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Strain Name:

B6.129S2-Lcktm1Mak/J

Stock Number:

002817

Availability:

Repository-Cryopreserved

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Additional Licensing and Use Restrictions

Genes & Alleles   Lck;   Lcktm1Mak;

Product Information

Strain Details

Type JAX® GEMM® Strain - Congenic
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Mutant Strain
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Background Strain C57BL/6
Donor Strain 129S2 via D3 ES cell line
Donating Investigator Tak Mak,   University Health Network/Un of Toronto

Appearance
black
Related Genotype: a/a

Strain Description
Mice homozygous for the Lcktm1Mak targeted mutation have a pronounced thymic atrophy. There is dramatic reduction in CD4+ CD8+ T cells. Mature single positive T cells are not detected. There are very few peripheral T cells. Also known as p56.

Mammalian Phenotype Terms assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Lcktm1Mak/Lcktm1Mak

        involves: 129S2/SvPas
  • immune system phenotype
  • abnormal lymphocyte morphology (MGI Ref ID J:1119)
    • although the overall numbers of cells in the lymph nodes and spleens are normal the B/T cells ratio is inverted with 90 to 95% B cells and 5 to 10% T cells
    • decreased T cell number (MGI Ref ID J:1119)
      • the number of peripheral T cells is reduced
      • absent single-positive T cells (MGI Ref ID J:1119)
      • decreased double-positive T cell number (MGI Ref ID J:1119)
        • 0.3x107 to 1.6x107 compared to 1.8x108 cells in wild type mice
      • decreased thymocyte number (MGI Ref ID J:1119)
        • 6x106 to 2x107 cells compared to 2x108 cells in a wild type thymus
  • decreased IgG1 level (MGI Ref ID J:1119)
  • increased IgG2a level (MGI Ref ID J:1119)
    • marginal
  • increased IgG3 level (MGI Ref ID J:1119)
    • marginal
  • increased IgM level (MGI Ref ID J:1119)
  • thymus atrophy (MGI Ref ID J:1119)
    • the thymus is sparsely populated with lymphoid cells and has a very thin medulla
  • hematopoietic system phenotype
  • abnormal lymphocyte morphology (MGI Ref ID J:1119)
    • although the overall numbers of cells in the lymph nodes and spleens are normal the B/T cells ratio is inverted with 90 to 95% B cells and 5 to 10% T cells
    • decreased T cell number (MGI Ref ID J:1119)
      • the number of peripheral T cells is reduced
      • absent single-positive T cells (MGI Ref ID J:1119)
      • decreased double-positive T cell number (MGI Ref ID J:1119)
        • 0.3x107 to 1.6x107 compared to 1.8x108 cells in wild type mice
      • decreased thymocyte number (MGI Ref ID J:1119)
        • 6x106 to 2x107 cells compared to 2x108 cells in a wild type thymus
  • thymus atrophy (MGI Ref ID J:1119)
    • the thymus is sparsely populated with lymphoid cells and has a very thin medulla

Gene & Allele Details

Allele Symbol Lcktm1Mak
Allele Name targeted mutation 1, Tak Mak
Common Name(s) Lck-deficient; lck-; lcknull; p56lck;
Mutation Made By Tak Mak,   University Health Network/Un of Toronto
Strain of Origin129S2/SvPas
ES Cell Line NameD3
ES Cell Line Strain129S2/SvPas
Gene Symbol and Name Lck, lymphocyte protein tyrosine kinase
Chromosome 4
Gene Common Name(s) Hck-3; Lck1; Lcktkr; YT16; hemopoietic cell kinase 3; p56lck; p56lck; pp58lck;
Molecular Note A neomycin resistance cassette was inserted into exon 12 of the gene. Western blots of lysates from thymus or lymph nodes showed no detectable protein from the targeted gene. [MGI Ref ID J:1119]

Control Information

  Allele   Control
 Lcktm1Mak  000664 C57BL/6J
 
  Considerations for Choosing Controls

Genotyping Protocols

Lcktm1Mak

Colony Maintenance

Breeding & HusbandryHomozygotes are fertile. Expected coat color from breeding: black

Related Strains

Strains carrying other alleles of Lck
003802   B6.Cg-Tg(Lck-cre)548Jxm/J
002427   C3H/He-Tg(LCKprBCL2)36Sjk/J
003332   C57BL/6-Tg(LckNotch1)9Erob/J
002230   C57BL/6J-Tg(LckIl4)1315Dbl/J
005732   NOD.Cg-Tg(Lck-cre)548Jxm/AchJ
View Strains carrying other alleles of Lck     (5 strains)

Additional Web Information

Congenic Nomenclature

Research Applications

This mouse can be used to support research in many areas including:

Lcktm1Mak related

Immunology and Inflammation Research
Immunodeficiency

References

Selected Reference(s)

Molina TJ; Kishihara K; Siderovski DP; van Ewijk W; Narendran A; Timms E; Wakeham A; Paige CJ; Hartmann KU; Veillette A; Davidson D; Mak TW. 1992. Profound block in thymocyte development in mice lacking p56lck [see comments] Nature 357(6374):161-4. [PubMed: 1579166]  [MGI Ref ID J:1119]

Additional References

Price and Supply Information

Strain Name: B6.129S2-Lcktm1Mak/J
Stock Number: 002817

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below for Licensing and Use Restrictions  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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For additional Licensing and Use Restrictions view the link(s) below:
- Strain(s) not available to companies or for-profit entities.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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