Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S6-Iitm1Liz/J    (Changed: 01-DEC-05 ) Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6 Donor Strain 129S6 via EK.CCE ES cell line   Donating Investigator Elizabeth Bikoff,   University of Oxford.

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Ii^tm1Liz targeted mutation are viable and fertile. Ia-associated invariant chain deficient mice have defects in MHC Class II assembly, transport, surface expression, and antigen presentation. Homozygous mutant mice lack mature CD4⁺ cells.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Cd74^tm1Liz allele

002730

CAn.129S6(B6)-Cd74tm1Liz/J

View Strains carrying   Cd74^tm1Liz     (1 strain)

Strains carrying other alleles of Cd74

008224

NOD.129S2(B6)-Cd74tm1Doi/LwnJ

View Strains carrying other alleles of Cd74     (1 strain)

Additional Web Information

Congenic Nomenclature

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Cd74^tm1Liz/Cd74^tm1Liz

        B6.129S-Cd74^tm1Liz

• immune system phenotype
• abnormal antigen presentation via MHC class II (MGI Ref ID J:28966)
  • class II molecules are relatively ineffective for presentation of native protein antigens
• abnormal level of surface class II molecules (MGI Ref ID J:28966)
  • mutants expressed greatly reduced amounts of class II on spleen cells compared to wild-type
• defective assembly of class II molecules (MGI Ref ID J:28966)
  • in spleen cells, immature alpha and beta chains exhibiting no evidence of having been exported past the cis-Golgi are observed, whereas compact alpha/beta dimers efficiently transported past the cis-Golgi are produced by Cd74^tm2Liz-deficient cells; floppy conformers are seen, compared to compact dimers in wild-type
• decreased CD4-positive T cell number (MGI Ref ID J:28966)
  • significant reduction in numbers of mature CD4+CD8- thymocytes in thymus and periphery is seen compared to wild-type
• decreased T cell proliferation (MGI Ref ID J:28966)
  • lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type
• hematopoietic system phenotype
• decreased CD4-positive T cell number (MGI Ref ID J:28966)
  • significant reduction in numbers of mature CD4+CD8- thymocytes in thymus and periphery is seen compared to wild-type
• decreased T cell proliferation (MGI Ref ID J:28966)
  • lymph node cells show significantly lower proliferative responses to immunization with keyhole limpet cyanin (KLH) compared to wild-type

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Cd74^tm1Liz/Cd74^tm1Liz

        involves: 129S/SvEv * C57BL/6J

• growth/size phenotype
• decreased body size (MGI Ref ID J:65918)
  • homozygotes are usually smaller than littermates
• cachexia (MGI Ref ID J:65918)
  • occasionally, homozygotes display symptoms of severe wasting
• hematopoietic system phenotype
• decreased CD4-positive T cell number (MGI Ref ID J:65918)
  • mutants have decreased numbers of CD4+8- TCRhi T cells in the thymus
  • number of CD4+CD8- T cells in the periphery; decreased numbers of mature CD4+ T lymphocytes are seen in analyses of splenic T cells
• decreased T cell proliferation (MGI Ref ID J:72243)
  • immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells
• immune system phenotype
• abnormal antigen presentation (MGI Ref ID J:72243)
  • splenic antigen presenting cells (APCs) present an encephalitogenic MOG peptide p35-55 to I-A^b-restricted p35-55-specific T cells equivalently to wild-type APCs, stimulating proliferation but are ineffective at presenting intact MOG to T cells compared with wild-type APCs, suggesting that processing is required for presentation of intact MOG
• abnormal antigen presentation via MHC class II (MGI Ref ID J:65918)
  • mutant spleen cells stimulated stronger responses in presence of already processed peptides hen egg lysozyme (HEL) 74-88 and Ealpha 52-68, but fail to present native protein antigens keyhole limpet cyanin (KLH) and IgG2a
• abnormal level of surface class II molecules (MGI Ref ID J:65918)
  • mutant spleen cells exhibit reduced levels of I-A^b surface antigen compared to wild-type spleen cells
• defective assembly of class II molecules (MGI Ref ID J:65918)
  • mutant spleen cells show significantly reduced amounts of alpha/beta heterodimers associated with the invariant chain; increased amounts of free alpha and beta chains are present
  • majority of class II molecules are comprised of immature alpha and beta chains that have not been exported past the cis-Golgi
  • no mature compact SDS-resistant alpha/beta heterodimers (I-A^b) are produced by mutant spleens in contrast to wild-type cells
  • class II molecules produced by mutants show enhanced peptide binding compared to wild-type; molecules behave as if empty or occupied by easily displaced peptide in contrast to wild-type cells that are stably occupied with self and serum-derived peptides
• decreased CD4-positive T cell number (MGI Ref ID J:65918)
  • mutants have decreased numbers of CD4+8- TCRhi T cells in the thymus
  • number of CD4+CD8- T cells in the periphery; decreased numbers of mature CD4+ T lymphocytes are seen in analyses of splenic T cells
• decreased T cell proliferation (MGI Ref ID J:72243)
  • immunization with p35-55 does not induce significant T cell proliferation compared to wild-type cells
• decreased interferon-gamma secretion (MGI Ref ID J:72243)
  • immunization with p35-55 does not induce Ifng secretion indicating inability to prime p35-55 specific CD4+ T cells
• decreased susceptibility to experimental autoimmune encephalomyelitis (MGI Ref ID J:72243)
  • mice do not develop experimental autoimmune encephalitis (EAE) upon direct immunization with p35-55 while wild-type mice do develop EAE
  • adoptive transfer of wild-type encephalitogenic T cells does not induce EAE in mutant recipients

