Strain Name:

B6.129P2-Ccl3tm1Unc/J

Stock Number:

002687

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Mice homozygous for the Ccl3 (chemokine [C-C motif] ligand 3) (also known as macrophage inflammatory protein 1a, Mip1a or Scya3) knock-out mutation show multiple deficiencies including clearance of influenza virus, bone remodeling during orthodontic tooth movement, liver fibrosis after treatments to induce fibrosis, metastasis with melanoma (but not with renal cell carcinoma where intra-tumoral neovascularization is attenuated). These mice can be useful in studies of chemokine receptors in inflammatory diseases.

Description

Strain Information

Former Names B6.129P2-Scya3tm1Unc    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129P2 via E14TG2a ES cell line
Generation[N7F16p]N1N1F10 (18-DEC-13)
Generation Definitions
 
Donating InvestigatorDr. Oliver Smithies,   University of North Carolina

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Ccl3 (chemokine [C-C motif] ligand 3) (also known as macrophage inflammatory protein 1a, Mip1a or Scya3) knock-out mutation are viable and fertile. Homozygous mutant mice are resistant to Coxsakievirus-induced myocarditis. Mutant mice infected with influenza virus show reduced pneumonitis and delayed clearance of virus. There are no overt hematopoietic abnormalities. Bone remodeling induced by mechanical loading during orthodontic tooth movement was significantly decreased in Ccl3tm1Unc mice. Compared to wild-type mice liver fibrosis was reduced upon treatment with either carbon tetrachloride or methionine- and choline-deficient diets to induce fibrosis. Melanoma growth is augmented and lung metastasis is enhanced in these knock-out mice. With renal cell carcinoma, however, the number of metastasis foci in the lung is reduced, and intra-tumoral neovascularization, an indispensable process for metastasis, is attenuated in these gene-deficient mice. These mice can be useful in studies of chemokine receptors in inflammatory diseases.

Development
A neomycin selection cassette was used to delete a region including 300 nucleotides of the 5' un-translated region, all of exon 1, and a portion of exon 2. Correctly targeted 129 ES cells were injected into C57BL/6J blastocysts.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Human Immunodeficiency Virus Type 1, Susceptibility to   (CCL3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Ccl3tm1Unc/Ccl3tm1Unc

        B6.129P2-Ccl3tm1Unc/J
  • behavior/neurological phenotype
  • abnormal conditioned taste aversion behavior
    • male mice consume more saccharin in an ethanol-induced conditioned taste aversion test compared with similarly treated wild-type mice   (MGI Ref ID J:102583)
    • mice exhibit a stronger response to ethanol in an ethanol-induced conditioned taste aversion test compared with similarly treated wild-type mice   (MGI Ref ID J:102583)
    • however, saccharin consumption in female mice is normal   (MGI Ref ID J:102583)
  • alcohol aversion   (MGI Ref ID J:102583)
  • decreased alcohol consumption   (MGI Ref ID J:102583)
  • impaired righting response
    • mice exhibit longer duration of ethanol-induced loss of righting response compared with similarly treated wild-type mice   (MGI Ref ID J:102583)
  • increased fluid intake
    • male mice exhibit increased drinking of an ethanol or quinine solution compared with similarly treated wild-type mice   (MGI Ref ID J:102583)
  • immune system phenotype
  • decreased neutrophil cell number
    • 13-28% decrease in the number of infiltrating neutrophils in the skin at 4 and 8 hours after immune complex challenge compared to wild-type   (MGI Ref ID J:94599)
  • decreased susceptibility to type III hypersensitivity reaction
    • 20% reduction in edema, but no difference in hemorrhage, when cutaneous reverse passive Arthus reaction was induced compared to wild-type   (MGI Ref ID J:94599)
  • hematopoietic system phenotype
  • decreased neutrophil cell number
    • 13-28% decrease in the number of infiltrating neutrophils in the skin at 4 and 8 hours after immune complex challenge compared to wild-type   (MGI Ref ID J:94599)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Ccl3tm1Unc/Ccl3tm1Unc

