Strain Name:

B6.129S2-Lcktm1Mak/J

Stock Number:

002817

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Availability:

Cryopreserved - Ready for recovery

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Congenic; Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Additional information on Congenic nomenclature.
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S2 via D3 ES cell line
 
Donating InvestigatorDr. Tak Mak,   University Health Network/Un of Toronto

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Lcktm1Mak targeted mutation have a pronounced thymic atrophy. There is dramatic reduction in CD4+ CD8+ T cells. Mature single positive T cells are not detected. There are very few peripheral T cells. Also known as p56.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls

Related Strains

View Strains carrying other alleles of Lck     (12 strains)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Immunodeficiency 22; IMD22   (LCK)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Lcktm1Mak/Lcktm1Mak

        B6.129S2-Lcktm1Mak
  • immune system phenotype
  • abnormal T cell differentiation
    • intestinal intraepithelial lymphocytes (iIELs) is impaired   (MGI Ref ID J:111646)
    • however, extrathymic development of iIELs is normal in reconstitution experiments   (MGI Ref ID J:111646)
  • abnormal intraepithelial T cell morphology
    • in reconstitution experiments, intrathymic development of intestinal intraepithelial lymphocytes (iIELs) is impaired   (MGI Ref ID J:111646)
    • however, extrathymic development of iIELs is normal in reconstitution experiments   (MGI Ref ID J:111646)
    • abnormal intraepithelial T cell number
      • at 10 weeks, the number of intestinal intraepithelial lymphocytes (iIELs) is increased 1.5- to 2-fold compared to in wild-type mice   (MGI Ref ID J:111646)
      • the number of iIELs decrease over time unlike in wild-type mice that exhibit an increase in these cells   (MGI Ref ID J:111646)
      • decreased gamma-delta intraepithelial T cell number
        • at 10 weeks, mice exhibit decreased gamma-delta intestinal intraepithelial lymphocytes, which continue to decrease in number with age, compared with wild-type mice   (MGI Ref ID J:111646)
  • decreased cytotoxic T cell cytolysis
    • intestinal intraepithelial lymphocytes exhibit a slight reduction in cytolytic activities compared with wild-type cells   (MGI Ref ID J:111646)
  • hematopoietic system phenotype
  • abnormal T cell differentiation
    • intestinal intraepithelial lymphocytes (iIELs) is impaired   (MGI Ref ID J:111646)
    • however, extrathymic development of iIELs is normal in reconstitution experiments   (MGI Ref ID J:111646)
  • abnormal intraepithelial T cell morphology
    • in reconstitution experiments, intrathymic development of intestinal intraepithelial lymphocytes (iIELs) is impaired   (MGI Ref ID J:111646)
    • however, extrathymic development of iIELs is normal in reconstitution experiments   (MGI Ref ID J:111646)
    • abnormal intraepithelial T cell number
      • at 10 weeks, the number of intestinal intraepithelial lymphocytes (iIELs) is increased 1.5- to 2-fold compared to in wild-type mice   (MGI Ref ID J:111646)
      • the number of iIELs decrease over time unlike in wild-type mice that exhibit an increase in these cells   (MGI Ref ID J:111646)
      • decreased gamma-delta intraepithelial T cell number
        • at 10 weeks, mice exhibit decreased gamma-delta intestinal intraepithelial lymphocytes, which continue to decrease in number with age, compared with wild-type mice   (MGI Ref ID J:111646)
  • decreased cytotoxic T cell cytolysis
    • intestinal intraepithelial lymphocytes exhibit a slight reduction in cytolytic activities compared with wild-type cells   (MGI Ref ID J:111646)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Lcktm1Mak/Lcktm1Mak

