Description

Strain Information

Former Names B6;129S4-Fcgr2btm1Rav/J    (Changed: 03-AUG-07 ) Type Mutant Stock; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Homozygote x Homozygote         (Female x Male) Species laboratory mouse Generation F?+18N1F9 (04-DEC-07)   Donating Investigator Jeffrey Ravetch,   Memorial Sloan Kettering Cancer Center

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Fcgr2^tm1Ttk targeted mutation are viable and fertile. Myeloid and lymphoid development is normal. Immunoglobulin levels are elevated in response to thymus-dependent and thymus-independent antigens. Mice are sensitive to IgG-triggered degranulation and have an enhanced passive cutaneous analphylaxis reaction.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Related Strains

Strains carrying   Fcgr2b^tm1Ttk allele

003817

B6;129S-Fcgr2btm1Ttk Clcn1adr-mto5J/J

View Strains carrying   Fcgr2b^tm1Ttk     (1 strain)

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms

Systemic Lupus Erythematosus; SLE - Models with phenotypic similarity to human disease where etiologies involve orthologs.1

1 Human genes are associated with this disease. Orthologs of those genes appear in the mouse genotype(s).

View Mammalian Phenotype Terms

Mammalian Phenotype Terms

      assigned by genotype

The following phenotype information may relate to a genetic background differing from this JAX^® Mice strain.

Fcgr2b^tm1Ttk/Fcgr2b^tm1Ttk

        involves: 129S4/SvJae

• immune system phenotype
• abnormal mast cell physiology (MGI Ref ID J:31133)
  • mast cells highly sensitive to IgG-triggered degranulation as compared to wild-type littermate controls
• increased IgA level (MGI Ref ID J:31133)
  • elevated IgA levels in response to both T-dependent and T-independent antigens
• increased IgG level (MGI Ref ID J:31133)
  • elevated IgG1, IgG2a, IgG2b, IgG3 levels in response to both T-dependent and T-independent antigens
• increased IgG1 level (MGI Ref ID J:31133)
• increased IgG2a level (MGI Ref ID J:31133)
• increased IgG2b level (MGI Ref ID J:31133)
• increased IgG3 level (MGI Ref ID J:31133)
• increased IgM level (MGI Ref ID J:31133)
  • elevated IgM levels in response to both T-dependent and T-independent antigens
• increased susceptibility to type I hypersensitivity reaction (MGI Ref ID J:31133)
  • enhanced response to IgG1-dependent passive systemic anaphylaxis, however development of myeloid and lymphoid cell lineages was normal

Fcgr2b^tm1Ttk/Fcgr2b^tm1Ttk

        C.129S4-Fcgr2b^tm1Ttk

• normal phenotype
• no abnormal phenotype detected (MGI Ref ID J:64152)
  • appeared healthy and had life spans comparable to wild type littermates appeared healthy and had life spans comparable to wild-type littermates

Fcgr2b^tm1Ttk/Fcgr2b^tm1Ttk

        B6.129S4-Fcgr2b^tm1Ttk

• life span-post-weaning/aging
• premature death (MGI Ref ID J:64152)
  • death by 9 months, with evidence of clear distress, including: hunched posture, edema, dehydration
• behavior/neurological phenotype
• hunched posture (MGI Ref ID J:64152)
• digestive/alimentary phenotype
• pancreas inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the pancreas
• salivary gland inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the salivary gland
• endocrine/exocrine gland phenotype
• pancreas inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the pancreas
• salivary gland inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the salivary gland
• growth/size phenotype
• decreased body weight (MGI Ref ID J:64152)
  • reduced weight, evident with signs of distress near death
• hematopoietic system phenotype
• anemia (MGI Ref ID J:64152)
  • evident at 8 months of age
• enlarged spleen (MGI Ref ID J:64152)
• homeostasis/metabolism phenotype
• dehydration (MGI Ref ID J:64152)
• edema (MGI Ref ID J:64152)
• proteinuria (MGI Ref ID J:64152)
  • evident beginning at 5 months of age in 40% of mice and in 90% of mice at 9 months of age
• immune system phenotype
• enlarged lymph nodes (MGI Ref ID J:64152)
• enlarged spleen (MGI Ref ID J:64152)
• hypersensitivity (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed severe multiorgan inflammatory disease
• increased immunoglobulin level (MGI Ref ID J:64152)
  • high serum titers of antinuclear antibodies (ANA)
• increased inflammatory response (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed severe multiorgan inflammatory disease with inflammatory cell infiltration in the lungs, kidneys, salivary glands, pancreas, liver, tongue, and muscle
• kidney inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the kidney
• glomerulonephritis (MGI Ref ID J:64152)
  • evident at 8 months
• liver inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the liver
• lung inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the lung
• pancreas inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the pancreas
• salivary gland inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the salivary gland
• vasculitis (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed systemic vasculitis
• renal/urinary system phenotype
• kidney inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the kidney
• glomerulonephritis (MGI Ref ID J:64152)
  • evident at 8 months
• proteinuria (MGI Ref ID J:64152)
  • evident beginning at 5 months of age in 40% of mice and in 90% of mice at 9 months of age
• respiratory system phenotype
• lung inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the lung
• cardiovascular system phenotype
• vasculitis (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed systemic vasculitis
• liver/biliary system phenotype
• liver inflammation (MGI Ref ID J:64152)
  • pathological examination at 8 months of age showed inflammatory cell infiltration in the liver

View Research Applications

Research Applications

This mouse can be used to support research in many areas including:

Fcgr2b^tm1Ttk related

Immunology and Inflammation Research
CD Antigens, Antigen Receptors, and Histocompatibility Markers
Inflammation

Genes & Alleles

Gene & Allele Information

Allele Symbol		Fcgr2btm1Ttk
Allele Name		targeted mutation 1, Toshiyuki Takai
Allele Type		Targeted (knock-out)
Common Name(s)		FcgammaRII; FcgammaRII-; FcgammaRII2B-; FcgammaRIIB-; Fcgr2btm1Rav; IIB-;
Mutation Made By		Jeffrey Ravetch,   Memorial Sloan Kettering Cancer Center
Strain of Origin		129S4/SvJae
ES Cell Line Name		J1
ES Cell Line Strain		129S4/SvJae
Gene Symbol and Name		Fcgr2b, Fc receptor, IgG, low affinity IIb
Chromosome		1
Gene Common Name(s)		AI528646; CD32; CD32B; F630109E10Rik; FCG2; FCGR2; Fc gamma RIIB; Fc receptor, IgG, low affinity II; Fc receptor, IgG, low affinity IIa; Fc-gamma receptor 2; Fc-gamma receptor 3; Fc[g]RII; FcgRII; FcgammaRIIB; Fcgr2; Fcgr2a; Fcr-2; Fcr-3; IGFR2; Ly-17; Ly-m20; LyM-1; RIKEN cDNA F630109E10 gene; expressed sequence AI528646; lymphocyte antigen 17; lymphocyte antigen m20;
Molecular Note		A 2.3 kb genomic fragment containing the S1 and EC1 exons was replaced by a neomycin resistance cassette. Encoded transcript was undetectable in homozygous mutant mice via Northern blot analysis (data not shown). Western blot analysis of LPS-stimulatedB cells derived from homozygous mice confirmed that no protein was made from this allele, and FACS analysis demonstrated that no functional protein was expressed on the cell surface of B cells in homozygous mice. [MGI Ref ID J:31133]

Genotyping

Genotyping Information

Genotyping Protocols

Fcgr2b^tm1Ttk, STD PCR, vers. 1

Helpful Links

Optimizing PCR Protocols

References

References

Selected Reference(s)

Takai T; Ono M; Hikida M; Ohmori H; Ravetch JV. 1996. Augmented humoral and anaphylactic responses in Fc gamma RII-deficient mice. Nature 379(6563):346-9. [PubMed: 8552190]  [MGI Ref ID J:31133]

Additional References

Ji H; Ohmura K; Mahmood U; Lee DM; Hofhuis FM; Boackle SA; Takahashi K; Holers VM; Walport M; Gerard C; Ezekowitz A; Carroll MC; Brenner M; Weissleder R; Verbeek JS; Duchatelle V; Degott C; Benoist C; Mathis D. 2002. Arthritis critically dependent on innate immune system players. Immunity 16(2):157-68. [PubMed: 11869678]  [MGI Ref ID J:74720]

Fcgr2b^tm1Ttk related

Anders HJ; Krug A; Pawar RD. 2008. Molecular mimicry in innate immunity? The viral RNA recognition receptor TLR7 accelerates murine lupus. Eur J Immunol 38(7):1795-9. [PubMed: 18581336]  [MGI Ref ID J:137453]

Bandukwala HS; Clay BS; Tong J; Mody PD; Cannon JL; Shilling RA; Verbeek JS; Weinstock JV; Solway J; Sperling AI. 2007. Signaling through Fc gamma RIII is required for optimal T helper type (Th)2 responses and Th2-mediated airway inflammation. J Exp Med 204(8):1875-89. [PubMed: 17664287]  [MGI Ref ID J:125951]

Bazin R; Lemieux R; Tremblay T. 2006. Reversal of immune thrombocytopenia in mice by cross-linking human immunoglobulin G with a high-affinity monoclonal antibody. Br J Haematol 135(1):97-100. [PubMed: 16925577]  [MGI Ref ID J:112878]

Bergtold A; Desai DD; Gavhane A; Clynes R. 2005. Cell surface recycling of internalized antigen permits dendritic cell priming of B cells. Immunity 23(5):503-14. [PubMed: 16286018]  [MGI Ref ID J:113283]

Bolland S; Ravetch JV. 2000. Spontaneous autoimmune disease in Fc(gamma)RIIB-deficient mice results from strain-specific epistasis Immunity 13(2):277-85. [PubMed: 10981970]  [MGI Ref ID J:64152]

Bolland S; Yim YS; Tus K; Wakeland EK; Ravetch JV. 2002. Genetic modifiers of systemic lupus erythematosus in FcgammaRIIB(-/-) mice. J Exp Med 195(9):1167-74. [PubMed: 11994421]  [MGI Ref ID J:76486]

Boross P; van Lent PL; Martin-Ramirez J; van der Kaa J; Mulder MH; Claassens JW; van den Berg WB; Arandhara VL; Verbeek JS. 2008. Destructive Arthritis in the Absence of Both Fc{gamma}RI and Fc{gamma}RIII. J Immunol 180(7):5083-91. [PubMed: 18354234]  [MGI Ref ID J:133367]

Boule MW; Broughton C; Mackay F; Akira S; Marshak-Rothstein A; Rifkin IR. 2004. Toll-like receptor 9-dependent and -independent dendritic cell activation by chromatin-immunoglobulin G complexes. J Exp Med 199(12):1631-40. [PubMed: 15197227]  [MGI Ref ID J:120392]