        involves: 129P2/OlaHsd
  • immune system phenotype
  • cecum inflammation
    • mice treated with toxin A exhibit less severe enteritis (reduced fluid accumulation, neutrophil infiltration, and epithelial damage) compared with wild-type mice   (MGI Ref ID J:107677)
  • decreased susceptibility to bacterial infection
    • mice treated with toxin A exhibit less severe enteritis (reduced fluid accumulation, neutrophil infiltration, and epithelial damage) compared with wild-type mice   (MGI Ref ID J:107677)
  • decreased susceptibility to viral infection
    • resistance to Coxsackie virus-induced myocarditis and reduced pneumonitis after infection with influenza virus   (MGI Ref ID J:28704)
    • influenza viral titers are higher in mutants at day 3, 6 and 7 post infection compared to wild-type, however by day 21 post infection, no virus is detected, indicating a delay in T cell-dependent viral clearance   (MGI Ref ID J:28704)
  • digestive/alimentary phenotype
  • cecum inflammation
    • mice treated with toxin A exhibit less severe enteritis (reduced fluid accumulation, neutrophil infiltration, and epithelial damage) compared with wild-type mice   (MGI Ref ID J:107677)

Ccl3tm1Unc/Ccl3tm1Unc

        involves: 129P2/OlaHsd * C57BL/6
  • homeostasis/metabolism phenotype
  • *normal* homeostasis/metabolism phenotype
    • mice exhibit normal wound healing   (MGI Ref ID J:70862)
  • nervous system phenotype
  • abnormal astrocyte physiology
    • significant reduction in activated astrocytes in the hippocampus 6 hours after injection of beta amyloid 1-40   (MGI Ref ID J:153059)
  • abnormal microglial cell activation
    • significant reduction in activated microglia in the hippocampus 8 hours after injection of beta amyloid 1-40   (MGI Ref ID J:153059)
  • behavior/neurological phenotype
  • abnormal long term spatial reference memory
    • in a Morris water maze   (MGI Ref ID J:153059)
  • abnormal spatial learning
    • improved learning performance in a Morris water maze after injection of beta amyloid 1-40 relative to controls   (MGI Ref ID J:153059)
  • immune system phenotype
  • abnormal microglial cell activation
    • significant reduction in activated microglia in the hippocampus 8 hours after injection of beta amyloid 1-40   (MGI Ref ID J:153059)
  • hematopoietic system phenotype
  • abnormal microglial cell activation
    • significant reduction in activated microglia in the hippocampus 8 hours after injection of beta amyloid 1-40   (MGI Ref ID J:153059)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Ccl3tm1Unc related

Cancer Research
Growth Factors/Receptors/Cytokines

Immunology, Inflammation and Autoimmunity Research
Growth Factors/Receptors/Cytokines
Inflammation

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Ccl3tm1Unc
Allele Name targeted mutation 1, University of North Carolina
Allele Type Targeted (Null/Knockout)
Common Name(s) MIP-1alpha -; Scya3 -; Scya3tm1Coo;
Mutation Made ByDr. Oliver Smithies,   University of North Carolina
Strain of Origin129P2/OlaHsd
ES Cell Line NameE14TG2a
ES Cell Line Strain129P2/OlaHsd
Gene Symbol and Name Ccl3, chemokine (C-C motif) ligand 3
Chromosome 11
Gene Common Name(s) 464.2; AI323804; D17S1718; G0S19-1; G0S19-2; LD78; LD78BETA; LD78alpha; MIP-1 alpha; MIP-1a; MIP-1alpha; MIP1-(a); MIP1-alpha; Mip1a; SCYA3L; SCYA3L1; Scya3; expressed sequence AI323804; macrophage inflammatory protein-1 alpha; macrophage inflammatory protein-1alpha; small inducible cytokine A3;
Molecular Note A neomycin selection cassette was used to delete a region including 300 nucleotides of the 5' untranslated region, all of exon 1, and a portion of exon 2. An absence of transcript was observed via Northern blot analysis of total mRNA derived from bone marrow macrophages isolated from homozygous mutant animals. [MGI Ref ID J:28704]

Genotyping

Genotyping Information

Genotyping Protocols

Ccl3tm1Unc, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Cook DN; Beck MA; Coffman TM; Kirby SL; Sheridan JF; Pragnell IB; Smithies O. 1995. Requirement of MIP-1 alpha for an inflammatory response to viral infection. Science 269(5230):1583-5. [PubMed: 7667639]  [MGI Ref ID J:28704]

Additional References

Salazar-Mather TP; Lewis CA; Biron CA. 2002. Type I interferons regulate inflammatory cell trafficking and macrophage inflammatory protein 1alpha delivery to the liver. J Clin Invest 110(3):321-30. [PubMed: 12163451]  [MGI Ref ID J:78321]

