Strain Name:

B6.129S2-Alox15tm1Fun/J

Stock Number:

002778

Order this mouse

Availability:

Repository- Live

Homozygous (12/15-LO-deficient) mutant mice develop a myeloproliferative disorder (similar to human chronic myelogenous leukemia) that progresses to transplantable leukemia. These mutant mice may be useful in studying myeloproliferative disorders, chronic myelogenous leukemia, and other cancers.

Description

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S2 via D3-H ES cell line
GenerationN11F?+F29 (11-MAY-11)
Generation Definitions

Description
Homozygous (12/15-LO-deficient) mutant mice are viable and fertile. 12/15-LO-deficient mice develop a myeloproliferative disorder (MPD) (similar to human chronic myelogenous leukemia (CML)) that progresses to transplantable leukemia. Chronic MPD stage homozygotes exhibit increased activation of the phosphatidylinositol 3–kinase (PI3-K) pathway, hyperphosphorylation/decreased nuclear accumulation of the transcription factor interferon consensus sequence binding protein (ICSBP), and increased expression of the oncoprotein Bcl-2; all of which are reversible upon treatment with a PI3-K inhibitor. The evolution of MPD to leukemia is associated with additional regulation of ICSBP at the RNA level. Peritoneal macrophages have altered arachidonic acid metabolism as well as a diminished ability to oxidize low density lipoprotein (LDL) following stimulation. These mutant mice may be useful in studying myeloproliferative disorders, chronic myelogenous leukemia, and other cancers.

Development
Neomycin resistance cassettes were inserted into exon 3. Original background was C57BL/6 x 129Sv. As of April 2007, live mice have been backcrossed to C57BL/6J for 11 generations (see SNP note below).

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, 3 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Alox15tm1Fun allele
014163   NOD.129S2(B6)-Alox15tm1Fun/NadlJ
016148   NOD.Cg-Prkdcscid Alox15tm1Fun/NadlJ
View Strains carrying   Alox15tm1Fun     (2 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Alox15tm1Fun/Alox15tm1Fun

