Description

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation F?+F19N1F8 (04-DEC-07)   Donating Investigator Michael Greenberg,   Childrens Hospital

Appearance
white-bellied agouti
Related Genotype: A^w/A^w

Description
Mice homozygous for defective E2f1 are viable and fertile. They show thymocyte maturation defects due to a failure of apoptosis, eventually resulting in increased proliferation and increased tumorigenesis. As mutant mice age, they show exocrine gland dysplasia and testicular atrophy. Mutant mice develop a broad spectrum of cancers, although mammary carcinomas were not observed on this genetic background. Mutant mice are also protected from experimental autoimmune encephalomyelitis (EAE). These mice may be useful in studies of apoptosis, cancer, thymocyte development/selection, diabetes, autoimmunity, and multiple sclerosis.

Development
This strain was developed by Dr. Seth Field in the laboratory of Dr. Michael Greenberg at Children's Hospital, Boston, MA. The DNA binding domain and part of the dimerization domain of the E2f1 gene were interrupted by a vector containing the neomycin resistance gene.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

E2f1^tm1Meg/E2f1^tm1Meg

        involves: 129S4/SvJae * C57BL/6

• cellular phenotype
• abnormal cell death (MGI Ref ID J:75726)
  • cell death induced by Myc expression in primary fibroblast cells is severely impaired
  • however, fibroblast cells retain their capacity to respond to other apoptotic signals
• immune system phenotype
• decreased T cell proliferation (MGI Ref ID J:76318)
  • T cell receptor stimulated proliferation is impaired
• hematopoietic system phenotype
• decreased T cell proliferation (MGI Ref ID J:76318)
  • T cell receptor stimulated proliferation is impaired

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

E2f1^tm1Meg/E2f1^tm1Meg

        either: (involves: 129/Sv * 129S4/SvJae) or (involves: 129/Sv * 129S4/SvJae * C57BL/6)

• life span-post-weaning/aging
• premature death (MGI Ref ID J:33100)
  • as null animals age, they die at a significantly higher rate than wild-type littermates
• growth/size phenotype
• decreased body weight (MGI Ref ID J:33100)
  • nulls weigh 17% less than wild-type littermates for at least the first 8 weeks of life
• cellular phenotype
• decreased apoptosis (MGI Ref ID J:33100)
  • thymocytes from null mice show increased vialbility in culture with 0% viability over 24 hours compared with 40% viability of wild-type thymocytes
• immune system phenotype
• abnormal double-positive T cell morphology (MGI Ref ID J:33100)
  • double positive cells from null mice display clear enhancement of survival in culture compared to wild-type double-positive cells; in vivo, thymocytes from nulls show significantly less CD3epsilon induced apoptosis compared to wild-type
• enlarged thymus (MGI Ref ID J:33100)
  • in 4 to 6 week old null animals, thymi were noticeably enlarged compared to wild-type
• increased thymus weight (MGI Ref ID J:33100)
  • the ratio of thymus:body weight is 25% higher in nulls relative to wild-type
• increased CD4-positive T cell number (MGI Ref ID J:33100)
  • thymuses of 4-6 week old null mice contain more mature CD4+ cells than wild-type
• increased CD8-positive T cell number (MGI Ref ID J:33100)
  • thymuses of 4-6 week old null mice contain more mature CD8+ cells than wild-type
• increased thymocyte number (MGI Ref ID J:33100)
  • there is a 55% increase in number of thymocytes per thymus in null mice 4-6 weeks old
• reproductive system phenotype
• testicular atrophy (MGI Ref ID J:33100)
  • in 6-12 month old mice, males show moderate testicular atrophy, characterized by a reduction in testicular weight
• hematopoietic system phenotype
• abnormal double-positive T cell morphology (MGI Ref ID J:33100)
  • double positive cells from null mice display clear enhancement of survival in culture compared to wild-type double-positive cells; in vivo, thymocytes from nulls show significantly less CD3epsilon induced apoptosis compared to wild-type
• enlarged thymus (MGI Ref ID J:33100)
  • in 4 to 6 week old null animals, thymi were noticeably enlarged compared to wild-type
• increased thymus weight (MGI Ref ID J:33100)
  • the ratio of thymus:body weight is 25% higher in nulls relative to wild-type
• increased CD4-positive T cell number (MGI Ref ID J:33100)
  • thymuses of 4-6 week old null mice contain more mature CD4+ cells than wild-type
• increased CD8-positive T cell number (MGI Ref ID J:33100)
  • thymuses of 4-6 week old null mice contain more mature CD8+ cells than wild-type
• increased thymocyte number (MGI Ref ID J:33100)
  • there is a 55% increase in number of thymocytes per thymus in null mice 4-6 weeks old
• endocrine/exocrine gland phenotype
• decreased salivation (MGI Ref ID J:93708)
  • E2f1 knockouts have reduced salivary flow rates than NOD controls or the standard (NOD x B10.D2) F1 mice
• testicular atrophy (MGI Ref ID J:33100)
  • in 6-12 month old mice, males show moderate testicular atrophy, characterized by a reduction in testicular weight
• tumorigenesis
• thymic lymphoma (MGI Ref ID J:33100)
  • one 6-12 month old animal exhibited a lymphoblastic lymphoma
• digestive/alimentary phenotype
• decreased salivation (MGI Ref ID J:93708)
  • E2f1 knockouts have reduced salivary flow rates than NOD controls or the standard (NOD x B10.D2) F1 mice
• hearing/vestibular/ear phenotype
• *normal* hearing/vestibular/ear phenotype (MGI Ref ID J:98518)
  • at E18.5, homozygotes display a normal morphology of inner ear sensory epithelia relative to wild-type mice

