Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S2 via D3 ES cell line Generation N8+N1F8 (20-DEC-06)   Donating Investigator Tak Mak,   University Health Network/Un of Toronto

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Cd4^tm1Mak targeted mutation have a significant block in CD4⁺ T-cell development; 90% of their circulating T-cells are CD8⁺. Cell surface expression of CD4 protein is not detected on thymocytes and lymph node cells from homozygous mice. Homozygous mutant mice also show a Class II restricted deficit in helper T-cell activity and other T-cell responses. This mutant mouse strain may be useful in studies of T cell development, susceptibility to viral infection and inflammation.

Development
A targeting vector containing a PGK-neo cassette was used to disrupt exon 5. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The mice were crossed to C57BL/6 mice for 8 geneartions.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Cd4^tm1Mak allele

002664	B6;129S-Cd4tm1Mak Cd8atm1Mak/J
006483	CBy.129S2(B6)-Cd4tm1Mak/J

View Strains carrying   Cd4^tm1Mak     (2 strains)

Strains carrying other alleles of Cd4

002447	B10.129S2(B6)-Cd4tm1Litt/J
002269	B6.129S6-Cd4tm1Knw/J
002268	B6;129S6-Cd4tm1Knw/J
003090	NOD.129S6(B6)-Cd4tm1Knw/DvsJ
005328	NOD/ShiLt-Tg(Cd4-DsRed)4Lt/J

View Strains carrying other alleles of Cd4     (5 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cd4^tm1Mak/Cd4^tm1Mak

        B6.129S2-Cd4^tm1Mak/J

• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:133047)
• absent plasma cells (MGI Ref ID J:133047)
• hematopoietic system phenotype
• absent plasma cells (MGI Ref ID J:133047)
• homeostasis/metabolism phenotype
• abnormal bile salt level (MGI Ref ID J:133047)

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd4^tm1Mak/Cd4^tm1Mak

        involves: 129S2/SvPas * C57BL/6 * DBA/2

• hematopoietic system phenotype
• increased CD8-positive T cell number (MGI Ref ID J:68957)
  • peripheral CD8+ cell population is expanded
• immune system phenotype
• abnormal CD4-positive T cell physiology (MGI Ref ID J:68957)
  • no response to class II alloantigens, but normal class I
• increased CD8-positive T cell number (MGI Ref ID J:68957)
  • peripheral CD8+ cell population is expanded
• increased susceptibility to viral infection (MGI Ref ID J:92227)
  • significantly increased inflammation in the first week of TMEV infection which is mostly resolved by 45 days after infection on the resistant C57BL/6 background
  • persistent infectious virus at 45 days and increasing clinical symptoms over time
  • at 10 months, 93% of mice present clinical symptoms on the resistant C57BL/6 background
• nervous system phenotype
• demyelination (MGI Ref ID J:92227)
  • on the resistant C57BL/6 background, demyelination was light at 45 days after TMEV infection, but extensive at 90 days

Cd4^tm1Mak/Cd4^tm1Mak

        P/J

• immune system phenotype
• brain inflammation (MGI Ref ID J:92227)
  • severely increased meningeal inflammation on both PL/J and SJL/J backgrounds after infection with TMEV
• increased susceptibility to viral infection (MGI Ref ID J:92227)
  • inflammation persists in the susceptible PL/J background, particularly in the cerebellum, brainstem and striatum
• nervous system phenotype
• brain inflammation (MGI Ref ID J:92227)
  • severely increased meningeal inflammation on both PL/J and SJL/J backgrounds after infection with TMEV
• demyelination (MGI Ref ID J:92227)
  • demyelination was severe on both the susceptible PL/J and SJL/J backgrounds after infection with TMEV

Cd4^tm1Mak/Cd4^tm1Mak

        SJL/J

• immune system phenotype
• brain inflammation (MGI Ref ID J:92227)
  • severely increased meningeal inflammation on both PL/J and SJL/J backgrounds after infection with TMEV
• increased susceptibility to viral infection (MGI Ref ID J:92227)
  • inflammation persists in the susceptible SJL/J background, particularly in the cerebellum, brainstem and striatum
• nervous system phenotype
• brain inflammation (MGI Ref ID J:92227)
  • severely increased meningeal inflammation on both PL/J and SJL/J backgrounds after infection with TMEV
• demyelination (MGI Ref ID J:92227)
  • demyelination was severe on both the susceptible PL/J and SJL/J backgrounds after infection with TMEV

Cd4^tm1Mak/Cd4^tm1Mak

        involves: 129S2/SvPas

• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:110749)
  • mice clear polyomavirus within one month of infection similar to controls

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Immunology and Inflammation Research
Immunodeficiency (T cell deficiency)

Research Tools
Immunology and Inflammation Research (genes regulating susceptibility to infectious disease and endotoxin)

Virology Research

Cd4^tm1Mak related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cd4tm1Mak
Allele Name		targeted mutation 1, Tak Mak
Allele Type		Targeted (knock-out)
Common Name(s)		CD4 KO; CD4-; CD40;
Mutation Made By		Tak Mak,   University Health Network/Un of Toronto
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Cd4, CD4 antigen
Chromosome		6
Gene Common Name(s)		CD4mut; L3T4; Ly-4; W3/25; lymphocyte antigen 4; p55;
Molecular Note		A neomycin resistance cassette was inserted into exon 5. Flow cytometry analysis demonstrated that the protein was absent from the cell surface of thymocytes and lymph node cells in homozygous mice. [MGI Ref ID J:68957]

Genotyping

Genotyping Information

Genotyping Protocols

Generic Cd4, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Rahemtulla A; Fung-Leung WP; Schilham MW; Kundig TM; Sambhara SR; Narendran A; Arabian A; Wakeham A; Paige CJ; Zinkernagel RM; et al.. 1991. Normal development and function of CD8+ cells but markedly decreased helper cell activity in mice lacking CD4. Nature 353(6340):180-4. [PubMed: 1832488]  [MGI Ref ID J:68957]

Additional References

Battegay M; Moskophidis D; Rahemtulla A; Hengartner H; Mak TW; Zinkernagel RM. 1994. Enhanced establishment of a virus carrier state in adult CD4+ T-cell-deficient mice. J Virol 68(7):4700-4. [PubMed: 7911534]  [MGI Ref ID J:18756]

Bitsaktsis C; Huntington J; Winslow G. 2004. Production of IFN-gamma by CD4 T cells is essential for resolving ehrlichia infection. J Immunol 172(11):6894-901. [PubMed: 15153508]  [MGI Ref ID J:90518]

Chiang EY; Stroynowski I. 2004. A nonclassical MHC class I molecule restricts CTL-mediated rejection of a syngeneic melanoma tumor. J Immunol 173(7):4394-401. [PubMed: 15383569]  [MGI Ref ID J:93729]