        B6.129S2-Alox15tm1Fun/J
  • mortality/aging
  • premature death
    • increase in death rate of homozygous mice as compared to controls   (MGI Ref ID J:119405)
    • 80% survival at 12 months of age   (MGI Ref ID J:119405)
    • severe anemia is most likely cause of death   (MGI Ref ID J:119405)
  • hematopoietic system phenotype
  • abnormal bone marrow cell number
    • increase in number of myeloid cells and progenitors, as well as megkaryocytes   (MGI Ref ID J:119405)
  • abnormal myeloblast morphology/development
    • at death, cells in bone marrow of homozygous mice consist of greater than 25% myeloblasts   (MGI Ref ID J:119405)
    • increased basophil cell number
      • basophilia observed in asymptomatic and moribund mice   (MGI Ref ID J:119405)
  • abnormal myelopoiesis
    • increase in numbers of myeloid cells in bone marrow, spleen, blood and lymph nodes as compared to control   (MGI Ref ID J:119405)
    • cell cycle analysis indicates an increase in proliferative capacity of splenic myeloid cells and a decrease in the percentage of apoptotic cells   (MGI Ref ID J:119405)
  • abnormal spleen red pulp morphology
    • disrupted compartmentalization of red pulp   (MGI Ref ID J:119405)
    • intermingled spleen red and white pulp
      • moribund mice exhibit complete loss of splenic compartmentalization   (MGI Ref ID J:119405)
  • abnormal spleen white pulp morphology
    • disrupted compartmentalization of white pulp   (MGI Ref ID J:119405)
    • abnormal spleen B cell follicle morphology
      • decrease in number of follicles   (MGI Ref ID J:119405)
    • intermingled spleen red and white pulp
      • moribund mice exhibit complete loss of splenic compartmentalization   (MGI Ref ID J:119405)
  • anemia
    • presumed cause of premature death   (MGI Ref ID J:119405)
  • increased leukocyte cell number
    • blood leukocystosis observed in asymptomatic and moribund mice   (MGI Ref ID J:119405)
    • increased basophil cell number
      • basophilia observed in asymptomatic and moribund mice   (MGI Ref ID J:119405)
  • increased spleen weight
    • progressive splenomegaly is observed after 8 weeks of age   (MGI Ref ID J:119405)
  • immune system phenotype
  • abnormal lymph node morphology
    • although not enlarged, lymph nodes exhibit progressive hypercellularity   (MGI Ref ID J:119405)
    • pseudo-Gaucher cells observed in lymph nodes   (MGI Ref ID J:119405)
    • increase in GR-1+ myeloid cells as compared to controls   (MGI Ref ID J:119405)
  • abnormal myelopoiesis
    • increase in numbers of myeloid cells in bone marrow, spleen, blood and lymph nodes as compared to control   (MGI Ref ID J:119405)
    • cell cycle analysis indicates an increase in proliferative capacity of splenic myeloid cells and a decrease in the percentage of apoptotic cells   (MGI Ref ID J:119405)
  • abnormal spleen red pulp morphology
    • disrupted compartmentalization of red pulp   (MGI Ref ID J:119405)
    • intermingled spleen red and white pulp
      • moribund mice exhibit complete loss of splenic compartmentalization   (MGI Ref ID J:119405)
  • abnormal spleen white pulp morphology
    • disrupted compartmentalization of white pulp   (MGI Ref ID J:119405)
    • abnormal spleen B cell follicle morphology
      • decrease in number of follicles   (MGI Ref ID J:119405)
    • intermingled spleen red and white pulp
      • moribund mice exhibit complete loss of splenic compartmentalization   (MGI Ref ID J:119405)
  • dermatitis
    • myeloid infiltrates observed in skin of moribund mice   (MGI Ref ID J:119405)
  • increased leukocyte cell number
    • blood leukocystosis observed in asymptomatic and moribund mice   (MGI Ref ID J:119405)
    • increased basophil cell number
      • basophilia observed in asymptomatic and moribund mice   (MGI Ref ID J:119405)
  • increased spleen weight
    • progressive splenomegaly is observed after 8 weeks of age   (MGI Ref ID J:119405)
  • tumorigenesis
  • increased chronic myelocytic leukemia incidence
    • mice older than 10 weeks develop a malignant myeloproliferative disease at 100% penetrance that can progress to transplantable leukemia   (MGI Ref ID J:119405)
  • integument phenotype
  • dermatitis
    • myeloid infiltrates observed in skin of moribund mice   (MGI Ref ID J:119405)

Alox15tm1Fun/Alox15tm1Fun

        B6.129S2-Alox15tm1Fun
  • immune system phenotype
  • *normal* immune system phenotype
    • in a model of IgE antigen-dependent passive cutaneous anaphylaxis, mice exhibit normal dye extravasation (a measure of edema)   (MGI Ref ID J:197334)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Alox15tm1Fun/Alox15tm1Fun

        involves: 129/Sv * C57BL/6
  • endocrine/exocrine gland phenotype
  • *normal* endocrine/exocrine gland phenotype
    • no defect detected in pituitary gland, adrenals, and pancreas   (MGI Ref ID J:44778)
  • hematopoietic system phenotype
  • *normal* hematopoietic system phenotype
    • mutants had normal total blood cell counts, normal leukocyte differentials, normal reticulocyte numbers, normal hemoglobin values, and normal hematocrit values   (MGI Ref ID J:44778)
  • immune system phenotype
  • abnormal immune system physiology
    • altered arachadonic acid metabolism in peritoneal macrophages upon calcium ionophore stimulation: undetectable 12-hydroxyeicosatetraenoic acid (12-HETE), trace amounts of 15-hydroxyeicosatetraenoic acid (15-HETE), increased concentrations of 5-hydroxyeicosatetraenoic acid (5-HETE), however no defect detected in the spleen   (MGI Ref ID J:44778)
  • reproductive system phenotype
  • *normal* reproductive system phenotype
    • mutants were fertile   (MGI Ref ID J:44778)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Alox15tm1Fun related

Cardiovascular Research
Atherosclerosis
Vascular Defects
      defective macrophage function

