Strain Name:

STOCK Gria2tm1Rod/J

Stock Number:

002913

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Description

The genotypes of the animals provided may not reflect those discussed in the strain description or the mating scheme utilized by The Jackson Laboratory prior to cryopreservation. Please inquire for possible genotypes for this specific strain.

Strain Information

Type Mutant Stock; Targeted Mutation;
Additional information on Genetically Engineered and Mutant Mice.
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Specieslaboratory mouse
 
Donating InvestigatorDr. John Roder,   University of Toronto

Description
Mice homozygous for the Gria2tm1Rod targeted mutation (GluR2) were originally reported to be about one half the size of their normal littermates by two to three weeks leading to some preweaning mortality. Growth retardation and mortality can be diminished by reducing litter size and eliminating some competition for the mother's milk. Homozygotes do appear similar in size and weight by eight weeks of age. Neurons lacking Gria2 exhibit a 9-fold increase in Ca2+ permeability and enhanced long-term potentiation (LTP). Antagonists of AMPA and NMDA receptors block all the enhanced LTP seen in homozygous null mice. Unpublished data show changes in pain thresholds, sensitivity to anaesthetics, focal cerebral ischemia, apoptosis of neuronal subpopulations and learning impairments.

It has been the experience of The Jackson Laboratory that no homozygous animals can be recovered from heterozygous sibling matings.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls

Related Strains

Strains carrying   Gria2tm1Rod allele
003143   B6.129-Gria2tm1Rod/J
View Strains carrying   Gria2tm1Rod     (1 strain)

Strains carrying other alleles of Gria2
012615   B6.129-Gria2tm1Rlh/J
View Strains carrying other alleles of Gria2     (1 strain)

Phenotype

Phenotype Information

View Mammalian Phenotype Terms

Mammalian Phenotype Terms provided by MGI
      assigned by genotype

Gria2tm1Rod/Gria2tm1Rod

        involves: 129S1/Sv * 129X1/SvJ * CD-1
  • mortality/aging
  • partial postnatal lethality
    • by 2 - 3 weeks of age about 20% of homozygotes die   (MGI Ref ID J:64275)
  • behavior/neurological phenotype
  • abnormal reflex
    • impaired eye closure reflex to approaching objects   (MGI Ref ID J:64275)
  • abnormal response to novel object
    • object exploration is reduced   (MGI Ref ID J:64275)
  • decreased exploration in new environment   (MGI Ref ID J:64275)
  • decreased grooming behavior   (MGI Ref ID J:64275)
  • impaired coordination
    • impaired coordination on rotorod test; however no signs of seizure activity were detected by observation, EEG, or post-mortem pyramidal cell dropout in the hippocampus   (MGI Ref ID J:64275)
  • nervous system phenotype
  • enhanced long term potentiation
    • LTP in CA1 region of the hippocampus is enhanced 2-fold and non-saturating   (MGI Ref ID J:64275)
  • growth/size/body phenotype
  • decreased body size
    • body size is reduced at 2 - 3 weeks of age; however by 6 - 7 weeks, surviving homozygotes were the same size as littermates   (MGI Ref ID J:64275)
    • decreased body weight
      • body weight is reduced at 2 - 3 weeks of age; however by 6 - 7 weeks, surviving homozygotes were the same weight as littermates   (MGI Ref ID J:64275)

The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.

Gria2tm1Rod/Gria2+

        B6.129-Gria2tm1Rod/J
  • nervous system phenotype
  • abnormal AMPA-mediated synaptic currents
    • high degree of inhibition by the external polyamine 1-naphtyl acetyl spermine (NAS), a strong inward rectification and a high relative Ca2+ permeability   (MGI Ref ID J:104951)
    • higher elevations of the intracellular Ca2+ concentration upon AMPA receptor stimulation   (MGI Ref ID J:104951)
    • normal number of AMPA receptors in the cell membrane   (MGI Ref ID J:104951)
  • increased susceptibility to neuronal excitotoxicity
    • increased vulnerability to AMPA receptor-mediated excitotoxicity   (MGI Ref ID J:104951)
  • homeostasis/metabolism phenotype
  • increased susceptibility to neuronal excitotoxicity
    • increased vulnerability to AMPA receptor-mediated excitotoxicity   (MGI Ref ID J:104951)
  • cellular phenotype
  • increased susceptibility to neuronal excitotoxicity
    • increased vulnerability to AMPA receptor-mediated excitotoxicity   (MGI Ref ID J:104951)