Chung DR; Kasper DL; Panzo RJ; Chtinis T; Grusby MJ; Sayegh MH; Tzianabos AO. 2003. CD4+ T cells mediate abscess formation in intra-abdominal sepsis by an IL-17-dependent mechanism. J Immunol 170(4):1958-63. [PubMed: 12574364]  [MGI Ref ID J:81809]

Fischbein MP; Yun J; Laks H; Irie Y; Fishbein MC; Espejo M; Bonavida B; Ardehali A. 2001. CD8+ lymphocytes augment chronic rejection in a MHC class II mismatched model. Transplantation 71(8):1146-53. [PubMed: 11374417]  [MGI Ref ID J:69459]

Fuller MJ; Khanolkar A; Tebo AE; Zajac AJ. 2004. Maintenance, loss, and resurgence of T cell responses during acute, protracted, and chronic viral infections. J Immunol 172(7):4204-14. [PubMed: 15034033]  [MGI Ref ID J:88714]

Kawakami K; Kinjo Y; Yara S; Koguchi Y; Uezu K; Nakayama T; Taniguchi M; Saito A. 2001. Activation of Valpha14(+) natural killer T cells by alpha-galactosylceramide results in development of Th1 response and local host resistance in mice infected with Cryptococcus neoformans. Infect Immun 69(1):213-20. [PubMed: 11119508]  [MGI Ref ID J:67758]

Khanolkar A; Fuller MJ; Zajac AJ. 2004. CD4 T cell-dependent CD8 T cell maturation. J Immunol 172(5):2834-44. [PubMed: 14978084]  [MGI Ref ID J:88234]

Kondo S; Beissert S; Wang B; Fujisawa H; Kooshesh F; Stratigos A; Granstein RD; Mak TW; Sauder DN. 1996. Hyporesponsiveness in contact hypersensitivity and irritant contact dermatitis in CD4 gene targeted mouse. J Invest Dermatol 106(5):993-1000. [PubMed: 8618064]  [MGI Ref ID J:32787]

Lewicki H; Tishon A; Homann D; Mazarguil H; Laval F; Asensio VC; Campbell IL; DeArmond S; Coon B; Teng C; Gairin JE; Oldstone MB. 2003. T cells infiltrate the brain in murine and human transmissible spongiform encephalopathies. J Virol 77(6):3799-808. [PubMed: 12610154]  [MGI Ref ID J:82227]

Mak TW; Rahemtulla A; Schilham M; Koh DR; Fung-Leung WP. 1992. Generation of mutant mice lacking surface expression of CD4 or CD8 by gene targeting. J Autoimmun 5 Suppl A:55-9. [PubMed: 1503636]  [MGI Ref ID J:575]

Mohammed KA; Nasreen N; Ward MJ; Antony VB. 2000. Induction of acute pleural inflammation by Staphylococcus aureus. I. CD4+ T cells play a critical role in experimental empyema. J Infect Dis 181(5):1693-9. [PubMed: 10823770]  [MGI Ref ID J:62683]

Niethammer AG; Xiang R; Ruehlmann JM; Lode HN; Dolman CS; Gillies SD; Reisfeld RA. 2001. Targeted interleukin 2 therapy enhances protective immunity induced by an autologous oral DNA vaccine against murine melanoma. Cancer Res 61(16):6178-84. [PubMed: 11507070]  [MGI Ref ID J:71198]

Pien GC; Nguyen KB; Malmgaard L; Satoskar AR; Biron CA. 2002. A unique mechanism for innate cytokine promotion of T cell responses to viral infections. J Immunol 169(10):5827-37. [PubMed: 12421964]  [MGI Ref ID J:80075]

Serbina NV; Lazarevic V; Flynn JL. 2001. CD4(+) T cells are required for the development of cytotoxic CD8(+) T cells during Mycobacterium tuberculosis infection. J Immunol 167(12):6991-7000. [PubMed: 11739519]  [MGI Ref ID J:73094]

Sha Z; Compans RW. 2000. Induction of CD4(+) T-cell-independent immunoglobulin responses by inactivated influenza virus. J Virol 74(11):4999-5005. [PubMed: 10799573]  [MGI Ref ID J:62131]

Sun JC; Bevan MJ. 2004. Cutting edge: long-lived CD8 memory and protective immunity in the absence of CD40 expression on CD8 T cells. J Immunol 172(6):3385-9. [PubMed: 15004136]  [MGI Ref ID J:88609]

Tyznik AJ; Sun JC; Bevan MJ. 2004. The CD8 population in CD4-deficient mice is heavily contaminated with MHC class II-restricted T cells. J Exp Med 199(4):559-65. [PubMed: 14769854]  [MGI Ref ID J:90469]

VanCott JL; McNeal MM; Flint J; Bailey SA; Choi AH; Ward RL. 2001. Role for T cell-independent B cell activity in the resolution of primary rotavirus infection in mice. Eur J Immunol 31(11):3380-7. [PubMed: 11745356]  [MGI Ref ID J:72616]

Cd4^tm1Mak related

Adoro S; Erman B; Sarafova SD; Van Laethem F; Park JH; Feigenbaum L; Singer A. 2008. Targeting CD4 coreceptor expression to postselection thymocytes reveals that CD4/CD8 lineage choice is neither error-prone nor stochastic. J Immunol 181(10):6975-83. [PubMed: 18981117]  [MGI Ref ID J:140942]

Antony PA; Piccirillo CA; Akpinarli A; Finkelstein SE; Speiss PJ; Surman DR; Palmer DC; Chan CC; Klebanoff CA; Overwijk WW; Rosenberg SA; Restifo NP. 2005. CD8+ T cell immunity against a tumor/self-antigen is augmented by CD4+ T helper cells and hindered by naturally occurring T regulatory cells. J Immunol 174(5):2591-601. [PubMed: 15728465]  [MGI Ref ID J:129825]

Bachmaier K; Neu N; Yeung RS; Mak TW; Liu P; Penninger JM. 1999. Generation of humanized mice susceptible to peptide-induced inflammatory heart disease. Circulation 99(14):1885-91. [PubMed: 10199887]  [MGI Ref ID J:129115]

Battegay M; Moskophidis D; Rahemtulla A; Hengartner H; Mak TW; Zinkernagel RM. 1994. Enhanced establishment of a virus carrier state in adult CD4+ T-cell-deficient mice. J Virol 68(7):4700-4. [PubMed: 7911534]  [MGI Ref ID J:18756]

Berke Z; Wen T; Jin S; Klein G; Dalianis T. 1995. Polyomavirus persists in CD4/8 double-knockout, but not in CD4 or CD8 single-knockout mice. Virology 212(1):268-71. [PubMed: 7676644]  [MGI Ref ID J:110749]