Immunology, Inflammation and Autoimmunity Research
Inflammation

Research Tools
Cancer Research
      Leukemia

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Alox15tm1Fun
Allele Name targeted mutation 1, Colin Funk
Allele Type Targeted (Null/Knockout)
Common Name(s) 12/15-LO KO; 12/15-LO-; 12Lox KO; L-12/15-LOX KO; L-12LO-;
Mutation Made By Colin Funk,   University of Pennsylvania
Strain of Origin129S2/SvPas
ES Cell Line NameD3H
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Alox15, arachidonate 15-lipoxygenase
Chromosome 11
Gene Common Name(s) 12-LO; 12-LOX; 15-LOX; 15-LOX-1; 15LOX-1; Alox12; Alox12l; L-12LO; arachidonate 12-lipoxygenase, leukocyte;
Molecular Note Insertion of a neomycin resistance cassette into exon 3 disrupted the Alox15 gene. Northern blot analyses of resident peritoneal macrophages and of bone marrow-derived macrophages did not detect transcript in homozygous mutant mice. Western blot analysis of resident peritoneal macrophages did not detect ALOX15 in homozygous mutant mice. [MGI Ref ID J:44778]

Genotyping

Genotyping Information

Genotyping Protocols

Alox15tm1Fun STD PCR, Melt Curve Analysis
Alox15tm1Fun STD PCR, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Middleton MK; Zukas AM; Rubinstein T; Jacob M; Zhu P; Zhao L; Blair I; Pure E. 2006. Identification of 12/15-lipoxygenase as a suppressor of myeloproliferative disease. J Exp Med 203(11):2529-40. [PubMed: 17043146]  [MGI Ref ID J:119405]

Sun D; Funk CD. 1996. Disruption of 12/15-lipoxygenase expression in peritoneal macrophages. Enhanced utilization of the 5-lipoxygenase pathway and diminished oxidation of low density lipoprotein. J Biol Chem 271(39):24055-62. [PubMed: 8798642]  [MGI Ref ID J:44778]

Additional References

Emerson MR; LeVine SM. 2004. Experimental allergic encephalomyelitis is exacerbated in mice deficient for 12/15-lipoxygenase or 5-lipoxygenase. Brain Res 1021(1):140-5. [PubMed: 15328042]  [MGI Ref ID J:92011]

Klein RF; Allard J; Avnur Z; Nikolcheva T; Rotstein D; Carlos AS; Shea M; Waters RV; Belknap JK; Peltz G; Orwoll ES. 2004. Regulation of bone mass in mice by the lipoxygenase gene Alox15. Science 303(5655):229-32. [PubMed: 14716014]  [MGI Ref ID J:87403]

Zhao L; Cuff CA; Moss E; Wille U; Cyrus T; Klein EA; Pratico D; Rader DJ; Hunter CA; Pure E; Funk CD. 2002. Selective interleukin-12 synthesis defect in 12/15-lipoxygenase-deficient macrophages associated with reduced atherosclerosis in a mouse model of familial hypercholesterolemia. J Biol Chem 277(38):35350-6. [PubMed: 12122008]  [MGI Ref ID J:79120]

Alox15tm1Fun related

Al-Shabrawey M; Mussell R; Kahook K; Tawfik A; Eladl M; Sarthy V; Nussbaum J; El-Marakby A; Park SY; Gurel Z; Sheibani N; Maddipati KR. 2011. Increased expression and activity of 12-lipoxygenase in oxygen-induced ischemic retinopathy and proliferative diabetic retinopathy: implications in retinal neovascularization. Diabetes 60(2):614-24. [PubMed: 21228311]  [MGI Ref ID J:170640]

Andersson CK; Claesson HE; Rydell-Tormanen K; Swedmark S; Hallgren A; Erjefalt JS. 2008. Mice lacking 12/15-lipoxygenase have attenuated airway allergic inflammation and remodeling. Am J Respir Cell Mol Biol 39(6):648-56. [PubMed: 18511709]  [MGI Ref ID J:156003]

Anning PB; Coles B; Bermudez-Fajardo A; Martin PE; Levison BS; Hazen SL; Funk CD; Kuhn H; O'donnell VB. 2005. Elevated endothelial nitric oxide bioactivity and resistance to Angiotensin-dependent hypertension in 12/15-lipoxygenase knockout mice. Am J Pathol 166(3):653-62. [PubMed: 15743778]  [MGI Ref ID J:96724]

