Strain Name:

129-Wnt4tm1Amc/J

Stock Number:

002866

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Strain; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
Visit our online Nomenclature tutorial.
Specieslaboratory mouse
 
Donating Investigator Andrew P McMahon,   University of Southern California

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ (approximate)
   000691 129X1/SvJ (approximate)
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Wnt4tm1Amc allele
006010   B6Ei.129-Wnt4tm1Amc/Ei
View Strains carrying   Wnt4tm1Amc     (1 strain)

Strains carrying other alleles of Wnt4
007032   B6;129S-Wnt4tm1.1Bhr/BhrEiJ
View Strains carrying other alleles of Wnt4     (1 strain)

Additional Web Information

New 129 Nomenclature Bulletin

Phenotype

Phenotype Information

View Related Disease (OMIM) Terms

Related Disease (OMIM) Terms provided by MGI
- Potential model based on gene homology relationships. Phenotypic similarity to the human disease has not been tested.
46,xx Sex Reversal with Dysgenesis of Kidneys, Adrenals, and Lungs;   (WNT4)
Mullerian Aplasia and Hyperandrogenism   (WNT4)
View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Wnt4tm1Amc/Wnt4tm1Amc

        involves: 129S1/Sv
  • mortality/aging
  • complete neonatal lethality
    • homozygotes die within 24 hrs of birth due to kidney failure   (MGI Ref ID J:21884)
    • however, homozygotes are recovered at the expected Mendelian frequency at late stages of gestation   (MGI Ref ID J:21884)
  • renal/urinary system phenotype
  • abnormal metanephric mesenchyme morphology
    • at E18.5, agenic kidneys consist of undifferentiated mesenchyme interspersed with branches of collecting duct epithelium   (MGI Ref ID J:21884)
    • at E15, 30% of kidneys show no histological signs of mesenchymal aggregation and lack pretubular aggregates or more developed tubules; the rest exhibit a few poorly developed pretubular aggregates   (MGI Ref ID J:21884)
  • abnormal nephrogenic mesenchyme morphogenesis
    • at E15, no comma-shaped or S-shaped bodies (stage II nephrons) are observed, unlike in wild-type embryos   (MGI Ref ID J:21884)
  • absent kidney
    • all E18.5 and newborn homozygotes display agenic kidneys   (MGI Ref ID J:21884)
  • delayed kidney development
    • at E15, kidneys are growth retarded and the mesenchyme persists in an undifferentiated state   (MGI Ref ID J:21884)
  • kidney failure   (MGI Ref ID J:21884)
  • reproductive system phenotype
  • abnormal female germ cell morphology   (MGI Ref ID J:52554)
    • decreased oocyte number
      • at birth, mutant ovaries exhibit oocyte depletion with less than 10% of the oocyte numbers scored in wild-type or heterozygous ovaries   (MGI Ref ID J:52554)
    • oocyte degeneration
      • the few oocytes detected at birth are in the process of degeneration   (MGI Ref ID J:52554)
  • abnormal oogenesis
    • female homozygotes exhibit reduced oocyte development   (MGI Ref ID J:52554)
    • decreased oocyte number
      • at birth, mutant ovaries exhibit oocyte depletion with less than 10% of the oocyte numbers scored in wild-type or heterozygous ovaries   (MGI Ref ID J:52554)
  • abnormal ovary morphology
    • at birth, the female gonad is rounded, lacks a capsule, and develops closely associated with a fat pad thus resembling the male gonad   (MGI Ref ID J:52554)
    • in contrast to the masculinized ovary and proximal sex ducts, female external genitalia develop normally   (MGI Ref ID J:52554)
  • abnormal secondary sex determination
    • Wolffian duct development and Mullerian duct regression are normal in mutant males but both also occur in mutant females   (MGI Ref ID J:52554)
    • secondary sex reversal
      • at E18.5, the gonad and proximal sex ducts of mutant females appear masculinized, Mullerian ducts are absent, and two Wolffian specific duct cell markers (Pax2 and Shh) identify a single ovary-associated duct with a highly convoluted proximal region resembling the epididymal region of the male Wolffian duct   (MGI Ref ID J:52554)
      • unlike the wild-type ovary, Sertoli cell markers (MIS and Dhh) are ectopically expressed in the sex cords of mutant females at birth, indicating a partial somatic sex reversal in the absence of oocytes   (MGI Ref ID J:52554)
      • in addition, patchy ectopic expression of Leydig cell markers (3Beta-HSD and 17alpha-hydroxylase) is noted in the ovary from E14.5 until birth   (MGI Ref ID J:52554)
  • hematopoietic system phenotype
  • decreased thymocyte number
    • at E15-E16, homozygotes show a 20-30% reduction in thymocyte number relative to wild-type embryos   (MGI Ref ID J:75999)
    • however, T cell maturation during fetal development appears unaffected   (MGI Ref ID J:75999)
  • immune system phenotype
  • decreased thymocyte number
    • at E15-E16, homozygotes show a 20-30% reduction in thymocyte number relative to wild-type embryos   (MGI Ref ID J:75999)
    • however, T cell maturation during fetal development appears unaffected   (MGI Ref ID J:75999)
  • embryogenesis phenotype
  • absent Mullerian ducts
    • at E14.5, Mullerian duct development is absent in females and specific Mullerian cell markers (Wnt7a and Pax8) are not detected   (MGI Ref ID J:52554)
  • endocrine/exocrine gland phenotype
  • abnormal ovary morphology
    • at birth, the female gonad is rounded, lacks a capsule, and develops closely associated with a fat pad thus resembling the male gonad   (MGI Ref ID J:52554)
    • in contrast to the masculinized ovary and proximal sex ducts, female external genitalia develop normally   (MGI Ref ID J:52554)
  • decreased thymocyte number
    • at E15-E16, homozygotes show a 20-30% reduction in thymocyte number relative to wild-type embryos   (MGI Ref ID J:75999)
    • however, T cell maturation during fetal development appears unaffected   (MGI Ref ID J:75999)
  • nervous system phenotype
  • *normal* nervous system phenotype
    • mice exhibit normal numbers of thoracic motor neurons and proportions of motor columnar subtypes   (MGI Ref ID J:155074)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Wnt4tm1Amc/Wnt4tm1Amc

