Strain Name:

FVB.Cg-Tg(WapMyc)212Bri/J

Stock Number:

002677

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names FVB/N-Tg(WapMyc)212Bri/J    (Changed: 08-AUG-08 )
Type Congenic; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
 
Donating InvestigatorDr. Eric Sandgren,   Univ of Wisconsin School of Vet Med

Description
Female mice transgenic for the Tg(WapMyc)212Bri are viable and fertile but do not lactate. Mammary glands from pregnant transgenic mice showed enlarged and hyperplastic alveoli with poorly differentiated epithelial cells. Multiparous females develop observable mammary tumors at the average age of 6 months. The majority of tumors were of mixed histotypes with solid as well as glandular or papillary foci. Lung metastases were observed in 3 of 14 tumor-bearing mice. All tumors tested were transplantable into syngeneic mice.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Myc     (15 strains)

Strains carrying other alleles of Wap
002499   C57BL/6J-Tg(WapIGFBP3)67Dlr/J
002659   FVB/N-Tg(Trp53R172H)8512Jmr/J
002660   FVB/N-Tg(Trp53R172L)4491Jmr/J
View Strains carrying other alleles of Wap     (3 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on transgenic expression of a human gene that is associated with this disease. Phenotypic similarity to the human disease has not been tested.
Burkitt Lymphoma; BL   (MYC)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(WapMyc)212Bri/0

        FVB.Cg-Tg(WapMyc)212Bri
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland morphology
    • apoptosis in the mammary gland does not decrease with age   (MGI Ref ID J:114568)
    • abnormal mammary gland lobule morphology
      • lobules contain more cells than in wild-type mice   (MGI Ref ID J:114568)
    • mammary gland duct hyperplasia
      • ductal cell proliferation rates are higher than in wild-type mice but equivalent to lobule proliferation rates   (MGI Ref ID J:114568)
    • mammary gland hyperplasia
      • cell proliferation within lobules is increased after 16 weeks of age compared to wild-type mice   (MGI Ref ID J:114568)
      • unlike wild-type mice, proliferation rates remain high as mice age   (MGI Ref ID J:114568)
      • mice exhibit increased number and size of hyperplastic areas compared to wild-type mice   (MGI Ref ID J:114568)
      • cell proliferation of normal cells in tumor containing glands is increased compared to non-tumor containing glands   (MGI Ref ID J:114568)
  • tumorigenesis
  • increased mammary gland tumor incidence
    • cell proliferation rates within tumors is greater than in hyperplastic mammary tissues   (MGI Ref ID J:114568)
  • integument phenotype
  • abnormal mammary gland morphology
    • apoptosis in the mammary gland does not decrease with age   (MGI Ref ID J:114568)
    • abnormal mammary gland lobule morphology
      • lobules contain more cells than in wild-type mice   (MGI Ref ID J:114568)
    • mammary gland duct hyperplasia
      • ductal cell proliferation rates are higher than in wild-type mice but equivalent to lobule proliferation rates   (MGI Ref ID J:114568)
    • mammary gland hyperplasia
      • cell proliferation within lobules is increased after 16 weeks of age compared to wild-type mice   (MGI Ref ID J:114568)
      • unlike wild-type mice, proliferation rates remain high as mice age   (MGI Ref ID J:114568)
      • mice exhibit increased number and size of hyperplastic areas compared to wild-type mice   (MGI Ref ID J:114568)
      • cell proliferation of normal cells in tumor containing glands is increased compared to non-tumor containing glands   (MGI Ref ID J:114568)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Tg(WapMyc)212Bri/0