Bhowmick R; Tin Maung NH; Hurley BP; Ghanem EB; Gronert K; McCormick BA; Leong JM. 2013. Systemic disease during Streptococcus pneumoniae acute lung infection requires 12-lipoxygenase-dependent inflammation. J Immunol 191(10):5115-23. [PubMed: 24089193]  [MGI Ref ID J:206341]

Chiang N; Shinohara M; Dalli J; Mirakaj V; Kibi M; Choi AM; Serhan CN. 2013. Inhaled carbon monoxide accelerates resolution of inflammation via unique proresolving mediator-heme oxygenase-1 circuits. J Immunol 190(12):6378-88. [PubMed: 23650615]  [MGI Ref ID J:204758]

Chinnici CM; Yao Y; Ding T; Funk CD; Pratico D. 2005. Absence of 12/15 lipoxygenase reduces brain oxidative stress in apolipoprotein e-deficient mice. Am J Pathol 167(5):1371-7. [PubMed: 16251421]  [MGI Ref ID J:102399]

Chou VP; Holman TR; Manning-Bog AB. 2013. Differential contribution of lipoxygenase isozymes to nigrostriatal vulnerability. Neuroscience 228:73-82. [PubMed: 23079635]  [MGI Ref ID J:194022]

Cyrus T; Pratico D; Zhao L; Witztum JL; Rader DJ; Rokach J; FitzGerald GA; Funk CD. 2001. Absence of 12/15-lipoxygenase expression decreases lipid peroxidation and atherogenesis in apolipoprotein e-deficient mice. Circulation 103(18):2277-82. [PubMed: 11342477]  [MGI Ref ID J:103392]

Cyrus T; Witztum JL; Rader DJ; Tangirala R; Fazio S; Linton MF; Funk CD. 1999. Disruption of the 12/15-lipoxygenase gene diminishes atherosclerosis in apo E-deficient mice [see comments] J Clin Invest 103(11):1597-604. [PubMed: 10359569]  [MGI Ref ID J:55714]

DeCostanzo AJ; Voloshyna I; Rosen ZB; Feinmark SJ; Siegelbaum SA. 2010. 12-Lipoxygenase regulates hippocampal long-term potentiation by modulating L-type Ca2+ channels. J Neurosci 30(5):1822-31. [PubMed: 20130191]  [MGI Ref ID J:157853]

Deliri H; Meller N; Kadakkal A; Malhotra R; Brewster J; Doran AC; Pei H; Oldham SN; Skaflen MD; Garmey JC; McNamara CA. 2011. Increased 12/15-lipoxygenase enhances cell growth, fibronectin deposition, and neointimal formation in response to carotid injury. Arterioscler Thromb Vasc Biol 31(1):110-6. [PubMed: 20947825]  [MGI Ref ID J:184196]

Dioszeghy V; Rosas M; Maskrey BH; Colmont C; Topley N; Chaitidis P; Kuhn H; Jones SA; Taylor PR; O'Donnell VB. 2008. 12/15-Lipoxygenase regulates the inflammatory response to bacterial products in vivo. J Immunol 181(9):6514-24. [PubMed: 18941242]  [MGI Ref ID J:140717]

Emerson MR; LeVine SM. 2004. Experimental allergic encephalomyelitis is exacerbated in mice deficient for 12/15-lipoxygenase or 5-lipoxygenase. Brain Res 1021(1):140-5. [PubMed: 15328042]  [MGI Ref ID J:92011]

Feinmark SJ; Begum R; Tsvetkov E; Goussakov I; Funk CD; Siegelbaum SA; Bolshakov VY. 2003. 12-lipoxygenase metabolites of arachidonic acid mediate metabotropic glutamate receptor-dependent long-term depression at hippocampal CA3-CA1 synapses. J Neurosci 23(36):11427-35. [PubMed: 14673007]  [MGI Ref ID J:86891]

George J; Afek A; Shaish A; Levkovitz H; Bloom N; Cyrus T; Zhao L; Funk CD; Sigal E; Harats D. 2001. 12/15-Lipoxygenase gene disruption attenuates atherogenesis in LDL receptor-deficient mice. Circulation 104(14):1646-50. [PubMed: 11581143]  [MGI Ref ID J:86385]

