Strain Name:

B6.129S6-Cd74tm1Liz/J

Stock Number:

002729

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S6-Iitm1Liz/J    (Changed: 01-DEC-05 )
Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S6 via EK.CCE ES cell line
 
Donating Investigator Elizabeth Bikoff,   University of Oxford.

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Iitm1Liz targeted mutation are viable and fertile. Ia-associated invariant chain deficient mice have defects in MHC Class II assembly, transport, surface expression, and antigen presentation. Homozygous mutant mice lack mature CD4+ cells.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Cd74tm1Liz allele
002730   CAn.129S6(B6)-Cd74tm1Liz/J
View Strains carrying   Cd74tm1Liz     (1 strain)

Strains carrying other alleles of Cd74
002730   CAn.129S6(B6)-Cd74tm1Liz/J
View Strains carrying other alleles of Cd74     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Cd74tm1Liz/Cd74tm1Liz

        B6.129S-Cd74tm1Liz
  • immune system phenotype
  • abnormal T-helper 1 physiology
    • in vivo, Th1 response to MOG is absent unlike in wild-type mice   (MGI Ref ID J:125654)
    • in vitro, lymph node cells from mice receiving wild-type MOG T cells exhibit reduced Th1 recall response compared with similarly treated wild-type mice   (MGI Ref ID J:125654)
    • in vivo, Th1 response to PLP is reduced compared to in wild-type mice   (MGI Ref ID J:125654)
    • in vitro, the frequency of Th1 recall response is greater than in H2-Dmatm1Luc homozygotes   (MGI Ref ID J:125654)
  • abnormal antigen presentation
    • antigen presenting cells cannot process and present recombinant MOG protein unlike wild-type cells   (MGI Ref ID J:125654)
    • abnormal antigen presentation via MHC class II
      • class II molecules are relatively ineffective for presentation of native protein antigens   (MGI Ref ID J:28966)
      • abnormal level of surface class II molecules
        • mutants expressed greatly reduced amounts of class II on spleen cells compared to wild-type   (MGI Ref ID J:28966)
      • defective assembly of class II molecules
        • in spleen cells, immature alpha and beta chains exhibiting no evidence of having been exported past the cis-Golgi are observed, whereas compact alpha/beta dimers efficiently transported past the cis-Golgi are produced by Cd74tm2Liz-deficient cells; floppy conformers are seen, compared to compact dimers in wild-type   (MGI Ref ID J:28966)
  • decreased CD4-positive T cell number
    • significant reduction in numbers of mature CD4+CD8- thymocytes in thymus and periphery is seen compared to wild-type   (MGI Ref ID J:28966)
  • decreased T cell proliferation
    • lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type   (MGI Ref ID J:28966)
  • decreased susceptibility to experimental autoimmune encephalomyelitis
    • whether MOG-primed or adoptively transferred with MOG-specific T blasts, mice fail to develop symptoms of experimental autoimmune encephalomyelitis (EAE) unlike wild-type mice   (MGI Ref ID J:125654)
    • PLP-treated mice fail to develop EAE unlike similarly treated wild-type mice   (MGI Ref ID J:125654)
  • decreased susceptibility to type IV hypersensitivity reaction
    • dramatically compared to in wild-type mice when wild-type MOG T cells are transferred   (MGI Ref ID J:125654)
  • hematopoietic system phenotype
  • decreased CD4-positive T cell number
    • significant reduction in numbers of mature CD4+CD8- thymocytes in thymus and periphery is seen compared to wild-type   (MGI Ref ID J:28966)
  • decreased T cell proliferation
    • lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type   (MGI Ref ID J:28966)
  • cellular phenotype
  • decreased T cell proliferation
    • lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type   (MGI Ref ID J:28966)

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Cd74tm1Liz/Cd74+

        involves: 129S/SvEv
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype
    • no evidence for anemia or thrombocytopenia   (MGI Ref ID J:155870)

