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- Description
- Phenotype
- Genes & alleles
- Genotyping
- Health & husbandry
- References
- Purchasing information
- Terms of Use
Description
The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.
Strain Information
Former Names STOCK Tg(balancer1)2Cgn/J (Changed: 06-MAR-06 ) Type Mutant Stock; Transgenic; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Species laboratory mouse Generation +N2 Donating Investigator Klaus Rajewsky, Harvard Medical School Description
These transgenic mice carry the Cre recombinase gene under the control of the rat nestin promoter. When crossed with a strain carrying a gene flanked by loxP sites, partial deletion of the loxP-flanked allele occurs before embryonic day 10.5 and is detectable in all adult organs examined including germ line cells. Because of the partial deletion, these balancer1 cre transgenic mice, as well as the balancer2 cre transgenic mice (Stock No. 002859), may be used to generate mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest. The proportion of cells that carry the mutant allele varies between tissues and between individual mice. In cases where deletion of a particular gene is lethal, the generation of mosaic mice using these strains can bypass lethality in some mosaic individuals. The balancer2 line induces more variable deletion of loxP-flanked genes than the balancer1 line.Development
The transgene contains the gene for Cre recombinase under the control of the rat nestin promoter and second intron enhancer. The "SLNE4c" construct was used. B6CBAF2 embryos were injected. This strain was submitted on a mixed C57BL/6, CBA, BALB/c, 129 background.
Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
Related Strains
Strains carrying other alleles of Nes
004127 FVB-Tg(Nes-rtTA)306Rvs/J 002859 STOCK Tg(Nes-cre)2Wme/J View Strains carrying other alleles of Nes (2 strains)
Additional Web Information
Cre-lox Systems
Phenotype
Phenotype Information
This mouse can be used to support research in many areas including:
cre relatedNeurobiology Research
Cre-lox System (Cre Recombinase expression in neural tissue)
Research Tools
Cre-lox System (Cre Recombinase Expression)
Developmental Biology Research (Cre-lox System)
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Reproductive Biology Research (Cre-lox System)
Research Tools
Cre-lox System
Genetics Research (Mutagenesis and Transgenesis: Cre-lox System)
Genes & Alleles
Gene & Allele Information
| Allele Symbol | Tg(Nes-cre)1Wme | ||
|---|---|---|---|
| Allele Name | transgene insertion 1, Werner Muller | ||
| Allele Type | Transgenic (Cre/Flp) | ||
| Common Name(s) | Tg(balancer1)2Cgn; TgN(balancer1)1Cgn; TgN(balancer1)2Cgn; bal1; balancer1; | ||
| Mutation Made By | Ulrich Betz, Business Group Pharma/Research | ||
| Strain of Origin | (C57BL/6 x CBA)F2 | ||
| Site of Expression | induces variable mosaicism/partial deletion of loxP-flanked alleles before embryonic day 10.5 and in all adult organs (including germline), producing mosaic mice consisting of cells that are either wildtype or mutant for the gene of interest | ||
| Expressed Gene | cre, cre recombinase, bacteriophage P1 | ||
| Cre recombinase is an enzyme derived from the bacteriophage P1 that specifically recognizes loxP sites. Cre has been shown to effectively mediate the excision of DNA located between loxP sites. After the excision event, the DNA ends recombine leaving a single loxP site in place of the intervening sequence. | |||
| Promoter | Nes, nestin, mouse, laboratory | ||
| General Note | Mice carrying this "balancer 1" transgene show less variability and instability in their generation of mosaics than do transgenic mice carrying the "balancer 2" transgene. | ||
| Molecular Note | The transgenic construct contained a nestin promoter followed by cre recombinase sequence adjoined to a nuclear localization signal. Intron 2 of the nestin gene was included upstream of a polyadenylation signal. While the endogenous nestin promoter is CNS-specific, Southern blot analysis showed the ubiquitous expression of cre in transgenic mice. Variable mosaicism/partial deletion of loxP-flanked alleles occurs before embryonic day 10.5, and it is detectable in all adult organs examined including germ line cells. [MGI Ref ID J:67926] | ||
