Strain Name:

FVB/N-Tg(Trp53R172L)4491Jmr/J

Stock Number:

002660

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Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Jeffrey Rosen,   Baylor College of Medicine

Description
The FVB/N-TgN(Trp53R172L)4491Jmr express TRP53 with pseudo-wildtype properties capable of inducing p21 and mdm-2 expression. Lobuloalveolar development is altered and apoptosis is increased during late pregnancy. The few normal lobules observed during early lactation did not express the Trp53 transgene suggesting that they arose by clonal expansion of cells not expressing the transgene during mid-pregnancy. Transgenic mice fail to lactate. There is no apparent alteration in ductal development. Mice expressing a dominant negative 172Arg-His mutation do not exhibit any detectable alterations in mammary gland development and have a very low incidence of spontaneous mammary tumors. Mice bearing the 172Arg-Leu transgene and a pituitary isograft displayed a marked increase in apoptosis and a significant delay in DMBA-induced tumorigenesis, while those bearing the 172Arg-His dominant negative p53 transgene were more susceptible to DMBA-induced tumorigenesis.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Trp53     (27 strains)

Strains carrying other alleles of Wap
002499   C57BL/6J-Tg(WapIGFBP3)67Dlr/J
002677   FVB.Cg-Tg(WapMyc)212Bri/J
002659   FVB/N-Tg(Trp53R172H)8512Jmr/J
View Strains carrying other alleles of Wap     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Adrenocortical Carcinoma, Hereditary; ADCC   (TP53)
Basal Cell Carcinoma, Susceptibility to, 7; BCC7   (TP53)
Breast Cancer   (TP53)
Colorectal Cancer; CRC   (TP53)
Glioma Susceptibility 1; GLM1   (TP53)
Hepatocellular Carcinoma   (TP53)
Li-Fraumeni Syndrome 1; LFS1   (TP53)
Nasopharyngeal Carcinoma   (TP53)
Osteogenic Sarcoma   (TP53)
Pancreatic Cancer   (TP53)
Papilloma of Choroid Plexus; CPP   (TP53)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(Trp53R172L)4491Jmr/0

        FVB/N-Tg(Trp53R172L)4491Jmr
  • tumorigenesis
  • *normal* tumorigenesis
    • despite expression of the transgene, mice do not exhibit an increase in spontaneous tumor rates   (MGI Ref ID J:56433)
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland growth during lactation
    • during lactation, mammary glands exhibit incomplete development with few normal lobuloalveolar structures compared to in wild-type mice   (MGI Ref ID J:56433)
  • abnormal mammary gland physiology
    • mice exhibit increased mammary gland cell sensitivity to radiation induced apoptosis compared to in similarly treated wild-type mice   (MGI Ref ID J:56433)
    • lactation failure
      • females fail to lactate resulting in reduced pup weight and increased pup mortality   (MGI Ref ID J:56433)
  • integument phenotype
  • abnormal hair follicle morphology
    • mice exhibit follicular dilatation and comedo formation   (MGI Ref ID J:56433)
  • abnormal mammary gland growth during lactation
    • during lactation, mammary glands exhibit incomplete development with few normal lobuloalveolar structures compared to in wild-type mice   (MGI Ref ID J:56433)
  • abnormal mammary gland physiology
    • mice exhibit increased mammary gland cell sensitivity to radiation induced apoptosis compared to in similarly treated wild-type mice   (MGI Ref ID J:56433)
    • lactation failure
      • females fail to lactate resulting in reduced pup weight and increased pup mortality   (MGI Ref ID J:56433)
  • skin lesions
    • lesions in the hind quarter represent follicular dilatation and comedo formation   (MGI Ref ID J:56433)

Tg(Trp53R172L)4491Jmr/0

        involves: FVB/N
  • tumorigenesis
  • decreased incidence of tumors by chemical induction
    • following pituitary engrafts and exposure to DMBA, mice exhibit reduced induction rates and tumor burden of mammary tumors compared to similarly treated wild-type mice   (MGI Ref ID J:101547)
    • both squamous adenocarcinomas (early developing) and type B mammary adenocarcinomas (late developing) are induced by DMBA treatment of mice with pituitary engrafts unlike in similarly treated wild-type mice, which mainly develop squamous adenocarcinomas   (MGI Ref ID J:101547)
    • however, DMBA treated mice that do not receive pituitary engrafts exhibit normal tumorigenesis   (MGI Ref ID J:101547)
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland growth during lactation
    • by day 2 of lactation following gamma irradiation, few normal lobuloalveolar structures are detected unlike in wild-type mice due to increased apoptosis   (MGI Ref ID J:101547)
    • pituitary engrafts increase apoptosis by 10-fold and the disruption of lobuloalveolar structures observed during lactation   (MGI Ref ID J:101547)
  • integument phenotype
  • abnormal mammary gland growth during lactation
    • by day 2 of lactation following gamma irradiation, few normal lobuloalveolar structures are detected unlike in wild-type mice due to increased apoptosis   (MGI Ref ID J:101547)
    • pituitary engrafts increase apoptosis by 10-fold and the disruption of lobuloalveolar structures observed during lactation   (MGI Ref ID J:101547)
  • homeostasis/metabolism phenotype
  • decreased incidence of tumors by chemical induction
    • following pituitary engrafts and exposure to DMBA, mice exhibit reduced induction rates and tumor burden of mammary tumors compared to similarly treated wild-type mice   (MGI Ref ID J:101547)
    • both squamous adenocarcinomas (early developing) and type B mammary adenocarcinomas (late developing) are induced by DMBA treatment of mice with pituitary engrafts unlike in similarly treated wild-type mice, which mainly develop squamous adenocarcinomas   (MGI Ref ID J:101547)
    • however, DMBA treated mice that do not receive pituitary engrafts exhibit normal tumorigenesis   (MGI Ref ID J:101547)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(Trp53R172L)4491Jmr
Allele Name transgene insertion 4491, Jeffrey Rosen
Allele Type Transgenic (Inserted expressed sequence)
Mutation Made ByDr. Jeffrey Rosen,   Baylor College of Medicine
Strain of OriginFVB
Expressed Gene Trp53, transformation related protein 53, mouse, laboratory
Promoter Wap, whey acidic protein, rat
Molecular Note This transgene contains the rat whey acidic protein promoter (0.95 kb of 5' sequence) fused to a mouse Trp53 minigene that carries a transversion in codon 172 (CGC->CTC; "Arg172Leu"). Removal of the ts mutation in the original Trp53 minigene at codon 135(Ala-Val) and introduction of the Arg-Leu mutation at amino acid 172 were accomplished using recombinant PCR mutagenesis. [MGI Ref ID J:56433]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Trp53R172_), Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Li B; Greenberg N; Stephens LC; Meyn R; Medina D; Rosen JM. 1994. Preferential overexpression of a 172Arg-->Leu mutant p53 in the mammary gland of transgenic mice results in altered lobuloalveolar development. Cell Growth Differ 5(7):711-21. [PubMed: 7947386]  [MGI Ref ID J:56433]

Additional References

Tg(Trp53R172L)4491Jmr related

Li B; Kittrell FS; Medina D; Rosen JM. 1995. Delay of dimethylbenz[a]anthracene-induced mammary tumorigenesis in transgenic mice by apoptosis induced by an unusual mutant p53 protein. Mol Carcinog 14(2):75-83. [PubMed: 7576102]  [MGI Ref ID J:101547]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3300.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4290.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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