Cd74tm1Liz/Cd74tm1Liz

        involves: 129S/SvEv * C57BL/6J
  • growth/size phenotype
  • decreased body size
    • homozygotes are usually smaller than littermates   (MGI Ref ID J:65918)
    • cachexia
      • occasionally, homozygotes display symptoms of severe wasting   (MGI Ref ID J:65918)
  • hematopoietic system phenotype
  • decreased CD4-positive T cell number
    • mutants have decreased numbers of CD4+8- TCRhi T cells in the thymus   (MGI Ref ID J:65918)
    • number of CD4+CD8- T cells in the periphery; decreased numbers of mature CD4+ T lymphocytes are seen in analyses of splenic T cells   (MGI Ref ID J:65918)
  • decreased T cell proliferation
    • immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells   (MGI Ref ID J:72243)
  • immune system phenotype
  • abnormal antigen presentation
    • splenic antigen presenting cells (APCs) present an encephalitogenic MOG peptide p35-55 to I-Ab-restricted p35-55-specific T cells equivalently to wild-type APCs, stimulating proliferation but are ineffective at presenting intact MOG to T cells compared with wild-type APCs, suggesting that processing is required for presentation of intact MOG   (MGI Ref ID J:72243)
    • abnormal antigen presentation via MHC class II
      • mutant spleen cells stimulated stronger responses in presence of already processed peptides hen egg lysozyme (HEL) 74-88 and Ealpha 52-68, but fail to present native protein antigens keyhole limpet cyanin (KLH) and IgG2a   (MGI Ref ID J:65918)
      • abnormal level of surface class II molecules
        • mutant spleen cells exhibit reduced levels of I-Ab surface antigen compared to wild-type spleen cells   (MGI Ref ID J:65918)
      • defective assembly of class II molecules
        • mutant spleen cells show significantly reduced amounts of alpha/beta heterodimers associated with the invariant chain; increased amounts of free alpha and beta chains are present   (MGI Ref ID J:65918)
        • majority of class II molecules are comprised of immature alpha and beta chains that have not been exported past the cis-Golgi   (MGI Ref ID J:65918)
        • no mature compact SDS-resistant alpha/beta heterodimers (I-Ab) are produced by mutant spleens in contrast to wild-type cells   (MGI Ref ID J:65918)
        • class II molecules produced by mutants show enhanced peptide binding compared to wild-type; molecules behave as if empty or occupied by easily displaced peptide in contrast to wild-type cells that are stably occupied with self and serum-derived peptides   (MGI Ref ID J:65918)
  • decreased CD4-positive T cell number
    • mutants have decreased numbers of CD4+8- TCRhi T cells in the thymus   (MGI Ref ID J:65918)
    • number of CD4+CD8- T cells in the periphery; decreased numbers of mature CD4+ T lymphocytes are seen in analyses of splenic T cells   (MGI Ref ID J:65918)
  • decreased T cell proliferation
    • immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells   (MGI Ref ID J:72243)
  • decreased interferon-gamma secretion
    • immunization with p35-55 does not induce Ifng secretion indicating inability to prime p35-55 specific CD4+ T cells   (MGI Ref ID J:72243)
  • decreased susceptibility to experimental autoimmune encephalomyelitis
    • mice do not develop experimental autoimmune encephalitis (EAE) upon direct immunization with p35-55 while wild-type mice do develop EAE   (MGI Ref ID J:72243)
    • adoptive transfer of wild-type encephalitogenic T cells does not induce EAE in mutant recipients   (MGI Ref ID J:72243)
  • cellular phenotype
  • decreased T cell proliferation
    • immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells   (MGI Ref ID J:72243)

Cd74tm1Liz/Cd74tm1Liz

        involves: 129S/SvEv * C57BL/6
  • immune system phenotype
  • abnormal antigen presentation via MHC class II
    • mutants display isotype-specific antigen presentation defects; MHC Ak-restricted antigen presentation is reduced compared to wild-type   (MGI Ref ID J:111565)