        involves: 129/Sv * CD-1
  • endocrine/exocrine gland phenotype
  • abnormal adenohypophysis morphology
    • the anterior pituitary is nearly devoid of all cell types except ACTH-expressing corticotropes   (MGI Ref ID J:48144)
    • decreased gonadotroph cell number   (MGI Ref ID J:48144)
    • decreased somatotroph cell number   (MGI Ref ID J:48144)
    • decreased thyrotroph cell number   (MGI Ref ID J:48144)
  • abnormal adrenal gland physiology
    • adrenal glands appear morphologically normal but there is reduced aldosterone production in adrenal cortex   (MGI Ref ID J:80420)
    • ectopic expression of adrenal gland- pecific Cyp21 in gonads suggests abnormal migration of adrenal cells in early development   (MGI Ref ID J:80420)
  • nervous system phenotype
  • abnormal adenohypophysis morphology
    • the anterior pituitary is nearly devoid of all cell types except ACTH-expressing corticotropes   (MGI Ref ID J:48144)
    • decreased gonadotroph cell number   (MGI Ref ID J:48144)
    • decreased somatotroph cell number   (MGI Ref ID J:48144)
    • decreased thyrotroph cell number   (MGI Ref ID J:48144)

Wnt4tm1Amc/Wnt4tm1Amc

        involves: 129/Sv * C57BL/6
  • renal/urinary system phenotype
  • decreased renal glomerulus number   (MGI Ref ID J:98831)
  • small metanephros
    • severely hypoplastic metanephroi, that can form a few glomeruli, nephron tubules and medulla-like structures   (MGI Ref ID J:98831)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Wnt4tm1Amc related

