Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S2 via D3 ES cell line Generation N13F?+27 (20-DEC-06)   Donating Investigator Tak Mak,   University Health Network/Un of Toronto

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Cd8a^tm1Mak targeted mutation are deficient in functional cytotoxic T-cells; however, helper T-cell development and function is comparable to normal.

Development
A targeting vector containing neomycin resistance gene was used to disrupt exon 1. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. Heterozygotes were intercrossed to generate homozygotes. The mice were then backcrossed to C57BL/6 for 13 generations before arriving at The Jackson Laboratory.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Cd8a^tm1Mak allele

002664	B6;129S-Cd4tm1Mak Cd8atm1Mak/J
007071	CByJ.129S2(B6)-Cd8atm1Mak/J
005513	NOD.129S2(B6)-Cd8atm1Mak/DvsJ

View Strains carrying   Cd8a^tm1Mak     (3 strains)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cd8a^tm1Mak/Cd8a^tm1Mak

        B6.129S2-Cd8a^tm1Mak

• immune system phenotype
• abnormal response to transplant (MGI Ref ID J:91742)
  • skin grafts from OVA expressing transgenic mice ( Tg(CAG-OVA)916Jen) shrink rapidly to 10-30% of their original size on mutant hosts but are then retained indefinitely in healthy condition

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd8a^tm1Mak/Cd8a^tm1Mak

        involves: 129S2/SvPas * C57BL/6 * DBA/2

• hematopoietic system phenotype
• absent CD8-positive T cells (MGI Ref ID J:68956)
• immune system phenotype
• abnormal cytotoxic T cell physiology (MGI Ref ID J:68956)
  • lacks cytotoxic T cell response
  • no response to class I MHC alloantigen but normal response to class II
• absent CD8-positive T cells (MGI Ref ID J:68956)
• increased susceptibility to viral infection (MGI Ref ID J:92227)
  • inflammation is mostly resolved by 45 days after TMEV infection on the resistant C57BL/6 background
  • infectious virus remains at 45 days, however, disease is absent by 10 months on the resistant C57BL/6 background
• nervous system phenotype
• demyelination (MGI Ref ID J:92227)
  • on the resistant C57BL/6 background, foci of demyelination at 45 days after TMEV infection

Cd8a^tm1Mak/Cd8a^tm1Mak

        P/J

• immune system phenotype
• increased susceptibility to viral infection (MGI Ref ID J:92227)
  • inflammation persists in the susceptible PL/J background
• nervous system phenotype
• demyelination (MGI Ref ID J:92227)
  • demyelination was severe on both the susceptible PL/J and SJL/J backgrounds after infection with TMEV

Cd8a^tm1Mak/Cd8a^tm1Mak

        SJL/J

• immune system phenotype
• increased susceptibility to viral infection (MGI Ref ID J:92227)
  • inflammation persists in the susceptible SJL/J background
• nervous system phenotype
• demyelination (MGI Ref ID J:92227)
  • demyelination was severe on both the susceptible PL/J and SJL/J backgrounds after infection with TMEV

Cd8a^tm1Mak/Cd8a^tm1Mak

        involves: 129S2/SvPas

• immune system phenotype
• *normal* immune system phenotype (MGI Ref ID J:110749)
  • mice clear polyomavirus within one month of infection similar to controls

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cd8a^tm1Mak related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cd8atm1Mak
Allele Name		targeted mutation 1, Tak Mak
Allele Type		Targeted (knock-out)
Common Name(s)		CD8 KO; CD8-; CD8-KO; CD8KO; CD8alpha-; CD8alpha-; Cd8atm1Mak; Lyt-2-;
Mutation Made By		Tak Mak,   University Health Network/Un of Toronto
Strain of Origin		129S2/SvPas
ES Cell Line Name		D3
ES Cell Line Strain		129S2/SvPas
Gene Symbol and Name		Cd8a, CD8 antigen, alpha chain
Chromosome		6
Gene Common Name(s)		BB154331; CD8; Leu2; Ly-2; Ly-35; Ly-B; Lyt-2; MAL; T-lymphocyte antigen 2; expressed sequence BB154331; lymphocyte antigen 2; lymphocyte antigen 35; p32;
Molecular Note		A neomycin resistance gene was inserted into exon 1. Flow cytometry analysis on thymus and lymph node cells derived from homozygous mice confirmed that no detectable encoded protein was expressed on the cell surface. [MGI Ref ID J:68956] [MGI Ref ID J:82297]

