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Strain Name:

FVB/N-Tg(Trp53R172H)8512Jmr/J

Stock Number:

002659

Availability:

Repository-Cryopreserved

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Genes & Alleles   Trp53;   Wap;   Tg(Trp53R172H)8512Jmr;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator Jeffrey Rosen,   Baylor College of Medicine
GenerationN?+12

Appearance
albino
Related Genotype: Tyrc/Tyrc

Strain Description
The FVB/NTgN(Trp53R172H)8512Jmr express TRP53 with both dominant-negative and a gain-of function properties, i.e. this mutant is capable of inducing multiple drug resistance(MDR) promoter-driven reporter gene expression in transfection studies performed in p53 null cells. Transgene expression alone exerts no apparent effect on normal mammary gland development. Mice treated with the chemical carcinogen, dimethylbenz(a)anthracine (DMBA) or crossed with mice overexpressing erb-B2 develop tumors with significantly shorter latencies than controls. These tumors are characterized by large pleiomorphic nuclei and genomic instability. Spontaneous tumors are rarely observed in multiply bred animals in the first year of life.

Strain Development
0.95 kb of the 5' flanking sequence of the rat WAP (-949/+33) gene fused to a murine p53 minigene as described in Lozano, G. and Levine, A.J. Mol. Carcinog.4:3-9, 1991. Removal of the ts mutation in the original p53 minigene at codon 135(Ala-Val) and introduction of either an Arg-Leu or an Arg-His mutation at aa 172 was accomplished using recombinant PCR mutagenesis. Transgenic mice were generated by microinjection into FVB mouse embryos. (Biol. Mammary Gland, WWW)

Related Disease (OMIM) Terms

Breast Cancer
Mammalian Phenotype Terms assigned by genotype

Tg(Trp53R172H)8512Jmr/0

        involves: FVB
  • tumorigenesis
  • increased incidence of chemically-induced tumors (MGI Ref ID J:46426)
    • despite normal incidence of spontaneous tumors, mice exhibit an increase in rate of development and tumor burden of DMBA-induced mammary carcinomas compared to similarly treated wild type mice
    • DMBA induces type B mammary gland adenocarcinomas and adenomas compared to carcinomas and adenocarcinomas that develop in similarly treated wild type mice
  • mammary adenocarcinoma (MGI Ref ID J:46426)
    • despite normal incidence of spontaneous tumors, mice exhibit an increase in rate of development and tumor burden of DMBA-induced mammary carcinomas compared to similarly treated wild type mice
    • DMBA induces type B mammary gland adenocarcinomas and adenomas compared to carcinomas and adenocarcinomas that develop in similarly treated wild type mice
  • cellular phenotype
  • chromosomal instability (MGI Ref ID J:46426)
    • tumor cells exhibit an increase in genomic instability (tetraploid and aneuploid) compared to tumor cells in wild type mice
  • endocrine/exocrine gland phenotype
  • *normal* endocrine/exocrine gland phenotype (MGI Ref ID J:46426)
    • mammary gland development is normal
  • homeostasis/metabolism phenotype
  • increased incidence of chemically-induced tumors (MGI Ref ID J:46426)
    • despite normal incidence of spontaneous tumors, mice exhibit an increase in rate of development and tumor burden of DMBA-induced mammary carcinomas compared to similarly treated wild type mice
    • DMBA induces type B mammary gland adenocarcinomas and adenomas compared to carcinomas and adenocarcinomas that develop in similarly treated wild type mice

Gene & Allele Details

Allele Symbol Tg(Trp53R172H)8512Jmr
Allele Name transgene insertion 8512, Jeffrey Rosen
Common Name(s) 172R-H; p53-172H;
Mutation Made By Jeffrey Rosen,   Baylor College of Medicine
Strain of OriginFVB
Expressed Gene Trp53, transformation related protein 53, mouse, laboratory
Promoter Wap, whey acidic protein, rat
General Note On an FVB/N background transgenic mice express TRP53 with both dominant-negative and a gain-of function properties.
Molecular Note This transgene contains the rat whey acidic protein promoter and an allele of the mouse Trp53 mini-gene that carries a point mutation in codon 172 (CGC->CAC; Arg172His). [MGI Ref ID J:46426]

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(Trp53R172_)

Colony Maintenance

Breeding & HusbandryThere are no reproductive or lactational defects observed in this strain so it can be maintained by breeding either FVB/NJ females or males. Expected coat color from breeding:Albino
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Tg(Trp53R172H)8512Jmr allele
007962   B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J
View Strains carrying   Tg(Trp53R172H)8512Jmr     (1 strain)

View Strains carrying other alleles of Trp53     (11 strains)

Strains carrying other alleles of Wap
002660   FVB/N-Tg(Trp53R172L)4491Jmr/J
View Strains carrying other alleles of Wap     (1 strain)

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation

Trp53 related

Apoptosis Research
Endogenous Regulators

Cancer Research
Increased Tumor Incidence (Lymphomas)
Increased Tumor Incidence (Other Tissues/Organs: osteosarcoma)
Toxicology
Tumor Suppressor Genes

Immunology and Inflammation Research
Intracellular Signaling Molecules

Mouse/Human Gene Homologs
Li-Fraumeni syndrome

Research Tools
Toxicology Research (B and T cell deficiency) (xenograft transplant host)
Toxicology Research (drug/compound testing)

References

Selected Reference(s)

Li B; Murphy KL; Laucirica R; Kittrell F; Medina D; Rosen JM. 1998. A transgenic mouse model for mammary carcinogenesis. Oncogene 16(8):997-1007. [PubMed: 9519874]  [MGI Ref ID J:46426]

Additional References

Price and Supply Information

Strain Name: FVB/N-Tg(Trp53R172H)8512Jmr/J
Stock Number: 002659

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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