Developmental Biology Research
Internal/Organ Defects
      kidney

Internal/Organ Research
Kidney Defects

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Wnt4tm1Amc
Allele Name targeted mutation 1, Andrew P McMahon
Allele Type Targeted (Null/Knockout)
Common Name(s) Wnt-4-; Wnt4n;
Mutation Made By Andrew McMahon,   University of Southern California
Strain of Origin129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
ES Cell Line NameCJ7
ES Cell Line Strain129S1/Sv-Oca2<+> Tyr<+> Kitl<+>
Gene Symbol and Name Wnt4, wingless-type MMTV integration site family, member 4
Chromosome 4
Gene Common Name(s) SERKAL; WNT-4; Wnt-4;
Molecular Note Exon 3, encoding amino acids 106 through 196, was replaced by a PGK-neo cassette. The occasional expression of the targeted locus was observed in poorly developed aggregates in the kidneys of homozygous mutant mice via in situ hybridization. Expression was unaltered in the central nervous system and mesonephric mesenchyme. [MGI Ref ID J:21884]

Genotyping

Genotyping Information

Genotyping Protocols

Wnt4tm1Amc, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Additional References

Wnt4tm1Amc related

Agalliu D; Takada S; Agalliu I; McMahon AP; Jessell TM. 2009. Motor neurons with axial muscle projections specified by Wnt4/5 signaling. Neuron 61(5):708-20. [PubMed: 19285468]  [MGI Ref ID J:155074]

Boyle SC; Kim M; Valerius MT; McMahon AP; Kopan R. 2011. Notch pathway activation can replace the requirement for Wnt4 and Wnt9b in mesenchymal-to-epithelial transition of nephron stem cells. Development 138(19):4245-54. [PubMed: 21852398]  [MGI Ref ID J:176046]

Brisken C; Heineman A; Chavarria T; Elenbaas B; Tan J; Dey SK; McMahon JA; McMahon AP; Weinberg RA. 2000. Essential function of Wnt-4 in mammary gland development downstream of progesterone signaling. Genes Dev 14(6):650-4. [PubMed: 10733525]  [MGI Ref ID J:61384]

Burn SF; Webb A; Berry RL; Davies JA; Ferrer-Vaquer A; Hadjantonakis AK; Hastie ND; Hohenstein P. 2011. Calcium/NFAT signalling promotes early nephrogenesis. Dev Biol 352(2):288-98. [PubMed: 21295565]  [MGI Ref ID J:171469]

Chassot AA; Bradford ST; Auguste A; Gregoire EP; Pailhoux E; de Rooij DG; Schedl A; Chaboissier MC. 2012. WNT4 and RSPO1 together are required for cell proliferation in the early mouse gonad. Development 139(23):4461-72. [PubMed: 23095882]  [MGI Ref ID J:189064]

Chassot AA; Ranc F; Gregoire EP; Roepers-Gajadien HL; Taketo MM; Camerino G; de Rooij DG; Schedl A; Chaboissier MC. 2008. Activation of beta-catenin signaling by Rspo1 controls differentiation of the mammalian ovary. Hum Mol Genet 17(9):1264-77. [PubMed: 18250098]  [MGI Ref ID J:133933]

Correa SM; Washburn LL; Kahlon RS; Musson MC; Bouma GJ; Eicher EM; Albrecht KH. 2012. Sex reversal in C57BL/6J XY mice caused by increased expression of ovarian genes and insufficient activation of the testis determining pathway. PLoS Genet 8(4):e1002569. [PubMed: 22496664]  [MGI Ref ID J:183829]

Coveney D; Ross AJ; Slone JD; Capel B. 2007. A microarray analysis of the XX Wnt4 mutant gonad targeted at the identification of genes involved in testis vascular differentiation.(Correction: 2008; 8:529-537) Gene Expr Patterns 7(1-2):82-92. [PubMed: 16844427]  [MGI Ref ID J:116022]

DeFalco T; Takahashi S; Capel B. 2011. Two distinct origins for Leydig cell progenitors in the fetal testis. Dev Biol 352(1):14-26. [PubMed: 21255566]  [MGI Ref ID J:171480]

Fleming A; Ghahramani N; Zhu MX; Delot EC; Vilain E. 2012. Membrane beta-catenin and adherens junctions in early gonadal patterning. Dev Dyn 241(11):1782-98. [PubMed: 22972715]  [MGI Ref ID J:187965]