        involves: 129S2/SvPas
  • immune system phenotype
  • abnormal lymphocyte morphology
    • although the overall numbers of cells in the lymph nodes and spleens are normal the B/T cells ratio is inverted with 90 to 95% B cells and 5 to 10% T cells   (MGI Ref ID J:1119)
    • abnormal T cell morphology
      • double-positive thymocytes have a slightly lower expression of alpha-beta TCR   (MGI Ref ID J:110847)
      • abnormal alpha-beta intraepithelial T cell morphology
        • alpha-beta IEL are absent in these mice   (MGI Ref ID J:112977)
        • a CD3- IEL population believed to represent a precursor population is present in small intestine epithelium   (MGI Ref ID J:112977)
      • abnormal double-negative T cell morphology
        • there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes   (MGI Ref ID J:110847)
      • abnormal single-positive T cell number
        • very few single-positive thymocytes are detected in these mice and even fewer express mature levels of TCR, CD5 and CD69   (MGI Ref ID J:37062)
      • abnormal thymocyte activation
        • double positive thymocytes do not efficiently enter into the cell cycle   (MGI Ref ID J:110847)
      • decreased T cell number
        • the number of peripheral T cells is reduced   (MGI Ref ID J:1119)
        • alpha-beta T cell numbers are reduced about 10-20 fold compared to controls   (MGI Ref ID J:37062)
        • decreased CD4-positive, alpha beta T cell number
          • CD4 T cell numbers are reduced in the periphery   (MGI Ref ID J:37940)
        • decreased CD8-positive, alpha-beta T cell number
          • CD8 T cell numbers are reduced in the periphery   (MGI Ref ID J:37940)
        • decreased double-positive T cell number
          • 0.3x107 to 1.6x107 compared to 1.8x108 cells in wild-type mice   (MGI Ref ID J:1119)
          • only 12% of thymocytes are double-positive compared to 97% in controls   (MGI Ref ID J:110847)
        • decreased gamma-delta T cell number
          • gamma-delta T cell numbers are reduced about 2-fold compared to controls   (MGI Ref ID J:37062)
          • decreased gamma-delta intraepithelial T cell number
            • dendritic epithelial T cell numbers are reduced in the skin   (MGI Ref ID J:37940)
            • gamma-delta IEL are absent in these mice   (MGI Ref ID J:112977)
            • a CD3- IEL population believed to represent a precursor population is present in small intestine epithelium   (MGI Ref ID J:112977)
        • decreased thymocyte number
          • 6x106 to 2x107 cells compared to 2x108 cells in a wild-type thymus   (MGI Ref ID J:1119)
  • decreased IgG1 level   (MGI Ref ID J:1119)
  • increased IgG2a level
    • marginal   (MGI Ref ID J:1119)
  • increased IgG3 level
    • marginal   (MGI Ref ID J:1119)
  • increased IgM level   (MGI Ref ID J:1119)
  • thymus atrophy
    • the thymus is sparsely populated with lymphoid cells and has a very thin medulla   (MGI Ref ID J:1119)
  • thymus hypoplasia
    • thymus cellularity is 10-20% of normal   (MGI Ref ID J:37062)
    • thymic cellularity is 10-fold less than controls   (MGI Ref ID J:37940)
  • hematopoietic system phenotype
  • abnormal lymphocyte morphology
    • although the overall numbers of cells in the lymph nodes and spleens are normal the B/T cells ratio is inverted with 90 to 95% B cells and 5 to 10% T cells   (MGI Ref ID J:1119)
    • abnormal T cell morphology
      • double-positive thymocytes have a slightly lower expression of alpha-beta TCR   (MGI Ref ID J:110847)
