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- Description
- Disease & phenotype
- Genes & alleles
- Genotyping
- Health & care
- References
- Pricing & purchasing
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Description
Strain Information
Type Deletion; Additional information on Mice with Chromosomal Aberrations. Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Inbred (Female x Male) 11-MAR-11 Species laboratory mouse Generation ?+F18 (28-DEC-04)
Generation DefinitionsAppearance
agouti
Related Genotype: A/A Krd/+
agouti, unaffected
Related Genotype: A/A +/+Important Note
This strain carries a Chromosome 19 deletion that includes: Abcc2, Fgf8, Nkx2-3, Pax2, Scd1, Tlx1, Wnt8b, and Hps1ep. This congenic is homozygous for the wildtype Pde6b+ allele.Description
This is a semidominant, homozygous lethal mutation.Development
(C57BL/6J x C3H)F1 eggs were microinjected with an amylase/rat elastase/chloramphenicol acetyltransferase construct and the founders were bred to C57BL/6J, then to YBR (Johnson et al., 1993). This strain came to The Jackson Laboratory from Dr. Miriam Meisler at the University of Michigan in 1995 as STOCK Tg8052/C3H at N2 to C3H. At The Jackson Laboratory it was bred to C3HeB/FeJ, then one generation to BALB/cByJ. The Krd/+ F1 offspring were then mated with C3.BliA-Pde6b+ (001912) at each generation to produce the congenic C3.BLiA-Pde6b+ -Krd/+ (002802) that we currently offer. The X and Y chromosomes from C3.BliA-Pde6b+ (001912) were captured and fixed in 002802 within the first 3 backcross generations. Since 1995 the Krd/+ breeders were selected primarily by expression of the retinal degeneration phenotype. This strain has passed through only approximately 2 generations per year.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001912 C3A.BLiA-Pde6b+/J | ||
| Considerations for Choosing Controls | ||
Related Strains
Deletion
005535 B6.129S7-Del(11Cops3-Rnf112)1Jrl/J 003374 B6;129S2-H2dlAb1-Ea/J 005654 B6C3-Del(16Cbr1-ORF9)1Rhr/J 004406 C3HeB/FeJ-Pou3f4del-J/J 002142 STOCK 11R30m/J 004711 STOCK Ednrbs-52Pub View Deletion (6 strains)
005252 B6EiC3Sn.BLiA-Ts(1716)65Dn/DnJ 003647 B6EiC3Sn.BLiAF1/J 001912 C3A.BLiA-Pde6b+/J 001979 C3A.Cg-Pde6b+ Prph2Rd2/J 003648 C3Sn.BLiA-Pde6b+/DnJ 004624 FVB.129P2-Pde6b+ Tyrc-ch Fmr1tm1Cgr/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Krd/Krd
involves: C3H/He * C57BL/6 * YBR/Ki
- mortality/aging
- complete embryonic lethality at implantation
- no homozygote embryos are present at E10.5 and the lack of resorbed embryos suggests that lethality occurs before implantation (MGI Ref ID J:20807)
Krd/Krd+
involves: C3H/He * C57BL/6 * YBR/Ki
- mortality/aging
- postnatal lethality
- the expected 50% transgenic offspring are observed at weaning when the founder mice are crossed with C57BL/6 (MGI Ref ID J:20807)
- the percentage of transgenic offspring fall in subsequent backcrosses to C57BL/6 with 35% being transgenic in the 2nd backcross, 19% being transgenic in the 3rd backcross, and 11% being transgenic in the 4th (MGI Ref ID J:20807)
- lethality in the backcrosses to C57BL/6 occurs in the late fetal or early postnatal stage with at least some of the mice dying from kidney aplasia (MGI Ref ID J:20807)
- percentages of transgenic offspring are closer to expected rates when founder mice are crossed with C3H/HeJ mice with 46% being transgenic in the 2nd backcross and 42% being transgenic in the 3rd backcross (MGI Ref ID J:20807)
- growth/size phenotype
- slow postnatal weight gain
- renal/urinary system phenotype
- abnormal kidney corticomedullary boundary morphology
- immature mesenchymal tissue is more prominent at the corticomedullary junction (MGI Ref ID J:20807)
- abnormal nephrogenic zone morphology
- the nephrogenic zone is attenuated and disorganized (MGI Ref ID J:20807)
- absent kidney
- kidneys were absent in one stillborn pup that occurred among 24 observed births (MGI Ref ID J:20807)
- decreased kidney weight