Garcia-Ortiz JE; Pelosi E; Omari S; Nedorezov T; Piao Y; Karmazin J; Uda M; Cao A; Cole SW; Forabosco A; Schlessinger D; Ottolenghi C. 2009. Foxl2 functions in sex determination and histogenesis throughout mouse ovary development. BMC Dev Biol 9:36. [PubMed: 19538736]  [MGI Ref ID J:152858]

Heikkila M; Peltoketo H; Leppaluoto J; Ilves M; Vuolteenaho O; Vainio S. 2002. Wnt-4 deficiency alters mouse adrenal cortex function, reducing aldosterone production. Endocrinology 143(11):4358-65. [PubMed: 12399432]  [MGI Ref ID J:80420]

Heikkila M; Prunskaite R; Naillat F; Itaranta P; Vuoristo J; Leppaluoto J; Peltoketo H; Vainio S. 2005. The partial female to male sex reversal in Wnt-4-deficient females involves induced expression of testosterone biosynthetic genes and testosterone production, and depends on androgen action. Endocrinology 146(9):4016-23. [PubMed: 15932923]  [MGI Ref ID J:129451]

Heinonen KM; Vanegas JR; Brochu S; Shan J; Vainio SJ; Perreault C. 2011. Wnt4 regulates thymic cellularity through the expansion of thymic epithelial cells and early thymic progenitors. Blood 118(19):5163-73. [PubMed: 21937690]  [MGI Ref ID J:178890]

Itaranta P; Chi L; Seppanen T; Niku M; Tuukkanen J; Peltoketo H; Vainio S. 2006. Wnt-4 signaling is involved in the control of smooth muscle cell fate via Bmp-4 in the medullary stroma of the developing kidney. Dev Biol 293(2):473-83. [PubMed: 16546160]  [MGI Ref ID J:108713]

Jameson SA; Lin YT; Capel B. 2012. Testis development requires the repression of Wnt4 by Fgf signaling. Dev Biol 370(1):24-32. [PubMed: 22705479]  [MGI Ref ID J:188056]

Jeays-Ward K; Dandonneau M; Swain A. 2004. Wnt4 is required for proper male as well as female sexual development. Dev Biol 276(2):431-40. [PubMed: 15581876]  [MGI Ref ID J:106304]

Jeays-Ward K; Hoyle C; Brennan J; Dandonneau M; Alldus G; Capel B; Swain A. 2003. Endothelial and steroidogenic cell migration are regulated by WNT4 in the developing mammalian gonad. Development 130(16):3663-70. [PubMed: 12835383]  [MGI Ref ID J:84602]

Karner CM; Das A; Ma Z; Self M; Chen C; Lum L; Oliver G; Carroll TJ. 2011. Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development. Development 138(7):1247-57. [PubMed: 21350016]  [MGI Ref ID J:171512]

Kashimada K; Pelosi E; Chen H; Schlessinger D; Wilhelm D; Koopman P. 2011. FOXL2 and BMP2 act cooperatively to regulate follistatin gene expression during ovarian development. Endocrinology 152(1):272-80. [PubMed: 21084449]  [MGI Ref ID J:168597]

Kim Y; Kobayashi A; Sekido R; DiNapoli L; Brennan J; Chaboissier MC; Poulat F; Behringer RR; Lovell-Badge R; Capel B. 2006. Fgf9 and Wnt4 act as antagonistic signals to regulate mammalian sex determination. PLoS Biol 4(6):e187. [PubMed: 16700629]  [MGI Ref ID J:110252]

Kispert A; Vainio S; Shen L; Rowitch DH; McMahon AP. 1996. Proteoglycans are required for maintenance of Wnt-11 expression in the ureter tips. Development 122(11):3627-37. [PubMed: 8951078]  [MGI Ref ID J:36828]

Kobayashi A; Kwan KM; Carroll TJ; McMahon AP; Mendelsohn CL; Behringer RR. 2005. Distinct and sequential tissue-specific activities of the LIM-class homeobox gene Lim1 for tubular morphogenesis during kidney development. Development 132(12):2809-23. [PubMed: 15930111]  [MGI Ref ID J:98831]

Kobayashi A; Shawlot W; Kania A; Behringer RR. 2004. Requirement of Lim1 for female reproductive tract development. Development 131(3):539-49. [PubMed: 14695376]  [MGI Ref ID J:133086]