      • abnormal alpha-beta intraepithelial T cell morphology
        • alpha-beta IEL are absent in these mice   (MGI Ref ID J:112977)
        • a CD3- IEL population believed to represent a precursor population is present in small intestine epithelium   (MGI Ref ID J:112977)
      • abnormal double-negative T cell morphology
        • there is a partial block in development in the DN3 stage that leads to a higher percentage of these thymocytes   (MGI Ref ID J:110847)
      • abnormal single-positive T cell number
        • very few single-positive thymocytes are detected in these mice and even fewer express mature levels of TCR, CD5 and CD69   (MGI Ref ID J:37062)
      • abnormal thymocyte activation
        • double positive thymocytes do not efficiently enter into the cell cycle   (MGI Ref ID J:110847)
      • decreased T cell number
        • the number of peripheral T cells is reduced   (MGI Ref ID J:1119)
        • alpha-beta T cell numbers are reduced about 10-20 fold compared to controls   (MGI Ref ID J:37062)
        • decreased CD4-positive, alpha beta T cell number
          • CD4 T cell numbers are reduced in the periphery   (MGI Ref ID J:37940)
        • decreased CD8-positive, alpha-beta T cell number
          • CD8 T cell numbers are reduced in the periphery   (MGI Ref ID J:37940)
        • decreased double-positive T cell number
          • 0.3x107 to 1.6x107 compared to 1.8x108 cells in wild-type mice   (MGI Ref ID J:1119)
          • only 12% of thymocytes are double-positive compared to 97% in controls   (MGI Ref ID J:110847)
        • decreased gamma-delta T cell number
          • gamma-delta T cell numbers are reduced about 2-fold compared to controls   (MGI Ref ID J:37062)
          • decreased gamma-delta intraepithelial T cell number
            • dendritic epithelial T cell numbers are reduced in the skin   (MGI Ref ID J:37940)
            • gamma-delta IEL are absent in these mice   (MGI Ref ID J:112977)
            • a CD3- IEL population believed to represent a precursor population is present in small intestine epithelium   (MGI Ref ID J:112977)
        • decreased thymocyte number
          • 6x106 to 2x107 cells compared to 2x108 cells in a wild-type thymus   (MGI Ref ID J:1119)
  • decreased IgG1 level   (MGI Ref ID J:1119)
  • increased IgG2a level
    • marginal   (MGI Ref ID J:1119)
  • increased IgG3 level
    • marginal   (MGI Ref ID J:1119)
  • increased IgM level   (MGI Ref ID J:1119)
  • thymus atrophy
    • the thymus is sparsely populated with lymphoid cells and has a very thin medulla   (MGI Ref ID J:1119)
  • thymus hypoplasia
    • thymus cellularity is 10-20% of normal   (MGI Ref ID J:37062)
    • thymic cellularity is 10-fold less than controls   (MGI Ref ID J:37940)
  • endocrine/exocrine gland phenotype
  • abnormal thymocyte activation
    • double positive thymocytes do not efficiently enter into the cell cycle   (MGI Ref ID J:110847)
  • decreased thymocyte number
    • 6x106 to 2x107 cells compared to 2x108 cells in a wild-type thymus   (MGI Ref ID J:1119)
  • thymus atrophy
    • the thymus is sparsely populated with lymphoid cells and has a very thin medulla   (MGI Ref ID J:1119)
  • thymus hypoplasia
    • thymus cellularity is 10-20% of normal   (MGI Ref ID J:37062)
    • thymic cellularity is 10-fold less than controls   (MGI Ref ID J:37940)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Lcktm1Mak related