Cd74^tm1Liz/Cd74^tm1Liz

        involves: 129S/SvEv * C57BL/6

• immune system phenotype
• abnormal antigen presentation via MHC class II (MGI Ref ID J:111565)
  • mutants display isotype-specific antigen presentation defects; MHC A^k-restricted antigen presentation is reduced compared to wild-type

Cd74^tm1Liz/Cd74^tm1Liz

        involves: 129S/SvEv * BALB/cAn

• immune system phenotype
• abnormal B cell differentiation (MGI Ref ID J:110927)
  • B cell maturation is defective
• increased immature B cell number (MGI Ref ID J:110927)
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
• abnormal antigen presenting cell physiology (MGI Ref ID J:113714)
  • splenocytes display enhanced binding capabilities
• abnormal antigen presentation via MHC class II (MGI Ref ID J:110927)
  • mutant spleen cells on an H-2^d background show enhanced peptide-loading capabilities over wild-type cells
  • T cell hybridomas produce Il-2 and T cell lines proliferate when stimulated by mutant spleen cells; similar results are seen using T cell clones specific for peptides including ovalbumin (OVA)_323-339 and IgG2a^b
  • spleen cells are ineffective for presentation of intact protein antigen as opposed to processed peptide antigens
  • spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses
  • antigen presentation by mutant spleen cells to E^drestricted T cell clones is substantially decreased
  • E^drestricted T cells show enhanced activity when challenged with endogenously process beta 2 microglobulin (B2m) epitopes in presence of mutant splenocytes
• abnormal dendritic cell antigen presentation (MGI Ref ID J:110927)
  • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
• decreased CD4-positive T cell number (MGI Ref ID J:110927)
  • mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold
  • T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens
• hematopoietic system phenotype
• abnormal B cell differentiation (MGI Ref ID J:110927)
  • B cell maturation is defective
• increased immature B cell number (MGI Ref ID J:110927)
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
• decreased CD4-positive T cell number (MGI Ref ID J:110927)
  • mutant thymi display 3-fold fewer mature CD4+ T cells compared to wild-type; spleen and lymph node CD4+ T cell percentages are reduced ~2-fold
  • T cells carrying Vbeta (VB) segments (VB3, 5, 11 and 12) are well represented whereas they are completely eliminated in wild-type BALB/c controls, suggesting a role during presentation of endogenous superantigens

