Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names FVB/N-Tg(WapTgfa)215Bri/J    (Changed: 08-AUG-08 ) Type Congenic; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Generation N12   Donating Investigator Eric Sandgren,   Univ of Wisconsin School of Vet Med

Description
Transgenic females showed precocious mammary gland development during pregnancy. Involution was delayed beyond 8 days post-parturition. With successive pregnancies hyperplastic alveolar nodules increased in number. Mammary tumors, the majority of which were well-differentiated, were observed with variable latency, on average at 7-8 months of age in multiparious females. Fibroadenomas composed of neoplastic glandular alveolar epithelium surrounded by variable amounts of stroma were also observed. Metastasis to the lung was observed in 1 of 40 tumor-bearing mice. Virgin mice were tumor-free.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of Wap

003552	B6129-Tg(Wap-cre)11738Mam/J
002409	B6;SJL-Tg(Wap-HRAS)69Lln Chr YSJL/J
003189	C57BL/6J-Tg(WAPTAg)3Knw/J
002500	C57BL/6J-Tg(WapIgf1)39Dlr/J
003188	C57BL/6J-Tg(WapTAg)1Knw
002410	FVB.Cg-Tg(Wap-HRAS)69Lln Chr YSJL/J
003078	FVB.Cg-Tg(WapIgf1)39Dlr/J
002755	FVB/N-Tg(WapNotch4)10Rnc/J

View Strains carrying other alleles of Wap     (8 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(WapTgfa)215Bri/0

        FVB.Cg-Tg(WapTgfa)215Bri

• endocrine/exocrine gland phenotype
• abnormal mammary gland morphology (MGI Ref ID J:114568)
  • apoptosis in the mammary gland are reduced prior to 12 weeks of age and increased after 16 weeks of age compared to in wild-type mice
  • lobules contain more cells than in wild-type mice
• mammary ductal hyperplasia (MGI Ref ID J:114568)
  • mice exhibit increased ductal cell proliferation with greater lobular development compared to in wild-type mice
• mammary gland hyperplasia (MGI Ref ID J:114568)
  • cell proliferation in lobules of the mammary gland is 7-fold higher than in wild-type mice after 16 weeks of age
  • unlike in wild-type mice, proliferation rates remain high as mice age
  • mice exhibit increased number and size of hyperplastic areas compared to in wild-type mice
  • cell proliferation of normal cells in tumor containing glands is increased compared to in non-tumor containing glands
• tumorigenesis
• mammary gland tumor (MGI Ref ID J:114568)
  • tumors exhibit high cell proliferation rates similar to hyperpastic mammary gland tissue
• reproductive system phenotype
• abnormal mammary gland morphology (MGI Ref ID J:114568)
  • apoptosis in the mammary gland are reduced prior to 12 weeks of age and increased after 16 weeks of age compared to in wild-type mice
  • lobules contain more cells than in wild-type mice
• mammary ductal hyperplasia (MGI Ref ID J:114568)
  • mice exhibit increased ductal cell proliferation with greater lobular development compared to in wild-type mice
• mammary gland hyperplasia (MGI Ref ID J:114568)
  • cell proliferation in lobules of the mammary gland is 7-fold higher than in wild-type mice after 16 weeks of age
  • unlike in wild-type mice, proliferation rates remain high as mice age
  • mice exhibit increased number and size of hyperplastic areas compared to in wild-type mice
  • cell proliferation of normal cells in tumor containing glands is increased compared to in non-tumor containing glands

