Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation N?+6   Donating Investigator IMR Colony,   The Jackson Laboratory

Description
Mice homozygous for the Mos^tm1Ev targeted mutation are viable. Homozygous males are fertile; the littersize of homozygous females is markedly lower than that of wild type or heterozygous mice. Eggs lacking Mos undergo spontaneous parthenogenetic activation (extrusion of the second polar body and pronucleus formation without fertilization). Ovarian cysts develop in homozygous females as young as one month. Some of the ovarian cysts consist of several tissue types, including possible thyroid tissue, similar to about 10% of all benign cystic teratomas in human beings.

Development
The Mos-deficient strain was developed in the laboratory of Dr. Martin J. Evans at the University of Cambridge, UK. The targeting vector (containing the neo gene) was inserted into the middle of the single exon and introduced an amber stop codon, terminating translation of sequences essential for kinase activity. The construct was electroporated into 129S6/SvEv-derived CCE embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. Chimeric males were bred to generate heterozygotes. Heterozygotes were crossed to generate homozygotes.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls

Related Strains

Strains carrying   Mos^tm1Ev allele

002723	B6.129S6-Mostm1Ev/J
002404	B6;CBA-Mostm1Ev/J

View Strains carrying   Mos^tm1Ev     (2 strains)

Additional Web Information

New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Mos^tm1Ev/Mos^tm1Ev

        involves: 129S/SvEv

• endocrine/exocrine gland phenotype
• ovary cysts (MGI Ref ID J:19118)
  • ovarian cysts are found in 5 mutant females but not in wild-type (0/19) or heterozygous animals (0/10); incidence is high (4/7) in females older than 6 months
  • cysts are serous, bilateral, multilocular in older mice and sometimes infiltrated with blood; smaller cysts are predominantly composed of epithelial tissue, while larger cysts (2/5) contain tissue types typical of a teratoma
• reproductive system phenotype
• *normal* reproductive system phenotype (MGI Ref ID J:77797)
  • normal sperm count and spermatogenesis
• abnormal oogenesis (MGI Ref ID J:110272)
  • in Mos-deficient oocytes the meiotic spindle does not migrate in contrast to wild-type; rather, it elongates dramatically followed by cortical polarization
  • eggs isolated 18 hours after human chorionic gonadotropin treatment display 2 polar bodies and no pronuclei while egg from wild-type littermates have only one polar body
  • ~40% of mutant eggs maintained in culture develop a pronucleus indicating parthenogenetic development; 30% of these cleave into 2 nucleated cells and occasionally to 4 cells
  • mutant eggs that fail to cleave undergo cytoplasmic fragmentation which is not seen in wild-type eggs
• abnormal female meiosis (MGI Ref ID J:19118)
  • eggs failed to arrest during meiosis, leading to spontaneous parthenogenetic activation (progress through meiosis II, germinal vesicle breakdown, extrusion of polar bodies, and cleavage)
• decreased litter size (MGI Ref ID J:19118)
  • females have litters with average size of 2 pups, compared to wild-type and heterozygous females which have ~7 pups/litter
• ovary cysts (MGI Ref ID J:19118)
  • ovarian cysts are found in 5 mutant females but not in wild-type (0/19) or heterozygous animals (0/10); incidence is high (4/7) in females older than 6 months
  • cysts are serous, bilateral, multilocular in older mice and sometimes infiltrated with blood; smaller cysts are predominantly composed of epithelial tissue, while larger cysts (2/5) contain tissue types typical of a teratoma
• reduced female fertility (MGI Ref ID J:19118)
  • females but not males have reduced fertility

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Mos^tm1Ev related

Cancer Research
Oncogenes

Reproductive Biology Research
Developmental Defects Affecting Gonads (parthenogenic activation)
Fertility Defects
Gonadal Tumors