Wu YP; Proia RL. 2004. Deletion of macrophage-inflammatory protein 1 alpha retards neurodegeneration in Sandhoff disease mice. Proc Natl Acad Sci U S A 101(22):8425-30. [PubMed: 15155903]  [MGI Ref ID J:90687]

Ccl3tm1Unc related

Ajuebor MN; Hogaboam CM; Le T; Proudfoot AE; Swain MG. 2004. CCL3/MIP-1alpha is pro-inflammatory in murine T cell-mediated hepatitis by recruiting CCR1-expressing CD4(+) T cells to the liver. Eur J Immunol 34(10):2907. [PubMed: 15368307]  [MGI Ref ID J:92429]

Baba T; Naka K; Morishita S; Komatsu N; Hirao A; Mukaida N. 2013. MIP-1alpha/CCL3-mediated maintenance of leukemia-initiating cells in the initiation process of chronic myeloid leukemia. J Exp Med 210(12):2661-73. [PubMed: 24166712]  [MGI Ref ID J:207721]

Bignon A; Gaudin F; Hemon P; Tharinger H; Mayol K; Walzer T; Loetscher P; Peuchmaur M; Berrebi D; Balabanian K. 2014. CCR1 inhibition ameliorates the progression of lupus nephritis in NZB/W mice. J Immunol 192(3):886-96. [PubMed: 24367031]  [MGI Ref ID J:207311]

Blednov YA; Bergeson SE; Walker D; Ferreira VM; Kuziel WA; Harris RA. 2005. Perturbation of chemokine networks by gene deletion alters the reinforcing actions of ethanol. Behav Brain Res 165(1):110-25. [PubMed: 16105698]  [MGI Ref ID J:102583]

Brito BE; O'Rourke LM; Pan Y; Huang X; Park JM; Zamora D; Cook DN; Planck SR; Rosenbaum JT. 1999. Murine endotoxin-induced uveitis, but not immune complex-induced uveitis, is dependent on the IL-8 receptor homolog. Curr Eye Res 19(1):76-85. [PubMed: 10415460]  [MGI Ref ID J:56314]

Cameron MJ; Arreaza GA; Grattan M; Meagher C; Sharif S; Burdick MD; Strieter RM; Cook DN; Delovitch TL. 2000. Differential expression of CC chemokines and the CCR5 receptor in the pancreas is associated with progression to type I diabetes. J Immunol 165(2):1102-10. [PubMed: 10878389]  [MGI Ref ID J:120456]

Castor MG; Rezende B; Resende CB; Alessandri AL; Fagundes CT; Sousa LP; Arantes RM; Souza DG; Silva TA; Proudfoot AE; Teixeira MM; Pinho V. 2010. The CCL3/macrophage inflammatory protein-1alpha-binding protein evasin-1 protects from graft-versus-host disease but does not modify graft-versus-leukemia in mice. J Immunol 184(5):2646-54. [PubMed: 20100934]  [MGI Ref ID J:159661]

Chantakru S; Kuziel WA; Maeda N; Croy BA. 2001. A study on the density and distribution of uterine natural killer cells at mid pregnancy in mice genetically-ablated for CCR2, CCR 5 and the CCR5 receptor ligand, MIP-1alpha J Reprod Immunol 49(1):33-47. [PubMed: 11137111]  [MGI Ref ID J:66438]

Charmoy M; Brunner-Agten S; Aebischer D; Auderset F; Launois P; Milon G; Proudfoot AE; Tacchini-Cottier F. 2010. Neutrophil-derived CCL3 is essential for the rapid recruitment of dendritic cells to the site of Leishmania major inoculation in resistant mice. PLoS Pathog 6(2):e1000755. [PubMed: 20140197]  [MGI Ref ID J:162176]

Craig VJ; Quintero PA; Fyfe SE; Patel AS; Knolle MD; Kobzik L; Owen CA. 2013. Profibrotic Activities for Matrix Metalloproteinase-8 during Bleomycin-Mediated Lung Injury. J Immunol 190(8):4283-96. [PubMed: 23487425]  [MGI Ref ID J:195119]

Crow AR; Song S; Semple JW; Freedman J; Lazarus AH. 2007. A role for IL-1 receptor antagonist or other cytokines in the acute therapeutic effects of IVIg? Blood 109(1):155-8. [PubMed: 16954498]  [MGI Ref ID J:142178]