Berke Z; Wen T; Klein G; Dalianis T. 1996. Polyoma tumor development in neonatally polyoma-virus-infected CD4-/- and CD8-/- single knockout and CD4-/-8-/- double knockout mice. Int J Cancer 67(3):405-8. [PubMed: 8707416]  [MGI Ref ID J:113042]

Bitsaktsis C; Huntington J; Winslow G. 2004. Production of IFN-gamma by CD4 T cells is essential for resolving ehrlichia infection. J Immunol 172(11):6894-901. [PubMed: 15153508]  [MGI Ref ID J:90518]

Bitsaktsis C; Nandi B; Racine R; MacNamara KC; Winslow G. 2007. T-Cell-independent humoral immunity is sufficient for protection against fatal intracellular ehrlichia infection. Infect Immun 75(10):4933-41. [PubMed: 17664264]  [MGI Ref ID J:125283]

Black KE; Murray JA; David CS. 2002. HLA-DQ Determines the Response to Exogenous Wheat Proteins: A Model of Gluten Sensitivity in Transgenic Knockout Mice. J Immunol 169(10):5595-600. [PubMed: 12421937]  [MGI Ref ID J:80059]

Blazar BR; Lees CJ; Martin PJ; Noelle RJ; Kwon B; Murphy W; Taylor PA. 2000. Host T cells resist graft-versus-host disease mediated by donor leukocyte infusions. J Immunol 165(9):4901-9. [PubMed: 11046015]  [MGI Ref ID J:118027]

Borenstein SH; Graham J; Zhang XL; Chamberlain JW. 2000. CD8+ T cells are necessary for recognition of allelic, but not locus-mismatched or xeno-, HLA class I transplantation antigens. J Immunol 165(5):2341-53. [PubMed: 10946256]  [MGI Ref ID J:80678]

Burne MJ; Daniels F; El Ghandour A; Mauiyyedi S; Colvin RB; O'Donnell MP; Rabb H. 2001. Identification of the CD4(+) T cell as a major pathogenic factor in ischemic acute renal failure. J Clin Invest 108(9):1283-90. [PubMed: 11696572]  [MGI Ref ID J:118005]

Caldwell CC; Okaya T; Martignoni A; Husted T; Schuster R; Lentsch AB. 2005. Divergent functions of CD4+ T lymphocytes in acute liver inflammation and injury after ischemia-reperfusion. Am J Physiol Gastrointest Liver Physiol 289(5):G969-76. [PubMed: 16002566]  [MGI Ref ID J:104787]

Chan K; Lee DJ; Schubert A; Tang CM; Crain B; Schoenberger SP; Corr M. 2001. The roles of MHC class II, CD40, and B7 costimulation in CTL induction by plasmid DNA. J Immunol 166(5):3061-6. [PubMed: 11207256]  [MGI Ref ID J:126695]

Chan WC; Duong TT; Yeung RS. 2004. Presence of IFN-gamma does not indicate its necessity for induction of coronary arteritis in an animal model of Kawasaki disease. J Immunol 173(5):3492-503. [PubMed: 15322214]  [MGI Ref ID J:92708]

Chapoval SP; Iijima K; Marietta EV; Smart MK; Chapoval AI; Andrews AG; David CS. 2002. Allergic inflammatory response to short ragweed allergenic extract in HLA-DQ transgenic mice lacking CD4 gene. J Immunol 168(2):890-9. [PubMed: 11777987]  [MGI Ref ID J:73744]

Chapoval SP; Marietta EV; Smart MK; David CS. 2001. Requirements for allergen-induced airway inflammation and hyperreactivity in CD4-deficient and CD4-sufficient HLA-DQ transgenic mice. J Allergy Clin Immunol 108(5):764-71. [PubMed: 11692102]  [MGI Ref ID J:106479]

Chen SY; Takeoka Y; Ansari AA; Boyd R; Klinman DM; Gershwin ME. 1996. The natural history of disease expression in CD4 and CD8 gene-deleted New Zealand black (NZB) mice. J Immunol 157(6):2676-84. [PubMed: 8805673]  [MGI Ref ID J:35433]

Chen Z; Yu S; Concha HQ; Zhu Y; Mix E; Winblad B; Ljunggren HG; Zhu J. 2004. Kainic acid-induced excitotoxic hippocampal neurodegeneration in C57BL/6 mice: B cell and T cell subsets may contribute differently to the pathogenesis. Brain Behav Immun 18(2):175-85. [PubMed: 14759595]  [MGI Ref ID J:105331]

Chiang EY; Stroynowski I. 2004. A nonclassical MHC class I molecule restricts CTL-mediated rejection of a syngeneic melanoma tumor. J Immunol 173(7):4394-401. [PubMed: 15383569]  [MGI Ref ID J:93729]

Choudhury A; Maldonado MA; Cohen PL; Eisenberg RA. 2005. The role of host CD4 T cells in the pathogenesis of the chronic graft-versus-host model of systemic lupus erythematosus. J Immunol 174(12):7600-9. [PubMed: 15944260]  [MGI Ref ID J:100787]

Daniel D; Meyer-Morse N; Bergsland EK; Dehne K; Coussens LM; Hanahan D. 2003. Immune Enhancement of Skin Carcinogenesis by CD4+ T Cells. J Exp Med 197(8):1017-28. [PubMed: 12695493]  [MGI Ref ID J:82978]

Dautigny N; Le Campion A; Lucas B. 1999. Timing and casting for actors of thymic negative selection. J Immunol 162(3):1294-302. [PubMed: 9973382]  [MGI Ref ID J:124433]

Derrick SC; Evering TH; Sambandamurthy VK; Jalapathy KV; Hsu T; Chen B; Chen M; Russell RG; Junqueira-Kipnis AP; Orme IM; Porcelli SA; Jacobs WR Jr; Morris SL. 2007. Characterization of the protective T-cell response generated in CD4-deficient mice by a live attenuated Mycobacterium tuberculosis vaccine. Immunology 120(2):192-206. [PubMed: 17076705]  [MGI Ref ID J:122314]

Duong TT; Silverman ED; Bissessar MV; Yeung RS. 2003. Superantigenic activity is responsible for induction of coronary arteritis in mice: an animal model of Kawasaki disease. Int Immunol 15(1):79-89. [PubMed: 12502728]  [MGI Ref ID J:81746]

Ehinger M; Vestberg M; Johansson AC; Johannesson M; Svensson A; Holmdahl R. 2001. Influence of CD4 or CD8 deficiency on collagen-induced arthritis. Immunology 103(3):291-300. [PubMed: 11454058]  [MGI Ref ID J:110421]

Fischbein MP; Ardehali A; Yun J; Schoenberger S; Laks H; Irie Y; Dempsey P; Cheng G; Fishbein MC; Bonavida B. 2000. CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants. J Immunol 165(12):7316-22. [PubMed: 11120867]  [MGI Ref ID J:118395]