Gria2tm1Rod/Gria2tm1Rod

        B6.129-Gria2tm1Rod/J
  • nervous system phenotype
  • abnormal AMPA-mediated synaptic currents
    • high degree of inhibition by the external NAS, a strong inward rectification and a high relative Ca2+ permeability   (MGI Ref ID J:104951)
    • higher elevations of the intracellular Ca2+ concentration upon AMPA receptor stimulation   (MGI Ref ID J:104951)
    • normal number of AMPA receptors in the cell membrane   (MGI Ref ID J:104951)
  • increased susceptibility to neuronal excitotoxicity
    • increased vulnerability to AMPA receptor-mediated excitotoxicity   (MGI Ref ID J:104951)
    • abolished AMPA receptor-mediated cell death in cultured motor neurons by the external polyamine 1-naphtyl acetyl spermine (NAS)   (MGI Ref ID J:104951)
  • behavior/neurological phenotype
  • abnormal limb posture
    • abnormal posturing of both forelimbs and hind limbs during manipulations   (MGI Ref ID J:104951)
  • abnormal response to novel object
    • reduced object exploration   (MGI Ref ID J:104951)
  • decreased grip strength
    • slightly lower grip strength in fore limbs   (MGI Ref ID J:104951)
  • hypoactivity
    • reduced spontaneous activity during the night   (MGI Ref ID J:104951)
  • impaired coordination
    • unable to walk on a rotarod   (MGI Ref ID J:104951)
  • homeostasis/metabolism phenotype
  • increased susceptibility to neuronal excitotoxicity
    • increased vulnerability to AMPA receptor-mediated excitotoxicity   (MGI Ref ID J:104951)
    • abolished AMPA receptor-mediated cell death in cultured motor neurons by the external polyamine 1-naphtyl acetyl spermine (NAS)   (MGI Ref ID J:104951)
  • cellular phenotype
  • increased susceptibility to neuronal excitotoxicity
    • increased vulnerability to AMPA receptor-mediated excitotoxicity   (MGI Ref ID J:104951)
    • abolished AMPA receptor-mediated cell death in cultured motor neurons by the external polyamine 1-naphtyl acetyl spermine (NAS)   (MGI Ref ID J:104951)
View Research Applications

Research Applications
This mouse can be used to support research in many areas including:

Gria2tm1Rod related

Cell Biology Research
Channel and Transporter Defects

Neurobiology Research
Channel and Transporter Defects
      sodium/potassium
Epilepsy
Receptor Defects
      glutamate receptor: ionotropic

Genes & Alleles

Gene & Allele Information provided by MGI

 
Allele Symbol Gria2tm1Rod
Allele Name targeted mutation 1, John Roder
Allele Type Targeted (knock-out)
Common Name(s) GluA2; GluR2-;
Mutation Made ByDr. John Roder,   University of Toronto
Strain of Origin(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
ES Cell Line NameR1
ES Cell Line Strain(129X1/SvJ x 129S1/Sv)F1-Kitl<+>
Gene Symbol and Name Gria2, glutamate receptor, ionotropic, AMPA2 (alpha 2)
Chromosome 3
Gene Common Name(s) GLUR2; GLURB; GluA2; GluR-B; GluR-K2; Glur-2; Glur2; HBGR2; glutamate receptor 2 (alpha 2);
Molecular Note A neomycin resistance cassette replaced exon 11 of the gene, which encodes the pore loop and transmembrane region, TM1. Western blots of brain plasma membranes from homozygous mutant mice showed no detectable protein for the targeted gene. [MGI Ref ID J:64275]

Genotyping

Genotyping Information

Genotyping Protocols

Gria2tm1Rod, Standard PCR


Helpful Links

Genotyping resources and troubleshooting

References

References provided by MGI

Selected Reference(s)

Jia Z; Agopyan N; Miu P; Xiong Z; Henderson J; Gerlai R; Taverna FA; Velumian A; MacDonald J; Carlen P; Abramow-Newerly W; Roder J. 1996. Enhanced LTP in mice deficient in the AMPA receptor GluR2. Neuron 17(5):945-56. [PubMed: 8938126]  [MGI Ref ID J:64275]

Additional References

Hollmann M; Heinemann S. 1994. Cloned glutamate receptors. Annu Rev Neurosci 17:31-108. [PubMed: 8210177]  [MGI Ref ID J:17259]

