Strain Name:

FVB.Cg-Tg(WapTgfa)215Bri/J

Stock Number:

002678

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Availability:

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names FVB/N-Tg(WapTgfa)215Bri/J    (Changed: 08-AUG-08 )
Type Congenic; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Specieslaboratory mouse
GenerationN12
Generation Definitions
 
Donating InvestigatorDr. Eric Sandgren,   Univ of Wisconsin School of Vet Med

Description
Transgenic females showed precocious mammary gland development during pregnancy. Involution was delayed beyond 8 days post-parturition. With successive pregnancies hyperplastic alveolar nodules increased in number. Mammary tumors, the majority of which were well-differentiated, were observed with variable latency, on average at 7-8 months of age in multiparious females. Fibroadenomas composed of neoplastic glandular alveolar epithelium surrounded by variable amounts of stroma were also observed. Metastasis to the lung was observed in 1 of 40 tumor-bearing mice. Virgin mice were tumor-free.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Wap     (12 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Tg(WapTgfa)215Bri/0

        FVB.Cg-Tg(WapTgfa)215Bri
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland morphology
    • apoptosis in the mammary gland are reduced prior to 12 weeks of age and increased after 16 weeks of age compared to in wild-type mice   (MGI Ref ID J:114568)
    • abnormal mammary gland lobule morphology
      • lobules contain more cells than in wild-type mice   (MGI Ref ID J:114568)
    • mammary gland duct hyperplasia
      • mice exhibit increased ductal cell proliferation with greater lobular development compared to in wild-type mice   (MGI Ref ID J:114568)
    • mammary gland hyperplasia
      • cell proliferation in lobules of the mammary gland is 7-fold higher than in wild-type mice after 16 weeks of age   (MGI Ref ID J:114568)
      • unlike in wild-type mice, proliferation rates remain high as mice age   (MGI Ref ID J:114568)
      • mice exhibit increased number and size of hyperplastic areas compared to in wild-type mice   (MGI Ref ID J:114568)
      • cell proliferation of normal cells in tumor containing glands is increased compared to in non-tumor containing glands   (MGI Ref ID J:114568)
  • tumorigenesis
  • increased mammary gland tumor incidence
    • tumors exhibit high cell proliferation rates similar to hyperpastic mammary gland tissue   (MGI Ref ID J:114568)
  • integument phenotype
  • abnormal mammary gland morphology
    • apoptosis in the mammary gland are reduced prior to 12 weeks of age and increased after 16 weeks of age compared to in wild-type mice   (MGI Ref ID J:114568)
    • abnormal mammary gland lobule morphology
      • lobules contain more cells than in wild-type mice   (MGI Ref ID J:114568)
    • mammary gland duct hyperplasia
      • mice exhibit increased ductal cell proliferation with greater lobular development compared to in wild-type mice   (MGI Ref ID J:114568)
    • mammary gland hyperplasia
      • cell proliferation in lobules of the mammary gland is 7-fold higher than in wild-type mice after 16 weeks of age   (MGI Ref ID J:114568)
      • unlike in wild-type mice, proliferation rates remain high as mice age   (MGI Ref ID J:114568)
      • mice exhibit increased number and size of hyperplastic areas compared to in wild-type mice   (MGI Ref ID J:114568)
      • cell proliferation of normal cells in tumor containing glands is increased compared to in non-tumor containing glands   (MGI Ref ID J:114568)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(WapTgfa)215Bri/0