Cd74^tm1Liz/Cd74^tm1Liz

        involves: 129S/SvEv * BALB/c * C57BL

• immune system phenotype
• abnormal B cell differentiation (MGI Ref ID J:110927)
  • B cell maturation is defective
• increased immature B cell number (MGI Ref ID J:110927)
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
• abnormal T cell proliferation (MGI Ref ID J:110927)
  • proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL_46-61 peptide show similar response to wild-type mice
• abnormal antigen presentation via MHC class II (MGI Ref ID J:110927)
  • spleen cells effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses
• abnormal dendritic cell antigen presentation (MGI Ref ID J:110927)
  • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
• hematopoietic system phenotype
• abnormal B cell differentiation (MGI Ref ID J:110927)
  • B cell maturation is defective
• increased immature B cell number (MGI Ref ID J:110927)
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
• abnormal T cell proliferation (MGI Ref ID J:110927)
  • proliferative responses to intact hen egg lysozyme (HEL) protein antigen are reduced in mutants, but immunized mice challenged secondarily with HEL_46-61 peptide show similar response to wild-type mice

Cd74^tm1Liz/Cd74^tm1Liz

        involves: 129S/SvEv * C57BL * C57BL/10 * C57BR

• immune system phenotype
• abnormal B cell differentiation (MGI Ref ID J:110927)
  • B cell maturation is defective
• increased immature B cell number (MGI Ref ID J:110927)
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
• abnormal T cell proliferation (MGI Ref ID J:110927)
  • proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA_323-339 peptide show similar response to wild-type mice
• abnormal antigen presentation via MHC class II (MGI Ref ID J:110927)
  • spleen cells from mice with an H-2^k haplotype background effectively function as stimulators for allogeneic T cells, eliciting strong mixed lymphocyte responses
• abnormal dendritic cell antigen presentation (MGI Ref ID J:110927)
  • dendritic cells show reduced class II surface expression in overnight culture compared to wild-type
• hematopoietic system phenotype
• abnormal B cell differentiation (MGI Ref ID J:110927)
  • B cell maturation is defective
• increased immature B cell number (MGI Ref ID J:110927)
  • higher percentages of immature B cells lacking surface IgD and CD23 expression
• abnormal T cell proliferation (MGI Ref ID J:110927)
  • proliferative responses to intact protein antigens are reduced in mutants, but immunized mice challenged secondarily with OVA_323-339 peptide show similar response to wild-type mice

Cd74^tm1Liz/Cd74^tm1Liz

        involves: 129S/SvEv

• hematopoietic system phenotype
• abnormal positive T cell selection (MGI Ref ID J:125548)
  • CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow
• immune system phenotype
• abnormal positive T cell selection (MGI Ref ID J:125548)
  • CD4+ T cell-selection is almost completely abrogated in lethally irradiated hosts upon transfer of transgenic bone marrow

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Cd74^tm1Liz related

Immunology and Inflammation Research
Immunodeficiency (MHC class II defects)

Research Tools
Immunology and Inflammation Research (MHC class II defects)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Cd74tm1Liz
Allele Name		targeted mutation 1, Elizabeth Bikoff
Allele Type		Targeted (knock-out)
Common Name(s)		Ii-; MHCII-;
Strain of Origin		129S/SvEv-Gpi1
ES Cell Line Name		CCE/EK.CCE
ES Cell Line Strain		129S/SvEv-Gpi1
Gene Symbol and Name		Cd74, CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)
Chromosome		18
Gene Common Name(s)		CLIP; DHLAG; HLA-DR-GAMMA; HLADG; INVG34; Ia-GAMMA; Ia-associated invariant chain; Ii;
Molecular Note		A MC1neopA cassette replaced the first intron and 11 nucleotides of the second exon. [MGI Ref ID J:65918]

Genotyping

Genotyping Information

Genotyping Protocols

Cd74^tm1Liz, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Bikoff EK; Huang LY; Episkopou V; van Meerwijk J; Germain RN; Robertson EJ. 1993. Defective major histocompatibility complex class II assembly, transport, peptide acquisition, and CD4+ T cell selection in mice lacking invariant chain expression. J Exp Med 177(6):1699-712. [PubMed: 8098731]  [MGI Ref ID J:65918]