Cd74tm1Liz/Cd74tm1Liz

        involves: 129S/SvEv * BALB/cAn
  • immune system phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)
  • abnormal antigen presenting cell physiology
    • splenocytes display enhanced binding capabilities   (MGI Ref ID J:113714)
    • abnormal antigen presentation via MHC class II
      • mutant spleen cells on an H-2d background show enhanced peptide-loading capabilities over wild-type cells   (MGI Ref ID J:110927)
      • T cell hybridomas produce Il-2 and T cell lines proliferate when stimulated by mutant spleen cells; similar results are seen using T cell clones specific for peptides including ovalbumin (OVA)323-339 and IgG2ab   (MGI Ref ID J:110927)
      • spleen cells are ineffective for presentation of intact protein antigen as opposed to processed peptide antigens   (MGI Ref ID J:110927)
      • spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses   (MGI Ref ID J:110927)
      • antigen presentation by mutant spleen cells to Edrestricted T cell clones is substantially decreased   (MGI Ref ID J:113714)
      • Edrestricted T cells show enhanced activity when challenged with endogenously process beta 2 microglobulin (B2m) epitopes in presence of mutant splenocytes   (MGI Ref ID J:113714)
    • abnormal dendritic cell antigen presentation
      • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type   (MGI Ref ID J:110927)
  • decreased CD4-positive T cell number
    • mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold   (MGI Ref ID J:110927)
    • T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens   (MGI Ref ID J:113714)
  • hematopoietic system phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)
  • decreased CD4-positive T cell number
    • mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold   (MGI Ref ID J:110927)
    • T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens   (MGI Ref ID J:113714)
  • cellular phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)

Cd74tm1Liz/Cd74tm1Liz

        involves: 129S/SvEv * BALB/c * C57BL
  • immune system phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)
  • abnormal T cell proliferation
    • proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL46-61 peptide show similar response to wild-type mice   (MGI Ref ID J:110927)
  • abnormal antigen presentation via MHC class II
    • spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses   (MGI Ref ID J:110927)
  • abnormal dendritic cell antigen presentation
    • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type   (MGI Ref ID J:110927)
  • hematopoietic system phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)
  • abnormal T cell proliferation
    • proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL46-61 peptide show similar response to wild-type mice   (MGI Ref ID J:110927)
  • cellular phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)
  • abnormal T cell proliferation
    • proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL46-61 peptide show similar response to wild-type mice   (MGI Ref ID J:110927)

Cd74tm1Liz/Cd74tm1Liz

        involves: 129S/SvEv * C57BL * C57BL/10 * C57BR
  • immune system phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)
  • abnormal T cell proliferation
    • proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA323-339 peptide show similar response to wild-type mice   (MGI Ref ID J:110927)
  • abnormal antigen presentation via MHC class II
    • spleen cells from mice with an H-2k haplotype background effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses   (MGI Ref ID J:110927)
  • abnormal dendritic cell antigen presentation
    • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type   (MGI Ref ID J:110927)
  • hematopoietic system phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)
  • abnormal T cell proliferation
    • proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA323-339 peptide show similar response to wild-type mice   (MGI Ref ID J:110927)
  • cellular phenotype
  • abnormal B cell differentiation
    • B cell maturation is defective   (MGI Ref ID J:110927)
    • increased immature B cell number
      • higher percentages of immature B cells lacking surface IgD and CD23 expression   (MGI Ref ID J:110927)
  • abnormal T cell proliferation
    • proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA323-339 peptide show similar response to wild-type mice   (MGI Ref ID J:110927)

