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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Type Deletion; Additional information on Mice with Chromosomal Aberrations. Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Hemizygote x Inbred (Female x Male) 01-MAR-06 Species laboratory mouse Generation ?+F18 (28-DEC-04) Appearance
agouti
Related Genotype: A/A Krd/+
agouti, unaffected
Related Genotype: A/A +/+Important Note
This strain carries a Chromosome 19 deletion that includes: Abcc2, Fgf8, Nkx2-3, Pax2, Scd1, Tlx1, Wnt8b, and Hps1ep. This congenic is homozygous for the wildtype Pde6b+ allele.Description
This is a semidominant, homozygous lethal mutation.Development
(C57BL/6J x C3H)F1 eggs were microinjected with an amylase/rat elastase/chloramphenicol acetyltransferase construct and the founders were bred to C57BL/6J, then to YBR (Johnson et al., 1993). This strain came to The Jackson Laboratory from Dr. Miriam Meisler at the University of Michigan in 1995 as STOCK Tg8052/C3H at N2 to C3H. At The Jackson Laboratory it was bred to C3HeB/FeJ, then one generation to BALB/cByJ. The Krd/+ F1 offspring were then mated with C3.BliA-Pde6b+ (001912) at each generation to produce the congenic C3.BLiA-Pde6b+ -Krd/+ (002802) that we currently offer. The X and Y chromosomes from C3.BliA-Pde6b+ (001912) were captured and fixed in 002802 within the first 3 backcross generations. Since 1995 the Krd/+ breeders were selected primarily by expression of the retinal degeneration phenotype. This strain has passed through only approximately 2 generations per year.
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001912 C3A.BLiA-Pde6b+/J | ||
| Considerations for Choosing Controls | ||
Related Strains
Deletion
005535 B6.129S7-Del(11Cops3-Zfp179)1Jrl/J 003374 B6;129S2-H2dlAb1-Ea/J 005654 B6C3-Del(16Cbr1-ORF9)1Rhr/J 004406 C3HeB/FeJ-Pou3f4del-J/J 002142 STOCK 11R30m/J 004711 STOCK Ednrbs-52Pub View Deletion (6 strains)
003647 B6EiC3Sn.BLiAF1 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
Phenotype
Phenotype Information
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Krd/Krd
involves: C3H/He * C57BL/6 * YBR/Ki
- lethality-prenatal/perinatal
- embryonic lethality at implantation (MGI Ref ID J:20807)
- no homozygote embryos are present at E10.5 and the lack of resorbed embryos suggests that lethality occurs before implantation
Krd/Krd+
involves: C3H/He * C57BL/6 * YBR/Ki
- lethality-postnatal
- postnatal lethality (MGI Ref ID J:20807)
- the expected 50% transgenic offspring are observed at weaning when the founder mice are crossed with C57BL/6
- the percentage of transgenic offspring fall in subsequent backcrosses to C57BL/6 with 35% being transgenic in the 2nd backcross, 19% being transgenic in the 3rd backcross, and 11% being transgenic in the 4th
- lethality in the backcrosses to C57BL/6 occurs in the late fetal or early postnatal stage with at least some of the mice dying from kidney aplasia
- percentages of transgenic offspring are closer to expected rates when founder mice are crossed with C3H/HeJ mice with 46% being transgenic in the 2nd backcross and 42% being transgenic in the 3rd backcross
- growth/size phenotype
- postnatal slow weight gain (MGI Ref ID J:20807)
- the growth of these mice is retarded for the first 2 months of birth being about 80% of littermate controls
- normal weight is eventually reached
- renal/urinary system phenotype
- abnormal nephron morphology (MGI Ref ID J:20807)
- the number of mature nephrons is smaller
- absent kidney (MGI Ref ID J:20807)
- kidneys were absent in one stillborn pup that occurred among 24 observed births
- decreased kidney weight (MGI Ref ID J:20807)
- kidneys with no obvious defects weigh significantly less with a mean of 4.8 mg/g bodyweight compared to 6.2 mg/g for kidneys from littermate controls
- delayed kidney development (MGI Ref ID J:20807)
- kidneys from these mice demonstrate considerable immaturity compared with wild-type mice
- the nephrogenic zone is attenuated and disorganized
- the thickness of the cortical tissue is decreased due in part to less numbers of glomeruli and a smaller profile of proximal convoluted tubules
- immature mesenchymal tissue is more prominent at the corticomedullary junction
- these immature features are still present at a lesser degree in mice 4 days old but disappear by adulthood
- dilated ureter (MGI Ref ID J:20807)
- is commonly observed
- kidney cysts (MGI Ref ID J:20807)
- unilateral kidney cysts are common in these mice
- single kidney (MGI Ref ID J:20807)
- only one kidney is present in 13% of mice
- vision/eye phenotype
- abnormal eye electrophysiology (MGI Ref ID J:20807)
- the electroretinograms of eight mice varied from three in low normal range to three that are extremely abnormal
- both a- and b- waves are abnormal in mice in affected mice
- abnormal retinal layer morphology (MGI Ref ID J:20807)
- retinas of some mice are noticeably thinner than controls, with extreme hypocellularity occurring in different positions in the retina
- disorganized retinal layers (MGI Ref ID J:20807)
- all mice contain malformations of the retina
- these malformations differed between animals but include zones of narrowing, an excess of somota in a single nuclear layer, photoreceptor nuclei lying ectopically within the photoreceptor layer, and rosettes of photoreceptors enclosed between retinal layers
- thin retinal inner nuclear layer (MGI Ref ID J:20807)
- the nuclear layers are thinner with fewer somata present than in controls
- thin retinal outer nuclear layer (MGI Ref ID J:20807)
- the nuclear layers are thinner with fewer somata present than in controls
This mouse can be used to support research in many areas including:Krd related
Pde6b+ relatedInternal/Organ Research
Kidney Defects
Sensorineural Research
Eye Defects
Mouse/Human Gene Homologs
retinitis pigmentosa, wildtype
Sensorineural Research
Retinal Degeneration
wild-type
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Krd | ||
|---|---|---|---|
| Allele Name | kidney and retinal defects | ||
| Allele Type | Transgenic (random, gene disruption) | ||
| Common Name(s) | Tg8052; | ||
| Gene Symbol and Name | Krd, kidney and retinal defects | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | Del(19)TgN8052Mm; | ||
| Molecular Note | The phenotype of this mouse has been attributed to a 7 cM transgene induced deletion, Del(19)TgN8052Mm, which includes the Pax2 and Pkd2l1. The transgene inserted into a LINE element in the distal region of Chromosome 19. The Scd1 and pale ear Hps1 genesare also deleted in Del(19)TgN8052Mm mice, along with several D19Mit markers. [MGI Ref ID J:20807] [MGI Ref ID J:50327] | ||
| Allele Symbol | Pde6b+ | ||
| Allele Name | wild type | ||
| Allele Type | Not Applicable | ||
| Mutation Made By | Frank Kooy, University of Antwerp | ||
| Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CSNB3; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10; | ||
Genotyping
Genotyping Information
This strain will not have a genotyping protocol or one is not currently available.