Kobayashi A; Stewart CA; Wang Y; Fujioka K; Thomas NC; Jamin SP; Behringer RR. 2011. {beta}-Catenin is essential for Mullerian duct regression during male sexual differentiation. Development 138(10):1967-75. [PubMed: 21490063]  [MGI Ref ID J:171430]

Liu CF; Parker K; Yao HH. 2010. WNT4/beta-catenin pathway maintains female germ cell survival by inhibiting activin betaB in the mouse fetal ovary. PLoS One 5(4):e10382. [PubMed: 20454446]  [MGI Ref ID J:160549]

Louis I; Heinonen KM; Chagraoui J; Vainio S; Sauvageau G; Perreault C. 2008. The signaling protein Wnt4 enhances thymopoiesis and expands multipotent hematopoietic progenitors through beta-catenin-independent signaling. Immunity 29(1):57-67. [PubMed: 18617424]  [MGI Ref ID J:137842]

Maatouk DM; DiNapoli L; Alvers A; Parker KL; Taketo MM; Capel B. 2008. Stabilization of beta-catenin in XY gonads causes male-to-female sex-reversal. Hum Mol Genet 17(19):2949-55. [PubMed: 18617533]  [MGI Ref ID J:138861]

Maatouk DM; Mork L; Chassot AA; Chaboissier MC; Capel B. 2013. Disruption of mitotic arrest precedes precocious differentiation and transdifferentiation of pregranulosa cells in the perinatal Wnt4 mutant ovary. Dev Biol 383(2):295-306. [PubMed: 24036309]  [MGI Ref ID J:203797]

Manuylov NL; Smagulova FO; Leach L; Tevosian SG. 2008. Ovarian development in mice requires the GATA4-FOG2 transcription complex. Development 135(22):3731-43. [PubMed: 18927154]  [MGI Ref ID J:143586]

Mulroy T; McMahon JA; Burakoff SJ; McMahon AP; Sen J. 2002. Wnt-1 and Wnt-4 regulate thymic cellularity. Eur J Immunol 32(4):967-71. [PubMed: 11920562]  [MGI Ref ID J:75999]

Naillat F; Prunskaite-Hyyrylainen R; Pietila I; Sormunen R; Jokela T; Shan J; Vainio SJ. 2010. Wnt4/5a signalling coordinates cell adhesion and entry into meiosis during presumptive ovarian follicle development. Hum Mol Genet 19(8):1539-50. [PubMed: 20106871]  [MGI Ref ID J:158526]

Ottolenghi C; Pelosi E; Tran J; Colombino M; Douglass E; Nedorezov T; Cao A; Forabosco A; Schlessinger D. 2007. Loss of Wnt4 and Foxl2 leads to female-to-male sex reversal extending to germ cells. Hum Mol Genet 16(23):2795-804. [PubMed: 17728319]  [MGI Ref ID J:129972]

Park JS; Valerius MT; McMahon AP. 2007. Wnt/{beta}-catenin signaling regulates nephron induction during mouse kidney development. Development 134(13):2533-9. [PubMed: 17537789]  [MGI Ref ID J:122521]

Pazin DE; Albrecht KH. 2009. Developmental expression of Smoc1 and Smoc2 suggests potential roles in fetal gonad and reproductive tract differentiation. Dev Dyn 238(11):2877-90. [PubMed: 19842175]  [MGI Ref ID J:153731]

Perantoni AO; Timofeeva O; Naillat F; Richman C; Pajni-Underwood S; Wilson C; Vainio S; Dove LF; Lewandoski M. 2005. Inactivation of FGF8 in early mesoderm reveals an essential role in kidney development. Development 132(17):3859-71. [PubMed: 16049111]  [MGI Ref ID J:101175]

Potok MA; Cha KB; Hunt A; Brinkmeier ML; Leitges M; Kispert A; Camper SA. 2008. WNT signaling affects gene expression in the ventral diencephalon and pituitary gland growth. Dev Dyn 237(4):1006-20. [PubMed: 18351662]  [MGI Ref ID J:132979]

