Strain Name:

B6;129-Gabrb3tm1Geh/J

Stock Number:

002711

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Availability:

Cryopreserved - Ready for recovery

These beta3-mutant mice lack the beta3 coding region of the Gabrb3 (gamma-aminobutyric acid (GABA-A) receptor, subunit beta 3) locus and may be useful in studying neurodevelopmental disorders such as cleft palate, seizures, epilepsy, and sensitivity to anesthetics and ethanol. In addition, the observed behavioral deficits (especially regarding social behaviors) indicate that mutant mice may be a useful model of autism spectrum disorders.

Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating InvestigatorDr. Gregg Homanics,   University of Pittsburgh

Description
The gamma-Aminobutyric acid type A receptors mediate the majority of rapid inhibitory synaptic transmission in the CNS. The beta3 subunit is an essential component of these receptors in many brain regions, especially during development, and is implicated in several pathophysiologic processes. The majority of mice homozygous for the Gabrb3tm1Geh mutation (or beta3-/-) die at birth with ~60% displaying cleft palate and the remaining ~35% die for unidentified reasons. Homozygous females that survive are fertile but do not care for their pups. Survivors have frequent myoclonus and occasional epileptic seizures, are hypersensitive to external stimuli and handling, have a lack of coordination and display altered responses to certain anesthesias. In addition, the observed behavioral deficits (especially regarding social behaviors) indicate that mutant mice may be a useful model of autism spectrum disorders.

Of note, several strains bearing gamma-aminobutyric acid (GABA-A) receptor mutations are available from this donating investigator (Dr. Gregg Homanics, University of Pittsburgh), including Gabra1 (Stock No. 004318), Gabra4 (Stock No. 006874), Gabra6 (Stock No. 002710), Gabrb3 (Stock No. 002711), Gabrd (Stock No. 003725), and Gabrg2 (Stock No. 003137).

Development
This strain was developed in the laboratory of Dr. Gregg Homanics at the University of Pittsburgh. The vector containing Pgk-neo was inserted by homologous recombination and resulted in deletion of the promoter and exons 1 and 2 of the Gabrb3 gene. The 129X1/SvJ x 129S1/Sv-derived R1 ES cell line was used.

Control Information

  Control
   Wild-type from the colony
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Related Strains

Facebase: models
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002619   B6.129-Tgfb3tm1Doe/J
007453   B6.129P2(Cg)-Dhcr7tm1Gst/J
010525   B6.129S-Notch2tm3Grid/J
010616   B6.129S1-Jag1tm1Grid/J
010546   B6.129S1-Jag2tm1Grid/J
010620   B6.129S1-Notch2tm1Grid/J
009387   B6.129S1-Osr1tm1Jian/J
009386   B6.129S1-Osr2tm1Jian/J
010621   B6.129S1-Snai1tm2.1Grid/J
010617   B6.129S1-Snai2tm1Grid/J
003865   B6.129S2-Itgavtm1Hyn/J
003755   B6.129S4-Meox2tm1(cre)Sor/J
016902   B6.129S5-Irf6Gt(OST398253)Lex/J
003336   B6.129S7-Cdkn1ctm1Sje/J
012843   B6.129X1(Cg)-Slc32a1tm1.1Bgc/J
000026   B6.C3-Gli3Xt-J/J
004275   B6.Cg-Fignfi/Frk
012844   B6.Cg-Gad1tm1.1Bgc/J
006382   B6;129-Casktm1Sud/J
004293   B6;129-Shhtm2Amc/J
012603   B6;129-Tgfbr2tm1Karl/J
010618   B6;129S-Jag1tm2Grid/J
010686   B6;129S-Snai1tm2Grid/J
009389   B6;129S1-Bambitm1Jian/J
010619   B6;129S1-Lfngtm1Grid/J
010547   B6;129S1-Notch3tm1Grid/J
010544   B6;129S1-Notch4tm1Grid/J
010722   B6;129S1-Snai2tm2Grid/J
012463   B6;129S4-Foxd1tm1(GFP/cre)Amc/J
022358   B6;129S6-Rr23tm1Axvi/Mmjax
022359   B6;129S6-Rr24tm1Axvi/Mmjax
022360   B6;129S6-Rr25tm1Axvi/Mmjax
003277   B6;129S7-Acvr2atm1Zuk/J
002788   B6;129S7-Fsttm1Zuk/J
002990   B6;129S7-Inhbatm1Zuk/J
000523   B6By.Cg-Eh/J
000278   B6C3Fe a/a-Papss2bm Hps1ep Hps6ru/J
000515   B6CBACa Aw-J/A-SfnEr/J
001434   C3HeB/FeJ x STX/Le-Mc1rE-so Gli3Xt-J Zeb1Tw/J
000252   DC/LeJ
005057   FVB.129-Kcnj2tm1Swz/J
012655   FVB.A-Irf6clft1/BeiJ
013100   FVB.C-Prdm16csp1/J
017437   FVB/N-Ckap5TgTn(sb-cHS4,Tyr)2320F-1Ove/J
017438   FVB/N-MidnTg(Tyr)2261EOve/J
017609   FVB/N-Rr16Tn(sb-Tyr)1HCebOve/J
017598   FVB/N-Sdccag8Tn(sb-Tyr)2161B.CA1C2Ove/J
017608   FVB/N-Skor2Tn(sb-Tyr)1799B.CA7BOve/J
017436   FVB/N-Tapt1TgTn(sb-cHS4,Tyr)2508GOve/J
016870   FVB/NJ-Ap2b1Tg(Tyr)427Ove/EtevJ
017434   FVB;B6-Cramp1lTgTn(sb-rtTA,Tyr)2447AOve/J
017594   FVB;B6-Eya4TgTn(Prm1-sb10,sb-Tyr)1739AOve/J
017435   FVB;B6-SlmapTn(sb-rtTA)2426B.SB4Ove/J
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003102   STOCK Tgfb2tm1Doe/J
018624   STOCK Tgfb3tm2(Tgfb1)Vk/J
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View Facebase: models     (61 strains)

