Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+17p (23-MAY-04)   Donating Investigator Thomas O'Brien,   The Lankenau Medical Research Center

Appearance
black, hairless
Related Genotype: a/aTgN(K6ODCtr)55Tgo/-

Description
Mice carrying the (K6ODCtr)transgene show skin abnormalities including: alopecia, enhanced nail growth, follicular cysts in the dermis, excessive skin wrinkling and folding, oily skin, acne, and spontaneous squamous skin tumors. This strain may serve as a model for acne or for enhanced susceptibility to skin cancer. Mice homozygous for this transgene are not viable.

Development
The transgene consists of a mutant murine Odc gene with a premature stop codon at position 427, and the minimal promoter from the bovine keratin 6 gene homolog. This strain was developed in the laboratory of Dr. Thomas O'Brien at The Lankenau Medical Research Center.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

Atrichia with Papular Lesions; APL -

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(K6ODCtr)55Tgo/0

        C57BL/6-Tg(K6ODCtr)55Tgo/J

• skin/coat/nails phenotype
• abnormal hair follicle morphology (MGI Ref ID J:62356)
  • mice exhibit a dilatation of the hair follicle infundibulum and disintegration of the lower portion of the hair follicle
• abnormal nail morphology (MGI Ref ID J:62356)
  • mice exhibit excessive nail growth
• alopecia (MGI Ref ID J:62356)
  • by 1.5 months of age, mice exhibit generalized hair loss
  • cysts replace the lower portion of the hair follicle
• dermal cysts (MGI Ref ID J:62356)
  • cysts replace the lower portion of the hair follicle
  • some cysts have thick walls of stratified squamous epithelium filled with concentrically oriented cornified squames
  • in older mice, dermal cysts replace the subcutaneous fat layer
  • some cysts rupture and form foreign body granulomas
• enlarged sebaceous gland (MGI Ref ID J:62356)
  • sebaceous glands are moderately hyperplastic
• skin lesions (MGI Ref ID J:62356)
  • mice exhibit comedo-like structures associated with sebaceous glands
• wrinkled skin (MGI Ref ID J:62356)
  • as mice age their skin exhibits excessive growth resulting in wrinkling and folding
• touch/vibrissae phenotype
• absent vibrissae (MGI Ref ID J:62356)
  • by 1.5 months of age
• adipose tissue phenotype
• decreased subcutaneous adipose tissue amount (MGI Ref ID J:62356)
  • in older mice, dermal cysts replace the subcutaneous fat layer
• growth/size phenotype
• decreased body weight (MGI Ref ID J:62356)
  • at birth
• endocrine/exocrine gland phenotype
• enlarged sebaceous gland (MGI Ref ID J:62356)
  • sebaceous glands are moderately hyperplastic