Delogu A; Schebesta A; Sun Q; Aschenbrenner K; Perlot T; Busslinger M. 2006. Gene repression by Pax5 in B cells is essential for blood cell homeostasis and is reversed in plasma cells. Immunity 24(3):269-81. [PubMed: 16546096]  [MGI Ref ID J:113322]

Domachowske JB; Bonville CA; Gao JL; Murphy PM; Easton AJ; Rosenberg HF. 2000. The chemokine macrophage-inflammatory protein-1 alpha and its receptor CCR1 control pulmonary inflammation and antiviral host defense in paramyxovirus infection. J Immunol 165(5):2677-82. [PubMed: 10946298]  [MGI Ref ID J:120155]

Hamrah P; Yamagami S; Liu Y; Zhang Q; Vora SS; Lu B; Gerard CJ; Dana MR. 2007. Deletion of the chemokine receptor CCR1 prolongs corneal allograft survival. Invest Ophthalmol Vis Sci 48(3):1228-36. [PubMed: 17325167]  [MGI Ref ID J:123260]

Heinrichs D; Berres ML; Nellen A; Fischer P; Scholten D; Trautwein C; Wasmuth HE; Sahin H. 2013. The chemokine CCL3 promotes experimental liver fibrosis in mice. PLoS One 8(6):e66106. [PubMed: 23799074]  [MGI Ref ID J:203470]

Hsieh CH; Frink M; Hsieh YC; Kan WH; Hsu JT; Schwacha MG; Choudhry MA; Chaudry IH. 2008. The role of MIP-1 alpha in the development of systemic inflammatory response and organ injury following trauma hemorrhage. J Immunol 181(4):2806-12. [PubMed: 18684972]  [MGI Ref ID J:140173]

Ishida Y; Kimura A; Kondo T; Hayashi T; Ueno M; Takakura N; Matsushima K; Mukaida N. 2007. Essential Roles of the CC Chemokine Ligand 3-CC Chemokine Receptor 5 Axis in Bleomycin-Induced Pulmonary Fibrosis through Regulation of Macrophage and Fibrocyte Infiltration. Am J Pathol 170(3):843-54. [PubMed: 17322370]  [MGI Ref ID J:118647]

Kang SJ; Liang HE; Reizis B; Locksley RM. 2008. Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells. Immunity 29(5):819-33. [PubMed: 19006696]  [MGI Ref ID J:142395]

Kim JH; Chung DH. 2011. CD1d-restricted IFN-gamma-secreting NKT cells promote immune complex-induced acute lung injury by regulating macrophage-inflammatory protein-1alpha production and activation of macrophages and dendritic cells. J Immunol 186(3):1432-41. [PubMed: 21191075]  [MGI Ref ID J:168903]

Kohno H; Maeda T; Perusek L; Pearlman E; Maeda A. 2014. CCL3 production by microglial cells modulates disease severity in murine models of retinal degeneration. J Immunol 192(8):3816-27. [PubMed: 24639355]  [MGI Ref ID J:210001]

Lassen MG; Lukens JR; Dolina JS; Brown MG; Hahn YS. 2010. Intrahepatic IL-10 maintains NKG2A+Ly49- liver NK cells in a functionally hyporesponsive state. J Immunol 184(5):2693-701. [PubMed: 20124099]  [MGI Ref ID J:159651]

Lindell DM; Standiford TJ; Mancuso P; Leshen ZJ; Huffnagle GB. 2001. Macrophage Inflammatory Protein 1alpha/CCL3 Is Required for Clearance of an Acute Klebsiella pneumoniae Pulmonary Infection. Infect Immun 69(10):6364-9. [PubMed: 11553580]  [MGI Ref ID J:71659]

Lopez ME; Klein AD; Hong J; Dimbil UJ; Scott MP. 2012. Neuronal and epithelial cell rescue resolves chronic systemic inflammation in the lipid storage disorder Niemann-Pick C. Hum Mol Genet 21(13):2946-60. [PubMed: 22493001]  [MGI Ref ID J:184610]

Low QE; Drugea IA; Duffner LA; Quinn DG; Cook DN; Rollins BJ; Kovacs EJ; DiPietro LA. 2001. Wound healing in MIP-1alpha(-/-) and MCP-1(-/-) mice. Am J Pathol 159(2):457-63. [PubMed: 11485904]  [MGI Ref ID J:70862]