Strains carrying other alleles of Gabrb3
008310   B6;129-Gabrb3tm2.1Geh/J
View Strains carrying other alleles of Gabrb3     (1 strain)

Additional Web Information

JAX® NOTES, Winter 2008; 512. JAX® Mice strain sheds new light on autism.

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Autism
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Epilepsy, Childhood Absence, Susceptibility to, 5; ECA5   (GABRB3)
Gamma-Aminobutyric Acid Receptor, Beta-3; GABRB3   (GABRB3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Gabrb3tm1Geh/Gabrb3tm1Geh

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6J
  • behavior/neurological phenotype
  • abnormal behavior
    • frequently seen sleeping away from nesting material   (MGI Ref ID J:130259)
    • abnormal response to novel object
      • increased time until first contact with a novel object compared to wild-type   (MGI Ref ID J:130259)
      • decreased amount of time spent investigating a novel object compared to wild-type   (MGI Ref ID J:130259)
    • abnormal social/conspecific interaction
      • fail to show a significant preference for spending time in the interaction chamber adjacent to the stimulus cage containing an unfamiliar mouse as opposed to spending time in the interaction chamber adjacent to an empty stimulus cage   (MGI Ref ID J:130259)
      • mice fail to exhibit a preference for interacting with a new mouse over a familiar mouse   (MGI Ref ID J:130259)
      • abnormal nest building behavior
        • at 1, 24 and 48 hours after introduction of new nestlet material, mice utilize significantly less material than wild-type controls   (MGI Ref ID J:130259)
        • significant impairment in their ability to achieve the nest complexity exhibited by the controls   (MGI Ref ID J:130259)
        • location of nest construction is different   (MGI Ref ID J:130259)
    • circling   (MGI Ref ID J:130259)
    • decreased vertical activity
      • total number of rearings differs from wild-type littermates and C57BL/6J mice but not from 129X1/SvJ mice   (MGI Ref ID J:130259)
      • the mean duration of each rearing event is decreased compared to controls   (MGI Ref ID J:130259)
    • hyperactivity
      • a higher degree of ambulatory activity than control mice   (MGI Ref ID J:130259)
      • a higher number of entries, in regards to the total transitions between all chambers, of a social interaction apparatus   (MGI Ref ID J:130259)
  • nervous system phenotype
  • abnormal cerebellum vermis lobule morphology   (MGI Ref ID J:130259)
    • abnormal cerebellum vermis lobule II morphology
      • significant decrease in the surface area   (MGI Ref ID J:130259)
    • abnormal cerebellum vermis lobule III morphology
      • significant decrease in the surface area   (MGI Ref ID J:130259)
    • abnormal cerebellum vermis lobule IV morphology
      • significant decrease in the surface area   (MGI Ref ID J:130259)
    • abnormal cerebellum vermis lobule V morphology
      • significant decrease in the surface area   (MGI Ref ID J:130259)
    • abnormal cerebellum vermis lobule VI morphology
      • significant decrease in the surface area   (MGI Ref ID J:130259)
    • abnormal cerebellum vermis lobule VII morphology
      • significant decrease in the surface area   (MGI Ref ID J:130259)