Genotyping

Genotyping Information

Genotyping Protocols

Cd8a^tm1Mak, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Fung-Leung WP; Schilham MW; Rahemtulla A; Kundig TM; Vollenweider M; Potter J; van Ewijk W; Mak TW. 1991. CD8 is needed for development of cytotoxic T cells but not helper T cells. Cell 65(3):443-9. [PubMed: 1673361]  [MGI Ref ID J:68956]

Additional References

Bitsaktsis C; Huntington J; Winslow G. 2004. Production of IFN-gamma by CD4 T cells is essential for resolving ehrlichia infection. J Immunol 172(11):6894-901. [PubMed: 15153508]  [MGI Ref ID J:90518]

Chiang EY; Stroynowski I. 2004. A nonclassical MHC class I molecule restricts CTL-mediated rejection of a syngeneic melanoma tumor. J Immunol 173(7):4394-401. [PubMed: 15383569]  [MGI Ref ID J:93729]

Chung DR; Kasper DL; Panzo RJ; Chtinis T; Grusby MJ; Sayegh MH; Tzianabos AO. 2003. CD4+ T cells mediate abscess formation in intra-abdominal sepsis by an IL-17-dependent mechanism. J Immunol 170(4):1958-63. [PubMed: 12574364]  [MGI Ref ID J:81809]

Fischbein MP; Yun J; Laks H; Irie Y; Fishbein MC; Espejo M; Bonavida B; Ardehali A. 2001. CD8+ lymphocytes augment chronic rejection in a MHC class II mismatched model. Transplantation 71(8):1146-53. [PubMed: 11374417]  [MGI Ref ID J:69459]

Koh DR; Fung-Leung WP; Ho A; Gray D; Acha-Orbea H; Mak TW. 1992. Less mortality but more relapses in experimental allergic encephalomyelitis in CD8-/- mice. Science 256(5060):1210-3. [PubMed: 1589800]  [MGI Ref ID J:1033]

Lambert SL; Okada CY; Levy R. 2004. TCR vaccines against a murine T cell lymphoma: a primary role for antibodies of the IgG2c class in tumor protection. J Immunol 172(2):929-36. [PubMed: 14707065]  [MGI Ref ID J:87361]

Le Moine A; Flamand V; de Lavareille A; Paulart F; Buonocore S; Vanderhaeghen ML; Nagy N; Habran C; Kiss R; Abramowicz D; Goldman M. 2002. Hypereosinophilic syndrome induced by neonatal immunization against MHC class II alloantigen: critical role of IL-4. Eur J Immunol 32(1):174-81. [PubMed: 11754358]  [MGI Ref ID J:73934]

Lewicki H; Tishon A; Homann D; Mazarguil H; Laval F; Asensio VC; Campbell IL; DeArmond S; Coon B; Teng C; Gairin JE; Oldstone MB. 2003. T cells infiltrate the brain in murine and human transmissible spongiform encephalopathies. J Virol 77(6):3799-808. [PubMed: 12610154]  [MGI Ref ID J:82227]

Liu N; Phillips T; Zhang M; Wang Y; Opferman JT; Shah R; Ashton-Rickardt PG. 2004. Serine protease inhibitor 2A is a protective factor for memory T cell development. Nat Immunol 5(9):919-26. [PubMed: 15311278]  [MGI Ref ID J:92217]

