Strain Name:

B6.129S2-Cd40lgtm1Imx/J

Stock Number:

002770

Availability:

Repository- Live

Description

Strain Information

Former Names B6.129S2-Cd40tm1Imx/J    (Changed: 12-OCT-05 )
B6.129S2-Tnfsf5tm1Imx/J    (Changed: 30-SEP-05 )
Cd40l    (Changed: 15-DEC-04 )
Type Congenic; Mutant Strain; Targeted Mutation;
Mating SystemHomozygote x Hemizygote         (Female x Male)
Specieslaboratory mouse
Background Strain C57BL/6J
Donor Strain 129S2 via D3 ES cell line
GenerationN?+5F27 (20-DEC-06)
 
Donating Investigator IMR Colony,   The Jackson Laboratory

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. Percentages of B and T cell subpopulations is normal. Homozygotes do show selective deficiencies in humoral immunity (low basal serum isotype levels and undetectable IgE) as well as abnormal secondary antigen-specific responses to immunization with a thymus-dependent antigen. The phenotype of the mice resembles human X-linked hyper IgM syndrome.

Development
A targeting vector containing a PGKneo cassette was used to exons 3 and 4. The construct was electroporated into 129S2/SvPas derived D3 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting male chimeric animals were crossed to C57BL/6 female mice. Heterozygous (129/SV X C57BL/6)F1 females were crossed to C57BL/6 males to generate hemizygous males. The mice were subsequently backcrossed on to the C57BL/6J background for 5 generations.

Control Information

  Allele   Control
 Cd40lgtm1Imx  000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Cd40lgtm1Imx allele
002428   B6;129S2-Cd40lgtm1Imx/J
View Strains carrying   Cd40lgtm1Imx     (1 strain)

Additional Web Information

Congenic Nomenclature
Genetic Quality Control Annual Report

Phenotype

Phenotype Information

Related Disease (OMIM) Terms

NOTImmunodeficiency with Hyper-IgM, Type 1; HIGM1

Mammalian Phenotype Terms assigned by genotype

Cd40lgtm1Imx/Cd40lgtm1Imx

        B6.129S2-Cd40lgtm1Imx/J
  • nervous system phenotype
  • abnormal thalamus morphology (MGI Ref ID J:106362)
    • loss of neurons in the submedial thalamic nucleus due to thiamine deficiency slower than in controls
    • after 11 days of thiamine deficiency, neuron loss in the submedial thalamic nucleus due to thiamine deficiency is about 90%

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Cd40lgtm1Imx/Cd40lgtm1Imx

        involves: 129S2/SvPas * C57BL/6J * BALB/c
  • immune system phenotype
  • abnormal T cell clonal deletion (MGI Ref ID J:110925)
    • mice show complete rescue of CD8+ and CD4+ V beta11+ and beta6+ bearing CD4+ and CD8+ thymocytes from deletion in the thymus and periphery and Vbeta5+ CD4+ cells, but not Vbeta5+ bearing CD8+ thymocytes
  • hematopoietic system phenotype
  • abnormal T cell clonal deletion (MGI Ref ID J:110925)
    • mice show complete rescue of CD8+ and CD4+ V beta11+ and beta6+ bearing CD4+ and CD8+ thymocytes from deletion in the thymus and periphery and Vbeta5+ CD4+ cells, but not Vbeta5+ bearing CD8+ thymocytes

Cd40lgtm1Imx/Cd40lgtm1Imx

        involves: 129S2/SvPas * C57BL/6
  • reproductive system phenotype
  • *normal* reproductive system phenotype (MGI Ref ID J:21137)
    • homozygous females are viable and fertile
  • homeostasis/metabolism phenotype
  • abnormal blood coagulation (MGI Ref ID J:75152)
    • thrombi formed in response to FeCl3 injury are fragile and prone to rupture and to embolizing
    • smaller thrombi with lower platelet density
    • clotting times are normal
  • immune system phenotype
  • abnormal microglial cell physiology (MGI Ref ID J:115487)
    • increased microgliosis
  • increased susceptibility to prion infection (MGI Ref ID J:115487)
    • early onset of disease when infected with scrapie
    • succumb to infection after 144 days as opposed to 184 days for controls
    • symptoms of scrapie infection equivalent to 1000X higher dose
  • nervous system phenotype
  • abnormal nervous system physiology (MGI Ref ID J:115487)
    • abnormal microglial cell physiology (MGI Ref ID J:115487)
      • increased microgliosis
    • neurodegeneration (MGI Ref ID J:115487)
      • increased apoptosis
      • spongiform encephalopathy (MGI Ref ID J:115487)
        • vacuolation resulting from scrapie infection more pronounced than in controls
  • loss of cortex neurons (MGI Ref ID J:115487)
    • more severe loss of parvalbumin positive neurons in the cortex as a result of scrapie infection than occurs in controls