Additional References

Elewaut D; Lawton AP; Nagarajan NA; Maverakis E; Khurana A; Honing S; Benedict CA; Sercarz E; Bakke O; Kronenberg M; Prigozy TI. 2003. The adaptor protein AP-3 is required for CD1d-mediated antigen presentation of glycosphingolipids and development of Valpha14i NKT cells. J Exp Med 198(8):1133-46. [PubMed: 14557411]  [MGI Ref ID J:86243]

Fan L; Busser BW; Lifsted TQ; Lo D; Laufer TM. 2003. Antigen presentation by keratinocytes directs autoimmune skin disease. Proc Natl Acad Sci U S A 100(6):3386-91. [PubMed: 12629221]  [MGI Ref ID J:82725]

Honey K; Forbush K; Jensen PE; Rudensky AY. 2004. Effect of decreasing the affinity of the class II-associated invariant chain peptide on the MHC class II peptide repertoire in the presence or absence of H-2M. J Immunol 172(7):4142-50. [PubMed: 15034026]  [MGI Ref ID J:88717]

Shen L; Sigal LJ; Boes M; Rock KL. 2004. Important role of cathepsin S in generating peptides for TAP-independent MHC class I crosspresentation in vivo. Immunity 21(2):155-65. [PubMed: 15308097]  [MGI Ref ID J:93590]

Cd74^tm1Liz related

Alard P; Clark SL; Kosiewicz MM. 2004. Mechanisms of tolerance induced by TGF beta-treated APC: CD4 regulatory T cells prevent the induction of the immune response possibly through a mechanism involving TGF beta. Eur J Immunol 34(4):1021-30. [PubMed: 15048712]  [MGI Ref ID J:115475]

Barton GM; Beers C; deRoos P; Eastman SR; Gomez ME; Forbush KA; Rudensky AY. 2002. Positive selection of self-MHC-reactive T cells by individual peptide-MHC class II complexes. Proc Natl Acad Sci U S A 99(10):6937-42. [PubMed: 12011451]  [MGI Ref ID J:135481]

Barton GM; Rudensky AY. 1998. An altered invariant chain protein with an antigenic peptide in place of CLIP forms SDS-stable complexes with class II alphabeta dimers and facilitates highly efficient peptide loading. Int Immunol 10(8):1159-65. [PubMed: 9723702]  [MGI Ref ID J:136433]

Barton GM; Rudensky AY. 1999. Requirement for diverse, low-abundance peptides in positive selection of T cells. Science 283(5398):67-70. [PubMed: 9872742]  [MGI Ref ID J:77662]

Bikoff EK; Germain RN; Robertson EJ. 1995. Allelic differences affecting invariant chain dependency of MHC class II subunit assembly. Immunity 2(3):301-10. [PubMed: 7697546]  [MGI Ref ID J:109917]

Bikoff EK; Wutz G; Kenty GA; Koonce CH; Robertson EJ. 2001. Relaxed DM requirements during class II peptide loading and CD4+ T cell maturation in BALB/c mice. J Immunol 166(8):5087-98. [PubMed: 11290790]  [MGI Ref ID J:113714]

Cady CT; Lahn M; Vollmer M; Tsuji M; Seo SJ; Reardon CL; O'Brien RL; Born WK. 2000. Response of murine gamma delta T cells to the synthetic polypeptide poly-Glu50Tyr50. J Immunol 165(4):1790-8. [PubMed: 10925256]  [MGI Ref ID J:120419]

Fan L; Busser BW; Lifsted TQ; Lo D; Laufer TM. 2003. Antigen presentation by keratinocytes directs autoimmune skin disease. Proc Natl Acad Sci U S A 100(6):3386-91. [PubMed: 12629221]  [MGI Ref ID J:82725]

Grubin CE; Kovats S; deRoos P; Rudensky AY. 1997. Deficient positive selection of CD4 T cells in mice displaying altered repertoires of MHC class II-bound self-peptides. Immunity 7(2):197-208. [PubMed: 9285405]  [MGI Ref ID J:84193]