E2f1^tm1Meg/E2f1^tm1Meg

        involves: 129 * C57BL/6 * FVB/N * NMRI

• vision/eye phenotype
• abnormal cone electrophysiology (MGI Ref ID J:124204)
  • photopic response is slightly reduced relative to normal
• abnormal retinal bipolar cell morphology (MGI Ref ID J:124204)
  • mice have slightly fewer bipolar cells at P18 or P30
  • however, the proportion of bipolar cells is normal
• abnormal retinal progenitor morphology (MGI Ref ID J:124204)
  • retinal progenitor cell proliferation is decreased 2-fold
• decreased retinal ganglion cell number (MGI Ref ID J:124204)
  • mice have slightly fewer ganglion cells at P0
• thin retinal outer nuclear layer (MGI Ref ID J:124204)
  • the retinal outer nuclear layer is slightly reduced in thickness at P18 or P30
• nervous system phenotype
• abnormal retinal bipolar cell morphology (MGI Ref ID J:124204)
  • mice have slightly fewer bipolar cells at P18 or P30
  • however, the proportion of bipolar cells is normal
• decreased retinal ganglion cell number (MGI Ref ID J:124204)
  • mice have slightly fewer ganglion cells at P0

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Apoptosis Research

Cancer Research

Diabetes and Obesity Research

Immunology and Inflammation Research
Autoimmunity (experimental allergic encephalomyelitis (EAE))

Internal/Organ Research
Wound Healing (delayed/impaired)

E2f1^tm1Meg related

Apoptosis Research
Endogenous Regulators

Cancer Research
Increased Tumor Incidence (Lymphomas)
Increased Tumor Incidence (Other Tissues/Organs: multiple)
Tumor Suppressor Genes

Immunology and Inflammation Research
Intracellular Signaling Molecules

Genes & Alleles

Gene & Allele Information

Allele Symbol		E2f1tm1Meg
Allele Name		targeted mutation 1, Michael E Greenburg
Allele Type		Targeted (knock-out)
Common Name(s)		E2F-1-; E2f1-;
Mutation Made By		Seth Field,   Harvard Medical School
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		E2f1, E2F transcription factor 1
Chromosome		2
Gene Common Name(s)		E2F-1; RBAP1; RBBP3; RBP3;
Molecular Note		Part of exon 3, intron 3 and exon 4 were replaced with a neomycin cassette. These sequences encode residues encoding the leucine zipper and those critical for DNA binding. Northern blot analysis did not detect a transcript made from this allele in fibroblasts isolated from homozygous embryos. [MGI Ref ID J:33100]