Mead AN; Stephens DN. 2003. Involvement of AMPA receptor GluR2 subunits in stimulus-reward learning: evidence from glutamate receptor gria2 knock-out mice. J Neurosci 23(29):9500-7. [PubMed: 14573529]  [MGI Ref ID J:86306]

Gria2tm1Rod related

Adesnik H; Nicoll RA. 2007. Conservation of glutamate receptor 2-containing AMPA receptors during long-term potentiation. J Neurosci 27(17):4598-602. [PubMed: 17460072]  [MGI Ref ID J:121085]

Biou V; Bhattacharyya S; Malenka RC. 2008. Endocytosis and recycling of AMPA receptors lacking GluR2/3. Proc Natl Acad Sci U S A 105(3):1038-43. [PubMed: 18195348]  [MGI Ref ID J:131171]

Gerlai R; Henderson JT; Roder JC; Jia Z. 1998. Multiple behavioral anomalies in GluR2 mutant mice exhibiting enhanced LTP. Behav Brain Res 95(1):37-45. [PubMed: 9754875]  [MGI Ref ID J:78386]

Gladding CM; Collett VJ; Jia Z; Bashir ZI; Collingridge GL; Molnar E. 2009. Tyrosine dephosphorylation regulates AMPAR internalisation in mGluR-LTD. Mol Cell Neurosci 40(2):267-79. [PubMed: 19063969]  [MGI Ref ID J:146934]

Herring BE; Shi Y; Suh YH; Zheng CY; Blankenship SM; Roche KW; Nicoll RA. 2013. Cornichon proteins determine the subunit composition of synaptic AMPA receptors. Neuron 77(6):1083-96. [PubMed: 23522044]  [MGI Ref ID J:197912]

Hu RQ; Cortez MA; Man HY; Roder J; Jia Z; Wang YT; Snead OC 3rd. 2001. Gamma-hydroxybutyric acid-induced absence seizures in GluR2 null mutant mice. Brain Res 897(1-2):27-35. [PubMed: 11282355]  [MGI Ref ID J:68428]

Iihara K; Joo DT; Henderson J; Sattler R; Taverna FA; Lourensen S; Orser BA; Roder JC; Tymianski M. 2001. The influence of glutamate receptor 2 expression on excitotoxicity in Glur2 null mutant mice. J Neurosci 21(7):2224-39. [PubMed: 11264298]  [MGI Ref ID J:68447]

Jackson AC; Nicoll RA. 2011. Stargazin (TARP {gamma}-2) Is Required for Compartment-Specific AMPA Receptor Trafficking and Synaptic Plasticity in Cerebellar Stellate Cells. J Neurosci 31(11):3939-52. [PubMed: 21411637]  [MGI Ref ID J:170457]

Joo DT; Gong D; Sonner JM; Jia Z; MacDonald JF; Eger EI 2nd; Orser BA. 2001. Blockade of AMPA receptors and volatile anesthetics: reduced anesthetic requirements in GluR2 null mutant mice for loss of the righting reflex and antinociception but not minimum alveolar concentration. Anesthesiology 94(3):478-88. [PubMed: 11374610]  [MGI Ref ID J:106294]

Joo DT; Xiong Z; MacDonald JF; Jia Z; Roder J; Sonner J; Orser BA. 1999. Blockade of glutamate receptors and barbiturate anesthesia: increased sensitivity to pentobarbital-induced anesthesia despite reduced inhibition of AMPA receptors in GluR2 null mutant mice [see comments] Anesthesiology 91(5):1329-41. [PubMed: 10551584]  [MGI Ref ID J:59684]

Lu W; Shi Y; Jackson AC; Bjorgan K; During MJ; Sprengel R; Seeburg PH; Nicoll RA. 2009. Subunit composition of synaptic AMPA receptors revealed by a single-cell genetic approach. Neuron 62(2):254-68. [PubMed: 19409270]  [MGI Ref ID J:155070]

Mainen ZF; Jia Z; Roder J; Malinow R. 1998. Use-dependent AMPA receptor block in mice lacking GluR2 suggests postsynaptic site for LTP expression. Nat Neurosci 1(7):579-86. [PubMed: 10196565]  [MGI Ref ID J:50786]

Mead AN; Brown G; Le Merrer J; Stephens DN. 2005. Effects of deletion of gria1 or gria2 genes encoding glutamatergic AMPA-receptor subunits on place preference conditioning in mice. Psychopharmacology (Berl) 179(1):164-71. [PubMed: 15619119]  [MGI Ref ID J:110154]