Cd40lgtm1Imx/Y

        involves: 129S2/SvPas * C57BL/6
  • immune system phenotype
  • *normal* immune system phenotype (MGI Ref ID J:21137)
    • serum IgM and IgG3 levels are more or less normal at all ages
    • abnormal immune system organ morphology (MGI Ref ID J:21137)
      • abnormal lymph node morphology (MGI Ref ID J:21137)
        • abnormal lymph node cellularity (MGI Ref ID J:21137)
          • about 1/3 that of controls while overall T and B cell numbers are normal
        • absent lymph node germinal center (MGI Ref ID J:21137)
          • failure to form germinal centers in inguinal lymph nodes although primary follicle formation is normal
      • absent spleen germinal center (MGI Ref ID J:21137)
        • failure to form germinal centers
    • abnormal immune system physiology (MGI Ref ID J:21137)
      • abnormal antigen presentation (MGI Ref ID J:21137)
        • unable to mount a secondary response to TNP-KLH antigen (T cell dependent)
        • T cell independent responses are normal as is isotype switching for these antigens
      • abnormal class switch recombination (MGI Ref ID J:21137)
      • decreased immunoglobulin level (MGI Ref ID J:21137)
        • decreased IgA level (MGI Ref ID J:21137)
        • decreased IgE level (MGI Ref ID J:21137)
          • undetectable
        • decreased IgG1 level (MGI Ref ID J:21137)
        • decreased IgG2b level (MGI Ref ID J:21137)
  • hematopoietic system phenotype
  • abnormal class switch recombination (MGI Ref ID J:21137)
  • absent spleen germinal center (MGI Ref ID J:21137)
    • failure to form germinal centers

Research Applications

This mouse can be used to support research in many areas including:

Cd40lgtm1Imx related

Cancer Research
Genes Regulating Growth and Proliferation

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers

Mouse/Human Gene Homologs
immunodeficiency with hyper-IgM, type I (hyper-IgM syndrome)

Genes & Alleles

Gene & Allele Information

Allele Symbol Cd40lgtm1Imx
Allele Name targeted mutation 1, Immunex
Common Name(s) CD154-; CD40L-; CD40LKO; Tnfsf5tm1Imx;
Mutation Made By Jacques Peschon,   Amgen
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Cd40lg, CD40 ligand
Chromosome X
Gene Common Name(s) CD154; CD40 antigen ligand; CD40L; Cd40l; HIGM1; IGM; IMD3; Ly-62; Ly62; T-BAM; TNFSF5; TRAP; Tnfsf5; gp39; hCD40L; lymphocyte antigen 62; tumor necrosis factor (ligand) superfamily, member 5;
Molecular Note Exons 3 and 4 were deleted by the insertion of a neomycin cassette. Homozygous mutant animals fail to express functional protein on the cell surface. [MGI Ref ID J:21137]

Genotyping

Genotyping Information

Genotyping Protocols

Cd40lgtm1Imx, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Renshaw BR; Fanslow WC 3rd; Armitage RJ; Campbell KA; Liggitt D; Wright B; Davison BL; Maliszewski CR. 1994. Humoral immune responses in CD40 ligand-deficient mice. J Exp Med 180(5):1889-900. [PubMed: 7964465]  [MGI Ref ID J:21137]

Additional References

Lazarevic V; Myers AJ; Scanga CA; Flynn JL. 2003. CD40, but not CD40L, is required for the optimal priming of T cells and control of aerosol M. tuberculosis infection. Immunity 19(6):823-35. [PubMed: 14670300]  [MGI Ref ID J:86998]

MacDonald AS; Patton EA; La Flamme AC; Araujo MI; Huxtable CR; Bauman B; Pearce EJ. 2002. Impaired Th2 Development and Increased Mortality During Schistosoma mansoni Infection in the Absence of CD40/CD154 Interaction. J Immunol 168(9):4643-9. [PubMed: 11971013]  [MGI Ref ID J:76160]

Murray HW; Lu CM; Brooks EB; Fichtl RE; DeVecchio JL; Heinzel FP. 2003. Modulation of T-cell costimulation as immunotherapy or immunochemotherapy in experimental visceral leishmaniasis. Infect Immun 71(11):6453-62. [PubMed: 14573667]  [MGI Ref ID J:86275]

Piguet PF; Kan CD; Vesin C; Rochat A; Donati Y; Barazzone C. 2001. Role of CD40-CVD40L in mouse severe malaria. Am J Pathol 159(2):733-42. [PubMed: 11485931]  [MGI Ref ID J:70869]

Sun JC; Bevan MJ. 2004. Cutting edge: long-lived CD8 memory and protective immunity in the absence of CD40 expression on CD8 T cells. J Immunol 172(6):3385-9. [PubMed: 15004136]  [MGI Ref ID J:88609]