Lu P; Li L; Wu Y; Mukaida N; Zhang X. 2008. Essential contribution of CCL3 to alkali-induced corneal neovascularization by regulating vascular endothelial growth factor production by macrophages. Mol Vis 14:1614-22. [PubMed: 18776949]  [MGI Ref ID J:140117]

Mahesh J; Daly J; Cheadle WG; Kotwal GJ. 1999. Elucidation of the early events contributing to zymosan-induced multiple organ dysfunction syndrome using MIP-1alpha, C3 knockout, and C5-deficient mice. Shock 12(5):340-9. [PubMed: 10565608]  [MGI Ref ID J:59655]

McMahon EJ; Cook DN; Suzuki K; Matsushima GK. 2001. Absence of macrophage-inflammatory protein-1alpha delays central nervous system demyelination in the presence of an intact blood-brain barrier. J Immunol 167(5):2964-71. [PubMed: 11509646]  [MGI Ref ID J:118563]

Miller CG; Cook DN; Kotwal GJ. 1996. Two chemotactic factors, C5a and MIP-1alpha, dramatically alter the mortality from zymosan-induced multiple organ dysfunction syndrome (MODS): C5a contributes to MODS while MIP-1alpha has a protective role. Mol Immunol 33(14):1135-7. [PubMed: 9047380]  [MGI Ref ID J:38592]

Miyazaki D; Nakamura T; Toda M; Cheung-Chau KW; Richardson RM; Ono SJ. 2005. Macrophage inflammatory protein-1alpha as a costimulatory signal for mast cell-mediated immediate hypersensitivity reactions. J Clin Invest 115(2):434-42. [PubMed: 15650768]  [MGI Ref ID J:96185]

Morteau O; Castagliuolo I; Mykoniatis A; Zacks J; Wlk M; Lu B; Pothoulakis C; Gerard NP; Gerard C. 2002. Genetic deficiency in the chemokine receptor CCR1 protects against acute Clostridium difficile toxin A enteritis in mice. Gastroenterology 122(3):725-33. [PubMed: 11875005]  [MGI Ref ID J:107677]

Nakasone Y; Fujimoto M; Matsushita T; Hamaguchi Y; Huu DL; Yanaba M; Sato S; Takehara K; Hasegawa M. 2012. Host-derived MCP-1 and MIP-1alpha regulate protective anti-tumor immunity to localized and metastatic B16 melanoma. Am J Pathol 180(1):365-74. [PubMed: 22037251]  [MGI Ref ID J:180235]

Narni-Mancinelli E; Campisi L; Bassand D; Cazareth J; Gounon P; Glaichenhaus N; Lauvau G. 2007. Memory CD8+ T cells mediate antibacterial immunity via CCL3 activation of TNF/ROI+ phagocytes. J Exp Med 204(9):2075-87. [PubMed: 17698589]  [MGI Ref ID J:126089]

Oldford SA; Haidl ID; Howatt MA; Leiva CA; Johnston B; Marshall JS. 2010. A Critical Role for Mast Cells and Mast Cell-Derived IL-6 in TLR2-Mediated Inhibition of Tumor Growth. J Immunol 185(11):7067-76. [PubMed: 21041732]  [MGI Ref ID J:166133]

Olszewski MA; Huffnagle GB; McDonald RA; Lindell DM; Moore BB; Cook DN; Toews GB. 2000. The role of macrophage inflammatory protein-1 alpha/CCL3 in regulation of T cell-mediated immunity to Cryptococcus neoformans infection. J Immunol 165(11):6429-36. [PubMed: 11086082]  [MGI Ref ID J:118399]

Olszewski MA; Huffnagle GB; Traynor TR; McDonald RA; Cook DN; Toews GB. 2001. Regulatory Effects of Macrophage Inflammatory Protein 1alpha/CCL3 on the Development of Immunity to Cryptococcus neoformans Depend on Expression of Early Inflammatory Cytokines. Infect Immun 69(10):6256-63. [PubMed: 11553568]  [MGI Ref ID J:71647]

Passos GF; Figueiredo CP; Prediger RD; Pandolfo P; Duarte FS; Medeiros R; Calixto JB. 2009. Role of the macrophage inflammatory protein-1alpha/CC chemokine receptor 5 signaling pathway in the neuroinflammatory response and cognitive deficits induced by beta-amyloid peptide. Am J Pathol 175(4):1586-97. [PubMed: 19729478]  [MGI Ref ID J:153059]