Genotyping

Genotyping Information

Genotyping Protocols

E2f1^tm1Meg, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Field SJ; Tsai FY; Kuo F; Zubiaga AM; Kaelin WG Jr; Livingston DM; Orkin SH; Greenberg ME. 1996. E2F-1 functions in mice to promote apoptosis and suppress proliferation. Cell 85(4):549-61. [PubMed: 8653790]  [MGI Ref ID J:33100]

Additional References

D'Souza SJ; Vespa A; Murkherjee S; Maher A; Pajak A; Dagnino L. 2002. E2F-1 Is Essential for Normal Epidermal Wound Repair. J Biol Chem 277(12):10626-32. [PubMed: 11790795]  [MGI Ref ID J:75467]

Gao X; Tewari K; Svaren J; Suresh M. 2004. Role of cell cycle regulator E2F1 in regulating CD8 T cell responses during acute and chronic viral infection. Virology 324(2):567-76. [PubMed: 15207641]  [MGI Ref ID J:102273]

Hou ST; Callaghan D; Fournier MC; Hill I; Kang L; Massie B; Morley P; Murray C; Rasquinha I; Slack R; MacManus JP. 2000. The transcription factor E2F1 modulates apoptosis of neurons. J Neurochem 75(1):91-100. [PubMed: 10854251]  [MGI Ref ID J:62798]

Hou ST; Xie X; Baggley A; Park DS; Chen G; Walker T. 2002. Activation of the Rb/E2F1 Pathway by the Nonproliferative p38 MAPK during Fas (APO1/CD95)-mediated Neuronal Apoptosis. J Biol Chem 277(50):48764-70. [PubMed: 12351630]  [MGI Ref ID J:80700]

Iglesias A; Murga M; Laresgoiti U; Skoudy A; Bernales I; Fullaondo A; Moreno B; Lloreta J; Field SJ; Real FX; Zubiaga AM. 2004. Diabetes and exocrine pancreatic insufficiency in E2F1/E2F2 double-mutant mice. J Clin Invest 113(10):1398-407. [PubMed: 15146237]  [MGI Ref ID J:91105]

Iglesias AH; Camelo S; Hwang D; Villanueva R; Stephanopoulos G; Dangond F. 2004. Microarray detection of E2F pathway activation and other targets in multiple sclerosis peripheral blood mononuclear cells. J Neuroimmunol 150(1-2):163-77. [PubMed: 15081262]  [MGI Ref ID J:110246]

Leone G; Sears R; Huang E; Rempel R; Nuckolls F; Park CH; Giangrande P; Wu L; Saavedra HI; Field SJ; Thompson MA; Yang H; Fujiwara Y; Greenberg ME; Orkin S; Smith C; Nevins JR. 2001. Myc requires distinct E2F activities to induce S phase and apoptosis. Mol Cell 8(1):105-13. [PubMed: 11511364]  [MGI Ref ID J:75726]

Li FX; Zhu JW; Tessem JS; Beilke J; Varella-Garcia M; Jensen J; Hogan CJ; DeGregori J. 2003. The development of diabetes in E2f1/E2f2 mutant mice reveals important roles for bone marrow-derived cells in preventing islet cell loss. Proc Natl Acad Sci U S A 100(22):12935-40. [PubMed: 14566047]  [MGI Ref ID J:86404]

Matsumoto N; Salam MA; Watanabe H; Amagasa T; Senpuku H. 2004. Role of gene E2f1 in susceptibility to bacterial adherence of oral streptococci to tooth surfaces in mice. Oral Microbiol Immunol 19(4):270-6. [PubMed: 15209999]  [MGI Ref ID J:110247]

Smith RA; Walker T; Xie X; Hou ST. 2003. Involvement of the transcription factor E2F1/Rb in kainic acid-induced death of murine cerebellar granule cells. Brain Res Mol Brain Res 116(1-2):70-9. [PubMed: 12941462]  [MGI Ref ID J:85607]