Green-Mitchell SM; Tersey SA; Cole BK; Ma K; Kuhn NS; Cunningham TD; Maybee NA; Chakrabarti SK; McDuffie M; Taylor-Fishwick DA; Mirmira RG; Nadler JL; Morris MA. 2013. Deletion of 12/15-lipoxygenase alters macrophage and islet function in NOD-Alox15(null) mice, leading to protection against type 1 diabetes development. PLoS One 8(2):e56763. [PubMed: 23437231]  [MGI Ref ID J:197192]

Gronert K; Maheshwari N; Khan N; Hassan IR; Dunn M; Laniado Schwartzman M. 2005. A role for the mouse 12/15-lipoxygenase pathway in promoting epithelial wound healing and host defense. J Biol Chem 280(15):15267-78. [PubMed: 15708862]  [MGI Ref ID J:98733]

Huang JT; Welch JS; Ricote M; Binder CJ; Willson TM; Kelly C; Witztum JL; Funk CD; Conrad D; Glass CK. 1999. Interleukin-4-dependent production of PPAR-gamma ligands in macrophages by 12/15-lipoxygenase. Nature 400(6742):378-82. [PubMed: 10432118]  [MGI Ref ID J:200339]

Isobe Y; Arita M; Matsueda S; Iwamoto R; Fujihara T; Nakanishi H; Taguchi R; Masuda K; Sasaki K; Urabe D; Inoue M; Arai H. 2012. Identification and structure determination of novel anti-inflammatory mediator resolvin E3, 17,18-dihydroxyeicosapentaenoic acid. J Biol Chem 287(13):10525-34. [PubMed: 22275352]  [MGI Ref ID J:184090]

Kanazawa Y; Saito Y; Supriatna Y; Tezuka H; Kotani T; Murata Y; Okazawa H; Ohnishi H; Kinouchi Y; Nojima Y; Ohteki T; Shimosegawa T; Matozaki T. 2010. Role of SIRPalpha in regulation of mucosal immunity in the intestine. Genes Cells 15(12):1189-200. [PubMed: 21040253]  [MGI Ref ID J:186324]

Kang KH; Ling TY; Liou HH; Huang YK; Hour MJ; Liou HC; Fu WM. 2013. Enhancement role of host 12/15-lipoxygenase in melanoma progression. Eur J Cancer 49(12):2747-59. [PubMed: 23664097]  [MGI Ref ID J:199008]

Kayama Y; Minamino T; Toko H; Sakamoto M; Shimizu I; Takahashi H; Okada S; Tateno K; Moriya J; Yokoyama M; Nojima A; Yoshimura M; Egashira K; Aburatani H; Komuro I. 2009. Cardiac 12/15 lipoxygenase-induced inflammation is involved in heart failure. J Exp Med 206(7):1565-74. [PubMed: 19546247]  [MGI Ref ID J:150272]

Kenchegowda S; Bazan NG; Bazan HE. 2011. EGF Stimulates Lipoxin A4 Synthesis and Modulates Repair in Corneal Epithelial Cells through ERK and p38 Activation. Invest Ophthalmol Vis Sci 52(5):2240-9. [PubMed: 21220563]  [MGI Ref ID J:171537]

Kinder M; Thompson JE; Wei C; Shelat SG; Blair IA; Carroll M; Pure E. 2010. Interferon regulatory factor-8-driven myeloid differentiation is regulated by 12/15-lipoxygenase-mediated redox signaling. Exp Hematol 38(11):1036-1046.e1-4. [PubMed: 20647030]  [MGI Ref ID J:169623]

Kinder M; Wei C; Shelat SG; Kundu M; Zhao L; Blair IA; Pure E. 2010. Hematopoietic stem cell function requires 12/15-lipoxygenase-dependent fatty acid metabolism. Blood 115(24):5012-22. [PubMed: 20357242]  [MGI Ref ID J:161545]

Klein RF; Allard J; Avnur Z; Nikolcheva T; Rotstein D; Carlos AS; Shea M; Waters RV; Belknap JK; Peltz G; Orwoll ES. 2004. Regulation of bone mass in mice by the lipoxygenase gene Alox15. Science 303(5655):229-32. [PubMed: 14716014]  [MGI Ref ID J:87403]

Kriska T; Cepura C; Magier D; Siangjong L; Gauthier KM; Campbell WB. 2012. Mice lacking macrophage 12/15-lipoxygenase are resistant to experimental hypertension. Am J Physiol Heart Circ Physiol 302(11):H2428-38. [PubMed: 22467300]  [MGI Ref ID J:186729]

