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Strain Name:

B6SJL-Tg(Wnt1)1Hev/J

Stock Number:

002870

Availability:

Repository-Cryopreserved

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Genes & Alleles   Wnt1;   MMTV;   Tg(Wnt1)1Hev;

Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Strain
Additional information on JAX® GEMM® Strains.
Type JAX® GEMM® Strain - Transgenic
Specieslaboratory mouse
Donating Investigator IMR Colony,   The Jackson Laboratory

Strain Description
Mammary glands from virgin hemizygous females carrying the (Wnt1)1Hev transgene resemble hormonally-stimulated glands from pregnant mice. There was a marked increase in numbers of terminal branches and alveoli, producing a diffuse lobular hyperplasia. Parous transgenic females were unable to lactate. Hemizygous transgenic male glands, while less developed, also were hyperplastic. Adenocarcinomas developed between 3 and 7 months in females and more rarely in males. Occasional metastatic lesions were observed in females. Salivary adenocarcinomas were also occasionally observed in both males and females. Tumors arose stochastically, indicating additional events are required for neoplasia. Subsequent work with Wnt1 transgenic mice includes infection with MMTV to identify contributing oncogenes and mating to transgenic mice bearing other oncogenes.

Strain Development
The construct contained an MMTV LTR about 1 kb upstream from a Wnt1 genomic clone, with SV40 splice and polyadenylation sites added at the 3' end. The construct was microinjected into (C57BL/6 X SJL)F1 fertilized eggs.

Mammalian Phenotype Terms assigned by genotype

Tg(Wnt1)1Hev/0

        either: FVB/N or (involves: C57BL/6 * SJL)
  • endocrine/exocrine gland phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:28473)
    • mammary glands in virgin females resemble hormonally-stimulated glands from pregnant mice
    • abnormal branching of the mammary ductal tree (MGI Ref ID J:28473)
      • hemizygous transgenic females show a marked increase in the number of terminal branches
    • mammary alveolar hyperplasia (MGI Ref ID J:28473)
    • mammary gland hyperplasia (MGI Ref ID J:28473)
      • hemizygous transgenic females display diffuse lobular hyperplasia
  • reproductive system phenotype
  • abnormal mammary gland morphology (MGI Ref ID J:28473)
    • mammary glands in virgin females resemble hormonally-stimulated glands from pregnant mice
    • abnormal branching of the mammary ductal tree (MGI Ref ID J:28473)
      • hemizygous transgenic females show a marked increase in the number of terminal branches
    • mammary alveolar hyperplasia (MGI Ref ID J:28473)
    • mammary gland hyperplasia (MGI Ref ID J:28473)
      • hemizygous transgenic females display diffuse lobular hyperplasia

The following phenotype information may relate to a genetic background differing from this JAX® Mice strain.

Tg(Wnt1)1Hev/0

        involves: C57BL/6 * FVB * SJL
  • endocrine/exocrine gland phenotype
  • abnormal branching of the mammary ductal tree (MGI Ref ID J:96305)
    • mammary glands of virgin females have excessive ductal side branching resembling that of pregnant wild type mice
  • mammary gland hyperplasia (MGI Ref ID J:96305)
    • mammary glands of virgin females have extensive, dense hyperplasia
  • reproductive system phenotype
  • abnormal branching of the mammary ductal tree (MGI Ref ID J:96305)
    • mammary glands of virgin females have excessive ductal side branching resembling that of pregnant wild type mice
  • mammary gland hyperplasia (MGI Ref ID J:96305)
    • mammary glands of virgin females have extensive, dense hyperplasia
  • tumorigenesis
  • mammary gland tumor (MGI Ref ID J:96305)
    • develop mammary tumors

Gene & Allele Details

Allele Symbol Tg(Wnt1)1Hev
Allele Name transgene insertion 1, Harold E Varmus
Common Name(s) MMTV-Wnt-1; Wnt-1; line 303;
Mutation Made By Harold Varmus,   Memorial Sloan-Kettering Cancer Center
Strain of OriginC57BL/6 x SJL
Expressed Gene Wnt1, wingless-related MMTV integration site 1, mouse, laboratory
Promoter MMTV, Mouse Mammary Tumor Virus, MMTV
Molecular Note Wnt1 is driven by the mouse mammary tumor virus (MMTV). The construct resembles alleles found in virus-induced tumors. [MGI Ref ID J:28473]

Control Information

  Control
   100012 B6SJLF1/J
 
  Considerations for Choosing Controls

Genotyping Protocols

Tg(Wnt1)1Hev

Colony Maintenance

Breeding & HusbandryHemizygotes are fertile, but hemizygous females do not lactate. When maintaining a live colony, transgenic males can be mated to B6SJLF1/J (JR#100012) females. Expected coat color from breeding:Black, White Bellied Agouti, Light Gray and Tan w/ pink eyes.
Diet Information LabDiet® 5K52/5K67

Related Strains

Strains carrying   Tg(Wnt1)1Hev allele
002934   FVB.Cg-Tg(Wnt1)1Hev/J
View Strains carrying   Tg(Wnt1)1Hev     (1 strain)

Strains carrying other alleles of Wnt1
000243   B6C3Fe a/a-Wnt1sw/J
002865   B6CBA-Tg(Wnt1-lacZ)206Amc/J
003829   STOCK Tg(Wnt1-cre)11Rth Tg(Wnt1-GAL4)11Rth/J
View Strains carrying other alleles of Wnt1     (3 strains)

View Strains carrying other alleles of MMTV     (16 strains)

Research Applications

This mouse can be used to support research in many areas including:

Cancer Research
Increased Tumor Incidence (Mammary Gland Tumors)

References

Selected Reference(s)

Tsukamoto AS; Grosschedl R; Guzman RC; Parslow T; Varmus HE. 1988. Expression of the int-1 gene in transgenic mice is associated with mammary gland hyperplasia and adenocarcinomas in male and female mice. Cell 55(4):619-25. [PubMed: 3180222]  [MGI Ref ID J:28473]

Additional References

Price and Supply Information

Strain Name: B6SJL-Tg(Wnt1)1Hev/J
Stock Number: 002870

Price Details

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Supply Details

Standard SupplyRepository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes Cryorecovery - Standard.
The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice.
One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org.
This strain is included in the Induced Mutant Resource Colony collection.
Genomic DNA is available for this strain from the Mouse DNA Resource.

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Control InformationView Control Information in Strain Details.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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