Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Species laboratory mouse Background Strain C57BL/6J Donor Strain 129X1 x 129S1 via R1-S3 (+Kitl-SlJ) ES cell line   Donating Investigator IMR Colony,   The Jackson Laboratory

Appearance
black
Related Genotype: a/a

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls

Related Strains

Strains carrying   Notch1^tm1Con allele

002445

STOCK Notch1tm1Con/J

View Strains carrying   Notch1^tm1Con     (1 strain)

Strains carrying other alleles of Notch1

007181	B6.129X1-Notch1tm2Rko/GridJ
006951	STOCK Notch1tm2Rko/GridJ
006953	STOCK Notch1tm3(cre)Rko/J

View Strains carrying other alleles of Notch1     (3 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Notch1^tm1Con/Notch1^tm1Con

        either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)

• lethality-prenatal/perinatal
• embryonic lethality during organogenesis (MGI Ref ID J:25248)
  • most mutants are dead at E10 and all die by E11
• embryogenesis phenotype
• abnormal rostral-caudal axis patterning (MGI Ref ID J:25248)
  • show a deficit in posterior development at E9
• abnormal somite development (MGI Ref ID J:62882)
  • mutants exhibit a lack of coordination in segmentation of the somites, leading to variable somite size and misalignment of the somites across the midline of the embryo
  • transition from presomitic mesoderm to the somite is disordered
  • the most recently formed somites are not as tightly packed as those of wild-type and the epithelialization sometimes appears incomplete
• abnormal left-right axis symmetry of the somites (MGI Ref ID J:25248)
  • lack of coordination across the midline in the segmentation of somites, where segmentation is sometimes present on one side but not the other
• abnormal somite size (MGI Ref ID J:25248)
  • variable somite size
• delayed somite formation (MGI Ref ID J:25248)
  • mutants exhibit a delay in segmentation of the somites
• decreased embryo size (MGI Ref ID J:25248)
  • embryos are smaller at E9
• embryonic growth arrest (MGI Ref ID J:62882)
  • growth arrest at the 14 somite stage
• kinked neural tube (MGI Ref ID J:62882)
  • neural tube kinks are seen extending posterior to the forelimb
• notochord degeneration (MGI Ref ID J:25248)
  • the notochord degenerates after growth arrest
• growth/size phenotype
• decreased embryo size (MGI Ref ID J:25248)
  • embryos are smaller at E9
• cardiovascular system phenotype
• distended pericardium (MGI Ref ID J:62882)
  • distended pericardia at E9
• cellular phenotype
• abnormal cell death (MGI Ref ID J:25248)
  • an increase in cell death is seen in embryos after they arrest
• nervous system phenotype
• kinked neural tube (MGI Ref ID J:62882)
  • neural tube kinks are seen extending posterior to the forelimb

Notch1^tm1Con/Notch1^tm1Con

        involves: 129S1/Sv * 129X1/SvJ

• cardiovascular system phenotype
• abnormal cardiac muscle morphology (MGI Ref ID J:119151)
  • the endocardium does not surround the myocardium, which shows irregular thickness
• disorganized myocardium (MGI Ref ID J:119151)
  • less-structured myocardium
• poorly developed ventricular trabeculae (MGI Ref ID J:119151)
• muscle phenotype
• abnormal cardiac muscle morphology (MGI Ref ID J:119151)
  • the endocardium does not surround the myocardium, which shows irregular thickness
• disorganized myocardium (MGI Ref ID J:119151)
  • less-structured myocardium
• poorly developed ventricular trabeculae (MGI Ref ID J:119151)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Notch1^tm1Con related

Developmental Biology Research
Embryonic Lethality (Homozygous)

Genes & Alleles

Gene & Allele Information

Allele Symbol		Notch1tm1Con
Allele Name		targeted mutation 1, Ronald L Conlon
Allele Type		Targeted (knock-out)
Common Name(s)		N1delta1; Notch1-; Notch1tm1Con/J; Notch-; Notchdelta1;
Mutation Made By		Ronald Conlon,   Case Western Reserve Univ, School of Med
Strain of Origin		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line Name		R1
ES Cell Line Strain		(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name		Notch1, Notch gene homolog 1 (Drosophila)
Chromosome		2
Gene Common Name(s)		9930111A19Rik; Mis6; Motch A; NOTCH; RIKEN cDNA 9930111A19 gene; TAN1; Tan1; hN1; lin-12; translocation-associated Notch;
Molecular Note		Deletion of sequences encoding the EGF repeats 28 to 36, three Notch repeats, the transmembrane domain and CDC10/SWI6 repeats, and replacement with a neomycin gene. [MGI Ref ID J:25248]