Quintero PA; Knolle MD; Cala LF; Zhuang Y; Owen CA. 2010. Matrix metalloproteinase-8 inactivates macrophage inflammatory protein-1 alpha to reduce acute lung inflammation and injury in mice. J Immunol 184(3):1575-88. [PubMed: 20042585]  [MGI Ref ID J:159502]

Ramos CD; Canetti C; Souto JT; Silva JS; Hogaboam CM; Ferreira SH; Cunha FQ. 2005. MIP-1{alpha}[CCL3] acting on the CCR1 receptor mediates neutrophil migration in immune inflammation via sequential release of TNF-{alpha} and LTB4. J Leukoc Biol 78(1):167-77. [PubMed: 15831559]  [MGI Ref ID J:99288]

Ramos CD; Fernandes KS; Canetti C; Teixeira MM; Silva JS; Cunha FQ. 2006. Neutrophil recruitment in immunized mice depends on MIP-2 inducing the sequential release of MIP-1alpha, TNF-alpha and LTB(4). Eur J Immunol 36(8):2025-34. [PubMed: 16856209]  [MGI Ref ID J:116027]

Repeke CE; Ferreira SB Jr; Claudino M; Silveira EM; de Assis GF; Avila-Campos MJ; Silva JS; Garlet GP. 2010. Evidences of the cooperative role of the chemokines CCL3, CCL4 and CCL5 and its receptors CCR1+ and CCR5+ in RANKL+ cell migration throughout experimental periodontitis in mice. Bone 46(4):1122-30. [PubMed: 20053385]  [MGI Ref ID J:162053]

Salazar-Mather TP; Hamilton TA; Biron CA. 2000. A chemokine-to-cytokine-to-chemokine cascade critical in antiviral defense. J Clin Invest 105(7):985-93. [PubMed: 10749577]  [MGI Ref ID J:120540]

Salem ML; Al-Khami AA; El-Naggar SA; Diaz-Montero CM; Chen Y; Cole DJ. 2010. Cyclophosphamide induces dynamic alterations in the host microenvironments resulting in a Flt3 ligand-dependent expansion of dendritic cells. J Immunol 184(4):1737-47. [PubMed: 20083664]  [MGI Ref ID J:159482]

Sato N; Ahuja SK; Quinones M; Kostecki V; Reddick RL; Melby PC; Kuziel WA; Ahuja SS. 2000. CC chemokine receptor (CCR)2 is required for langerhans cell migration and localization of T helper cell type 1 (Th1)-inducing dendritic cells. Absence of CCR2 shifts the Leishmania major-resistant phenotype to a susceptible state dominated by Th2 cytokines, b cell outgrowth, and sustained neutrophilic inflammation. J Exp Med 192(2):205-18. [PubMed: 10899907]  [MGI Ref ID J:63491]

Sato N; Kuziel WA; Melby PC; Reddick RL; Kostecki V; Zhao W; Maeda N; Ahuja SK; Ahuja SS. 1999. Defects in the generation of IFN-gamma are overcome to control infection with Leishmania donovani in CC chemokine receptor (CCR) 5-, macrophage inflammatory protein-1 alpha-, or CCR2-deficient mice. J Immunol 163(10):5519-25. [PubMed: 10553079]  [MGI Ref ID J:91256]

Serody JS; Burkett SE; Panoskaltsis-Mortari A; Ng-Cashin J; McMahon E; Matsushima GK; Lira SA; Cook DN; Blazar BR. 2000. T-lymphocyte production of macrophage inflammatory protein-1alpha is critical to the recruitment of CD8(+) T cells to the liver, lung, and spleen during graft-versus-host disease Blood 96(9):2973-80. [PubMed: 11049973]  [MGI Ref ID J:65501]

Serody JS; Cook DN; Kirby SL; Reap E; Shea TC; Frelinger JA. 1999. Murine T lymphocytes incapable of producing macrophage inhibitory protein-1 are impaired in causing graft-versus-host disease across a class I but not class II major histocompatibility complex barrier. Blood 93(1):43-50. [PubMed: 9864144]  [MGI Ref ID J:51842]