Flano E; Woodland DL; Blackman MA. 1999. Requirement for CD4+ T cells in V beta 4+CD8+ T cell activation associated with latent murine gammaherpesvirus infection. J Immunol 163(6):3403-8. [PubMed: 10477611]  [MGI Ref ID J:119171]

Freland S; Ljunggren H. 2000. beta2-Microglobulin/CD8 -/- mice reveal significant role for CD8+ T cells in graft rejection responses in beta2-microglobulin -/- mice Scand J Immunol 51(3):219-23. [PubMed: 10736089]  [MGI Ref ID J:61231]

Fuller MJ; Khanolkar A; Tebo AE; Zajac AJ. 2004. Maintenance, loss, and resurgence of T cell responses during acute, protracted, and chronic viral infections. J Immunol 172(7):4204-14. [PubMed: 15034033]  [MGI Ref ID J:88714]

Grayson MH; Cheung D; Rohlfing MM; Kitchens R; Spiegel DE; Tucker J; Battaile JT; Alevy Y; Yan L; Agapov E; Kim EY; Holtzman MJ. 2007. Induction of high-affinity IgE receptor on lung dendritic cells during viral infection leads to mucous cell metaplasia. J Exp Med 204(11):2759-69. [PubMed: 17954569]  [MGI Ref ID J:126124]

Grueter B; Petter M; Egawa T; Laule-Kilian K; Aldrian CJ; Wuerch A; Ludwig Y; Fukuyama H; Wardemann H; Waldschuetz R; Moroy T; Taniuchi I; Steimle V; Littman DR; Ehlers M. 2005. Runx3 regulates integrin alpha E/CD103 and CD4 expression during development of CD4-/CD8+ T cells. J Immunol 175(3):1694-705. [PubMed: 16034110]  [MGI Ref ID J:107280]

Heemskerk MH; Schilham MW; Schoemaker HM; Spierenburg G; Spaan WJ; Boog CJ. 1995. Activation of virus-specific major histocompatibility complex class II-restricted CD8+ cytotoxic T cells in CD4-deficient mice. Eur J Immunol 25(4):1109-12. [PubMed: 7737281]  [MGI Ref ID J:112983]

Hida S; Ogasawara K; Sato K; Abe M; Takayanagi H; Yokochi T; Sato T; Hirose S; Shirai T; Taki S; Taniguchi T. 2000. CD8(+) T cell-mediated skin disease in mice lacking IRF-2, the transcriptional attenuator of interferon-alpha/beta signaling Immunity 13(5):643-55. [PubMed: 11114377]  [MGI Ref ID J:66034]

Hornquist CE; Ekman L; Grdic KD; Schon K; Lycke NY. 1995. Paradoxical IgA immunity in CD4-deficient mice. Lack of cholera toxin-specific protective immunity despite normal gut mucosal IgA differentiation. J Immunol 155(6):2877-87. [PubMed: 7673704]  [MGI Ref ID J:110845]

Howe CL; Adelson JD; Rodriguez M. 2007. Absence of perforin expression confers axonal protection despite demyelination. Neurobiol Dis 25(2):354-9. [PubMed: 17112732]  [MGI Ref ID J:119009]

Huber SA; Sakkinen P; David C; Newell MK; Tracy RP. 2001. T helper-cell phenotype regulates atherosclerosis in mice under conditions of mild hypercholesterolemia. Circulation 103(21):2610-6. [PubMed: 11382732]  [MGI Ref ID J:133189]

Hung K; Hayashi R; Lafond-Walker A; Lowenstein C; Pardoll D; Levitsky H. 1998. The central role of CD4(+) T cells in the antitumor immune response. J Exp Med 188(12):2357-68. [PubMed: 9858522]  [MGI Ref ID J:51677]

Intlekofer AM; Takemoto N; Kao C; Banerjee A; Schambach F; Northrop JK; Shen H; Wherry EJ; Reiner SL. 2007. Requirement for T-bet in the aberrant differentiation of unhelped memory CD8+ T cells. J Exp Med 204(9):2015-21. [PubMed: 17698591]  [MGI Ref ID J:126087]

Ip CW; Kroner A; Bendszus M; Leder C; Kobsar I; Fischer S; Wiendl H; Nave KA; Martini R. 2006. Immune cells contribute to myelin degeneration and axonopathic changes in mice overexpressing proteolipid protein in oligodendrocytes. J Neurosci 26(31):8206-16. [PubMed: 16885234]  [MGI Ref ID J:111136]

Joetham A; Takeda K; Taube C; Miyahara N; Kanehiro A; Dakhama A; Gelfand EW. 2005. Airway hyperresponsiveness in the absence of CD4+ T cells after primary but not secondary challenge. Am J Respir Cell Mol Biol 33(1):89-96. [PubMed: 15845865]  [MGI Ref ID J:110969]

Kamperschroer C; Roberts DM; Zhang Y; Weng NP; Swain SL. 2008. SAP enables T cells to help B cells by a mechanism distinct from Th cell programming or CD40 ligand regulation. J Immunol 181(6):3994-4003. [PubMed: 18768854]  [MGI Ref ID J:139100]

Khan IA; Schwartzman JD; Kasper LH; Moretto M. 1999. CD8+ CTLs are essential for protective immunity against Encephalitozoon cuniculi infection. J Immunol 162(10):6086-91. [PubMed: 10229850]  [MGI Ref ID J:120238]

Khanolkar A; Fuller MJ; Zajac AJ. 2004. CD4 T cell-dependent CD8 T cell maturation. J Immunol 172(5):2834-44. [PubMed: 14978084]  [MGI Ref ID J:88234]

Khiong K; Murakami M; Kitabayashi C; Ueda N; Sawa S; Sakamoto A; Kotzin BL; Rozzo SJ; Ishihara K; Verella-Garcia M; Kappler J; Marrack P; Hirano T. 2007. Homeostatically proliferating CD4 T cells are involved in the pathogenesis of an Omenn syndrome murine model. J Clin Invest 117(5):1270-81. [PubMed: 17476359]  [MGI Ref ID J:122142]

Kikuchi T; Andarini S; Xin H; Gomi K; Tokue Y; Saijo Y; Honjo T; Watanabe A; Nukiwa T. 2005. Involvement of fractalkine/CX3CL1 expression by dendritic cells in the enhancement of host immunity against Legionella pneumophila. Infect Immun 73(9):5350-7. [PubMed: 16113250]  [MGI Ref ID J:100418]

Kim DK; Tahara-Hanaoka S; Shinohara N; Nakauchi H. 2007. A human mutant CD4 molecule resistant to HIV-1 binding restores helper T-lymphocyte functions in murine CD4-deficient mice. Exp Mol Med 39(1):1-7. [PubMed: 17334223]  [MGI Ref ID J:134164]