Brownlie RJ; Lawlor KE; Niederer HA; Cutler AJ; Xiang Z; Clatworthy MR; Floto RA; Greaves DR; Lyons PA; Smith KG. 2008. Distinct cell-specific control of autoimmunity and infection by FcgammaRIIb. J Exp Med 205(4):883-95. [PubMed: 18362174]  [MGI Ref ID J:133975]

Charles JF; Humphrey MB; Zhao X; Quarles E; Nakamura MC; Aderem A; Seaman WE; Smith KD. 2008. The innate immune response to Salmonella enterica serovar Typhimurium by macrophages is dependent on TREM2-DAP12. Infect Immun 76(6):2439-47. [PubMed: 18391000]  [MGI Ref ID J:136148]

Clatworthy MR; Smith KG. 2004. FcgammaRIIb balances efficient pathogen clearance and the cytokine-mediated consequences of sepsis. J Exp Med 199(5):717-23. [PubMed: 14981111]  [MGI Ref ID J:124296]

Clatworthy MR; Willcocks L; Urban B; Langhorne J; Williams TN; Peshu N; Watkins NA; Floto RA; Smith KG. 2007. Systemic lupus erythematosus-associated defects in the inhibitory receptor Fc{gamma}RIIb reduce susceptibility to malaria. Proc Natl Acad Sci U S A 104(17):7169-74. [PubMed: 17435165]  [MGI Ref ID J:120905]

Clynes R; Maizes JS; Guinamard R; Ono M; Takai T; Ravetch JV. 1999. Modulation of immune complex-induced inflammation in vivo by the coordinate expression of activation and inhibitory Fc receptors. J Exp Med 189(1):179-85. [PubMed: 9874574]  [MGI Ref ID J:112378]

Clynes RA; Towers TL; Presta LG; Ravetch JV. 2000. Inhibitory Fc receptors modulate in vivo cytoxicity against tumor targets. Nat Med 6(4):443-6. [PubMed: 10742152]  [MGI Ref ID J:118052]

Corr M; Crain B. 2002. The role of FcgammaR signaling in the K/B x N serum transfer model of arthritis. J Immunol 169(11):6604-9. [PubMed: 12444173]  [MGI Ref ID J:124384]

Diaz de Stahl T; Dahlstrom J; Carroll MC; Heyman B. 2003. A role for complement in feedback enhancement of antibody responses by IgG3. J Exp Med 197(9):1183-90. [PubMed: 12732659]  [MGI Ref ID J:83277]

El Shikh ME; El Sayed R; Szakal AK; Tew JG. 2006. Follicular dendritic cell (FDC)-FcgammaRIIB engagement via immune complexes induces the activated FDC phenotype associated with secondary follicle development. Eur J Immunol 36(10):2715-24. [PubMed: 17013985]  [MGI Ref ID J:117020]

Feng HM; Walker DH. 2004. Mechanisms of immunity to Ehrlichia muris: a model of monocytotropic ehrlichiosis. Infect Immun 72(2):966-71. [PubMed: 14742542]  [MGI Ref ID J:87862]

Fukuyama H; Nimmerjahn F; Ravetch JV. 2005. The inhibitory Fcgamma receptor modulates autoimmunity by limiting the accumulation of immunoglobulin G+ anti-DNA plasma cells. Nat Immunol 6(1):99-106. [PubMed: 15592473]  [MGI Ref ID J:94871]

Getahun A; Dahlstrom J; Wernersson S; Heyman B. 2004. IgG2a-mediated enhancement of antibody and T cell responses and its relation to inhibitory and activating Fc gamma receptors. J Immunol 172(9):5269-76. [PubMed: 15100265]  [MGI Ref ID J:89643]

Gjertsson I; Kleinau S; Tarkowski A. 2002. The impact of Fcgamma receptors on Staphylococcus aureus infection. Microb Pathog 33(4):145-52. [PubMed: 12385742]  [MGI Ref ID J:102982]

Godau J; Heller T; Hawlisch H; Trappe M; Howells E; Best J; Zwirner J; Verbeek JS; Hogarth PM; Gerard C; Van Rooijen N; Klos A; Gessner JE; Kohl J. 2004. C5a initiates the inflammatory cascade in immune complex peritonitis. J Immunol 173(5):3437-45. [PubMed: 15322209]  [MGI Ref ID J:92709]

Hamaguchi Y; Xiu Y; Komura K; Nimmerjahn F; Tedder TF. 2006. Antibody isotype-specific engagement of Fcgamma receptors regulates B lymphocyte depletion during CD20 immunotherapy. J Exp Med 203(3):743-53. [PubMed: 16520392]  [MGI Ref ID J:123761]

Harbers SO; Crocker A; Catalano G; D'Agati V; Jung S; Desai DD; Clynes R. 2007. Antibody-enhanced cross-presentation of self antigen breaks T cell tolerance. J Clin Invest 117(5):1361-9. [PubMed: 17446931]  [MGI Ref ID J:122114]

Ioan-Facsinay A; de Kimpe SJ; Hellwig SM; van Lent PL; Hofhuis FM; van Ojik HH; Sedlik C; da Silveira SA; Gerber J; de Jong YF; Roozendaal R; Aarden LA; van den Berg WB; Saito T; Mosser D; Amigorena S; Izui S; van Ommen GJ; van Vugt M; van de Winkel JG; Verbeek JS. 2002. FcgammaRI (CD64) contributes substantially to severity of arthritis, hypersensitivity responses, and protection from bacterial infection. Immunity 16(3):391-402. [PubMed: 11911824]  [MGI Ref ID J:83995]

