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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
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Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N1 (11-JUL-04) Donating Investigator David Schatz, Yale University School of Medicine, HHMI Description
Transgenic mice were produced by co-microinjection of modified tetracycline-controlled transactivator (tTA) and luciferase genes placed under the control of tetracycline-responsive promoter elements (TREs). Expression of tTA in transgenic mice is both inducible and autoregulatory; expression of the transgenes are suppressed in the presence of tetracycline and highly expressed in its absence. Induced transgene expression levels are higher than those achieved in strains utilizing a constitutive tTA system. Luciferase expression (in the absence of tetracycline) has been demonstrated in all organs examined - spleen, thymus, lung, liver, kidney, heart, cerebrum, cerebellum, lymph nodes, testes. High activity is found in thymus and lung; low expression is found in liver and kidney. Induction ranges from 2-fold in testes to 150-fold in thymus. The founder line of this strain is #17, as reported in the primary reference. Homozygous mice are viable and fertile.Development
(C57BL/6 x C3H) F1 hybrid fertilized embryos were co-microinjected with DNA constructs that direct the expression of the tetracycline-controlled transactivator (tTA) and luciferase genes under the control of tetracycline-responsive promoter elements (TREs). The strain corresponds to founder line #17 described in the primary reference.Following the strains arrival at The Jackson Laboratory, it was backcrossed for three generations to C57BL/6 before being intercrossed to produce homozygotes. In 2004, transgenic mice recovered from cryopreservation were outcrossed for two generations to B6C3F1/J (Stock No. 100010) before being re-bred to homozygosity.
Control Information
| Control | ||
|---|---|---|
| 001022 B6C3FeF1/J a/a | (approximate) | |
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of luc
006100 B10.Cg-H2k Tg(NFkB/Fos-luc)26Rinc/J 006102 B10.Cg-H2k Tg(Il2/NFAT-luc)83Rinc/J 005999 B6(SJL)-Tg(SBE/Tk-luc)7Twc/J 003479 B6.C3-Tg(Fos-luc)1Rnd/J 006098 B6.Cg-Tg(Il2/NFAT-luc)83Rinc/J 006851 B6.Cg-Tg(Per1-luc)025Jt/J 006101 B6.Cg-Tg(TRE/Prl-luc)31FlvRinc/J 008450 FVB-Tg(CAG-luc,-GFP)L2G85Chco/J 009638 FVB-Tg(GFAP-luc,GAPDH-rluc)172.9Mes/J 005125 FVB.129S6(B6)-Gt(ROSA)26Sortm1(Luc)Kael/J 002060 FVB/N-Tg(HIV-luc)326Morr/J 007800 FVB/N-Tg(Ins1-luc)VUPwrs/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J View Strains carrying other alleles of luc (14 strains)
007004 B6.Cg-Tg(Camk2a-tTA)1Mmay/DboJ 003767 B6.Cg-Tg(Eno2tTA)5021Nes/J 003763 B6.Cg-Tg(Eno2tTA)5030Nes/J 005964 B6.Cg-Tg(GFAP-tTA)110Pop/J 002618 B6.Cg-Tg(MMTVtTA)1Mam/J 008284 B6.Cg-Tg(Scg2-tTA)1Jt/J 006361 B6.Cg-Tg(Sp7-tTA,tetO-EGFP/cre)1Amc/J 003563 B6.Cg-Tg(tTALap)5Bjd/J 003010 B6;CBA-Tg(Camk2a-tTA)1Mmay/J 008344 B6;DBA-Tg(Fos-tTA,Fos-EGFP*)1Mmay Tg(tetO-lacZ,tTA*)1Mmay/J 010573 B6;SJL-Tg(Prl-tTA)6-5Jek/J 008082 B6;SJL-Tg(Tagln-tTA)1Mrab Tg(tetO-Mcpt1)1Mrab/J 005625 FVB-Tg(Pcp2-tTA)3Horr/J 003170 FVB.Cg-Tg(Myh6-tTA)6Smbf/J 006209 FVB.Cg-Tg(Tal1-tTA)19Dgt/J 005942 FVB/N-Tg(Pf4-tTA/VP16)42Kra/J 004937 NOD.Cg-Tg(Ins2-tTA)1Doi/DoiJ 006999 STOCK Dbttm1Geh Tg(tTALap)5Bjd Tg(tetO-DBT)A1Geh/J 009606 STOCK Tg(Six2-EGFP/cre)1Amc/J 003271 STOCK Tg(tTAhCMV)3Bjd/J 003275 STOCK Tg(tetL)1Bjd/J 003274 STOCK Tg(tetNZL)2Bjd/J
Additional Web Information