Saitoh A; Hansen LA; Vogel JC; Udey MC. 1998. Characterization of Wnt gene expression in murine skin: possible involvement of epidermis-derived Wnt-4 in cutaneous epithelial-mesenchymal interactions. Exp Cell Res 243(1):150-60. [PubMed: 9716459]  [MGI Ref ID J:49458]

Spater D; Hill TP; O'sullivan RJ; Gruber M; Conner DA; Hartmann C. 2006. Wnt9a signaling is required for joint integrity and regulation of Ihh during chondrogenesis. Development 133(15):3039-49. [PubMed: 16818445]  [MGI Ref ID J:119030]

Stark K; Vainio S; Vassileva G; McMahon AP. 1994. Epithelial transformation of metanephric mesenchyme in the developing kidney regulated by Wnt-4. Nature 372(6507):679-83. [PubMed: 7990960]  [MGI Ref ID J:21884]

Treier M; Gleiberman AS; O'Connell SM; Szeto DP; McMahon JA; McMahon AP; Rosenfeld MG. 1998. Multistep signaling requirements for pituitary organogenesis in vivo. Genes Dev 12(11):1691-704. [PubMed: 9620855]  [MGI Ref ID J:48144]

Tsaousi A; Williams H; Lyon CA; Taylor V; Swain A; Johnson JL; George SJ. 2011. Wnt4/beta-catenin signaling induces VSMC proliferation and is associated with intimal thickening. Circ Res 108(4):427-36. [PubMed: 21193738]  [MGI Ref ID J:183499]

Uhlenhaut NH; Jakob S; Anlag K; Eisenberger T; Sekido R; Kress J; Treier AC; Klugmann C; Klasen C; Holter NI; Riethmacher D; Schutz G; Cooney AJ; Lovell-Badge R; Treier M. 2009. Somatic sex reprogramming of adult ovaries to testes by FOXL2 ablation. Cell 139(6):1130-42. [PubMed: 20005806]  [MGI Ref ID J:157008]

Vainio S; Heikkila M; Kispert A; Chin N; McMahon AP. 1999. Female development in mammals is regulated by Wnt-4 signalling. Nature 397(6718):405-9. [PubMed: 9989404]  [MGI Ref ID J:52554]

Valerius MT; McMahon AP. 2008. Transcriptional profiling of Wnt4 mutant mouse kidneys identifies genes expressed during nephron formation. Gene Expr Patterns 8(5):297-306. [PubMed: 18346943]  [MGI Ref ID J:133949]

Yao HH; Aardema J; Holthusen K. 2006. Sexually dimorphic regulation of inhibin Beta B in establishing gonadal vasculature in mice. Biol Reprod 74(5):978-83. [PubMed: 16452457]  [MGI Ref ID J:107812]

Yao HH; Matzuk MM; Jorgez CJ; Menke DB; Page DC; Swain A; Capel B. 2004. Follistatin operates downstream of Wnt4 in mammalian ovary organogenesis. Dev Dyn 230(2):210-5. [PubMed: 15162500]  [MGI Ref ID J:90277]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2140.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $2782.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.

    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
   002448 129S1/SvImJ (approximate)
   000691 129X1/SvJ (approximate)
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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Terms of Use

Terms of Use


General Terms and Conditions


For Licensing and Use Restrictions view the link(s) below:
- Use of MICE by companies or for-profit entities requires a license prior to shipping.

Contact information

General inquiries regarding Terms of Use

Contracts Administration

phone:207-288-6470

JAX® Mice, Products & Services Conditions of Use

"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCTS" means biological materials supplied by JACKSON, and their derivatives. "RECIPIENT" means each recipient of MICE, PRODUCTS, or services provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than the internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE or PRODUCTS from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.

No Warranty

MICE, PRODUCTS AND SERVICES ARE PROVIDED “AS IS”. JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.

In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of mice, products or services, JACKSON will, at its option, provide credit or replacement for the mice or product received or the services provided.

No Liability

In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS or services, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS or services from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.

MICE and PRODUCTS are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.

The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or services. In addition, special terms and conditions of sale of certain MICE, PRODUCTS or services may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and services by JACKSON, and by its licensees and distributors.

Acceptance of delivery of MICE, PRODUCTS or services shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or services by JACKSON.


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