| |||||||||||
- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
Strain Information
Former Names FVB/N-TgN(TIE2LacZ)182Sato (Changed: 15-DEC-04 ) Type Mutant Strain; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Species laboratory mouse Generation N?+28 (13-NOV-09) Donating Investigator Thomas Sato, Weill Medical College of Cornell Univ Appearance
albino
Related Genotype: Tyrc/TyrcImportant Note
This strain is homozygous for the retinal degeneration allele Pde6brd1.Description
Transgenic mice carry a beta-galactosidase reporter gene under the control of the murine Tek (Tie2) promoter. LacZ is expressed specifically in vascular endothelial cells in embryonic and adult mice. The transgenic line may be useful when crossed with tumor producing strains and the transgene used to visualize neovascularization during tumorigenesis.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
Related Strains
lacZ Expression Strains
View lacZ Expression Strains (186 strains)
005707 STOCK Rag1tm1Mom Tg(TIE2-lacZ)182Sato/J
004297 B6.CXB1-Pde6brd10/J 005252 B6EiC3Sn.BLiA-Ts(1716)65Dn/DnJ 003647 B6EiC3Sn.BLiAF1 002802 C3.BLiA Pde6b+-Krd/J 001979 C3A.BLiA-Pde6b+.O20-Prph2Rd2/J 001912 C3A.BLiA-Pde6b+/J 003648 C3Sn.BLiA-Pde6b+/Dn 004766 C57BL/6J-Pde6brd1-2J/J 004828 FVB.129P2-Pde6b+ Tyrc-ch/AntJ 004808 STOCK Mapttm1(EGFP)Klt Tg(MAPT)8cPdav/J
004128 B6.Cg-Tg(Tek-cre)12Flv/J 008863 B6.Cg-Tg(Tek-cre)1Ywa/J 008537 FVB-Tg(Tek-cre)2352Rwng/J 006770 STOCK Rag1tm1Mom Tg(TIE2GFP)287Sato/J 003658 STOCK Tg(TIE2GFP)287Sato/J
Additional Web Information
Fluorescent Proteins/lacZ Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
lacZ relatedResearch Tools
lacZ Expression
Cancer Research
endothelial cell marker for neovascularization
xenograft/transplant host
Cardiovascular Research
endothelial cell marker (lacZ) for neovascularization
endothelial cell marker for neovascularization
Developmental Biology Research
Genetics Research
Tissue/Cell Markers: endothelial cell markers for neovascularization
Pde6brd1 relatedResearch Tools
lacZ Expression
Mouse/Human Gene Homologs
retinitis pigmentosa, autosomal recessive
Sensorineural Research
Retinal Degeneration
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(TIE2-lacZ)182Sato | ||
|---|---|---|---|
| Allele Name | transgene insertion 182, Thomas N Sato | ||
| Allele Type | Transgenic (Reporter) | ||
| Common Name(s) | TIE2p/e-LacZ; Tg(TIE2LacZ)182Sato; Tg182Sato; Tie2-lacZ; Tie2/LacZ; | ||
| Mutation Made By | Thomas Sato, Weill Medical College of Cornell Univ | ||
| Site of Expression | lacZ expression is detected in endothelial cells. | ||
| Expressed Gene | lacZ, beta-galactosidase, E. coli | ||
| Promoter | Tek, endothelial-specific receptor tyrosine kinase, mouse, laboratory | ||
| General Note | In embryonic and adult transgenic mice on an FVB/N background, LacZ is expressed specifically in vascular endothelial cells. | ||
| Molecular Note | The transgene consists of a mouse Tek promoter and enhancer element, a lacZ reporter gene, and an SV40 polyadenylation sequence. The enhancer element is a genomic fragment extending from 3' of exon 1 to 10 kb into intron 1. Expression was driven in endothelial cells. [MGI Ref ID J:39276] | ||
| Gene Symbol and Name | Tg(TIE2-lacZ)182Sato, transgene insertion 182, Thomas N Sato | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(TIE2LacZ)182Sato; Tg182Sato; Tie2-lacZ; Tie2/LacZ; | ||
| Allele Symbol | Pde6brd1 | ||
| Allele Name | retinal degeneration 1 | ||
| Allele Type | Spontaneous | ||
| Common Name(s) | Pdebrd1; rd; rd-1; rd1; rodless retina; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic LacZ Melt Curve Analysis, Melt Curve Analysis