Genes & Alleles

Gene & Allele Information

Allele Symbol		Mostm1Ev
Allele Name		targeted mutation 1, Martin L Evans
Allele Type		Targeted (knock-out)
Common Name(s)		mos-/-;
Mutation Made By		M.J. Evans,   University of Cambridge
Strain of Origin		129S/SvEv-Gpi1c
ES Cell Line Name		CCE/EK.CCE
ES Cell Line Strain		129S/SvEv-Gpi1
Gene Symbol and Name		Mos, Moloney sarcoma oncogene
Chromosome		4
Gene Common Name(s)		MGC119962; MGC119963; MSV; c-mos;
Molecular Note		A neomycin resistance cassette was inserted into the middle of the single coding exon of the gene, introducing an amber stop codon into the reading frame and terminating translation upstream of sequences essential for kinase activity. [MGI Ref ID J:19118]

Genotyping

Genotyping Information

Genotyping Protocols

Mos^tm1Ev, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Colledge WH; Carlton MB; Udy GB; Evans MJ. 1994. Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs [see comments] Nature 370(6484):65-8. [PubMed: 8015609]  [MGI Ref ID J:19118]

Additional References

Mos^tm1Ev related

Dumont J; Umbhauer M; Rassinier P; Hanauer A; Verlhac MH. 2005. p90Rsk is not involved in cytostatic factor arrest in mouse oocytes. J Cell Biol 169(2):227-31. [PubMed: 15837801]  [MGI Ref ID J:98080]

Gross VS; Cooper GM. 2002. Functional analysis of sperm from c-mos-/- mice. Mol Reprod Dev 62(4):519-24. [PubMed: 12112586]  [MGI Ref ID J:77797]

Hirao Y; Eppig JJ. 1997. Analysis of the mechanism(s) of metaphase I arrest in strain LT mouse oocytes: participation of MOS. Development 124(24):5107-13. [PubMed: 9362468]  [MGI Ref ID J:45464]

Hirao Y; Eppig JJ. 1997. Parthenogenetic development of Mos-deficient mouse oocytes. Mol Reprod Dev 48(3):391-6. [PubMed: 9322252]  [MGI Ref ID J:43317]

Naz RK; Rajesh C. 2005. Gene knockouts that cause female infertility: search for novel contraceptive targets Front Biosci 10:2447-2459. [PubMed: 15970507]  [MGI Ref ID J:103183]

Oh B; Hampl A; Eppig JJ; Solter D; Knowles BB. 1998. SPIN, a substrate in the MAP kinase pathway in mouse oocytes. Mol Reprod Dev 50(2):240-9. [PubMed: 9590541]  [MGI Ref ID J:47328]

Su YQ; Wigglesworth K; Pendola FL; O'Brien MJ; Eppig JJ. 2002. Mitogen-activated protein kinase activity in cumulus cells is essential for gonadotropin-induced oocyte meiotic resumption and cumulus expansion in the mouse. Endocrinology 143(6):2221-32. [PubMed: 12021186]  [MGI Ref ID J:77527]

Verlhac MH; Kubiak JZ; Weber M; Geraud G; Colledge WH; Evans MJ; Maro B. 1996. Mos is required for MAP kinase activation and is involved in microtubule organization during meiotic maturation in the mouse. Development 122(3):815-22. [PubMed: 8631259]  [MGI Ref ID J:32974]

Verlhac MH; Lefebvre C; Guillaud P; Rassinier P; Maro B. 2000. Asymmetric division in mouse oocytes: with or without Mos. Curr Biol 10(20):1303-6. [PubMed: 11069114]  [MGI Ref ID J:110272]

Verlhac MH; Lefebvre C; Kubiak JZ; Umbhauer M; Rassinier P; Colledge W; Maro B. 2000. Mos activates MAP kinase in mouse oocytes through two opposite pathways EMBO J 19(22):6065-74. [PubMed: 11080153]  [MGI Ref ID J:65991]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Colony Maintenance

Breeding & Husbandry	Homozygous males are fertile while homozygous females have reduced fertility. When maintaining a live colony, this strain is maintained by mating heterozygous siblings. Alternatively, heterozygous females can be bred with homozygous males.
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

OncoMouse^® requires a license from DuPont, see Licenses for Strains with OncoMouse^® Technology.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

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In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.