Mead AN; Stephens DN. 2003. Involvement of AMPA receptor GluR2 subunits in stimulus-reward learning: evidence from glutamate receptor gria2 knock-out mice. J Neurosci 23(29):9500-7. [PubMed: 14573529]  [MGI Ref ID J:86306]

Medvedev NI; Rodriguez-Arellano JJ; Popov VI; Davies HA; Tigaret CM; Schoepfer R; Stewart MG. 2008. The glutamate receptor 2 subunit controls post-synaptic density complexity and spine shape in the dentate gyrus. Eur J Neurosci 27(2):315-25. [PubMed: 18215230]  [MGI Ref ID J:132175]

Meng Y; Zhang Y; Jia Z. 2003. Synaptic transmission and plasticity in the absence of AMPA glutamate receptor GluR2 and GluR3. Neuron 39(1):163-76. [PubMed: 12848940]  [MGI Ref ID J:84336]

Panicker S; Brown K; Nicoll RA. 2008. Synaptic AMPA receptor subunit trafficking is independent of the C terminus in the GluR2-lacking mouse. Proc Natl Acad Sci U S A 105(3):1032-7. [PubMed: 18195349]  [MGI Ref ID J:131170]

Taylor EW; Wang K; Nelson AR; Bredemann TM; Fraser KB; Clinton SM; Puckett R; Marchase RB; Chatham JC; McMahon LL. 2014. O-GlcNAcylation of AMPA receptor GluA2 is associated with a novel form of long-term depression at hippocampal synapses. J Neurosci 34(1):10-21. [PubMed: 24381264]  [MGI Ref ID J:205592]

Van Damme P; Braeken D; Callewaert G; Robberecht W; Van Den Bosch L. 2005. GluR2 deficiency accelerates motor neuron degeneration in a mouse model of amyotrophic lateral sclerosis. J Neuropathol Exp Neurol 64(7):605-12. [PubMed: 16042312]  [MGI Ref ID J:104951]

Wang S; Jia Z; Roder J; Murphy TH. 2002. AMPA receptor-mediated miniature synaptic calcium transients in GluR2 null mice. J Neurophysiol 88(1):29-40. [PubMed: 12091530]  [MGI Ref ID J:103180]

Yan J; Zhang Y; Jia Z; Taverna FA; McDonald RJ; Muller RU; Roder JC. 2002. Place-cell impairment in glutamate receptor 2 mutant mice. J Neurosci 22(3):RC204. [PubMed: 11826150]  [MGI Ref ID J:75591]

Zhou Z; Hu J; Passafaro M; Xie W; Jia Z. 2011. GluA2 (GluR2) regulates metabotropic glutamate receptor-dependent long-term depression through N-cadherin-dependent and cofilin-mediated actin reorganization. J Neurosci 31(3):819-33. [PubMed: 21248105]  [MGI Ref ID J:168565]

Health & husbandry

Health & Colony Maintenance Information

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200.

Pricing and Purchasing

Pricing, Supply Level & Notes, Controls


Pricing for USA, Canada and Mexico shipping destinations View International Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $3175.00
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

Pricing for International shipping destinations View USA Canada and Mexico Pricing

Cryopreserved

Cryopreserved Mice - Ready for Recovery

Price (US dollars $)
Cryorecovery* $4127.50
Animals Provided

At least two mice that carry the mutation (if it is a mutant strain) will be provided. Their genotypes may not reflect those discussed in the strain description. Please inquire for possible genotypes and see additional details below.

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Supply Notes

  • Cryorecovery - Standard.
    Progeny testing is not required.
    The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided, their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders are needed. Animals typically ship between 11 and 14 weeks from the date of your order. If a second cryorecovery is needed in order to provide the minimum number of animals, animals will ship within 25 weeks. IMPORTANT NOTE: The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

    Cryorecovery to establish a Dedicated Supply for greater quantities of mice
    Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation. For more information on Dedicated Supply, please contact JAX® Services, Tel: 1-800-422-6423 (from U.S.A., Canada or Puerto Rico only) or 1-207-288-5845 (from any location).

View USA Canada and Mexico Pricing View International Pricing

Standard Supply

Cryopreserved. Ready for recovery. Please refer to pricing and supply notes on the strain data sheet for further information.

Control Information

  Control
   Wild-type from the colony
 
  Considerations for Choosing Controls
  Control Pricing Information for Genetically Engineered Mutant Strains.
 

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
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