| Gene Symbol and Name | Tg(Nes-cre)1Wme, transgene insertion 1, Werner Muller | ||
| Chromosome | UN | ||
| Gene Common Name(s) | Tg(balancer1)2Cgn; TgN(balancer1)1Cgn; TgN(balancer1)2Cgn; bal1; balancer1; | ||
Genotyping
Genotyping Information
Genotyping Protocols
Generic Cre, STD PCR, vers. 1
Helpful Links
Optimizing PCR Protocols
References
References
Selected Reference(s)
Betz UA; Vosshenrich CA; Rajewsky K; Muller W. 1996. Bypass of lethality with mosaic mice generated by Cre-loxP-mediated recombination. Curr Biol 6(10):1307-16. [PubMed: 8939573] [MGI Ref ID J:67926]
Additional References
Alonzi T; Middleton G; Wyatt S; Buchman V; Betz UA; Muller W; Musiani P; Poli V; Davies AM. 2001. Role of STAT3 and PI 3-kinase/Akt in mediating the survival actions of cytokines on sensory neurons. Mol Cell Neurosci 18(3):270-82. [PubMed: 11591128] [MGI Ref ID J:86898]
Hallonet M; Kaestner KH; Martin-Parras L; Sasaki H; Betz UA; Ang SL. 2002. Maintenance of the specification of the anterior definitive endoderm and forebrain depends on the axial mesendoderm: a study using HNF3beta/Foxa2 conditional mutants. Dev Biol 243(1):20-33. [PubMed: 11846474] [MGI Ref ID J:75055]
Tg(Nes-cre)1Wme relatedAlonzi T; Middleton G; Wyatt S; Buchman V; Betz UA; Muller W; Musiani P; Poli V; Davies AM. 2001. Role of STAT3 and PI 3-kinase/Akt in mediating the survival actions of cytokines on sensory neurons. Mol Cell Neurosci 18(3):270-82. [PubMed: 11591128] [MGI Ref ID J:86898]
Arenkiel BR; Klein ME; Davison IG; Katz LC; Ehlers MD. 2008. Genetic control of neuronal activity in mice conditionally expressing TRPV1. Nat Methods 5(4):299-302. [PubMed: 18327266] [MGI Ref ID J:136582]
Behrens A; Haigh J; Mechta-Grigoriou F; Nagy A; Yaniv M; Wagner EF. 2003. Impaired intervertebral disc formation in the absence of Jun. Development 130(1):103-9. [PubMed: 12441295] [MGI Ref ID J:80022]
Ben-Haim N; Lu C; Guzman-Ayala M; Pescatore L; Mesnard D; Bischofberger M; Naef F; Robertson EJ; Constam DB. 2006. The nodal precursor acting via activin receptors induces mesoderm by maintaining a source of its convertases and BMP4. Dev Cell 11(3):313-23. [PubMed: 16950123] [MGI Ref ID J:112799]
Collins BJ; Deak M; Murray-Tait V; Storey KG; Alessi DR. 2005. In vivo role of the phosphate groove of PDK1 defined by knockin mutation. J Cell Sci 118(Pt 21):5023-34. [PubMed: 16219676] [MGI Ref ID J:102388]
Forni PE; Scuoppo C; Imayoshi I; Taulli R; Dastru W; Sala V; Betz UA; Muzzi P; Martinuzzi D; Vercelli AE; Kageyama R; Ponzetto C. 2006. High levels of Cre expression in neuronal progenitors cause defects in brain development leading to microencephaly and hydrocephaly. J Neurosci 26(37):9593-602. [PubMed: 16971543] [MGI Ref ID J:112466]
Frappart PO; Tong WM; Demuth I; Radovanovic I; Herceg Z; Aguzzi A; Digweed M; Wang ZQ. 2005. An essential function for NBS1 in the prevention of ataxia and cerebellar defects. Nat Med 11(6):538-44. [PubMed: 15821748] [MGI Ref ID J:98316]
Hallonet M; Kaestner KH; Martin-Parras L; Sasaki H; Betz UA; Ang SL. 2002. Maintenance of the specification of the anterior definitive endoderm and forebrain depends on the axial mesendoderm: a study using HNF3beta/Foxa2 conditional mutants. Dev Biol 243(1):20-33. [PubMed: 11846474] [MGI Ref ID J:75055]
Koike M; Shibata M; Tadakoshi M; Gotoh K; Komatsu M; Waguri S; Kawahara N; Kuida K; Nagata S; Kominami E; Tanaka K; Uchiyama Y. 2008. Inhibition of autophagy prevents hippocampal pyramidal neuron death after hypoxic-ischemic injury. Am J Pathol 172(2):454-69. [PubMed: 18187572] [MGI Ref ID J:131320]
Komatsu M; Waguri S; Chiba T; Murata S; Iwata J; Tanida I; Ueno T; Koike M; Uchiyama Y; Kominami E; Tanaka K. 2006. Loss of autophagy in the central nervous system causes neurodegeneration in mice. Nature 441(7095):880-4. [PubMed: 16625205] [MGI Ref ID J:110051]