Cd40lgtm1Imx related

Amrani A; Serra P; Yamanouchi J; Han B; Thiessen S; Verdaguer J; Santamaria P. 2002. CD154-dependent priming of diabetogenic CD4(+) T cells dissociated from activation of antigen-presenting cells. Immunity 16(5):719-32. [PubMed: 12049723]  [MGI Ref ID J:76802]

Andersen C; Jensen T; Nansen A; Marker O; Thomsen AR. 1999. CD4(+) T cell-mediated protection against a lethal outcome of systemic infection with vesicular stomatitis virus requires CD40 ligand expression, but not IFN-gamma or IL-4. Int Immunol 11(12):2035-42. [PubMed: 10590269]  [MGI Ref ID J:110491]

Andre P; Prasad KS; Denis CV; He M; Papalia JM; Hynes RO; Phillips DR; Wagner DD. 2002. CD40L stabilizes arterial thrombi by a beta3 integrin--dependent mechanism. Nat Med 8(3):247-52. [PubMed: 11875495]  [MGI Ref ID J:75152]

Andreasen SO; Christensen JE; Marker O; Thomsen AR. 2000. Role of CD40 ligand and CD28 in induction and maintenance of antiviral CD8+ effector T cell responses. J Immunol 164(7):3689-97. [PubMed: 10725727]  [MGI Ref ID J:123023]

Barazzone Argiroffo C; Donati YR; Boccard J; Rochat AF; Vesin C; Kan CD; Piguet PF. 2002. CD40-CD40 ligand disruption does not prevent hyperoxia-induced injury. Am J Pathol 160(1):67-71. [PubMed: 11786400]  [MGI Ref ID J:108197]

Belkaid Y; Mendez S; Lira R; Kadambi N; Milon G; Sacks D. 2000. A natural model of Leishmania major infection reveals a prolonged 'silent' phase of parasite amplification in the skin before the onset of lesion formation and immunity. J Immunol 165(2):969-77. [PubMed: 10878373]  [MGI Ref ID J:120233]

Bry L; Brigl M; Brenner MB. 2006. CD4+-T-cell effector functions and costimulatory requirements essential for surviving mucosal infection with Citrobacter rodentium. Infect Immun 74(1):673-81. [PubMed: 16369024]  [MGI Ref ID J:104251]

Burwinkel M; Schwarz A; Riemer C; Schultz J; van Landeghem F; Baier M. 2004. Rapid disease development in scrapie-infected mice deficient for CD40 ligand. EMBO Rep 5(5):527-31. [PubMed: 15071493]  [MGI Ref ID J:115487]

Campos-Neto A; Ovendale P; Bement T; Koppi TA; Fanslow WC; Rossi MA; Alderson MR. 1998. CD40 ligand is not essential for the development of cell-mediated immunity and resistance to Mycobacterium tuberculosis. J Immunol 160(5):2037-41. [PubMed: 9498737]  [MGI Ref ID J:123032]

Chan K; Lee DJ; Schubert A; Tang CM; Crain B; Schoenberger SP; Corr M. 2001. The roles of MHC class II, CD40, and B7 costimulation in CTL induction by plasmid DNA. J Immunol 166(5):3061-6. [PubMed: 11207256]  [MGI Ref ID J:126695]

Chougnet C; Freitag C; Schito M; Thomas EK; Sher A; Shearer GM. 2001. In vivo CD40-CD154 (CD40 ligand) interaction induces integrated HIV expression by APC in an HIV-1-transgenic mouse model. J Immunol 166(5):3210-7. [PubMed: 11207274]  [MGI Ref ID J:126480]

Christensen JE; Christensen JP; Kristensen NN; Hansen NJ; Stryhn A; Thomsen AR. 2002. Role of CD28 co-stimulation in generation and maintenance of virus-specific T cells. Int Immunol 14(7):701-11. [PubMed: 12096029]  [MGI Ref ID J:113535]

Crow AR; Leytin V; Starkey AF; Rand ML; Lazarus AH. 2003. CD154 (CD40 ligand)-deficient mice exhibit prolonged bleeding time and decreased shear-induced platelet aggregates. J Thromb Haemost 1(4):850-2. [PubMed: 12871426]  [MGI Ref ID J:103954]

Demirci G; Amanullah F; Kewalaramani R; Yagita H; Strom TB; Sayegh MH; Li XC. 2004. Critical role of OX40 in CD28 and CD154-independent rejection. J Immunol 172(3):1691-8. [PubMed: 14734751]  [MGI Ref ID J:87657]

Dole VS; Bergmeier W; Mitchell HA; Eichenberger SC; Wagner DD. 2005. Activated platelets induce Weibel-Palade-body secretion and leukocyte rolling in vivo: role of P-selectin. Blood 106(7):2334-9. [PubMed: 15956287]  [MGI Ref ID J:119376]