Klein MA; Frigg R; Flechsig E; Raeber AJ; Kalinke U; Bluethmann H; Bootz F; Suter M; Zinkernagel RM; Aguzzi A. 1997. A crucial role for B cells in neuroinvasive scrapie [see comments] Nature 390(6661):687-90. [PubMed: 9414161]  [MGI Ref ID J:44933]

Koh DR; Ho A; Rahemtulla A; Fung-Leung WP; Griesser H; Mak TW. 1995. Murine lupus in MRL/lpr mice lacking CD4 or CD8 T cells. Eur J Immunol 25(9):2558-62. [PubMed: 7589126]  [MGI Ref ID J:28923]

Koh DR; Ho A; Rahemtulla A; Penninger J; Mak TW. 1994. Experimental allergic encephalomyelitis (EAE) in mice lacking CD4+ T cells. Eur J Immunol 24(9):2250-3. [PubMed: 7916298]  [MGI Ref ID J:112987]

Kohu K; Sato T; Ohno S; Hayashi K; Uchino R; Abe N; Nakazato M; Yoshida N; Kikuchi T; Iwakura Y; Inoue Y; Watanabe T; Habu S; Satake M. 2005. Overexpression of the Runx3 transcription factor increases the proportion of mature thymocytes of the CD8 single-positive lineage. J Immunol 174(5):2627-36. [PubMed: 15728469]  [MGI Ref ID J:97715]

Kondo S; Beissert S; Wang B; Fujisawa H; Kooshesh F; Stratigos A; Granstein RD; Mak TW; Sauder DN. 1996. Hyporesponsiveness in contact hypersensitivity and irritant contact dermatitis in CD4 gene targeted mouse. J Invest Dermatol 106(5):993-1000. [PubMed: 8618064]  [MGI Ref ID J:32787]

Kontoyiannis D; Boulougouris G; Manoloukos M; Armaka M; Apostolaki M; Pizarro T; Kotlyarov A; Forster I; Flavell R; Gaestel M; Tsichlis P; Cominelli F; Kollias G. 2002. Genetic dissection of the cellular pathways and signaling mechanisms in modeled tumor necrosis factor-induced Crohn's-like inflammatory bowel disease. J Exp Med 196(12):1563-74. [PubMed: 12486099]  [MGI Ref ID J:108572]

Law YM; Yeung RS; Mamalaki C; Kioussis D; Mak TW; Flavell RA. 1994. Human CD4 restores normal T cell development and function in mice deficient in murine CD4. J Exp Med 179(4):1233-42. [PubMed: 8145040]  [MGI Ref ID J:77270]

Lepisto AJ; Frank GM; Xu M; Stuart PM; Hendricks RL. 2006. CD8 T cells mediate transient herpes stromal keratitis in CD4-deficient mice. Invest Ophthalmol Vis Sci 47(8):3400-9. [PubMed: 16877409]  [MGI Ref ID J:112225]

Lesage S; Steff AM; Philippoussis F; Page M; Trop S; Mateo V ; Hugo P. 1997. CD4+ CD8+ thymocytes are preferentially induced to die following CD45 cross-linking, through a novel apoptotic pathway. J Immunol 159(10):4762-71. [PubMed: 9366400]  [MGI Ref ID J:44075]

Lin X; Ma X; Rodriguez M; Roos RP. 2004. CD4+ T cells are important for clearance of DA strain of TMEV from the central nervous system of SJL/J mice. Int Immunol 16(9):1237-40. [PubMed: 15262897]  [MGI Ref ID J:91962]

Lin X; Pease LR; Murray PD; Rodriguez M. 1998. Theiler's virus infection of genetically susceptible mice induces central nervous system-infiltrating CTLs with no apparent viral or major myelin antigenic specificity. J Immunol 160(11):5661-8. [PubMed: 9605173]  [MGI Ref ID J:47789]

Liu M; Chien CC; Burne-Taney M; Molls RR; Racusen LC; Colvin RB; Rabb H. 2006. A pathophysiologic role for T lymphocytes in murine acute cisplatin nephrotoxicity. J Am Soc Nephrol 17(3):765-74. [PubMed: 16481417]  [MGI Ref ID J:135790]

Liu P; Aitken K; Kong YY; Opavsky MA; Martino T; Dawood F; Wen WH; Kozieradzki I; Bachmaier K; Straus D; Mak TW; Penninger JM. 2000. The tyrosine kinase p56lck is essential in coxsackievirus B3-mediated heart disease. Nat Med 6(4):429-34. [PubMed: 10742150]  [MGI Ref ID J:119597]

Lucas PJ; Kim SJ; Mackall CL; Telford WG; Chu YW; Hakim FT; Gress RE. 2006. Dysregulation of IL-15-mediated T-cell homeostasis in TGF-beta dominant-negative receptor transgenic mice. Blood 108(8):2789-95. [PubMed: 16788095]  [MGI Ref ID J:139469]

Lunsford KE; Koester MA; Eiring AM; Horne PH; Gao D; Bumgardner GL. 2005. Targeting LFA-1 and cd154 suppresses the in vivo activation and development of cytolytic (cd4-Independent) CD8+ T cells. J Immunol 175(12):7855-66. [PubMed: 16339521]  [MGI Ref ID J:122220]

Lynch EA; Heijens CA; Horst NF; Center DM; Cruikshank WW. 2003. Cutting edge: IL-16/CD4 preferentially induces Th1 cell migration: requirement of CCR5. J Immunol 171(10):4965-8. [PubMed: 14607889]  [MGI Ref ID J:135472]

Mak TW; Rahemtulla A; Schilham M; Koh DR; Fung-Leung WP. 1992. Generation of mutant mice lacking surface expression of CD4 or CD8 by gene targeting. J Autoimmun 5 Suppl A:55-9. [PubMed: 1503636]  [MGI Ref ID J:575]

Matheson JM; Johnson VJ; Luster MI. 2005. Immune mediators in a murine model for occupational asthma: studies with toluene diisocyanate. Toxicol Sci 84(1):99-109. [PubMed: 15590890]  [MGI Ref ID J:106452]

McCausland MM; Yusuf I; Tran H; Ono N; Yanagi Y; Crotty S. 2007. SAP regulation of follicular helper CD4 T cell development and humoral immunity is independent of SLAM and Fyn kinase. J Immunol 178(2):817-28. [PubMed: 17202343]  [MGI Ref ID J:133047]

McCoy KD; Harris NL; Diener P; Hatak S; Odermatt B; Hangartner L; Senn BM; Marsland BJ; Geuking MB; Hengartner H; Macpherson AJ; Zinkernagel RM. 2006. Natural IgE production in the absence of MHC Class II cognate help. Immunity 24(3):329-39. [PubMed: 16546101]  [MGI Ref ID J:113324]

