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Type Deletion; Additional information on Mice with Chromosomal Aberrations. Type Congenic; Mutant Strain; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Heterozygote x Inbred (Female x Male) 11-MAR-11 Species laboratory mouse Generation ?+F18 (28-DEC-04)
Generation DefinitionsAppearance
agouti
Related Genotype: A/A Krd/+
agouti, unaffected
Related Genotype: A/A +/+Important Note
This strain carries a Chromosome 19 deletion that includes: Abcc2, Fgf8, Nkx2-3, Pax2, Scd1, Tlx1, Wnt8b, and Hps1ep. This congenic is homozygous for the wildtype Pde6b+ allele.Description
This is a semidominant, homozygous lethal mutation.Development
(C57BL/6J x C3H)F1 eggs were microinjected with an amylase/rat elastase/chloramphenicol acetyltransferase construct and the founders were bred to C57BL/6J, then to YBR (Johnson et al., 1993). This strain came to The Jackson Laboratory from Dr. Miriam Meisler at the University of Michigan in 1995 as STOCK Tg8052/C3H at N2 to C3H. At The Jackson Laboratory it was bred to C3HeB/FeJ, then one generation to BALB/cByJ. The Krd/+ F1 offspring were then mated with C3.BliA-Pde6b+ (001912) at each generation to produce the congenic C3.BLiA-Pde6b+ -Krd/+ (002802) that we currently offer. The X and Y chromosomes from C3.BliA-Pde6b+ (001912) were captured and fixed in 002802 within the first 3 backcross generations. Since 1995 the Krd/+ breeders were selected primarily by expression of the retinal degeneration phenotype. This strain has passed through only approximately 2 generations per year.
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001912 C3A.BLiA-Pde6b+/J | ||
| Considerations for Choosing Controls | ||
Deletion
005535 B6.129S7-Del(11Cops3-Rnf112)1Jrl/J 003374 B6;129S2-H2dlAb1-Ea/J 005654 B6C3-Del(16Cbr1-ORF9)1Rhr/J 004406 C3HeB/FeJ-Pou3f4del-J/J 002142 STOCK 11R30m/J 004711 STOCK Ednrbs-52Pub View Deletion (6 strains)
Strains carrying Pde6b+ allele
005252 B6EiC3Sn.BLiA-Ts(1716)65Dn/DnJ 003647 B6EiC3Sn.BLiAF1/J 001912 C3A.BLiA-Pde6b+/J 001979 C3A.Cg-Pde6b+ Prph2Rd2/J 003648 C3Sn.BLiA-Pde6b+/DnJ 004624 FVB.129P2-Pde6b+ Tyrc-ch Fmr1tm1Cgr/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J View Strains carrying Pde6b+ (8 strains)
Strains carrying other alleles of Pde6b
View Strains carrying other alleles of Pde6b (77 strains)
View Mammalian Phenotype Terms
Mammalian Phenotype Terms provided by MGI
assigned by genotype
The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.
Krd/Krd
involves: C3H/He * C57BL/6 * YBR/Ki
- mortality/aging
- complete embryonic lethality at implantation
- no homozygote embryos are present at E10.5 and the lack of resorbed embryos suggests that lethality occurs before implantation (MGI Ref ID J:20807)
Krd/Krd+
involves: C3H/He * C57BL/6 * YBR/Ki
- mortality/aging
- postnatal lethality
- the expected 50% transgenic offspring are observed at weaning when the founder mice are crossed with C57BL/6 (MGI Ref ID J:20807)
- the percentage of transgenic offspring fall in subsequent backcrosses to C57BL/6 with 35% being transgenic in the 2nd backcross, 19% being transgenic in the 3rd backcross, and 11% being transgenic in the 4th (MGI Ref ID J:20807)
- lethality in the backcrosses to C57BL/6 occurs in the late fetal or early postnatal stage with at least some of the mice dying from kidney aplasia (MGI Ref ID J:20807)
- percentages of transgenic offspring are closer to expected rates when founder mice are crossed with C3H/HeJ mice with 46% being transgenic in the 2nd backcross and 42% being transgenic in the 3rd backcross (MGI Ref ID J:20807)
- growth/size phenotype
- slow postnatal weight gain
- renal/urinary system phenotype
- abnormal kidney corticomedullary boundary morphology
- immature mesenchymal tissue is more prominent at the corticomedullary junction (MGI Ref ID J:20807)
- abnormal nephrogenic zone morphology
- the nephrogenic zone is attenuated and disorganized (MGI Ref ID J:20807)