Cd74tm1Liz/Cd74tm1Liz

        involves: 129S/SvEv
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype
    • no evidence for anemia or thrombocytopenia   (MGI Ref ID J:155870)
    • abnormal positive T cell selection
      • CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow   (MGI Ref ID J:125548)
  • immune system phenotype
  • abnormal positive T cell selection
    • CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow   (MGI Ref ID J:125548)
  • cellular phenotype
  • abnormal positive T cell selection
    • CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow   (MGI Ref ID J:125548)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Cd74tm1Liz related

Immunology and Inflammation Research
Immunodeficiency
      MHC class II defects

Research Tools
Immunology and Inflammation Research
      MHC class II defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Cd74tm1Liz
Allele Name targeted mutation 1, Elizabeth Bikoff
Allele Type Targeted (knock-out)
Common Name(s) Ii-; MHCII-;
Strain of Origin129S/SvEv-Gpi1
ES Cell Line NameCCE/EK.CCE
ES Cell Line Strain129S/SvEv-Gpi1
Gene Symbol and Name Cd74, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Chromosome 18
Gene Common Name(s) CLIP; DHLAG; HLADG; II; INVG34; Ia-GAMMA; Ia-associated invariant chain; Ii;
Molecular Note A MC1neopA cassette replaced the first intron and 11 nucleotides of the second exon. [MGI Ref ID J:65918]

Genotyping

Genotyping Information

Genotyping Protocols

Cd74tm1Liz, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Bikoff EK; Huang LY; Episkopou V; van Meerwijk J; Germain RN; Robertson EJ. 1993. Defective major histocompatibility complex class II assembly, transport, peptide acquisition, and CD4+ T cell selection in mice lacking invariant chain expression. J Exp Med 177(6):1699-712. [PubMed: 8098731]  [MGI Ref ID J:65918]

Additional References

Elewaut D; Lawton AP; Nagarajan NA; Maverakis E; Khurana A; Honing S; Benedict CA; Sercarz E; Bakke O; Kronenberg M; Prigozy TI. 2003. The adaptor protein AP-3 is required for CD1d-mediated antigen presentation of glycosphingolipids and development of Valpha14i NKT cells. J Exp Med 198(8):1133-46. [PubMed: 14557411]  [MGI Ref ID J:86243]

Fan L; Busser BW; Lifsted TQ; Lo D; Laufer TM. 2003. Antigen presentation by keratinocytes directs autoimmune skin disease. Proc Natl Acad Sci U S A 100(6):3386-91. [PubMed: 12629221]  [MGI Ref ID J:82725]

Honey K; Forbush K; Jensen PE; Rudensky AY. 2004. Effect of decreasing the affinity of the class II-associated invariant chain peptide on the MHC class II peptide repertoire in the presence or absence of H-2M. J Immunol 172(7):4142-50. [PubMed: 15034026]  [MGI Ref ID J:88717]

Shen L; Sigal LJ; Boes M; Rock KL. 2004. Important role of cathepsin S in generating peptides for TAP-independent MHC class I crosspresentation in vivo. Immunity 21(2):155-65. [PubMed: 15308097]  [MGI Ref ID J:93590]

Cd74tm1Liz related

Alard P; Clark SL; Kosiewicz MM. 2004. Mechanisms of tolerance induced by TGF beta-treated APC: CD4 regulatory T cells prevent the induction of the immune response possibly through a mechanism involving TGF beta. Eur J Immunol 34(4):1021-30. [PubMed: 15048712]  [MGI Ref ID J:115475]

Barlow JL; Drynan LF; Hewett DR; Holmes LR; Lorenzo-Abalde S; Lane AL; Jolin HE; Pannell R; Middleton AJ; Wong SH; Warren AJ; Wainscoat JS; Boultwood J; McKenzie AN. 2010. A p53-dependent mechanism underlies macrocytic anemia in a mouse model of human 5q- syndrome. Nat Med 16(1):59-66. [PubMed: 19966810]  [MGI Ref ID J:155870]