Mishra A; Schlotman J; Wang M; Rothenberg ME. 2007. Critical role for adaptive T cell immunity in experimental eosinophilic esophagitis in mice. J Leukoc Biol 81(4):916-24. [PubMed: 17194734]  [MGI Ref ID J:121455]

Murray PD; Pavelko KD; Leibowitz J; Lin X; Rodriguez M. 1998. CD4(+) and CD8(+) T cells make discrete contributions to demyelination and neurologic disease in a viral model of multiple sclerosis. J Virol 72(9):7320-9. [PubMed: 9696828]  [MGI Ref ID J:92227]

Newell KA; He G; Guo Z; Kim O; Szot GL; Rulifson I; Zhou P; Hart J; Thistlethwaite JR; Bluestone JA. 1999. Cutting edge: blockade of the CD28/B7 costimulatory pathway inhibits intestinal allograft rejection mediated by CD4+ but not CD8+ T cells. J Immunol 163(5):2358-62. [PubMed: 10452966]  [MGI Ref ID J:57103]

Ngai P; McCormick S; Small C; Zhang X; Zganiacz A; Aoki N; Xing Z. 2007. Gamma interferon responses of CD4 and CD8 T-cell subsets are quantitatively different and independent of each other during pulmonary Mycobacterium bovis BCG infection. Infect Immun 75(5):2244-52. [PubMed: 17307945]  [MGI Ref ID J:121875]

Northrop JK; Thomas RM; Wells AD; Shen H. 2006. Epigenetic remodeling of the IL-2 and IFN-gamma loci in memory CD8 T cells is influenced by CD4 T cells. J Immunol 177(2):1062-9. [PubMed: 16818762]  [MGI Ref ID J:134945]

Olasz EB; Roh J; Yee CL; Arita K; Akiyama M; Shimizu H; Vogel JC; Yancey KB. 2007. Human bullous pemphigoid antigen 2 transgenic skin elicits specific IgG in wild-type mice. J Invest Dermatol 127(12):2807-17. [PubMed: 17657247]  [MGI Ref ID J:127328]

Oliveira-dos-Santos AJ; Penninger JM; Rieker-Geley T; Matsumoto G; Mak TM; Wick G. 1998. Thymic heterotypic cellular complexes in gene-targeted mice with defined blocks in T cell development and adhesion molecule expression. Eur J Immunol 28(9):2882-92. [PubMed: 9754575]  [MGI Ref ID J:49877]

Opavsky MA; Penninger J; Aitken K; Wen WH; Dawood F; Mak T; Liu P. 1999. Susceptibility to myocarditis is dependent on the response of alphabeta T lymphocytes to coxsackieviral infection. Circ Res 85(6):551-8. [PubMed: 10488058]  [MGI Ref ID J:114217]

Papouchado BG; Chapoval SP; Marietta EV; Weiler CR; David CS. 2001. Cockroach allergen-induced eosinophilic airway inflammation in HLA-DQ/human CD4(+) transgenic mice. J Immunol 167(8):4627-34. [PubMed: 11591792]  [MGI Ref ID J:130539]

Park JH; Adoro S; Lucas PJ; Sarafova SD; Alag AS; Doan LL; Erman B; Liu X; Ellmeier W; Bosselut R; Feigenbaum L; Singer A. 2007. 'Coreceptor tuning': cytokine signals transcriptionally tailor CD8 coreceptor expression to the self-specificity of the TCR. Nat Immunol 8(10):1049-59. [PubMed: 17873878]  [MGI Ref ID J:125259]

Pearce EL; Shedlock DJ; Shen H. 2004. Functional characterization of MHC class II-restricted CD8+CD4- and CD8-CD4- T cell responses to infection in CD4-/- mice. J Immunol 173(4):2494-9. [PubMed: 15294964]  [MGI Ref ID J:92690]

Penninger JM; Neu N; Timms E; Wallace VA; Koh DR; Kishihara K; Pummerer C; Mak TW. 1993. The induction of experimental autoimmune myocarditis in mice lacking CD4 or CD8 molecules [corrected] [published erratum appears in J Exp Med 1994 Jan 1;179(1):371] J Exp Med 178(5):1837-42. [PubMed: 8228830]  [MGI Ref ID J:15279]

Penninger JM; Sirard C; Mittrucker HW; Chidgey A; Kozieradzki I; Nghiem M; Hakem A; Kimura T; Timms E; Boyd R; Taniguchi T; Matsuyama T; Mak TW. 1997. The interferon regulatory transcription factor IRF-1 controls positive and negative selection of CD8+ thymocytes. Immunity 7(2):243-54. [PubMed: 9285409]  [MGI Ref ID J:111439]

Pien GC; Nguyen KB; Malmgaard L; Satoskar AR; Biron CA. 2002. A unique mechanism for innate cytokine promotion of T cell responses to viral infections. J Immunol 169(10):5827-37. [PubMed: 12421964]  [MGI Ref ID J:80075]

Rabb H; Daniels F; O'Donnell M; Haq M; Saba SR; Keane W; Tang WW. 2000. Pathophysiological role of T lymphocytes in renal ischemia-reperfusion injury in mice. Am J Physiol Renal Physiol 279(3):F525-31. [PubMed: 10966932]  [MGI Ref ID J:114177]

Rahemtulla A; Kundig TM; Narendran A; Bachmann MF; Julius M; Paige CJ; Ohashi PS; Zinkernagel RM; Mak TW. 1994. Class II major histocompatibility complex-restricted T cell function in CD4-deficient mice. Eur J Immunol 24(9):2213-8. [PubMed: 7916297]  [MGI Ref ID J:112988]

Rahemtulla A; Shahinian A; Kundig T; Zinkernagel R; Mak TW. 1993. CD4 negative mice as a model for immunodeficiency. Philos Trans R Soc Lond B Biol Sci 342(1299):57-8. [PubMed: 7904347]  [MGI Ref ID J:20507]

Roberts SJ; Ng BY; Filler RB; Lewis J; Glusac EJ; Hayday AC; Tigelaar RE; Girardi M. 2007. Characterizing tumor-promoting T cells in chemically induced cutaneous carcinogenesis. Proc Natl Acad Sci U S A 104(16):6770-5. [PubMed: 17412837]  [MGI Ref ID J:120942]

Rolan HG; Tsolis RM. 2007. Mice lacking components of adaptive immunity show increased Brucella abortus virB mutant colonization. Infect Immun 75(6):2965-73. [PubMed: 17420243]  [MGI Ref ID J:121919]

Rottenberg ME; Bakhiet M; Olsson T; Kristensson K; Mak T; Wigzell H; Orn A. 1993. Differential susceptibilities of mice genomically deleted of CD4 and CD8 to infections with Trypanosoma cruzi or Trypanosoma brucei. Infect Immun 61(12):5129-33. [PubMed: 8225589]  [MGI Ref ID J:111017]