Mak TW; Rahemtulla A; Schilham M; Koh DR; Fung-Leung WP. 1992. Generation of mutant mice lacking surface expression of CD4 or CD8 by gene targeting. J Autoimmun 5 Suppl A:55-9. [PubMed: 1503636]  [MGI Ref ID J:575]

Moody AM; Chui D; Reche PA; Priatel JJ; Marth JD; Reinherz EL. 2001. Developmentally Regulated Glycosylation of the CD8alphabeta Coreceptor Stalk Modulates Ligand Binding. Cell 107(4):501-12. [PubMed: 11719190]  [MGI Ref ID J:72923]

Niethammer AG; Xiang R; Ruehlmann JM; Lode HN; Dolman CS; Gillies SD; Reisfeld RA. 2001. Targeted interleukin 2 therapy enhances protective immunity induced by an autologous oral DNA vaccine against murine melanoma. Cancer Res 61(16):6178-84. [PubMed: 11507070]  [MGI Ref ID J:71198]

Pien GC; Nguyen KB; Malmgaard L; Satoskar AR; Biron CA. 2002. A unique mechanism for innate cytokine promotion of T cell responses to viral infections. J Immunol 169(10):5827-37. [PubMed: 12421964]  [MGI Ref ID J:80075]

Ribas A; Wargo JA; Comin-Anduix B; Sanetti S; Schumacher LY; McLean C; Dissette VB; Glaspy JA; McBride WH; Butterfield LH; Economou JS. 2004. Enhanced tumor responses to dendritic cells in the absence of CD8-positive cells. J Immunol 172(8):4762-9. [PubMed: 15067052]  [MGI Ref ID J:89152]

Serizawa I; Koezuka Y; Amao H; Saito TR; Takahashi KW. 2000. Functional natural killer T cells in experimental mouse strains, including NK1.1- strains Exp Anim 49(3):171-80. [PubMed: 11109539]  [MGI Ref ID J:64139]

Urdahl KB; Liggitt D; Bevan MJ. 2003. CD8+ T cells accumulate in the lungs of Mycobacterium tuberculosis-infected Kb-/-Db-/- mice, but provide minimal protection. J Immunol 170(4):1987-94. [PubMed: 12574368]  [MGI Ref ID J:82297]

Cd8a^tm1Mak related

Alard P; Clark SL; Kosiewicz MM. 2004. Mechanisms of tolerance induced by TGF beta-treated APC: CD4 regulatory T cells prevent the induction of the immune response possibly through a mechanism involving TGF beta. Eur J Immunol 34(4):1021-30. [PubMed: 15048712]  [MGI Ref ID J:115475]

Antony PA; Piccirillo CA; Akpinarli A; Finkelstein SE; Speiss PJ; Surman DR; Palmer DC; Chan CC; Klebanoff CA; Overwijk WW; Rosenberg SA; Restifo NP. 2005. CD8+ T cell immunity against a tumor/self-antigen is augmented by CD4+ T helper cells and hindered by naturally occurring T regulatory cells. J Immunol 174(5):2591-601. [PubMed: 15728465]  [MGI Ref ID J:129825]

Bachmaier K; Neu N; Yeung RS; Mak TW; Liu P; Penninger JM. 1999. Generation of humanized mice susceptible to peptide-induced inflammatory heart disease. Circulation 99(14):1885-91. [PubMed: 10199887]  [MGI Ref ID J:129115]

Bachmann MF; Oxenius A; Mak TW; Zinkernagel RM. 1995. T cell development in CD8-/- mice. Thymic positive selection is biased toward the helper phenotype. J Immunol 155(8):3727-33. [PubMed: 7561076]  [MGI Ref ID J:29580]

Beilke JN; Kuhl NR; Van Kaer L; Gill RG. 2005. NK cells promote islet allograft tolerance via a perforin-dependent mechanism. Nat Med 11(10):1059-65. [PubMed: 16155578]  [MGI Ref ID J:101693]

