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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+10
Generation DefinitionsDonating Investigator Dr. Jeffrey Rosen, Baylor College of Medicine Description
The FVB/N-TgN(Trp53R172L)4491Jmr express TRP53 with pseudo-wildtype properties capable of inducing p21 and mdm-2 expression. Lobuloalveolar development is altered and apoptosis is increased during late pregnancy. The few normal lobules observed during early lactation did not express the Trp53 transgene suggesting that they arose by clonal expansion of cells not expressing the transgene during mid-pregnancy. Transgenic mice fail to lactate. There is no apparent alteration in ductal development. Mice expressing a dominant negative 172Arg-His mutation do not exhibit any detectable alterations in mammary gland development and have a very low incidence of spontaneous mammary tumors. Mice bearing the 172Arg-Leu transgene and a pituitary isograft displayed a marked increase in apoptosis and a significant delay in DMBA-induced tumorigenesis, while those bearing the 172Arg-His dominant negative p53 transgene were more susceptible to DMBA-induced tumorigenesis.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Trp53
004301 129-Trp53tm1Holl/J 002080 129-Trp53tm1Tyj/J 008652 129S-Trp53tm2Tyj/J 008651 129S-Trp53tm3Tyj/J 008462 B6.129P2-Trp53tm1Brn/J 002101 B6.129S2-Trp53tm1Tyj/J 008183 B6.129S4(Cg)-Trp53tm2.1Tyj/J 008182 B6.129S4-Trp53tm3.1Tyj/J 007218 B6.129S6-Trp53tm2Xu/J 011109 B6.Cg-Trp53tm2Glo/Kvm 018285 B6.Cg-Tyrc-2J Trp53tm1Tyj Hrhr/J 007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 017767 B6;129-Trp53tm1.1Dgk/J 008045 B6;129-Trp53tm2Holl/J 006980 B6;129-Trp53tm2Xu/J 008191 B6;129S2-Trp53tm1Tyj Nf1tm1Tyj/J 002103 B6;129S2-Trp53tm1Tyj/J 008181 B6;129S4-Trp53tm4Tyj/J 008361 B6;129S4-Trp53tm5Tyj/J 002526 C.129S2(B6)-Trp53tm1Tyj/J 002547 C3Ou.129S2(B6)-Trp53tm1Tyj/J 002899 FVB.129S2(B6)-Trp53tm1Tyj/J 002659 FVB/N-Tg(Trp53R172H)8512Jmr/J 017530 STOCK Iis2tm2(ACTB-tdTomato,-EGFP)Luo Trp53tm1Tyj Nf1tm1Par/J 012620 STOCK Trp53tm1Brd Brca1tm1Aash Tg(LGB-cre)74Acl/J 003262 STOCK Tg(Trp53A135V)L3Ber/J View Strains carrying other alleles of Trp53 (26 strains)
007962 B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J 002499 C57BL/6J-Tg(WapIGFBP3)67Dlr/J 002677 FVB.Cg-Tg(WapMyc)212Bri/J 002659 FVB/N-Tg(Trp53R172H)8512Jmr/J
Phenotype
Phenotype Information
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Adrenocortical Carcinoma, Hereditary; ADCC (TP53)
Basal Cell Carcinoma, Susceptibility to, 7; BCC7 (TP53)
Breast Cancer (TP53)
Colorectal Cancer; CRC (TP53)
Glioma Susceptibility 1; GLM1 (TP53)
Hepatocellular Carcinoma (TP53)
Li-Fraumeni Syndrome 1; LFS1 (TP53)
Nasopharyngeal Carcinoma (TP53)
Osteogenic Sarcoma (TP53)
Pancreatic Cancer (TP53)
Papilloma of Choroid Plexus (TP53)
assigned by genotype
Tg(Trp53R172L)4491Jmr/0
FVB/N-Tg(Trp53R172L)4491Jmr
- tumorigenesis
- *normal* tumorigenesis
- despite expression of the transgene, mice do not exhibit an increase in spontaneous tumor rates (MGI Ref ID J:56433)
- endocrine/exocrine gland phenotype
- abnormal mammary gland growth during lactation
- during lactation, mammary glands exhibit incomplete development with few normal lobuloalveolar structures compared to in wild-type mice (MGI Ref ID J:56433)
- abnormal mammary gland physiology
- mice exhibit increased mammary gland cell sensitivity to radiation induced apoptosis compared to in similarly treated wild-type mice (MGI Ref ID J:56433)
- lactation failure
- females fail to lactate resulting in reduced pup weight and increased pup mortality (MGI Ref ID J:56433)
- integument phenotype
- abnormal hair follicle morphology
- mice exhibit follicular dilatation and comedo formation (MGI Ref ID J:56433)
- abnormal mammary gland growth during lactation
- during lactation, mammary glands exhibit incomplete development with few normal lobuloalveolar structures compared to in wild-type mice (MGI Ref ID J:56433)
- abnormal mammary gland physiology
- mice exhibit increased mammary gland cell sensitivity to radiation induced apoptosis compared to in similarly treated wild-type mice (MGI Ref ID J:56433)
- lactation failure