        involves: C57BL/6 * SJL
  • mortality/aging
  • premature death
    • female mice die by 14 months of age   (MGI Ref ID J:56318)
  • endocrine/exocrine gland phenotype
  • abnormal involution of the mammary gland
  • abnormal lactation
    • female mice do not lactate   (MGI Ref ID J:56318)
  • abnormal mammary gland growth during pregnancy
    • female mice produce precocious small alveoli by day 14 of pregnancy unlike in wild-type mice   (MGI Ref ID J:56318)
  • mammary gland hyperplasia
    • female mice exhibit precocious glandular development   (MGI Ref ID J:56318)
    • glandular hyperplasia worsens with each pregnancy resulting in the creation of numerous hyperplastic alveolar nodules resembling tumors   (MGI Ref ID J:56318)
  • tumorigenesis
  • increased mammary gland tumor incidence
    • female mice develop mammary gland tumors and die by 14 months of age   (MGI Ref ID J:56318)
    • female mice develop well differentiated microcystic/glandular adenomas or carcinomas and papillary adenocarcinomas that resemble normal pregnant and lactating mammary epitherlium as well as fibroadenomas and occasionally solid carcinomas that do not resemble a normal mammary gland   (MGI Ref ID J:56318)
    • 17% of tumors are glandular/microcystic foci combined with papillary, fibroadenomatous or solid foci   (MGI Ref ID J:56318)
    • while lung metastasis is rare (in 1 of 40 mice), most tumors are transplantable   (MGI Ref ID J:56318)
    • however, virgin females do not develop mammary gland tumors   (MGI Ref ID J:56318)
    • mammary adenocarcinoma
      • most tumors are well differentiated adenomas and adenocarcinomas   (MGI Ref ID J:56318)
  • reproductive system phenotype
  • abnormal mammary gland growth during pregnancy
    • female mice produce precocious small alveoli by day 14 of pregnancy unlike in wild-type mice   (MGI Ref ID J:56318)
  • integument phenotype
  • abnormal involution of the mammary gland
  • abnormal lactation
    • female mice do not lactate   (MGI Ref ID J:56318)
  • abnormal mammary gland growth during pregnancy
    • female mice produce precocious small alveoli by day 14 of pregnancy unlike in wild-type mice   (MGI Ref ID J:56318)
  • mammary gland hyperplasia
    • female mice exhibit precocious glandular development   (MGI Ref ID J:56318)
    • glandular hyperplasia worsens with each pregnancy resulting in the creation of numerous hyperplastic alveolar nodules resembling tumors   (MGI Ref ID J:56318)

Tg(WapTgfa)215Bri/0

        B6.Cg-Tg(WapTgfa)215Bri
  • tumorigenesis
  • increased lymphoma incidence
    • mice exhibit pulmonary foci that display perivascular lymphocytic infiltration and perivascular lymphomas of unknown origins as well as 1 metastatic adenocarcinoma   (MGI Ref ID J:123146)
  • increased mammary gland tumor incidence
    • all mice develop mammary gland tumors within 1 year with mean onset at 6 months of age and earliest onset at 12 weeks of age   (MGI Ref ID J:123146)
  • endocrine/exocrine gland phenotype
  • mammary gland hyperplasia   (MGI Ref ID J:123146)
  • integument phenotype
  • mammary gland hyperplasia   (MGI Ref ID J:123146)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence
      Mammary Gland Tumors

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Tg(WapTgfa)215Bri
Allele Name transgene insertion 215, Ralph L Brinster
Allele Type Transgenic (random, expressed)
Mutation Made ByDr. Ralph Brinster,   University of Pennsylvania
Strain of Origin(C57BL/6 x SJL)F2
Expressed Gene Tgfa, transforming growth factor alpha, rat
Promoter Wap, whey acidic protein, mouse, laboratory
Molecular Note The transgene contains the mouse whey acidic protein (WAP) promoter and the mouse transforming growth factor alpha (Tgfa) gene. [MGI Ref ID J:56318]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(WapTgfa)215Bri, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Sandgren EP; Schroeder JA; Qui TH; Palmiter RD; Brinster RL; Lee DC. 1995. Inhibition of mammary gland involution is associated with transforming growth factor alpha but not c-myc-induced tumorigenesis in transgenic mice. Cancer Res 55(17):3915-27. [PubMed: 7641211]  [MGI Ref ID J:56318]

Additional References

Tg(WapTgfa)215Bri related

Pochampalli MR; Bitler BG; Schroeder JA. 2007. Transforming growth factor alpha dependent cancer progression is modulated by Muc1. Cancer Res 67(14):6591-8. [PubMed: 17638868]  [MGI Ref ID J:123146]

Rose-Hellekant TA; Wentworth KM; Nikolai S; Kundel DW; Sandgren EP. 2006. Mammary Carcinogenesis Is Preceded by Altered Epithelial Cell Turnover in Transforming Growth Factor-{alpha} and c-myc Transgenic Mice. Am J Pathol 169(5):1821-32. [PubMed: 17071603]  [MGI Ref ID J:114568]

Seitzer N; Mayr T; Streit S; Ullrich A. 2010. A single nucleotide change in the mouse genome accelerates breast cancer progression. Cancer Res 70(2):802-12. [PubMed: 20068154]  [MGI Ref ID J:156750]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3000.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

General Supply Notes

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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