Wu L; Timmers C; Maiti B; Saavedra HI; Sang L; Chong GT; Nuckolls F; Giangrande P; Wright FA; Field SJ; Greenberg ME; Orkin S; Nevins JR; Robinson ML; Leone G. 2001. The E2F1-3 transcription factors are essential for cellular proliferation. Nature 414(6862):457-62. [PubMed: 11719808]  [MGI Ref ID J:73374]

Zhu JW; Field SJ; Gore L; Thompson M; Yang H; Fujiwara Y; Cardiff RD; Greenberg M; Orkin SH; DeGregori J. 2001. E2F1 and E2F2 Determine Thresholds for Antigen-Induced T-Cell Proliferation and Suppress Tumorigenesis. Mol Cell Biol 21(24):8547-64. [PubMed: 11713289]  [MGI Ref ID J:72952]

E2f1^tm1Meg related

Andrechek ER; Mori S; Rempel RE; Chang JT; Nevins JR. 2008. Patterns of cell signaling pathway activation that characterize mammary development. Development 135(14):2403-13. [PubMed: 18550711]  [MGI Ref ID J:137644]

Bilousova G; Marusyk A; Porter CC; Cardiff RD; DeGregori J. 2005. Impaired DNA replication within progenitor cell pools promotes leukemogenesis. PLoS Biol 3(12):e401. [PubMed: 16277552]  [MGI Ref ID J:103814]

Cao Q; Xia Y; Azadniv M; Crispe IN. 2004. The E2F-1 transcription factor promotes caspase-8 and bid expression, and enhances Fas signaling in T cells. J Immunol 173(2):1111-7. [PubMed: 15240700]  [MGI Ref ID J:91930]

Chen D; Opavsky R; Pacal M; Tanimoto N; Wenzel P; Seeliger MW; Leone G; Bremner R. 2007. Rb-Mediated Neuronal Differentiation through Cell-Cycle-Independent Regulation of E2f3a. PLoS Biol 5(7):e179. [PubMed: 17608565]  [MGI Ref ID J:124204]

Cooper-Kuhn CM; Vroemen M; Brown J; Ye H; Thompson MA; Winkler J; Kuhn HG. 2002. Impaired adult neurogenesis in mice lacking the transcription factor E2F1. Mol Cell Neurosci 21(2):312-23. [PubMed: 12401450]  [MGI Ref ID J:80102]

DeRyckere D; DeGregori J. 2005. E2F1 and E2F2 are differentially required for homeostasis-driven and antigen-induced T cell proliferation in vivo. J Immunol 175(2):647-55. [PubMed: 16002659]  [MGI Ref ID J:101673]

Fedele M; Visone R; De Martino I; Troncone G; Palmieri D; Battista S; Ciarmiello A; Pallante P; Arra C; Melillo RM; Helin K; Croce CM; Fusco A. 2006. HMGA2 induces pituitary tumorigenesis by enhancing E2F1 activity. Cancer Cell 9(6):459-71. [PubMed: 16766265]  [MGI Ref ID J:110132]

Garcia I; Murga M; Vicario A; Field SJ; Zubiaga AM. 2000. A role for E2F1 in the induction of apoptosis during thymic negative selection. Cell Growth Differ 11(2):91-8. [PubMed: 10714765]  [MGI Ref ID J:133835]

Halaban R; Cheng E; Zhang Y; Mandigo CE; Miglarese MR. 1998. Release of cell cycle constraints in mouse melanocytes by overexpressed mutant E2F1E132, but not by deletion of p16INK4A or p21WAF1/CIP1 Oncogene 16(19):2489-501. [PubMed: 9627115]  [MGI Ref ID J:47889]

Hoglinger GU; Breunig JJ; Depboylu C; Rouaux C; Michel PP; Alvarez-Fischer D; Boutillier AL; Degregori J; Oertel WH; Rakic P; Hirsch EC; Hunot S. 2007. The pRb/E2F cell-cycle pathway mediates cell death in Parkinson's disease. Proc Natl Acad Sci U S A 104(9):3585-90. [PubMed: 17360686]  [MGI Ref ID J:126079]