Gabrb3tm1Geh/Gabrb3tm1Geh

        involves: 129S1/Sv * 129X1/SvJ * C57BL/6
  • mortality/aging
  • partial perinatal lethality
    • 30% with normal palates die neonatally   (MGI Ref ID J:39801)
    • 90% die within 1 day of birth   (MGI Ref ID J:39801)
  • premature death
    • animals surviving to birth live an average of 18 weeks   (MGI Ref ID J:39801)
  • growth/size/body phenotype
  • decreased body size
    • 5-10% that survive to adulthood are runted until weaning but achieve normal body weight by adulthood   (MGI Ref ID J:39801)
  • behavior/neurological phenotype
  • abnormal motor capabilities/coordination/movement   (MGI Ref ID J:39801)
    • circling   (MGI Ref ID J:108890)
      • many have been observed to run continuously in tight circles for extended periods of time   (MGI Ref ID J:39801)
    • hyperactivity   (MGI Ref ID J:39801)
    • impaired coordination
      • have difficulty walking on grids and repeatedly fall off platforms and rotarods   (MGI Ref ID J:39801)
    • impaired swimming
      • have difficulty swimming   (MGI Ref ID J:39801)
    • limb grasping   (MGI Ref ID J:39801)
  • abnormal parental behavior
    • failure to nuture offspring   (MGI Ref ID J:39801)
  • hyperresponsive
    • hyperresponsive to human contact and other sensory stimuli   (MGI Ref ID J:39801)
  • seizures
    • variable severity of epileptic seizures in mice over 10 weeks of age   (MGI Ref ID J:39801)
  • hearing/vestibular/ear phenotype
  • abnormal auditory brainstem response waveform shape
    • at 6 weeks, suprathreshold ABR amplitudes in homozygotes are significantly reduced in amplitude, by at least 50% at all test frequencies and sound levels   (MGI Ref ID J:112951)
  • abnormal cochlea morphology
    • at >24 weeks, homozygotes exhibit basal-turn histopathology   (MGI Ref ID J:112951)
    • rarity and fragility of homozygotes prevented evaluation of cochlear histopathology at 6 weeks, as none were killed at this age   (MGI Ref ID J:112951)
    • abnormal cochlear IHC afferent innervation pattern
      • at >24 weeks, homozygotes display a significant reduction in the density of IHC afferent innervation relative to age-matched wild-type mice, indicating neuropathy   (MGI Ref ID J:112951)
    • abnormal cochlear OHC efferent innervation pattern
      • at >24 weeks, homozygotes display a reduction in OHC efferent innervation, with fewer efferent axons crossing to OHCs at the level of the tunnel of Corti relative to age-matched wild-type mice   (MGI Ref ID J:112951)
    • cochlear inner hair cell degeneration
      • at ~24 weeks, homozygotes display a nearly complete loss of IHCs throughout the basal 15% of the cochlear spiral, i.e., from 40 to 90 kHz according to the mouse cochlear frequency map   (MGI Ref ID J:112951)
    • cochlear outer hair cell degeneration
      • at ~24 weeks, homozygotes display a nearly complete loss of OHCs throughout the basal 15% of the cochlear spiral, i.e., from 40 to 90 kHz according to the mouse cochlear frequency map   (MGI Ref ID J:112951)
    • type IV spiral ligament fibrocyte degeneration
      • at >24 weeks, homozygotes display widespread loss of type IV fibrocytes, spreading farther apically   (MGI Ref ID J:112951)
  • abnormal distortion product otoacoustic emission
    • at 6 weeks, homozygotes display significantly elevated DPOAE thresholds across all test frequencies relative to wild-type mice, with DPOAE thresholds of >60 dB at high frequencies   (MGI Ref ID J:112951)
    • at 16 kHz (middle cochlear region), ABR shifts are similar in magnitude to the observed DPOAE shifts, suggesting that the threshold shift arises at, or before, the stage of OHC transduction and amplification   (MGI Ref ID J:112951)
  • increased or absent threshold for auditory brainstem response
    • at 6 weeks, homozygotes display severe cochlear dysfunction, as shown by highly significant ABR threshold elevations of 20-45 dB across a range of test frequencies, with peak loss occurring at 22.6 kHz   (MGI Ref ID J:112951)
    • ABR threshold elevation corresponds to >2 orders of magnitude of stimulus amplitude   (MGI Ref ID J:112951)
  • sensorineural hearing loss   (MGI Ref ID J:112951)
  • nervous system phenotype
  • abnormal CNS synaptic transmission
