Strain Name:

B6;129S4-Jak3tm1Ljb/J

Stock Number:

002852

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Mice homozygous for Jak3tm1Ljb have blocked B cell development at pre-B resulting in a significant decrease in peripheral IgM+ B cells. Proliferative responses to mitogenic signals are defective and Il2 receptor signaling is blocked. The overall phenotype results in a severely immunocompromised mouse.

Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Former Names B6.129S4-Jak3tm1Ljb    (Changed: 15-DEC-04 )
Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. Leslie Berg,   University of Massachusetts Medical School

Appearance
black
Related Genotype: a/a

Description
Mice homozygous for the Jak3tm1Ljb mutation are viable and fertile. B cell development is blocked at pre-B resulting in a significant decrease in peripheral IgM+ B cells. The thymus is small but T cell development is relatively normal. There are increased numbers of CD4+ Cd8- cells in some homozygotes. Proliferative responses to mitogenic signals are defective and Il2 receptor signaling is blocked. The overall phenotype results in a severely immunocompromised mouse.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).
Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Positive
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-Negative, B Cell-Positive, Nk Cell-Negative   (JAK3)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Jak3tm1Ljb/Jak3tm1Ljb

        involves: 129S4/SvJae * C57BL/6
  • immune system phenotype
  • abnormal B cell morphology   (MGI Ref ID J:29722)
    • arrested B cell differentiation
      • profound block in B cell development at the pre-B cell stage   (MGI Ref ID J:29722)
      • however, total bone marrow cell numbers are similar to wild-type controls   (MGI Ref ID J:29722)
    • decreased immature B cell number
      • decreased numbers of CD43- CD45R+ cells are detected in the bone marrow or spleen   (MGI Ref ID J:29722)
      • however, no significant decrease in the number of CD43+ CD45R+ cells is detected   (MGI Ref ID J:29722)
    • decreased mature B cell number
      • virtually no CD45R+ IgM+ cells are detected in the bone marrow or spleen   (MGI Ref ID J:29722)
      • peritoneal lavage cells contain significantly fewer CD45R+ IgM+ cells   (MGI Ref ID J:29722)
  • abnormal immune system physiology   (MGI Ref ID J:29722)
    • abnormal leukocyte physiology
      • decrease in the chemotactic response of thymocytes towards CCL25 and CXCL12   (MGI Ref ID J:90510)
      • however, chemotactic responses towards CCL5, CCL11 and CCL14 are not significantly different from heterozygous controls   (MGI Ref ID J:90510)
      • abnormal lymphocyte physiology
        • decrease in the chemotactic response of bone marrow cells towards CCL25 and CXCL12   (MGI Ref ID J:90510)
        • cells that do migrate towards CCL25 and CXCL12 are enriched for hematopoietic progenitors   (MGI Ref ID J:90510)
        • abnormal T cell activation
          • splenic T cells stimulated with anti-CD3 plus anti-CD28 secrete significantly less IL-2   (MGI Ref ID J:29722)
          • most of the splenic T cells express markers of activation and are larger than wild-type splenic T cells   (MGI Ref ID J:29722)
          • decreased T cell proliferation
            • mitogen-induced thymocyte and splenic T cell proliferation responses are significantly reduced compared to wild-type cells   (MGI Ref ID J:29722)
        • decreased IgG level
          • serum IgG levels appear to be lower in mice at 3 months of age   (MGI Ref ID J:29722)
          • however, in younger mice serum Ig levels are similar to wild-type   (MGI Ref ID J:29722)
    • abnormal response to infection
      • the LPS-induced proliferation response of spleen cells is significantly reduced   (MGI Ref ID J:29722)
  • abnormal splenic cell ratio