- kidneys with no obvious defects weigh significantly less with a mean of 4.8 mg/g bodyweight compared to 6.2 mg/g for kidneys from littermate controls (MGI Ref ID J:20807)
- decreased nephron number
- the number of mature nephrons is smaller (MGI Ref ID J:20807)
- delayed kidney development
- kidneys from these mice demonstrate considerable immaturity compared with wild-type mice (MGI Ref ID J:20807)
- the nephrogenic zone is attenuated and disorganized (MGI Ref ID J:20807)
- the thickness of the cortical tissue is decreased due in part to less numbers of glomeruli and a smaller profile of proximal convoluted tubules (MGI Ref ID J:20807)
- immature mesenchymal tissue is more prominent at the corticomedullary junction (MGI Ref ID J:20807)
- these immature features are still present at a lesser degree in mice 4 days old but disappear by adulthood (MGI Ref ID J:20807)
- dilated ureter
- is commonly observed (MGI Ref ID J:20807)
- kidney cortex hypoplasia
- the thickness of the cortical tissue is decreased due in part to less numbers of glomeruli and a smaller profile of proximal convoluted tubules (MGI Ref ID J:20807)
- kidney cysts
- unilateral kidney cysts are common in these mice (MGI Ref ID J:20807)
- single kidney
- only one kidney is present in 13% of mice (MGI Ref ID J:20807)
- vision/eye phenotype
- abnormal eye electrophysiology
- abnormal retinal layer morphology
- retinas of some mice are noticeably thinner than controls, with extreme hypocellularity occurring in different positions in the retina (MGI Ref ID J:20807)
- disorganized retinal layers
- all mice contain malformations of the retina (MGI Ref ID J:20807)
- these malformations differed between animals but include zones of narrowing, an excess of somota in a single nuclear layer, photoreceptor nuclei lying ectopically within the photoreceptor layer, and rosettes of photoreceptors enclosed between retinal layers (MGI Ref ID J:20807)
- thin retinal inner nuclear layer
- the nuclear layers are thinner with fewer somata present than in controls (MGI Ref ID J:20807)
- thin retinal outer nuclear layer
- the nuclear layers are thinner with fewer somata present than in controls (MGI Ref ID J:20807)
This mouse can be used to support research in many areas including:Krd related
Pde6b+ relatedInternal/Organ Research
Kidney Defects
Sensorineural Research
Eye Defects
Mouse/Human Gene Homologs
retinitis pigmentosa, wildtype
Sensorineural Research
Retinal Degeneration
wild-type
Genes & Alleles
Gene & Allele Information provided by MGI
| Allele Symbol | Krd | ||
|---|---|---|---|
| Allele Name | kidney and retinal defects | ||
| Allele Type | Transgenic (random, gene disruption) | ||
| Common Name(s) | Tg8052; | ||
| Gene Symbol and Name | Krd, kidney and retinal defects | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | Del(19)TgN8052Mm; | ||
| Molecular Note | The phenotype of this mouse has been attributed to a 7 cM transgene induced deletion, Del(19)TgN8052Mm, which includes the Pax2 and Pkd2l1. The transgene inserted into a LINE element in the distal region of Chromosome 19. The Scd1 and pale ear Hps1 genesare also deleted in Del(19)TgN8052Mm mice, along with several D19Mit markers. [MGI Ref ID J:20807] [MGI Ref ID J:50327] | ||
| Allele Symbol | Pde6b+ | ||
| Allele Name | wild type | ||
| Allele Type | Not Applicable | ||
| Mutation Made By | Frank Kooy, University of Antwerp | ||
| Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CSNB3; CSNBAD2; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10; | ||
References
References provided by MGI
Additional References
Johnson TM; Rosenberg MP; Meisler MH. 1993. An insulin-responsive element in the pancreatic enhancer of the amylase gene. J Biol Chem 268(1):464-8. [PubMed: 7678001] [MGI Ref ID J:30983]
Keller SA; Jones JM; Boyle A; Barrow LL; Killen PD; Green DG; Kapousta NV; Hitchcock PF; Swank RT; Meisler MH. 1994. Kidney and retinal defects (Krd), a transgene-induced mutation with a deletion of mouse chromosome 19 that includes the Pax2 locus. Genomics 23(2):309-20. [PubMed: 7835879] [MGI Ref ID J:20807]