Barton GM; Beers C; deRoos P; Eastman SR; Gomez ME; Forbush KA; Rudensky AY. 2002. Positive selection of self-MHC-reactive T cells by individual peptide-MHC class II complexes. Proc Natl Acad Sci U S A 99(10):6937-42. [PubMed: 12011451]  [MGI Ref ID J:135481]

Barton GM; Rudensky AY. 1998. An altered invariant chain protein with an antigenic peptide in place of CLIP forms SDS-stable complexes with class II alphabeta dimers and facilitates highly efficient peptide loading. Int Immunol 10(8):1159-65. [PubMed: 9723702]  [MGI Ref ID J:136433]

Barton GM; Rudensky AY. 1999. Requirement for diverse, low-abundance peptides in positive selection of T cells. Science 283(5398):67-70. [PubMed: 9872742]  [MGI Ref ID J:77662]

Bikoff EK; Germain RN; Robertson EJ. 1995. Allelic differences affecting invariant chain dependency of MHC class II subunit assembly. Immunity 2(3):301-10. [PubMed: 7697546]  [MGI Ref ID J:109917]

Bikoff EK; Wutz G; Kenty GA; Koonce CH; Robertson EJ. 2001. Relaxed DM requirements during class II peptide loading and CD4+ T cell maturation in BALB/c mice. J Immunol 166(8):5087-98. [PubMed: 11290790]  [MGI Ref ID J:113714]

Cady CT; Lahn M; Vollmer M; Tsuji M; Seo SJ; Reardon CL; O'Brien RL; Born WK. 2000. Response of murine gamma delta T cells to the synthetic polypeptide poly-Glu50Tyr50. J Immunol 165(4):1790-8. [PubMed: 10925256]  [MGI Ref ID J:120419]

Fan L; Busser BW; Lifsted TQ; Lo D; Laufer TM. 2003. Antigen presentation by keratinocytes directs autoimmune skin disease. Proc Natl Acad Sci U S A 100(6):3386-91. [PubMed: 12629221]  [MGI Ref ID J:82725]

Grubin CE; Kovats S; deRoos P; Rudensky AY. 1997. Deficient positive selection of CD4 T cells in mice displaying altered repertoires of MHC class II-bound self-peptides. Immunity 7(2):197-208. [PubMed: 9285405]  [MGI Ref ID J:84193]

Honey K; Forbush K; Jensen PE; Rudensky AY. 2004. Effect of decreasing the affinity of the class II-associated invariant chain peptide on the MHC class II peptide repertoire in the presence or absence of H-2M. J Immunol 172(7):4142-50. [PubMed: 15034026]  [MGI Ref ID J:88717]

Honey K; Nakagawa T; Peters C; Rudensky A. 2002. Cathepsin L regulates CD4+ T cell selection independently of its effect on invariant chain: a role in the generation of positively selecting peptide ligands. J Exp Med 195(10):1349-58. [PubMed: 12021314]  [MGI Ref ID J:112070]

Huang LY; van Meerwijk JP; Bikoff EK; Germain RN. 1996. Comparison of thymocyte development in normal and invariant chain-deficient mice provides evidence that maturation-related changes in TCR and co-receptor levels play a critical role in cell fate. Int Immunol 8(9):1429-40. [PubMed: 8921421]  [MGI Ref ID J:35531]

Ignatowicz L; Kappler J; Marrack P. 1996. The repertoire of T cells shaped by a single MHC/peptide ligand. Cell 84(4):521-9. [PubMed: 8598039]  [MGI Ref ID J:58036]

Kamala T; Nanda NK. 2009. Protective response to Leishmania major in BALB/c mice requires antigen processing in the absence of DM. J Immunol 182(8):4882-90. [PubMed: 19342667]  [MGI Ref ID J:147504]

