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Strain Name:

B6;C3-Tg(TettTALuc)1Dgs/J

Stock Number:

002709

Availability:

Repository- Live

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Additional Licensing and Use Restrictions

Genes & Alleles   luc;   tTA;   Tg(TettTALuc)1Dgs;   tetO;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Mating SystemHomozygote x Homozygote         (Female x Male)
Specieslaboratory mouse
Donating Investigator David Schatz,   Yale University School of Medicine
Generation[B6N3F3p]N2F7 (27-NOV-06)

Strain Description
Transgenic mice were produced by co-microinjection of modified tetracycline-controlled transactivator (tTA) and luciferase genes placed under the control of tetracycline-responsive promoter elements (TREs). Expression of tTA in transgenic mice is both inducible and autoregulatory; expression of the transgenes are suppressed in the presence of tetracycline and highly expressed in its absence. Induced transgene expression levels are higher than those achieved in strains utilizing a constitutive tTA system. Luciferase expression (in the absence of tetracycline) has been demonstrated in all organs examined - spleen, thymus, lung, liver, kidney, heart, cerebrum, cerebellum, lymph nodes, testes. High activity is found in thymus and lung; low expression is found in liver and kidney. Induction ranges from 2-fold in testes to 150-fold in thymus. The founder line of this strain is #17, as reported in the primary reference. Homozygous mice are viable and fertile.

Strain Development
(C57BL/6 x C3H) F1 hybrid fertilized embryos were co-microinjected with DNA constructs that direct the expression of the tetracycline-controlled transactivator (tTA) and luciferase genes under the control of tetracycline-responsive promoter elements (TREs). The strain corresponds to founder line #17 described in the primary reference.

Following the strains arrival at The Jackson Laboratory, it was backcrossed for three generations to C57BL/6 before being intercrossed to produce homozygotes. In 2004, transgenic mice recovered from cryopreservation were outcrossed for two generations to B6C3F1/J (Stock No. 100010) before being re-bred to homozygosity.

Gene & Allele Details

Allele Symbol Tg(TettTALuc)1Dgs
Allele Name transgene insertion 1, David Schatz
Mutation Made By David Schatz,   Yale University School of Medicine
Site of ExpressionInducible and autoregulatory in most tissues; in the absence of tetracycline, expression highest in thymus, lung.
Expressed Gene luc, luciferase, firefly
Expressed Gene tTA, tetracycline-controlled transactivator, E. coli
The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter.
Promoter tetO, tet operator,
General Note This line was generated from founder #17. Homozygous transgenic mice are viable and fertile. Expression of tTA in transgenic mice is both inducible and autoregulatory; expression of the transgenes are suppressed in the presence of tetracycline and highlyexpressed in its absence. Levels of induced transgene expression in these mice are higher than those achieved in mice carrying a transgene that utilizes a constitutive tTA system. Luciferase (in the absence of tetracycline) is expressed in spleen, thymus, lung, liver, kidney, heart, cerebrum, cerebellum, lymph nodes, and testes. High activity is found in thymus and lung; low expression is found in liver and kidney. Induction ranges from 2-fold in testes to 150-fold in thymus.
Molecular Note Transgenic mice were produced by co-microinjection of modified tetracycline-controlled transactivator (tTA) and luciferase genes placed under the control of tetracycline-responsive promoter elements (TREs). [MGI Ref ID J:95091]

Control Information

  Control
   001022 B6C3FeF1/J a/a (approximate)
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(TettTALuc)1Dgs QPCR

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Related Strains

View Strains carrying other alleles of luc     (10 strains)

View Strains carrying other alleles of tTA     (19 strains)

View Strains carrying other alleles of tetO     (27 strains)

Additional Web Information

Tet Expression Systems

Animal Health Reports

Room Number           AX12

Research Applications

This mouse can be used to support research in many areas including:

Research Tools
Reproductive Biology Research (Tetop Tet System)
Tet Expression Systems (tTA/rtTA Expressing Strains)
Tet Expression Systems (tTA/rtTA Responsive Strains)

References

Selected Reference(s)

Shockett P; Difilippantonio M; Hellman N; Schatz DG. 1995. A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. Proc Natl Acad Sci U S A 92(14):6522-6. [PubMed: 7604026]  [MGI Ref ID J:95091]

Additional References

Price and Supply Information

Strain Name: B6;C3-Tg(TettTALuc)1Dgs/J
Stock Number: 002709

Price Details

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Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes Usually shipped between four and eight weeks of age.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.
LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.

For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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