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(K6ODCtr)55Tgo/0

        involves: C3H * C57BL/6

• skin/coat/nails phenotype
• abnormal nail morphology (MGI Ref ID J:29098)
  • mice exhibit excessive nail growth
• absent hair follicles (MGI Ref ID J:29098)
  • after 2 months of age
• alopecia (MGI Ref ID J:29098)
  • despite a normal first hair cycle, mice exhibit hair loss at 2 to 3 weeks of age
  • mice exhibit complete hair loss by 2 months of age
  • hair loss is a dominant phenotype
• dermal cysts (MGI Ref ID J:29098)
  • mice exhibit follicular cysts in the dermis
  • in mice older than 3 months, cysts are very large and replace the subcutaneous fat layer
• enlarged sebaceous gland (MGI Ref ID J:29098)
  • sebaceous glands near dermal cysts are moderately hyperplastic
• oily skin (MGI Ref ID J:29098)
  • develops with age
• skin lesions (MGI Ref ID J:29098)
  • mice develop keratoacanthom-like lesions
• skin tumor (MGI Ref ID J:29098)
  • mice develop skin tumors (squamous neoplasms) at a higher rate than wild-type mice
  • however, squamous cell carcinomas are not observed
• skin papilloma (MGI Ref ID J:29098)
  • mice develop keratoacanthom-like lesions and well-differentiated papillomatous lesions
  • skin tumors are found on the tail, face and ears
• wrinkled skin (MGI Ref ID J:29098)
  • develops with age
• adipose tissue phenotype
• decreased subcutaneous adipose tissue amount (MGI Ref ID J:29098)
  • after three months of age, dermal cysts replace the subcutaneous fat layer
• touch/vibrissae phenotype
• absent vibrissae (MGI Ref ID J:29098)
  • by 2 months of age
• growth/size phenotype
• decreased body weight (MGI Ref ID J:29098)
  • at birth
• cellular phenotype
• increased apoptosis (MGI Ref ID J:133302)
  • in culture, primary keratinocytes undergo premature cell death via apoptosis compared to wild-type cells
  • increased cell density prolongs primary keratinocyte cell life in culture but does not prevent premature death
  • however, treatment with DFMO blocks cell death in cultured primary keratinocytes
• tumorigenesis
• skin tumor (MGI Ref ID J:29098)
  • mice develop skin tumors (squamous neoplasms) at a higher rate than wild-type mice
  • however, squamous cell carcinomas are not observed
• skin papilloma (MGI Ref ID J:29098)
  • mice develop keratoacanthom-like lesions and well-differentiated papillomatous lesions
  • skin tumors are found on the tail, face and ears
• endocrine/exocrine gland phenotype
• enlarged sebaceous gland (MGI Ref ID J:29098)
  • sebaceous glands near dermal cysts are moderately hyperplastic

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Odc1 related

Cancer Research
Increased Tumor Incidence (Skin Cancers)
Toxicology

Dermatology Research
Skin and Hair Texture Defects

Metabolism Research

Research Tools
Cancer Research
Dermatology Research
Toxicology Research

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(K6ODCtr)55Tgo
Allele Name		transgene insertion 55, Thomas O'Brien
Allele Type		Transgenic (random, expressed)
Common Name(s)		K6/ODC;
Mutation Made By		Thomas O'Brien,   The Lankenau Medical Research Center
Strain of Origin		(C57BL/6 x C3H)F2
Expressed Gene		Odc1, ornithine decarboxylase, structural 1, mouse, laboratory
Promoter		KRT6, keratin 6A, cattle
Molecular Note		The transgene consists of a mutant murine Odc1 gene with a premature stop codon at position 427, and the minimal promoter from the bovine keratin 6 gene homolog.

Genotyping

Genotyping Information

Genotyping Protocols

Tg(K6ODCtr)55Tgo, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Megosh L; Gilmour SK; Rosson D; Soler AP; Blessing M; Sawicki JA; O'Brien TG. 1995. Increased frequency of spontaneous skin tumors in transgenic mice which overexpress ornithine decarboxylase. Cancer Res 55(19):4205-9. [PubMed: 7671221]  [MGI Ref ID J:29098]

Additional References

Morikawa T; Wanibuchi H; Morimura K; Ogawa M; Fukushima S. 2000. Promotion of skin carcinogenesis by dimethylarsinic acid in keratin (K6)/ODC transgenic mice. Jpn J Cancer Res 91(6):579-81. [PubMed: 10874208]  [MGI Ref ID J:62952]

Tg(K6ODCtr)55Tgo related

Chen Y; Megosh LC; Gilmour SK; Sawicki JA; O'Brien TG. 2000. K6/ODC transgenic mice as a sensitive model for carcinogen identification. Toxicol Lett 116(1-2):27-35. [PubMed: 10906419]  [MGI Ref ID J:63568]

George K; Iacobucci A; Uitto J; O'brien TG. 2005. Identification of an X-linked locus modifying mouse skin tumor susceptibility. Mol Carcinog 44(3):212-8. [PubMed: 16086382]  [MGI Ref ID J:101472]