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Lcktm1Mak
Allele Name targeted mutation 1, Tak Mak
Allele Type Targeted (Null/Knockout)
Common Name(s) Lck-deficient; lck-; lcknull; p56lck;
Mutation Made ByDr. Tak Mak,   University Health Network/Un of Toronto
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Lck, lymphocyte protein tyrosine kinase
Chromosome 4
Gene Common Name(s) Hck-3; IMD22; LSK; Lck1; Lcktkr; YT16; hemopoietic cell kinase 3; p56lck; p56lck; pp58lck;
Molecular Note A neomycin resistance cassette was inserted into exon 12 of the gene. Western blots of lysates from thymus or lymph nodes showed no detectable protein from the targeted gene. [MGI Ref ID J:1119]

Genotyping

Genotyping Information

Genotyping Protocols

Lcktm1Mak, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Molina TJ; Kishihara K; Siderovski DP; van Ewijk W; Narendran A; Timms E; Wakeham A; Paige CJ; Hartmann KU; Veillette A; Davidson D; Mak TW. 1992. Profound block in thymocyte development in mice lacking p56lck [see comments] Nature 357(6374):161-4. [PubMed: 1579166]  [MGI Ref ID J:1119]

Additional References

Dal Porto JM; Burke K; Cambier JC. 2004. Regulation of BCR signal transduction in B-1 cells requires the expression of the Src family kinase Lck. Immunity 21(3):443-53. [PubMed: 15357954]  [MGI Ref ID J:93928]

Lcktm1Mak related

Anderson SJ; Levin SD; Perlmutter RM. 1993. Protein tyrosine kinase p56lck controls allelic exclusion of T-cell receptor beta-chain genes. Nature 365(6446):552-4. [PubMed: 8413611]  [MGI Ref ID J:76888]

Assarsson E; Kambayashi T; Sandberg JK; Hong S; Taniguchi M; Van Kaer L; Ljunggren HG; Chambers BJ. 2000. CD8+ T cells rapidly acquire NK1.1 and NK cell-associated molecules upon stimulation in vitro and in vivo. J Immunol 165(7):3673-9. [PubMed: 11034371]  [MGI Ref ID J:118029]

Baur N; Nerz G; Nil A; Eichmann K. 2001. Expression and selection of productively rearranged TCRbeta VDJ genes are sequentially regulated by CD3 signaling in the development of NK1.1(+) alphabeta T cells. Int Immunol 13(8):1031-42. [PubMed: 11470773]  [MGI Ref ID J:71151]

Biro J; Wurch A; Potocnik AJ; Falk I; Mossmann H; Eichmann K. 1999. Regulation of T cell receptor (TCR) beta gene expression by CD3 complex signaling in immature thymocytes: implications for TCRbeta allelic exclusion. Proc Natl Acad Sci U S A 96(7):3882-7. [PubMed: 10097132]  [MGI Ref ID J:123031]

Caserta S; Kleczkowska J; Mondino A; Zamoyska R. 2010. Reduced functional avidity promotes central and effector memory CD4 T cell responses to tumor-associated antigens. J Immunol 185(11):6545-54. [PubMed: 21048115]  [MGI Ref ID J:166130]

Chu DH; van Oers NS; Malissen M; Harris J; Elder M; Weiss A. 1999. Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression. J Immunol 163(5):2610-20. [PubMed: 10453000]  [MGI Ref ID J:57092]

Dong Z; Davidson D; Perez-Quintero LA; Kurosaki T; Swat W; Veillette A. 2012. The adaptor SAP controls NK cell activation by regulating the enzymes Vav-1 and SHIP-1 and by enhancing conjugates with target cells. Immunity 36(6):974-85. [PubMed: 22683124]  [MGI Ref ID J:187430]

Engel I; Murre C. 2004. E2A proteins enforce a proliferation checkpoint in developing thymocytes. EMBO J 23(1):202-11. [PubMed: 14685278]  [MGI Ref ID J:115665]

Engelmann S; Togni M; Thielitz A; Reichardt P; Kliche S; Reinhold D; Schraven B; Reinhold A. 2013. T cell-independent modulation of experimental autoimmune encephalomyelitis in ADAP-deficient mice. J Immunol 191(10):4950-9. [PubMed: 24101551]  [MGI Ref ID J:206334]

Fujise S; Matsuzaki G; Kishihara K; Kadena T; Molina T; Nomoto K. 1996. The role of p56lck in the development of gamma delta T cells and their function during an infection by Listeria monocytogenes. J Immunol 157(1):247-54. [PubMed: 8683122]  [MGI Ref ID J:110636]

Gadue P; Morton N; Stein PL. 1999. The Src family tyrosine kinase Fyn regulates natural killer T cell development. J Exp Med 190(8):1189-96. [PubMed: 10523617]  [MGI Ref ID J:110943]

Gadue P; Yin L; Jain S; Stein PL. 2004. Restoration of NK T cell development in fyn-mutant mice by a TCR reveals a requirement for Fyn during early NK T cell ontogeny. J Immunol 172(10):6093-100. [PubMed: 15128794]  [MGI Ref ID J:89871]