        involves: C57BL/6 * SJL
  • mortality/aging
  • premature death
    • female mice die by 8 months of age   (MGI Ref ID J:56318)
  • endocrine/exocrine gland phenotype
  • abnormal lactation
    • female mice do not lactate   (MGI Ref ID J:56318)
  • mammary gland alveolar hyperplasia
    • female mice exhibit enlarged and hyperplastic alveoli at day 14 of pregnancy with poorly differentiated epithelium and numerous dead cells   (MGI Ref ID J:56318)
  • tumorigenesis
  • increased mammary gland tumor incidence
    • female mice develop mammary gland tumors and die by 8 months of age   (MGI Ref ID J:56318)
    • female mice develop solid carcinomas and few glandular/microcystic or papillary adenocarcinomas   (MGI Ref ID J:56318)
    • 42% of tumors are mixed glandular and solid or glandular and papillary   (MGI Ref ID J:56318)
    • lung metastasis occurs in 3 of 14 mice and tumors are transplantable   (MGI Ref ID J:56318)
    • however, virgin females do not develop mammary gland tumors   (MGI Ref ID J:56318)
    • increased mammary adenocarcinoma incidence
      • few tumors are adenocarcinomas   (MGI Ref ID J:56318)
  • integument phenotype
  • abnormal lactation
    • female mice do not lactate   (MGI Ref ID J:56318)
  • mammary gland alveolar hyperplasia
    • female mice exhibit enlarged and hyperplastic alveoli at day 14 of pregnancy with poorly differentiated epithelium and numerous dead cells   (MGI Ref ID J:56318)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Mammary Gland Tumors
Oncogenes

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(WapMyc)212Bri
Allele Name transgene insertion 212, Ralph L Brinster
Allele Type Transgenic (Inserted expressed sequence)
Mutation Made ByDr. Ralph Brinster,   University of Pennsylvania
Strain of Origin(C57BL/6 x SJL)F2
Expressed Gene Myc, myelocytomatosis oncogene, mouse, laboratory
Promoter Wap, whey acidic protein, rat
Molecular Note The transgene contains the mouse whey acidic protein (WAP) promoter and the mouse myelocytomatosis oncogene (Myc). [MGI Ref ID J:56318]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(WapMyc)212Bri, Separated PCR
Tg(WapMyc)212Bri, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Sandgren EP; Schroeder JA; Qui TH; Palmiter RD; Brinster RL; Lee DC. 1995. Inhibition of mammary gland involution is associated with transforming growth factor alpha but not c-myc-induced tumorigenesis in transgenic mice. Cancer Res 55(17):3915-27. [PubMed: 7641211]  [MGI Ref ID J:56318]

Additional References

Tg(WapMyc)212Bri related

Herschkowitz JI; Simin K; Weigman VJ; Mikaelian I; Usary J; Hu Z; Rasmussen KE; Jones LP; Assefnia S; Chandrasekharan S; Backlund MG; Yin Y; Khramtsov AI; Bastein R; Quackenbush J; Glazer RI; Brown PH; Green JE; Kopelovich L; Furth PA; Palazzo JP; Olopade OI; Bernard PS; Churchill GA; Van Dyke T; Perou CM. 2007. Identification of conserved gene expression features between murine mammary carcinoma models and human breast tumors. Genome Biol 8(5):R76. [PubMed: 17493263]  [MGI Ref ID J:160877]

Kundel DW; Stromquist E; Greene AL; Zhdankin O; Regal RR; Rose-Hellekant TA. 2012. Molecular characterizations of Nop16 in murine mammary tumors with varying levels of c-Myc. Transgenic Res 21(2):393-406. [PubMed: 21863248]  [MGI Ref ID J:183571]

Mikaelian I; Blades N; Churchill GA; Fancher K; Knowles BB; Eppig JT; Sundberg JP. 2004. Proteotypic classification of spontaneous and transgenic mammary neoplasms. Breast Cancer Res 6(6):R668-79. [PubMed: 15535849]  [MGI Ref ID J:94093]

Nieminen AI; Eskelinen VM; Haikala HM; Tervonen TA; Yan Y; Partanen JI; Klefstrom J. 2013. Myc-induced AMPK-phospho p53 pathway activates Bak to sensitize mitochondrial apoptosis. Proc Natl Acad Sci U S A 110(20):E1839-48. [PubMed: 23589839]  [MGI Ref ID J:197360]

Rose-Hellekant TA; Wentworth KM; Nikolai S; Kundel DW; Sandgren EP. 2006. Mammary Carcinogenesis Is Preceded by Altered Epithelial Cell Turnover in Transforming Growth Factor-{alpha} and c-myc Transgenic Mice. Am J Pathol 169(5):1821-32. [PubMed: 17071603]  [MGI Ref ID J:114568]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We willfulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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