    • spleens contain an increased percentage of Thy1- CD45R- Mac1- cells   (MGI Ref ID J:29722)
  • small Peyer's patches
    • Peyer's patches are almost undetectable   (MGI Ref ID J:29722)
  • small lymph nodes
    • peripheral lymph nodes are nearly undetectable   (MGI Ref ID J:29722)
    • lymph node hypoplasia
      • peripheral lymph nodes contain only about 2% the normal number of cells   (MGI Ref ID J:29722)
      • the ratio of CD4+ to CD8+ cells is increase with more than 60% of the cells being CD4+ and less than 5% being CD8+   (MGI Ref ID J:29722)
  • small thymus   (MGI Ref ID J:29722)
    • thymus hypoplasia
      • total cell numbers range from 0.5 to 10% of wild-type controls   (MGI Ref ID J:29722)
      • however, the proportion of different cell types is similar to controls   (MGI Ref ID J:29722)
  • spleen hypoplasia
    • total spleen cell numbers range from 10 to 500% of wild-type controls   (MGI Ref ID J:29722)
  • hematopoietic system phenotype
  • abnormal B cell morphology   (MGI Ref ID J:29722)
    • arrested B cell differentiation
      • profound block in B cell development at the pre-B cell stage   (MGI Ref ID J:29722)
      • however, total bone marrow cell numbers are similar to wild-type controls   (MGI Ref ID J:29722)
    • decreased immature B cell number
      • decreased numbers of CD43- CD45R+ cells are detected in the bone marrow or spleen   (MGI Ref ID J:29722)
      • however, no significant decrease in the number of CD43+ CD45R+ cells is detected   (MGI Ref ID J:29722)
    • decreased mature B cell number
      • virtually no CD45R+ IgM+ cells are detected in the bone marrow or spleen   (MGI Ref ID J:29722)
      • peritoneal lavage cells contain significantly fewer CD45R+ IgM+ cells   (MGI Ref ID J:29722)
  • abnormal leukocyte physiology
    • decrease in the chemotactic response of thymocytes towards CCL25 and CXCL12   (MGI Ref ID J:90510)
    • however, chemotactic responses towards CCL5, CCL11 and CCL14 are not significantly different from heterozygous controls   (MGI Ref ID J:90510)
    • abnormal lymphocyte physiology
      • decrease in the chemotactic response of bone marrow cells towards CCL25 and CXCL12   (MGI Ref ID J:90510)
      • cells that do migrate towards CCL25 and CXCL12 are enriched for hematopoietic progenitors   (MGI Ref ID J:90510)
      • abnormal T cell activation
        • splenic T cells stimulated with anti-CD3 plus anti-CD28 secrete significantly less IL-2   (MGI Ref ID J:29722)
        • most of the splenic T cells express markers of activation and are larger than wild-type splenic T cells   (MGI Ref ID J:29722)
        • decreased T cell proliferation
          • mitogen-induced thymocyte and splenic T cell proliferation responses are significantly reduced compared to wild-type cells   (MGI Ref ID J:29722)
      • decreased IgG level
        • serum IgG levels appear to be lower in mice at 3 months of age   (MGI Ref ID J:29722)
        • however, in younger mice serum Ig levels are similar to wild-type   (MGI Ref ID J:29722)
  • abnormal splenic cell ratio
    • spleens contain an increased percentage of Thy1- CD45R- Mac1- cells   (MGI Ref ID J:29722)
  • small thymus   (MGI Ref ID J:29722)
    • thymus hypoplasia
      • total cell numbers range from 0.5 to 10% of wild-type controls   (MGI Ref ID J:29722)
      • however, the proportion of different cell types is similar to controls   (MGI Ref ID J:29722)
  • spleen hypoplasia
    • total spleen cell numbers range from 10 to 500% of wild-type controls   (MGI Ref ID J:29722)
  • endocrine/exocrine gland phenotype
  • small thymus   (MGI Ref ID J:29722)
    • thymus hypoplasia
      • total cell numbers range from 0.5 to 10% of wild-type controls   (MGI Ref ID J:29722)
      • however, the proportion of different cell types is similar to controls   (MGI Ref ID J:29722)