Tet Expression Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
Research Tools
Reproductive Biology Research
Tetop Tet System
Tet Expression Systems
tTA/rtTA Expressing Strains
tTA/rtTA Responsive Strains
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TettTALuc)1Dgs | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, David Schatz | ||
| Allele Type | Transgenic (Reporter) | ||
| Mutation Made By | David Schatz, Yale University School of Medicine, HHMI | ||
| Site of Expression | Inducible and autoregulatory in most tissues; in the absence of tetracycline, expression highest in thymus, lung. | ||
| Expressed Gene | tTA, tetracycline-controlled transactivator, E. coli | ||
| The tetracycline-resistance gene (TetR), arose from chemically mutated Escherichia coli genome which was screened for tetracycline dependence (Gossen and Bujard, 1992). TetR was fused at the C-terminus with the viral co-activator, virion protein 16 of the herpes simplex virus (VP-16). The tetracycline-inhibitable transcription factor is a component of a bigenic system that allows doxycycline (a tetracycline analog) regulatable expression of genes that are under the direction of the tetracycline responsive promoter (TetOp)promoter. | |||
| Expressed Gene | luc, luciferase, firefly | ||
| Promoter | tetO, tet operator, | ||
| Gene Symbol and Name | Tg(TettTALuc)1Dgs, transgene insertion 1, David Schatz | ||
| Chromosome | UN | ||
| General Note | This line was generated from founder #17. Homozygous transgenic mice are viable and fertile. Expression of tTA in transgenic mice is both inducible and autoregulatory; expression of the transgenes are suppressed in the presence of tetracycline and highlyexpressed in its absence. Levels of induced transgene expression in these mice are higher than those achieved in mice carrying a transgene that utilizes a constitutive tTA system. Luciferase (in the absence of tetracycline) is expressed in spleen, thymus, lung, liver, kidney, heart, cerebrum, cerebellum, lymph nodes, and testes. High activity is found in thymus and lung; low expression is found in liver and kidney. Induction ranges from 2-fold in testes to 150-fold in thymus. | ||
| Molecular Note | Transgenic mice were produced by co-microinjection of modified tetracycline-controlled transactivator (tTA) and luciferase genes placed under the control of tetracycline-responsive promoter elements (TREs). [MGI Ref ID J:95091] | ||
Genotyping
Genotyping Information
Genotyping Protocols
Tg(TettTALuc)1Dgs, QPCR
Tg(luc), Standard PCR
Tg(tTA), Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Shockett P; Difilippantonio M; Hellman N; Schatz DG. 1995. A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. Proc Natl Acad Sci U S A 92(14):6522-6. [PubMed: 7604026] [MGI Ref ID J:95091]
Additional References
Tg(TettTALuc)1Dgs relatedShockett PE; Zhou S; Hong X; Schatz DG. 2004. Partial reconstitution of V(D)J rearrangement and lymphocyte development in RAG-deficient mice expressing inducible, tetracycline-regulated RAG transgenes. Mol Immunol 40(11):813-29. [PubMed: 14687938] [MGI Ref ID J:87913]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001022 B6C3FeF1/J a/a | (approximate) | |
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
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Terms of Use
Terms of Use
General Terms and Conditions
Use of the Tet-System may require a license, see Licenses for Strains Using TET-System Technology.
For additional Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
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