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Strain Name:

B6;129S4-Nfkbiatm1Bal/J

Stock Number:

002850

Availability:

Repository-Cryopreserved

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General Terms and Conditions

Genes & Alleles   Nfkbia;   Nfkbiatm1Bal;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Targeted Mutation
Specieslaboratory mouse
Donating Investigator David Baltimore,   California Institute of Technology
Generation?+N1pN1

Strain Description
Mice homozygous for the Nfkbiatm1Bal mutation are runted, and have granulocytosis as well as abnormalities in epithelial keratinization. Neonates die at around 8 days of age.

Strain Development
The Nfkbia gene was disrupted using an in frame insertion vector containing lacZ. The 129-derived J1 ES cell line was used.

Mammalian Phenotype Terms assigned by genotype

Nfkbiatm1Bal/Nfkbiatm1Bal

        involves: 129S4/SvJae * C57BL/6J
  • lethality-postnatal
  • postnatal lethality (MGI Ref ID J:30058)
    • death occurred around the 1st week of age
  • growth/size phenotype
  • decreased body size (MGI Ref ID J:30058)
    • although of normal size at birth, homozygotes became noticeably smaller than normal by 3-4 days of age
    • by 7 days of age homozygotes were severely runted (1/3 normal weight)
  • hematopoietic system phenotype
  • abnormal granulocyte differentiation (MGI Ref ID J:30058)
    • elevated numbers of granulocyte precursors in bone marrow and spleen as animals age
  • decreased double-positive T cell number (MGI Ref ID J:30058)
    • after 6 days
  • increased leukocyte cell number (MGI Ref ID J:30058)
    • 3 fold increase in peripheral WBC count - about 95% neutrophils
  • spleen atrophy (MGI Ref ID J:30058)
    • small size by day 2 with a worsening size reduction until death
  • thymus atrophy (MGI Ref ID J:30058)
    • atrophy in mice over 6 days of age
  • immune system phenotype
  • abnormal granulocyte differentiation (MGI Ref ID J:30058)
    • elevated numbers of granulocyte precursors in bone marrow and spleen as animals age
  • decreased double-positive T cell number (MGI Ref ID J:30058)
    • after 6 days
  • increased leukocyte cell number (MGI Ref ID J:30058)
    • 3 fold increase in peripheral WBC count - about 95% neutrophils
  • spleen atrophy (MGI Ref ID J:30058)
    • small size by day 2 with a worsening size reduction until death
  • thymus atrophy (MGI Ref ID J:30058)
    • atrophy in mice over 6 days of age
  • skin/coat/nails phenotype
  • abnormal basal cell layer morphology (MGI Ref ID J:30058)
    • poorly defined basal layer
  • abnormal skin condition (MGI Ref ID J:30058)
    • flaky skin (MGI Ref ID J:30058)
      • significant sloughing
    • scaly skin (MGI Ref ID J:30058)

Gene & Allele Details

Allele Symbol Nfkbiatm1Bal
Allele Name targeted mutation 1, David Baltimore
Common Name(s) IkappaBalpha-;
Mutation Made By David Baltimore,   California Institute of Technology
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Nfkbia, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Chromosome 12
Gene Common Name(s) AI462015; I(Kappa)B(alpha); IKBA; MAD-3; NFKBI; Nfkbi; RL/IF-1; expressed sequence AI462015; nuclear factor of kappa light chain gene enhancer in B-cells inhibitor;
Molecular Note The lacZ gene was inserted in frame 13 nucleotides downstream of the translational start site followed by a PGK-neomycin gene in reverse orientation thus replacing the remainder of the gene. Absence of protein product was established by Western blot analysis. [MGI Ref ID J:30058]

Control Information

  Allele   Control
 Nfkbiatm1Bal  Wild-type from the colony
 Nfkbiatm1Bal  101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Additional Web Information

JAX® NOTES, Summer 2005; 498. Toll-like Receptor JAX® Mice for Immunological Research.

Research Applications

This mouse can be used to support research in many areas including:

Nfkbiatm1Bal related

Dermatology Research
Skin and Hair Texture Defects

Developmental Biology Research
Growth Defects

Immunology and Inflammation Research
Immunodeficiency

References

Selected Reference(s)

Beg AA; Sha WC; Bronson RT; Baltimore D. 1995. Constitutive NF-kappa B activation, enhanced granulopoiesis, and neonatal lethality in I kappa B alpha-deficient mice. Genes Dev 9(22):2736-46. [PubMed: 7590249]  [MGI Ref ID J:30058]

Additional References

Price and Supply Information

Strain Name: B6;129S4-Nfkbiatm1Bal/J
Stock Number: 002850

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

LicensingSee General Terms and Conditions below  
Control InformationView Control Information in Strain Details.

General Terms and Conditions

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The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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