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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names C57BL/6JEi-Tg(Sry)2Ei Chr YAKR/Ei (Changed: 16-MAY-07 ) C57BL/6JEi-Chr YAKR Tg(Sry)2Ei (Changed: 14-AUG-06 ) C57BL/6JEi-YAKR TgN(Sry)2Ei (Changed: 15-DEC-04 ) Type Consomic; Additional information on Consomic Mice. Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N6+ Donating Investigator Eva Eicher, The Jackson Laboratory Description
In this strain, XX mice that carry the Tg(Sry)2Ei transgene are sex-reversed males.Development
The 129-derived 14.6-kb of genomic sequence encoding Sry, described by Gubbay et al. (Nature, 1990. v346:245), was injected into the male pronuclei of fertilized eggs obtained from mating C57BL/6JEi females with C57BL/6JEi-Chr YAKR/J males. Three XY transgenic male founders were obtained and each generated a line that was maintained by backcrossing transgenic males, obligatorily carrying the consomic Chr YAKR/J, to C57BL/6JEi females (Eicher et al., 2001). All three strains were independently cryopreserved.
Related Strains
Other Related Strains
002063 C57BL/6JEi-Tg(Sry)1Ei Chr YAKR/J/EiJ 002837 C57BL/6JEi-Tg(Sry)3Ei Chr YAKR/J/EiJ View Other Related Strains (2 strains)
Phenotype
Phenotype Information
Currently there is no phenotype information for this strain.This mouse can be used to support research in many areas including:Tg(Sry)2Ei related
Developmental Biology Research
Internal/Organ Defects (gonads)
Reproductive Biology Research
Developmental Defects Affecting Gonads
Research Tools
Developmental Biology Research
Reproductive Biology Research
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Sry)2Ei | ||
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| Allele Name | transgene insertion 2, Eva M Eicher | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | Tg(Sry-129)2Ei; | ||
| Strain of Origin | C57BL/6JEi-Chr Y | ||
| Molecular Note | This transgene consists of an inbred 129 strain derived 14.6 Kb genomic fragment containing the entire Sry open reading frame and no other genes. [MGI Ref ID J:71077] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Additional References
Tg(Sry)2Ei relatedAlbrecht KH; Young M; Washburn LL; Eicher EM. 2003. Sry expression level and protein isoform differences play a role in abnormal testis development in C57BL/6J mice carrying certain Sry alleles. Genetics 164(1):277-88. [PubMed: 12750339] [MGI Ref ID J:127090]
Carruth LL; Reisert I; Arnold AP. 2002. Sex chromosome genes directly affect brain sexual differentiation. Nat Neurosci 5(10):933-4. [PubMed: 12244322] [MGI Ref ID J:130009]
Gatewood JD; Wills A; Shetty S; Xu J; Arnold AP; Burgoyne PS; Rissman EF. 2006. Sex chromosome complement and gonadal sex influence aggressive and parental behaviors in mice. J Neurosci 26(8):2335-42. [PubMed: 16495461] [MGI Ref ID J:105767]
Mahadevaiah SK; Odorisio T; Elliott DJ; Rattigan A; Szot M; Laval SH ; Washburn LL ; McCarrey JR ; Cattanach BM ; Lovell-Badge R ; Burgoyne PS. 1998. Mouse homologues of the human AZF candidate gene RBM are expressed in spermatogonia and spermatids, and map to a Y chromosome deletion interval associated with a high incidence of sperm abnormalities. Hum Mol Genet 7(4):715-27. [PubMed: 9499427] [MGI Ref ID J:46791]
Quinn JJ; Hitchcott PK; Umeda EA; Arnold AP; Taylor JR. 2007. Sex chromosome complement regulates habit formation. Nat Neurosci 10(11):1398-400. [PubMed: 17952068] [MGI Ref ID J:128462]
Smith-Bouvier DL; Divekar AA; Sasidhar M; Du S; Tiwari-Woodruff SK; King JK; Arnold AP; Singh RR; Voskuhl RR. 2008. A role for sex chromosome complement in the female bias in autoimmune disease. J Exp Med 205(5):1099-108. [PubMed: 18443225] [MGI Ref ID J:136240]
Szot M; Grigoriev V; Mahadevaiah SK; Ojarikre OA; Toure A; von Glasenapp E; Rattigan A; Turner JM; Elliott DJ; Burgoyne PS. 2003. Does Rbmy have a role in sperm development in mice? Cytogenet Genome Res 103(3-4):330-6. [PubMed: 15051956] [MGI Ref ID J:89312]
Toure A; Szot M; Mahadevaiah SK; Rattigan A; Ojarikre OA; Burgoyne PS. 2004. A new deletion of the mouse Y chromosome long arm associated with the loss of Ssty expression, abnormal sperm development and sterility. Genetics 166(2):901-12. [PubMed: 15020475] [MGI Ref ID J:89616]
Wagner CK; Xu J; Pfau JL; Quadros PS; De Vries GJ; Arnold AP. 2004. Neonatal mice possessing an Sry transgene show a masculinized pattern of progesterone receptor expression in the brain independent of sex chromosome status. Endocrinology 145(3):1046-9. [PubMed: 14645115] [MGI Ref ID J:88702]
Ward MA; Burgoyne PS. 2006. The Effects of Deletions of the Mouse Y Chromosome Long Arm on Sperm Function--Intracytoplasmic Sperm Injection (ICSI)-Based Analysis. Biol Reprod 74(4):652-8. [PubMed: 16354792] [MGI Ref ID J:107012]
Washburn LL; Albrecht KH; Eicher EM. 2001. C57BL/6J-T-Associated Sex Reversal in Mice Is Caused by Reduced Expression of a Mus domesticus Sry Allele. Genetics 158(4):1675-81. [PubMed: 11514455] [MGI Ref ID J:71077]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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