Strain Name: |
B6;129-Apobtm1Sgy Apoetm1Unc/J |
|---|---|
Stock Number: |
002878 |
Availability: | Repository-Cryopreserved |
General Terms and Conditions |
| Genes & Alleles | Apob; Apobtm1Sgy; Apoe; Apoetm1Unc; |
Product Information
Strain Details
Type JAX® GEMM® Strain - Mutant Stock Additional information on JAX® GEMM® Strains. Type JAX® GEMM® Strain - Targeted Mutation Species laboratory mouse Donating Investigator Stephen Young, Gladstone Institutes UCSF Generation F?+12 Appearance
pink-eyed grey
Related Genotype: a/a Oca2p/Oca2pStrain Description
Mice homozygous for the Apobtm1Sgy and Apoetm1Unc targeted mutations do not express the apolipoproteins B100 or E. They do retain the ability to express apolipoprotein B48. They have the highest total cholesterol (392 mg/dl vs 341 for Apobtm1Unc/Apoetm1Unc); highest LDL cholesterol (APOB48 is not a ligand for LDLR), and atherosclerotic lesions more extensive than Apoetm1Unc/Apoetm1Unc homozygotes. The extent of atherosclerosis correlated significantly with plasma cholesterol levels.Strain Development
The "apo-B48-only" mice were generated using site-directed mutagenesis to introduce an oligonucleotide containing a stop codon into codon 2153 of the Apob gene. The neo resistance gene was also inserted to be used as a selectable marker. These mice were subsequently crossed to Apoetm1Unc mice to generate doubly knocked out mice. The Apobtm1Sgy targeted mutation was generated in a 129 steel derived ES cell line while the Apoetm1Unc mutation was generated in 129P2/OlaHsd-derived E14TG2a ES cells.
Gene & Allele Details
| Allele Symbol | Apobtm1Sgy | ||
|---|---|---|---|
| Allele Name | targeted mutation 1, Stephen G Young | ||
| Common Name(s) | ApoB-48-; ApoB48; apo-B48; | ||
| Mutation Made By | Stephen Young, Gladstone Institutes UCSF | ||
| Strain of Origin | 129S7/SvEvBrd-Hprt1<+> | ||
| ES Cell Line Name | AB1 | ||
| ES Cell Line Strain | 129S7/SvEvBrd-Hprt1<+> | ||
| Gene Symbol and Name | Apob, apolipoprotein B | ||
| Chromosome | 12 | ||
| Gene Common Name(s) | AI315052; Aa1064; Ac1-060; ApoB-100; ApoB-48; FLDB; expressed sequence AI315052; | ||
| General Note | By targeted mutagenesis of the editing codon, mice expressing only APOB48 or only APOB100 were obtained. Both types of mice develop normally and are healthy and fertile. Levels of LDL-cholesterol, VLDL, and triglyceride LDL are lower relative to wild type mice in APOB48 only mice and higher in APOB100 only mice in the presence of wild type APOE production. In the presence of genetic APOE deficiency, APOB100 only mice have lowered cholesterol levels (J:33830). APOB48 only mice have higher levels of cholesterol and more atherosclerotic lesions than APOB100 only mice in the absence of APOE (J:41510). | ||
| Molecular Note | A "hit and run"-type vector was used to create a stop signal in codon 2153 in sequences corresponding to the apo-B48 editing codon. Western blot analysis on plasma derived from heterozygous and homozygous mice demostrated that the expression of the ApoB48 isoform is unaffected by this mutation, while no ApoB100 isoform is produced from this allele. [MGI Ref ID J:33830] | ||
| Allele Symbol | Apoetm1Unc | ||
| Allele Name | targeted mutation 1, University of North Carolina | ||
| Common Name(s) | APOE KO; AopE(-); ApoE-KO; Apoetm1Un; apoE-; apoE0; epsilon-; | ||
| Mutation Made By | Nobuyo Maeda, Univ of North Carolina at Chapel Hill | ||
| Strain of Origin | 129P2/OlaHsd | ||
| ES Cell Line Name | E14TG2a | ||
| ES Cell Line Strain | 129P2/OlaHsd | ||
| Gene Symbol and Name | Apoe, apolipoprotein E | ||
| Chromosome | 7 | ||
| Gene Common Name(s) | AD2; AI255918; APOEA; LPG; MGC1571; apoprotein; expressed sequence AI255918; | ||
| General Note |