Kronke G; Katzenbeisser J; Uderhardt S; Zaiss MM; Scholtysek C; Schabbauer G; Zarbock A; Koenders MI; Axmann R; Zwerina J; Baenckler HW; van den Berg W; Voll RE; Kuhn H; Joosten LA; Schett G. 2009. 12/15-lipoxygenase counteracts inflammation and tissue damage in arthritis. J Immunol 183(5):3383-9. [PubMed: 19675173]  [MGI Ref ID J:151843]

Kundumani-Sridharan V; Dyukova E; Hansen DE 3rd; Rao GN. 2013. 12/15-Lipoxygenase mediates high-fat diet-induced endothelial tight junction disruption and monocyte transmigration: a new role for 15(S)-hydroxyeicosatetraenoic acid in endothelial cell dysfunction. J Biol Chem 288(22):15830-42. [PubMed: 23589307]  [MGI Ref ID J:199619]

Leedom AJ; Sullivan AB; Dong B; Lau D; Gronert K. 2010. Endogenous LXA4 circuits are determinants of pathological angiogenesis in response to chronic injury. Am J Pathol 176(1):74-84. [PubMed: 20008149]  [MGI Ref ID J:156477]

Li Q; Cheon YP; Kannan A; Shanker S; Bagchi IC; Bagchi MK. 2004. A novel pathway involving progesterone receptor, 12/15-lipoxygenase-derived eicosanoids, and peroxisome proliferator-activated receptor gamma regulates implantation in mice. J Biol Chem 279(12):11570-81. [PubMed: 14688261]  [MGI Ref ID J:88738]

Liu S; Xie Z; Zhao Q; Pang H; Turk J; Calderon L; Su W; Zhao G; Xu H; Gong MC; Guo Z. 2012. Smooth muscle-specific expression of calcium-independent phospholipase A2beta (iPLA2beta) participates in the initiation and early progression of vascular inflammation and neointima formation. J Biol Chem 287(29):24739-53. [PubMed: 22637477]  [MGI Ref ID J:188859]

McDuffie M; Maybee NA; Keller SR; Stevens BK; Garmey JC; Morris MA; Kropf E; Rival C; Ma K; Carter JD; Tersey SA; Nunemaker CS; Nadler JL. 2008. Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes. Diabetes 57(1):199-208. [PubMed: 17940120]  [MGI Ref ID J:132435]

McKee AS; Munks MW; MacLeod MK; Fleenor CJ; Van Rooijen N; Kappler JW; Marrack P. 2009. Alum induces innate immune responses through macrophage and mast cell sensors, but these sensors are not required for alum to act as an adjuvant for specific immunity. J Immunol 183(7):4403-14. [PubMed: 19734227]  [MGI Ref ID J:152787]

Middleton MK; Rubinstein T; Pure E. 2006. Cellular and molecular mechanisms of the selective regulation of IL-12 production by 12/15-lipoxygenase. J Immunol 176(1):265-74. [PubMed: 16365418]  [MGI Ref ID J:126265]

Middleton MK; Zukas AM; Rubinstein T; Kinder M; Wilson EH; Zhu P; Blair IA; Hunter CA; Pure E. 2009. 12/15-lipoxygenase-dependent myeloid production of interleukin-12 is essential for resistance to chronic toxoplasmosis. Infect Immun 77(12):5690-700. [PubMed: 19822654]  [MGI Ref ID J:155469]

Miller YI; Chang MK; Funk CD; Feramisco JR; Witztum JL. 2001. 12/15-lipoxygenase translocation enhances site-specific actin polymerization in macrophages phagocytosing apoptotic cells. J Biol Chem 276(22):19431-9. [PubMed: 11278875]  [MGI Ref ID J:200335]

Morita M; Kuba K; Ichikawa A; Nakayama M; Katahira J; Iwamoto R; Watanebe T; Sakabe S; Daidoji T; Nakamura S; Kadowaki A; Ohto T; Nakanishi H; Taguchi R; Nakaya T; Murakami M; Yoneda Y; Arai H; Kawaoka Y; Penninger JM; Arita M; Imai Y. 2013. The lipid mediator protectin D1 inhibits influenza virus replication and improves severe influenza. Cell 153(1):112-25. [PubMed: 23477864]  [MGI Ref ID J:197259]