Generic LacZ, Standard PCR
Helpful Links
Genotyping resources and troubleshooting
References
References
Selected Reference(s)
Schlaeger TM; Bartunkova S; Lawitts JA; Teichmann G; Risau W ; Deutsch U ; Sato TN. 1997. Uniform vascular-endothelial-cell-specific gene expression in both embryonic and adult transgenic mice. Proc Natl Acad Sci U S A 94(7):3058-63. [PubMed: 9096345] [MGI Ref ID J:39276]
Additional References
Oshima RG; Lesperance J; Munoz V; Hebbard L; Ranscht B; Sharan N; Muller WJ; Hauser CA; Cardiff RD. 2004. Angiogenic acceleration of Neu induced mammary tumor progression and metastasis. Cancer Res 64(1):169-79. [PubMed: 14729621] [MGI Ref ID J:87480]
Tg(TIE2-lacZ)182Sato relatedAdams RH; Diella F; Hennig S; Helmbacher F; Deutsch U; Klein R. 2001. The cytoplasmic domain of the ligand ephrinB2 is required for vascular morphogenesis but not cranial neural crest migration. Cell 104(1):57-69. [PubMed: 11163240] [MGI Ref ID J:67296]
Ahn GO; Brown JM. 2008. Matrix metalloproteinase-9 is required for tumor vasculogenesis but not for angiogenesis: role of bone marrow-derived myelomonocytic cells. Cancer Cell 13(3):193-205. [PubMed: 18328424] [MGI Ref ID J:132946]
Anghelina M; Krishnan P; Moldovan L; Moldovan NI. 2006. Monocytes/macrophages cooperate with progenitor cells during neovascularization and tissue repair: conversion of cell columns into fibrovascular bundles. Am J Pathol 168(2):529-41. [PubMed: 16436667] [MGI Ref ID J:106289]
Bushdid PB; Osinska H; Waclaw RR; Molkentin JD; Yutzey KE. 2003. NFATc3 and NFATc4 are required for cardiac development and mitochondrial function. Circ Res 92(12):1305-13. [PubMed: 12750314] [MGI Ref ID J:84138]
Clauss SB; Walker DL; Kirby ML; Schimel D; Lo CW. 2006. Patterning of coronary arteries in wildtype and connexin43 knockout mice. Dev Dyn 235(10):2786-2794. [PubMed: 16802337] [MGI Ref ID J:112454]
Ema M; Yokomizo T; Wakamatsu A; Terunuma T; Yamamoto M; Takahashi S. 2006. Primitive erythropoiesis from mesodermal precursors expressing VE-cadherin, PECAM-1, Tie2, endoglin, and CD34 in the mouse embryo. Blood 108(13):4018-24. [PubMed: 16926294] [MGI Ref ID J:140543]
Fathers KE; Stone CM; Minhas K; Marriott JJ; Greenwood JD; Dumont DJ; Coomber BL. 2005. Heterogeneity of Tie2 expression in tumor microcirculation: influence of cancer type, implantation site, and response to therapy. Am J Pathol 167(6):1753-62. [PubMed: 16314485] [MGI Ref ID J:107320]
Ferdous A; Caprioli A; Iacovino M; Martin CM; Morris J; Richardson JA; Latif S; Hammer RE; Harvey RP; Olson EN; Kyba M; Garry DJ. 2009. Nkx2-5 transactivates the Ets-related protein 71 gene and specifies an endothelial/endocardial fate in the developing embryo. Proc Natl Acad Sci U S A 106(3):814-9. [PubMed: 19129488] [MGI Ref ID J:143866]
Foteinos G; Hu Y; Xiao Q; Metzler B; Xu Q. 2008. Rapid endothelial turnover in atherosclerosis-prone areas coincides with stem cell repair in apolipoprotein E-deficient mice. Circulation 117(14):1856-63. [PubMed: 18378610] [MGI Ref ID J:153300]
Gontan C; de Munck A; Vermeij M; Grosveld F; Tibboel D; Rottier R. 2008. Sox2 is important for two crucial processes in lung development: branching morphogenesis and epithelial cell differentiation. Dev Biol 317(1):296-309. [PubMed: 18374910] [MGI Ref ID J:136180]
Griffin CT; Srinivasan Y; Zheng YW; Huang W; Coughlin SR. 2001. A role for thrombin receptor signaling in endothelial cells during embryonic development. Science 293(5535):1666-70. [PubMed: 11533492] [MGI Ref ID J:71309]
