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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F5+p Donating Investigator Katya Ravid, Boston University School of Medicine Description
Mice homozygous for this transgene are viable and fertile. Expression of the transgene is exclusively in platelets and megakaryocytes in female carrier mice or in male carrier mice given injections of estrogen. Megakaryopoiesis is increased and they exhibit essential thrombocythemia. This strains serves as a model for a myeloproliferative disorder resembling essential thrombocythemia. Also known as PF4MER.
Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of ESR1
View Strains carrying other alleles of ESR1 (10 strains)
Phenotype
Phenotype Information
assigned by genotype
Tg(PF4MER)6Kra/?
FVB/N-Tg(PF4MER)6Kra
- immune system phenotype
- enlarged spleen (MGI Ref ID J:35507)
- spleens of pregnant transgenic mice are on average 24% bigger than in pregnant non-transgenic mice
- spleens of non-pregnant female mice are slightly larger than in controls
- hematopoietic system phenotype
- abnormal megakaryocyte morphology (MGI Ref ID J:35507)
- megakaryocytes from female mice are smaller
- megakaryocytes of the same ploidy state (16N) are about 10% smaller in female transgenic mice compared to female wild-type mice
- abnormal megakaryocyte differentiation (MGI Ref ID J:35507)
- megakaryocytes from bone marrow have higher rates of apoptosis in female transgenic mice compared to non-transgenic mice
- female transgenic mice have a significant decrease in the fraction of high ploidy (>32N) megakarocytes and an increased frequency of low ploidy megakarocytes (<8N)
- these differences in ploidy number are greater in pregnant transgenic mice when compared to pregnant non-transgenic mice
- increased megakaryocyte cell number (MGI Ref ID J:35507)
- an increased number of megakaryocytes are found in the spleen and bone marrow of female mice
- the number of megakaryocytes found in a female sternum bone marrow compartment is 65 compared to 30-35 in transgenic male mice or non-transgenic female mice
- pregnant female mice have an average of 76 megakarocytes per bone marrow compartment compared to 43 for pregnant non-transgenic females
- male mice treated with estrogen for two weeks increase the mean number of megakaryocytes to 61 per bone marrow compartment compared to 39 in estrogen-treated male wild-type mice
- enlarged spleen (MGI Ref ID J:35507)
- spleens of pregnant transgenic mice are on average 24% bigger than in pregnant non-transgenic mice
- spleens of non-pregnant female mice are slightly larger than in controls
This mouse can be used to support research in many areas including:
Cancer Research
Oncogenes
Hematological Research
Platelet Defects (thrombocytopenia)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(PF4MER)6Kra | ||
|---|---|---|---|
| Allele Name | transgene insertion 6, Katya Ravid | ||
| Allele Type | Transgenic (random, expressed) | ||
| Common Name(s) | PF4MERGH; | ||
| Mutation Made By | Katya Ravid, Boston University School of Medicine | ||
| Strain of Origin | FVB | ||
| Expressed Gene | MYC, v-myc myelocytomatosis viral oncogene homolog (avian), human | ||
| Expressed Gene | ESR1, estrogen receptor 1, human | ||
| Promoter | PF4, platelet factor 4, human | ||
| General Note | Mice homozygous for this transgene on an FVB/N background are viable and fertile. Expression of the transgene is exclusively in platelets and megakaryocytes in female carrier mice or in male carrier mice given injections of estrogen. Megakaryopoiesis is increased and they exhibit essential thrombocythemia. This strain may serve as a model for a myeloproliferative disorder resembling essential thrombocythemia. | ||
| Molecular Note | The transgene is a 3.9-kb fragment which contains the rat platelet factor 4 (Pf4) promoter followed by exons 2 and 3 of human myc fused to the human estrogen receptor (MER), followed by a 442-base pair region of the HGH poly(A) tail starting from the stop codon of the HGH gene. Two copies of the transgene integrated in this line. Expression of the transgene was detected in bone marrow megakaryocytes. [MGI Ref ID J:35507] | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Thompson A; Zhang Y; Kamen D; Jackson CW; Cardiff RD; Ravid K. 1996. Deregulated expression of c-myc in megakaryocytes of transgenic mice increases megakaryopoiesis and decreases polyploidization. J Biol Chem 271(38):22976-82. [PubMed: 8798484] [MGI Ref ID J:35507]
Health & husbandry
Health & Colony Maintenance Information
Currently there no information available for this strain. This may be due to the supply level of this strain.
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
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Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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