In Apoetm1Unc mutant mice also carrying Csf1op, atherotic lesions of the proximal aorta were abolished or significantly reduced in size relative to Apoe mutants homozygous for the normal allele at Csf1 (J:40136). The results may have been due to reduced macrophages or M-CSF, but were not due to reductions in circulating lipoprotein (J:40136). | ||
| Molecular Note | Insertion of a neomycin resistance cassette deleted part of exon 3 and part of intron 3 of the Apoe gene. Plasma from homozygous mutant mice gave no detectable immunoprecipitate by the Ouchterlony double immunodiffusion test using a rabbit antibody to rat APOE. [MGI Ref ID J:1050] | ||
Control Information
| Control | ||
|---|---|---|
| 100903 B6129PF2/J | (approximate) | |
| 101045 B6129SF2/J | (approximate) | |
| Considerations for Choosing Controls | ||
Genotyping Protocols
Apobtm1Sgy
Apoetm1Unc
Colony Maintenance
| Breeding & Husbandry | When maintaining a live colony, this strain is maintained by mating homozygous siblings. Expected coat color from breeding:Tan w/pink eyes |
|---|---|
| Diet Information | LabDiet® 5K52/5K67 |
Related Strains
Strains carrying Apobtm1Sgy allele
002876 B6;129S-Apobtm1Sgy/J View Strains carrying Apobtm1Sgy (1 strain)
002053 B6.129P2-Apobtm1Unc/J 007682 B6.129X1-Apobtm1.1Zc/J 002879 B6;129-Apobtm2Sgy Apoetm1Unc/J 003000 B6;129S-Apobtm2Sgy Ldlrtm1Her/J 002877 B6;129S7-Apobtm2Sgy/J 007683 CByJ.129X1(Cg)-Apobtm1.1Zc/J 004192 STOCK Mttptm2Sgy Ldlrtm1Her Apobtm2Sgy Tg(Mx1-cre)1Cgn/J 007679 SWR.129X1(B6)-Apobtm1.1Zc/J
Research Applications
This mouse can be used to support research in many areas including:
Apobtm1Sgy relatedNeurobiology Research
Behavioral and Learning Defects
Neurodegeneration
Apoetm1Unc relatedCardiovascular Research
Atherosclerosis
Hypocholesterolemia
Mouse/Human Gene Homologs
hypobetalipoproteinemia, familial
Cardiovascular Research
Atherosclerosis
Hypercholesterolemia
Mouse/Human Gene Homologs
Alzheimer's
hyperlipoproteinemia, type III
References
Selected Reference(s)
Additional ReferencesFarese RVJr; Veniant MM; Cham CM; Flynn LM; Pierotti V; Loring JF; Traber M; Ruland S; Stokowski RS; Huszar D; Young SG. 1996. Phenotypic analysis of mice expressing exclusively apolipoprotein B48 or apolipoprotein B100. Proc Natl Acad Sci U S A 93(13):6393-8. [PubMed: 8692825] [MGI Ref ID J:33830]
Price and Supply Information
| Strain Name: | B6;129-Apobtm1Sgy Apoetm1Unc/J |
| Stock Number: | 002878 |
Price Details
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Supply Details
| Standard Supply | Repository-Cryopreserved. Must Be Recovered. Please refer to pricing and supply notes for further information. |
|---|---|
| Supply Notes |
Cryorecovery - Standard. The recovery process begins when a signed agreement form is returned to the Customer Service Department after order placement. Although results vary by strain, at least two males and two females (two pairs) will be provided, typically within 15 weeks of our receipt of the signed agreement form. If the first recovery attempt is unsuccessful or only one pair is recovered, a second recovery will be done, extending the delivery time to approximately 25 weeks. At least one member of each pair will be of known genotype and will carry the mutation if it is a mutant strain. Please note that pairs may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation of the strain. Mating schemes are sometimes modified for successful cryopreservation. Price represents a repository maintenance fee, which includes the cost of recovery of the strain from the cryopreservation resource and the periodic replacement of the frozen embryos used for recovery. Cryorecovery to establish a Dedicated Supply for greater quantities of mice. |
| Licensing | See General Terms and Conditions below for Licensing and Use Restrictions |
| Control Information | View Control Information in Strain Details. |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.Ordering and Purchasing Information
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