Strain Name:

FVB/N-Tg(WapNotch4)10Rnc/J

Stock Number:

002755

Availability:

Repository-Cryopreserved

Use Restrictions Apply, see Terms of Use

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names FVB/N-TgN(WapInt3)10Rnc    (Changed: 15-DEC-04 )
Type Mutant Strain; Transgenic;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
GenerationN?+3F7pN1
 
Donating Investigator Robert Callahan,   National Institutes of Health

Appearance
albino
Related Genotype: Tyrc/Tyrc

Description
In transgenic mice carrying the WAP/Int3 construct, mammary ductal growth was unaffected in virgin females, but growth and differentiation of secretory lobules during gestation was profoundly inhibited. Mammary dysplasia and tumorigenesis occurred in all breeding females by 25 weeks of age. In non-breeding WAP/Int3 females mammary tumor incidence also reached 100% but only after 70 weeks. The WAP/Int3 mammary tumors were highly malignant and most tumor-bearing females, irrespective of breeding history developed metastatic lung lesions. FVB/WAP/Int3 females are unable to lactate. This phenotype is different from that of the FVB-TgN(MMTVInt3)3Rnc strain mice. In virgin and multiparous mice of that strain, mammary ductal growth and secretory lobule development were both curtailed. Mammary adenocarcinomas are observed as early as 7 weeks, and salivary adenocarcinomas were also observed. Males were sterile. Differences in the phenotype are attributed to the tissue-specific promoters used. The WAP promoter directs expression of the transgene to the secretory epithelium, whereas the MMTV LTR is more widely expressed and at an earlier stage of mammary ductal development.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Wap     (8 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms
      assigned by genotype

Tg(WapNotch4)10Rnc/Y

        involves: FVB/N
  • endocrine/exocrine gland phenotype
  • abnormal lactation (MGI Ref ID J:33193)
    • parous females fail to lactate
  • abnormal mammary gland growth during pregnancy (MGI Ref ID J:33193)
    • gestating females exhibit abnormal development of secretory lobules such that during late pregnancy, lobular development is severely retarded, and no secretory activity is apparent in the glands
    • pregnant and postpartum mammary glands show dysplastic ducts and poorly formed alveolar buds
    • acini in day 1 postpartum lactating glands have small lumina, constricted by numerous nonpolar, undifferentiated epithelial cells, which contain little or no endoplasmic reticulum, secretory vesicles, or lipid droplets and no secretory particles are present in acinar lumina
    • in ducts of postpartum females, the luminal epithelial cells appear condensed and moribund
    • however, mammary ductal growth is unaffected in virgin females
  • reproductive system phenotype
  • abnormal lactation (MGI Ref ID J:33193)
    • parous females fail to lactate
  • abnormal mammary gland growth during pregnancy (MGI Ref ID J:33193)
    • gestating females exhibit abnormal development of secretory lobules such that during late pregnancy, lobular development is severely retarded, and no secretory activity is apparent in the glands
    • pregnant and postpartum mammary glands show dysplastic ducts and poorly formed alveolar buds
    • acini in day 1 postpartum lactating glands have small lumina, constricted by numerous nonpolar, undifferentiated epithelial cells, which contain little or no endoplasmic reticulum, secretory vesicles, or lipid droplets and no secretory particles are present in acinar lumina
    • in ducts of postpartum females, the luminal epithelial cells appear condensed and moribund
    • however, mammary ductal growth is unaffected in virgin females
  • tumorigenesis
  • lung tumor (MGI Ref ID J:33193)
    • most tumor-bearing females, irrespective of breeding history, develop metastatic lung tumors
  • mammary adenocarcinoma (MGI Ref ID J:33193)
    • all virgin and multiparous females develop mammary tumors, with a 50% incidence of tumors in virgins at 11 months of age and in breeding females at 5 months

Tg(WapNotch4)10Rnc/Y

        involves: FVB/N
  • tumorigenesis
  • *normal* tumorigenesis (MGI Ref ID J:33193)
    • males do not form mammary adenocarcinomas as are seen in females

Genes & Alleles

Gene & Allele Information

 
Allele Symbol Tg(WapNotch4)10Rnc
Allele Name transgene insertion 10, Robert Callahan
Allele Type Transgenic (random, expressed)
Common Name(s) Tg(WapInt3)10Rnc; WAP/Int3; Wap-int3 tg;
Strain of OriginFVB/N
Expressed Gene Notch4, Notch homolog 4 (Drosophila), rat
Promoter Wap, whey acidic protein, mouse, laboratory
Molecular Note The transgene insert consisted of the mouse whey acidic acid promoter and the portion of the Notch4 gene encoding the intracellular domain. Wap promoter activity is restricted to the secretory mammary epithelial population. Four founder mice were identified. One line established from founder 10. Transmission analysis indicates that the transgene inserted into the X chromosome. [MGI Ref ID J:33193]
 
 

Genotyping

Genotyping Information

Genotyping Protocols

Tg(WapNotch4)10Rnc, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Gallahan D; Jhappan C; Robinson G; Hennighausen L; Sharp R; Kordon E; Callahan R; Merlino G; Smith GH. 1996. Expression of a truncated Int3 gene in developing secretory mammary epithelium specifically retards lobular differentiation resulting in tumorigenesis. Cancer Res 56(8):1775-85. [PubMed: 8620493]  [MGI Ref ID J:33193]

Additional References

Tg(WapNotch4)10Rnc related

Cardiff RD; Anver MR; Gusterson BA; Hennighausen L; Jensen RA; Merino MJ; Rehm S; Russo J; Tavassoli FA; Wakefield LM; Ward JM; Green JE. 2000. The mammary pathology of genetically engineered mice: the consensus report and recommendations from the Annapolis meeting [see comments] Oncogene 19(8):968-88. [PubMed: 10713680]  [MGI Ref ID J:61035]

Raafat A; Zoltan-Jones A; Strizzi L; Bargo S; Kimura K; Salomon D; Callahan R. 2007. Kit and PDGFR-alpha activities are necessary for Notch4/Int3-induced tumorigenesis. Oncogene 26(5):662-72. [PubMed: 16878155]  [MGI Ref ID J:117838]

Robinson GW; Wagner KU; Hennighausen L. 2001. Functional mammary gland development and oncogene-induced tumor formation are not affected by the absence of the retinoblastoma gene. Oncogene 20(48):7115-9. [PubMed: 11704837]  [MGI Ref ID J:73006]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations View International pricing
Price (US dollars $)
Cryorecovery Fee $1900.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Pricing for International shipping destinations View USA Canada and Mexico pricing
Price (US dollars $)
Cryorecovery Fee $2470.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Additional Supply Details

Supply Details

Standard SupplyCryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.
Supply Notes
  • Cryorecovery - Standard.
    At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
    One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

  • This strain is included in the Induced Mutant Resource Colony collection.
  • Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

  Control
   Noncarrier
   001800 FVB/NJ
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions


See Terms of Use


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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Contact Information
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Fax: 207.288.6150
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Terms of Use

Terms of Use


General Terms and Conditions


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Contact information

General inquiries

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fax:207-288-6655

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