Jak3tm1Ljb/Jak3tm1Ljb

        B6;129S4-Jak3tm1Ljb/J
  • immune system phenotype
  • abnormal dendritic cell morphology   (MGI Ref ID J:123902)
    • abnormal dendritic cell differentiation
      • splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls   (MGI Ref ID J:123902)
      • however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls   (MGI Ref ID J:123902)
    • decreased myeloid dendritic cell number
      • the proportion and absolute number of CD11c+ dendritic cells is decreased in the spleen   (MGI Ref ID J:123902)
      • however, the frequency plasmacytoid dendritic cells in the spleen is not significantly different from wild-type controls   (MGI Ref ID J:123902)
  • abnormal dendritic cell physiology
    • in culture, dendritic cells are resistant to cytokine withdrawal-induced apoptosis   (MGI Ref ID J:123902)
    • bone marrow-derived dendritic cells overproduce IL12 and IL10   (MGI Ref ID J:123902)
    • when bone marrow-derived dendritic cells are used in an adoptive transfer model the proportion of IFNG-producing cells is increased compared to when wild-type cells are used   (MGI Ref ID J:123902)
  • abnormal splenic cell ratio
    • reduced numbers of splenocytes   (MGI Ref ID J:123902)
    • decrease in the proportion and absolute number of CD11c+ dendritic, CD4+, and CD8+ cells in the spleen   (MGI Ref ID J:123902)
    • however, the frequency plasmacytoid dendritic cells is not significantly different from wild-type controls   (MGI Ref ID J:123902)
  • decreased CD4-positive, alpha beta T cell number
    • decrease in the proportion and absolute number of CD4+ cells in the spleen   (MGI Ref ID J:123902)
  • decreased CD8-positive, alpha-beta T cell number
    • decrease in the proportion and absolute number of CD8+ cells in the spleen   (MGI Ref ID J:123902)
  • hematopoietic system phenotype
  • abnormal dendritic cell morphology   (MGI Ref ID J:123902)
    • abnormal dendritic cell differentiation
      • splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls   (MGI Ref ID J:123902)
      • however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls   (MGI Ref ID J:123902)
    • decreased myeloid dendritic cell number
      • the proportion and absolute number of CD11c+ dendritic cells is decreased in the spleen   (MGI Ref ID J:123902)
      • however, the frequency plasmacytoid dendritic cells in the spleen is not significantly different from wild-type controls   (MGI Ref ID J:123902)
  • abnormal splenic cell ratio
    • reduced numbers of splenocytes   (MGI Ref ID J:123902)
    • decrease in the proportion and absolute number of CD11c+ dendritic, CD4+, and CD8+ cells in the spleen   (MGI Ref ID J:123902)
    • however, the frequency plasmacytoid dendritic cells is not significantly different from wild-type controls   (MGI Ref ID J:123902)
  • decreased CD4-positive, alpha beta T cell number
    • decrease in the proportion and absolute number of CD4+ cells in the spleen   (MGI Ref ID J:123902)
  • decreased CD8-positive, alpha-beta T cell number
    • decrease in the proportion and absolute number of CD8+ cells in the spleen   (MGI Ref ID J:123902)
  • cellular phenotype
  • abnormal dendritic cell differentiation
    • splenic dendritic cells express higher levels of activation markers compared to cells from wild-type controls   (MGI Ref ID J:123902)
    • however, bone marrow precursors in vitro show a similar capacity to generate immature dendritic cells compared to wild-type controls   (MGI Ref ID J:123902)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Jak3tm1Ljb/Jak3+

        involves: 129S4/SvJae
  • immune system phenotype
  • abnormal leukocyte morphology
    • slight increase in the number of cells classified as myeloid or premonocytic   (MGI Ref ID J:56629)
    • decreased lymphocyte cell number
      • lymphopenia is seen in peripheral and bone marrow smears   (MGI Ref ID J:56629)
      • at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased   (MGI Ref ID J:56629)
    • increased leukocyte cell number
      • at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen   (MGI Ref ID J:56629)
      • these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage   (MGI Ref ID J:56629)
      • increased monocyte cell number
        • at 6 - 12 months of age in the bone marrow and spleen   (MGI Ref ID J:56629)
      • increased neutrophil cell number
        • significantly higher numbers of both segmented and band neutrophils   (MGI Ref ID J:56629)
  • hematopoietic system phenotype
  • abnormal leukocyte morphology
    • slight increase in the number of cells classified as myeloid or premonocytic   (MGI Ref ID J:56629)
    • decreased lymphocyte cell number
      • lymphopenia is seen in peripheral and bone marrow smears   (MGI Ref ID J:56629)
      • at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased   (MGI Ref ID J:56629)
    • increased leukocyte cell number
      • at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen   (MGI Ref ID J:56629)
      • these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage   (MGI Ref ID J:56629)
      • increased monocyte cell number
        • at 6 - 12 months of age in the bone marrow and spleen   (MGI Ref ID J:56629)
      • increased neutrophil cell number
        • significantly higher numbers of both segmented and band neutrophils   (MGI Ref ID J:56629)