Belkaid Y; Von Stebut E; Mendez S; Lira R; Caler E; Bertholet S; Udey MC; Sacks D. 2002. CD8+ T cells are required for primary immunity in C57BL/6 mice following low-dose, intradermal challenge with Leishmania major. J Immunol 168(8):3992-4000. [PubMed: 11937556]  [MGI Ref ID J:125458]

Ben-David H; Sharabi A; Dayan M; Sela M; Mozes E. 2007. The role of CD8+CD28 regulatory cells in suppressing myasthenia gravis-associated responses by a dual altered peptide ligand. Proc Natl Acad Sci U S A 104(44):17459-64. [PubMed: 17956982]  [MGI Ref ID J:127105]

Berke Z; Wen T; Jin S; Klein G; Dalianis T. 1995. Polyomavirus persists in CD4/8 double-knockout, but not in CD4 or CD8 single-knockout mice. Virology 212(1):268-71. [PubMed: 7676644]  [MGI Ref ID J:110749]

Berke Z; Wen T; Klein G; Dalianis T. 1996. Polyoma tumor development in neonatally polyoma-virus-infected CD4-/- and CD8-/- single knockout and CD4-/-8-/- double knockout mice. Int J Cancer 67(3):405-8. [PubMed: 8707416]  [MGI Ref ID J:113042]

Bitsaktsis C; Huntington J; Winslow G. 2004. Production of IFN-gamma by CD4 T cells is essential for resolving ehrlichia infection. J Immunol 172(11):6894-901. [PubMed: 15153508]  [MGI Ref ID J:90518]

Bitsaktsis C; Nandi B; Racine R; MacNamara KC; Winslow G. 2007. T-Cell-independent humoral immunity is sufficient for protection against fatal intracellular ehrlichia infection. Infect Immun 75(10):4933-41. [PubMed: 17664264]  [MGI Ref ID J:125283]

Bitsaktsis C; Winslow G. 2006. Fatal recall responses mediated by CD8 T cells during intracellular bacterial challenge infection. J Immunol 177(7):4644-51. [PubMed: 16982903]  [MGI Ref ID J:139316]

Bommireddy R; Engle SJ; Ormsby I; Boivin GP; Babcock GF; Doetschman T. 2004. Elimination of both CD4(+) and CD8(+) T cells but not B cells eliminates inflammation and prolongs the survival of TGFbeta1-deficient mice. Cell Immunol 232(1-2):96-104. [PubMed: 15922720]  [MGI Ref ID J:99033]

Borenstein SH; Graham J; Zhang XL; Chamberlain JW. 2000. CD8+ T cells are necessary for recognition of allelic, but not locus-mismatched or xeno-, HLA class I transplantation antigens. J Immunol 165(5):2341-53. [PubMed: 10946256]  [MGI Ref ID J:80678]

Bosselut R; Feigenbaum L; Sharrow SO; Singer A. 2001. Strength of signaling by CD4 and CD8 coreceptor tails determines the number but not the lineage direction of positively selected thymocytes. Immunity 14(4):483-94. [PubMed: 11336693]  [MGI Ref ID J:132432]

Bry L; Brenner MB. 2004. Critical role of T cell-dependent serum antibody, but not the gut-associated lymphoid tissue, for surviving acute mucosal infection with Citrobacter rodentium, an attaching and effacing pathogen. J Immunol 172(1):433-41. [PubMed: 14688352]  [MGI Ref ID J:87077]

Bumgardner GL; Gao D; Li J; Baskin JH; Heininger M; Orosz CG. 2000. Rejection responses to allogeneic hepatocytes by reconstituted SCID mice, CD4, KO, and CD8 KO mice. Transplantation 70(12):1771-80. [PubMed: 11152110]  [MGI Ref ID J:66571]