Kenty G; Bikoff EK. 1999. BALB/c invariant chain mutant mice display relatively efficient maturation of CD4+ T cells in the periphery and secondary proliferative responses elicited upon peptide challenge. J Immunol 163(1):232-41. [PubMed: 10384121]  [MGI Ref ID J:110927]

Kenty G; Martin WD; Van Kaer L; Bikoff EK. 1998. MHC class II expression in double mutant mice lacking invariant chain and DM functions. J Immunol 160(2):606-14. [PubMed: 9551894]  [MGI Ref ID J:45191]

Koonce CH; Bikoff EK. 2004. Dissecting MHC class II export, B cell maturation, and DM stability defects in invariant chain mutant mice. J Immunol 173(5):3271-80. [PubMed: 15322189]  [MGI Ref ID J:92720]

Koonce CH; Wutz G; Robertson EJ; Vogt AB; Kropshofer H; Bikoff EK. 2003. DM loss in k haplotype mice reveals isotype-specific chaperone requirements. J Immunol 170(7):3751-61. [PubMed: 12646641]  [MGI Ref ID J:111565]

Kosiewicz MM; Alard P; Liang S; Clark SL. 2004. Mechanisms of tolerance induced by transforming growth factor-beta-treated antigen-presenting cells: CD8 regulatory T cells inhibit the effector phase of the immune response in primed mice through a mechanism involving Fas ligand. Int Immunol 16(5):697-706. [PubMed: 15096489]  [MGI Ref ID J:89454]

Kovats S; Grubin CE; Eastman S; deRoos P; Dongre A; Van Kaer L; Rudensky AY. 1998. Invariant chain-independent function of H-2M in the formation of endogenous peptide-major histocompatibility complex class II complexes in vivo. J Exp Med 187(2):245-51. [PubMed: 9432982]  [MGI Ref ID J:88532]

Kraj P; Pacholczyk R; Ignatowicz L. 2001. Alpha beta TCRs differ in the degree of their specificity for the positively selecting MHC/peptide ligand. J Immunol 166(4):2251-9. [PubMed: 11160279]  [MGI Ref ID J:126970]

Lau AW; Biester S; Cornall RJ; Forrester JV. 2008. Lipopolysaccharide-Activated IL-10-Secreting Dendritic Cells Suppress Experimental Autoimmune Uveoretinitis by MHCII-Dependent Activation of CD62L-Expressing Regulatory T Cells. J Immunol 180(6):3889-99. [PubMed: 18322197]  [MGI Ref ID J:133030]

Lennon-Dumenil AM; Roberts RA; Valentijn K; Driessen C; Overkleeft HS; Erickson A; Peters PJ; Bikoff E; Ploegh HL; Wolf Bryant P. 2001. The p41 isoform of invariant chain is a chaperone for cathepsin L. EMBO J 20(15):4055-64. [PubMed: 11483509]  [MGI Ref ID J:115604]

Logunova NN; Viret C; Pobezinsky LA; Miller SA; Kazansky DB; Sundberg JP; Chervonsky AV. 2005. Restricted MHC-peptide repertoire predisposes to autoimmunity. J Exp Med 202(1):73-84. [PubMed: 15998789]  [MGI Ref ID J:100625]

Maehr R; Kraus M; Ploegh HL. 2004. Mice deficient in invariant-chain and MHC class II exhibit a normal mature B2 cell compartment. Eur J Immunol 34(8):2230-6. [PubMed: 15259020]  [MGI Ref ID J:91771]

Matza D; Lantner F; Bogoch Y; Flaishon L; Hershkoviz R; Shachar I. 2002. Invariant chain induces B cell maturation in a process that is independent of its chaperonic activity. Proc Natl Acad Sci U S A 99(5):3018-23. [PubMed: 11867743]  [MGI Ref ID J:126904]

Ness TL; Ewing JL; Hogaboam CM; Kunkel SL. 2006. CCR4 is a key modulator of innate immune responses. J Immunol 177(11):7531-9. [PubMed: 17114422]  [MGI Ref ID J:140604]