- absent kidney
- kidneys were absent in one stillborn pup that occurred among 24 observed births (MGI Ref ID J:20807)
- decreased kidney weight
- kidneys with no obvious defects weigh significantly less with a mean of 4.8 mg/g bodyweight compared to 6.2 mg/g for kidneys from littermate controls (MGI Ref ID J:20807)
- decreased nephron number
- the number of mature nephrons is smaller (MGI Ref ID J:20807)
- delayed kidney development
- kidneys from these mice demonstrate considerable immaturity compared with wild-type mice (MGI Ref ID J:20807)
- the nephrogenic zone is attenuated and disorganized (MGI Ref ID J:20807)
- the thickness of the cortical tissue is decreased due in part to less numbers of glomeruli and a smaller profile of proximal convoluted tubules (MGI Ref ID J:20807)
- immature mesenchymal tissue is more prominent at the corticomedullary junction (MGI Ref ID J:20807)
- these immature features are still present at a lesser degree in mice 4 days old but disappear by adulthood (MGI Ref ID J:20807)
- dilated ureter
- is commonly observed (MGI Ref ID J:20807)
- kidney cortex hypoplasia
- the thickness of the cortical tissue is decreased due in part to less numbers of glomeruli and a smaller profile of proximal convoluted tubules (MGI Ref ID J:20807)
- kidney cysts
- unilateral kidney cysts are common in these mice (MGI Ref ID J:20807)
- single kidney
- only one kidney is present in 13% of mice (MGI Ref ID J:20807)
- vision/eye phenotype
- abnormal eye electrophysiology
- abnormal retinal layer morphology
- retinas of some mice are noticeably thinner than controls, with extreme hypocellularity occurring in different positions in the retina (MGI Ref ID J:20807)
- disorganized retinal layers
- all mice contain malformations of the retina (MGI Ref ID J:20807)
- these malformations differed between animals but include zones of narrowing, an excess of somota in a single nuclear layer, photoreceptor nuclei lying ectopically within the photoreceptor layer, and rosettes of photoreceptors enclosed between retinal layers (MGI Ref ID J:20807)
- thin retinal inner nuclear layer
- the nuclear layers are thinner with fewer somata present than in controls (MGI Ref ID J:20807)
- thin retinal outer nuclear layer
- the nuclear layers are thinner with fewer somata present than in controls (MGI Ref ID J:20807)
View Research Applications
Research Applications
This mouse can be used to support research in many areas including:Krd related
Pde6b+ relatedInternal/Organ Research
Kidney Defects
Sensorineural Research
Eye Defects
Mouse/Human Gene Homologs
retinitis pigmentosa, wildtype
Sensorineural Research
Retinal Degeneration
wild-type
| Allele Symbol | Krd | ||
|---|---|---|---|
| Allele Name | kidney and retinal defects | ||
| Allele Type | Transgenic (random, gene disruption) | ||
| Common Name(s) | Tg8052; | ||
| Gene Symbol and Name | Krd, kidney and retinal defects | ||
| Chromosome | 19 | ||
| Gene Common Name(s) | Del(19)TgN8052Mm; | ||
| Molecular Note | The phenotype of this mouse has been attributed to a 7 cM transgene induced deletion, Del(19)TgN8052Mm, which includes the Pax2 and Pkd2l1. The transgene inserted into a LINE element in the distal region of Chromosome 19. The Scd1 and pale ear Hps1 genesare also deleted in Del(19)TgN8052Mm mice, along with several D19Mit markers. [MGI Ref ID J:20807] [MGI Ref ID J:50327] | ||
| Allele Symbol | Pde6b+ | ||
| Allele Name | wild type | ||
| Allele Type | Not Applicable | ||
| Mutation Made By | Frank Kooy, University of Antwerp | ||
| Gene Symbol and Name | Pde6b, phosphodiesterase 6B, cGMP, rod receptor, beta polypeptide | ||
| Chromosome | 5 | ||
| Gene Common Name(s) | CSNB3; CSNBAD2; PDEB; Pdeb; RP40; nmf137; phosphodiesterase, cGMP, rod receptor, beta polypeptide; r; rd; rd-1; rd1; rd10; retinal degeneration; retinal degeneration 1; retinal degeneration 10; | ||