Burne MJ; Daniels F; El Ghandour A; Mauiyyedi S; Colvin RB; O'Donnell MP; Rabb H. 2001. Identification of the CD4(+) T cell as a major pathogenic factor in ischemic acute renal failure. J Clin Invest 108(9):1283-90. [PubMed: 11696572]  [MGI Ref ID J:118005]

Cardoso EM; Macedo MG; Rohrlich P; Ribeiro E; Silva MT; Lemonnier FA; de Sousa M. 2002. Increased hepatic iron in mice lacking classical MHC class I molecules. Blood 100(12):4239-41. [PubMed: 12393413]  [MGI Ref ID J:115559]

Chan WC; Duong TT; Yeung RS. 2004. Presence of IFN-gamma does not indicate its necessity for induction of coronary arteritis in an animal model of Kawasaki disease. J Immunol 173(5):3492-503. [PubMed: 15322214]  [MGI Ref ID J:92708]

Chen SY; Takeoka Y; Ansari AA; Boyd R; Klinman DM; Gershwin ME. 1996. The natural history of disease expression in CD4 and CD8 gene-deleted New Zealand black (NZB) mice. J Immunol 157(6):2676-84. [PubMed: 8805673]  [MGI Ref ID J:35433]

Chen Z; Yu S; Concha HQ; Zhu Y; Mix E; Winblad B; Ljunggren HG; Zhu J. 2004. Kainic acid-induced excitotoxic hippocampal neurodegeneration in C57BL/6 mice: B cell and T cell subsets may contribute differently to the pathogenesis. Brain Behav Immun 18(2):175-85. [PubMed: 14759595]  [MGI Ref ID J:105331]

Chiang EY; Stroynowski I. 2004. A nonclassical MHC class I molecule restricts CTL-mediated rejection of a syngeneic melanoma tumor. J Immunol 173(7):4394-401. [PubMed: 15383569]  [MGI Ref ID J:93729]

Ciurea A; Hunziker L; Klenerman P; Hengartner H; Zinkernagel RM. 2001. Impairment of CD4(+) T cell responses during chronic virus infection prevents neutralizing antibody responses against virus escape mutants. J Exp Med 193(3):297-305. [PubMed: 11157050]  [MGI Ref ID J:124426]

Crosby JR; Shen HH; Borchers MT; Justice JP; Ansay T; Lee JJ; Lee NA. 2002. Ectopic expression of IL-5 identifies an additional CD4(+) T cell mechanism of airway eosinophil recruitment. Am J Physiol Lung Cell Mol Physiol 282(1):L99-108. [PubMed: 11741821]  [MGI Ref ID J:107840]

D'Souza CD; Cooper AM; Frank AA; Ehlers S; Turner J; Bendelac A; Orme IM. 2000. A novel nonclassic beta2-microglobulin-restricted mechanism influencing early lymphocyte accumulation and subsequent resistance to tuberculosis in the lung. Am J Respir Cell Mol Biol 23(2):188-93. [PubMed: 10919985]  [MGI Ref ID J:114179]

Dalloul AH; Ngo K; Fung-Leung WP. 1996. CD4-negative cytotoxic T cells with a T cell receptor alpha/beta intermediate expression in CD8-deficient mice. Eur J Immunol 26(1):213-8. [PubMed: 8566069]  [MGI Ref ID J:112982]

Daniel D; Meyer-Morse N; Bergsland EK; Dehne K; Coussens LM; Hanahan D. 2003. Immune Enhancement of Skin Carcinogenesis by CD4+ T Cells. J Exp Med 197(8):1017-28. [PubMed: 12695493]  [MGI Ref ID J:82978]

Drake DR 3rd; Lukacher AE. 1998. Beta 2-microglobulin knockout mice are highly susceptible to polyoma virus tumorigenesis. Virology 252(1):275-84. [PubMed: 9875336]  [MGI Ref ID J:52185]

Duong TT; Silverman ED; Bissessar MV; Yeung RS. 2003. Superantigenic activity is responsible for induction of coronary arteritis in mice: an animal model of Kawasaki disease. Int Immunol 15(1):79-89. [PubMed: 12502728]  [MGI Ref ID J:81746]

