Description

Strain Information

Type Mutant Strain; Transgenic; Additional information on Genetically Engineered Mutant Mice. Species laboratory mouse Generation N9   Donating Investigator Curt Sigmund,   University of Iowa

Appearance
black
Related Genotype: a/a

Description
Mice carrying the human renin transgene have high levels of expression in the kidney, adrenal gland, ovary, testis, lung and adipose. No expression is evident in the liver or brain. Active renin is released into the plasma of the transgenic mice. These mice are normotensive but a chronically hypertensive mouse can be created by breeding this strain with a strain carrying the human angiotensinogen transgene (C57BL/6-TgN(hAGT)2041Sig; 002749). The mean arterial pressure of the double transgenic is ~155 mmHg (controls ~111 mmHg). Both systolic and diastolic blood pressures are elevated.

Development
A human renin genomic clone that included all ten exons and intervening intron sequences was injected into B10C3F2 eggs. This transgene integrated into the X chromosome.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(hREN)9Sig/Y

        involves: C3H/HeRos * C57BL/10Ros

• cardiovascular system phenotype
• *normal* cardiovascular system phenotype (MGI Ref ID J:79859)
  • mice exhibit normal blood pressure

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

REN related

Cardiovascular Research
Hypertension (normotensive)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(hREN)9Sig
Allele Name		transgene insertion 9, Curt Sigmund
Allele Type		Transgenic (random, expressed)
Common Name(s)		R+;
Mutation Made By		Curt Sigmund,   University of Iowa
Strain of Origin		(C57BL/10Ros x C3H/HeRos)F2
Expressed Gene		REN, renin, human
Molecular Note		The transgene contained the entire human renin gene. Active renin is released into the plasma, and high levels of human renin expression are detected in kidney, adrenal gland, ovary, testis, lung and adipose tissue. Expression of human renin is not detected in liver or brain. [MGI Ref ID J:81877]

Genotyping

Genotyping Information

Genotyping Protocols

Tg(hREN)9Sig, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Sigmund CD; Jones CA; Kane CM; Wu C; Lang JA; Gross KW. 1992. Regulated tissue- and cell-specific expression of the human renin gene in transgenic mice. Circ Res 70(5):1070-9. [PubMed: 1568298]  [MGI Ref ID J:81877]

Additional References

Davisson RL; Yang G; Beltz TG; Cassell MD; Johnson AK; Sigmund CD. 1998. The brain renin-angiotensin system contributes to the hypertension in mice containing both the human renin and human angiotensinogen transgenes. Circ Res 83(10):1047-58. [PubMed: 9815152]  [MGI Ref ID J:79945]

Merrill DC; Thompson MW; Carney CL; Granwehr BP; Schlager G; Robillard JE; Sigmund CD. 1996. Chronic hypertension and altered baroreflex responses in transgenic mice containing the human renin and human angiotensinogen genes. J Clin Invest 97(4):1047-55. [PubMed: 8613528]  [MGI Ref ID J:79859]

Sinn PL; Davis DR; Sigmund CD. 1999. Highly regulated cell type-restricted expression of human renin in mice containing 140- or 160-kilobase pair P1 phage artificial chromosome transgenes. J Biol Chem 274(50):35785-93. [PubMed: 10585461]  [MGI Ref ID J:58897]

Tg(hREN)9Sig related

Baumbach GL; Sigmund CD; Faraci FM. 2003. Cerebral arteriolar structure in mice overexpressing human renin and angiotensinogen. Hypertension 41(1):50-5. [PubMed: 12511529]  [MGI Ref ID J:128819]

Davisson RL; Ding Y; Stec DE; Catterall JF; Sigmund CD. 1999. Novel mechanism of hypertension revealed by cell-specific targeting of human angiotensinogen in transgenic mice. Physiol Genomics 1(1):3-9. [PubMed: 11015555]  [MGI Ref ID J:135870]

Davisson RL; Kim HS; Krege JH; Lager DJ; Smithies O; Sigmund CD. 1997. Complementation of reduced survival, hypotension, and renal abnormalities in angiotensinogen-deficient mice by the human renin and human angiotensinogen genes. J Clin Invest 99(6):1258-64. [PubMed: 9077534]  [MGI Ref ID J:39934]

Davisson RL; Yang G; Beltz TG; Cassell MD; Johnson AK; Sigmund CD. 1998. The brain renin-angiotensin system contributes to the hypertension in mice containing both the human renin and human angiotensinogen transgenes. Circ Res 83(10):1047-58. [PubMed: 9815152]  [MGI Ref ID J:79945]

Didion SP; Ryan MJ; Baumbach GL; Sigmund CD; Faraci FM. 2002. Superoxide contributes to vascular dysfunction in mice that express human renin and angiotensinogen. Am J Physiol Heart Circ Physiol 283(4):H1569-76. [PubMed: 12234811]  [MGI Ref ID J:79459]

Didion SP; Sigmund CD; Faraci FM. 2000. Impaired endothelial function in transgenic mice expressing both human renin and human angiotensinogen. Stroke 31(3):760-4; discussion 765. [PubMed: 10700516]  [MGI Ref ID J:61307]

Ding Y; Stec DE; Sigmund CD. 2001. Genetic evidence that lethality in angiotensinogen-deficient mice is due to loss of systemic but not renal angiotensinogen. J Biol Chem 276(10):7431-6. [PubMed: 11096065]  [MGI Ref ID J:135051]

Iida S; Baumbach GL; Lavoie JL; Faraci FM; Sigmund CD; Heistad DD. 2005. Spontaneous stroke in a genetic model of hypertension in mice. Stroke 36(6):1253-8. [PubMed: 15914769]  [MGI Ref ID J:130409]

Merrill DC; Thompson MW; Carney CL; Granwehr BP; Schlager G; Robillard JE; Sigmund CD. 1996. Chronic hypertension and altered baroreflex responses in transgenic mice containing the human renin and human angiotensinogen genes. J Clin Invest 97(4):1047-55. [PubMed: 8613528]  [MGI Ref ID J:79859]

Sethi S; Iida S; Sigmund CD; Heistad DD. 2006. Renal thrombotic microangiopathy in a genetic model of hypertension in mice. Exp Biol Med (Maywood) 231(2):196-203. [PubMed: 16446496]  [MGI Ref ID J:129130]

Sherrod M; Davis DR; Zhou X; Cassell MD; Sigmund CD. 2005. Glial-specific ablation of angiotensinogen lowers arterial pressure in renin and angiotensinogen transgenic mice. Am J Physiol Regul Integr Comp Physiol 289(6):R1763-9. [PubMed: 16109805]  [MGI Ref ID J:130651]

Stec DE; Keen HL; Sigmund CD. 2002. Lower blood pressure in floxed angiotensinogen mice after adenoviral delivery of Cre-recombinase. Hypertension 39(2 Pt 2):629-33. [PubMed: 11882621]  [MGI Ref ID J:133558]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection.

Control Information

	Control
	Noncarrier
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.