Jak3tm1Ljb/Jak3tm1Ljb

        involves: 129S4/SvJae
  • immune system phenotype
  • abnormal T cell physiology
    • thymocytes produce less IL12 and IL13 in response to anti-CD3 plus anti-CD28-stimulation compared to wild-type controls   (MGI Ref ID J:128694)
    • splenic T cells secrete less IL12 in response to T cell receptor plus CD28 stimulation   (MGI Ref ID J:128694)
  • abnormal leukocyte morphology
    • large increase in the number of cells classified as myeloid or premonocytic   (MGI Ref ID J:56629)
    • abnormal T cell differentiation
      • the proportion of pro-T cells is increased about 3-fold and fewer cells in transition between pre-T and late pre-T cell stages are found   (MGI Ref ID J:64861)
      • both pro-T and pre-T cells have increased CD25 expression   (MGI Ref ID J:64861)
      • about a 2-fold increase in apoptotic thymocytes is seen from E14 - E17   (MGI Ref ID J:64861)
      • intrathymically injected bone marrow cells show extremely limited ability to reconstitute T cell development especially when in competition with heterozygous or wild-type cells   (MGI Ref ID J:64861)
      • most intrathymically injected cells arrest at the CD44+ CD25- double negativeprogenitor cell stage   (MGI Ref ID J:64861)
      • from E15 - E17, T cell maturation appears to be delayed by about 1 day compared to age-matched heterozygous littermates   (MGI Ref ID J:64861)
      • however, by E18 T cell maturation in homozygous mice has caught up to that of their heterozygous littermates   (MGI Ref ID J:64861)
      • abnormal T cell activation
        • CD4+ cells that are present show an activated phenotype   (MGI Ref ID J:56629)
        • CD4+ T cells resemble activated or memory T cells   (MGI Ref ID J:128694)
        • decreased T cell proliferation
          • decrease in thymocyte proliferation in response to CD3 plus CD28 stimulation compared to wild-type   (MGI Ref ID J:128694)
          • the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is virtually absent   (MGI Ref ID J:128694)
    • decreased lymphocyte cell number
      • lymphopenia is seen in peripheral and bone marrow smears   (MGI Ref ID J:56629)
      • at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased   (MGI Ref ID J:56629)
      • absent B cells   (MGI Ref ID J:128694)
      • decreased thymocyte number
        • in adult mice, there is about a 20-fold reduction in thymocyte numbers   (MGI Ref ID J:64861)
        • at E14 - E15, thymocytes are virtually undetectable   (MGI Ref ID J:64861)
        • at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates   (MGI Ref ID J:64861)
        • despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates   (MGI Ref ID J:64861)
    • increased leukocyte cell number
      • at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen   (MGI Ref ID J:56629)
      • these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage   (MGI Ref ID J:56629)
      • increased monocyte cell number
        • at 6 - 12 months of age in the bone marrow and spleen   (MGI Ref ID J:56629)
      • increased neutrophil cell number
        • significantly higher numbers of both segmented and band neutrophils   (MGI Ref ID J:56629)
  • abnormal myelopoiesis
    • increase in the number of myeloid progenitor cells in the peripheral blood and bone marrow   (MGI Ref ID J:56629)
  • abnormal spleen morphology
    • the general architecture of the spleen is severely disrupted   (MGI Ref ID J:56629)
    • abnormal spleen white pulp morphology
      • the white pulp is replaced with a collection of large cells with euchromatic nuclei that are CD3+   (MGI Ref ID J:56629)
      • numerous megakaryoctes are present   (MGI Ref ID J:56629)
    • enlarged spleen
      • may be seen in some mice by as early as 4 months of age   (MGI Ref ID J:56629)
      • seen in all mice over 5 months of age   (MGI Ref ID J:56629)
  • abnormal thymus cell ratio
    • increase in the ratio of CD4+ to CD8+ cells   (MGI Ref ID J:128694)
  • absent lymph nodes   (MGI Ref ID J:128694)
  • chronic inflammation
    • widespread infiltration of the lungs, kidneys, and liver with mononuclear cells   (MGI Ref ID J:56629)
  • thymus hypoplasia   (MGI Ref ID J:128694)
  • hematopoietic system phenotype
  • abnormal T cell physiology
    • thymocytes produce less IL12 and IL13 in response to anti-CD3 plus anti-CD28-stimulation compared to wild-type controls   (MGI Ref ID J:128694)
    • splenic T cells secrete less IL12 in response to T cell receptor plus CD28 stimulation   (MGI Ref ID J:128694)
  • abnormal erythropoiesis
    • slight decrease in the number of erythroid progenitors in the bone marrow   (MGI Ref ID J:56629)
  • abnormal leukocyte morphology