Nagelin MH; Srinivasan S; Nadler JL; Hedrick CC. 2009. Murine 12/15-lipoxygenase regulates ATP-binding cassette transporter G1 protein degradation through p38- and JNK2-dependent pathways. J Biol Chem 284(45):31303-14. [PubMed: 19713213]  [MGI Ref ID J:156345]

Paintlia AS; Paintlia MK; Singh I; Singh AK. 2006. IL-4-induced peroxisome proliferator-activated receptor gamma activation inhibits NF-kappaB trans activation in central nervous system (CNS) glial cells and protects oligodendrocyte progenitors under neuroinflammatory disease conditions: implication for CNS-demyelinating diseases. J Immunol 176(7):4385-98. [PubMed: 16547277]  [MGI Ref ID J:129865]

Park HA; Khanna S; Rink C; Gnyawali S; Roy S; Sen CK. 2009. Glutathione disulfide induces neural cell death via a 12-lipoxygenase pathway. Cell Death Differ 16(8):1167-79. [PubMed: 19373248]  [MGI Ref ID J:164179]

Poeckel D; Zemski Berry KA; Murphy RC; Funk CD. 2009. Dual 12/15- and 5-lipoxygenase deficiency in macrophages alters arachidonic acid metabolism and attenuates peritonitis and atherosclerosis in apoe knock-out mice. J Biol Chem 284(31):21077-89. [PubMed: 19509298]  [MGI Ref ID J:153174]

Reddy MA; Kim YS; Lanting L; Natarajan R. 2003. Reduced growth factor responses in vascular smooth muscle cells derived from 12/15-lipoxygenase-deficient mice. Hypertension 41(6):1294-300. [PubMed: 12707289]  [MGI Ref ID J:103032]

Seiler A; Schneider M; Forster H; Roth S; Wirth EK; Culmsee C; Plesnila N; Kremmer E; Radmark O; Wurst W; Bornkamm GW; Schweizer U; Conrad M. 2008. Glutathione peroxidase 4 senses and translates oxidative stress into 12/15-lipoxygenase dependent- and AIF-mediated cell death. Cell Metab 8(3):237-48. [PubMed: 18762024]  [MGI Ref ID J:139668]

Singh NK; Kundumani-Sridharan V; Rao GN. 2011. 12/15-Lipoxygenase gene knockout severely impairs ischemia-induced angiogenesis due to lack of Rac1 farnesylation. Blood 118(20):5701-12. [PubMed: 21841162]  [MGI Ref ID J:178807]

Taketomi Y; Ueno N; Kojima T; Sato H; Murase R; Yamamoto K; Tanaka S; Sakanaka M; Nakamura M; Nishito Y; Kawana M; Kambe N; Ikeda K; Taguchi R; Nakamizo S; Kabashima K; Gelb MH; Arita M; Yokomizo T; Nakamura M; Watanabe K; Hirai H; Nakamura M; Okayama Y;Ra C; Aritake K; Urade Y; Morimoto K; Sugimoto Y; Shimizu T; Narumiya S; Hara S; Murakami M. 2013. Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis. Nat Immunol 14(6):554-63. [PubMed: 23624557]  [MGI Ref ID J:197334]

Taylor AM; Hanchett R; Natarajan R; Hedrick CC; Forrest S; Nadler JL; McNamara CA. 2005. The effects of leukocyte-type 12/15-lipoxygenase on Id3-mediated vascular smooth muscle cell growth. Arterioscler Thromb Vasc Biol 25(10):2069-74. [PubMed: 16037566]  [MGI Ref ID J:135379]

Tomio K; Kawana K; Taguchi A; Isobe Y; Iwamoto R; Yamashita A; Kojima S; Mori M; Nagamatsu T; Arimoto T; Oda K; Osuga Y; Taketani Y; Kang JX; Arai H; Arita M; Kozuma S; Fujii T. 2013. Omega-3 polyunsaturated Fatty acids suppress the cystic lesion formation of peritoneal endometriosis in transgenic mouse models. PLoS One 8(9):e73085. [PubMed: 24039864]  [MGI Ref ID J:205921]

