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Genes & Alleles

Apob;   Apobtm1Sgy;

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Product Information

Strain Details

Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Stephen Young,   Gladstone Institutes UCSF Generation F?+5p (09-NOV-05)

Appearance
white-bellied agouti
Related Genotype: A^w/A^w

Strain Description
Mice homozygous for the Apob ^tm1Sgy targeted mutation are viable and fertile. They show normal expression of APOB48 in the gut but have no expression of APOB100. Homozygous mice show no developmental defects indicating that APOB100 synthesis in the yolk sac is not necessary for normal development. Homozygotes have lower serum LDL cholesterol levels compared to wildtype and APOB100 expressing mice (B6,129-Apob ^tm2Sgy, Stock No. 002877).

Related Disease (OMIM) Terms Apolipoprotein B; APOB

Mammalian Phenotype Terms assigned by genotype Apobtm1Sgy/Apobtm1Sgy         involves: 129/Sv * C57BL/6 homeostasis/metabolism phenotype decreased circulating LDL cholesterol level (MGI Ref ID J:33830) decreased circulating triglyceride level (MGI Ref ID J:33830)

Gene & Allele Details

Allele Symbol		Apobtm1Sgy
Allele Name		targeted mutation 1, Stephen G Young
Common Name(s)		ApoB-48-; ApoB48; apo-B48;
Mutation Made By		Stephen Young,   Gladstone Institutes UCSF
Strain of Origin		129S7/SvEvBrd-Hprt1<+>
ES Cell Line Name		AB1
ES Cell Line Strain		129S7/SvEvBrd-Hprt1<+>
Gene Symbol and Name		Apob, apolipoprotein B
Chromosome		12
Gene Common Name(s)		AI315052; Aa1064; Ac1-060; ApoB-100; ApoB-48; FLDB; expressed sequence AI315052;
General Note		By targeted mutagenesis of the editing codon, mice expressing only APOB48 or only APOB100 were obtained. Both types of mice develop normally and are healthy and fertile. Levels of LDL-cholesterol, VLDL, and triglyceride LDL are lower relative to wild type mice in APOB48 only mice and higher in APOB100 only mice in the presence of wild type APOE production. In the presence of genetic APOE deficiency, APOB100 only mice have lowered cholesterol levels (J:33830). APOB48 only mice have higher levels of cholesterol and more atherosclerotic lesions than APOB100 only mice in the absence of APOE (J:41510).
Molecular Note		A "hit and run"-type vector was used to create a stop signal in codon 2153 in sequences corresponding to the apo-B48 editing codon. Western blot analysis on plasma derived from heterozygous and homozygous mice demostrated that the expression of the ApoB48 isoform is unaffected by this mutation, while no ApoB100 isoform is produced from this allele. [MGI Ref ID J:33830]

Control Information

Allele	Control
Apobtm1Sgy	101045 B6129SF2/J	(approximate)
Considerations for Choosing Controls

Genotyping Protocols

Apob^tm1Sgy

Colony Maintenance

Breeding & Husbandry	This strain is maintained by homozygous sibling matings. Expected coat color from breeding:Black, White Bellied Agouti
Diet Information	LabDiet® 5K52/5K67

Related Strains

Strains carrying   Apob^tm1Sgy allele

002878

B6;129-Apobtm1Sgy Apoetm1Unc/J

View Strains carrying   Apob^tm1Sgy     (1 strain)

Strains carrying other alleles of Apob

002053	B6.129P2-Apobtm1Unc/J
007682	B6.129X1-Apobtm1.1Zc/J
002879	B6;129-Apobtm2Sgy Apoetm1Unc/J
003000	B6;129S-Apobtm2Sgy Ldlrtm1Her/J
002877	B6;129S7-Apobtm2Sgy/J
007683	CByJ.129X1(Cg)-Apobtm1.1Zc/J
004192	STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J
007679	SWR.129X1(B6)-Apobtm1.1Zc/J

View Strains carrying other alleles of Apob     (8 strains)

Research Applications

This mouse can be used to support research in many areas including:

Apob^tm1Sgy related

Cardiovascular Research
Atherosclerosis
Hypocholesterolemia

Mouse/Human Gene Homologs
hypobetalipoproteinemia, familial

References

Selected Reference(s)

Farese RVJr; Veniant MM; Cham CM; Flynn LM; Pierotti V; Loring JF; Traber M; Ruland S; Stokowski RS; Huszar D; Young SG. 1996. Phenotypic analysis of mice expressing exclusively apolipoprotein B48 or apolipoprotein B100. Proc Natl Acad Sci U S A 93(13):6393-8. [PubMed: 8692825]  [MGI Ref ID J:33830]

Additional References

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Price and Supply Information

Strain Name:	B6;129S-Apobtm1Sgy/J
Stock Number:	002876

Price Details

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Supply Details

Standard Supply	Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information.
Supply Notes	Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. One to two pairs will be recovered to establish a Dedicated Supply of mice. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services: Tel: 1-800-422-6423 or 1-207-288-5845; Email: jaxservices@jax.org. This strain is included in the Induced Mutant Resource Colony collection. Genomic DNA is available for this strain from the Mouse DNA Resource.
Licensing	See General Terms and Conditions below for Licensing and Use Restrictions
Control Information	View Control Information in Strain Details.

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- Use of MICE by companies or for-profit entities requires a license prior to shipping.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX^® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX^® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX^® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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