Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse   Donating Investigator Robert Hammer,   U. of Texas, SW Medical Center at Dallas

Appearance
multiple coat colors
Related Genotype: a, A, Oca2^p, Tyr^c and Pde6b^rd1

Control Information

	Control
	Noncarrier
Considerations for Choosing Controls

Related Strains

Strains carrying other alleles of SREBF1

003393

B6;SJL-Tg(aP2-SREBF1c)9884Reh/J

View Strains carrying other alleles of SREBF1     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

Tg(SREBP1a)7343Reh/0

        involves: C57BL/6J * SJL/J

• homeostasis/metabolism phenotype
• *normal* homeostasis/metabolism phenotype (MGI Ref ID J:35770)
  • despite increases in liver lipid synthesis and content after being fed a low carbohydrate/high protein diet for 2 weeks, mice exhibit normal plasma glucose, creatinine, alkaline phophatase, bilirubin, and blood urea nitrogen levels
• abnormal lipid homeostasis (MGI Ref ID J:35770)
  • lipid biosynthesis is increased compared to in wild-type mice
• decreased circulating triglyceride level (MGI Ref ID J:35770)
  • modest
• increased cholesterol level (MGI Ref ID J:35770)
  • after being fed a low carbohydrate/high protein diet for 2 weeks, liver cholesterol levels are increased 4.7-fold compared to in wild-type mice
  • however, plasma cholesterol levels are normal
• increased triglyceride level (MGI Ref ID J:35770)
  • after being fed a low carbohydrate/high protein diet for 2 weeks, liver triglyceride levels are increased 21-fold compared to in wild-type mice
• hyperalbuminemia (MGI Ref ID J:35770)
• increased circulating aspartate transaminase level (MGI Ref ID J:35770)
• increased circulating total protein level (MGI Ref ID J:35770)
• liver/biliary system phenotype
• *normal* liver/biliary system phenotype (MGI Ref ID J:35770)
  • despite accumulation of fat in the liver, mice do not exhibit generalized inflammation in the liver or liver necrosis
• abnormal hepatocyte morphology (MGI Ref ID J:35770)
  • at 16 weeks of age mice exhibit distended hepatocytes due to fat droplet accumulation
• enlarged liver (MGI Ref ID J:35770)
  • after birth, mice exhibit abdominal swelling due to an enlarged liver than continues to enlarge after weaning when mice are placed on a low carbohydrate/high protein diet
  • liver enlargement occurs due to increased lipid accumulation
• increased liver weight (MGI Ref ID J:35770)
  • after being fed a low carbohydrate/high protein diet for 2 weeks, liver weight is increased 4-fold compared to similarly treated wild-type mice
• pale liver (MGI Ref ID J:35770)
• adipose tissue phenotype
• abnormal fat pad morphology (MGI Ref ID J:35770)
  • mice exhibit a decrease in subcutaneous, omental, perirenal and epididymal fat compared to in wild-type mice
• abnormal epididymal fat pad morphology (MGI Ref ID J:35770)
  • after being fed a low carbohydrate/high protein diet for 2 weeks, epididymal fat pad weight is decreased by more than 90% compared to similarly treated wild-type mice

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Tg(SREBP1a)7343Reh/0

        B6.Cg-Tg(SREBP1a)7343Reh

• homeostasis/metabolism phenotype
• *normal* homeostasis/metabolism phenotype (MGI Ref ID J:100328)
  • despite peripheral insulin resistance, mice exhibit normal non-esterified fatty acid levels in the plasma and do not develop diabetes
• decreased circulating leptin level (MGI Ref ID J:100328)
• improved glucose tolerance (MGI Ref ID J:100328)
• increased circulating glucose level (MGI Ref ID J:100328)
  • fasting glucose levels are slightly higher than in wild-type mice
• increased circulating insulin level (MGI Ref ID J:100328)
  • when fed ad libitum and following glucose loading, mice exhibit increased circulating insulin levels
  • mice exhibit postprandial hyperinsulinemia
• insulin resistance (MGI Ref ID J:100328)
  • mice exhibit peripheral insulin resistance
• endocrine/exocrine gland phenotype
• increased pancreatic beta cell mass (MGI Ref ID J:100328)
  • beta cell mass is increased 3-fold compared to in wild-type mice
• pancreatic islet hyperplasia (MGI Ref ID J:100328)
• liver/biliary system phenotype
• enlarged liver (MGI Ref ID J:100328)
• digestive/alimentary phenotype
• increased pancreatic beta cell mass (MGI Ref ID J:100328)
  • beta cell mass is increased 3-fold compared to in wild-type mice
• pancreatic islet hyperplasia (MGI Ref ID J:100328)
• adipose tissue phenotype
• abnormal adipose tissue morphology (MGI Ref ID J:100328)
  • lipodystrophy (loss and abnormal redistribution of body fat)

Tg(SREBP1a)7343Reh/0

        Background Not Specified

• liver/biliary system phenotype
• decreased liver regeneration (MGI Ref ID J:129886)
  • after 70% resection, liver regeneration is suppressed due to decreased proliferation compared to in wild-type mice