Iruretagoyena MI; Riedel CA; Leiva ED; Gutierrez MA; Jacobelli SH; Kalergis AM. 2008. Activating and inhibitory Fcgamma receptors can differentially modulate T cell-mediated autoimmunity. Eur J Immunol 38(8):2241-50. [PubMed: 18604868]  [MGI Ref ID J:138199]

Ji H; Ohmura K; Mahmood U; Lee DM; Hofhuis FM; Boackle SA; Takahashi K; Holers VM; Walport M; Gerard C; Ezekowitz A; Carroll MC; Brenner M; Weissleder R; Verbeek JS; Duchatelle V; Degott C; Benoist C; Mathis D. 2002. Arthritis critically dependent on innate immune system players. Immunity 16(2):157-68. [PubMed: 11869678]  [MGI Ref ID J:74720]

Jodo S; Kung JT; Xiao S; Chan DV; Kobayashi S; Tateno M; Lafyatis R; Ju ST. 2003. Anti-CD95-induced lethality requires radioresistant Fcgamma RII+ cells. A novel mechanism for fulminant hepatic failure. J Biol Chem 278(9):7553-7. [PubMed: 12477718]  [MGI Ref ID J:82143]

Kaplan CD; O'Neill SK; Koreny T; Czipri M; Finnegan A. 2002. Development of inflammation in proteoglycan-induced arthritis is dependent on FcgammaR regulation of the cytokine/chemokine environment. J Immunol 169(10):5851-9. [PubMed: 12421967]  [MGI Ref ID J:80076]

Karlsson MC; Getahun A; Heyman B. 2001. FcgammaRIIB in IgG-Mediated Suppression of Antibody Responses: Different Impact In Vivo and In Vitro. J Immunol 167(10):5558-64. [PubMed: 11698426]  [MGI Ref ID J:72678]

Kikuchi S; Santiago-Raber ML; Amano H; Amano E; Fossati-Jimack L; Moll T; Kotzin BL; Izui S. 2006. Contribution of NZB autoimmunity 2 to Y-linked autoimmune acceleration-induced monocytosis in association with murine systemic lupus. J Immunol 176(5):3240-7. [PubMed: 16493085]  [MGI Ref ID J:129409]

Kirimanjeswara GS; Mann PB; Pilione M; Kennett MJ; Harvill ET. 2005. The complex mechanism of antibody-mediated clearance of Bordetella from the lungs requires TLR4. J Immunol 175(11):7504-11. [PubMed: 16301658]  [MGI Ref ID J:122156]

Klein MA; Kaeser PS; Schwarz P; Weyd H; Xenarios I; Zinkernagel RM; Carroll MC; Verbeek JS; Botto M; Walport MJ; Molina H; Kalinke U; Acha-Orbea H; Aguzzi A. 2001. Complement facilitates early prion pathogenesis. Nat Med 7(4):488-92. [PubMed: 11283678]  [MGI Ref ID J:125467]

Kleinau S; Martinsson P; Heyman B. 2000. Induction and suppression of collagen-induced arthritis is dependent on distinct fcgamma receptors. J Exp Med 191(9):1611-6. [PubMed: 10790435]  [MGI Ref ID J:61920]

Kowalewska J; Muhlfeld AS; Hudkins KL; Yeh MM; Farr AG; Ravetch JV; Alpers CE. 2007. Thymic stromal lymphopoietin transgenic mice develop cryoglobulinemia and hepatitis with similarities to human hepatitis C liver disease. Am J Pathol 170(3):981-9. [PubMed: 17322382]  [MGI Ref ID J:118658]

Kumar V; Ali SR; Konrad S; Zwirner J; Verbeek JS; Schmidt RE; Gessner JE. 2006. Cell-derived anaphylatoxins as key mediators of antibody-dependent type II autoimmunity in mice. J Clin Invest 116(2):512-20. [PubMed: 16453025]  [MGI Ref ID J:105421]

Lajaunias F; Nitschke L; Moll T; Martinez-Soria E; Semac I; Chicheportiche Y; Parkhouse RM; Izui S. 2002. Differentially regulated expression and function of CD22 in activated B-1 and B-2 lymphocytes. J Immunol 168(12):6078-83. [PubMed: 12055217]  [MGI Ref ID J:123796]

Li J; Tuzun E; Wu XR; Qi HB; Allman W; Saini SS; Christadoss P. 2008. Inhibitory IgG receptor FcgammaRIIB fails to inhibit experimental autoimmune myasthenia gravis pathogenesis. J Neuroimmunol 194(1-2):44-53. [PubMed: 18207575]  [MGI Ref ID J:131899]

Li N; Zhao M; Hilario-Vargas J; Prisayanh P; Warren S; Diaz LA; Roopenian DC; Liu Z. 2005. Complete FcRn dependence for intravenous Ig therapy in autoimmune skin blistering diseases. J Clin Invest 115(12):3440-50. [PubMed: 16284651]  [MGI Ref ID J:104709]

Liu H; Shalev I; Manuel J; He W; Leung E; Crookshank J; Liu MF; Diao J; Cattral M; Clark DA; Isenman DE; Gorczynski RM; Grant DR; Zhang L; Phillips MJ; Cybulsky MI; Levy GA. 2008. The FGL2-FcgammaRIIB pathway: A novel mechanism leading to immunosuppression. Eur J Immunol 38(11):3114-26. [PubMed: 18991288]  [MGI Ref ID J:141402]