Rottenberg ME; Gigliotti Rothfuchs AC; Gigliotti D; Svanholm C; Bandholtz L; Wigzell H. 1999. Role of innate and adaptive immunity in the outcome of primary infection with Chlamydia pneumoniae, as analyzed in genetically modified mice. J Immunol 162(5):2829-36. [PubMed: 10072530]  [MGI Ref ID J:124536]

Rottenberg ME; Riarte A; Sporrong L; Altcheh J; Petray P; Ruiz AM; Wigzell H; Orn A. 1995. Outcome of infection with different strains of Trypanosoma cruzi in mice lacking CD4 and/or CD8. Immunol Lett 45(1-2):53-60. [PubMed: 7622189]  [MGI Ref ID J:112349]

Sarafova SD; Erman B; Yu Q; Van Laethem F; Guinter T; Sharrow SO; Feigenbaum L; Wildt KF; Ellmeier W; Singer A. 2005. Modulation of coreceptor transcription during positive selection dictates lineage fate independently of TCR/coreceptor specificity. Immunity 23(1):75-87. [PubMed: 16039581]  [MGI Ref ID J:100541]

Sardinha LR; Elias RM; Mosca T; Bastos KR; Marinho CR; D'Imperio Lima MR; Alvarez JM. 2006. Contribution of NK, NK T, gammadelta T, and alphabeta T cells to the gamma interferon response required for liver protection against Trypanosoma cruzi. Infect Immun 74(4):2031-42. [PubMed: 16552032]  [MGI Ref ID J:107423]

Sawa S; Kamimura D; Jin GH; Morikawa H; Kamon H; Nishihara M; Ishihara K; Murakami M; Hirano T. 2006. Autoimmune arthritis associated with mutated interleukin (IL)-6 receptor gp130 is driven by STAT3/IL-7-dependent homeostatic proliferation of CD4+ T cells. J Exp Med 203(6):1459-70. [PubMed: 16717113]  [MGI Ref ID J:124381]

Schilham MW; Fung-Leung WP; Rahemtulla A; Kuendig T; Zhang L; Potter J; Miller RG; Hengartner H; Mak TW. 1993. Alloreactive cytotoxic T cells can develop and function in mice lacking both CD4 and CD8. Eur J Immunol 23(6):1299-304. [PubMed: 8500525]  [MGI Ref ID J:12572]

Senaldi G; Shaklee CL; Mak TW; Ulich TR. 1999. Corynebacterium parvum- and Mycobacterium bovis Bacillus Calmette and Guerin-induced granuloma formation in mice lacking CD4 and CD8. Cell Immunol 193(2):155-61. [PubMed: 10222057]  [MGI Ref ID J:114269]

Serbina NV; Lazarevic V; Flynn JL. 2001. CD4(+) T cells are required for the development of cytotoxic CD8(+) T cells during Mycobacterium tuberculosis infection. J Immunol 167(12):6991-7000. [PubMed: 11739519]  [MGI Ref ID J:73094]

Shedlock DJ; Whitmire JK; Tan J; MacDonald AS; Ahmed R; Shen H. 2003. Role of CD4 T cell help and costimulation in CD8 T cell responses during Listeria monocytogenes infection. J Immunol 170(4):2053-63. [PubMed: 12574376]  [MGI Ref ID J:126911]

Shen H; Whitmire JK; Fan X; Shedlock DJ; Kaech SM; Ahmed R. 2003. A specific role for B cells in the generation of CD8 T cell memory by recombinant Listeria monocytogenes. J Immunol 170(3):1443-51. [PubMed: 12538706]  [MGI Ref ID J:126273]

Shi FD; Li H; Wang H; Bai X; van der Meide PH; Link H; Ljunggren HG. 1999. Mechanisms of nasal tolerance induction in experimental autoimmune myasthenia gravis: identification of regulatory cells. J Immunol 162(10):5757-63. [PubMed: 10229808]  [MGI Ref ID J:120564]

Shi M; Wei G; Pan W; Tabel H. 2006. Experimental African trypanosomiasis: a subset of pathogenic, IFN-gamma-producing, MHC class II-restricted CD4+ T cells mediates early mortality in highly susceptible mice. J Immunol 176(3):1724-32. [PubMed: 16424202]  [MGI Ref ID J:126643]

Simard C; Klein SJ; Mak T; Jolicoeur P. 1997. Studies of the susceptibility of nude, CD4 knockout, and SCID mutant mice to the disease induced by the murine AIDS defective virus. J Virol 71(4):3013-22. [PubMed: 9060661]  [MGI Ref ID J:38897]

Skelsey ME; Mayhew E; Niederkorn JY. 2003. CD25+, interleukin-10-producing CD4+ T cells are required for suppressor cell production and immune privilege in the anterior chamber of the eye. Immunology 110(1):18-29. [PubMed: 12941137]  [MGI Ref ID J:113598]

Strid J; Roberts SJ; Filler RB; Lewis JM; Kwong BY; Schpero W; Kaplan DH; Hayday AC; Girardi M. 2008. Acute upregulation of an NKG2D ligand promotes rapid reorganization of a local immune compartment with pleiotropic effects on carcinogenesis. Nat Immunol 9(2):146-54. [PubMed: 18176566]  [MGI Ref ID J:131603]

Sun K; Metzger DW. 2008. Inhibition of pulmonary antibacterial defense by interferon-gamma during recovery from influenza infection. Nat Med 14(5):558-64. [PubMed: 18438414]  [MGI Ref ID J:136669]

Sun Y; Blink SE; Chen JH; Fu YX. 2005. Regulation of follicular dendritic cell networks by activated T cells: the role of CD137 signaling. J Immunol 175(2):884-90. [PubMed: 16002686]  [MGI Ref ID J:100693]

Tada Y; Ho A; Koh DR; Mak TW. 1996. Collagen-induced arthritis in CD4- or CD8-deficient mice: CD8+ T cells play a role in initiation and regulate recovery phase of collagen-induced arthritis. J Immunol 156(11):4520-6. [PubMed: 8666829]  [MGI Ref ID J:110669]

Taneja V; Taneja N; Paisansinsup T; Behrens M; Griffiths M; Luthra H; David CS. 2002. CD4 and CD8 T cells in susceptibility/protection to collagen-induced arthritis in HLA-DQ8-transgenic mice: implications for rheumatoid arthritis. J Immunol 168(11):5867-75. [PubMed: 12023391]  [MGI Ref ID J:134936]

Trcka J; Moroi Y; Clynes RA; Goldberg SM; Bergtold A; Perales MA; Ma M; Ferrone CR; Carroll MC; Ravetch JV; Houghton AN. 2002. Redundant and alternative roles for activating Fc receptors and complement in an antibody-dependent model of autoimmune vitiligo. Immunity 16(6):861-8. [PubMed: 12121667]  [MGI Ref ID J:113538]

