Strain Name: |
B6;SJL-Tg(EPO33)72Ptc/J |
|---|---|
Stock Number: |
002661 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | EPO; Ucp; Tg(EPO33)72Ptc; |
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Peter Curtin, New England Medical Center Generation ?pN1 Strain Description
Homozygotes are viable and fertile. The transgene in line Epo33-72 encompasses a 33 kb genomic fragment including 4 kb of human EPO along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Anemia produced by phelebotomy or treatment with phenylhydrazine induces the transgene in kidney and liver with some variability. Cobalt and hypoxia also reportedly induce the transgene. Brain, heart, kidney, spleen and liver were tested in animals which did not have induced anemia and constitutive expression was not found.
Mammalian Phenotype Terms assigned by genotype |
| Allele Symbol | Tg(EPO33)72Ptc | ||
|---|---|---|---|
| Allele Name | transgene insertion 72, Peter Curtin | ||
| Mutation Made By | Peter Curtin, New England Medical Center | ||
| Strain of Origin | C57BL/6J x SJL/J | ||
| Expressed Gene | EPO, erythropoietin, human | ||
| Promoter | Ucp, mitochondrial uncoupling protein, | ||
| General Note | Anemia produced by phelebotomy or treatment with phenylhydrazine induced the transgene in kidney and liver with some variability; cobalt and hypoxia also induced the transgene. In the absence of induced anemia, constitutive expression was not found in brain, heart, kidney, spleen and liver. | ||
| Molecular Note | The transgene contains a mitochondrial uncoupling protein promoter (Ucp) and a 33 kb genomic fragment including 4 kb of human erythropoietin (EPO) along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Several lines were created.Gene copy number in line 72 is 2. [MGI Ref ID J:92650] | ||
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| 000686 SJL/J | (approximate) | |
| Considerations for Choosing Controls | ||
Tg(EPO33)72Ptc
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Strains carrying other alleles of Ucp
002384 FVB/N-Tg(UcpDta)1Kz/J View Strains carrying other alleles of Ucp (1 strain)
EPO related
Hematological Research
Anemia, Iron Deficiency and Transport Defects
Selected Reference(s)
Madan A; Lin C; Hatch SL 2nd; Curtin PT. 1995. Regulated basal, inducible, and tissue-specific human erythropoietin gene expression in transgenic mice requires multiple cis DNA sequences. Blood 85(10):2735-41. [PubMed: 7742534] [MGI Ref ID J:92650]
| Strain Name: | B6;SJL-Tg(EPO33)72Ptc/J |
| Stock Number: | 002661 |
IMPORTANT NOTE: Prices are based on shipping destination. To view prices, select your shipping destination.
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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