Garrett-Engele CM; Tasch MA; Hwang HC; Fero ML; Perlmutter RM; Clurman BE; Roberts JM. 2007. A mechanism misregulating p27 in tumors discovered in a functional genomic screen. PLoS Genet 3(12):e219. [PubMed: 18069898]  [MGI Ref ID J:133427]

Groves T; Smiley P; Cooke MP; Forbush K; Perlmutter RM; Guidos CJ. 1996. Fyn can partially substitute for Lck in T lymphocyte development. Immunity 5(5):417-28. [PubMed: 8934569]  [MGI Ref ID J:37062]

Guimond MJ; Luross JA; Wang B; Terhorst C; Danial S; Croy BA. 1997. Absence of natural killer cells during murine pregnancy is associated with reproductive compromise in TgE26 mice. Biol Reprod 56(1):169-79. [PubMed: 9002646]  [MGI Ref ID J:134589]

Huang J; Lo PF; Zal T; Gascoigne NR; Smith BA; Levin SD; Grey HM. 2002. CD28 plays a critical role in the segregation of PKC theta within the immunologic synapse. Proc Natl Acad Sci U S A 99(14):9369-73. [PubMed: 12077322]  [MGI Ref ID J:126523]

Kawai K; Kishihara K; Molina TJ; Wallace VA; Mak TW; Ohashi PS. 1995. Impaired development of V gamma 3 dendritic epidermal T cells in p56lck protein tyrosine kinase-deficient and CD45 protein tyrosine phosphatase-deficient mice. J Exp Med 181(1):345-9. [PubMed: 7807014]  [MGI Ref ID J:22470]

Kemp KL; Levin SD; Bryce PJ; Stein PL. 2010. Lck mediates Th2 differentiation through effects on T-bet and GATA-3. J Immunol 184(8):4178-84. [PubMed: 20237292]  [MGI Ref ID J:159870]

Kemp KL; Levin SD; Stein PL. 2010. Lck regulates IL-10 expression in memory-like Th1 cells. Eur J Immunol 40(11):3210-9. [PubMed: 21061443]  [MGI Ref ID J:166659]

Krotkova A; Smith E; Nerz G; Falk I; Eichmann K. 2004. Delayed and restricted expression limits putative instructional opportunities of Vgamma1.1/Vgamma2 gammadelta TCR in alphabeta/gammadelta lineage choice in the thymus. J Immunol 173(1):25-32. [PubMed: 15210755]  [MGI Ref ID J:90818]

Laird RM; Hayes SM. 2010. Roles of the Src tyrosine kinases Lck and Fyn in regulating gammadeltaTCR signal strength. PLoS One 5(1):e8899. [PubMed: 20126650]  [MGI Ref ID J:157616]

Laird RM; Laky K; Hayes SM. 2010. Unexpected role for the B cell-specific Src family kinase B lymphoid kinase in the development of IL-17-producing gammadelta T cells. J Immunol 185(11):6518-27. [PubMed: 20974990]  [MGI Ref ID J:167365]

Lee WH; Ramos T; Krymskaya L; Liu CP. 2003. Development of T cells expressing an altered TCR complex. Eur J Immunol 33(10):2696-705. [PubMed: 14515253]  [MGI Ref ID J:115654]

Lesage S; Steff AM; Philippoussis F; Page M; Trop S; Mateo V ; Hugo P. 1997. CD4+ CD8+ thymocytes are preferentially induced to die following CD45 cross-linking, through a novel apoptotic pathway. J Immunol 159(10):4762-71. [PubMed: 9366400]  [MGI Ref ID J:44075]

Levelt CN; Mombaerts P; Wang B; Kohler H; Tonegawa S; Eichmann K; Terhorst C. 1995. Regulation of thymocyte development through CD3: functional dissociation between p56lck and CD3 sigma in early thymic selection. Immunity 3(2):215-22. [PubMed: 7648394]  [MGI Ref ID J:28295]

Liao XC; Littman DR; Weiss A. 1997. Itk and Fyn make independent contributions to T cell activation. J Exp Med 186(12):2069-73. [PubMed: 9396778]  [MGI Ref ID J:44879]