    • 80% decrease in the maximal amplitude of GABA-activated chloride currents in dorsal root ganglion   (MGI Ref ID J:39801)
  • abnormal cochlear IHC afferent innervation pattern
    • at >24 weeks, homozygotes display a significant reduction in the density of IHC afferent innervation relative to age-matched wild-type mice, indicating neuropathy   (MGI Ref ID J:112951)
  • abnormal cochlear OHC efferent innervation pattern
    • at >24 weeks, homozygotes display a reduction in OHC efferent innervation, with fewer efferent axons crossing to OHCs at the level of the tunnel of Corti relative to age-matched wild-type mice   (MGI Ref ID J:112951)
  • abnormal cochlear nerve morphology
    • at >24 weeks, homozygotes display clear signs of cochlear nerve abnormalities (progressive loss of afferent terminals)   (MGI Ref ID J:112951)
  • cochlear ganglion degeneration
    • at >24 weeks, homozygotes show partial loss of spiral ganglion cells but only in cochlear regions in which IHCs are degenerated   (MGI Ref ID J:112951)
  • cochlear inner hair cell degeneration
    • at ~24 weeks, homozygotes display a nearly complete loss of IHCs throughout the basal 15% of the cochlear spiral, i.e., from 40 to 90 kHz according to the mouse cochlear frequency map   (MGI Ref ID J:112951)
  • cochlear outer hair cell degeneration
    • at ~24 weeks, homozygotes display a nearly complete loss of OHCs throughout the basal 15% of the cochlear spiral, i.e., from 40 to 90 kHz according to the mouse cochlear frequency map   (MGI Ref ID J:112951)
  • seizures
    • variable severity of epileptic seizures in mice over 10 weeks of age   (MGI Ref ID J:39801)
  • small cochlear ganglion
    • mutant calretinin-negative cells are smaller at each sampled location in the spiral ganglion relative to wild-type cells   (MGI Ref ID J:108890)
    • the reduction in mean somatic area of mutant calretinin-negative cells is 5.2% in the basal turn, 8.7% in the middle turn, and 12.2% in the apical turn of the spiral ganglion relative to wild-type cells   (MGI Ref ID J:108890)
    • adult homozygotes display significantly smaller cochlear ganglion cell areas at every sampled region in the spiral ganglion, except the apical cochlear turn   (MGI Ref ID J:108890)
    • in contrast, mutant calretinin-positive ganglion cells are of normal size and proportion relative to wild-type cells   (MGI Ref ID J:108890)
    • cochlear ganglion hypoplasia
      • adult homozygotes show hypoplasia of spiral ganglion cells in the middle and basal turns of the cochlea, while the apical turn remains unaffected   (MGI Ref ID J:108890)
      • spiral ganglion cell hypoplasia is strictly due to a size reduction of calretinin-negative ganglion cells   (MGI Ref ID J:108890)
  • small vestibular ganglion
    • adult homozygotes display significantly smaller ganglion cell areas in both divisions of Scarpa's ganglion (superior and inferior) relative to wild-type mice   (MGI Ref ID J:108890)
    • mutant calretinin-negative cells are smaller in both divisions of Scarpa's ganglion relative to wild-type cells   (MGI Ref ID J:108890)
    • the reduction in mean somatic area of mutant calretinin-negative cells is 9.5% in the inferior division and 21.5% in the superior division of Scarpa's ganglion   (MGI Ref ID J:108890)
    • in contrast, mutant calretinin-positive ganglion cells are of normal size and proportion relative to wild-type cells   (MGI Ref ID J:108890)
    • vestibular ganglion hypoplasia
      • adult homozygotes show ganglion cell hypoplasia in both divisions of Scarpa's ganglion   (MGI Ref ID J:108890)
      • Scarpa ganglion cell hypoplasia is strictly due to a size reduction of calretinin-negative ganglion cells   (MGI Ref ID J:108890)
  • craniofacial phenotype
  • cleft palate
    • about 57% exhibit cleft palate   (MGI Ref ID J:39801)
  • digestive/alimentary phenotype
  • cleft palate
    • about 57% exhibit cleft palate   (MGI Ref ID J:39801)
  • skeleton phenotype
  • type IV spiral ligament fibrocyte degeneration
    • at >24 weeks, homozygotes display widespread loss of type IV fibrocytes, spreading farther apically   (MGI Ref ID J:112951)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Neurobiology Research
Epilepsy
Neurodevelopmental Defects
      Autism
Receptor Defects
      GABA receptor

