Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.PERA-Tnfsf4Ath1-PERA/EiJ    (Changed: 16-NOV-05 ) B6.PERA-Ath1r    (Changed: 06-MAY-05 ) Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6J Donor Strain PERA/Ei Generation N10F10+1p

Appearance
black
Related Genotype: a/a

Description
This strain carries the Tnfsf4^{Ath1-PERA/EiJ} resistance allele of the wild-derived strain PERA, which confers a high serum HDL cholesterol level and resistance to the development of atherosclerosis when the mice are fed a high fat diet on the C57BL/6J strain background. The C57BL/6J allele results in low HDL cholesterol levels and susceptibility to diet induced atherosclerosis.

Development
Mice of the strain PERA, descended from wild mice trapped in Peru, are resistant to atherosclerosis and have elevated HDL levels. A congenic strain was constructed by crossing a PERA mouse to a C57BL/6J mouse, then selecting mice having high HDL levels for each subsequent backcross to C57BL/6J. At N5, the high HDL/ atherosclerosis resistance trait was mapped and found to localize to the same genomic position as Ath1. The congenic has now been backcrossed for ten generations. (The PERA subline used to construct this strain is now extinct.) (Paigen, 2000)

B6.PERA-Tnfsf4^{Ath1-PERA/EiJ} was transfered to the production colony at JAX from Dr. Beverly Paigen's colony, also at JAX. The PERA/Ei inbred used as the donor strain in this congenic originally came from Peru. The original was trapped by Olga Atteck for M.E. Wallace, University of Cambridge, in 1961 in a Peruvian Yard used to dry maize cobs in Nana Village, Rimac Valley, Peru. The mice were held in a small, closed colony by Wallace. In 1971, at approximately F25, mice from his colony were sent to E.M. Eicher and T.H. Roderick at JAX. PERA/Ei and PERA/Rk were established immediatelly as separate colonies.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Tnfsf4

005969	B6Cr.129S4-Tnfsf4tm1Sug/Pgn
005967	C57BL/6J-Tg(Mt1-Tnfsf4)1Pgn/Pgn
005968	C57BL/6J-Tg(Mt1-Tnfsf4)2Pgn/Pgn
005075	NOD.129(B6)-Tnfsf4tm1Shr/DoiJ

View Strains carrying other alleles of Tnfsf4     (4 strains)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tnfsf4^{Ath1-PERA/EiJ}/Tnfsf4^{Ath1-PERA/EiJ}

        B6.PERA-Tnfsf4^{Ath1-PERA/EiJ}/Pgn

• homeostasis/metabolism phenotype
• increased circulating HDL cholesterol level (MGI Ref ID J:79722)
  • HDL cholesterol levels are higher in mice homozygous for the PERA/EiJ allele than in homozygotes for the C57BL/6J allele
  • homozygotes for the PERA allele maintain high HDL cholesterol levels when fed a high-fat diet
• cardiovascular system phenotype
• abnormal vasculature (MGI Ref ID J:79722)
  • homozygous mice develop smaller aortic fatty streaks than do C57BL/6J control mice when fed a high-fat diet

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Tnfsf4^{Ath1-PERA/EiJ} related

Cardiovascular Research
Atherosclerosis
Diet-Induced Atherosclerosis

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tnfsf4Ath1-PERA/EiJ
Allele Name		PERA/EiJ
Allele Type		QTL
Common Name(s)		Ath1r;
Strain of Origin		PERA/EiJ
Gene Symbol and Name		Tnfsf4, tumor necrosis factor (ligand) superfamily, member 4
Chromosome		1
Gene Common Name(s)		Ath-1; Ath1; CD134L; CD252; GP34; OX-40L; OX40L; OX4OL; Ox40 ligand; Ox40l; TXGP1; Txgp1l; atherosclerosis 1; tax-transcriptionally activated glycoprotein 1 ligand;
Molecular Note		This allele confers elevated high-density lipoprotein (HDL) cholesterol levels and resistance to diet-induced atherosclerosis relative to the C57BL/6J allele.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Additional References

Paigen B. 1995. Genetics of responsiveness to high-fat and high- cholesterol diets in the mouse. Am J Clin Nutr 62(2):458S-462S. [PubMed: 7625360]  [MGI Ref ID J:28248]

Paigen B; Albee D; Holmes PA; Mitchell D. 1987. Genetic analysis of murine strains C57BL/6J and C3H/HeJ to confirm the map position of Ath-1, a gene determining atherosclerosis susceptibility. Biochem Genet 25(7-8):501-11. [PubMed: 3128974]  [MGI Ref ID J:9135]

Paigen B; Mitchell D; Holmes PA; Albee D. 1987. Genetic analysis of strains C57BL/6J and BALB/cJ for Ath-1, a gene determining atherosclerosis susceptibility in mice. Biochem Genet 25(11-12):881-92. [PubMed: 3450276]  [MGI Ref ID J:9185]

Paigen B; Mitchell D; Reue K; Morrow A; Lusis AJ; LeBoeuf RC. 1987. Ath-1, a gene determining atherosclerosis susceptibility and high density lipoprotein levels in mice. Proc Natl Acad Sci U S A 84(11):3763-7. [PubMed: 3473481]  [MGI Ref ID J:8735]

Tnfsf4^{Ath1-PERA/EiJ} related

Phelan SA; Beier DR; Higgins DC; Paigen B. 2002. Confirmation and high resolution mapping of an atherosclerosis susceptibility gene in mice on Chromosome 1. Mamm Genome 13(10):548-53. [PubMed: 12420131]  [MGI Ref ID J:79722]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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