Duthie MS; Kahn SJ. 2006. During acute Trypanosoma cruzi infection highly susceptible mice deficient in natural killer cells are protected by a single alpha-galactosylceramide treatment. Immunology 119(3):355-61. [PubMed: 16879622]  [MGI Ref ID J:118525]

Ehinger M; Vestberg M; Johansson AC; Johannesson M; Svensson A; Holmdahl R. 2001. Influence of CD4 or CD8 deficiency on collagen-induced arthritis. Immunology 103(3):291-300. [PubMed: 11454058]  [MGI Ref ID J:110421]

Ehst BD; Ingulli E; Jenkins MK. 2003. Development of a novel transgenic mouse for the study of interactions between CD4 and CD8 T cells during graft rejection. Am J Transplant 3(11):1355-62. [PubMed: 14525595]  [MGI Ref ID J:91742]

Elhage R; Gourdy P; Brouchet L; Jawien J; Fouque MJ; Fievet C; Huc X; Barreira Y; Couloumiers JC; Arnal JF; Bayard F. 2004. Deleting TCR alpha beta+ or CD4+ T lymphocytes leads to opposite effects on site-specific atherosclerosis in female apolipoprotein E-deficient mice. Am J Pathol 165(6):2013-8. [PubMed: 15579444]  [MGI Ref ID J:94976]

Enoh VT; Fairchild CD; Lin CY; Varma TK; Sherwood ER. 2006. Differential effect of imipenem treatment on wild-type and NK cell-deficient CD8 knockout mice during acute intra-abdominal injury. Am J Physiol Regul Integr Comp Physiol 290(3):R685-93. [PubMed: 16269570]  [MGI Ref ID J:105682]

Erman B; Alag AS; Dahle O; van Laethem F; Sarafova SD; Guinter TI; Sharrow SO; Grinberg A; Love PE; Singer A. 2006. Coreceptor signal strength regulates positive selection but does not determine CD4/CD8 lineage choice in a physiologic in vivo model. J Immunol 177(10):6613-25. [PubMed: 17082573]  [MGI Ref ID J:126026]

Evel-Kabler K; Song XT; Aldrich M; Huang XF; Chen SY. 2006. SOCS1 restricts dendritic cells' ability to break self tolerance and induce antitumor immunity by regulating IL-12 production and signaling. J Clin Invest 116(1):90-100. [PubMed: 16357940]  [MGI Ref ID J:105256]

Fang M; Sigal LJ. 2005. Antibodies and CD8+ T cells are complementary and essential for natural resistance to a highly lethal cytopathic virus. J Immunol 175(10):6829-36. [PubMed: 16272340]  [MGI Ref ID J:119697]

Fischbein MP; Ardehali A; Yun J; Schoenberger S; Laks H; Irie Y; Dempsey P; Cheng G; Fishbein MC; Bonavida B. 2000. CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants. J Immunol 165(12):7316-22. [PubMed: 11120867]  [MGI Ref ID J:118395]

Ford MS; Zhang ZX; Chen W; Zhang L. 2006. Double-negative T regulatory cells can develop outside the thymus and do not mature from CD8+ T cell precursors. J Immunol 177(5):2803-9. [PubMed: 16920915]  [MGI Ref ID J:139556]

Foucras G; Coudert JD; Coureau C; Guery JC. 2000. Dendritic cells prime in vivo alloreactive CD4 T lymphocytes toward type 2 cytokine- and TGF-beta-producing cells in the absence of CD8 T cell activation. J Immunol 165(9):4994-5003. [PubMed: 11046027]  [MGI Ref ID J:118923]

Fragoso RC; Pyarajan S; Irie HY; Burakoff SJ. 2006. A CD8/Lck transgene is able to drive thymocyte differentiation. J Immunol 177(9):6007-17. [PubMed: 17056525]  [MGI Ref ID J:140528]

