Strain Name:

FVB/N-Tg(Trp53R172L)4491Jmr/J

Stock Number:

002660

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Purchasing Information

Description

Strain Information

Type Mutant Strain; Transgenic;
Specieslaboratory mouse
GenerationN?+10
 
Donating Investigator Jeffrey Rosen,   Baylor College of Medicine

Description
The FVB/N-TgN(Trp53R172L)4491Jmr express TRP53 with pseudo-wildtype properties capable of inducing p21 and mdm-2 expression. Lobuloalveolar development is altered and apoptosis is increased during late pregnancy. The few normal lobules observed during early lactation did not express the Trp53 transgene suggesting that they arose by clonal expansion of cells not expressing the transgene during mid-pregnancy. Transgenic mice fail to lactate. There is no apparent alteration in ductal development. Mice expressing a dominant negative 172Arg-His mutation do not exhibit any detectable alterations in mammary gland development and have a very low incidence of spontaneous mammary tumors. Mice bearing the 172Arg-Leu transgene and a pituitary isograft displayed a marked increase in apoptosis and a significant delay in DMBA-induced tumorigenesis, while those bearing the 172Arg-His dominant negative p53 transgene were more susceptible to DMBA-induced tumorigenesis.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Trp53     (13 strains)

View Strains carrying other alleles of Wap     (3 strains)

Phenotype

Phenotype Information

Mammalian Phenotype Terms assigned by genotype

Tg(Trp53R172L)4491Jmr/0

        FVB/N-Tg(Trp53R172L)4491Jmr
  • reproductive system phenotype
  • abnormal mammary gland growth during lactation (MGI Ref ID J:56433)
    • during lactation, mammary glands exhibit incomplete development with few normal lobuloalveolar structures compared to in wild-type mice
  • abnormal mammary gland physiology (MGI Ref ID J:56433)
    • mice exhibit increased mammary gland cell sensitivity to radiation induced apoptosis compared to in similarly treated wild-type mice
    • abnormal lactation (MGI Ref ID J:56433)
      • females fail to lactate resulting in reduced pup weight and increased pup mortality
  • skin/coat/nails phenotype
  • abnormal hair follicle morphology (MGI Ref ID J:56433)
    • mice exhibit follicular dilatation and comedo formation
  • skin lesions (MGI Ref ID J:56433)
    • lesions in the hind quarter represent follicular dilatation and comedo formation
  • tumorigenesis
  • *normal* tumorigenesis (MGI Ref ID J:56433)
    • despite expression of the transgene, mice do not exhibit an increase in spontaneous tumor rates
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland growth during lactation (MGI Ref ID J:56433)
    • during lactation, mammary glands exhibit incomplete development with few normal lobuloalveolar structures compared to in wild-type mice
  • abnormal mammary gland physiology (MGI Ref ID J:56433)
    • mice exhibit increased mammary gland cell sensitivity to radiation induced apoptosis compared to in similarly treated wild-type mice
    • abnormal lactation (MGI Ref ID J:56433)
      • females fail to lactate resulting in reduced pup weight and increased pup mortality

Tg(Trp53R172L)4491Jmr/0

        involves: FVB/N
  • reproductive system phenotype
  • abnormal mammary gland growth during lactation (MGI Ref ID J:101547)
    • by day 2 of lactation following gamma irradiation, few normal lobuloalveolar structures are detected unlike in wild-type mice due to increased apoptosis
    • pituitary engrafts increase apoptosis by 10-fold and the disruption of lobuloalveolar structures observed during lactation
  • tumorigenesis
  • decreased incidence of chemically-induced tumors (MGI Ref ID J:101547)
    • following pituitary engrafts and exposure to DMBA, mice exhibit reduced induction rates and tumor burden of mammary tumors compared to similarly treated wild-type mice
    • both squamous adenocarcinomas (early developing) and type B mammary adenocarcinomas (late developing) are induced by DMBA treatment of mice with pituitary engrafts unlike in similarly treated wild-type mice, which mainly develop squamous adenocarcinomas
    • however, DMBA treated mice that do not receive pituitary engrafts exhibit normal tumorigenesis
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland growth during lactation (MGI Ref ID J:101547)
    • by day 2 of lactation following gamma irradiation, few normal lobuloalveolar structures are detected unlike in wild-type mice due to increased apoptosis
    • pituitary engrafts increase apoptosis by 10-fold and the disruption of lobuloalveolar structures observed during lactation

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Genes Regulating Growth and Proliferation

Trp53 related

Apoptosis Research
Endogenous Regulators

Cancer Research
Increased Tumor Incidence (Lymphomas)
Increased Tumor Incidence (Other Tissues/Organs: osteosarcoma)
Toxicology
Tumor Suppressor Genes

Immunology and Inflammation Research
Intracellular Signaling Molecules

Mouse/Human Gene Homologs
Li-Fraumeni syndrome

Research Tools
Toxicology Research (B and T cell deficiency) (xenograft transplant host)
Toxicology Research (drug/compound testing)

Genes & Alleles

Gene & Allele Information

Allele Symbol Tg(Trp53R172L)4491Jmr
Allele Name transgene insertion 4491, Jeffrey Rosen
Mutation Made By Jeffrey Rosen,   Baylor College of Medicine
Strain of OriginFVB
Expressed Gene Trp53, transformation related protein 53, mouse, laboratory
Promoter Wap, whey acidic protein, rat
Molecular Note This transgene contains the rat whey acidic protein promoter (0.95 kb of 5' sequence) fused to a mouse Trp53 minigene that carries a transversion in codon 172 (CGC->CTC; "Arg172Leu"). Removal of the ts mutation in the original Trp53 minigene at codon 135(Ala-Val) and introduction of the Arg-Leu mutation at amino acid 172 were accomplished using recombinant PCR mutagenesis. [MGI Ref ID J:56433]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(Trp53R172_), STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Li B; Greenberg N; Stephens LC; Meyn R; Medina D; Rosen JM. 1994. Preferential overexpression of a 172Arg-->Leu mutant p53 in the mammary gland of transgenic mice results in altered lobuloalveolar development. Cell Growth Differ 5(7):711-21. [PubMed: 7947386]  [MGI Ref ID J:56433]

Additional References

Tg(Trp53R172L)4491Jmr related

Li B; Kittrell FS; Medina D; Rosen JM. 1995. Delay of dimethylbenz[a]anthracene-induced mammary tumorigenesis in transgenic mice by apoptosis induced by an unusual mutant p53 protein. Mol Carcinog 14(2):75-83. [PubMed: 7576102]  [MGI Ref ID J:101547]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations             View   International   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $1900.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Pricing for International shipping destinations             View   USA, Canada and Mexico   Pricing
Weeks of AgePrice*Gender
Cryorecovery Fee $2470.00
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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