    • large increase in the number of cells classified as myeloid or premonocytic   (MGI Ref ID J:56629)
    • abnormal T cell differentiation
      • the proportion of pro-T cells is increased about 3-fold and fewer cells in transition between pre-T and late pre-T cell stages are found   (MGI Ref ID J:64861)
      • both pro-T and pre-T cells have increased CD25 expression   (MGI Ref ID J:64861)
      • about a 2-fold increase in apoptotic thymocytes is seen from E14 - E17   (MGI Ref ID J:64861)
      • intrathymically injected bone marrow cells show extremely limited ability to reconstitute T cell development especially when in competition with heterozygous or wild-type cells   (MGI Ref ID J:64861)
      • most intrathymically injected cells arrest at the CD44+ CD25- double negativeprogenitor cell stage   (MGI Ref ID J:64861)
      • from E15 - E17, T cell maturation appears to be delayed by about 1 day compared to age-matched heterozygous littermates   (MGI Ref ID J:64861)
      • however, by E18 T cell maturation in homozygous mice has caught up to that of their heterozygous littermates   (MGI Ref ID J:64861)
      • abnormal T cell activation
        • CD4+ cells that are present show an activated phenotype   (MGI Ref ID J:56629)
        • CD4+ T cells resemble activated or memory T cells   (MGI Ref ID J:128694)
        • decreased T cell proliferation
          • decrease in thymocyte proliferation in response to CD3 plus CD28 stimulation compared to wild-type   (MGI Ref ID J:128694)
          • the proliferative response of splenic T cells in response to T cell receptor plus CD28 stimulation is virtually absent   (MGI Ref ID J:128694)
    • decreased lymphocyte cell number
      • lymphopenia is seen in peripheral and bone marrow smears   (MGI Ref ID J:56629)
      • at 6-12 months of age, lymphocyte numbers in whole blood suspensions are decreased   (MGI Ref ID J:56629)
      • absent B cells   (MGI Ref ID J:128694)
      • decreased thymocyte number
        • in adult mice, there is about a 20-fold reduction in thymocyte numbers   (MGI Ref ID J:64861)
        • at E14 - E15, thymocytes are virtually undetectable   (MGI Ref ID J:64861)
        • at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates   (MGI Ref ID J:64861)
        • despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates   (MGI Ref ID J:64861)
    • increased leukocyte cell number
      • at 6-12 months of age, a profound increase in large granular cells is seen in the peripheral blood and spleen   (MGI Ref ID J:56629)
      • these large granular cells consist of 2 populations; cells of the neutrophilic lineage and cells of the monocytic lineage   (MGI Ref ID J:56629)
      • increased monocyte cell number
        • at 6 - 12 months of age in the bone marrow and spleen   (MGI Ref ID J:56629)
      • increased neutrophil cell number
        • significantly higher numbers of both segmented and band neutrophils   (MGI Ref ID J:56629)
  • abnormal myelopoiesis
    • increase in the number of myeloid progenitor cells in the peripheral blood and bone marrow   (MGI Ref ID J:56629)
  • abnormal spleen morphology
    • the general architecture of the spleen is severely disrupted   (MGI Ref ID J:56629)
    • abnormal spleen white pulp morphology
      • the white pulp is replaced with a collection of large cells with euchromatic nuclei that are CD3+   (MGI Ref ID J:56629)
      • numerous megakaryoctes are present   (MGI Ref ID J:56629)
    • enlarged spleen
      • may be seen in some mice by as early as 4 months of age   (MGI Ref ID J:56629)
      • seen in all mice over 5 months of age   (MGI Ref ID J:56629)
  • abnormal thymus cell ratio
    • increase in the ratio of CD4+ to CD8+ cells   (MGI Ref ID J:128694)
  • thymus hypoplasia   (MGI Ref ID J:128694)
  • endocrine/exocrine gland phenotype
  • abnormal thymus cell ratio
    • increase in the ratio of CD4+ to CD8+ cells   (MGI Ref ID J:128694)
  • decreased thymocyte number
    • in adult mice, there is about a 20-fold reduction in thymocyte numbers   (MGI Ref ID J:64861)
    • at E14 - E15, thymocytes are virtually undetectable   (MGI Ref ID J:64861)
    • at E14 only about 200 Thy+ cells are present in the thymus compared to around 20,000 in age-matched heterozygous littermates   (MGI Ref ID J:64861)
    • despite the deficit in progenitors, the rate of thymocyte expansion between E14 and E18 and differentiation of thymic progenitors into pro-T cells are similar to that of heterozygous littermates   (MGI Ref ID J:64861)
  • thymus hypoplasia   (MGI Ref ID J:128694)
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Research Applications
This mouse can be used to support research in many areas including:

Jak3tm1Ljb related

Immunology, Inflammation and Autoimmunity Research
Immunodeficiency
      B, T, and NK cell deficiency
Intracellular Signaling Molecules

Research Tools
Cancer Research
      B, T, and NK cell deficiency, xenograft/transplant host
Immunology, Inflammation and Autoimmunity Research
      B cell deficiency

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Jak3tm1Ljb
Allele Name targeted mutation 1, Leslie J Berg
Allele Type Targeted (Null/Knockout)
Common Name(s) Jak-3-; Jak3-;
Mutation Made ByDr. Leslie Berg,   University of Massachusetts Medical School
Strain of Origin129S4/SvJae
ES Cell Line NameJ1
ES Cell Line Strain129S4/SvJae
Gene Symbol and Name Jak3, Janus kinase 3
Chromosome 8
Gene Common Name(s) JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK; RATJAK3; wil; willow;
Molecular Note A neomycin resistance cassette replaced a 0.6 kb fragment of the gene, which contains sequences encoding subdomains I to IV of the kinase domain. Protein immunoblots of lysates from bone marrow, spleen, and thymus showed no targeted protein. [MGI Ref ID J:29722]

Genotyping

Genotyping Information

Genotyping Protocols

Jak3tm1Ljb, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Thomis DC; Gurniak CB; Tivol E; Sharpe AH; Berg LJ. 1995. Defects in B lymphocyte maturation and T lymphocyte activation in mice lacking Jak3. Science 270(5237):794-7. [PubMed: 7481767]  [MGI Ref ID J:29722]

Additional References

Antov A; Yang L; Vig M; Baltimore D; Van Parijs L. 2003. Essential role for STAT5 signaling in CD25+CD4+ regulatory T cell homeostasis and the maintenance of self-tolerance. J Immunol 171(7):3435-41. [PubMed: 14500638]  [MGI Ref ID J:85631]

Soldevila G; Licona I; Salgado A; Ramirez M; Chavez R; Garcia-Zepeda E. 2004. Impaired chemokine-induced migration during T-cell development in the absence of Jak 3. Immunology 112(2):191-200. [PubMed: 15147562]  [MGI Ref ID J:90510]

Jak3tm1Ljb related

Baird AM; Lucas JA; Berg LJ. 2000. A profound deficiency in thymic progenitor cells in mice lacking Jak3 J Immunol 165(7):3680-8. [PubMed: 11034372]  [MGI Ref ID J:64861]

Bell BD; Kitajima M; Larson RP; Stoklasek TA; Dang K; Sakamoto K; Wagner KU; Reizis B; Hennighausen L; Ziegler SF. 2013. The transcription factor STAT5 is critical in dendritic cells for the development of TH2 but not TH1 responses. Nat Immunol 14(4):364-71. [PubMed: 23435120]  [MGI Ref ID J:194747]

Bongfen SE; Rodrigue-Gervais IG; Berghout J; Torre S; Cingolani P; Wiltshire SA; Leiva-Torres GA; Letourneau L; Sladek R; Blanchette M; Lathrop M; Behr MA; Gruenheid S; Vidal SM; Saleh M; Gros P. 2012. An N-ethyl-N-nitrosourea (ENU)-induced dominant negative mutation in the JAK3 kinase protects against cerebral malaria. PLoS One 7(2):e31012. [PubMed: 22363534]  [MGI Ref ID J:185217]