Souza AL; Roffe E; Pinho V; Souza DG; Silva AF; Russo RC; Guabiraba R; Pereira CA; Carvalho FM; Barsante MM; Correa-Oliveira R; Fraga LA; Negrao-Correa D; Teixeira MM. 2005. Potential role of the chemokine macrophage inflammatory protein 1alpha in human and experimental schistosomiasis. Infect Immun 73(4):2515-23. [PubMed: 15784598]  [MGI Ref ID J:97199]

Surmi BK; Webb CD; Ristau AC; Hasty AH. 2010. Absence of macrophage inflammatory protein-1{alpha} does not impact macrophage accumulation in adipose tissue of diet-induced obese mice. Am J Physiol Endocrinol Metab 299(3):E437-45. [PubMed: 20551286]  [MGI Ref ID J:170031]

Szretter KJ; Gangappa S; Lu X; Smith C; Shieh WJ; Zaki SR; Sambhara S; Tumpey TM; Katz JM. 2007. Role of host cytokine responses in the pathogenesis of avian H5N1 influenza viruses in mice. J Virol 81(6):2736-44. [PubMed: 17182684]  [MGI Ref ID J:153325]

Taddei SR; Queiroz CM Jr; Moura AP; Andrade I Jr; Garlet GP; Proudfoot AE; Teixeira MM; da Silva TA. 2013. The effect of CCL3 and CCR1 in bone remodeling induced by mechanical loading during orthodontic tooth movement in mice. Bone 52(1):259-67. [PubMed: 23059626]  [MGI Ref ID J:193779]

Trifilo MJ; Bergmann CC; Kuziel WA; Lane TE. 2003. CC chemokine ligand 3 (CCL3) regulates CD8(+)-T-cell effector function and migration following viral infection. J Virol 77(7):4004-14. [PubMed: 12634360]  [MGI Ref ID J:124745]

Van de Walle GR; Sakamoto K; Osterrieder N. 2008. CCL3 and viral chemokine-binding protein gg modulate pulmonary inflammation and virus replication during equine herpesvirus 1 infection. J Virol 82(4):1714-22. [PubMed: 18077722]  [MGI Ref ID J:141531]

Verri WA Jr; Cunha TM; Ferreira SH; Wei X; Leung BP; Fraser A; McInnes IB; Liew FY; Cunha FQ. 2007. IL-15 mediates antigen-induced neutrophil migration by triggering IL-18 production. Eur J Immunol 37(12):3373-80. [PubMed: 17979156]  [MGI Ref ID J:128427]

Wu Y; Li YY; Matsushima K; Baba T; Mukaida N. 2008. CCL3-CCR5 axis regulates intratumoral accumulation of leukocytes and fibroblasts and promotes angiogenesis in murine lung metastasis process. J Immunol 181(9):6384-93. [PubMed: 18941229]  [MGI Ref ID J:140725]

Wu YP; Proia RL. 2004. Deletion of macrophage-inflammatory protein 1 alpha retards neurodegeneration in Sandhoff disease mice. Proc Natl Acad Sci U S A 101(22):8425-30. [PubMed: 15155903]  [MGI Ref ID J:90687]

Yamagami S; Hamrah P; Miyamoto K; Miyazaki D; Dekaris I; Dawson T; Lu B; Gerard C; Dana MR. 2005. CCR5 chemokine receptor mediates recruitment of MHC class II-positive Langerhans cells in the mouse corneal epithelium. Invest Ophthalmol Vis Sci 46(4):1201-7. [PubMed: 15790880]  [MGI Ref ID J:104985]

Yanaba K; Mukaida N; Matsushima K; Murphy PM; Takehara K; Sato S. 2004. Role of C-C chemokine receptors 1 and 5 and CCL3/macrophage inflammatory protein-1alpha in the cutaneous Arthus reaction: possible attenuation of their inhibitory effects by compensatory chemokine production. Eur J Immunol 34(12):3553-61. [PubMed: 15517609]  [MGI Ref ID J:94599]

Yang X; Lu P; Fujii C; Nakamoto Y; Gao JL; Kaneko S; Murphy PM; Mukaida N. 2006. Essential contribution of a chemokine, CCL3, and its receptor, CCR1, to hepatocellular carcinoma progression. Int J Cancer 118(8):1869-76. [PubMed: 16284949]  [MGI Ref ID J:106825]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           MGL375

Colony Maintenance

Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Ccl3tm1Unc  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Ccl3tm1Unc x Homozygous for Ccl3tm1Unc  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Ccl3tm1Unc  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Ccl3tm1Unc x Homozygous for Ccl3tm1Unc  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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