Johnson TM; Rosenberg MP; Meisler MH. 1993. An insulin-responsive element in the pancreatic enhancer of the amylase gene. J Biol Chem 268(1):464-8. [PubMed: 7678001] [MGI Ref ID J:30983]
Keller SA; Jones JM; Boyle A; Barrow LL; Killen PD; Green DG; Kapousta NV; Hitchcock PF; Swank RT; Meisler MH. 1994. Kidney and retinal defects (Krd), a transgene-induced mutation with a deletion of mouse chromosome 19 that includes the Pax2 locus. Genomics 23(2):309-20. [PubMed: 7835879] [MGI Ref ID J:20807]
Krd relatedPde6b+ relatedBedell MA; Largaespada DA; Jenkins NA; Copeland NG. 1997. Mouse models of human disease. Part II: recent progress and future directions. Genes Dev 11(1):11-43. [PubMed: 9000048] [MGI Ref ID J:37854]
Hawes NL; Smith RS; Chang B; Davisson M; Heckenlively JR; John SW. 1999. Mouse fundus photography and angiography: a catalogue of normal and mutant phenotypes. Mol Vis 5:22. [PubMed: 10493779] [MGI Ref ID J:59481]
Keller SA; Jones JM; Boyle A; Barrow LL; Killen PD; Green DG; Kapousta NV; Hitchcock PF; Swank RT; Meisler MH. 1994. Kidney and retinal defects (Krd), a transgene-induced mutation with a deletion of mouse chromosome 19 that includes the Pax2 locus. Genomics 23(2):309-20. [PubMed: 7835879] [MGI Ref ID J:20807]
Nomura H; Turco AE; Pei Y; Kalaydjieva L; Schiavello T; Weremowicz S ; Ji W ; Morton CC ; Meisler M ; Reeders ST ; Zhou J. 1998. Identification of PKDL, a novel polycystic kidney disease 2-like gene whose murine homologue is deleted in mice with kidney and retinal defects. J Biol Chem 273(40):25967-73. [PubMed: 9748274] [MGI Ref ID J:50327]
Otteson DC; Shelden E; Jones JM; Kameoka J; Hitchcock PF. 1998. Pax2 expression and retinal morphogenesis in the normal and Krd mouse. Dev Biol 193(2):209-24. [PubMed: 9473325] [MGI Ref ID J:45929]
Urbanek P; Fetka I; Meisler MH; Busslinger M. 1997. Cooperation of Pax2 and Pax5 in midbrain and cerebellum development. Proc Natl Acad Sci U S A 94(11):5703-8. [PubMed: 9159136] [MGI Ref ID J:40910]
Dobkin C; Rabe A; Dumas R; El Idrissi A; Haubenstock H; Brown WT. 2000. Fmr1 knockout mouse has a distinctive strain-specific learning impairment. Neuroscience 100(2):423-9. [PubMed: 11008180] [MGI Ref ID J:119166]
Ivanco TL; Greenough WT. 2002. Altered mossy fiber distributions in adult Fmr1 (FVB) knockout mice. Hippocampus 12(1):47-54. [PubMed: 11918288] [MGI Ref ID J:113177]
Sakamoto K; McCluskey M; Wensel TG; Naggert JK; Nishina PM. 2009. New mouse models for recessive retinitis pigmentosa caused by mutations in the Pde6a gene. Hum Mol Genet 18(1):178-92. [PubMed: 18849587] [MGI Ref ID J:142108]
Zhao MG; Toyoda H; Ko SW; Ding HK; Wu LJ; Zhuo M. 2005. Deficits in trace fear memory and long-term potentiation in a mouse model for fragile X syndrome. J Neurosci 25(32):7385-92. [PubMed: 16093389] [MGI Ref ID J:100197]
Animal Health Reports
Room Number A1
Colony Maintenance
Mating System Heterozygote x Inbred (Female x Male) 11-MAR-11
| Pricing for USA, Canada and Mexico shipping destinations |
|
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $177.00 Female or Male Heterozygous for Krd
Price per Pair (US dollars $) Pair Genotype $286.30 Heterozygous for Krd x C3A.BLiA-Pde6b+/J (001912) Standard Supply
Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
| Pricing for International shipping destinations |
|
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $230.10 Female or Male Heterozygous for Krd
Price per Pair (US dollars $) Pair Genotype $372.20 Heterozygous for Krd x C3A.BLiA-Pde6b+/J (001912) Standard Supply
Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
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Research Strain. Availability determined by The Jackson Laboratory scientist holding the strain.
| Control | ||
|---|---|---|
| Wild-type from the colony | ||
| 001912 C3A.BLiA-Pde6b+/J | ||
| Considerations for Choosing Controls | ||
| Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
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