Lowe DB; Shearer MH; Jumper CA; Bright RK; Kennedy RC. 2007. Fc gamma receptors play a dominant role in protective tumor immunity against a virus-encoded tumor-specific antigen in a murine model of experimental pulmonary metastases. J Virol 81(3):1313-8. [PubMed: 17108042]  [MGI Ref ID J:117823]

Maglione PJ; Xu J; Casadevall A; Chan J. 2008. Fc{gamma} Receptors Regulate Immune Activation and Susceptibility during Mycobacterium tuberculosis Infection. J Immunol 180(5):3329-38. [PubMed: 18292558]  [MGI Ref ID J:131525]

Martinsson K; Hultman P. 2006. The role of Fc-receptors in murine mercury-induced systemic autoimmunity. Clin Exp Immunol 144(2):309-18. [PubMed: 16634805]  [MGI Ref ID J:108127]

Masuda A; Nakamura A; Maeda T; Sakamoto Y; Takai T. 2007. Cis binding between inhibitory receptors and MHC class I can regulate mast cell activation. J Exp Med 204(4):907-20. [PubMed: 17420263]  [MGI Ref ID J:125576]

Masuda A; Yoshida M; Shiomi H; Ikezawa S; Takagawa T; Tanaka H; Chinzei R; Ishida T; Morita Y; Kutsumi H; Inokuchi H; Wang S; Kobayashi K; Mizuno S; Nakamura A; Takai T; Blumberg RS; Azuma T. 2008. Fcgamma receptor regulation of Citrobacter rodentium infection. Infect Immun 76(4):1728-37. [PubMed: 18227164]  [MGI Ref ID J:133533]

McGaha TL; Karlsson MC; Ravetch JV. 2008. FcgammaRIIB deficiency leads to autoimmunity and a defective response to apoptosis in Mrl-MpJ mice. J Immunol 180(8):5670-9. [PubMed: 18390752]  [MGI Ref ID J:134233]

Menager N; Foster G; Ugrinovic S; Uppington H; Verbeek S; Mastroeni P. 2007. Fcgamma receptors are crucial for the expression of acquired resistance to virulent Salmonella enterica serovar Typhimurium in vivo but are not required for the induction of humoral or T-cell-mediated immunity. Immunology 120(3):424-32. [PubMed: 17328787]  [MGI Ref ID J:122709]

Minard-Colin V; Xiu Y; Poe JC; Horikawa M; Magro CM; Hamaguchi Y; Haas KM; Tedder TF. 2008. Lymphoma depletion during CD20 immunotherapy in mice is mediated by macrophage FcgammaRI, FcgammaRIII, and FcgammaRIV. Blood 112(4):1205-13. [PubMed: 18495955]  [MGI Ref ID J:138431]

Mold C; Rodic-Polic B; Du Clos TW. 2002. Protection from Streptococcus pneumoniae infection by C-reactive protein and natural antibody requires complement but not Fc gamma receptors. J Immunol 168(12):6375-81. [PubMed: 12055255]  [MGI Ref ID J:123793]

Mold C; Rodriguez W; Rodic-Polic B; Du Clos TW. 2002. C-reactive protein mediates protection from lipopolysaccharide through interactions with Fc gamma R. J Immunol 169(12):7019-25. [PubMed: 12471137]  [MGI Ref ID J:118418]

Moll T; Nitschke L; Carroll M; Ravetch JV; Izui S. 2004. A critical role for Fc gamma RIIB in the induction of rheumatoid factors. J Immunol 173(7):4724-8. [PubMed: 15383609]  [MGI Ref ID J:93718]

Moore T; Ananaba GA; Bolier J; Bowers S; Belay T; Eko FO; Igietseme JU. 2002. Fc receptor regulation of protective immunity against Chlamydia trachomatis. Immunology 105(2):213-21. [PubMed: 11872097]  [MGI Ref ID J:113515]

Moore T; Ekworomadu CO; Eko FO; MacMillan L; Ramey K; Ananaba GA; Patrickson JW; Nagappan PR; Lyn D; Black CM; Igietseme JU. 2003. Fc receptor-mediated antibody regulation of T cell immunity against intracellular pathogens. J Infect Dis 188(4):617-24. [PubMed: 12898452]  [MGI Ref ID J:120672]

Muhlfeld AS; Segerer S; Hudkins K; Carling MD; Wen M; Farr AG; Ravetch JV; Alpers CE. 2003. Deletion of the fcgamma receptor IIb in thymic stromal lymphopoietin transgenic mice aggravates membranoproliferative glomerulonephritis. Am J Pathol 163(3):1127-36. [PubMed: 12937154]  [MGI Ref ID J:85173]

Nabbe KC; Blom AB; Holthuysen AE; Boross P; Roth J; Verbeek S; van Lent PL; van den Berg WB. 2003. Coordinate expression of activating Fc gamma receptors I and III and inhibiting Fc gamma receptor type II in the determination of joint inflammation and cartilage destruction during immune complex-mediated arthritis. Arthritis Rheum 48(1):255-65. [PubMed: 12528127]  [MGI Ref ID J:106191]

Nakamura A; Nukiwa T; Takai T. 2003. Deregulation of peripheral B-cell development in enhanced severity of collagen-induced arthritis in FcgammaRIIB-deficient mice. J Autoimmun 20(3):227-36. [PubMed: 12753808]  [MGI Ref ID J:119452]