Elzey BD; Grant JF; Sinn HW; Nieswandt B; Waldschmidt TJ; Ratliff TL. 2005. Cooperation between platelet-derived CD154 and CD4+ T cells for enhanced germinal center formation. J Leukoc Biol 78(1):80-4. [PubMed: 15899982]  [MGI Ref ID J:99287]

Elzey BD; Tian J; Jensen RJ; Swanson AK; Lees JR; Lentz SR; Stein CS; Nieswandt B; Wang Y; Davidson BL; Ratliff TL. 2003. Platelet-mediated modulation of adaptive immunity. A communication link between innate and adaptive immune compartments. Immunity 19(1):9-19. [PubMed: 12871635]  [MGI Ref ID J:84573]

Erickson LD; Foy TM; Waldschmidt TJ. 2001. Murine B1 B cells require IL-5 for optimal T cell-dependent activation. J Immunol 166(3):1531-9. [PubMed: 11160193]  [MGI Ref ID J:127070]

Fang M; Sigal LJ. 2005. Antibodies and CD8+ T cells are complementary and essential for natural resistance to a highly lethal cytopathic virus. J Immunol 175(10):6829-36. [PubMed: 16272340]  [MGI Ref ID J:119697]

Fischbein MP; Ardehali A; Yun J; Schoenberger S; Laks H; Irie Y; Dempsey P; Cheng G; Fishbein MC; Bonavida B. 2000. CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants. J Immunol 165(12):7316-22. [PubMed: 11120867]  [MGI Ref ID J:118395]

Gaspal FM; McConnell FM; Kim MY; Gray D; Kosco-Vilbois MH; Raykundalia CR; Botto M; Lane PJ. 2006. The generation of thymus-independent germinal centers depends on CD40 but not on CD154, the T cell-derived CD40-ligand. Eur J Immunol 36(7):1665-73. [PubMed: 16783845]  [MGI Ref ID J:115798]

Golovkina TV; Shlomchik M; Hannum L; Chervonsky A. 1999. Organogenic role of B lymphocytes in mucosal immunity. Science 286(5446):1965-8. [PubMed: 10583962]  [MGI Ref ID J:124532]

Gorbachev AV; Heeger PS; Fairchild RL. 2001. CD4+ and CD8+ T cell priming for contact hypersensitivity occurs independently of CD40-CD154 interactions. J Immunol 166(4):2323-32. [PubMed: 11160289]  [MGI Ref ID J:126997]

Hao S; Yuan J; Xiang J. 2007. Nonspecific CD4+ T cells with uptake of antigen-specific dendritic cell-released exosomes stimulate antigen-specific CD8+ CTL responses and long-term T cell memory. J Leukoc Biol 82(4):829-38. [PubMed: 17626150]  [MGI Ref ID J:125204]

Hermans IF; Silk JD; Gileadi U; Salio M; Mathew B; Ritter G; Schmidt R; Harris AL; Old L; Cerundolo V. 2003. NKT cells enhance CD4+ and CD8+ T cell responses to soluble antigen in vivo through direct interaction with dendritic cells. J Immunol 171(10):5140-7. [PubMed: 14607913]  [MGI Ref ID J:119212]

Hernandez-Novoa B; Bishop L; Logun C; Munson PJ; Elnekave E; Rangel ZG; Barb J; Danner RL; Kovacs JA. 2008. Immune responses to Pneumocystis murina are robust in healthy mice but largely absent in CD40 ligand-deficient mice. J Leukoc Biol 84(2):420-30. [PubMed: 18467653]  [MGI Ref ID J:138448]

Hu HM; Winter H; Ma J; Croft M; Urba WJ; Fox BA. 2002. CD28, TNF receptor, and IL-12 are critical for CD4-independent cross-priming of therapeutic antitumor CD8+ T cells. J Immunol 169(9):4897-904. [PubMed: 12391201]  [MGI Ref ID J:118780]

Iannacone M; Sitia G; Isogawa M; Whitmire JK; Marchese P; Chisari FV; Ruggeri ZM; Guidotti LG. 2008. Platelets prevent IFN-alpha/beta-induced lethal hemorrhage promoting CTL-dependent clearance of lymphocytic choriomeningitis virus. Proc Natl Acad Sci U S A 105(2):629-34. [PubMed: 18184798]  [MGI Ref ID J:131089]

Ishikawa M; Vowinkel T; Stokes KY; Arumugam TV; Yilmaz G; Nanda A; Granger DN. 2005. CD40/CD40 ligand signaling in mouse cerebral microvasculature after focal ischemia/reperfusion. Circulation 111(13):1690-6. [PubMed: 15795333]  [MGI Ref ID J:108988]

