Strain Name: |
B6;SJL-Tg(EPO33)72Ptc/J |
|---|---|
Stock Number: |
002661 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | EPO; Ucp; Tg(EPO33)72Ptc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Transgenic Species laboratory mouse Donating Investigator Peter Curtin, New England Medical Center Generation ?pN1 Strain Description
Homozygotes are viable and fertile. The transgene in line Epo33-72 encompasses a 33 kb genomic fragment including 4 kb of human EPO along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Anemia produced by phelebotomy or treatment with phenylhydrazine induces the transgene in kidney and liver with some variability. Cobalt and hypoxia also reportedly induce the transgene. Brain, heart, kidney, spleen and liver were tested in animals which did not have induced anemia and constitutive expression was not found.
Mammalian Phenotype Terms assigned by genotype |
Gene & Allele Details
| Allele Symbol | Tg(EPO33)72Ptc | ||
|---|---|---|---|
| Allele Name | transgene insertion 72, Peter Curtin | ||
| Mutation Made By | Peter Curtin, New England Medical Center | ||
| Strain of Origin | C57BL/6J x SJL/J | ||
| Expressed Gene | EPO, erythropoietin, human | ||
| Promoter | Ucp, mitochondrial uncoupling protein, | ||
| General Note | Anemia produced by phelebotomy or treatment with phenylhydrazine induced the transgene in kidney and liver with some variability; cobalt and hypoxia also induced the transgene. In the absence of induced anemia, constitutive expression was not found in brain, heart, kidney, spleen and liver. | ||
| Molecular Note | The transgene contains a mitochondrial uncoupling protein promoter (Ucp) and a 33 kb genomic fragment including 4 kb of human erythropoietin (EPO) along with 20 kb of 5' flanking sequence and 8.5 kb of 3' flanking sequence. Several lines were created.Gene copy number in line 72 is 2. [MGI Ref ID J:92650] | ||
Control Information
| Control | ||
|---|---|---|
| 100012 B6SJLF1/J | (approximate) | |
| 000664 C57BL/6J | (approximate) | |
| 000686 SJL/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Tg(EPO33)72Ptc
Colony Maintenance
| Diet Information | LabDiet® 5K52/5K67 |
|---|
Related Strains
Strains carrying other alleles of Ucp
002384 FVB/N-Tg(UcpDta)1Kz/J View Strains carrying other alleles of Ucp (1 strain)
Research Applications
This mouse can be used to support research in many areas including:EPO related
Hematological Research
Anemia, Iron Deficiency and Transport Defects
References
Selected Reference(s)
Madan A; Lin C; Hatch SL 2nd; Curtin PT. 1995. Regulated basal, inducible, and tissue-specific human erythropoietin gene expression in transgenic mice requires multiple cis DNA sequences. Blood 85(10):2735-41. [PubMed: 7742534] [MGI Ref ID J:92650]
Price and Supply Information
| Strain Name: | B6;SJL-Tg(EPO33)72Ptc/J |
| Stock Number: | 002661 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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