Uderhardt S; Herrmann M; Oskolkova OV; Aschermann S; Bicker W; Ipseiz N; Sarter K; Frey B; Rothe T; Voll R; Nimmerjahn F; Bochkov VN; Schett G; Kronke G. 2012. 12/15-lipoxygenase orchestrates the clearance of apoptotic cells and maintains immunologic tolerance. Immunity 36(5):834-46. [PubMed: 22503541]  [MGI Ref ID J:187327]

Vahsen S; Rakowski K; Ledwig D; Dietze-Schroeder D; Swifka J; Sasson S; Eckel J. 2006. Altered GLUT4 translocation in skeletal muscle of 12/15-lipoxygenase knockout mice. Horm Metab Res 38(6):391-6. [PubMed: 16823721]  [MGI Ref ID J:146952]

Walters CL; Wang BC; Godfrey M; Sun D; Funk CD; Blendy JA. 2003. Augmented responses to morphine and cocaine in mice with a 12-lipoxygenase gene disruption. Psychopharmacology (Berl) 170(2):124-31. [PubMed: 12845410]  [MGI Ref ID J:103877]

Wen Y; Gu J; Vandenhoff GE; Liu X; Nadler JL. 2008. Role of 12/15-lipoxygenase in the expression of MCP-1 in mouse macrophages. Am J Physiol Heart Circ Physiol 294(4):H1933-8. [PubMed: 18296557]  [MGI Ref ID J:135665]

Yan Y; Jiang W; Spinetti T; Tardivel A; Castillo R; Bourquin C; Guarda G; Tian Z; Tschopp J; Zhou R. 2013. Omega-3 fatty acids prevent inflammation and metabolic disorder through inhibition of NLRP3 inflammasome activation. Immunity 38(6):1154-63. [PubMed: 23809162]  [MGI Ref ID J:207575]

Zarbock A; Distasi MR; Smith E; Sanders JM; Kronke G; Harry BL; von Vietinghoff S; Buscher K; Nadler JL; Ley K. 2009. Improved survival and reduced vascular permeability by eliminating or blocking 12/15-lipoxygenase in mouse models of acute lung injury (ALI). J Immunol 183(7):4715-22. [PubMed: 19752233]  [MGI Ref ID J:152766]

Zhao L; Cuff CA; Moss E; Wille U; Cyrus T; Klein EA; Pratico D; Rader DJ; Hunter CA; Pure E; Funk CD. 2002. Selective interleukin-12 synthesis defect in 12/15-lipoxygenase-deficient macrophages associated with reduced atherosclerosis in a mouse model of familial hypercholesterolemia. J Biol Chem 277(38):35350-6. [PubMed: 12122008]  [MGI Ref ID J:79120]

Zhao L; Pratico D; Rader DJ; Funk CD. 2005. 12/15-Lipoxygenase gene disruption and vitamin E administration diminish atherosclerosis and oxidative stress in apolipoprotein E deficient mice through a final common pathway. Prostaglandins Other Lipid Mediat 78(1-4):185-93. [PubMed: 16303615]  [MGI Ref ID J:112785]

van Leyen K; Kim HY; Lee SR; Jin G; Arai K; Lo EH. 2006. Baicalein and 12/15-lipoxygenase in the ischemic brain. Stroke 37(12):3014-8. [PubMed: 17053180]  [MGI Ref ID J:135968]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           AX11

Colony Maintenance

Breeding & HusbandryWhen maintaining a live colony, homozygous mice can be bred. Coat color expected from breeding is black.
Mating SystemHomozygote x Homozygote         (Female x Male)   01-MAR-06
Diet Information LabDiet® 5K52/5K67

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $199.90Female or MaleHomozygous for Alox15tm1Fun  
Price per Pair (US dollars $)Pair Genotype
$399.80Homozygous for Alox15tm1Fun x Homozygous for Alox15tm1Fun  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Live Mice

Price per mouse (US dollars $)GenderGenotypes Provided
Individual Mouse $259.90Female or MaleHomozygous for Alox15tm1Fun  
Price per Pair (US dollars $)Pair Genotype
$519.80Homozygous for Alox15tm1Fun x Homozygous for Alox15tm1Fun  

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Repository-Live.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


(6.6)