Jenkins SJ; Perona-Wright G; MacDonald AS. 2008. Full development of Th2 immunity requires both innate and adaptive sources of CD154. J Immunol 180(12):8083-92. [PubMed: 18523272]  [MGI Ref ID J:137237]

Jeurissen A; Billiau AD; Moens L; Shengqiao L; Landuyt W; Wuyts G; Boon L; Waer M; Ceuppens JL; Bossuyt X. 2006. CD4+ T lymphocytes expressing CD40 ligand help the IgM antibody response to soluble pneumococcal polysaccharides via an intermediate cell type. J Immunol 176(1):529-36. [PubMed: 16365447]  [MGI Ref ID J:126613]

Kamperschroer C; Roberts DM; Zhang Y; Weng NP; Swain SL. 2008. SAP enables T cells to help B cells by a mechanism distinct from Th cell programming or CD40 ligand regulation. J Immunol 181(6):3994-4003. [PubMed: 18768854]  [MGI Ref ID J:139100]

Ke ZJ; Calingasan NY; DeGiorgio LA; Volpe BT; Gibson GE. 2005. CD40-CD40L interactions promote neuronal death in a model of neurodegeneration due to mild impairment of oxidative metabolism. Neurochem Int 47(3):204-15. [PubMed: 15885854]  [MGI Ref ID J:106362]

Ke ZJ; Calingasan NY; Karuppagounder SS; DeGiorgio LA; Volpe BT; Gibson GE. 2005. CD40L deletion delays neuronal death in a model of neurodegeneration due to mild impairment of oxidative metabolism. J Neuroimmunol 164(1-2):85-92. [PubMed: 15904977]  [MGI Ref ID J:106388]

Kemball CC; Lee ED; Szomolanyi-Tsuda E; Pearson TC; Larsen CP; Lukacher AE. 2006. Costimulation requirements for antiviral CD8+ T cells differ for acute and persistent phases of polyoma virus infection. J Immunol 176(3):1814-24. [PubMed: 16424212]  [MGI Ref ID J:126419]

Khan WI; Motomura Y; Blennerhassett PA; Kanbayashi H; Varghese AK; El-Sharkawy RT; Gauldie J; Collins SM. 2005. Disruption of CD40-CD40 ligand pathway inhibits the development of intestinal muscle hypercontractility and protective immunity in nematode infection. Am J Physiol Gastrointest Liver Physiol 288(1):G15-22. [PubMed: 15308470]  [MGI Ref ID J:96181]

Kweon MN; Fujihashi K; Wakatsuki Y; Koga T; Yamamoto M; McGhee JR; Kiyono H. 1999. Mucosally induced systemic T cell unresponsiveness to ovalbumin requires CD40 ligand-CD40 interactions. J Immunol 162(4):1904-9. [PubMed: 9973457]  [MGI Ref ID J:112042]

Lazarevic V; Myers AJ; Scanga CA; Flynn JL. 2003. CD40, but not CD40L, is required for the optimal priming of T cells and control of aerosol M. tuberculosis infection. Immunity 19(6):823-35. [PubMed: 14670300]  [MGI Ref ID J:86998]

Leadbetter EA; Brigl M; Illarionov P; Cohen N; Luteran MC; Pillai S; Besra GS; Brenner MB. 2008. NK T cells provide lipid antigen-specific cognate help for B cells. Proc Natl Acad Sci U S A 105(24):8339-44. [PubMed: 18550809]  [MGI Ref ID J:137193]

Lee BO; Moyron-Quiroz J; Rangel-Moreno J; Kusser KL; Hartson L; Sprague F; Lund FE; Randall TD. 2003. CD40, but not CD154, expression on B cells is necessary for optimal primary B cell responses. J Immunol 171(11):5707-17. [PubMed: 14634078]  [MGI Ref ID J:119308]

Lefrancois L; Olson S; Masopust D. 1999. A critical role for CD40-CD40 ligand interactions in amplification of the mucosal CD8 T cell response. J Exp Med 190(9):1275-84. [PubMed: 10544199]  [MGI Ref ID J:58316]

Lei XF; Ohkawara Y; Stampfli MR; Mastruzzo C; Marr RA; Snider D; Xing Z; Jordana M. 1998. Disruption of antigen-induced inflammatory responses in CD40 ligand knockout mice. J Clin Invest 101(6):1342-53. [PubMed: 9502776]  [MGI Ref ID J:46546]

Lentz VM; Manser T. 2000. Self-limiting systemic autoimmune disease during reconstitution of T cell-deficient mice with syngeneic T cells: support for a multifaceted role of T cells in the maintenance of peripheral B cell tolerance. Int Immunol 12(11):1483-97. [PubMed: 11058568]  [MGI Ref ID J:110494]

