Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Chemically Induced Mutation; Mutant Stock; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation F+3

Description
Mice homozygous for the strigosus mutation are identifiable as early as six days of age by their elongated bodies and digits and a conical extension of the body at the base of the tail. Older mice are extremely thin and exhibit arachnodactyly, thoracic kyphosis, and, often, kinks in the tail. The strigosus mutation is milder than the longjohn alleles, which become humbacked at a younger age and have sacral kinks. Upon necropsy, homozygotes are found to lack normal body fat deposits. Skeletal analysis demonstrated delayed endochondral ossification, most obvious in the hind- and forepaws but affecting the entire skeleton. At embryonic day 10.5 all truncal vertebrae are present but enlarged.

Development
The strigosus mutation arose in 1989 in an outbred stock after mutagenesis with ethylnitrosourea and was bred onto 129/Sv. A belted mutation was also present in this predominantly 129 stock when it was imported into The Jackson Laboratory from the laboratory of Jean Louis Guenet at The Pasteur Institute. Mice were intercrossed and maintained segregating for the strigosus mutation and in 1996 embryos were cryopreserved from homozygous males bred with either homozygous or heterozygous females. The unspecified belted mutation remains in this stock.

Related Strains

Strains carrying other alleles of Npr3

003506	B6;C-Npr3lgj/J
008188	C57BL/6J-Npr3lgj-3J/J
003507	D2;B6-Npr3lgj-2J/J
008254	NOD/ShiLtJ-Npr3lgj-4J/J

View Strains carrying other alleles of Npr3     (4 strains)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Npr3^stri/Npr3^stri

        involves: 129/Sv * FVB/N

• skeleton phenotype
• abnormal axial skeleton morphology (MGI Ref ID J:57460)
  • easily distinguished from wild-type sibs as early as 6 days of age
• abnormal thoracic cage (MGI Ref ID J:57460)
  • partial thoracic supernumerary vertebrae with a small rib may occur
• abnormal vertebrae morphology (MGI Ref ID J:57460)
  • truncal vertebrae are enlarged
• abnormal caudal vertebrae morphology (MGI Ref ID J:57460)
• abnormal nucleus pulposus morphology (MGI Ref ID J:57460)
• absent nucleus pulposus (MGI Ref ID J:57460)
• abnormal sacral vertebrae morphology (MGI Ref ID J:57460)
  • transverse processes are sharper and more rostrally orientated than normal
• abnormal vertebrae development (MGI Ref ID J:57460)
• abnormal intervertebral disk development (MGI Ref ID J:57460)
  • discs are very thin
• abnormal vertebral epiphyseal plate morphology (MGI Ref ID J:57460)
  • abnormal proliferation at ventral and dorsal extremities, invading the vetebral bodies
• intervertebral calcification (MGI Ref ID J:57460)
• kyphosis (MGI Ref ID J:57460)
• abnormal skeleton development (MGI Ref ID J:57460)
• delayed endochondral bone ossification (MGI Ref ID J:57460)
  • evident in entire skeleton but most noticeable in fore- and hindpaws
  • observed in newborn mice
• abnormal skeleton extremities morphology (MGI Ref ID J:57460)
• abnormal carpal bone morphology (MGI Ref ID J:57460)
• abnormal metacarpal bone morphology (MGI Ref ID J:57460)
  • ossification centers not found in all metacarpals
  • delayed ossification most noticeable at metacarpal extremities
• abnormal phalanx morphology (MGI Ref ID J:57460)
  • delayed ossification is most noticeable at the extremities of phalanges
• limbs/digits/tail phenotype
• abnormal skeleton extremities morphology (MGI Ref ID J:57460)
• abnormal carpal bone morphology (MGI Ref ID J:57460)
• abnormal caudal vertebrae morphology (MGI Ref ID J:57460)
• abnormal metacarpal bone morphology (MGI Ref ID J:57460)
  • ossification centers not found in all metacarpals
  • delayed ossification most noticeable at metacarpal extremities
• abnormal phalanx morphology (MGI Ref ID J:57460)
  • delayed ossification is most noticeable at the extremities of phalanges
• adipose tissue phenotype
• abnormal abdominal fat pad morphology (MGI Ref ID J:57460)
• abnormal adipose tissue distribution (MGI Ref ID J:57460)

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Npr3^stri related

Developmental Biology Research
Skeletal Defects

Genes & Alleles

Gene & Allele Information

Allele Symbol		Npr3stri
Allele Name		strigosus
Allele Type		Chemically induced (ENU)
Common Name(s)		stri;
Strain of Origin		Outbred
Gene Symbol and Name		Npr3, natriuretic peptide receptor 3
Chromosome		15
Gene Common Name(s)		ANP-CR; ANPRC; B430320C24Rik; C81222; GUCY2B; NPR-C; NPRC; Nppc receptor; RIKEN cDNA B430320C24 gene; expressed sequence C81222; lgj; longjohn; stri; strigosus;
General Note		Genbank: AF131864
Molecular Note		A C-to-A transversion mutation at position 502 is predicted to lead to an asparagine to histidine substitution in a highly conserved region of the protein. [MGI Ref ID J:57460]

Genotyping

Genotyping Information

This strain will not have a genotyping protocol or one is not currently available.

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Jaubert J; Jaubert F; Martin N; Washburn LL; Lee BK; Eicher EM; Guenet JL. 1999. Three new allelic mouse mutations that cause skeletal overgrowth involve the natriuretic peptide receptor C gene (Npr3). Proc Natl Acad Sci U S A 96(18):10278-83. [PubMed: 10468599]  [MGI Ref ID J:57460]

Health & husbandry

Health & Colony Maintenance Information

Currently there no information available for this strain. This may be due to the supply level of this strain.

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery of Strains Needing Progeny Testing. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two untested males and two untested females (two pairs) will be recovered, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the overall recovery time to approximately 25 weeks. However, all pups recovered will be sent. Progeny testing is required to identify the genotype of mice of this strain, as a genotyping assay is not available. This type of testing involves breeding the recovered animals and assessing the phenotype of the offspring in order to identify animals carrying the mutation of interest. We can perform the progeny testing for you as a service or we can ship all recovered animals (at least two untested pairs) to you for progeny testing at your facility. If you perform the progeny testing, there is NO guarantee that a carrier will be identified. If we perform progeny testing as a service, additional breeding time will be required. In this case, when a male and female (one pair) are identified that carry the mutation, they and their offspring will be shipped. Delivery time for strains requiring progeny testing often exceeds 25 weeks and may take 12 months or more due to the difficulties in breeding some strains. The progeny testing cost is in addition to the recovery cost and is based on the number of boxes used and the time taken to produce the mice identified as carrying the mutation. Please note that identified pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Please contact Customer Service for more information on the cost of progeny testing for a strain: Tel: 1-800-422-6423 or 1-207-288-5845. Cryorecovery to establish a Dedicated Supply for greater quantities of mice One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

---

Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.