Lin K; Longo NS; Wang X; Hewitt JA; Abraham KM. 2000. Lck domains differentially contribute to pre-T cell receptor (TCR)- and TCR-alpha/beta-regulated developmental transitions. J Exp Med 191(4):703-16. [PubMed: 10684862]  [MGI Ref ID J:124696]

Liu P; Aitken K; Kong YY; Opavsky MA; Martino T; Dawood F; Wen WH; Kozieradzki I; Bachmaier K; Straus D; Mak TW; Penninger JM. 2000. The tyrosine kinase p56lck is essential in coxsackievirus B3-mediated heart disease. Nat Med 6(4):429-34. [PubMed: 10742150]  [MGI Ref ID J:119597]

Ljutic B; Carlyle JR; Filipp D; Nakagawa R; Julius M; Zuniga-Pflucker JC. 2005. Functional requirements for signaling through the stimulatory and inhibitory mouse NKR-P1 (CD161) NK cell receptors. J Immunol 174(8):4789-96. [PubMed: 15814704]  [MGI Ref ID J:98147]

Lovatt M; Filby A; Parravicini V; Werlen G; Palmer E; Zamoyska R. 2006. Lck Regulates the Threshold of Activation in Primary T Cells, While both Lck and Fyn Contribute to the Magnitude of the Extracellular Signal-Related Kinase Response. Mol Cell Biol 26(22):8655-65. [PubMed: 16966372]  [MGI Ref ID J:114668]

Lowin-Kropf B; Kunz B; Schneider P; Held W. 2002. A role for the src family kinase Fyn in NK cell activation and the formation of the repertoire of Ly49 receptors. Eur J Immunol 32(3):773-82. [PubMed: 11870621]  [MGI Ref ID J:108602]

Mason LH; Willette-Brown J; Taylor LS; McVicar DW. 2006. Regulation of Ly49D/DAP12 signal transduction by Src-family kinases and CD45. J Immunol 176(11):6615-23. [PubMed: 16709819]  [MGI Ref ID J:131771]

Molina TJ; Bachmann MF; Kundig TM; Zinkernagel RM; Mak TW. 1993. Peripheral T cells in mice lacking p56lck do not express significant antiviral effector functions. J Immunol 151(2):699-706. [PubMed: 8393038]  [MGI Ref ID J:43746]

Molina TJ; Perrot JY; Penninger J; Ramos A; Audouin J; Briand P; Mak TW; Diebold J. 1998. Differential requirement for p56lck in fetal and adult thymopoiesis. J Immunol 160(8):3828-34. [PubMed: 9558087]  [MGI Ref ID J:47242]

Mombaerts P; Anderson SJ; Perlmutter RM; Mak TW; Tonegawa S. 1994. An activated lck transgene promotes thymocyte development in RAG-1 mutant mice. Immunity 1(4):261-7. [PubMed: 7889413]  [MGI Ref ID J:21066]

Oliveira-dos-Santos AJ; Penninger JM; Rieker-Geley T; Matsumoto G; Mak TM; Wick G. 1998. Thymic heterotypic cellular complexes in gene-targeted mice with defined blocks in T cell development and adhesion molecule expression. Eur J Immunol 28(9):2882-92. [PubMed: 9754575]  [MGI Ref ID J:49877]

Omri B; Blancher C; Neron B; Marty MC; Rutin J; Molina TJ; Pessac B; Crisanti P. 1998. Retinal dysplasia in mice lacking p56lck. Oncogene 16(18):2351-6. [PubMed: 9620552]  [MGI Ref ID J:47694]

Page ST; van Oers NS; Perlmutter RM; Weiss A; Pullen AM. 1997. Differential contribution of Lck and Fyn protein tyrosine kinases to intraepithelial lymphocyte development. Eur J Immunol 27(2):554-62. [PubMed: 9045930]  [MGI Ref ID J:112977]