Hou ST; Callaghan D; Fournier MC; Hill I; Kang L; Massie B; Morley P; Murray C; Rasquinha I; Slack R; MacManus JP. 2000. The transcription factor E2F1 modulates apoptosis of neurons. J Neurochem 75(1):91-100. [PubMed: 10854251]  [MGI Ref ID J:62798]

Hsieh MC; Das D; Sambandam N; Zhang MQ; Nahle Z. 2008. Regulation of the PDK4 isozyme by the Rb-E2F1 complex. J Biol Chem 283(41):27410-7. [PubMed: 18667418]  [MGI Ref ID J:142320]

Iglesias A; Murga M; Laresgoiti U; Skoudy A; Bernales I; Fullaondo A; Moreno B; Lloreta J; Field SJ; Real FX; Zubiaga AM. 2004. Diabetes and exocrine pancreatic insufficiency in E2F1/E2F2 double-mutant mice. J Clin Invest 113(10):1398-407. [PubMed: 15146237]  [MGI Ref ID J:91105]

Ishii H; Mimori K; Yoshikawa Y; Mori M; Furukawa Y; Vecchione A. 2005. Differential Roles of E-Type Cyclins During Transformation of Murine E2F-1-Deficient Cells. DNA Cell Biol 24(3):173-9. [PubMed: 15767783]  [MGI Ref ID J:97842]

Ishimaru N; Arakaki R; Omotehara F; Yamada K; Mishima K; Saito I; Hayashi Y. 2006. Novel role for RbAp48 in tissue-specific, estrogen deficiency-dependent apoptosis in the exocrine glands. Mol Cell Biol 26(8):2924-35. [PubMed: 16581768]  [MGI Ref ID J:107385]

Jiang SX; Sheldrick M; Desbois A; Slinn J; Hou ST. 2007. Neuropilin-1 is a direct target of the transcription factor E2F1 during cerebral ischemia-induced neuronal death in vivo. Mol Cell Biol 27(5):1696-705. [PubMed: 17178835]  [MGI Ref ID J:118900]

Jiang Z; Liang P; Leng R; Guo Z; Liu Y; Liu X; Bubnic S; Keating A; Murray D; Goss P; Zacksenhaus E. 2000. E2F1 and p53 are dispensable, whereas p21(Waf1/Cip1) cooperates with Rb to restrict endoreduplication and apoptosis during skeletal myogenesis Dev Biol 227(1):28-41. [PubMed: 11076674]  [MGI Ref ID J:65679]

Kim TH; Goodman J; Anderson KV; Niswander L. 2007. Phactr4 regulates neural tube and optic fissure closure by controlling PP1-, Rb-, and E2F1-regulated cell-cycle progression. Dev Cell 13(1):87-102. [PubMed: 17609112]  [MGI Ref ID J:123924]

Leone G; Sears R; Huang E; Rempel R; Nuckolls F; Park CH; Giangrande P; Wu L; Saavedra HI; Field SJ; Thompson MA; Yang H; Fujiwara Y; Greenberg ME; Orkin S; Smith C; Nevins JR. 2001. Myc requires distinct E2F activities to induce S phase and apoptosis. Mol Cell 8(1):105-13. [PubMed: 11511364]  [MGI Ref ID J:75726]

Li FX; Zhu JW; Hogan CJ; DeGregori J. 2003. Defective gene expression, S phase progression, and maturation during hematopoiesis in E2F1/E2F2 mutant mice. Mol Cell Biol 23(10):3607-22. [PubMed: 12724419]  [MGI Ref ID J:83284]

Li FX; Zhu JW; Tessem JS; Beilke J; Varella-Garcia M; Jensen J; Hogan CJ; DeGregori J. 2003. The development of diabetes in E2f1/E2f2 mutant mice reveals important roles for bone marrow-derived cells in preventing islet cell loss. Proc Natl Acad Sci U S A 100(22):12935-40. [PubMed: 14566047]  [MGI Ref ID J:86404]

Liu Y; Zacksenhaus E. 2000. E2F1 mediates ectopic proliferation and stage-specific p53-dependent apoptosis but not aberrant differentiation in the ocular lens of Rb deficient fetuses. Oncogene 19(52):6065-73. [PubMed: 11146559]  [MGI Ref ID J:66392]