Genotyping

Genotyping Information

Genotyping Protocols

Notch1^tm1Con, Standard PCR

Helpful Links

Genotyping resources and troubleshooting

References

References

Selected Reference(s)

Conlon RA; Reaume AG; Rossant J. 1995. Notch1 is required for the coordinate segmentation of somites. Development 121(5):1533-45. [PubMed: 7789282]  [MGI Ref ID J:25248]

Additional References

Notch1^tm1Con related

Barrantes IB; Elia AJ; Wunsch K; De Angelis MH; Mak TW; Rossant J; Conlon RA; Gossler A; de la Pompa JL. 1999. Interaction between Notch signalling and Lunatic fringe during somite boundary formation in the mouse. Curr Biol 9(9):470-80. [PubMed: 10330372]  [MGI Ref ID J:54606]

Cheng HT; Kim M; Valerius MT; Surendran K; Schuster-Gossler K; Gossler A; McMahon AP; Kopan R. 2007. Notch2, but not Notch1, is required for proximal fate acquisition in the mammalian nephron. Development 134(4):801-11. [PubMed: 17229764]  [MGI Ref ID J:119907]

Dale JK; Malapert P; Chal J; Vilhais-Neto G; Maroto M; Johnson T; Jayasinghe S; Trainor P; Herrmann B; Pourquie O. 2006. Oscillations of the snail genes in the presomitic mesoderm coordinate segmental patterning and morphogenesis in vertebrate somitogenesis. Dev Cell 10(3):355-66. [PubMed: 16516838]  [MGI Ref ID J:106622]

Girard L; Jolicoeur P. 1998. A full-length Notch1 allele is dispensable for transformation associated with a provirally activated truncated Notch1 allele in Moloney MuLV-infected MMTV(D)/myc transgenic mice. Oncogene 16(4):517-22. [PubMed: 9484841]  [MGI Ref ID J:45669]

Grego-Bessa J; Luna-Zurita L; del Monte G; Bolos V; Melgar P; Arandilla A; Garratt AN; Zang H; Mukouyama YS; Chen H; Shou W; Ballestar E; Esteller M; Rojas A; Perez-Pomares JM; de la Pompa JL. 2007. Notch signaling is essential for ventricular chamber development. Dev Cell 12(3):415-29. [PubMed: 17336907]  [MGI Ref ID J:119151]

Hadland BK; Huppert SS; Kanungo J; Xue Y; Jiang R; Gridley T; Conlon RA; Cheng AM; Kopan R; Longmore GD. 2004. A requirement for Notch1 distinguishes 2 phases of definitive hematopoiesis during development. Blood 104(10):3097-105. [PubMed: 15251982]  [MGI Ref ID J:94898]

Hitoshi S; Alexson T; Tropepe V; Donoviel D; Elia AJ; Nye JS; Conlon RA; Mak TW; Bernstein A; van Der Kooy D. 2002. Notch pathway molecules are essential for the maintenance, but not the generation, of mammalian neural stem cells. Genes Dev 16(7):846-58. [PubMed: 11937492]  [MGI Ref ID J:75878]

Huppert SS; Ilagan MX; De Strooper B; Kopan R. 2005. Analysis of Notch Function in Presomitic Mesoderm Suggests a gamma-Secretase-Independent Role for Presenilins in Somite Differentiation. Dev Cell 8(5):677-88. [PubMed: 15866159]  [MGI Ref ID J:98438]

Huppert SS; Le A; Schroeter EH; Mumm JS; Saxena MT; Milner LA; Kopan R. 2000. Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1. Nature 405(6789):966-70. [PubMed: 10879540]  [MGI Ref ID J:62882]