Freland S; Ljunggren H. 2000. beta2-Microglobulin/CD8 -/- mice reveal significant role for CD8+ T cells in graft rejection responses in beta2-microglobulin -/- mice Scand J Immunol 51(3):219-23. [PubMed: 10736089]  [MGI Ref ID J:61231]

French JD; Roark CL; Born WK; O'brien RL. 2005. {gamma}{delta} T cell homeostasis is established in competition with {alpha}{beta} T cells and NK cells. Proc Natl Acad Sci U S A 102(41):14741-6. [PubMed: 16203967]  [MGI Ref ID J:102497]

Fung-Leung WP; Louie MC; Limmer A; Ohashi PS; Ngo K; Chen L; Kawai K; Lacy E; Loh DY; Mak TW. 1993. The lack of CD8 alpha cytoplasmic domain resulted in a dramatic decrease in efficiency in thymic maturation but only a moderate reduction in cytotoxic function of CD8+ T lymphocytes. Eur J Immunol 23(11):2834-40. [PubMed: 8223860]  [MGI Ref ID J:113044]

Fung-Leung WP; Wallace VA; Gray D; Sha WC; Pircher H; Teh HS; Loh DY; Mak TW. 1993. CD8 is needed for positive selection but differentially required for negative selection of T cells during thymic ontogeny. Eur J Immunol 23(1):212-6. [PubMed: 8419174]  [MGI Ref ID J:112993]

Gao Y; Tao J; Li MO; Zhang D; Chi H; Henegariu O; Kaech SM; Davis RJ; Flavell RA; Yin Z. 2005. JNK1 is essential for CD8+ T cell-mediated tumor immune surveillance. J Immunol 175(9):5783-9. [PubMed: 16237070]  [MGI Ref ID J:119340]

Goldszmid RS; Bafica A; Jankovic D; Feng CG; Caspar P; Winkler-Pickett R; Trinchieri G; Sher A. 2007. TAP-1 indirectly regulates CD4+ T cell priming in Toxoplasma gondii infection by controlling NK cell IFN-{gamma} production. J Exp Med 204(11):2591-602. [PubMed: 17923502]  [MGI Ref ID J:126112]

Gorbachev AV; Fairchild RL. 2004. CD40 engagement enhances antigen-presenting langerhans cell priming of IFN-gamma-producing CD4+ and CD8+ T cells independently of IL-12. J Immunol 173(4):2443-52. [PubMed: 15294958]  [MGI Ref ID J:92689]

Gramaglia I; Sahlin H; Nolan JP; Frangos JA; Intaglietta M; van der Heyde HC. 2005. Cell- rather than antibody-mediated immunity leads to the development of profound thrombocytopenia during experimental Plasmodium berghei malaria. J Immunol 175(11):7699-707. [PubMed: 16301680]  [MGI Ref ID J:122163]

Grayson MH; Cheung D; Rohlfing MM; Kitchens R; Spiegel DE; Tucker J; Battaile JT; Alevy Y; Yan L; Agapov E; Kim EY; Holtzman MJ. 2007. Induction of high-affinity IgE receptor on lung dendritic cells during viral infection leads to mucous cell metaplasia. J Exp Med 204(11):2759-69. [PubMed: 17954569]  [MGI Ref ID J:126124]

Grdic D; Hornquist E; Kjerrulf M; Lycke NY. 1998. Lack of local suppression in orally tolerant CD8-deficient mice reveals a critical regulatory role of CD8+ T cells in the normal gut mucosa. J Immunol 160(2):754-62. [PubMed: 9551910]  [MGI Ref ID J:45177]

Guleria I; Gubbels Bupp M; Dada S; Fife B; Tang Q; Ansari MJ; Trikudanathan S; Vadivel N; Fiorina P; Yagita H; Azuma M; Atkinson M; Bluestone JA; Sayegh MH. 2007. Mechanisms of PDL1-mediated regulation of autoimmune diabetes. Clin Immunol 125(1):16-25. [PubMed: 17627890]  [MGI Ref ID J:125272]