Ii M; Nishimura H; Iwakura A; Wecker A; Eaton E; Asahara T; Losordo DW. 2005. Endothelial progenitor cells are rapidly recruited to myocardium and mediate protective effect of ischemic preconditioning via 'imported' nitric oxide synthase activity. Circulation 111(9):1114-20. [PubMed: 15723985] [MGI Ref ID J:108997]
Kim YH; Hu H; Guevara-Gallardo S; Lam MT; Fong SY; Wang RA. 2008. Artery and vein size is balanced by Notch and ephrin B2/EphB4 during angiogenesis. Development 135(22):3755-64. [PubMed: 18952909] [MGI Ref ID J:144857]
Kopp HG; Avecilla ST; Hooper AT; Shmelkov SV; Ramos CA; Zhang F; Rafii S. 2005. Tie2 activation contributes to hemangiogenic regeneration after myelosuppression. Blood 106(2):505-13. [PubMed: 15817675] [MGI Ref ID J:107474]
Li Z; Chen MJ; Stacy T; Speck NA. 2006. Runx1 function in hematopoiesis is required in cells that express Tek. Blood 107(1):106-10. [PubMed: 16174759] [MGI Ref ID J:125796]
Licht AH; Pein OT; Florin L; Hartenstein B; Reuter H; Arnold B; Lichter P; Angel P; Schorpp-Kistner M. 2006. JunB is required for endothelial cell morphogenesis by regulating core-binding factor beta. J Cell Biol 175(6):981-91. [PubMed: 17158955] [MGI Ref ID J:119115]
Lindsay EA; Baldini A. 2001. Recovery from arterial growth delay reduces penetrance of cardiovascular defects in mice deleted for the DiGeorge syndrome region. Hum Mol Genet 10(9):997-1002. [PubMed: 11309372] [MGI Ref ID J:119454]
Makinen T; Adams RH; Bailey J; Lu Q; Ziemiecki A; Alitalo K; Klein R; Wilkinson GA. 2005. PDZ interaction site in ephrinB2 is required for the remodeling of lymphatic vasculature. Genes Dev 19(3):397-410. [PubMed: 15687262] [MGI Ref ID J:95885]
Mayr U; Zou Y; Zhang Z; Dietrich H; Hu Y; Xu Q. 2006. Accelerated arteriosclerosis of vein grafts in inducible NO synthase(-/-) mice is related to decreased endothelial progenitor cell repair. Circ Res 98(3):412-20. [PubMed: 16385078] [MGI Ref ID J:118879]
Morimoto H; Takahashi M; Izawa A; Ise H; Hongo M; Kolattukudy PE; Ikeda U. 2006. Cardiac overexpression of monocyte chemoattractant protein-1 in transgenic mice prevents cardiac dysfunction and remodeling after myocardial infarction. Circ Res 99(8):891-9. [PubMed: 16990567] [MGI Ref ID J:126503]
Parmar N; Berry LR; Post M; Chan AK. 2009. Effect of covalent antithrombin-heparin complex on developmental mechanisms in the lung. Am J Physiol Lung Cell Mol Physiol 296(3):L394-403. [PubMed: 19112103] [MGI Ref ID J:146359]
Qin G; Ii M; Silver M; Wecker A; Bord E; Ma H; Gavin M; Goukassian DA; Yoon YS; Papayannopoulou T; Asahara T; Kearney M; Thorne T; Curry C; Eaton L; Heyd L; Dinesh D; Kishore R; Zhu Y; Losordo DW. 2006. Functional disruption of alpha4 integrin mobilizes bone marrow-derived endothelial progenitors and augments ischemic neovascularization. J Exp Med 203(1):153-63. [PubMed: 16401693] [MGI Ref ID J:118824]
Ruppel KM; Willison D; Kataoka H; Wang A; Zheng YW; Cornelissen I; Yin L; Xu SM; Coughlin SR. 2005. Essential role for Galpha13 in endothelial cells during embryonic development. Proc Natl Acad Sci U S A 102(23):8281-6. [PubMed: 15919816] [MGI Ref ID J:99731]
Schmidt D; Textor B; Pein OT; Licht AH; Andrecht S; Sator-Schmitt M; Fusenig NE; Angel P; Schorpp-Kistner M. 2007. Critical role for NF-kappaB-induced JunB in VEGF regulation and tumor angiogenesis. EMBO J 26(3):710-9. [PubMed: 17255940] [MGI Ref ID J:119917]
Shaw AT; Meissner A; Dowdle JA; Crowley D; Magendantz M; Ouyang C; Parisi T; Rajagopal J; Blank LJ; Bronson RT; Stone JR; Tuveson DA; Jaenisch R; Jacks T. 2007. Sprouty-2 regulates oncogenic K-ras in lung development and tumorigenesis. Genes Dev 21(6):694-707. [PubMed: 17369402] [MGI Ref ID J:119477]