Li R; Page DM. 2001. Requirement for a complex array of costimulators in the negative selection of autoreactive thymocytes in vivo. J Immunol 166(10):6050-6. [PubMed: 11342622]  [MGI Ref ID J:110925]

Li W; Buzoni-Gatel D; Debbabi H; Hu MS; Mennechet FJ; Durell BG; Noelle RJ; Kasper LH. 2002. CD40/CD154 ligation is required for the development of acute ileitis following oral infection with an intracellular pathogen in mice. Gastroenterology 122(3):762-73. [PubMed: 11875009]  [MGI Ref ID J:75019]

Linden AM; Baez M; Bergeron M; Schoepp DD. 2003. Increased c-Fos expression in the centromedial nucleus of the thalamus in metabotropic glutamate 8 receptor knockout mice following the elevated plus maze test. Neuroscience 121(1):167-78. [PubMed: 12946709]  [MGI Ref ID J:109308]

Lode HN; Xiang R; Pertl U; Forster E; Schoenberger SP; Gillies SD; Reisfeld RA. 2000. Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction. J Clin Invest 105(11):1623-30. [PubMed: 10841521]  [MGI Ref ID J:62762]

Lund FE; Schuer K; Hollifield M; Randall TD; Garvy BA. 2003. Clearance of Pneumocystis carinii in mice is dependent on B cells but not on P carinii-specific antibody. J Immunol 171(3):1423-30. [PubMed: 12874234]  [MGI Ref ID J:120666]

MacDonald AS; Patton EA; La Flamme AC; Araujo MI; Huxtable CR; Bauman B; Pearce EJ. 2002. Impaired Th2 Development and Increased Mortality During Schistosoma mansoni Infection in the Absence of CD40/CD154 Interaction. J Immunol 168(9):4643-9. [PubMed: 11971013]  [MGI Ref ID J:76160]

Mackey MF; Gunn JR; Maliszewsky C; Kikutani H; Noelle RJ; Barth RJ Jr. 1998. Dendritic cells require maturation via CD40 to generate protective antitumor immunity. J Immunol 161(5):2094-8. [PubMed: 9725199]  [MGI Ref ID J:118387]

Martin-Fontecha A; Assarsson E; Carbone E; Karre K; Ljunggren HG. 1999. Triggering of murine NK cells by CD40 and CD86 (B7-2). J Immunol 162(10):5910-6. [PubMed: 10229827]  [MGI Ref ID J:110890]

McCoy KD; Harris NL; Diener P; Hatak S; Odermatt B; Hangartner L; Senn BM; Marsland BJ; Geuking MB; Hengartner H; Macpherson AJ; Zinkernagel RM. 2006. Natural IgE production in the absence of MHC Class II cognate help. Immunity 24(3):329-39. [PubMed: 16546101]  [MGI Ref ID J:113324]

Moodycliffe AM; Shreedhar V; Ullrich SE; Walterscheid J; Bucana C; Kripke ML; Flores-Romo L. 2000. CD40-CD40 ligand interactions in vivo regulate migration of antigen-bearing dendritic cells from the skin to draining lymph nodes. J Exp Med 191(11):2011-20. [PubMed: 10839815]  [MGI Ref ID J:62702]

Nashleanas M; Scott P. 2000. Activated T cells induce macrophages to produce NO and control Leishmania major in the absence of tumor necrosis factor receptor p55. Infect Immun 68(3):1428-34. [PubMed: 10678956]  [MGI Ref ID J:60570]

Netea MG; Meer JW; Verschueren I; Kullberg BJ. 2002. CD40/CD40 ligand interactions in the host defense against disseminated Candida albicans infection: the role of macrophage-derived nitric oxide. Eur J Immunol 32(5):1455-63. [PubMed: 11981834]  [MGI Ref ID J:115566]

Padigel UM; Perrin PJ; Farrell JP. 2001. The Development of a Th1-Type Response and Resistance to Leishmania major Infection in the Absence of CD40-CD40L Costimulation. J Immunol 167(10):5874-9. [PubMed: 11698463]  [MGI Ref ID J:72682]

Piguet PF; Kan CD; Vesin C; Rochat A; Donati Y; Barazzone C. 2001. Role of CD40-CVD40L in mouse severe malaria. Am J Pathol 159(2):733-42. [PubMed: 11485931]  [MGI Ref ID J:70869]

Pochanke V; Hatak S; Hengartner H; Zinkernagel RM; McCoy KD. 2006. Induction of IgE and allergic-type responses in fur mite-infested mice. Eur J Immunol 36(9):2434-45. [PubMed: 16909433]  [MGI Ref ID J:116736]

Reichmann G; Walker W; Villegas EN; Craig L; Cai G; Alexander J; Hunter CA. 2000. The CD40/CD40 ligand interaction is required for resistance to toxoplasmic encephalitis. Infect Immun 68(3):1312-8. [PubMed: 10678943]  [MGI Ref ID J:60577]

