Strain Name:

FVB/N-Tg(Trp53R172H)8512Jmr/J

Stock Number:

002659

Availability:

Cryopreserved - Ready for recovery

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN?+12
 
Donating Investigator Jeffrey Rosen,   Baylor College of Medicine

Appearance
albino
Related Genotype: Tyrc/Tyrc

Description
The FVB/NTgN(Trp53R172H)8512Jmr express TRP53 with both dominant-negative and a gain-of function properties, i.e. this mutant is capable of inducing multiple drug resistance(MDR) promoter-driven reporter gene expression in transfection studies performed in p53 null cells. Transgene expression alone exerts no apparent effect on normal mammary gland development. Mice treated with the chemical carcinogen, dimethylbenz(a)anthracine (DMBA) or crossed with mice overexpressing erb-B2 develop tumors with significantly shorter latencies than controls. These tumors are characterized by large pleiomorphic nuclei and genomic instability. Spontaneous tumors are rarely observed in multiply bred animals in the first year of life.

Development
0.95 kb of the 5' flanking sequence of the rat WAP (-949/+33) gene fused to a murine p53 minigene as described in Lozano, G. and Levine, A.J. Mol. Carcinog.4:3-9, 1991. Removal of the ts mutation in the original p53 minigene at codon 135(Ala-Val) and introduction of either an Arg-Leu or an Arg-His mutation at aa 172 was accomplished using recombinant PCR mutagenesis. Transgenic mice were generated by microinjection into FVB mouse embryos. (Biol. Mammary Gland, WWW)

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Tg(Trp53R172H)8512Jmr allele
007962   B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J
View Strains carrying   Tg(Trp53R172H)8512Jmr     (1 strain)

View Strains carrying other alleles of Trp53     (20 strains)

Strains carrying other alleles of Wap
002499   C57BL/6J-Tg(WapIGFBP3)67Dlr/J
002677   FVB.Cg-Tg(WapMyc)212Bri/J
002660   FVB/N-Tg(Trp53R172L)4491Jmr/J
View Strains carrying other alleles of Wap     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(Trp53R172H)8512Jmr/0

        involves: FVB
  • tumorigenesis
  • increased incidence of chemically-induced tumors (MGI Ref ID J:46426)
    • despite normal incidence of spontaneous tumors, mice exhibit an increase in rate of development and tumor burden of DMBA-induced mammary carcinomas compared to similarly treated wild-type mice
    • DMBA induces type B mammary gland adenocarcinomas and adenomas compared to carcinomas and adenocarcinomas that develop in similarly treated wild-type mice
  • mammary adenocarcinoma (MGI Ref ID J:46426)
    • despite normal incidence of spontaneous tumors, mice exhibit an increase in rate of development and tumor burden of DMBA-induced mammary carcinomas compared to similarly treated wild-type mice
    • DMBA induces type B mammary gland adenocarcinomas and adenomas compared to carcinomas and adenocarcinomas that develop in similarly treated wild-type mice
  • cellular phenotype
  • chromosomal instability (MGI Ref ID J:46426)
    • tumor cells exhibit an increase in genomic instability (tetraploid and aneuploid) compared to tumor cells in wild-type mice
  • endocrine/exocrine gland phenotype
  • *normal* endocrine/exocrine gland phenotype (MGI Ref ID J:46426)
    • mammary gland development is normal
  • homeostasis/metabolism phenotype
  • increased incidence of chemically-induced tumors (MGI Ref ID J:46426)
    • despite normal incidence of spontaneous tumors, mice exhibit an increase in rate of development and tumor burden of DMBA-induced mammary carcinomas compared to similarly treated wild-type mice
    • DMBA induces type B mammary gland adenocarcinomas and adenomas compared to carcinomas and adenocarcinomas that develop in similarly treated wild-type mice
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation

Trp53 related

Apoptosis Research
Endogenous Regulators

Cancer Research
Increased Tumor Incidence
      Lymphomas
      Other Tissues/Organs: osteosarcoma
Toxicology
Tumor Suppressor Genes