MacManus JP; Koch CJ; Jian M; Walker T; Zurakowski B. 1999. Decreased brain infarct following focal ischemia in mice lacking the transcription factor E2F1. Neuroreport 10(13):2711-4. [PubMed: 10511428]  [MGI Ref ID J:59752]

Mantela J; Jiang Z; Ylikoski J; Fritzsch B; Zacksenhaus E; Pirvola U. 2005. The retinoblastoma gene pathway regulates the postmitotic state of hair cells of the mouse inner ear. Development 132(10):2377-88. [PubMed: 15843406]  [MGI Ref ID J:98518]

McClellan KA; Ruzhynsky VA; Douda DN; Vanderluit JL; Ferguson KL; Chen D; Bremner R; Park DS; Leone G; Slack RS. 2007. Unique requirement for Rb/E2F3 in neuronal migration: evidence for cell cycle-independent functions. Mol Cell Biol 27(13):4825-43. [PubMed: 17452454]  [MGI Ref ID J:122733]

Murga M; Fernandez-Capetillo O; Field SJ; Moreno B; Borlado LR; Fujiwara Y; Balomenos D; Vicario A; Carrera AC; Orkin SH; Greenberg ME; Zubiaga AM. 2001. Mutation of E2F2 in mice causes enhanced T lymphocyte proliferation, leading to the development of autoimmunity. Immunity 15(6):959-70. [PubMed: 11754817]  [MGI Ref ID J:76318]

Nemajerova A; Mena P; Fingerle-Rowson G; Moll UM; Petrenko O. 2007. Impaired DNA damage checkpoint response in MIF-deficient mice. EMBO J 26(4):987-97. [PubMed: 17290223]  [MGI Ref ID J:120099]

Opavsky R; Tsai SY; Guimond M; Arora A; Opavska J; Becknell B; Kaufmann M; Walton NA; Stephens JA; Fernandez SA; Muthusamy N; Felsher DW; Porcu P; Caligiuri MA; Leone G. 2007. Specific tumor suppressor function for E2F2 in Myc-induced T cell lymphomagenesis. Proc Natl Acad Sci U S A 104(39):15400-5. [PubMed: 17881568]  [MGI Ref ID J:125304]

Palmero I; Murga M; Zubiaga A; Serrano M. 2002. Activation of ARF by oncogenic stress in mouse fibroblasts is independent of E2F1 and E2F2. Oncogene 21(19):2939-47. [PubMed: 12082524]  [MGI Ref ID J:126187]

Qin G; Kishore R; Dolan CM; Silver M; Wecker A; Luedemann CN; Thorne T; Hanley A; Curry C; Heyd L; Dinesh D; Kearney M; Martelli F; Murayama T; Goukassian DA; Zhu Y; Losordo DW. 2006. Cell cycle regulator E2F1 modulates angiogenesis via p53-dependent transcriptional control of VEGF. Proc Natl Acad Sci U S A 103(29):11015-20. [PubMed: 16835303]  [MGI Ref ID J:111802]

Redmond WL; Wei CH; Kreuwel HT; Sherman LA. 2008. The apoptotic pathway contributing to the deletion of naive CD8 T cells during the induction of peripheral tolerance to a cross-presented self-antigen. J Immunol 180(8):5275-82. [PubMed: 18390708]  [MGI Ref ID J:134242]

Saenz-Robles MT; Markovics JA; Chong JL; Opavsky R; Whitehead RH; Leone G; Pipas JM. 2007. Intestinal hyperplasia induced by simian virus 40 large tumor antigen requires E2F2. J Virol 81(23):13191-9. [PubMed: 17855529]  [MGI Ref ID J:129937]

Salam MA; Matin K; Matsumoto N; Tsuha Y; Hanada N; Senpuku H. 2004. E2f1 mutation induces early onset of diabetes and Sjogren's syndrome in nonobese diabetic mice. J Immunol 173(8):4908-18. [PubMed: 15470032]  [MGI Ref ID J:93708]