Cho JH; Kim HO; Kim KS; Yang DH; Surh CD; Sprent J. 2013. Unique features of naive CD8+ T cell activation by IL-2. J Immunol 191(11):5559-73. [PubMed: 24166977]  [MGI Ref ID J:207012]

Dillon SR; Schlissel MS. 2002. Partial restoration of B cell development in Jak-3(-/-) mice achieved by co-expression of IgH and E(mu)-myc transgenes. Int Immunol 14(8):893-904. [PubMed: 12147626]  [MGI Ref ID J:78457]

Garcia-Zepeda EA; Licona-Limon I; Jimenez-Solomon MF; Soldevila G. 2007. Janus kinase 3-deficient T lymphocytes have an intrinsic defect in CCR7-mediated homing to peripheral lymphoid organs. Immunology 122(2):247-60. [PubMed: 17521370]  [MGI Ref ID J:125641]

Gozalo-Sanmillan S; McNally JM; Lin MY; Chambers CA; Berg LJ. 2001. Cutting edge: two distinct mechanisms lead to impaired T cell homeostasis in Janus kinase 3- and CTLA-4-deficient mice. J Immunol 166(2):727-30. [PubMed: 11145642]  [MGI Ref ID J:127073]

Grossman WJ; Verbsky JW; Yang L; Berg LJ; Fields LE; Chaplin DD; Ratner L. 1999. Dysregulated myelopoiesis in mice lacking Jak3. Blood 94(3):932-9. [PubMed: 10419884]  [MGI Ref ID J:56629]

Junttila IS; Mizukami K; Dickensheets H; Meier-Schellersheim M; Yamane H; Donnelly RP; Paul WE. 2008. Tuning sensitivity to IL-4 and IL-13: differential expression of IL-4Ralpha, IL-13Ralpha1, and gammac regulates relative cytokine sensitivity. J Exp Med 205(11):2595-608. [PubMed: 18852293]  [MGI Ref ID J:141103]

Lange C; Thiersch M; Samardzija M; Burgi S; Joly S; Grimm C. 2010. LIF-dependent JAK3 activation is not essential for retinal degeneration. J Neurochem 113(5):1210-20. [PubMed: 20345762]  [MGI Ref ID J:161419]

Li L; Xia Y; Nguyen A; Lai YH; Feng L; Mosmann TR; Lo D. 1999. Effects of Th2 cytokines on chemokine expression in the lung: IL-13 potently induces eotaxin expression by airway epithelial cells. J Immunol 162(5):2477-87. [PubMed: 10072486]  [MGI Ref ID J:112256]

Lv N; Kim EK; Song MY; Choi HN; Moon WS; Park SJ; Park JW; Kwon KB; Park BH. 2009. JANEX-1, a JAK3 inhibitor, protects pancreatic islets from cytokine toxicity through downregulation of NF-kappaB activation and the JAK/STAT pathway. Exp Cell Res 315(12):2064-71. [PubMed: 19414010]  [MGI Ref ID J:150450]

Mayack SR; Berg LJ. 2006. Cutting edge: an alternative pathway of CD4+ T cell differentiation is induced following activation in the absence of gamma-chain-dependent cytokine signals. J Immunol 176(4):2059-63. [PubMed: 16455959]  [MGI Ref ID J:129213]

Mishra J; Verma RK; Alpini G; Meng F; Kumar N. 2013. Role of Janus kinase 3 in mucosal differentiation and predisposition to colitis. J Biol Chem 288(44):31795-806. [PubMed: 24045942]  [MGI Ref ID J:204900]

Rivas-Caicedo A; Soldevila G; Fortoul TI; Castell-Rodriguez A; Flores-Romo L; Garcia-Zepeda EA. 2009. Jak3 is involved in dendritic cell maturation and CCR7-dependent migration. PLoS One 4(9):e7066. [PubMed: 19759904]  [MGI Ref ID J:153621]

Rochman Y; Kashyap M; Robinson GW; Sakamoto K; Gomez-Rodriguez J; Wagner KU; Leonard WJ. 2010. Thymic stromal lymphopoietin-mediated STAT5 phosphorylation via kinases JAK1 and JAK2 reveals a key difference from IL-7-induced signaling. Proc Natl Acad Sci U S A 107(45):19455-60. [PubMed: 20974963]  [MGI Ref ID J:167374]

