regeneration after damage. J Cell Sci 119(Pt 19):3981-93. [PubMed: 16988027]  [MGI Ref ID J:113190]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

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