Kim YH; Hu H; Guevara-Gallardo S; Lam MT; Fong SY; Wang RA. 2008. Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis. Development 135(22):3755-64. [PubMed: 18952909]  [MGI Ref ID J:144857]

Kumano K; Chiba S; Kunisato A; Sata M; Saito T; Nakagami-Yamaguchi E; Yamaguchi T; Masuda S; Shimizu K; Takahashi T; Ogawa S; Hamada Y; Hirai H. 2003. Notch1 but not Notch2 is essential for generating hematopoietic stem cells from endothelial cells. Immunity 18(5):699-711. [PubMed: 12753746]  [MGI Ref ID J:83475]

Li T; Wen H; Brayton C; Das P; Smithson LA; Fauq A; Fan X; Crain BJ; Price DL; Golde TE; Eberhart CG; Wong PC. 2007. Epidermal growth factor receptor and notch pathways participate in the tumor suppressor function of gamma-secretase. J Biol Chem 282(44):32264-73. [PubMed: 17827153]  [MGI Ref ID J:126817]

Loomes KM; Stevens SA; O'brien ML; Gonzalez DM; Ryan MJ; Segalov M; Dormans NJ; Mimoto MS; Gibson JD; Sewell W; Schaffer AA; Nah HD; Rappaport EF; Pratt SC; Dunwoodie SL; Kusumi K. 2007. Dll3 and Notch1 genetic interactions model axial segmental and craniofacial malformations of human birth defects. Dev Dyn 236(10):2943-51. [PubMed: 17849441]  [MGI Ref ID J:125511]

Nakagawa M; Ichikawa M; Kumano K; Goyama S; Kawazu M; Asai T; Ogawa S; Kurokawa M; Chiba S. 2006. AML1/Runx1 rescues Notch1-null mutation-induced deficiency of para-aortic splanchnopleural hematopoiesis. Blood 108(10):3329-34. [PubMed: 16888092]  [MGI Ref ID J:140445]

Takeshita K; Satoh M; Ii M; Silver M; Limbourg FP; Mukai Y; Rikitake Y; Radtke F; Gridley T; Losordo DW; Liao JK. 2007. Critical role of endothelial Notch1 signaling in postnatal angiogenesis. Circ Res 100(1):70-8. [PubMed: 17158336]  [MGI Ref ID J:130243]

Tan JB; Visan I; Yuan JS; Guidos CJ. 2005. Requirement for Notch1 signals at sequential early stages of intrathymic T cell development. Nat Immunol 6(7):671-9. [PubMed: 15951812]  [MGI Ref ID J:99151]

Thomas JA; Haudek SB; Koroglu T; Tsen MF; Bryant DD; White DJ; Kusewitt DF; Horton JW; Giroir BP. 2003. IRAK1 deletion disrupts cardiac Toll/IL-1 signaling and protects against contractile dysfunction. Am J Physiol Heart Circ Physiol 285(2):H597-606. [PubMed: 12860565]  [MGI Ref ID J:84829]

Timmerman LA; Grego-Bessa J; Raya A; Bertran E; Perez-Pomares JM; Diez J; Aranda S; Palomo S; McCormick F; Izpisua-Belmonte JC; de la Pompa JL. 2004. Notch promotes epithelial-mesenchymal transition during cardiac development and oncogenic transformation. Genes Dev 18(1):99-115. [PubMed: 14701881]  [MGI Ref ID J:87765]

Visan I; Tan JB; Yuan JS; Harper JA; Koch U; Guidos CJ. 2006. Regulation of T lymphopoiesis by Notch1 and Lunatic fringe-mediated competition for intrathymic niches. Nat Immunol 7(6):634-43. [PubMed: 16699526]  [MGI Ref ID J:112675]

de la Pompa JL; Wakeham A; Correia KM; Samper E; Brown S; Aguilera RJ ; Nakano T ; Honjo T ; Mak TW ; Rossant J ; Conlon RA. 1997. Conservation of the Notch signalling pathway in mammalian neurogenesis. Development 124(6):1139-48. [PubMed: 9102301]  [MGI Ref ID J:39351]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 13 and 16 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location). This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Wild-type from the colony
	000664 C57BL/6J
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

See Terms of Use tab for General Terms and Conditions

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice, Products & Services Conditions of Use

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