Komatsu M; Waguri S; Koike M; Sou YS; Ueno T; Hara T; Mizushima N; Iwata J; Ezaki J; Murata S; Hamazaki J; Nishito Y; Iemura S; Natsume T; Yanagawa T; Uwayama J; Warabi E; Yoshida H; Ishii T; Kobayashi A; Yamamoto M; Yue Z; Uchiyama Y; Kominami E; TanakaK. 2007. Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. Cell 131(6):1149-63. [PubMed: 18083104] [MGI Ref ID J:130839]
Milosevic J; Maisel M; Wegner F; Leuchtenberger J; Wenger RH; Gerlach M; Storch A; Schwarz J. 2007. Lack of hypoxia-inducible factor-1 alpha impairs midbrain neural precursor cells involving vascular endothelial growth factor signaling. J Neurosci 27(2):412-21. [PubMed: 17215402] [MGI Ref ID J:117302]
Okada S; Nakamura M; Katoh H; Miyao T; Shimazaki T; Ishii K; Yamane J; Yoshimura A; Iwamoto Y; Toyama Y; Okano H. 2006. Conditional ablation of Stat3 or Socs3 discloses a dual role for reactive astrocytes after spinal cord injury. Nat Med 12(7):829-34. [PubMed: 16783372] [MGI Ref ID J:111976]
Shillingford JM; Murcia NS; Larson CH; Low SH; Hedgepeth R; Brown N; Flask CA; Novick AC; Goldfarb DA; Kramer-Zucker A; Walz G; Piontek KB; Germino GG; Weimbs T. 2006. The mTOR pathway is regulated by polycystin-1, and its inhibition reverses renal cystogenesis in polycystic kidney disease. Proc Natl Acad Sci U S A 103(14):5466-71. [PubMed: 16567633] [MGI Ref ID J:108293]
Sklyarova T; Bonne S; D'Hooge P; Denecker G; Goossens S; De Rycke R; Borgonie G; Bosl M; van Roy F; van Hengel J. 2008. Plakophilin-3-deficient mice develop hair coat abnormalities and are prone to cutaneous inflammation. J Invest Dermatol 128(6):1375-85. [PubMed: 18079750] [MGI Ref ID J:136451]
Song MR; Shirasaki R; Cai CL; Ruiz EC; Evans SM; Lee SK; Pfaff SL. 2006. T-Box transcription factor Tbx20 regulates a genetic program for cranial motor neuron cell body migration. Development 133(24):4945-55. [PubMed: 17119020] [MGI Ref ID J:119655]
Souabni A; Jochum W; Busslinger M. 2007. Oncogenic role of Pax5 in the T-lymphoid lineage upon ectopic expression from the immunoglobulin heavy-chain locus. Blood 109(1):281-9. [PubMed: 16968900] [MGI Ref ID J:118111]
Yang YG; Frappart PO; Frappart L; Wang ZQ; Tong WM. 2006. A novel function of DNA repair molecule Nbs1 in terminal differentiation of the lens fibre cells and cataractogenesis. DNA Repair (Amst) 5(8):885-93. [PubMed: 16790366] [MGI Ref ID J:112192]
Health & husbandry
Health & Colony Maintenance Information
Colony Maintenance
Diet Information LabDiet® 5K52/5K67
Purchasing information
Pricing, Supply Level & Notes, Controls, General Terms & Conditions
Pricing
| Pricing for USA, Canada and Mexico shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $1900.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
| Pricing for International shipping destinations |
|
Animals Provided
Price (US dollars $) Cryorecovery Fee $2470.00 At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.
Additional Supply Details
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Supply Details
| Standard Supply | Cryopreserved. Ready for recovery. Please refer to pricing and supply notes for further information. |
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| Supply Notes |
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Control Information
| Control | ||
|---|---|---|
| Noncarrier | ||
| Considerations for Choosing Controls | ||
| USA, Canada and Mexico - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
| International - Control Pricing Information for Genetically Engineered Mutant Strains. | ||
General Terms and Conditions
See Terms of Use
The Jackson Laboratory's Genotype Promise
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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Terms of Use
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General Terms and Conditions
For Licensing and Use Restrictions view the link(s) below:
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Contact information
General inquiries
Contracts Administration
| phone: | 207-288-6470 |
| fax: | 207-288-6655 |
JAX® Mice & Services Conditions of Use
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