Nakamura A; Yuasa T; Ujike A; Ono M; Nukiwa T; Ravetch JV; Takai T. 2000. Fcgamma receptor IIB-deficient mice develop Goodpasture's syndrome upon immunization with type IV collagen: a novel murine model for autoimmune glomerular basement membrane disease. J Exp Med 191(5):899-906. [PubMed: 10704470]  [MGI Ref ID J:60920]

Nakamura K; Hirai H; Torashima T; Miyazaki T; Tsurui H; Xiu Y; Ohtsuji M; Lin QS; Tsukamoto K; Nishimura H; Ono M; Watanabe M; Hirose S. 2007. CD3 and immunoglobulin G Fc receptor regulate cerebellar functions. Mol Cell Biol 27(14):5128-34. [PubMed: 17502348]  [MGI Ref ID J:123637]

Nakamura K; Malykhin A; Coggeshall KM. 2002. The Src homology 2 domain-containing inositol 5-phosphatase negatively regulates Fcgamma receptor-mediated phagocytosis through immunoreceptor tyrosine-based activation motif-bearing phagocytic receptors. Blood 100(9):3374-82. [PubMed: 12384440]  [MGI Ref ID J:115560]

Nigrovic PA; Binstadt BA; Monach PA; Johnsen A; Gurish M; Iwakura Y; Benoist C; Mathis D; Lee DM. 2007. Mast cells contribute to initiation of autoantibody-mediated arthritis via IL-1. Proc Natl Acad Sci U S A 104(7):2325-30. [PubMed: 17277081]  [MGI Ref ID J:119744]

Nimmerjahn F; Bruhns P; Horiuchi K; Ravetch JV. 2005. FcgammaRIV: a novel FcR with distinct IgG subclass specificity. Immunity 23(1):41-51. [PubMed: 16039578]  [MGI Ref ID J:100540]

Nimmerjahn F; Ravetch JV. 2005. Divergent immunoglobulin g subclass activity through selective Fc receptor binding. Science 310(5753):1510-2. [PubMed: 16322460]  [MGI Ref ID J:103229]

Nishimura H; Strominger JL. 2006. Involvement of a tissue-specific autoantibody in skin disorders of murine systemic lupus erythematosus and autoinflammatory diseases. Proc Natl Acad Sci U S A 103(9):3292-7. [PubMed: 16492738]  [MGI Ref ID J:107174]

Obukhanych TV; Nussenzweig MC. 2006. T-independent type II immune responses generate memory B cells. J Exp Med 203(2):305-10. [PubMed: 16476769]  [MGI Ref ID J:119148]

Okazaki T; Otaka Y; Wang J; Hiai H; Takai T; Ravetch JV; Honjo T. 2005. Hydronephrosis associated with antiurothelial and antinuclear autoantibodies in BALB/c-Fcgr2b-/-Pdcd1-/- mice. J Exp Med 202(12):1643-8. [PubMed: 16352741]  [MGI Ref ID J:118829]

Park-Min KH; Serbina NV; Yang W; Ma X; Krystal G; Neel BG; Nutt SL; Hu X; Ivashkiv LB. 2007. FcgammaRIII-dependent inhibition of interferon-gamma responses mediates suppressive effects of intravenous immune globulin. Immunity 26(1):67-78. [PubMed: 17239631]  [MGI Ref ID J:118322]

Paul E; Nelde A; Verschoor A; Carroll MC. 2007. Follicular exclusion of autoreactive B cells requires Fc{gamma}RIIb. Int Immunol 19(4):365-73. [PubMed: 17307801]  [MGI Ref ID J:119987]

Petkova SB; Akilesh S; Sproule TJ; Christianson GJ; Al Khabbaz H; Brown AC; Presta LG; Meng YG; Roopenian DC. 2006. Enhanced half-life of genetically engineered human IgG1 antibodies in a humanized FcRn mouse model: potential application in humorally mediated autoimmune disease. Int Immunol 18(12):1759-69. [PubMed: 17077181]  [MGI Ref ID J:133048]

Petkova SB; Konstantinov KN; Sproule TJ; Lyons BL; Awwami MA; Roopenian DC. 2006. Human antibodies induce arthritis in mice deficient in the low-affinity inhibitory IgG receptor Fc gamma RIIB. J Exp Med 203(2):275-80. [PubMed: 16476768]  [MGI Ref ID J:118959]

Pinheiro da Silva F; Aloulou M; Skurnik D; Benhamou M; Andremont A; Velasco IT; Chiamolera M; Verbeek JS; Launay P; Monteiro RC. 2007. CD16 promotes Escherichia coli sepsis through an FcR gamma inhibitory pathway that prevents phagocytosis and facilitates inflammation. Nat Med 13(11):1368-74. [PubMed: 17934470]  [MGI Ref ID J:130075]

Pisitkun P; Deane JA; Difilippantonio MJ; Tarasenko T; Satterthwaite AB; Bolland S. 2006. Autoreactive B cell responses to RNA-related antigens due to TLR7 gene duplication. Science 312(5780):1669-72. [PubMed: 16709748]  [MGI Ref ID J:109758]

Qin D; Wu J; Vora KA; Ravetch JV; Szakal AK; Manser T; Tew JG. 2000. Fc gamma receptor IIB on follicular dendritic cells regulates the B cell recall response. J Immunol 164(12):6268-75. [PubMed: 10843680]  [MGI Ref ID J:112126]