Krd relatedPde6b+ relatedBedell MA; Largaespada DA; Jenkins NA; Copeland NG. 1997. Mouse models of human disease. Part II: recent progress and future directions. Genes Dev 11(1):11-43. [PubMed: 9000048] [MGI Ref ID J:37854]
Hawes NL; Smith RS; Chang B; Davisson M; Heckenlively JR; John SW. 1999. Mouse fundus photography and angiography: a catalogue of normal and mutant phenotypes. Mol Vis 5:22. [PubMed: 10493779] [MGI Ref ID J:59481]
Keller SA; Jones JM; Boyle A; Barrow LL; Killen PD; Green DG; Kapousta NV; Hitchcock PF; Swank RT; Meisler MH. 1994. Kidney and retinal defects (Krd), a transgene-induced mutation with a deletion of mouse chromosome 19 that includes the Pax2 locus. Genomics 23(2):309-20. [PubMed: 7835879] [MGI Ref ID J:20807]
Nomura H; Turco AE; Pei Y; Kalaydjieva L; Schiavello T; Weremowicz S ; Ji W ; Morton CC ; Meisler M ; Reeders ST ; Zhou J. 1998. Identification of PKDL, a novel polycystic kidney disease 2-like gene whose murine homologue is deleted in mice with kidney and retinal defects. J Biol Chem 273(40):25967-73. [PubMed: 9748274] [MGI Ref ID J:50327]
Otteson DC; Shelden E; Jones JM; Kameoka J; Hitchcock PF. 1998. Pax2 expression and retinal morphogenesis in the normal and Krd mouse. Dev Biol 193(2):209-24. [PubMed: 9473325] [MGI Ref ID J:45929]
Urbanek P; Fetka I; Meisler MH; Busslinger M. 1997. Cooperation of Pax2 and Pax5 in midbrain and cerebellum development. Proc Natl Acad Sci U S A 94(11):5703-8. [PubMed: 9159136] [MGI Ref ID J:40910]
Dobkin C; Rabe A; Dumas R; El Idrissi A; Haubenstock H; Brown WT. 2000. Fmr1 knockout mouse has a distinctive strain-specific learning impairment. Neuroscience 100(2):423-9. [PubMed: 11008180] [MGI Ref ID J:119166]
Ivanco TL; Greenough WT. 2002. Altered mossy fiber distributions in adult Fmr1 (FVB) knockout mice. Hippocampus 12(1):47-54. [PubMed: 11918288] [MGI Ref ID J:113177]
Sakamoto K; McCluskey M; Wensel TG; Naggert JK; Nishina PM. 2009. New mouse models for recessive retinitis pigmentosa caused by mutations in the Pde6a gene. Hum Mol Genet 18(1):178-92. [PubMed: 18849587] [MGI Ref ID J:142108]
Zhao MG; Toyoda H; Ko SW; Ding HK; Wu LJ; Zhuo M. 2005. Deficits in trace fear memory and long-term potentiation in a mouse model for fragile X syndrome. J Neurosci 25(32):7385-92. [PubMed: 16093389] [MGI Ref ID J:100197]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number A1
Colony Maintenance
Mating System Heterozygote x Inbred (Female x Male) 11-MAR-11
Pricing and Purchasing
Pricing, Supply Level & Notes, Controls
| Pricing for USA, Canada and Mexico shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Heterozygous for Krd
Price per Pair (US dollars $) Pair Genotype $286.30 Heterozygous for Krd x C3A.BLiA-Pde6b+/J (001912) Standard Supply
Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
| Pricing for International shipping destinations |
|
Live Mice
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Heterozygous for Krd
Price per Pair (US dollars $) Pair Genotype $372.20 Heterozygous for Krd x C3A.BLiA-Pde6b+/J (001912) Standard Supply
Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
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Standard Supply
Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
General Supply Notes
- This strain is included in the Eye Mutant Resource within the Mouse Mutant Resource collection.
- Genomic DNA is available for this strain from the Mouse DNA Resource.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001912 C3A.BLiA-Pde6b+/J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Important Note
This strain carries a Chromosome 19 deletion that includes: Abcc2, Fgf8, Nkx2-3, Pax2, Scd1, Tlx1, Wnt8b, and Hps1ep. This congenic is homozygous for the wildtype Pde6b+ allele.
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The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering Information
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