Immunology and Inflammation Research
Intracellular Signaling Molecules

Mouse/Human Gene Homologs
Li-Fraumeni syndrome

Research Tools
Toxicology Research
      B and T cell deficiency, xenograft transplant host
      drug/compound testing

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(Trp53R172H)8512Jmr
Allele Name transgene insertion 8512, Jeffrey Rosen
Allele Type Transgenic (random, expressed)
Common Name(s) 172R-H; p53-172H;
Mutation Made By Jeffrey Rosen,   Baylor College of Medicine
Strain of OriginFVB
Expressed Gene Trp53, transformation related protein 53, mouse, laboratory
Promoter Wap, whey acidic protein, rat
General Note On an FVB/N background transgenic mice express TRP53 with both dominant-negative and a gain-of function properties.
Molecular Note This transgene contains the rat whey acidic protein promoter and an allele of the mouse Trp53 mini-gene that carries a point mutation in codon 172 (CGC->CAC; Arg172His). [MGI Ref ID J:46426]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Trp53R172_), Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Li B; Murphy KL; Laucirica R; Kittrell F; Medina D; Rosen JM. 1998. A transgenic mouse model for mammary carcinogenesis. Oncogene 16(8):997-1007. [PubMed: 9519874]  [MGI Ref ID J:46426]

Additional References

Li B; Greenberg N; Stephens LC; Meyn R; Medina D; Rosen JM. 1994. Preferential overexpression of a 172Arg-->Leu mutant p53 in the mammary gland of transgenic mice results in altered lobuloalveolar development. Cell Growth Differ 5(7):711-21. [PubMed: 7947386]  [MGI Ref ID J:56433]

Tg(Trp53R172H)8512Jmr related

Chatterjee G; Rosner A; Han Y; Zelazny ET; Li B; Cardiff RD; Perkins AS. 2002. Acceleration of Mouse Mammary Tumor Virus-Induced Murine Mammary Tumorigenesis by a p53(172H) Transgene: Influence of FVB Background on Tumor Latency and Identification of Novel Sites of Proviral Insertion. Am J Pathol 161(6):2241-53. [PubMed: 12466138]  [MGI Ref ID J:80272]

Hadsell DL; Murphy KL; Bonnette SG; Reece N; Laucirica R; Rosen JM. 2000. Cooperative interaction between mutant p53 and des(1-3)IGF-I accelerates mammary tumorigenesis. Oncogene 19(7):889-98. [PubMed: 10702797]  [MGI Ref ID J:61037]

Li B; Rosen JM; McMenamin-Balano J; Muller WJ; Perkins AS. 1997. neu/ERBB2 cooperates with p53-172H during mammary tumorigenesis in transgenic mice. Mol Cell Biol 17(6):3155-63. [PubMed: 9154814]  [MGI Ref ID J:40336]

Pannellini T; Spadaro M; Di Carlo E; Ambrosino E; Iezzi M; Amici A; Lollini PL; Forni G; Cavallo F; Musiani P. 2006. Timely DNA vaccine combined with systemic IL-12 prevents parotid carcinomas before a dominant-negative p53 makes their growth independent of HER-2/neu expression. J Immunol 176(12):7695-703. [PubMed: 16751417]  [MGI Ref ID J:115041]

Wall EM; Milne K; Martin ML; Watson PH; Theiss P; Nelson BH. 2007. Spontaneous mammary tumors differ widely in their inherent sensitivity to adoptively transferred T cells. Cancer Res 67(13):6442-50. [PubMed: 17616705]  [MGI Ref ID J:122846]

Zelazny E; Li B; Anagnostopoulos AM; Coleman A; Perkins AS. 2001. Cooperating Oncogenic Events in Murine Mammary Tumorigenesis: Assessment of ErbB2, Mutant p53, and Mouse Mammary Tumor Virus. Exp Mol Pathol 70(3):183-93. [PubMed: 11417997]  [MGI Ref ID J:70644]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & HusbandryThere are no reproductive or lactational defects observed in this strain so it can be maintained by breeding either FVB/NJ females or males. Expected coat color from breeding:Albino
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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