Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Mala Mahendroo,   Univ of Texas Southwest Med Ctr Dallas

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Srd5a1^tm1Mahe/Srd5a1^tm1Mahe

        involves: 129S7/SvEvBrd * C57BL/6J

• reproductive system phenotype
• abnormal parturition (MGI Ref ID J:32217)
  • 33% of homozygous female mice delivered litters on day 19 of gestation; the remainder exhibited prolonged labor, fetal death and often maternal death
• decreased litter size (MGI Ref ID J:32217)
  • homozygous female mice that delivered on day 19 of gestation had an average litter size of 3 pups vs. 8 pups in control females

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Srd5a1^tm1Mahe related

Metabolism Research

Reproductive Biology Research
Developmental Defects Affecting Gonads (defective parturition)
Fertility Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Srd5a1tm1Mahe
Allele Name		targeted mutation 1, Mala Mahendroo
Allele Type		Targeted (knock-out)
Common Name(s)		Srd5a1-;
Mutation Made By		Mala Mahendroo,   Univ of Texas Southwest Med Ctr Dallas
Strain of Origin		129S7/SvEvBrd
ES Cell Line Name		JH1
ES Cell Line Strain		129S7/SvEvBrd
Gene Symbol and Name		Srd5a1, steroid 5 alpha-reductase 1
Chromosome		13
Gene Common Name(s)		0610031P22Rik; 4930435F02Rik; MGC156498; RIKEN cDNA 0610031P22 gene; RIKEN cDNA 4930435F02 gene; Srd5a-1;
Molecular Note		A region encompassing promoter region and the a 3' portion of exon 1, encoding thr first 36 amino acids, was replaced by a neomycin selection cassette. Northern blot analysis of total RNA isolated from several organs showed an absence of transcript in homozygous mutant mice. [MGI Ref ID J:32217]

Genotyping

Genotyping Information

Genotyping Protocols

Srd5a1^tm1Mahe, SEP PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Mahendroo MS; Cala KM; Russell DW. 1996. 5 alpha-reduced androgens play a key role in murine parturition. Mol Endocrinol 10(4):380-92. [PubMed: 8721983]  [MGI Ref ID J:32217]

Additional References

Srd5a1^tm1Mahe related

Frye CA; Walf AA; Rhodes ME; Harney JP. 2004. Progesterone enhances motor, anxiolytic, analgesic, and antidepressive behavior of wild-type mice, but not those deficient in type 1 5 alpha-reductase. Brain Res 1004(1-2):116-24. [PubMed: 15033426]  [MGI Ref ID J:107786]

Mahendroo M; Wilson JD; Richardson JA; Auchus RJ. 2004. Steroid 5alpha-reductase 1 promotes 5alpha-androstane-3alpha,17beta-diol synthesis in immature mouse testes by two pathways. Mol Cell Endocrinol 222(1-2):113-20. [PubMed: 15249131]  [MGI Ref ID J:91120]

Mahendroo MS; Cala KM; Hess DL; Russell DW. 2001. Unexpected virilization in male mice lacking steroid 5alpha-reductase enzymes. Endocrinology 142(11):4652-62. [PubMed: 11606430]  [MGI Ref ID J:72807]

Mahendroo MS; Cala KM; Landrum DP; Russell DW. 1997. Fetal death in mice lacking 5alpha-reductase type 1 caused by estrogen excess. Mol Endocrinol 11(7):917-27. [PubMed: 9178751]  [MGI Ref ID J:40618]

Mahendroo MS; Porter A; Russell DW; Word RA. 1999. The parturition defect in steroid 5alpha-reductase type 1 knockout mice is due to impaired cervical ripening. Mol Endocrinol 13(6):981-92. [PubMed: 10379896]  [MGI Ref ID J:55392]

Timmons BC; Mahendroo MS. 2006. Timing of neutrophil activation and expression of proinflammatory markers do not support a role for neutrophils in cervical ripening in the mouse. Biol Reprod 74(2):236-45. [PubMed: 16237151]  [MGI Ref ID J:105378]

Timmons BC; Mitchell SM; Gilpin C; Mahendroo MS. 2007. Dynamic changes in the cervical epithelial tight junction complex and differentiation occur during cervical ripening and parturition. Endocrinology 148(3):1278-87. [PubMed: 17138657]  [MGI Ref ID J:129596]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
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Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.