Rahman ZS; Alabyev B; Manser T. 2007. FcgammaRIIB regulates autoreactive primary antibody-forming cell, but not germinal center B cell, activity. J Immunol 178(2):897-907. [PubMed: 17202351]  [MGI Ref ID J:142628]

Rahman ZS; Manser T. 2005. Failed up-regulation of the inhibitory IgG Fc receptor Fc gamma RIIB on germinal center B cells in autoimmune-prone mice is not associated with deletion polymorphisms in the promoter region of the Fc gamma RIIB gene. J Immunol 175(3):1440-9. [PubMed: 16034080]  [MGI Ref ID J:107299]

Rao SP; Vora KA; Manser T. 2002. Differential expression of the inhibitory IgG Fc receptor FcgammaRIIB on germinal center cells: implications for selection of high-affinity B cells. J Immunol 169(4):1859-68. [PubMed: 12165510]  [MGI Ref ID J:112043]

Saeland E; Vidarsson G; Leusen JH; Van Garderen E; Nahm MH; Vile-Weekhout H; Walraven V; Stemerding AM; Verbeek JS; Rijkers GT; Kuis W; Sanders EA; Van De Winkel JG. 2003. Central role of complement in passive protection by human IgG1 and IgG2 anti-pneumococcal antibodies in mice. J Immunol 170(12):6158-64. [PubMed: 12794146]  [MGI Ref ID J:83712]

Samsom JN; van Berkel LA; van Helvoort JM; Unger WW; Jansen W; Thepen T; Mebius RE; Verbeek SS; Kraal G. 2005. FcgammaRIIB regulates nasal and oral tolerance: a role for dendritic cells. J Immunol 174(9):5279-87. [PubMed: 15843524]  [MGI Ref ID J:98421]

Schubert D; Maier B; Morawietz L; Krenn V; Kamradt T. 2004. Immunization with glucose-6-phosphate isomerase induces T cell-dependent peripheral polyarthritis in genetically unaltered mice. J Immunol 172(7):4503-9. [PubMed: 15034067]  [MGI Ref ID J:88723]

Schuurhuis DH; van Montfoort N; Ioan-Facsinay A; Jiawan R; Camps M; Nouta J; Melief CJ; Verbeek JS; Ossendorp F. 2006. Immune complex-loaded dendritic cells are superior to soluble immune complexes as antitumor vaccine. J Immunol 176(8):4573-80. [PubMed: 16585547]  [MGI Ref ID J:131164]

Shalev I; Liu H; Koscik C; Bartczak A; Javadi M; Wong KM; Maknojia A; He W; Liu MF; Diao J; Winter E; Manuel J; McCarthy D; Cattral M; Gommerman J; Clark DA; Phillips MJ; Gorczynski RR; Zhang L; Downey G; Grant D; Cybulsky MI; Levy G. 2008. Targeted deletion of fgl2 leads to impaired regulatory T cell activity and development of autoimmune glomerulonephritis. J Immunol 180(1):249-60. [PubMed: 18097026]  [MGI Ref ID J:130895]

Sitaru C; Chiriac MT; Mihai S; Buning J; Gebert A; Ishiko A; Zillikens D. 2006. Induction of complement-fixing autoantibodies against type VII collagen results in subepidermal blistering in mice. J Immunol 177(5):3461-8. [PubMed: 16920988]  [MGI Ref ID J:139521]

Skokowa J; Ali SR; Felda O; Kumar V; Konrad S; Shushakova N; Schmidt RE; Piekorz RP; Nurnberg B; Spicher K; Birnbaumer L; Zwirner J; Claassens JW; Verbeek JS; van Rooijen N; Kohl J; Gessner JE. 2005. Macrophages induce the inflammatory response in the pulmonary Arthus reaction through G alpha i2 activation that controls C5aR and Fc receptor cooperation. J Immunol 174(5):3041-50. [PubMed: 15728518]  [MGI Ref ID J:97718]

Stein MP; Mold C; Du Clos TW. 2000. C-reactive protein binding to murine leukocytes requires Fc gamma receptors. J Immunol 164(3):1514-20. [PubMed: 10640769]  [MGI Ref ID J:77127]

Strait RT; Morris SC; Finkelman FD. 2006. IgG-blocking antibodies inhibit IgE-mediated anaphylaxis in vivo through both antigen interception and Fc gamma RIIb cross-linking. J Clin Invest 116(3):833-41. [PubMed: 16498503]  [MGI Ref ID J:106571]

Sutterwala FS; Noel GJ; Salgame P; Mosser DM. 1998. Reversal of proinflammatory responses by ligating the macrophage Fcgamma receptor type I. J Exp Med 188(1):217-22. [PubMed: 9653099]  [MGI Ref ID J:111539]

Tarasenko T; Kole HK; Bolland S. 2008. A Lupus-Suppressor BALB/c Locus Restricts IgG2 Autoantibodies without Altering Intrinsic B Cell-Tolerance Mechanisms. J Immunol 180(6):3807-14. [PubMed: 18322187]  [MGI Ref ID J:133034]

Tian J; Avalos AM; Mao SY; Chen B; Senthil K; Wu H; Parroche P; Drabic S; Golenbock D; Sirois C; Hua J; An LL; Audoly L; La Rosa G; Bierhaus A; Naworth P; Marshak-Rothstein A; Crow MK; Fitzgerald KA; Latz E; Kiener PA; Coyle AJ. 2007. Toll-like receptor 9-dependent activation by DNA-containing immune complexes is mediated by HMGB1 and RAGE. Nat Immunol 8(5):487-96. [PubMed: 17417641]  [MGI Ref ID J:122427]