Gilmour SK; Birchler M; Smith MK; Rayca K; Mostochuk J. 1999. Effect of elevated levels of ornithine decarboxylase on cell cycle progression in skin. Cell Growth Differ 10(11):739-48. [PubMed: 10593650]  [MGI Ref ID J:59581]

Hobbs CA; Wei G; Defeo K; Paul B; Hayes CS; Gilmour SK. 2006. Tip60 Protein Isoforms and Altered Function in Skin and Tumors that Overexpress Ornithine Decarboxylase. Cancer Res 66(16):8116-22. [PubMed: 16912189]  [MGI Ref ID J:113386]

Lan L; Trempus C; Gilmour SK. 2000. Inhibition of ornithine decarboxylase (ODC) decreases tumor vascularization and reverses spontaneous tumors in ODC/Ras transgenic mice Cancer Res 60(20):5696-703. [PubMed: 11059762]  [MGI Ref ID J:65347]

Megosh L; Halpern M; Farkash E; O'Brien TG. 1998. Analysis of ras gene mutational spectra in epidermal papillomas from K6/ODC transgenic mice. Mol Carcinog 22(3):145-9. [PubMed: 9688139]  [MGI Ref ID J:49929]

Miller TJ; Honchel R; Espandiari P; Knapton A; Zhang J; Sistare FD; Hanig JP. 2008. The utility of the K6/ODC transgenic mouse as an alternative short term dermal model for carcinogenicity testing of pharmaceuticals. Regul Toxicol Pharmacol 50(1):87-97. [PubMed: 18069108]  [MGI Ref ID J:133428]

Morikawa T; Wanibuchi H; Morimura K; Ogawa M; Fukushima S. 2000. Promotion of skin carcinogenesis by dimethylarsinic acid in keratin (K6)/ODC transgenic mice. Jpn J Cancer Res 91(6):579-81. [PubMed: 10874208]  [MGI Ref ID J:62952]

O'Brien TG; Megosh LC; Gilliard G; Soler AP. 1997. Ornithine decarboxylase overexpression is a sufficient condition for tumor promotion in mouse skin. Cancer Res 57(13):2630-7. [PubMed: 9205069]  [MGI Ref ID J:41488]

Panteleyev AA; Christiano AM; O'Brien TG; Sundberg JP. 2000. Ornithine decarboxylase transgenic mice as a model for human atrichia with papular lesions Exp Dermatol 9(2):146-51. [PubMed: 10772389]  [MGI Ref ID J:62356]

Peralta Soler A; Gilliard G; Megosh L; George K; O'Brien TG. 1998. Polyamines regulate expression of the neoplastic phenotype in mouse skin. Cancer Res 58(8):1654-9. [PubMed: 9563478]  [MGI Ref ID J:47024]

Smith MK; Trempus CS; Gilmour SK. 1998. Co-operation between follicular ornithine decarboxylase and v-Ha-ras induces spontaneous papillomas and malignant conversion in transgenic skin. Carcinogenesis 19(8):1409-15. [PubMed: 9744537]  [MGI Ref ID J:50136]

Tang X; Kim AL; Feith DJ; Pegg AE; Russo J; Zhang H; Aszterbaum M; Kopelovich L; Epstein EH Jr; Bickers DR; Athar M. 2004. Ornithine decarboxylase is a target for chemoprevention of basal and squamous cell carcinomas in Ptch1+/- mice. J Clin Invest 113(6):867-75. [PubMed: 15067319]  [MGI Ref ID J:89218]

Wei G; DeFeo K; Hayes CS; Woster PM; Mandik-Nayak L; Gilmour SK. 2008. Elevated ornithine decarboxylase levels activate ataxia telangiectasia mutated-DNA damage signaling in normal keratinocytes. Cancer Res 68(7):2214-22. [PubMed: 18381427]  [MGI Ref ID J:133302]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	This strain was generated on a mixed C57BL/6 x C3H background. It has been backcrossed 5 times to C57BL/6J and is maintained by mating hemizygous males to normal wildtype females. Expected coat color from breeding:Black and Hairless
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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