Pacholczyk R; Kern J; Singh N; Iwashima M; Kraj P; Ignatowicz L. 2007. Nonself-antigens are the cognate specificities of Foxp3+ regulatory T cells. Immunity 27(3):493-504. [PubMed: 17869133]  [MGI Ref ID J:125322]

Pacholczyk R; Kraj P; Ignatowicz L. 2002. Peptide specificity of thymic selection of CD4+CD25+ T cells. J Immunol 168(2):613-20. [PubMed: 11777953]  [MGI Ref ID J:126012]

Salek-Ardakani S; Flynn R; Arens R; Yagita H; Smith GL; Borst J; Schoenberger SP; Croft M. 2011. The TNFR family members OX40 and CD27 link viral virulence to protective T cell vaccines in mice. J Clin Invest 121(1):296-307. [PubMed: 21183789]  [MGI Ref ID J:171838]

Slavin AJ; Soos JM; Stuve O; Patarroyo JC; Weiner HL; Fontana A; Bikoff EK; Zamvil SS. 2001. Requirement for endocytic antigen processing and influence of invariant chain and H-2M deficiencies in CNS autoimmunity. J Clin Invest 108(8):1133-9. [PubMed: 11602620]  [MGI Ref ID J:72243]

Sun J; Hartvigsen K; Chou MY; Zhang Y; Sukhova GK; Zhang J; Lopez-Ilasaca M; Diehl CJ; Yakov N; Harats D; George J; Witztum JL; Libby P; Ploegh H; Shi GP. 2010. Deficiency of antigen-presenting cell invariant chain reduces atherosclerosis in mice. Circulation 122(8):808-20. [PubMed: 20697023]  [MGI Ref ID J:178588]

Takaesu NT; Lower JA; Robertson EJ; Bikoff EK. 1995. Major histocompatibility class II peptide occupancy, antigen presentation, and CD4+ T cell function in mice lacking the p41 isoform of invariant chain. Immunity 3(3):385-96. [PubMed: 7553002]  [MGI Ref ID J:28966]

Takaesu NT; Lower JA; Yelon D; Robertson EJ; Bikoff EK. 1997. In vivo functions mediated by the p41 isoform of the MHC class II-associated invariant chain. J Immunol 158(1):187-99. [PubMed: 8977190]  [MGI Ref ID J:82880]

Tompkins SM; Padilla J; Dal Canto MC; Ting JP; Van Kaer L; Miller SD. 2002. De novo central nervous system processing of myelin antigen is required for the initiation of experimental autoimmune encephalomyelitis. J Immunol 168(8):4173-83. [PubMed: 11937578]  [MGI Ref ID J:125654]

Wong P; Goldrath AW; Rudensky AY. 2000. Competition for specific intrathymic ligands limits positive selection in a TCR transgenic model of CD4+ T cell development. J Immunol 164(12):6252-9. [PubMed: 10843678]  [MGI Ref ID J:125548]

Ye Q; Finn PW; Sweeney R; Bikoff EK; Riese RJ. 2003. MHC class II-associated invariant chain isoforms regulate pulmonary immune responses. J Immunol 170(3):1473-80. [PubMed: 12538710]  [MGI Ref ID J:126977]

Zielinski CE; Jacob SN; Bouzahzah F; Ehrlich BE; Craft J. 2005. Naive CD4+ T cells from lupus-prone Fas-intact MRL mice display TCR-mediated hyperproliferation due to intrinsic threshold defects in activation. J Immunol 174(8):5100-9. [PubMed: 15814741]  [MGI Ref ID J:98149]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, RG10/RG30.

Purchasing information

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing
Order this mouse

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $1980.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $1600.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing
Order this mouse

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2574.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Embryos

Price (US dollars $)
Frozen Embryo $2080.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

General Supply Notes

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries

Contracts Administration

phone:207-288-6470
fax:207-288-6655

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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