Helpful Links
Genotyping resources and troubleshooting
References
References
Additional References
Johnson TM; Rosenberg MP; Meisler MH. 1993. An insulin-responsive element in the pancreatic enhancer of the amylase gene. J Biol Chem 268(1):464-8. [PubMed: 7678001] [MGI Ref ID J:30983]
Keller SA; Jones JM; Boyle A; Barrow LL; Killen PD; Green DG; Kapousta NV; Hitchcock PF; Swank RT; Meisler MH. 1994. Kidney and retinal defects (Krd), a transgene-induced mutation with a deletion of mouse chromosome 19 that includes the Pax2 locus. Genomics 23(2):309-20. [PubMed: 7835879] [MGI Ref ID J:20807]
Krd relatedPde6b+ relatedBedell MA; Largaespada DA; Jenkins NA; Copeland NG. 1997. Mouse models of human disease. Part II: recent progress and future directions. Genes Dev 11(1):11-43. [PubMed: 9000048] [MGI Ref ID J:37854]
Hawes NL; Smith RS; Chang B; Davisson M; Heckenlively JR; John SW. 1999. Mouse fundus photography and angiography: a catalogue of normal and mutant phenotypes. Mol Vis 5:22. [PubMed: 10493779] [MGI Ref ID J:59481]
Keller SA; Jones JM; Boyle A; Barrow LL; Killen PD; Green DG; Kapousta NV; Hitchcock PF; Swank RT; Meisler MH. 1994. Kidney and retinal defects (Krd), a transgene-induced mutation with a deletion of mouse chromosome 19 that includes the Pax2 locus. Genomics 23(2):309-20. [PubMed: 7835879] [MGI Ref ID J:20807]
Nomura H; Turco AE; Pei Y; Kalaydjieva L; Schiavello T; Weremowicz S ; Ji W ; Morton CC ; Meisler M ; Reeders ST ; Zhou J. 1998. Identification of PKDL, a novel polycystic kidney disease 2-like gene whose murine homologue is deleted in mice with kidney and retinal defects. J Biol Chem 273(40):25967-73. [PubMed: 9748274] [MGI Ref ID J:50327]
Otteson DC; Shelden E; Jones JM; Kameoka J; Hitchcock PF. 1998. Pax2 expression and retinal morphogenesis in the normal and Krd mouse. Dev Biol 193(2):209-24. [PubMed: 9473325] [MGI Ref ID J:45929]
Urbanek P; Fetka I; Meisler MH; Busslinger M. 1997. Cooperation of Pax2 and Pax5 in midbrain and cerebellum development. Proc Natl Acad Sci U S A 94(11):5703-8. [PubMed: 9159136] [MGI Ref ID J:40910]
Sakamoto K; McCluskey M; Wensel TG; Naggert JK; Nishina PM. 2009. New mouse models for recessive retinitis pigmentosa caused by mutations in the Pde6a gene. Hum Mol Genet 18(1):178-92. [PubMed: 18849587] [MGI Ref ID J:142108]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number A1
Colony Maintenance
Mating System Hemizygote x Inbred (Female x Male) 01-MAR-06
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $127.90 Female or Male Heterozygous for Krd
Pairs /Price (US dollars $) Pair Genotype $219.20 Heterozygous for Krd x C3A.BLiA-Pde6b<+>/J (001912)
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $166.30 Female or Male Heterozygous for Krd
Pairs /Price (US dollars $) Pair Genotype $285.00 Heterozygous for Krd x C3A.BLiA-Pde6b<+>/J (001912)
Additional Supply Details
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Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
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| Important Note | |
| This strain carries a Chromosome 19 deletion that includes: Abcc2, Fgf8, Nkx2-3, Pax2, Scd1, Tlx1, Wnt8b, and Hps1ep. This congenic is homozygous for the wildtype Pde6b+ allele. | |
Control Information
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001912 C3A.BLiA-Pde6b+/J | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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