Penninger J; Kishihara K; Molina T; Wallace VA; Timms E; Hedrick SM ; Mak TW. 1993. Requirement for tyrosine kinase p56lck for thymic development of transgenic gamma delta T cells. Science 260(5106):358-61. [PubMed: 8469988]  [MGI Ref ID J:43745]

Pineau T; Fernandez-Salguero P; Lee SS; McPhail T; Ward JM; Gonzalez FJ. 1995. Neonatal lethality associated with respiratory distress in mice lacking cytochrome P450 1A2. Proc Natl Acad Sci U S A 92(11):5134-8. [PubMed: 7761462]  [MGI Ref ID J:25724]

Ping P; Song C; Zhang J; Guo Y; Cao X; Li RC; Wu W; Vondriska TM; Pass JM; Tang XL; Pierce WM; Bolli R. 2002. Formation of protein kinase C(epsilon)-Lck signaling modules confers cardioprotection. J Clin Invest 109(4):499-507. [PubMed: 11854322]  [MGI Ref ID J:74698]

Salmond RJ; Filby A; Pirinen N; Magee AI; Zamoyska R. 2011. Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas. Blood 117(1):108-17. [PubMed: 20876849]  [MGI Ref ID J:168430]

Sato T; Kishihara K; Mak TW; Habu S. 1998. Beta-selection of immature thymocytes is less dependent on CD45 tyrosinephosphatase. Immunol Lett 64(2-3):133-8. [PubMed: 9870664]  [MGI Ref ID J:52164]

Seddon B; Legname G; Tomlinson P; Zamoyska R. 2000. Long-term survival but impaired homeostatic proliferation of Naive T cells in the absence of p56(lck) Science 290(5489):127-31. [PubMed: 11021796]  [MGI Ref ID J:65101]

Seddon B; Tomlinson P; Zamoyska R. 2003. Interleukin 7 and T cell receptor signals regulate homeostasis of CD4 memory cells. Nat Immunol 4(7):680-6. [PubMed: 12808452]  [MGI Ref ID J:129178]

Seddon B; Zamoyska R. 2002. TCR signals mediated by Src family kinases are essential for the survival of naive T cells. J Immunol 169(6):2997-3005. [PubMed: 12218114]  [MGI Ref ID J:133734]

Sharif-Askari E; Gaucher D; Halwani R; Ma J; Jao K; Abdallah A; Haddad EK; Sekaly RP. 2007. p56Lck tyrosine kinase enhances the assembly of death-inducing signaling complex during Fas-mediated apoptosis. J Biol Chem 282(49):36048-56. [PubMed: 17932036]  [MGI Ref ID J:129029]

Sicinska E; Aifantis I; Le Cam L; Swat W; Borowski C; Yu Q; Ferrando AA; Levin SD; Geng Y; von Boehmer H; Sicinski P. 2003. Requirement for cyclin D3 in lymphocyte development and T cell leukemias. Cancer Cell 4(6):451-61. [PubMed: 14706337]  [MGI Ref ID J:88120]

Tewari K; Walent J; Svaren J; Zamoyska R; Suresh M. 2006. Differential requirement for Lck during primary and memory CD8+ T cell responses. Proc Natl Acad Sci U S A 103(44):16388-93. [PubMed: 17060632]  [MGI Ref ID J:115596]

Trobridge PA; Forbush KA; Levin SD. 2001. Positive and negative selection of thymocytes depends on Lck interaction with the CD4 and CD8 coreceptors. J Immunol 166(2):809-18. [PubMed: 11145654]  [MGI Ref ID J:127034]

Trobridge PA; Levin SD. 2001. Lck plays a critical role in Ca(2+) mobilization and CD28 costimulation in mature primary T cells. Eur J Immunol 31(12):3567-79. [PubMed: 11745376]  [MGI Ref ID J:128169]

Ulivieri C; Valensin S; Majolini MB; Matthews RJ; Baldari CT. 2003. Normal B-1 cell development but defective BCR signaling in Lck-/- mice. Eur J Immunol 33(2):441-5. [PubMed: 12645942]  [MGI Ref ID J:115705]