Honey K; Forbush K; Jensen PE; Rudensky AY. 2004. Effect of decreasing the affinity of the class II-associated invariant chain peptide on the MHC class II peptide repertoire in the presence or absence of H-2M. J Immunol 172(7):4142-50. [PubMed: 15034026]  [MGI Ref ID J:88717]

Honey K; Nakagawa T; Peters C; Rudensky A. 2002. Cathepsin L regulates CD4+ T cell selection independently of its effect on invariant chain: a role in the generation of positively selecting peptide ligands. J Exp Med 195(10):1349-58. [PubMed: 12021314]  [MGI Ref ID J:112070]

Huang LY; van Meerwijk JP; Bikoff EK; Germain RN. 1996. Comparison of thymocyte development in normal and invariant chain-deficient mice provides evidence that maturation-related changes in TCR and co-receptor levels play a critical role in cell fate. Int Immunol 8(9):1429-40. [PubMed: 8921421]  [MGI Ref ID J:35531]

Ignatowicz L; Kappler J; Marrack P. 1996. The repertoire of T cells shaped by a single MHC/peptide ligand. Cell 84(4):521-9. [PubMed: 8598039]  [MGI Ref ID J:58036]

Kenty G; Bikoff EK. 1999. BALB/c invariant chain mutant mice display relatively efficient maturation of CD4+ T cells in the periphery and secondary proliferative responses elicited upon peptide challenge. J Immunol 163(1):232-41. [PubMed: 10384121]  [MGI Ref ID J:110927]

Kenty G; Martin WD; Van Kaer L; Bikoff EK. 1998. MHC class II expression in double mutant mice lacking invariant chain and DM functions. J Immunol 160(2):606-14. [PubMed: 9551894]  [MGI Ref ID J:45191]

Koonce CH; Bikoff EK. 2004. Dissecting MHC class II export, B cell maturation, and DM stability defects in invariant chain mutant mice. J Immunol 173(5):3271-80. [PubMed: 15322189]  [MGI Ref ID J:92720]

Koonce CH; Wutz G; Robertson EJ; Vogt AB; Kropshofer H; Bikoff EK. 2003. DM loss in k haplotype mice reveals isotype-specific chaperone requirements. J Immunol 170(7):3751-61. [PubMed: 12646641]  [MGI Ref ID J:111565]

Kosiewicz MM; Alard P; Liang S; Clark SL. 2004. Mechanisms of tolerance induced by transforming growth factor-beta-treated antigen-presenting cells: CD8 regulatory T cells inhibit the effector phase of the immune response in primed mice through a mechanism involving Fas ligand. Int Immunol 16(5):697-706. [PubMed: 15096489]  [MGI Ref ID J:89454]

Kovats S; Grubin CE; Eastman S; deRoos P; Dongre A; Van Kaer L; Rudensky AY. 1998. Invariant chain-independent function of H-2M in the formation of endogenous peptide-major histocompatibility complex class II complexes in vivo. J Exp Med 187(2):245-51. [PubMed: 9432982]  [MGI Ref ID J:88532]

Kraj P; Pacholczyk R; Ignatowicz L. 2001. Alpha beta TCRs differ in the degree of their specificity for the positively selecting MHC/peptide ligand. J Immunol 166(4):2251-9. [PubMed: 11160279]  [MGI Ref ID J:126970]

Lau AW; Biester S; Cornall RJ; Forrester JV. 2008. Lipopolysaccharide-Activated IL-10-Secreting Dendritic Cells Suppress Experimental Autoimmune Uveoretinitis by MHCII-Dependent Activation of CD62L-Expressing Regulatory T Cells. J Immunol 180(6):3889-99. [PubMed: 18322197]  [MGI Ref ID J:133030]

Lennon-Dumenil AM; Roberts RA; Valentijn K; Driessen C; Overkleeft HS; Erickson A; Peters PJ; Bikoff E; Ploegh HL; Wolf Bryant P. 2001. The p41 isoform of invariant chain is a chaperone for cathepsin L. EMBO J 20(15):4055-64. [PubMed: 11483509]  [MGI Ref ID J:115604]