Remskar M; Li H; Chyu KY; Shah PK; Cercek B. 2001. Absence of CD40 signaling is associated with an increase in intimal thickening after arterial injury. Circ Res 88(4):390-4. [PubMed: 11230105]  [MGI Ref ID J:115587]

Roy V; Bonventi G; Cai Y; Macleod R; Wither JE. 2007. Immune mechanisms leading to abnormal B cell selection and activation in New Zealand Black mice. Eur J Immunol 37(9):2645-56. [PubMed: 17668901]  [MGI Ref ID J:124351]

Scott MJ; Hoth JJ; Stagner MK; Gardner SA; Peyton JC; Cheadle WG. 2004. CD40-CD154 interactions between macrophages and natural killer cells during sepsis are critical for macrophage activation and are not interferon gamma dependent. Clin Exp Immunol 137(3):469-77. [PubMed: 15320895]  [MGI Ref ID J:92312]

Serra P; Amrani A; Yamanouchi J; Han B; Thiessen S; Utsugi T; Verdaguer J; Santamaria P. 2003. CD40 ligation releases immature dendritic cells from the control of regulatory CD4+CD25+ T cells. Immunity 19(6):877-89. [PubMed: 14670304]  [MGI Ref ID J:86995]

Shepherd DM; Kerkvliet NI. 1999. Disruption of CD154:CD40 blocks generation of allograft immunity without affecting APC activation. J Immunol 163(5):2470-7. [PubMed: 10452982]  [MGI Ref ID J:57099]

Shimizu K; Schonbeck U; Mach F; Libby P; Mitchell RN. 2000. Host CD40 ligand deficiency induces long-term allograft survival and donor-specific tolerance in mouse cardiac transplantation but does not prevent graft arteriosclerosis. J Immunol 165(6):3506-18. [PubMed: 10975872]  [MGI Ref ID J:64490]

Sprague DL; Elzey BD; Crist SA; Waldschmidt TJ; Jensen RJ; Ratliff TL. 2008. Platelet-mediated modulation of adaptive immunity: unique delivery of CD154 signal by platelet-derived membrane vesicles. Blood 111(10):5028-36. [PubMed: 18198347]  [MGI Ref ID J:135339]

Sugaya M; Nakamura K; Asahina A; Fujita H; Tada Y; Torii H; Tamaki K. 2005. Signaling through CD40 ligand decreases CD80 expression on murine Langerhans cells and enhances IL-12 p40 production. Biochem Biophys Res Commun 331(4):1045-52. [PubMed: 15882983]  [MGI Ref ID J:98321]

Sun JC; Bevan MJ. 2004. Cutting edge: long-lived CD8 memory and protective immunity in the absence of CD40 expression on CD8 T cells. J Immunol 172(6):3385-9. [PubMed: 15004136]  [MGI Ref ID J:88609]

Tahara M; Pergolizzi RG; Kobayashi H; Krause A; Luettich K; Lesser ML; Crystal RG. 2004. Trans-splicing repair of CD40 ligand deficiency results in naturally regulated correction of a mouse model of hyper-IgM X-linked immunodeficiency. Nat Med 10(8):835-41. [PubMed: 15273748]  [MGI Ref ID J:91607]

Tan JT; Whitmire JK; Ahmed R; Pearson TC; Larsen CP. 1999. 4-1BB ligand, a member of the TNF family, is important for the generation of antiviral CD8 T cell responses. J Immunol 163(9):4859-68. [PubMed: 10528187]  [MGI Ref ID J:118557]

TeKippe M; Harrison DE; Chen J. 2003. Expansion of hematopoietic stem cell phenotype and activity in Trp53-null mice. Exp Hematol 31(6):521-7. [PubMed: 12829028]  [MGI Ref ID J:115677]

Thomsen AR; Nansen A; Christensen JP; Andreasen SO; Marker O. 1998. CD40 ligand is pivotal to efficient control of virus replication in mice infected with lymphocytic choriomeningitis virus. J Immunol 161(9):4583-90. [PubMed: 9794385]  [MGI Ref ID J:115236]

VanLith ML; Kohlgraf KG; Sivinski CL; Tempero RM; Hollingsworth MA. 2002. MUC1-specific anti-tumor responses: molecular requirements for CD4-mediated responses. Int Immunol 14(8):873-82. [PubMed: 12147624]  [MGI Ref ID J:113544]

Villegas EN; Elloso MM; Reichmann G; Peach R; Hunter CA. 1999. Role of CD28 in the generation of effector and memory responses required for resistance to Toxoplasma gondii. J Immunol 163(6):3344-53. [PubMed: 10477604]  [MGI Ref ID J:119602]