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

SREBF1 related

Metabolism Research
Lipid Metabolism

Genes & Alleles

Gene & Allele Information

Allele Symbol		Tg(SREBP1a)7343Reh
Allele Name		transgene insertion 7343, Robert E Hammer
Allele Type		Transgenic (random, expressed)
Common Name(s)		Tg(SREBF1)2Mbjg; TgSREBP-1a;
Mutation Made By		Robert Hammer,   U. of Texas, SW Medical Center at Dallas
Expressed Gene		SREBF1, sterol regulatory element binding transcription factor 1, human
Promoter		Pepck, Pck1-phosphoenolpyruvate carboxykinase,
Molecular Note		The transgene contains human sterol regulatory element binding transcription factor 1 (SREBF1) under control of the phosphoenolpyruvate carboxykinase (Pck1) promoter. Seven lines were created. [MGI Ref ID J:35770]

Genotyping

Genotyping Information

Genotyping Protocols

Human Growth Hormone Polyadenylation Signal Sequence, STD PCR, vers. 1
Tg(SREBP1a)7343Reh, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Shimano H; Horton JD; Hammer RE; Shimomura I; Brown MS; Goldstein JL. 1996. Overproduction of cholesterol and fatty acids causes massive liver enlargement in transgenic mice expressing truncated SREBP-1a [see comments] J Clin Invest 98(7):1575-84. [PubMed: 8833906]  [MGI Ref ID J:35770]

Additional References

Tg(SREBP1a)7343Reh related

Horton JD; Shah NA; Warrington JA; Anderson NN; Park SW; Brown MS; Goldstein JL. 2003. Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes. Proc Natl Acad Sci U S A 100(21):12027-32. [PubMed: 14512514]  [MGI Ref ID J:86192]

Inoue N; Shimano H; Nakakuki M; Matsuzaka T; Nakagawa Y; Yamamoto T; Sato R; Takahashi A; Sone H; Yahagi N; Suzuki H; Toyoshima H; Yamada N. 2005. Lipid synthetic transcription factor SREBP-1a activates p21WAF1/CIP1, a universal cyclin-dependent kinase inhibitor. Mol Cell Biol 25(20):8938-47. [PubMed: 16199872]  [MGI Ref ID J:101351]

Matsuzaka T; Shimano H; Yahagi N; Amemiya-Kudo M; Yoshikawa T; Hasty AH; Tamura Y; Osuga Ji J; Okazaki H; Iizuka Y; Takahashi A; Sone H; Gotoda T; Ishibashi S; Yamada N. 2002. Dual regulation of mouse Delta(5)- and Delta(6)-desaturase gene expression by SREBP-1 and PPARalpha. J Lipid Res 43(1):107-14. [PubMed: 11792729]  [MGI Ref ID J:73909]

Maxwell KN; Soccio RE; Duncan EM; Sehayek E; Breslow JL. 2003. Novel putative SREBP and LXR target genes identified by microarray analysis in liver of cholesterol-fed mice. J Lipid Res 44(11):2109-19. [PubMed: 12897189]  [MGI Ref ID J:93102]

Nakakuki M; Shimano H; Inoue N; Tamura M; Matsuzaka T; Nakagawa Y; Yahagi N; Toyoshima H; Sato R; Yamada N. 2007. A transcription factor of lipid synthesis, sterol regulatory element-binding protein (SREBP)-1a causes G(1) cell-cycle arrest after accumulation of cyclin-dependent kinase (cdk) inhibitors. FEBS J 274(17):4440-52. [PubMed: 17662109]  [MGI Ref ID J:129886]

Sakakura Y; Shimano H; Sone H; Takahashi A; Inoue N; Toyoshima H; Suzuki S; Yamada N. 2001. Sterol regulatory element-binding proteins induce an entire pathway of cholesterol synthesis. Biochem Biophys Res Commun 286(1):176-83. [PubMed: 11485325]  [MGI Ref ID J:127755]

Takahashi A; Shimano H; Nakagawa Y; Yamamoto T; Motomura K; Matsuzaka T; Sone H; Suzuki H; Toyoshima H; Yamada N. 2005. Transgenic mice overexpressing SREBP-1a under the control of the PEPCK promoter exhibit insulin resistance, but not diabetes. Biochim Biophys Acta 1740(3):427-33. [PubMed: 15949711]  [MGI Ref ID J:100328]

Health & husbandry

Health & Colony Maintenance Information

Colony Maintenance

Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information.

Supply Notes

Cryorecovery - Standard. At least two mice that carry the mutation (if it is a mutant strain) will be provided. The total number of animals provided, their gender and genotype will vary. Please inquire if larger numbers of animals with specific genotypes and genders are needed. IMPORTANT NOTE: The genotypes of the animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire for possible genotypes for this specific strain. Animals typically ship within 13 to 16 weeks from your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will typically ship within 25 weeks. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 or 1-207-288-5845. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	Noncarrier
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

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Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

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MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.