- females fail to lactate resulting in reduced pup weight and increased pup mortality (MGI Ref ID J:56433)
- skin lesions
- lesions in the hind quarter represent follicular dilatation and comedo formation (MGI Ref ID J:56433)
Tg(Trp53R172L)4491Jmr/0
involves: FVB/N
- tumorigenesis
- decreased incidence of chemically-induced tumors
- following pituitary engrafts and exposure to DMBA, mice exhibit reduced induction rates and tumor burden of mammary tumors compared to similarly treated wild-type mice (MGI Ref ID J:101547)
- both squamous adenocarcinomas (early developing) and type B mammary adenocarcinomas (late developing) are induced by DMBA treatment of mice with pituitary engrafts unlike in similarly treated wild-type mice, which mainly develop squamous adenocarcinomas (MGI Ref ID J:101547)
- however, DMBA treated mice that do not receive pituitary engrafts exhibit normal tumorigenesis (MGI Ref ID J:101547)
- endocrine/exocrine gland phenotype
- abnormal mammary gland growth during lactation
- by day 2 of lactation following gamma irradiation, few normal lobuloalveolar structures are detected unlike in wild-type mice due to increased apoptosis (MGI Ref ID J:101547)
- pituitary engrafts increase apoptosis by 10-fold and the disruption of lobuloalveolar structures observed during lactation (MGI Ref ID J:101547)
- integument phenotype
- abnormal mammary gland growth during lactation
- by day 2 of lactation following gamma irradiation, few normal lobuloalveolar structures are detected unlike in wild-type mice due to increased apoptosis (MGI Ref ID J:101547)
- pituitary engrafts increase apoptosis by 10-fold and the disruption of lobuloalveolar structures observed during lactation (MGI Ref ID J:101547)
- homeostasis/metabolism phenotype
- decreased incidence of chemically-induced tumors
- following pituitary engrafts and exposure to DMBA, mice exhibit reduced induction rates and tumor burden of mammary tumors compared to similarly treated wild-type mice (MGI Ref ID J:101547)
- both squamous adenocarcinomas (early developing) and type B mammary adenocarcinomas (late developing) are induced by DMBA treatment of mice with pituitary engrafts unlike in similarly treated wild-type mice, which mainly develop squamous adenocarcinomas (MGI Ref ID J:101547)
- however, DMBA treated mice that do not receive pituitary engrafts exhibit normal tumorigenesis (MGI Ref ID J:101547)
This mouse can be used to support research in many areas including:
Cancer Research
Genes Regulating Growth and Proliferation
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(Trp53R172L)4491Jmr | ||
|---|---|---|---|
| Allele Name | transgene insertion 4491, Jeffrey Rosen | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Dr. Jeffrey Rosen, Baylor College of Medicine | ||
| Strain of Origin | FVB | ||
| Expressed Gene | Trp53, transformation related protein 53, mouse, laboratory | ||
| Promoter | Wap, whey acidic protein, rat | ||
| Molecular Note | This transgene contains the rat whey acidic protein promoter (0.95 kb of 5' sequence) fused to a mouse Trp53 minigene that carries a transversion in codon 172 (CGC->CTC; "Arg172Leu"). Removal of the ts mutation in the original Trp53 minigene at codon 135(Ala-Val) and introduction of the Arg-Leu mutation at amino acid 172 were accomplished using recombinant PCR mutagenesis. [MGI Ref ID J:56433] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(Trp53R172_), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Li B; Greenberg N; Stephens LC; Meyn R; Medina D; Rosen JM. 1994. Preferential overexpression of a 172Arg-->Leu mutant p53 in the mammary gland of transgenic mice results in altered lobuloalveolar development. Cell Growth Differ 5(7):711-21. [PubMed: 7947386] [MGI Ref ID J:56433]
Additional References
Tg(Trp53R172L)4491Jmr relatedLi B; Kittrell FS; Medina D; Rosen JM. 1995. Delay of dimethylbenz[a]anthracene-induced mammary tumorigenesis in transgenic mice by apoptosis induced by an unusual mutant p53 protein. Mol Carcinog 14(2):75-83. [PubMed: 7576102] [MGI Ref ID J:101547]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3175.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $4127.50 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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