Vowinkel T; Anthoni C; Wood KC; Stokes KY; Russell J; Gray L; Bharwani S; Senninger N; Alexander JS; Krieglstein CF; Grisham MB; Granger DN. 2007. CD40-CD40 ligand mediates the recruitment of leukocytes and platelets in the inflamed murine colon. Gastroenterology 132(3):955-65. [PubMed: 17324402]  [MGI Ref ID J:128219]

Vowinkel T; Wood KC; Stokes KY; Russell J; Krieglstein CF; Granger DN. 2006. Differential expression and regulation of murine CD40 in regional vascular beds. Am J Physiol Heart Circ Physiol 290(2):H631-9. [PubMed: 16172156]  [MGI Ref ID J:106724]

Vu F; Dianzani U; Ware CF; Mak T; Gommerman JL. 2008. ICOS, CD40, and lymphotoxin beta receptors signal sequentially and interdependently to initiate a germinal center reaction. J Immunol 180(4):2284-93. [PubMed: 18250437]  [MGI Ref ID J:131998]

Whitmire JK; Flavell RA; Grewal IS; Larsen CP; Pearson TC; Ahmed R. 1999. CD40-CD40 ligand costimulation is required for generating antiviral CD4 T cell responses but is dispensable for CD8 T cell responses. J Immunol 163(6):3194-201. [PubMed: 10477587]  [MGI Ref ID J:110838]

Williams JA; Sharrow SO; Adams AJ; Hodes RJ. 2002. CD40 ligand functions non-cell autonomously to promote deletion of self-reactive thymocytes. J Immunol 168(6):2759-65. [PubMed: 11884443]  [MGI Ref ID J:126679]

Wu ZQ; Vos Q; Shen Y; Lees A; Wilson SR; Briles DE; Gause WC; Mond JJ; Snapper CM. 1999. In vivo polysaccharide-specific IgG isotype responses to intact Streptococcus pneumoniae are T cell dependent and require CD40- and B7-ligand interactions. J Immunol 163(2):659-67. [PubMed: 10395655]  [MGI Ref ID J:119892]

Wuthrich M; Fisette PL; Filutowicz HI; Klein BS. 2006. Differential requirements of T cell subsets for CD40 costimulation in immunity to Blastomyces dermatitidis. J Immunol 176(9):5538-47. [PubMed: 16622023]  [MGI Ref ID J:131650]

Xu H; Yan J; Huang Y; Chilton PM; Ding C; Schanie CL; Wang L; Ildstad ST. 2008. Costimulatory blockade of CD154-CD40 in combination with T-cell lymphodepletion results in prevention of allogeneic sensitization. Blood 111(6):3266-75. [PubMed: 17827394]  [MGI Ref ID J:132727]

Yang Y; Wilson JM. 1996. CD40 ligand-dependent T cell activation: requirement of B7-CD28 signaling through CD40. Science 273(5283):1862-4. [PubMed: 8791591]  [MGI Ref ID J:113051]

Yoshino M; Yamazaki H; Shultz LD; Hayashi S. 2006. Constant rate of steady-state self-antigen trafficking from skin to regional lymph nodes. Int Immunol 18(11):1541-8. [PubMed: 16966493]  [MGI Ref ID J:114990]

Zhou P; Seder RA. 1998. CD40 ligand is not essential for induction of type 1 cytokine responses or protective immunity after primary or secondary infection with histoplasma capsulatum. J Exp Med 187(8):1315-24. [PubMed: 9547342]  [MGI Ref ID J:118790]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX12

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, these mice may be bred as homozygous females and hemizygous males. The targeted mutation is X-linked. These mice are somewhat immunocompromised, specific pathogen-free (SPF) conditions are recommended.
Diet Information LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Pricing for USA, Canada and Mexico shipping destinations             View   International   Pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $86.00MaleHemizygous for Cd40lgtm1Imx
$86.50FemaleHomozygous for Cd40lgtm1Imx
Pairs /Price*Pair Genotype
$172.50Homozygous for Cd40lgtm1Imx x Hemizygous for Cd40lgtm1Imx
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Pricing for International shipping destinations             View   USA, Canada and Mexico   Pricing
Weeks of AgePrice*GenderGenotypes Provided
Individual Mouse Price $111.80MaleHemizygous for Cd40lgtm1Imx
$112.50FemaleHomozygous for Cd40lgtm1Imx
Pairs /Price*Pair Genotype
$224.30Homozygous for Cd40lgtm1Imx x Hemizygous for Cd40lgtm1Imx
*Price(s) in US dollars ($)

Supply Details

Standard SupplyRepository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.
Supply Notes

Control Information

  Allele   Control
 Cd40lgtm1Imx  000664 C57BL/6J
 
  Considerations for Choosing Controls
  USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
  International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

View JAX® Mice & Services Conditions of Use.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering and Purchasing Information

      Purchasing Information
      JAX® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form


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