Logunova NN; Viret C; Pobezinsky LA; Miller SA; Kazansky DB; Sundberg JP; Chervonsky AV. 2005. Restricted MHC-peptide repertoire predisposes to autoimmunity. J Exp Med 202(1):73-84. [PubMed: 15998789]  [MGI Ref ID J:100625]

Maehr R; Kraus M; Ploegh HL. 2004. Mice deficient in invariant-chain and MHC class II exhibit a normal mature B2 cell compartment. Eur J Immunol 34(8):2230-6. [PubMed: 15259020]  [MGI Ref ID J:91771]

Matza D; Lantner F; Bogoch Y; Flaishon L; Hershkoviz R; Shachar I. 2002. Invariant chain induces B cell maturation in a process that is independent of its chaperonic activity. Proc Natl Acad Sci U S A 99(5):3018-23. [PubMed: 11867743]  [MGI Ref ID J:126904]

Ness TL; Ewing JL; Hogaboam CM; Kunkel SL. 2006. CCR4 is a key modulator of innate immune responses. J Immunol 177(11):7531-9. [PubMed: 17114422]  [MGI Ref ID J:140604]

Pacholczyk R; Kern J; Singh N; Iwashima M; Kraj P; Ignatowicz L. 2007. Nonself-antigens are the cognate specificities of Foxp3+ regulatory T cells. Immunity 27(3):493-504. [PubMed: 17869133]  [MGI Ref ID J:125322]

Pacholczyk R; Kraj P; Ignatowicz L. 2002. Peptide specificity of thymic selection of CD4+CD25+ T cells. J Immunol 168(2):613-20. [PubMed: 11777953]  [MGI Ref ID J:126012]

Slavin AJ; Soos JM; Stuve O; Patarroyo JC; Weiner HL; Fontana A; Bikoff EK; Zamvil SS. 2001. Requirement for endocytic antigen processing and influence of invariant chain and H-2M deficiencies in CNS autoimmunity. J Clin Invest 108(8):1133-9. [PubMed: 11602620]  [MGI Ref ID J:72243]

Takaesu NT; Lower JA; Robertson EJ; Bikoff EK. 1995. Major histocompatibility class II peptide occupancy, antigen presentation, and CD4+ T cell function in mice lacking the p41 isoform of invariant chain. Immunity 3(3):385-96. [PubMed: 7553002]  [MGI Ref ID J:28966]

Takaesu NT; Lower JA; Yelon D; Robertson EJ; Bikoff EK. 1997. In vivo functions mediated by the p41 isoform of the MHC class II-associated invariant chain. J Immunol 158(1):187-99. [PubMed: 8977190]  [MGI Ref ID J:82880]

Tompkins SM; Padilla J; Dal Canto MC; Ting JP; Van Kaer L; Miller SD. 2002. De novo central nervous system processing of myelin antigen is required for the initiation of experimental autoimmune encephalomyelitis. J Immunol 168(8):4173-83. [PubMed: 11937578]  [MGI Ref ID J:125654]

Wong P; Goldrath AW; Rudensky AY. 2000. Competition for specific intrathymic ligands limits positive selection in a TCR transgenic model of CD4+ T cell development. J Immunol 164(12):6252-9. [PubMed: 10843678]  [MGI Ref ID J:125548]

Ye Q; Finn PW; Sweeney R; Bikoff EK; Riese RJ. 2003. MHC class II-associated invariant chain isoforms regulate pulmonary immune responses. J Immunol 170(3):1473-80. [PubMed: 12538710]  [MGI Ref ID J:126977]

Zielinski CE; Jacob SN; Bouzahzah F; Ehrlich BE; Craft J. 2005. Naive CD4+ T cells from lupus-prone Fas-intact MRL mice display TCR-mediated hyperproliferation due to intrinsic threshold defects in activation. J Immunol 174(8):5100-9. [PubMed: 15814741]  [MGI Ref ID J:98149]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryopreserved Embryos This strain is also available as cryopreserved embryos from our Repository. Orders for cryopreserved embryos are supplied subject to a signed agreement that must be returned to the Customer Service Department after order placement. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos from our repository, please visit our Cryopreserved Embryos web page. Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.