Trcka J; Moroi Y; Clynes RA; Goldberg SM; Bergtold A; Perales MA; Ma M; Ferrone CR; Carroll MC; Ravetch JV; Houghton AN. 2002. Redundant and alternative roles for activating Fc receptors and complement in an antibody-dependent model of autoimmune vitiligo. Immunity 16(6):861-8. [PubMed: 12121667]  [MGI Ref ID J:113538]

Ujike A; Ishikawa Y; Ono M; Yuasa T; Yoshino T; Fukumoto M; Ravetch JV; Takai T. 1999. Modulation of immunoglobulin (Ig)E-mediated systemic anaphylaxis by low-affinity Fc receptors for IgG. J Exp Med 189(10):1573-9. [PubMed: 10330436]  [MGI Ref ID J:107371]

Van Lent PL; Nabbe K; Blom AB; Holthuysen AE; Sloetjes A; Van De Putte LB; Verbeek S; Van Den Berg WB. 2001. Role of Activatory FcgammaRI and FcgammaRIII and Inhibitory FcgammaRII in Inflammation and Cartilage Destruction during Experimental Antigen-Induced Arthritis. Am J Pathol 159(6):2309-20. [PubMed: 11733380]  [MGI Ref ID J:72937]

Vines CM; Potter JW; Xu Y; Geahlen RL; Costello PS; Tybulewicz VL; Lowell CA; Chang PW; Gresham HD; Willman CL. 2001. Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr. Immunity 15(4):507-19. [PubMed: 11672534]  [MGI Ref ID J:110529]

Walker JA; Smith KG. 2008. Dependence of surface monoclonal antibody binding on dynamic changes in FcgammaRIIb expression. Immunology 124(3):412-8. [PubMed: 18217946]  [MGI Ref ID J:138471]

Wermeling F; Chen Y; Pikkarainen T; Scheynius A; Winqvist O; Izui S; Ravetch JV; Tryggvason K; Karlsson MC. 2007. Class A scavenger receptors regulate tolerance against apoptotic cells, and autoantibodies against these receptors are predictive of systemic lupus. J Exp Med 204(10):2259-65. [PubMed: 17893199]  [MGI Ref ID J:126041]

Wernersson S; Karlsson MC; Dahlstrom J; Mattsson R; Verbeek JS; Heyman B. 1999. IgG-mediated enhancement of antibody responses is low in Fc receptor gamma chain-deficient mice and increased in Fc gamma RII-deficient mice. J Immunol 163(2):618-22. [PubMed: 10395649]  [MGI Ref ID J:56162]

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Woelbing F; Kostka SL; Moelle K; Belkaid Y; Sunderkoetter C; Verbeek S; Waisman A; Nigg AP; Knop J; Udey MC; von Stebut E. 2006. Uptake of Leishmania major by dendritic cells is mediated by Fcgamma receptors and facilitates acquisition of protective immunity. J Exp Med 203(1):177-88. [PubMed: 16418399]  [MGI Ref ID J:118805]

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Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Room Number           FGB29

Colony Maintenance

Mating System	Homozygote x Homozygote         (Female x Male)
Diet Information	LabDiet® 5K52/5K67

Purchasing information

Pricing, Supply Level & Notes, Controls, General Terms & Conditions

Pricing

Supply Details

Standard Supply

Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of ~nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within 48 hours of order placement.

Supply Notes

Usually shipped between four and eight weeks of age. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.

Control Information

	Control
	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls
USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains.
International - Control Pricing Information for Genetically Engineered Mutant Strains.

General Terms and Conditions

See Terms of Use

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Ordering and Purchasing Information

      Purchasing Information
      JAX^® Mice Orders
      Surgical Services

Contact Information
Orders & Technical Support
Tel: 800.422.6423 or 207.288.5845
Fax: 207.288.6150
Technical Support Email Form

Terms of Use

Terms of Use

General Terms and Conditions

For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries

Contracts Administration

phone:	207-288-6470
fax:	207-288-6655

JAX^® Mice & Services Conditions of Use

“Each recipient institution, including its employees and other researchers under its control (RECIPIENT), of mice or services using mice from The Jackson Laboratory (TJL) agrees that such mice, descendants of those mice derived by inbreeding or crossbreeding, including unmodified derivatives of those mice or their descendants (“MICE”) shall not be: (i) used for any purpose other than the internal research of the RECIPIENT, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services with respect to MICE. Acceptance of MICE from TJL shall be deemed agreement by RECIPIENT to these conditions, and departure from these conditions requires The Jackson Laboratory’s prior written authorization.”

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of MICE, products or services, The Jackson Laboratory will, at its option, provide credit or replacement for the MICE or product received or the services provided.

No Liability

In no event shall The Jackson Laboratory, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, products or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of The Jackson Laboratory, its agents or employees. In purchasing or receiving MICE, products or services from The Jackson Laboratory, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges The Jackson Laboratory from all such causes of action or damages, and further agrees to defend and indemnify The Jackson Laboratory from any costs or damages arising out of any third party claims.

MICE and biological materials are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to The Jackson Laboratory’s MICE, products and services. In addition, special terms and conditions of sale of certain MICE, products and services may be set forth separately in The Jackson Laboratory web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, products and services by The Jackson Laboratory, and by its licensees and distributors.

Acceptance of delivery of MICE, products or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on The Jackson Laboratory, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, products services by The Jackson Laboratory.