Gabrb3tm1Geh related

Cell Biology Research
Channel and Transporter Defects
      chloride
      chloride: GABAA receptor

Developmental Biology Research
Neurodevelopmental Defects

Neurobiology Research
Behavioral and Learning Defects
Channel and Transporter Defects
      chloride
      chloride: GABAA receptor
Epilepsy
Neurodevelopmental Defects
Neurotransmitter Receptor and Synaptic Vesicle Defects
Receptor Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gabrb3tm1Geh
Allele Name targeted mutation 1, Gregg E Homanics
Allele Type Targeted (knock-out)
Common Name(s) beta3-;
Mutation Made ByDr. Gregg Homanics,   University of Pittsburgh
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Gabrb3, gamma-aminobutyric acid (GABA) A receptor, subunit beta 3
Chromosome 7
Gene Common Name(s) A230092K12Rik; AW049585; Cp1; ECA5; Gabrb-3; RIKEN cDNA A230092K12 gene; beta3; cleft palate 1; expressed sequence AW049585;
Molecular Note A neomycin resistance cassette replaced 2.8 kb of sequence, including exons 1-3. [MGI Ref ID J:39801]

Genotyping

Genotyping Information

Genotyping Protocols

Gabrb3tm1Geh, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Delorey TM; Sahbaie P; Hashemi E; Homanics GE; Clark JD. 2008. Gabrb3 gene deficient mice exhibit impaired social and exploratory behaviors, deficits in non-selective attention and hypoplasia of cerebellar vermal lobules: A potential model of autism spectrum disorder. Behav Brain Res 187(2):207-20. [PubMed: 17983671]  [MGI Ref ID J:130259]

Homanics GE; DeLorey TM; Firestone LL; Quinlan JJ; Handforth A ; Harrison NL ; Krasowski MD ; Rick CE ; Korpi ER ; Makela R ; Brilliant MH ; Hagiwara N ; Ferguson C ; Snyder K ; Olsen RW. 1997. Mice devoid of gamma-aminobutyrate type A receptor beta3 subunit have epilepsy, cleft palate, and hypersensitive behavior. Proc Natl Acad Sci U S A 94(8):4143-8. [PubMed: 9108119]  [MGI Ref ID J:39801]

Additional References

Dellovade TL; Davis AM; Ferguson C; Sieghart W; Homanics GE; Tobet SA. 2001. GABA influences the development of the ventromedial nucleus of the hypothalamus. J Neurobiol 49(4):264-76. [PubMed: 11745664]  [MGI Ref ID J:79217]

Gabrb3tm1Geh related

DeLorey TM; Handforth A; Anagnostaras SG; Homanics GE; Minassian BA ; Asatourian A ; Fanselow MS ; Delgado-Escueta A ; Ellison GD ; Olsen RW. 1998. Mice lacking the beta3 subunit of the GABAA receptor have the epilepsy phenotype and many of the behavioral characteristics of Angelman syndrome. J Neurosci 18(20):8505-14. [PubMed: 9763493]  [MGI Ref ID J:50564]

DeLorey TM; Sahbaie P; Hashemi E; Li WW; Salehi A; Clark DJ. 2011. Somatosensory and sensorimotor consequences associated with the heterozygous disruption of the autism candidate gene, Gabrb3. Behav Brain Res 216(1):36-45. [PubMed: 20699105]  [MGI Ref ID J:174161]

Hagiwara N; Katarova Z; Siracusa LD; Brilliant MH. 2003. Nonneuronal expression of the GABA(A) beta3 subunit gene is required for normal palate development in mice. Dev Biol 254(1):93-101. [PubMed: 12606284]  [MGI Ref ID J:83131]

Hashemi E; Sahbaie P; Davies MF; Clark JD; DeLorey TM. 2007. Gabrb3 gene deficient mice exhibit increased risk assessment behavior, hypotonia and expansion of the plexus of locus coeruleus dendrites. Brain Res 1129(1):191-9. [PubMed: 17156762]  [MGI Ref ID J:117697]

Huntsman MM; Porcello DM; Homanics GE; DeLorey TM; Huguenard JR. 1999. Reciprocal inhibitory connections and network synchrony in the mammalian thalamus. Science 283(5401):541-3. [PubMed: 9915702]  [MGI Ref ID J:124675]

