Description

Strain Information

Former Names STOCK hop/J    (Changed: 15-DEC-04 ) Type Congenic; Mutant Stock; Spontaneous Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain BALB/cByJ Donor Strain undefined mixed background Generation N15 (07-DEC-07)

Appearance
albino, ataxic
Related Genotype: Tyrp1^b/Tyrp1^b Tyr^c/Tyr^c hop/hop

albino, unaffected
Related Genotype: Tyrp1^b/Tyrp1^b Tyr^c/Tyr^c +/? or Tyrp1^b/Tyrp1^b Tyr^c/Tyr^c hop/+

Description
Mice homozygous for the hop-sterile spontaneous mutation (hop) are viable, females are fertile but males are sterile. Homozygous mutant mice walk with a characteristic hopping gait using the hindlegs simultaneously. There is preaxial polydactyly of both fore- and hindfeet. Defects in spermatogenesis result in abnormal sperm tail development. Tailed sperm are completely absent from the lumen of the testicular tubules. The second meiotic division is frequently abnormal or incomplete, often leaving four centrioles per cell. These centrioles usually fail to form flagella, and tail development is arrested.

Development
The hop-sterile (hop) mutation arose spontaneously in an unirradiated stock at the MRC Radiobiology Unit at Harwell in 1967. In 1996 this strain was imported to The Jackson Laboratory from Dr. Mary Ann Handel. At that time this mutation was not on a defined genetic background and the heterozygous offspring from the first backcross of this mixed background to BALB/c were received. These were intercrossed and the mutation has been maintained via backcross-intercross to BALB/cByJ ever since. In 1998 the strain reached N3, before the end of 1999 it reached N5, and by 2003 it had been backcrossed 11 times to BALB/cByJ.

Control Information

	Control
	+/? untyped from the colony
	Heterozygote from the colony
Considerations for Choosing Controls

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

hop/hop

        Background Not Specified

• behavior/neurological phenotype
• abnormal gait (MGI Ref ID J:5206)
  • homozygotes display a hopping gait in which the hind legs are move simultaneously
• abnormal posture (MGI Ref ID J:5206)
  • unlike in wild-type mice the legs in homozygous mice are not widely spread
• impaired swimming (MGI Ref ID J:5206)
  • the hindquaters are twisted and sinuous tail motions are absent when homozygotes swim
• endocrine/exocrine gland phenotype
• small testis (MGI Ref ID J:5206)
  • adult testes are smaller in mutants compared to wild-type littermates
• reproductive system phenotype
• abnormal spermiogenesis (MGI Ref ID J:5206)
  • cytokinesis is frequently interrupted at an early stage resulting in binucleated cells
  • spermatids with abnormal heads and tails are seen in homozygotes
  • microtubules of the manchette overproliferate and impinge on nuclear integrity in homozygotes
• azoospermia (MGI Ref ID J:5206)
  • no tailed sperm are found in the seminiferous tubules
  • a few elongated spermatids with abnormal flagella but no mature sperm are found in the seminiferous tubules of homozygotes
• male infertility (MGI Ref ID J:5206)
  • males are completely sterile
  • males are completely sterile
• small testis (MGI Ref ID J:5206)
  • adult testes are smaller in mutants compared to wild-type littermates
• limbs/digits/tail phenotype
• polydactyly (MGI Ref ID J:5206)
  • preaxial polydactyly of both hind- and forefeet

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

hop related

Developmental Biology Research
Skeletal Defects (polydactyly)

Neurobiology Research
Ataxia (Movement) Defects

Reproductive Biology Research
Developmental Defects Affecting Gonads (males only)
Fertility Defects (males only)

Genes & Alleles

Gene & Allele Information

Allele Symbol		hop
Allele Name		hop sterile
Allele Type		Spontaneous
Gene Symbol and Name		hop, hop-sterile
Chromosome		6
Gene Common Name(s)		hpy; hydrocephalic-polydactyl;
Molecular Note		The spontaneous mutation hop-sterile arose in an unirradiated stock at the MRC Radiobiology Unit at Harwell in 1967.

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Johnson DR; Hunt DM. 1971. Hop-sterile, a mutant gene affecting sperm tail development in the mouse. J Embryol Exp Morphol 25(2):223-36. [PubMed: 5088020]  [MGI Ref ID J:5206]

Additional References

Schumacher JM; Artzt K; Braun RE. 1998. Spermatid perinuclear ribonucleic acid-binding protein binds microtubules in vitro and associates with abnormal manchettes in vivo in mice. Biol Reprod 59(1):69-76. [PubMed: 9674995]  [MGI Ref ID J:89468]

hop related

Schumacher JM; Artzt K; Braun RE. 1998. Spermatid perinuclear ribonucleic acid-binding protein binds microtubules in vitro and associates with abnormal manchettes in vivo in mice. Biol Reprod 59(1):69-76. [PubMed: 9674995]  [MGI Ref ID J:89468]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           A2A

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Mouse Mutant Resource collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	+/? untyped from the colony
	Heterozygote from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.