Schulteis RD; Chu H; Dai X; Chen Y; Edwards B; Haribhai D; Williams CB; Malarkannan S; Hessner MJ; Glisic-Milosavljevic S; Jana S; Kerschen EJ; Ghosh S; Wang D; Kwitek AE; Lernmark A; Gorski J; Weiler H. 2008. Impaired survival of peripheral T cells, disrupted NK/NKT cell development, and liver failure in mice lacking Gimap5. Blood 112(13):4905-14. [PubMed: 18796632]  [MGI Ref ID J:143620]

Shi M; Lin TH; Appell KC; Berg LJ. 2009. Cell cycle progression following naive T cell activation is independent of Jak3/common gamma-chain cytokine signals. J Immunol 183(7):4493-501. [PubMed: 19734221]  [MGI Ref ID J:152788]

Shi M; Lin TH; Appell KC; Berg LJ. 2008. Janus-kinase-3-dependent signals induce chromatin remodeling at the Ifng locus during T helper 1 cell differentiation. Immunity 28(6):763-73. [PubMed: 18549798]  [MGI Ref ID J:137735]

Soldevila G; Licona I; Salgado A; Ramirez M; Chavez R; Garcia-Zepeda E. 2004. Impaired chemokine-induced migration during T-cell development in the absence of Jak 3. Immunology 112(2):191-200. [PubMed: 15147562]  [MGI Ref ID J:90510]

Thomis DC; Berg LJ. 1997. Peripheral expression of Jak3 is required to maintain T lymphocyte function. J Exp Med 185(2):197-206. [PubMed: 9016869]  [MGI Ref ID J:128694]

Thomis DC; Lee W; Berg LJ. 1997. T cells from Jak3-deficient mice have intact TCR signaling, but increased apoptosis. J Immunol 159(10):4708-19. [PubMed: 9366394]  [MGI Ref ID J:43961]

Verbsky JW; Randolph DA; Shornick LP; Chaplin DD. 2002. Nonhematopoietic Expression of Janus Kinase 3 Is Required for Efficient Recruitment of Th2 Lymphocytes and Eosinophils in OVA-Induced Airway Inflammation. J Immunol 168(5):2475-82. [PubMed: 11859141]  [MGI Ref ID J:74723]

Wang H; Brown J; Gao S; Liang S; Jotwani R; Zhou H; Suttles J; Scott DA; Lamont RJ. 2013. The role of JAK-3 in regulating TLR-mediated inflammatory cytokine production in innate immune cells. J Immunol 191(3):1164-74. [PubMed: 23797672]  [MGI Ref ID J:205730]

Yamaoka K; Min B; Zhou YJ; Paul WE; O'shea JJ. 2005. Jak3 negatively regulates dendritic-cell cytokine production and survival. Blood 106(9):3227-33. [PubMed: 16020505]  [MGI Ref ID J:123902]

Health & husbandry

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Colony Maintenance

Breeding & HusbandryColony is currently maintained homozygote x homozygote. Expected coat color from breeding is black or agouti.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2525.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $1650.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3283.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Frozen Products

Price (US dollars $)
Frozen Embryo $2145.00

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryopreserved Embryos
    Available to most shipping destinations1
    This strain is also available as cryopreserved embryos2. Orders for cryopreserved embryos may be placed with our Customer Service Department. Experienced technicians at The Jackson Laboratory have recovered frozen embryos of this strain successfully. We will provide you enough embryos to perform two embryo transfers. The Jackson Laboratory does not guarantee successful recovery at your facility. For complete information on purchasing embryos, please visit our Cryopreserved Embryos web page.

    1 Shipments cannot be made to Australia due to Australian government import restrictions.
    2 Embryos for most strains are cryopreserved at the two cell stage while some strains are cryopreserved at the eight cell stage. If this information is important to you, please contact Customer Service.
  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   101045 B6129SF2/J (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.


See Terms of Use tab for General Terms and Conditions


The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
Ordering Information
JAX® Mice
Surgical and Preconditioning Services
JAX® Services
Customer Services and Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form

Terms of Use

Terms of Use


General Terms and Conditions


Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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