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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
- Terms of use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation ?pN1
Generation DefinitionsDonating Investigator Dr. Peter Curtin, New England Medical Center Description
Homozygotes are viable and fertile. The transgene in line Epo33-72 encompasses a 33 kb genomic fragment including 4 kb of human EPO along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Anemia produced by phelebotomy or treatment with phenylhydrazine induces the transgene in kidney and liver with some variability. Cobalt and hypoxia also reportedly induce the transgene. Brain, heart, kidney, spleen and liver were tested in animals which did not have induced anemia and constitutive expression was not found.
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| 000686 SJL/J | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Ucp
002384 FVB/N-Tg(UcpDta)1Kz/J View Strains carrying other alleles of Ucp (1 strain)
Phenotype
Phenotype Information
- Potential model based on transgenic expression of an ortholog of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested. Microvascular Complications of Diabetes, Susceptibility to, 2; MVCD2 (EPO)
assigned by genotype
Tg(EPO33)72Ptc/0
involves: C57BL/6J * SJL/J
- mortality/aging
- *normal* mortality/aging
- despite an increase in hematocrit, mice exhibit a normal life span (MGI Ref ID J:92650)
- hematopoietic system phenotype
- enlarged spleen (MGI Ref ID J:92650)
- increased hematocrit
- mice exhibit polycythemia and erythroid hyperplasia in the marrow and spleen (MGI Ref ID J:92650)
- immune system phenotype
- enlarged spleen (MGI Ref ID J:92650)
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Tg(EPO33)72Ptc | ||
|---|---|---|---|
| Allele Name | transgene insertion 72, Peter Curtin | ||
| Allele Type | Transgenic (random, expressed) | ||
| Mutation Made By | Dr. Peter Curtin, New England Medical Center | ||
| Strain of Origin | C57BL/6J x SJL/J | ||
| Expressed Gene | EPO, erythropoietin, human | ||
| Promoter | Ucp, mitochondrial uncoupling protein, | ||
| General Note | Anemia produced by phelebotomy or treatment with phenylhydrazine induced the transgene in kidney and liver with some variability; cobalt and hypoxia also induced the transgene. In the absence of induced anemia, constitutive expression was not found in brain, heart, kidney, spleen and liver. | ||
| Molecular Note | The transgene contains a mitochondrial uncoupling protein promoter (Ucp) and a 33 kb genomic fragment including 4 kb of human erythropoietin (EPO) along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Several lines were created.Gene copy number in line 72 is 2. [MGI Ref ID J:92650] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(EPO33)72Ptc, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References provided by MGI
Selected Reference(s)
Madan A; Lin C; Hatch SL 2nd; Curtin PT. 1995. Regulated basal, inducible, and tissue-specific human erythropoietin gene expression in transgenic mice requires multiple cis DNA sequences. Blood 85(10):2735-41. [PubMed: 7742534] [MGI Ref ID J:92650]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3000.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
| Pricing for International shipping destinations |
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Cryopreserved
Cryopreserved Mice - Ready for Recovery
Animals Provided
Price (US dollars $) Cryorecovery* $3900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
Supply Notes
- Cryorecovery - Standard.
Progeny testing is not required.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).
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Standard Supply
Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.
General Supply Notes
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| 000686 SJL/J | (approximate) | |
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
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See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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Contact information
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| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
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