Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names FVB/N-Tg(WapMyc)212Bri/J    (Changed: 08-AUG-08 ) Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N18   Donating Investigator Eric Sandgren,   Univ of Wisconsin School of Vet Med

Description
Female mice transgenic for the Tg(WapMyc)212Bri are viable and fertile but do not lactate. Mammary glands from pregnant transgenic mice showed enlarged and hyperplastic alveoli with poorly differentiated epithelial cells. Multiparous females develop observable mammary tumors at the average age of 6 months. The majority of tumors were of mixed histotypes with solid as well as glandular or papillary foci. Lung metastases were observed in 3 of 14 tumor-bearing mice. All tumors tested were transplantable into syngeneic mice.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Myc

007693	B6(Cg)-Myctm37Mnz/J
007692	B6(Cg)-Myctm39Mnz/J
002728	B6.Cg-Tg(IghMyc)22Bri/J

View Strains carrying other alleles of Myc     (3 strains)

Strains carrying other alleles of Wap

007962	B6.FVB-Tg(MMTV-neu/OT-I/OT-II)CBnel Tg(Trp53R172H)8512Jmr/J
002499	C57BL/6J-Tg(WapIGFBP3)67Dlr/J
002659	FVB/N-Tg(Trp53R172H)8512Jmr/J
002660	FVB/N-Tg(Trp53R172L)4491Jmr/J

View Strains carrying other alleles of Wap     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(WapMyc)212Bri/0

        FVB.Cg-Tg(WapMyc)212Bri

• endocrine/exocrine gland phenotype
• abnormal mammary gland morphology (MGI Ref ID J:114568)
  • apoptosis in the mammary gland does not decrease with age
• abnormal mammary gland lobule morphology (MGI Ref ID J:114568)
  • lobules contain more cells than in wild-type mice
• mammary gland duct hyperplasia (MGI Ref ID J:114568)
  • ductal cell proliferation rates are higher than in wild-type mice but equivalent to lobule proliferation rates
• mammary gland hyperplasia (MGI Ref ID J:114568)
  • cell proliferation within lobules is increased after 16 weeks of age compared to wild-type mice
  • unlike wild-type mice, proliferation rates remain high as mice age
  • mice exhibit increased number and size of hyperplastic areas compared to wild-type mice
  • cell proliferation of normal cells in tumor containing glands is increased compared to non-tumor containing glands
• tumorigenesis
• mammary gland tumor (MGI Ref ID J:114568)
  • cell proliferation rates within tumors is greater than in hyperplastic mammary tissues
• reproductive system phenotype
• abnormal mammary gland morphology (MGI Ref ID J:114568)
  • apoptosis in the mammary gland does not decrease with age
• abnormal mammary gland lobule morphology (MGI Ref ID J:114568)
  • lobules contain more cells than in wild-type mice
• mammary gland duct hyperplasia (MGI Ref ID J:114568)
  • ductal cell proliferation rates are higher than in wild-type mice but equivalent to lobule proliferation rates
• mammary gland hyperplasia (MGI Ref ID J:114568)
  • cell proliferation within lobules is increased after 16 weeks of age compared to wild-type mice
  • unlike wild-type mice, proliferation rates remain high as mice age
  • mice exhibit increased number and size of hyperplastic areas compared to wild-type mice
  • cell proliferation of normal cells in tumor containing glands is increased compared to non-tumor containing glands

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(WapMyc)212Bri/0

        involves: C57BL/6 * SJL

• life span-post-weaning/aging
• premature death (MGI Ref ID J:56318)
  • female mice die by 8 months of age
• endocrine/exocrine gland phenotype
• abnormal lactation (MGI Ref ID J:56318)
  • female mice do not lactate
• mammary gland alveolar hyperplasia (MGI Ref ID J:56318)
  • female mice exhibit enlarged and hyperplastic alveoli at day 14 of pregnancy with poorly differentiated epithelium and numerous dead cells
• tumorigenesis
• mammary gland tumor (MGI Ref ID J:56318)
  • female mice develop mammary gland tumors and die by 8 months of age
  • female mice develop solid carcinomas and few glandular/microcystic or papillary adenocarcinomas
  • 42% of tumors are mixed glandular and solid or glandular and papillary
  • lung metastasis occurs in 3 of 14 mice and tumors are transplantable
  • however, virgin females do not develop mammary gland tumors
• mammary adenocarcinoma (MGI Ref ID J:56318)
  • few tumors are adenocarcinomas
• reproductive system phenotype
• abnormal lactation (MGI Ref ID J:56318)
  • female mice do not lactate
• mammary gland alveolar hyperplasia (MGI Ref ID J:56318)
  • female mice exhibit enlarged and hyperplastic alveoli at day 14 of pregnancy with poorly differentiated epithelium and numerous dead cells

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Mammary Gland Tumors
Oncogenes

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(WapMyc)212Bri
Allele Name		transgene insertion 212, Ralph L Brinster
Allele Type		Transgenic (random, expressed)
Mutation Made By		Ralph Brinster,   University of Pennsylvania
Strain of Origin		(C57BL/6 x SJL)F2
Expressed Gene		Myc, myelocytomatosis oncogene, mouse, laboratory
Promoter		Wap, whey acidic protein, rat
Molecular Note		The transgene contains the mouse whey acidic protein (WAP) promoter and the mouse myelocytomatosis oncogene (Myc). [MGI Ref ID J:56318]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Sandgren EP; Schroeder JA; Qui TH; Palmiter RD; Brinster RL; Lee DC. 1995. Inhibition of mammary gland involution is associated with transforming growth factor alpha but not c-myc-induced tumorigenesis in transgenic mice. Cancer Res 55(17):3915-27. [PubMed: 7641211]  [MGI Ref ID J:56318]

Additional References

Tg(WapMyc)212Bri related

Rose-Hellekant TA; Wentworth KM; Nikolai S; Kundel DW; Sandgren EP. 2006. Mammary Carcinogenesis Is Preceded by Altered Epithelial Cell Turnover in Transforming Growth Factor-{alpha} and c-myc Transgenic Mice. Am J Pathol 169(5):1821-32. [PubMed: 17071603]  [MGI Ref ID J:114568]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

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Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

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"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

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