Koo JW; Homanics GE; Balaban CD. 2002. Hypoplasia of spiral and Scarpa's ganglion cells in GABA(A) receptor beta(3) subunit knockout mice. Hear Res 167(1-2):71-80. [PubMed: 12117532]  [MGI Ref ID J:108890]

Krasowski MD; Rick CE; Harrison NL; Firestone LL; Homanics GE. 1998. A deficit of functional GABA(A) receptors in neurons of beta 3 subunit knockout mice. Neurosci Lett 240(2):81-4. [PubMed: 9486477]  [MGI Ref ID J:107951]

Liljelund P; Handforth A; Homanics GE; Olsen RW. 2005. GABA(A) receptor beta3 subunit gene-deficient heterozygous mice show parent-of-origin and gender-related differences in beta3 subunit levels, EEG, and behavior. Brain Res Dev Brain Res 157(2):150-61. [PubMed: 15878204]  [MGI Ref ID J:99362]

Maison SF; Rosahl TW; Homanics GE; Liberman MC. 2006. Functional role of GABAergic innervation of the cochlea: phenotypic analysis of mice lacking GABA(A) receptor subunits alpha1, alpha2, alpha5, alpha6, beta2, beta3, or delta. J Neurosci 26(40):10315-26. [PubMed: 17021187]  [MGI Ref ID J:112951]

Nusser Z; Kay LM; Laurent G; Homanics GE; Mody I. 2001. Disruption of GABA(A) receptors on GABAergic interneurons leads to increased oscillatory power in the olfactory bulb network. J Neurophysiol 86(6):2823-33. [PubMed: 11731539]  [MGI Ref ID J:104000]

Olexova L; Talarovicova A; Lewis-Evans B; Borbelyova V; Krskova L. 2012. Animal models of autism with a particular focus on the neural basis of changes in social behaviour: an update article. Neurosci Res 74(3-4):184-94. [PubMed: 23142422]  [MGI Ref ID J:192363]

Quinlan JJ; Homanics GE; Firestone LL. 1998. Anesthesia sensitivity in mice that lack the beta3 subunit of the gamma-aminobutyric acid type A receptor. Anesthesiology 88(3):775-80. [PubMed: 9523823]  [MGI Ref ID J:46728]

Ramadan E; Fu Z; Losi G; Homanics GE; Neale JH; Vicini S. 2003. GABA(A) receptor beta3 subunit deletion decreases alpha2/3 subunits and IPSC duration. J Neurophysiol 89(1):128-34. [PubMed: 12522165]  [MGI Ref ID J:102972]

Resnick A; Homanics GE; Jung BJ; Peris J. 1999. Increased acute cocaine sensitivity and decreased cocaine sensitization in GABA(A) receptor beta3 subunit knockout mice. J Neurochem 73(4):1539-48. [PubMed: 10501199]  [MGI Ref ID J:57686]

Sinkkonen ST; Homanics GE; Korpi ER. 2003. Mouse models of Angelman syndrome, a neurodevelopmental disorder, display different brain regional GABA(A) receptor alterations. Neurosci Lett 340(3):205-8. [PubMed: 12672542]  [MGI Ref ID J:108025]

Ugarte SD; Homanics GE; Firestone LL; Hammond DL. 2000. Sensory thresholds and the antinociceptive effects of GABA receptor agonists in mice lacking the beta3 subunit of the GABA(A) receptor Neuroscience 95(3):795-806. [PubMed: 10670447]  [MGI Ref ID J:60491]

Ugarte SD; Homanics GE; Hammond DL. 2001. Effect of embryonic knock-down of GABA(A) receptors on the levels of monoamines and their metabolites in the CNS of the mouse. Brain Res 904(2):290-7. [PubMed: 11406127]  [MGI Ref ID J:70367]

Wisor JP; DeLorey TM; Homanics GE; Edgar DM. 2002. Sleep states and sleep electroencephalographic spectral power in mice lacking the beta(3) subunit of the GABA(A) receptor. Brain Res 955(1-2):221-8. [PubMed: 12419540]  [MGI Ref ID J:80484]

Wong SM; Cheng G; Homanics GE; Kendig JJ. 2001. Enflurane actions on spinal cords from mice that lack the beta3 subunit of the GABA(A) receptor. Anesthesiology 95(1):154-64. [PubMed: 11465553]  [MGI Ref ID J:106235]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryThis strain is currently maintained on a mixed C57BL/6J x 129 genetic background. It is maintained by mating heterozygous mice to normal wildtype siblings.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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