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(WapTgfa)215Bri/0

        involves: C57BL/6 * SJL

• life span-post-weaning/aging
• premature death (MGI Ref ID J:56318)
  • female mice die by 14 months of age
• endocrine/exocrine gland phenotype
• abnormal lactation (MGI Ref ID J:56318)
  • female mice do not lactate
• abnormal mammary gland morphology (MGI Ref ID J:56318)
  • female mice produce precocious small alveoli by day 14 of pregnancy unlike in wild-type mice
• abnormal involution of the mammary gland (MGI Ref ID J:56318)
  • delayed
• mammary gland hyperplasia (MGI Ref ID J:56318)
  • female mice exhibit precocious glandular development
  • glandular hyperplasia worsens with each pregnancy resulting in the creation of numerous hyperplastic alveolar nodules resembling tumors
• tumorigenesis
• mammary gland tumor (MGI Ref ID J:56318)
  • female mice develop mammary gland tumors and die by 14 months of age
  • female mice develop well differentiated microcystic/glandular adenomas or carcinomas and papillary adenocarcinomas that resemble normal pregnant and lactating mammary epitherlium as well as fibroadenomas and occasionally solid carcinomas that do not resemble a normal mammary gland
  • 17% of tumors are glandular/microcystic foci combined with papillary, fibroadenomatous or solid foci
  • while lung metastasis is rare (in 1 of 40 mice), most tumors are transplantable
  • however, virgin females do not develop mammary gland tumors
• mammary adenocarcinoma (MGI Ref ID J:56318)
  • most tumors are well differentiated adenomas and adenocarcinomas
• reproductive system phenotype
• abnormal lactation (MGI Ref ID J:56318)
  • female mice do not lactate
• abnormal mammary gland morphology (MGI Ref ID J:56318)
  • female mice produce precocious small alveoli by day 14 of pregnancy unlike in wild-type mice
• abnormal involution of the mammary gland (MGI Ref ID J:56318)
  • delayed
• mammary gland hyperplasia (MGI Ref ID J:56318)
  • female mice exhibit precocious glandular development
  • glandular hyperplasia worsens with each pregnancy resulting in the creation of numerous hyperplastic alveolar nodules resembling tumors

Tg(WapTgfa)215Bri/0

        B6.Cg-Tg(WapTgfa)215Bri

• tumorigenesis
• lymphoma (MGI Ref ID J:123146)
  • mice exhibit pulmonary foci that display perivascular lymphocytic infiltration and perivascular lymphomas of unknown origins as well as 1 metastatic adenocarcinoma
• mammary gland tumor (MGI Ref ID J:123146)
  • all mice develop mammary gland tumors within 1 year with mean onset at 6 months of age and earliest onset at 12 weeks of age
• endocrine/exocrine gland phenotype
• mammary gland hyperplasia (MGI Ref ID J:123146)
• reproductive system phenotype
• mammary gland hyperplasia (MGI Ref ID J:123146)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Mammary Gland Tumors)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(WapTgfa)215Bri
Allele Name		transgene insertion 215, Ralph L Brinster
Allele Type		Transgenic (random, expressed)
Mutation Made By		Ralph Brinster,   University of Pennsylvania
Strain of Origin		(C57BL/6 x SJL)F2
Expressed Gene		Tgfa, transforming growth factor alpha, rat
Promoter		Wap, whey acidic protein, mouse, laboratory
Molecular Note		The transgene contains the mouse whey acidic protein (WAP) promoter and the mouse transforming growth factor alpha (Tgfa) gene. [MGI Ref ID J:56318]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(WapTgfa)215Bri, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Sandgren EP; Schroeder JA; Qui TH; Palmiter RD; Brinster RL; Lee DC. 1995. Inhibition of mammary gland involution is associated with transforming growth factor alpha but not c-myc-induced tumorigenesis in transgenic mice. Cancer Res 55(17):3915-27. [PubMed: 7641211]  [MGI Ref ID J:56318]

Additional References

Tg(WapTgfa)215Bri related

Pochampalli MR; Bitler BG; Schroeder JA. 2007. Transforming growth factor alpha dependent cancer progression is modulated by Muc1. Cancer Res 67(14):6591-8. [PubMed: 17638868]  [MGI Ref ID J:123146]

Rose-Hellekant TA; Wentworth KM; Nikolai S; Kundel DW; Sandgren EP. 2006. Mammary Carcinogenesis Is Preceded by Altered Epithelial Cell Turnover in Transforming Growth Factor-{alpha} and c-myc Transgenic Mice. Am J Pathol 169(5):1821-32. [PubMed: 17071603]  [MGI Ref ID J:114568]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
	001800 FVB/NJ
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

OncoMouse^® requires a license from DuPont, see Licenses for Strains with OncoMouse^® Technology.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.