Xu Q; Zhang Z; Davison F; Hu Y. 2003. Circulating progenitor cells regenerate endothelium of vein graft atherosclerosis, which is diminished in ApoE-deficient mice. Circ Res 93(8):e76-86. [PubMed: 14512446] [MGI Ref ID J:115655]
Yamagishi H; Maeda J; Hu T; McAnally J; Conway SJ; Kume T; Meyers EN; Yamagishi C; Srivastava D. 2003. Tbx1 is regulated by tissue-specific forkhead proteins through a common Sonic hedgehog-responsive enhancer. Genes Dev 17(2):269-81. [PubMed: 12533514] [MGI Ref ID J:81296]
Zhou B; Ma Q; Kong SW; Hu Y; Campbell PH; McGowan FX; Ackerman KG; Wu B; Zhou B; Tevosian SG; Pu WT. 2009. Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart. J Clin Invest 119(6):1462-76. [PubMed: 19411759] [MGI Ref ID J:150452]
Zhu J; Motejlek K; Wang D; Zang K; Schmidt A; Reichardt LF. 2002. beta8 integrins are required for vascular morphogenesis in mouse embryos. Development 129(12):2891-903. [PubMed: 12050137] [MGI Ref ID J:77682]
van Tuyl M; Groenman F; Wang J; Kuliszewski M; Liu J; Tibboel D; Post M. 2007. Angiogenic factors stimulate tubular branching morphogenesis of sonic hedgehog-deficient lungs. Dev Biol 303(2):514-526. [PubMed: 17187775] [MGI Ref ID J:119174]
Health & husbandry
Health & Colony Maintenance Information
Animal Health Reports
Room Number FGB29
Colony Maintenance
Mating System Inbred x Hemizygote (Female x Male) 01-MAR-06 Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $239.00 Female or Male Hemizygous for Tg(TIE2-lacZ)182Sato
Pairs /Price (US dollars $) Pair Genotype $254.35 FVB/NJ (001800) x Hemizygous for Tg(TIE2-lacZ)182Sato $254.50 Hemizygous for Tg(TIE2-lacZ)182Sato x FVB/NJ (001800)
Additional Supply Details
| Pricing for International shipping destinations |
|
Weeks of Age Price (US dollars $) Gender Genotypes Provided Individual Mouse $310.70 Female or Male Hemizygous for Tg(TIE2-lacZ)182Sato
Pairs /Price (US dollars $) Pair Genotype $330.70 FVB/NJ (001800) x Hemizygous for Tg(TIE2-lacZ)182Sato $330.90 Hemizygous for Tg(TIE2-lacZ)182Sato x FVB/NJ (001800)
Additional Supply Details
|
|
|
Supply Details
| Standard Supply | Repository-Live. A collection of over 1000 strains maintained as live colonies. Individual colonies are sized to meet current customer demand. Delivery for orders of 10 mice or less ranges on average from one to eight weeks; mice are generally shipped between four to six weeks of age with a maximum shipping age of approximately nine weeks. Colony sizes do not generally support stringent age specifications for large volumes of mice; however custom orders and larger quantities of mice are easily arranged. Estimated ship dates for all orders provided within two business days following order placement. |
|---|---|
| Supply Notes |
|
| Important Note | |
| This strain is homozygous for the retinal degeneration allele Pde6brd1. | |
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| 001800 FVB/NJ | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
Payment Terms and Conditions
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
See Terms of Use tab for General Terms and Conditions
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
Purchasing Information
JAX® Mice Orders
Surgical Services
Contact Information
Orders & Technical Support
Tel: 1-800-422-6423 or 1-207-288-5845
Fax: 1-207-288-6150
Technical Support Email Form
Terms of Use
Terms of Use
General Terms and Conditions
Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice, Products & Services Conditions of Use
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.No Warranty
MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.
No Liability
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. In purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.