Tyznik AJ; Sun JC; Bevan MJ. 2004. The CD8 population in CD4-deficient mice is heavily contaminated with MHC class II-restricted T cells. J Exp Med 199(4):559-65. [PubMed: 14769854]  [MGI Ref ID J:90469]

Tzelepis F; Persechini PM; Rodrigues MM. 2007. Modulation of CD4+ T cell-dependent specific cytotoxic CD8+ T cells differentiation and proliferation by the timing of increase in the pathogen load. PLoS ONE 2(4):e393. [PubMed: 17460760]  [MGI Ref ID J:129273]

VanCott JL; McNeal MM; Flint J; Bailey SA; Choi AH; Ward RL. 2001. Role for T cell-independent B cell activity in the resolution of primary rotavirus infection in mice. Eur J Immunol 31(11):3380-7. [PubMed: 11745356]  [MGI Ref ID J:72616]

VanLith ML; Kohlgraf KG; Sivinski CL; Tempero RM; Hollingsworth MA. 2002. MUC1-specific anti-tumor responses: molecular requirements for CD4-mediated responses. Int Immunol 14(8):873-82. [PubMed: 12147624]  [MGI Ref ID J:113544]

Vu MD; Amanullah F; Li Y; Demirci G; Sayegh MH; Li XC. 2004. Different costimulatory and growth factor requirements for CD4+ and CD8+ T cell-mediated rejection. J Immunol 173(1):214-21. [PubMed: 15210777]  [MGI Ref ID J:90930]

Wallace VA; Kondo S; Kono T; Xing Z; Timms E; Furlonger C; Keystone E; Gauldie J; Sauder DN; Mak TW; Paige CJ. 1994. A role for CD4+ T cells in the pathogenesis of skin fibrosis in tight skin mice. Eur J Immunol 24(6):1463-6. [PubMed: 7911425]  [MGI Ref ID J:18913]

Wallace VA; Penninger J; Mak TW. 1994. T-Cell development in CD4, CD8, and p56(lck) Gene-Targeted mice.. In: Transgenesis and Targeted Mutagenesis in Immunology. Academic Press, Inc..  [MGI Ref ID J:21662]

Wallace VA; Rahemtulla A; Timms E; Penninger J; Mak TW. 1992. CD4 expression is differentially required for deletion of MLS-1a-reactive T cells. J Exp Med 176(5):1459-63. [PubMed: 1402689]  [MGI Ref ID J:110753]

Wang B; Fujisawa H; Zhuang L; Freed I; Howell BG; Shahid S; Shivji GM; Mak TW; Sauder DN. 2000. CD4(+) Th1 and CD8(+) type 1 cytotoxic T cells both play a crucial role in the full development of contact hypersensitivity J Immunol 165(12):6783-90. [PubMed: 11120799]  [MGI Ref ID J:66170]

Wang C; Hino A; Yoshimoto T; Nagase H; Kato T; Hirokawa K; Matsuzawa A; Nariuchi H. 2000. Impaired delayed-type hypersensitivity response in mutant mice secreting soluble CD4 without expression of membrane-bound CD4 Immunology 100(3):309-16. [PubMed: 10929052]  [MGI Ref ID J:63431]

Wang T; Chen L; Ahmed E; Ma L; Yin D; Zhou P; Shen J; Xu H; Wang CR; Alegre ML; Chong AS. 2008. Prevention of allograft tolerance by bacterial infection with Listeria monocytogenes. J Immunol 180(9):5991-9. [PubMed: 18424719]  [MGI Ref ID J:134678]

Wen T; Trumper L; Fung-Leung W; Rahemtulla A; Klein E; Klein G; Mak TW. 1998. Requirement of the CD8+ or CD4+ T lymphocyte subsets for the rejection of lymphoma and fibrosarcoma grafts studied in gene knockout hosts. Immunol Lett 61(2-3):187-90. [PubMed: 9657273]  [MGI Ref ID J:47925]

Weng X; Priceputu E; Chrobak P; Poudrier J; Kay DG; Hanna Z; Mak TW; Jolicoeur P. 2004. CD4+ T cells from CD4C/HIVNef transgenic mice show enhanced activation in vivo with impaired proliferation in vitro but are dispensable for the development of a severe AIDS-like organ disease. J Virol 78(10):5244-57. [PubMed: 15113906]  [MGI Ref ID J:89588]

Wolfort RM; Stokes KY; Granger DN. 2008. CD4+ T lymphocytes mediate hypercholesterolemia-induced endothelial dysfunction via a NAD(P)H oxidase-dependent mechanism. Am J Physiol Heart Circ Physiol 294(6):H2619-26. [PubMed: 18408127]  [MGI Ref ID J:136828]

Xiang J; Huang H; Liu Y. 2005. A new dynamic model of CD8+ T effector cell responses via CD4+ T helper-antigen-presenting cells. J Immunol 174(12):7497-505. [PubMed: 15944248]  [MGI Ref ID J:100792]

Xin H; Kikuchi T; Andarini S; Ohkouchi S; Suzuki T; Nukiwa T; Hagiwara K; Honjo T; Saijo Y. 2005. Antitumor immune response by CX3CL1 fractalkine gene transfer depends on both NK and T cells. Eur J Immunol 35(5):1371-80. [PubMed: 15789339]  [MGI Ref ID J:97809]

Yusuf N; Nasti TH; Katiyar SK; Jacobs MK; Seibert MD; Ginsburg AC; Timares L; Xu H; Elmets CA. 2008. Antagonistic roles of CD4+ and CD8+ T-cells in 7,12-dimethylbenz(a)anthracene cutaneous carcinogenesis. Cancer Res 68(10):3924-30. [PubMed: 18483278]  [MGI Ref ID J:135019]

Zhang GX; Xiao BG; Bakhiet M; van der Meide P; Wigzell H; Link H; Olsson T. 1996. Both CD4+ and CD8+ T cells are essential to induce experimental autoimmune myasthenia gravis. J Exp Med 184(2):349-56. [PubMed: 8760788]  [MGI Ref ID J:110750]

Zhu Y; Bao L; Zhu S; Chen Z; van der Meide P; Nennesmo I; Winblad B; Ljunggren HG; Zhu J. 2002. CD4 and CD8 T cells, but not B cells, are critical to the control of murine experimental autoimmune neuritis. Exp Neurol 177(1):314-20. [PubMed: 12429233]  [MGI Ref ID J:118779]

de Visser KE; Korets LV; Coussens LM. 2005. De novo carcinogenesis promoted by chronic inflammation is B lymphocyte dependent. Cancer Cell 7(5):411-23. [PubMed: 15894262]  [MGI Ref ID J:98942]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX30

Colony Maintenance

Breeding & Husbandry	When maintaining a live colony, these mice can be bred as homozygotes. Expected coat color from breeding is black.
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes	Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.