Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.PERA-Tnfsf4Ath1-PERA/EiJ    (Changed: 16-NOV-05 ) B6.PERA-Ath1r    (Changed: 06-MAY-05 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6J Donor Strain PERA/Ei Generation N10F10+1p Generation Definitions

Appearance
black
Related Genotype: a/a

Description
This strain carries the Tnfsf4^{Ath1-PERA/EiJ} resistance allele of the wild-derived strain PERA, which confers a high serum HDL cholesterol level and resistance to the development of atherosclerosis when the mice are fed a high fat diet on the C57BL/6J strain background. The C57BL/6J allele results in low HDL cholesterol levels and susceptibility to diet induced atherosclerosis.

Development
Mice of the strain PERA, descended from wild mice trapped in Peru, are resistant to atherosclerosis and have elevated HDL levels. A congenic strain was constructed by crossing a PERA mouse to a C57BL/6J mouse, then selecting mice having high HDL levels for each subsequent backcross to C57BL/6J. At N5, the high HDL/ atherosclerosis resistance trait was mapped and found to localize to the same genomic position as Ath1. The congenic has now been backcrossed for ten generations. (The PERA subline used to construct this strain is now extinct.) (Paigen, 2000)

B6.PERA-Tnfsf4^{Ath1-PERA/EiJ} was transfered to the production colony at JAX from Dr. Beverly Paigen's colony, also at JAX. The PERA/Ei inbred used as the donor strain in this congenic originally came from Peru. The original was trapped by Olga Atteck for M.E. Wallace, University of Cambridge, in 1961 in a Peruvian Yard used to dry maize cobs in Nana Village, Rimac Valley, Peru. The mice were held in a small, closed colony by Wallace. In 1971, at approximately F25, mice from his colony were sent to E.M. Eicher and T.H. Roderick at JAX. PERA/Ei and PERA/Rk were established immediatelly as separate colonies.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tnfsf4

005969	B6Cr.129S4-Tnfsf4tm1Sug/Pgn
005967	C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn
005968	C57BL/6J-Tg(Mt1-Tnfsf4)2Pgn/Pgn
005075	NOD.129(B6)-Tnfsf4tm1Shr/DoiJ

View Strains carrying other alleles of Tnfsf4     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI

      assigned by genotype

Tnfsf4^{Ath1-PERA/EiJ}/Tnfsf4^{Ath1-PERA/EiJ}

        B6.PERA-Tnfsf4^{Ath1-PERA/EiJ}/Pgn

• homeostasis/metabolism phenotype
• increased circulating HDL cholesterol level
  • HDL cholesterol levels are higher in mice homozygous for the PERA/EiJ allele than in homozygotes for the C57BL/6J allele   (MGI Ref ID J:79722)
  • homozygotes for the PERA allele maintain high HDL cholesterol levels when fed a high-fat diet   (MGI Ref ID J:79722)
• cardiovascular system phenotype
• abnormal blood vessel morphology
  • homozygous mice develop smaller aortic fatty streaks than do C57BL/6J control mice when fed a high-fat diet   (MGI Ref ID J:79722)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Tnfsf4^{Ath1-PERA/EiJ} related

Cardiovascular Research
Atherosclerosis
Diet-Induced Atherosclerosis

Genes & Alleles

Gene & Allele Information provided by MGI

Allele Symbol		Tnfsf4Ath1-PERA/EiJ
Allele Name		PERA/EiJ
Allele Type		QTL
Common Name(s)		Ath1r;
Strain of Origin		PERA/EiJ
Gene Symbol and Name		Tnfsf4, tumor necrosis factor (ligand) superfamily, member 4
Chromosome		1
Gene Common Name(s)		Ath-1; Ath1; CD134L; CD252; GP34; OX-40L; OX40L; OX4OL; Ox40 ligand; Ox40l; TXGP1; Txgp1l; atherosclerosis 1; tax-transcriptionally activated glycoprotein 1 ligand;
Molecular Note		This allele confers elevated high-density lipoprotein (HDL) cholesterol levels and resistance to diet-induced atherosclerosis relative to the C57BL/6J allele.

Genotyping

Genotyping Information

Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Paigen B. 1995. Genetics of responsiveness to high-fat and high- cholesterol diets in the mouse. Am J Clin Nutr 62(2):458S-462S. [PubMed: 7625360]  [MGI Ref ID J:28248]

Paigen B; Albee D; Holmes PA; Mitchell D. 1987. Genetic analysis of murine strains C57BL/6J and C3H/HeJ to confirm the map position of Ath-1, a gene determining atherosclerosis susceptibility. Biochem Genet 25(7-8):501-11. [PubMed: 3128974]  [MGI Ref ID J:9135]

Paigen B; Mitchell D; Holmes PA; Albee D. 1987. Genetic analysis of strains C57BL/6J and BALB/cJ for Ath-1, a gene determining atherosclerosis susceptibility in mice. Biochem Genet 25(11-12):881-92. [PubMed: 3450276]  [MGI Ref ID J:9185]

Paigen B; Mitchell D; Reue K; Morrow A; Lusis AJ; LeBoeuf RC. 1987. Ath-1, a gene determining atherosclerosis susceptibility and high density lipoprotein levels in mice. Proc Natl Acad Sci U S A 84(11):3763-7. [PubMed: 3473481]  [MGI Ref ID J:8735]

Tnfsf4^{Ath1-PERA/EiJ} related

Phelan SA; Beier DR; Higgins DC; Paigen B. 2002. Confirmation and high resolution mapping of an atherosclerosis susceptibility gene in mice on Chromosome 1. Mamm Genome 13(10):548-53. [PubMed: 12420131]  [MGI Ref ID J:79722]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Purchasing information

Pricing, Supply Level & Notes, Controls

General Supply Notes

• Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
Control Pricing Information for Genetically Engineered Mutant Strains.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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