Sharma N; Timmers C; Trikha P; Saavedra HI; Obery A; Leone G. 2006. Control of the p53-p21CIP1 Axis by E2f1, E2f2, and E2f3 is essential for G1/S progression and cellular transformation. J Biol Chem 281(47):36124-31. [PubMed: 17008321]  [MGI Ref ID J:117634]

Tessem JS; Jensen JN; Pelli H; Dai XM; Zong XH; Stanley ER; Jensen J; DeGregori J. 2008. Critical roles for macrophages in islet angiogenesis and maintenance during pancreatic degeneration. Diabetes 57(6):1605-17. [PubMed: 18375440]  [MGI Ref ID J:136898]

Tsai SY; Opavsky R; Sharma N; Wu L; Naidu S; Nolan E; Feria-Arias E; Timmers C; Opavska J; de Bruin A; Chong JL; Trikha P; Fernandez SA; Stromberg P; Rosol TJ; Leone G. 2008. Mouse development with a single E2F activator. Nature 454(7208):1137-41. [PubMed: 18594513]  [MGI Ref ID J:138289]

Wang ZM; Yang H; Livingston DM. 1998. Endogenous E2F-1 promotes timely G0 exit of resting mouse embryo fibroblasts. Proc Natl Acad Sci U S A 95(26):15583-6. [PubMed: 9861012]  [MGI Ref ID J:119885]

Wikonkal NM; Remenyik E; Knezevic D; Zhang W; Liu M; Zhao H; Berton TR; Johnson DG; Brash DE. 2003. Inactivating E2f1 reverts apoptosis resistance and cancer sensitivity in Trp53-deficient mice. Nat Cell Biol 5(7):655-60. [PubMed: 12833065]  [MGI Ref ID J:84051]

Wu L; Timmers C; Maiti B; Saavedra HI; Sang L; Chong GT; Nuckolls F; Giangrande P; Wright FA; Field SJ; Greenberg ME; Orkin S; Nevins JR; Robinson ML; Leone G. 2001. The E2F1-3 transcription factors are essential for cellular proliferation. Nature 414(6862):457-62. [PubMed: 11719808]  [MGI Ref ID J:73374]

Yan Z; Choi S; Liu X; Zhang M; Schageman JJ; Lee SY; Hart R; Lin L; Thurmond FA; Williams RS. 2003. Highly coordinated gene regulation in mouse skeletal muscle regeneration. J Biol Chem 278(10):8826-36. [PubMed: 12477723]  [MGI Ref ID J:82190]

Zhang J; Bahi N; Zubiaga AM; Comella JX; Llovera M; Sanchis D. 2007. Developmental silencing and independency from E2F of apoptotic gene expression in postmitotic tissues. FEBS Lett 581(30):5781-6. [PubMed: 18037375]  [MGI Ref ID J:129205]

Zhang J; Cicero SA; Wang L; Romito-Digiacomo RR; Yang Y; Herrup K. 2008. Nuclear localization of Cdk5 is a key determinant in the postmitotic state of neurons. Proc Natl Acad Sci U S A 105(25):8772-7. [PubMed: 18550843]  [MGI Ref ID J:137191]

Zhu JW; DeRyckere D; Li FX; Wan YY; DeGregori J. 1999. A role for E2F1 in the induction of ARF, p53, and apoptosis during thymic negative selection. Cell Growth Differ 10(12):829-38. [PubMed: 10616908]  [MGI Ref ID J:133836]

Zhu JW; Field SJ; Gore L; Thompson M; Yang H; Fujiwara Y; Cardiff RD; Greenberg M; Orkin SH; DeGregori J. 2001. E2F1 and E2F2 Determine Thresholds for Antigen-Induced T-Cell Proliferation and Suppress Tumorigenesis. Mol Cell Biol 21(24):8547-64. [PubMed: 11713289]  [MGI Ref ID J:72952]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB29

Colony Maintenance

Breeding & Husbandry	This strain is currently on a mixed C57BL/6 x 129 genetic backgrounds. Litter sizes are reported to be 5-6 pups for these mixed background mice. Homozygotes viable and fertile. Expected coat color from breeding:White Bellied Agouti
Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

OncoMouse^® requires a license from DuPont, see Licenses for Strains with OncoMouse^® Technology.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.