Van Laethem F; Tikhonova AN; Pobezinsky LA; Tai X; Kimura MY; Le Saout C; Guinter TI; Adams A; Sharrow SO; Bernhardt G; Feigenbaum L; Singer A. 2013. Lck availability during thymic selection determines the recognition specificity of the T cell repertoire. Cell 154(6):1326-41. [PubMed: 24034254]  [MGI Ref ID J:204615]

Visan I; Yuan JS; Liu Y; Stanley P; Guidos CJ. 2010. Lunatic fringe enhances competition for delta-like Notch ligands but does not overcome defective pre-TCR signaling during thymocyte beta-selection in vivo. J Immunol 185(8):4609-17. [PubMed: 20844195]  [MGI Ref ID J:164883]

Wallace VA; Kawai K; Levelt CN; Kishihara K; Molina T; Timms E; Pircher H; Penninger J; Ohashi PS; Eichmann K; Mak TW. 1995. T lymphocyte development in p56lck deficient mice: allelic exclusion of the TcR beta locus is incomplete but thymocyte development is not restored by TcR beta or TcR alpha beta transgenes. Eur J Immunol 25(5):1312-8. [PubMed: 7774634]  [MGI Ref ID J:25742]

Wallace VA; Penninger J; Mak TW. 1994. T-Cell development in CD4, CD8, and p56(lck) Gene-Targeted mice.. In: Transgenesis and Targeted Mutagenesis in Immunology. Academic Press, Inc..  [MGI Ref ID J:21662]

Wen T; Zhang L; Kung SK; Molina TJ; Miller RG; Mak TW. 1995. Allo-skin graft rejection, tumor rejection and natural killer activity in mice lacking p56lck. Eur J Immunol 25(11):3155-9. [PubMed: 7489757]  [MGI Ref ID J:29801]

Wu G; Danska JS; Guidos CJ. 1996. Lck dependence of signaling pathways activated by gamma-irradiation and CD3 epsilon engagement in RAG-1(-/-)-immature thymocytes. Int Immunol 8(7):1159-64. [PubMed: 8757961]  [MGI Ref ID J:37930]

Wurch A; Biro J; Falk I; Mossmann H; Eichmann K. 1999. Reduced generation but efficient TCR beta-chain selection of CD4+8+ double-positive thymocytes in mice with compromised CD3 complex signaling. J Immunol 162(5):2741-7. [PubMed: 10072519]  [MGI Ref ID J:110847]

Wurch A; Biro J; Potocnik AJ; Falk I; Mossmann H; Eichmann K. 1998. Requirement of CD3 complex-associated signaling functions for expression of rearranged T cell receptor beta VDJ genes in early thymic development. J Exp Med 188(9):1669-78. [PubMed: 9802979]  [MGI Ref ID J:112046]

Yada S; Kishihara K; Kong YY; Nomoto K. 1998. Differential requirements of CD45 protein tyrosine phosphatase for cytolytic activities and intrathymic and extrathymic development of intestinal intraepithelial lymphocytes. J Immunol 161(5):2208-16. [PubMed: 9725213]  [MGI Ref ID J:111646]

Yamada H; Kong YY; Kishihara K; Mak TW; Nomoto K. 1997. p56lck is not essential for the T-cell response to allo-MHC antigens. Immunology 92(1):33-8. [PubMed: 9370921]  [MGI Ref ID J:42630]

de la Roche M; Ritter AT; Angus KL; Dinsmore C; Earnshaw CH; Reiter JF; Griffiths GM. 2013. Hedgehog signaling controls T cell killing at the immunological synapse. Science 342(6163):1247-50. [PubMed: 24311692]  [MGI Ref ID J:205261]

van Oers NS; Lowin-Kropf B; Finlay D; Connolly K; Weiss A. 1996. alpha beta T cell development is abolished in mice lacking both Lck and Fyn protein tyrosine kinases. Immunity 5(5):429-36. [PubMed: 8934570]  [MGI Ref ID J:37940]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryHomozygotes are fertile. Expected coat color from breeding: black

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   000664 C57BL/6J
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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