Hancock WW; Tsai TL; Madaio MP; Gasser DL. 2003. Cutting Edge: Multiple autoimmune pathways in kd/kd mice. J Immunol 171(6):2778-81. [PubMed: 12960297]  [MGI Ref ID J:85380]

He X; He X; Dave VP; Zhang Y; Hua X; Nicolas E; Xu W; Roe BA; Kappes DJ. 2005. The zinc finger transcription factor Th-POK regulates CD4 versus CD8 T-cell lineage commitment. Nature 433(7028):826-33. [PubMed: 15729333]  [MGI Ref ID J:95867]

Hegde S; Niederkorn JY. 2000. The role of cytotoxic T lymphocytes in corneal allograft rejection. Invest Ophthalmol Vis Sci 41(11):3341-7. [PubMed: 11006223]  [MGI Ref ID J:115389]

Hida S; Ogasawara K; Sato K; Abe M; Takayanagi H; Yokochi T; Sato T; Hirose S; Shirai T; Taki S; Taniguchi T. 2000. CD8(+) T cell-mediated skin disease in mice lacking IRF-2, the transcriptional attenuator of interferon-alpha/beta signaling Immunity 13(5):643-55. [PubMed: 11114377]  [MGI Ref ID J:66034]

Hornquist CE; Ekman L; Grdic KD; Schon K; Lycke NY. 1995. Paradoxical IgA immunity in CD4-deficient mice. Lack of cholera toxin-specific protective immunity despite normal gut mucosal IgA differentiation. J Immunol 155(6):2877-87. [PubMed: 7673704]  [MGI Ref ID J:110845]

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Wang T; Chen L; Ahmed E; Ma L; Yin D; Zhou P; Shen J; Xu H; Wang CR; Alegre ML; Chong AS. 2008. Prevention of allograft tolerance by bacterial infection with Listeria monocytogenes. J Immunol 180(9):5991-9. [PubMed: 18424719]  [MGI Ref ID J:134678]

Wei B; Su TT; Dalwadi H; Stephan RP; Fujiwara D; Huang TT; Brewer S; Chen L; Arditi M; Borneman J; Rawlings DJ; Braun J. 2008. Resident enteric microbiota and CD8(+) T cells shape the abundance of marginal zone B cells. Eur J Immunol 38(12):3411-3425. [PubMed: 19009526]  [MGI Ref ID J:141389]

Wen T; Trumper L; Fung-Leung W; Rahemtulla A; Klein E; Klein G; Mak TW. 1998. Requirement of the CD8+ or CD4+ T lymphocyte subsets for the rejection of lymphoma and fibrosarcoma grafts studied in gene knockout hosts. Immunol Lett 61(2-3):187-90. [PubMed: 9657273]  [MGI Ref ID J:47925]

Wesche-Soldato DE; Chung CS; Gregory SH; Salazar-Mather TP; Ayala CA; Ayala A. 2007. CD8+ T cells promote inflammation and apoptosis in the liver after sepsis: role of Fas-FasL. Am J Pathol 171(1):87-96. [PubMed: 17591956]  [MGI Ref ID J:122836]

Wolfort RM; Stokes KY; Granger DN. 2008. CD4+ T lymphocytes mediate hypercholesterolemia-induced endothelial dysfunction via a NAD(P)H oxidase-dependent mechanism. Am J Physiol Heart Circ Physiol 294(6):H2619-26. [PubMed: 18408127]  [MGI Ref ID J:136828]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX30

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply	Level 4. Up to 10 mice. Larger quantities or custom orders arranged upon request. Expected delivery up to one to three months.
Supply Notes	Shipped at a specific age in weeks. Mice at a precise age in days, littermates and retired breeders are also available. Strains that must be genotyped are not available until five to seven weeks of age. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.